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Статті в журналах з теми "Immunological strip"

Автор: Grafiati

Опубліковано: 3 червня 2025

Оновлено: 31 липня 2025

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1

Trojan, J., N. Povse, O. Schröder, and J. Stein. "A New Immunological Test Strip Device for the Rapid, Qualitative Detection of Faecal Occult Blood." Zeitschrift für Gastroenterologie 40, no. 11 (2002): 921–24. http://dx.doi.org/10.1055/s-2002-35415.

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2

Zhou, Xinghua, Shuoning Guan, Na Li, Jiayu Zhou, Wenwen Pan, and Yun Wang. "Development of Indirect Competitive ELISA and Colloidal Gold Immunochromatographic Strip for Endosulfan Detection Based on a Monoclonal Antibody." Foods 12, no. 4 (2023): 736. http://dx.doi.org/10.3390/foods12040736.

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Endosulfan, as an effective broad-spectrum insecticide, has been banned in agricultural areas because of the potential harmful effects on human health. This study aimed to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold immunochromatographic (ICA) strip based on a prepared monoclonal antibody (mAb) for quantitative and qualitative detection of endosulfan. A new mAb with high sensitivity and affinity was designed and screened. The ic-ELISA showed a 50% inhibition concentration (IC50) value of 5.16 ng/mL for endosulfan. Under optimum conditions, th

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3

MULDOON, MARK T., GEORGE TEANEY, JINGKUN LI, DALE V. ONISK, and JAMES W. STAVE. "Bacteriophage-Based Enrichment Coupled to Immunochromatographic Strip–Based Detection for the Determination of Salmonella in Meat and Poultry." Journal of Food Protection 70, no. 10 (2007): 2235–42. http://dx.doi.org/10.4315/0362-028x-70.10.2235.

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Immunochemical-based methods for the detection of Salmonella in food can be complicated by the presence of closely related, immunocrossreactive non-Salmonella species in the sample that may cause false-positive results. To circumvent this problem, specific bacteriophages against immunocrossreactive, non-Salmonella bacteria were used in the sample enrichment step to suppress their growth and improve the performance of an immunochromatographic strip–based detection method for Salmonella. Cross-reactive bacteria were isolated from various food sources and were characterized with a panel of Salmon

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4

HAMMAD, SAMAR M., IHAB A. NASER, MAHMMOUD H. TALEB, and AYMAN S. ABUTAIR. "Dietary intake and Biochemical Indicators and their association with Wound Healing Process among Adult Burned Patients in the Gaza Strip." Current Research in Nutrition and Food Science Journal 7, no. 1 (2019): 169–81. http://dx.doi.org/10.12944/crnfsj.7.1.17.

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Burn is a traumatic injury that causes immunological, endocrine, inflammatory, many metabolic responses and emotional stress which can affect dietary, micronutrients and antioxidants intake, which in turn have effects on recovery outcomes. To investigate the role of the nutrition and dietary intake on the progression of the different stages of the healing process among burned patients in Gaza strip. One hundred burned adult patients (36males and 64 females) were enrolled in this cross-sectional clinic-based study at Médecins Sans Frontières/ France clinics in Gaza Strip. Pretested interview qu

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5

Ren, Rui, Tao Wang, Le Gao, et al. "Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of Soybean mosaic virus." International Journal of Molecular Sciences 23, no. 16 (2022): 9457. http://dx.doi.org/10.3390/ijms23169457.

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Soybean is an important grain and oil crop worldwide; however, the yield and seed quality of which are seriously affected by Soybean mosaic virus (SMV). As efficient detection technology is crucial for the field management of SMV, novel immunological detection methods were developed in the present study. According to the phylogenetic analysis, the CP coding sequence of SMV-SC7 was selected for the prokaryotic expression of the recombinant SMV-CP. Purified SMV-CP was used for the development of polyclonal antibodies (PAb) against the SMV-CP (PAb-SMV-CP) and monoclonal antibodies (MAb) against S

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6

Lila, V. A. "Clinical and laboratory relationships in patients with different variants of the course of systemic lupus erythematosus." Modern Rheumatology Journal 14, no. 1 (2020): 26–31. http://dx.doi.org/10.14412/1996-7012-2020-1-26-31.

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Systemic lupus erythematosus (SLE) is characterized by a variability in clinical manifestations and laboratory abnormalities, which creates objective diagnostic difficulties.Objective: to investigate clinical and laboratory parameters in patients with definite and probable SLE.Patients and methods. The investigation enrolled 94 patients, 38 of whom were diagnosed with probable SLE. All the patients underwent immunological examination and unaffected skin biopsies, followed by an immunohistochemical study, the so-called lupus strip test (LST).Results and discussion. The group of patients with de

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7

Tan, You-wen, Jian-cheng Wu, Yun Ye, Li Chen, and Peng-li Pai. "Effects of Interleukin-18 and Anti-interleukin- 18-mAb on Experimental Immunological Liver Fibrosis Induced by Repeatedly Administered Concanavalin A and Its Mechanism." Infection International 3, no. 4 (2014): 161–72. http://dx.doi.org/10.1515/ii-2017-0092.

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Abstract Objective To explore the prevention of IL-18 or anti-IL-18-mAb to the immune liver fibrosis model induced by repeated injection of concanavalin A in BALB/c mice and its mechanism. Methods Total of 120 BALB/c mice were divided into four groups, control group mice (Ga) were injected weekly with normal saline, concanavalin A group was divided into Gb, Gc, Gd. All mice were injected with concanavalin A (15 mg/kg) once a week. Moreover, Gc, Gd mice were injected weekly with IL-18 (7.5 mg/kg) and anti-IL-18-mAb (10 mg/kg) 2 hours before treatment with concanavalin A, respectively. Twenty-fo

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8

Ezea Moses Chukwuemeka, Chukwuma Okechukwu George, Okere Philip, Onah Christian Ejike, Adonu Cyril, and Esimone Charles Okechukwu. "Impact of immunological, hematological and biochemical markers on discordant partners of Hepatitis B infection in Enugu State, Eastern Nigeria." World Journal of Biological and Pharmaceutical Research 1, no. 2 (2021): 029–39. http://dx.doi.org/10.53346/wjbpr.2021.1.2.0131.

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Globally, hepatitis B infection is a serious health problem as its morbities are difficult to handle with the commonly used antivirals. This study therefore aims to evaluate the serological, biochemical and hematological markers of Hepatitis B virus infection in a discordant partner in an attempt to aid proper diagnosis, management and control of the infection. This is a cross-sectional study consisting of 150 subjects recruited using a simple random sampling technique. HBsAg was screened using a rapid ELISA diagnostic strip and rescreened later for confirmation using Ichroma Fluorescence Immu

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9

Richter, Anne, Michaela Niemöller, Inken Verwohl, et al. "Rapid and Semi-Automated Screening of Multiple Samples for Antigen-Specific T Lymphocytes." Blood 120, no. 21 (2012): 4839. http://dx.doi.org/10.1182/blood.v120.21.4839.4839.

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Abstract Abstract 4839 Functional characterizations of T lymphocytes are performed to gain understanding on their contribution in certain immunological situations, to monitor the course of diseases, and to track therapeutic interventions. Meanwhile the flow cytometric analysis of antigen-specific T cells examined for intracellular cytokine production and expression of activation markers after a short-term in vitro antigenic challenge is a well-established method for research applications. Despite the advantages of this approach to qualify samples on a single cell level and by multiple paramete

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10

El Sheikha, Aly. "DNAFoil, a novel technology for the rapid detection of food pathogens: Preliminary validation on Salmonella and Listeria monocytogenes." Italian Journal of Food Science 33, SP1 (2021): 43–54. http://dx.doi.org/10.15586/ijfs.v33isp1.1995.

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Over the past decades, several tools have been developed for food pathogen detection, including proteomics, metabolomics, immunological, biosensor, and nucleic acid-based approaches. Although these techniques are reliable and precise, they are time-consuming, technically challenging, and costly. Hence, it is necessary to develop rapid techniques for food pathogen detection, which can be performed at the household level. DNAFoil mechanism is a portable, completely self-administered, on-site DNA test that does not need expensive instru-ments or settings to confirm food pathogen detection in as l

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11

Mezencev, S., and N. Mezenceva. "Diagnosis of heartworm in dogs." Bulletin of Science and Practice, no. 11 (November 13, 2017): 57–64. https://doi.org/10.5281/zenodo.1048297.

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Dirofilariasis refers to a group of diseases characterized by transmissible transmission, slow development, prolonged course and the formation of synanthropic and natural foci. The source of infection of dogs in synanthropic foci are mosquitoes. The generally accepted method of research on dirofilariasis is microscopy of blood with different reagents and dyes. This method is effective only in the circulation step microfilaria in venous blood of animals. For accurate and differential diagnosis of dirofilariasis, immunochromatographic test systems are used for rapid analysis of infestation. The

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12

Li, Yunxin, Ranran Huang, Aijie Wang, Guowei Zhang, and Yongbin Su. "Is Magnetic Resonance Imaging Able to Provide Specific Diagnoses? A Case Report of Clear Cell Sarcoma of Soft Tissue in the First Metacarpal of the Hand." Case Reports in Oncology 17, no. 1 (2024): 995–1000. http://dx.doi.org/10.1159/000540745.

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Introduction: Clear cell sarcoma (CCS) of the soft tissue is a type of tumor that primarily affects the deep soft tissues of the extremities and trunk. We report a case of CCS of soft tissue arising in the first metacarpal of the hand, focusing on the imaging features of CCS combined with the clinicopathological and immunological results. Case Presentation: In this case, computed tomography images showed a soft tissue mass at the first metacarpal, with heterogeneous density, unclear boundaries, and bone destruction. On magnetic resonance imaging (MRI), the mass showed slightly higher signal in

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13

Tago, Chiaki, and Noriyuki Yanagita. "Cochlear and Renal Pathology in the Autoimmune Strain Mouse." Annals of Otology, Rhinology & Laryngology 101, no. 10_suppl (1992): 87–91. http://dx.doi.org/10.1177/0003489492101s1018.

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This study was designed to investigate the role of immunologic mechanisms in sensorineural hearing disorders and the relationship between inner ear and renal disorders. Autoimmune strain (NZB/kl) mice, in which autoimmune disease can be spontaneously induced, were used in this study. The mice were tested for acoustic brain stem responses, cochlear and renal disorders, and circulating immune complex serology by means of enzyme immunoassay. Pathologic studies consisted of observation of tissue changes following hematoxylin and eosin staining and indirect immunofluorescence staining under light m

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14

DUBEKOVA, S. "WINTER WHEAT RESISTANCE TO YELLOW RUST IN SOUTHEAST KAZAKHSTAN." SABRAO Journal of Breeding and Genetics 55, no. 6 (2023): 1910–19. http://dx.doi.org/10.54910/sabrao2023.55.6.5.

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Wheat yellow (stripe) rust (Puccinia striiformis f. sp. tritici) is a dominant type of winter wheat disease. Developing new, highly productive varieties with increased immunological indicators helps to minimize the threat of rust spread. The progressive study searched the sources of resistance to the Pst populations and determined the effectiveness of Yr genes in Southeast Kazakhstan. Immunological studies ensued during 2018–2022 at the Kazakh Research Institute of Agriculture and Plant growing, Almaty, Kazakhstan. Wheat’s 23 isogenic lines and 193 winter wheat genotypes attained evaluation fo

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15

Viridiana, Altamirano Paredes, Aurora Macín Cabrera Susana, Estela Castrillón Rivera Laura, Ismael Castañeda Sánchez Jorge, and de Jesús Juárez Sandoval José. "Interleukin-13 Assessment in Gingival Crevicular Fluid of Patients with Periodontal Disease." RA JOURNALS OF APPLIED RESEARCH 11, no. 03 (2025): 184–89. https://doi.org/10.5281/zenodo.15062808.

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ABSTRACT: The present study holds significant potential to assess interleukin 13 (IL-13) levels in Crevicular Gingival Fluid (CGF) as a potential immunological marker for bone remodeling in subjects with periodontal disease. Thirty-nine subjects were included, with 19 diagnosed with gingivitis and 20 with periodontitis. The latter were classified following the new classification of periodontal and peri-implant diseases in stages I (6%), II (6%), III (6%), and IV (2%). Crevicular gingival fluid was meticulously collected with filter paper strips. Volume quantification was obtained with Periotro

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16

Aranha, Michelle P., Thomas A. Penfound, Jay A. Spencer, et al. "Structure-based group A streptococcal vaccine design: Helical wheel homology predicts antibody cross-reactivity among streptococcal M protein–derived peptides." Journal of Biological Chemistry 295, no. 12 (2020): 3826–36. http://dx.doi.org/10.1074/jbc.ra119.011258.

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Group A streptococcus (Strep A) surface M protein, an α-helical coiled-coil dimer, is a vaccine target and a major determinant of streptococcal virulence. The sequence-variable N-terminal region of the M protein defines the M type and also contains epitopes that promote opsonophagocytic killing of streptococci. Recent reports have reported considerable cross-reactivity among different M types, suggesting the prospect of identifying cross-protective epitopes that would constitute a broadly protective multivalent vaccine against Strep A isolates. Here, we have used a combination of immunological

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17

Helguera, Marcelo, Fernando Bravo-Almonacid, Ken Kobayashi, Pablo D. Rabinowicz, Vilma Conci, and Alejandro Mentaberry. "Immunological Detection of a GarV-Type Virus in Argentine Garlic Cultivars." Plant Disease 81, no. 9 (1997): 1005–10. http://dx.doi.org/10.1094/pdis.1997.81.9.1005.

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A cDNA library representing the genomes of several garlic viruses was generated using a viral RNA mixture as template. Analysis of randomly selected clones allowed the identification of different viral genomic sequences. On the basis of amino acid and nucleotide sequence comparisons, one of them was assigned to garlic virus A (GarV-A), a novel flexuous, rod-shaped virus recently reported in Japan. The coat protein (CP) of this virus was expressed in Escherichia coli cells and used as immunogen to produce polyclonal antibodies. The expression protein was recognized by an antiserum detecting gar

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18

Tarasenko, S. V., N. V. Muravyev, A. A. Arutyunyan, E. V. Ippolitov, and V. N. Tsarev. "Evaluation of clinical, microbiological and immunological effectiveness of a collagen sponge with antimicrobial and immunotropic properties." Parodontologiya 26, no. 4 (2022): 335–42. http://dx.doi.org/10.33925/1683-3759-2021-26-4-335-342.

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Relevance. The etiology of alveolitis distinguishes infectious and traumatic factors, which determine the relevant drugs for preventing the inflammatory process after tooth extraction and local treatment of the developed alveolitis. Purpose – the study aimed to evaluate the effectiveness of a collagen sponge containing lincomycin hydrochloride compared to the traditional method using iodoform packing strips based on clinical, microbiological and immunological research methods.Materials and methods. The study examined and surgically treated 75 patients with jaw alveolitis (K10.3), including tho

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19

Kumar, K. Antony, and Aruna R. "Intelligent Early Diagnosis System against Strep Throat Infection Using Deep Neural Networks." International Journal on Recent and Innovation Trends in Computing and Communication 11, no. 5 (2023): 01–11. http://dx.doi.org/10.17762/ijritcc.v11i5.6519.

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The most frequent bacterial pathogen causing acute pharyngitis is Group-A hemolytic Streptococcus (GAS), and sore throat is the second most frequent acute infection. The immunological reaction to group A Streptococcus-induced pharyngitis results in Acute Rheumatic Fever (ARF). A genetically vulnerable host for ARF is a streptococcal infection. ARF, which can affect various organs and cause irreparable valve damage and heart failure, is the antecedent to Rheumatic Heart Disease (RHD). RHD, in many countries is Cardiovascular Disease (CVD) refers to a range of conditions that affect the heart an

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20

Werneck, Antonio Luiz dos Santos, Luiz Carlos do Amaral Gurgel, Laurinda Moura de Mello, and Gabriella Queiroz de Albuquerque. "Sudden sensorineural hearing loss: a case report supporting the immunologic theory." Arquivos de Neuro-Psiquiatria 61, no. 4 (2003): 1018–22. http://dx.doi.org/10.1590/s0004-282x2003000600025.

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Sudden sensorineural hearing loss (SSNHL) is one of the autoimmune diseases of the inner ear (AIED), which is characterized by a hearing loss of above 30 decibels in at least three contiguous audiometric frequencies over a time course of 72 hours or shorter. Its cause can be found in only 10% to 15% of patients. Histopathologic findings have reported retrograde neuronal degeneration and atrophy of Corti's organ and of the vascular stria. This paper describes a case of a middle-aged female patient undergoing a treatment for hyperthyroidism who developed bilateral SSNHL. The patient was treated

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21

Mogensen, C. E., G. C. Viberti, E. Peheim, et al. "Multicenter Evaluation of the Micral-Test II Test Strip, an Immunologic Rapid Test for the Detection of Microalbuminuria." Diabetes Care 20, no. 11 (1997): 1642–46. http://dx.doi.org/10.2337/diacare.20.11.1642.

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22

Kleymann, G., C. Ostermeier, K. Heitmann, W. Haase, and H. Michel. "Use of antibody fragments (Fv) in immunocytochemistry." Journal of Histochemistry & Cytochemistry 43, no. 6 (1995): 607–14. http://dx.doi.org/10.1177/43.6.7769231.

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We developed a novel antibody fragment (Fv) technique for localization and determination of the surface topology of membrane protein complexes by immunogold electron microscopy. Several hybridoma cell lines producing murine monoclonal antibodies (MAbs) raised against bacterial membrane proteins were established. The cDNAs coding for the variable domains of the MAbs were cloned and expressed in Escherichia coli. The engineered Fv fragments served as trifunctional adapter molecules. The Fv fragment binds to the epitope of the membrane protein. The Strep tag fused to the VH chain was used for one

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23

Cohen, R. E., G. S. Bedi, M. E. Neiders, and B. Noble. "Induction of Type 2 Salivary Cystatin in Immunological and Chemical Kidney Injury." Critical Reviews in Oral Biology & Medicine 4, no. 3 (1993): 553–63. http://dx.doi.org/10.1177/10454411930040034201.

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We have previously reported that isoproterenol induces type 2 salivary cystatin in both submandibular glands and kidney tubule cells of rats but not in any other organs examined. In the present study, we investigated whether this salivary protein is induced in other conditions that show kidney tubule injury. Immunocytochemistry, using a monospecific antiserum to this cystatin, revealed specific staining within the proximal tubule epithelium of the cortex as well as in the inner and outer stripe of the medulla of immunologically and chemically injured rats. Cystatin could not be detected in kid

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24

Väänänen, H. K., E. K. Karhukorpi, K. Sundquist, et al. "Evidence for the presence of a proton pump of the vacuolar H(+)-ATPase type in the ruffled borders of osteoclasts." Journal of Cell Biology 111, no. 3 (1990): 1305–11. http://dx.doi.org/10.1083/jcb.111.3.1305.

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Microsomal membrane vesicles prepared either from chicken medullary bone or isolated osteoclasts were shown to have ATP-dependent H(+)-transport activity. This activity was N-ethylmaleimide-sensitive but resistant to oligomycin and orthovanadate, suggesting a vacuolar-type ATPase. Furthermore, immunological cross-reactivity of 60- and 70-kD osteoclast membrane antigens with Neurospora crassa vacuolar ATPase was observed when analyzed by immunoblotting. Same antibodies labeled only osteoclasts in chicken and rat bone in immunohistochemistry. Immunoelectronmicroscopy localized these antigens in

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25

Tomoda, Koichi, Toshio Yamawaki, Toshio Yamashita, Yuko Suzuka, and Tadami Kumazawa. "Alterations of Charge Barrier in the Inner Ear following Immune Reactions." Annals of Otology, Rhinology & Laryngology 101, no. 10_suppl (1992): 63–66. http://dx.doi.org/10.1177/0003489492101s1013.

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We investigated electron microscopically the changes of anionic sites of a charge barrier in the capillary basement membrane of the stria vascularis and endolymphatic sac following inner ear immune reactions. Hartley guinea pigs were immunized with bovine type II collagen, keyhole limpet hemocyanin, or horseradish peroxidase, with boosted and challenged antigens through the stylomastoid foramen. Animals were killed painlessly from several days up to 56 days after the antigen challenge. Polyethylenimine was used as a cationic tracer in order to observe the localization of anionic sites of the c

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26

Bansal, Radhika, Paschalis Vergidis, Pritish Tosh, et al. "Vaccine titers in lymphoma patients receiving chimeric antigen receptor T-cell therapy." Journal of Clinical Oncology 39, no. 15_suppl (2021): 7555. http://dx.doi.org/10.1200/jco.2021.39.15_suppl.7555.

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7555 Background: While CAR-T therapy is not myelo-ablative, patients with aggressive lymphoma treated with CD19 chimeric antigen receptor T cell therapy (CAR-T) are lymphodepleted and have prolonged B cell aplasia. The impact of CAR-T on immunologic protection from vaccine-preventable diseases (and thus the need to revaccinate) is not known. We report the vaccine titers of patients treated with axicabtagene ciloleucel (axi-cel) at Mayo Clinic. Methods: Retrospective chart review of adult lymphoma patients who received axi-cel from 9/2018 to 9/2020 for anti-viral and anti-bacterial titers prior

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27

Sakagami, Masafumi, Schunichi Tomiyama, Masanori Umemoto, Keuiro Fukazawa, and Takeshi Kubo. "Vascular Permeability of the Stria Vascularis and Morphology of the Endolymphatic Sac in two Types of Experimental Endolymphatic Hydrops." Annals of Otology, Rhinology & Laryngology 104, no. 3 (1995): 210–17. http://dx.doi.org/10.1177/000348949510400306.

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We produced two kinds of experimental endolymphatic hydrops. One was induced by obliteration of the endolymphatic sac (ES), and the other was induced by both systemic keyhole limpet hemocyanin immunization and secondary keyhole limpet hemocyanin challenge into the ES. The vascular permeability of the stria vascularis in acute and chronic phases was compared between both models by light and electron microscopy using the tracer method of horseradish peroxidase. Both models showed that the number of strial capillaries showing horseradish peroxidase leakage was significantly higher in the acute ph

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28

DONATSKY, O. "AN IMMUNOELECTROPHORETIC ANALYSIS OF THE STREP. SANGUIS AND ADULT HUMAN ORAL MUCOSA ANTIGEN EXTRACTS USED FOR IMMUNOLOGICAL INVESTIGATIONS OF RECURRENT APHTHOUS STOMATITIS." Acta Pathologica Microbiologica Scandinavica Section C Immunology 88C, no. 1-6 (2009): 219–26. http://dx.doi.org/10.1111/j.1699-0463.1980.tb00097.x.

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29

Ando, Motonori, and Shunji Takeuchi. "Immunological identification of an inward rectifier K + channel (Kir4.1) in the intermediate cell (melanocyte) of the cochlear stria vascularis of gerbils and rats." Cell and Tissue Research 298, no. 1 (1999): 179–83. http://dx.doi.org/10.1007/s004419900066.

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30

Vasquez-Gutierrez, Ubilfrido, Gustavo Alberto Frías-Treviño, Luis Alberto Aguirre-Úribe, Alberto Flores-Olivas, and Juan Carlos Delgado-Ortiz. "Severity of Tomato brown rugose fruit virus in tomato (Solanum lycopersicum L.) from a region of Coahuila, México." International Journal of Horticulture, Agriculture and Food science 7, no. 2 (2023): 1–6. http://dx.doi.org/10.22161/ijhaf.7.2.1.

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The purpose of this research was to describe the Tomato brown rugose fruit virus, from three isolates collected in the field and also to use a diagrammatic scale of severity for its evaluation. The isolation was carried out with the collection of 200 ha-1 leaflets, according to a statistical method, from commercial greenhouses. Agdia® immunological strips were used to confirm the symptoms and the virus was identified by RT-PCR. A completely randomized experimental design was established in tomato plants var. Río Grande®, with three treatments or isolates and five repetitions: Blindom F1® tissu

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31

Laubscher, B., G. van Melle, N. Dreyfuss, and H. de Crousaz. "Evaluation of a new immunologic test kit for rapid detection of group A streptococci, the Abbott Testpack Strep A plus." Journal of clinical microbiology 33, no. 1 (1995): 260–61. http://dx.doi.org/10.1128/jcm.33.1.260-261.1995.

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32

Kasetsirikul, Surasak, Kimberley Clack, Muhammad J. A. Shiddiky, and Nam-Trung Nguyen. "Rapid, Simple and Inexpensive Fabrication of Paper-Based Analytical Devices by Parafilm® Hot Pressing." Micromachines 13, no. 1 (2021): 48. http://dx.doi.org/10.3390/mi13010048.

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Paper-based analytical devices have been substantially developed in recent decades. Many fabrication techniques for paper-based analytical devices have been demonstrated and reported. Herein, we report a relatively rapid, simple, and inexpensive method for fabricating paper-based analytical devices using parafilm hot pressing. We studied and optimized the effect of the key fabrication parameters, namely pressure, temperature, and pressing time. We discerned the optimal conditions, including a pressure of 3.8 MPa, temperature of 80 °C, and 3 min of pressing time, with the smallest hydrophobic b

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Hocquette, J. F., F. Bornes, M. Balage, P. Ferre, J. Grizard, and M. Vermorel. "Glucose-transporter (GLUT4) protein content in oxidative and glycolytic skeletal muscles from calf and goat." Biochemical Journal 305, no. 2 (1995): 465–70. http://dx.doi.org/10.1042/bj3050465.

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It is well accepted that skeletal muscle is a major glucose-utilizing tissue and that insulin is able to stimulate in vivo glucose utilization in ruminants as in monogastrics. In order to determine precisely how glucose uptake is controlled in various ruminant muscles, particularly by insulin, this study was designed to investigate in vitro glucose transport and insulin-regulatable glucose-transporter protein (GLUT4) in muscle from calf and goat. Our data demonstrate that glucose transport is the rate-limiting step for glucose uptake in bovine fibre strips, as in rat muscle. Insulin increases

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Okeke, O. A., S. P. Nnanwuba, C. O. Ishar, et al. "Malaria Parasite Density and Associated Risk Factors in Pregnant Women Attending Antenatal Care in a Tertiary Healthcare Facility in Awka." Sahel Journal of Life Sciences FUDMA 3, no. 2 (2025): 114–21. https://doi.org/10.33003/sajols-2025-0302-14.

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Malaria remains a leading cause of morbidity and mortality in sub-Saharan Africa, particularly among pregnant women, who are at increased risk due to immunological and physiological changes. This cross-sectional hospital-based study was carried out to investigate malaria parasite density and associated risk factors among pregnant women attending antenatal clinic at Chukwuemeka Odumegwu Ojukwu University Teaching Hospital, Anambra State, Nigeria. A total of 101 venous blood samples were collected and analysed for malaria parasite using microscopy, while urine samples were analyzed for biochemic

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Loza, Samantha, Brandon Tallman, Keith Hanson, and Shane Rainey. "A 15-year-old with chest pain: An unexpected etiology." SAGE Open Medical Case Reports 10 (January 2022): 2050313X2110690. http://dx.doi.org/10.1177/2050313x211069026.

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A 15-year-old female with no significant past medical history presented to the emergency department with 1 day of substernal and pleuritic chest pain, chills, cough, and hematuria. She also had swelling of the face and ankles that resolved by presentation. She was found to have elevated troponin and brain natriuretic peptide during initial workup. Electrocardiogram was normal, but there were significant pleural effusions on chest x-ray. She was strep positive and had blood pressure up to 150/90, prompting admission for cardiac monitoring and cardiology consultation. Blood pressure decreased do

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El-Naggar, Randa Ezz El-Din. "Hypoglycemic and Immunostimulating Activities of Exopolysaccharides from Ganoderma sp. Mushroom in Streptozotocin-Induced Diabetes in Rats." South Asian Journal of Experimental Biology 6, no. 3 (2016): 105–11. http://dx.doi.org/10.38150/sajeb.6(3).p105-111.

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The incidence of diabetes mellitus is increasing dramatically worldwide. Different studies support an association between diabetes and decreased function of the immune system. On the other hand, Ganoderma mushroom, and its bioactive compounds, is widely used and recommended by physicians for its supporting effects on immune system. Therefore, this work aimed to test the hypoglycemic and immunomodulating activities of Ganoderma sp. exopolysaccharides (EPS) in streptozotocin (STZ)-induced diabetes in rats. Animals were divided into control, diabetic and Ganoderma EPS-treated (10, 50 or 100 mg/kg

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Aljuboori, Ismael W., and Maha Sh Mahmood. "The Effects of Salvia officinalis Gel as an Adjunct to Scaling and Root Planning in Patients with Periodontitis (Clinical and Immunological Study)." International Journal of Drug Delivery Technology 10, no. 02 (2020): 232–37. http://dx.doi.org/10.25258/ijddt.10.2.8.

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Background: Phytotherapy is the usage of herbal species with medicinal properties for the management of various diseases. Gingivitis and periodontitis are diseases that involve the role of both the bacteria and the host immune response. Over the years, various researches have shown the importance of herbal products in the management of periodontal diseases. Aims of the study: To evaluate the efficacy of locally applied Salvia officinalis gel as adjunctive in the treatment of chronic periodontitis. Subjects and methods: Fourteen patients (10 males and 4 females) with chronic periodontitis were

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Nemeth, Nicole M., Angela M. Bosco-Lauth, Lisa M. Williams, Richard A. Bowen, and Justin D. Brown. "West Nile Virus Infection in Ruffed Grouse (Bonasa umbellus): Experimental Infection and Protective Effects of Vaccination." Veterinary Pathology 54, no. 6 (2017): 901–11. http://dx.doi.org/10.1177/0300985817717770.

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Ruffed grouse ( Bonasa umbellus) population numbers in Pennsylvania dramatically declined during the early 2000s and have subsequently remained depressed throughout much of the state. While this decline has been temporally associated with the presence of West Nile virus (WNV), lack of information on the WNV susceptibility of this popular game bird species has limited the ability to interpret the potential impacts of WNV. To address this knowledge gap, virologic, immunologic, pathologic, and clinical responses as well as protective effects of vaccination following experimental WNV inoculation i

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Buckner, C. K., R. I. Fishleder, R. Conklin, J. A. Will, O. Doran, and F. M. Graziano. "Pharmacologic modulation of the influence of the epithelium on immunologic- and nonimmunologic-induced histamine release and contraction in guinea pig superfused tracheal strips." Journal of Pharmacology and Experimental Therapeutics 264, no. 2 (1993): 717–25. https://doi.org/10.1016/s0022-3565(25)10199-7.

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Nestorovic, Branimir, Suzana Laban-Nestorovic, Veselinka Paripovic, and Katarina Milosevic. "Value of rapid test for identification of beta hemolytic Streptococcus antigens in children with Streptococcal pharyngitis." Srpski arhiv za celokupno lekarstvo 132, suppl. 1 (2004): 39–41. http://dx.doi.org/10.2298/sarh04s1039n.

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Beta-hemolytic group A streptococcus (Streptococcus pyogenes) is the most common bacterial agent associated with the upper respiratory tract infections in humans. The most frequently group A streptococcus-associated disease is pharyngitis. Males and females are equally affected by group A streptococcus. There is seasonal increase in the prevalence of group A streptococcus-associated pharyngitis. Streptococcal pharyngitis is most prevalent in winter and early spring with higher incidence of disease observed in crowded population such as school children. Early diagnosis and treatment of group A

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Sulahian, Annie, Yves Jean François Garin, Arezki Izri, et al. "Development and Evaluation of a Western Blot Kit for Diagnosis of Schistosomiasis." Clinical Diagnostic Laboratory Immunology 12, no. 4 (2005): 548–51. http://dx.doi.org/10.1128/cdli.12.4.548-551.2005.

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ABSTRACT We evaluated the performance of Western blot (WB) analysis using commercially available antigen strips and compared the results with those of indirect hemagglutination (IHA) and indirect immunofluorescence (IFAT) for the serodiagnosis of human schistosomiasis. The antigen preparation was a crude extract of Schistosoma mansoni. The WB profile characteristics of schistosomiasis were characterized by comparing the results for 58 serum samples from patients with parasitologically proven S. mansoni (n = 12) and S. haematobium (n = 46) infections and 37 individuals with probable cases of sc

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Marangoni, Antonella, Claudio Foschi, Maria Grazia Capretti, et al. "Contribution of a Comparative Western Blot Method to Early Postnatal Diagnosis of Congenital Syphilis." Clinical and Vaccine Immunology 23, no. 5 (2016): 410–16. http://dx.doi.org/10.1128/cvi.00032-16.

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ABSTRACTSerology has a pivotal role in the diagnosis of congenital syphilis (CS), but problems arise because of the passive transfer of IgG antibodies across the placenta. The aim of this study was to assess the diagnostic value of a comparative Western blot (WB) method finalized to match the IgG immunological profiles of mothers and their own babies at birth in order to differentiate between passively transmitted maternal antibodies and antibodies synthesized by the infants againstTreponema pallidum. Thirty infants born to mothers with unknown or inadequate treatment for syphilis were entered

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De Boer, Florentine, Sanja Kezic, and Henk Van der Molen. "P-310 BIOMARKERS OF UV-RADIATION IN INDOOR AND OUTDOOR WORKERS." Occupational Medicine 74, Supplement_1 (2024): 0. http://dx.doi.org/10.1093/occmed/kqae023.0917.

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Abstract Introduction Outdoor workers (OW) are exposed to higher doses of ultraviolet radiation (UVR) compared to indoor workers (IW), which increases their risk of developing skin cancer. Methods and Results Tape stripping of the stratum corneum (SC) is a non-invasive tool for identification of biomarkers that can aid in the early detection and prevention of chronic UV-related skin damage in the occupational setting. SC tape strips were collected from two different skin sites: one on less exposed skin behind the ear (E) and another from the more exposed forehead (F) skin. The study comprised

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Sun, Ning, Chenchen Li, Yue Teng, Yuxia Deng, and Lin Shi. "Pan-Cancer Analysis on the Oncogenic Role of Programmed Cell Death 10." Journal of Oncology 2022 (October 13, 2022): 1–11. http://dx.doi.org/10.1155/2022/1242658.

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Purpose. Programmed cell death factor 10 (PDCD10) is associated with intercellular junction, cytoskeleton organization, cell proliferation, apoptosis, exocytosis, and angiogenesis. However, the role of PDCD10 in human cancer is unclear. This study aims to explore the role of PDCD10 in various tumors and its possible mechanism through bioinformatics analysis. Methods. We verified the expression of the PDCD10 gene based on data from the ONCOMINE, TIMER2.0, and TISDB databases. The correlation of PDCD10 with prognosis of patients with different tumors was analyzed using data from the GEPIA2 datab

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Sreebny, L. M., and W. X. Zhu. "The Use of Whole Saliva in the Differential Diagnosis of Sjögren's Syndrome." Advances in Dental Research 10, no. 1 (1996): 17–24. http://dx.doi.org/10.1177/08959374960100010201.

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Sjögren's Syndrome (SS) is a chronic, multisystem. autoimmune disorder. It is characterized by (1) generalized exocrine gland dysfunction, (2) serologic abnormalities, and (3) organ-system changes. Oral changes are a prominent feature of this disease. Among these are xerostomia and hypofunction of the salivary glands. Given the intimate relationship between SS and the salivary glands, it is reasonable to postulate that whole saliva (WS) contains the stigmata associated with the presence of this disease. But few studies have been conducted on this secretion. Indeed, WS has largely been neglecte

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Sheshegova, Т. К., and I. N. Shchennikova. "SOURCES OF BARLEY RESISTANCE TO HELMINTHOSPORIOTIC DISEASES AND THEIR USE IN THE NORTH-EAST FARC." Bulletin of NSAU (Novosibirsk State Agrarian University), no. 2 (July 15, 2020): 76–83. http://dx.doi.org/10.31677/2072-6724-2020-55-2-76-83.

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The paper explores 70 samples of spring barley from the collection of WIR under conditions of rather rigid natural infectious helminthosporioses (striped, reticulated and dark brown spotting) in FARC of the North-East in 2018-2019. The spotting was investigated 4-5 times during plants ontogenesis every 10-12 days since the phase 29 on the Cadox scale. The methodology suggested by O.S. Afanasenko (2005) was used to assess the diseases. The nature of vegetative-microbial interactions was evaluated by SCDP (square under the curve of disease progress) and IS (stability index) indicators. During im

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Bansal, Radhika, Paschalis Vergidis, Pritish K. Tosh, et al. "Vaccine Titers in Lymphoma Patients Receiving Chimeric Antigen Receptor T Cell Therapy." Blood 138, Supplement 1 (2021): 3857. http://dx.doi.org/10.1182/blood-2021-153500.

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Abstract Background: While CD19 chimeric antigen receptor T cell therapy (CAR-T) therapy does not use myeloablative chemotherapy, patients with aggressive lymphoma treated with CAR-T are immunosuppressed from lymphodepletion chemotherapy, and prolonged B cell aplasia and hypogammaglobulinemia from CAR-T. The impact of CAR-T on immunologic protection from vaccine-preventable diseases (and thus the need to revaccinate) has not been formally studied. We report the vaccine titers of patients treated with CAR-T at Mayo Clinic, Rochester. Methods: We conducted a retrospective chart review of patient

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Sharmin, S., S. Ahmed, A. Abu Saleh, F. Rahman, MR Choudhury, and MM Hassan. "Association of Immunofluorescence pattern of Antinuclear Antibody with Specific Autoantibodies in the Bangladeshi Population." Bangladesh Medical Research Council Bulletin 40, no. 2 (2015): 74–78. http://dx.doi.org/10.3329/bmrcb.v40i2.25225.

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Antinuclear antibody (ANA) is useful in the diagnosis of connective tissue disorder (CTD). Association of specific autoantibodies with the immunofluorescence pattern of ANA in CTD, noted in western literature has been considered as reference in all over the world. However, in Bangladesh no such research work or data correlating the autoantibodies and their ANA patterns is found. Objective of the study was to identify an association between immunofluorescence patterns of antinuclear antibody on HEp-2 cell and more specific antinuclear reactivities (e.g. anti-dsDNA and anti-extractable nuclear a

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Bossio, Sabrina, Laura De Stefano, Mariavaleria Pellicanò, et al. "Differentially Expressed Protein Patterns in Chronic Lymphocytic Leukemia (CLL) after Thymosin beta4 (Tb4) and Lenalidomide (Len) Treatment: Two-Dimensional Gel Electrophoresis (2DE) Analysis." Blood 126, no. 23 (2015): 1729. http://dx.doi.org/10.1182/blood.v126.23.1729.1729.

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Abstract Proteomic approaches are commonly secondary to genetic studies but are essential in the multi-disciplinary field of hematological research. As opposed to mRNA microarray data, proteomics provides a better understanding of which proteins are actually expressed, although the identification of specific proteins remains challenging (Unwin et al Blood Rev 2014; Boyd J Proteomics 2010). In neoplastic hematology such as CLL, protein studies have contributed to the elucidation of disease mechanisms, defined prognostic or therapeutic biomarkers (Boyd J Proteomics 2010). In this study we used p

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Sharmin, Sadia, Sharmeen Ahmed, Humayun Sattar, Md Ruhul Amin Miah, Minhaz Rahim Chowdhury, and M. Masudul Hassan. "Immunofluorescence pattern of antinuclear antibody and its association with autoantibody profile in systemic lupus erythematosus." Bangabandhu Sheikh Mujib Medical University Journal 6, no. 2 (2016): 141. http://dx.doi.org/10.3329/bsmmuj.v6i2.29130.

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<p><strong>Background:</strong> Antinuclear antibody (ANA) is useful in the diagnosis of systemic lupus erythematosus (SLE). Association of specific autoantibodies with the immunofluorescence pattern of ANA in SLE as noted in Western literature has been taken as reference in all over the world. However, in Bangladesh such research work or data correlating the autoantibodies and their ANA patterns is inadequate. <strong></strong></p><p><strong>Objective:</strong> To identify an association between immunofluorescence patterns of antinuclear a

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