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1
Sudhir, Bhatia. "LOSS OF AN ABUNDANT QUANTITY OF RIBONUCLEIC ACID DURING MINI COLUMN ISOLATION METHOD." Biotechnologia Acta 16, no. 3 (June 30, 2023): 65–69. http://dx.doi.org/10.15407/biotech16.03.065.
Повний текст джерелаАнотація:
Aim: The Isolation of nucleic acid is an important step for conducting different molecular assays in many laboratories around the world. It is also a common practice that user is isolating the ribonucleic acid (RNA) from the samples with mini column once and throwing away the supernatant. This makes isolated RNA as limiting factor in many studies as this issue has not been addressed in literature. Therefore, we decided to conduct whether it is a loss of ribonucleic acid during the mini column isolation method. Method: In this research, the mini column isolations were done with different samples of human tissues from placenta and umbilical cords and subsequent isolations of supernatants. Yields and successful isolations of RNA were assessed with spectrometric instrument and real time PCR machine. Results: It was found that there is loss of abundant quantity of RNA during the subsequent isolations. The amount measured with UV spectrometer indicates that some times 2nd and 3rd isolation have more RNA than the first isolation. Realtime PCR for house keeping gene beta actin shows that presence of RNA can be seen up to 6 isolation cycles from supernatants. Conclusion: There is loss of RNA in subsequent isolations with mini column method, therefore it is possible to isolate more RNA from subsequent supernatant isolations. User should do the multiple isolations to get higher yield of RNA.
2
Kocur, Sylwia, and Mirosława Noppenberg. "Izolacja pacjentów hospitalizowanych w szpitalu specjalistycznym." Journal of Life and Medical Sciences 1(37)/2024 (October 16, 2024): 31–38. https://doi.org/10.5281/zenodo.14014917.
Повний текст джерелаАнотація:
Isolation is a regular part of any infection prevention program. The main purpose of isolationis to create a barrier to prevent the spread of microorganisms from an infected or colonized per-son to other patients, hospital visitors and health care workers. The use of protective measuresand isolation are essential to control the spread of epidemic and endemic microorganisms. Theeffectiveness of isolation depends on the speed with which infectious patient are identified andappropriate precautions are taken.The purpose of this study was to present the implementation of the procedure for preventingthe spread of nosocomial infections in a specialty hospital.The research method used was documentoscopy, classified as a quantitative research method.The technique used was a retrospective analysis of Hospital Infection Control Team documen-tation. The documentation of the Hospital Infection Control Team was analyzed - monitoringof isolation rooms in operation covering the period from January to May 2024. The analysisincluded 171 isolation cases out of 6667 treated patients at the Józef Dietl Specialist Hospital inKrakow.The largest number of patients was isolated in the Second Department of Internal Medicineand Gastroenterology - 70 patients. In the Orthopedics and Rehabilitation department, therewere no indications of isolation. The highest percentage of patients isolated in relation to thetotal number of hospitalized patients in the analyzed period occurred in the Second Departmentof Internal Medicine and Gastroenterology and amounted to 8.13%. Most isolations were con-ducted due to influenza A virus. There were also repeated isolations of the same patients.Infectious diseases are most often transmitted by contact and airborne droplet routes. Theaverage isolation time was the longest for protective isolation and the shortest for air-dust isola-tion.
3
TADA, HIDEYUKI. "Seismic Isolation for Buildings. Seismic Isolation and Elastomeric Isolator." NIPPON GOMU KYOKAISHI 70, no. 8 (1997): 421–25. http://dx.doi.org/10.2324/gomu.70.421.
Повний текст джерела
4
Muwafaq Azeez, Iman, and Mohammed Nather Maa'roof. "Isolation and Identification of some Bacterial Species From Cervix Infected Women in Salahuddin Governorate." Tikrit Journal of Pharmaceutical Sciences 13, no. 1 (April 26, 2023): 44–53. http://dx.doi.org/10.25130/tjphs.2018.13.1.7.44.53.
Повний текст джерелаАнотація:
This study was carried out for isolating some pathogenic types of positive and negative bacteria for Gram stain, which cause the infection of the vaginitis in infected women and its diagnosis by routine laboratory methods and confirm the diagnosis using Vitek2compact system by collecting fifty samples of the swab of the cervix in the period between October 2015 till April 2016 from the in patients and out patients women in Salahuddin Hospital for the age groups between (14-50) years.The results of isolation show the percentage of bacterial growth was 78%, it reached samples in 78%.The results of the diagnosis of routine diagnostic tests, which were confirmed by using VITEK comparte2 system that there was a diversity of bacterial species including bacterial species from the source of isolation of vaginal infection, where the Escherichia coli bacteria had the highest isolation rate of isolation in 22%, followed by other bacterial species with different isolations. It was observed that there was a variation in the isolation rates and bacterial infections species of the vagina where bacterial isolates were isolated Proteus mirabilis isolates in 20.5% and as Klebsiella pneumonia showed isolations in 10.3%. For Salmonicida Aeromonas and Staphylococcus hominis Bacteria, the isolates was in 7.7%, While Pseudomonas luteola, Pantoes spp, Pseudomonas oryzihabitans, Ochrobactrum anthropic, Sphingomonas paucimbilis bacteria, isolations were isolated in 5.1 %.All isolates were characterized by their high resistance to ten different types of antibiotics, which represent: Gentamicin, Oxacillin, Tetracyclin, Cefotaxme, Amplicillin Novabiocin, Chloramphenicol, Rifampin, Trimethoprim and Azithromycin. The bacterial isolates gave absolute resistance to 100% of the majority of antibiotics used in our current study, while most isolates were sensitive to antagonists Gentamicin
5
Waa'd M. Raoof, Akeel H. Al-Assie, and Shaimaa Monshed Morshed. "Using RAPD markers for Detection the genetic diversity of E. coli isolates from Kirkuk hospitals." Tikrit Journal of Pure Science 23, no. 5 (January 18, 2023): 65–72. http://dx.doi.org/10.25130/tjps.v23i5.589.
Повний текст джерелаАнотація:
300 samples from different infection sourcel for a patients and revisers in Azadi hospital, Kirkuk generic hospital, and children hospital, from various ages for both genders, to isolate Escherichia coli bacteria and diagnosis it, and study the genetic variety which distribution to clinical samples by (140) urine samples, (45) diarrhea samples, (40) wound swab, (40) burn swab, and (35) from otitis media infection.
 The growth isolation on the different cultural media was diagnosed from the appearance, microscopical, and cultural characteristics. The results the diagnosis showed that (75) clinical isolation about (38.65%) refers to E. coli bacteria distributed by (9) isolation at ratio (31%) from the burn swab, (6) isolation at ratio (26.1) from wound swab, (4) isolation from otitis media infection (19%), (13) isolation from the stool about (40.6%), and (43) isolation (84.3%) from the urinary tract infection (UTI).
 The sensitivity of the clinical and ecological isolations was studied for nine antibiotics from different antibiotics, broadly. Where the highest resistance for the antibiotics in the clinical isolations to both (ampicillin and cephalexin) reaching in the rate of (97.3%) and (92%), respectively. While the lowest resistance rate for the clinical isolations was to the (Amikacin) in the rate of (17.3%).
 It has been selected 24 clinical isolations, and 24 ecological isolations from E. coli isolations depending on here resistance to many of antibiotics. To study the genetic variation for these isolations and the genetic markers it has been used a type of molecular markers which is depending on PCR technical it is: randomly Amplified polymorphic DNA (RAPD).
6
Xu, Fang Mei, Shi Hai Chen, and Peng Li. "Study on Seismic Response of the Structure Based on New-Style Isolation Bearings." Advanced Materials Research 1065-1069 (December 2014): 1503–7. http://dx.doi.org/10.4028/www.scientific.net/amr.1065-1069.1503.
Повний текст джерелаАнотація:
The new-style isolation bearings that can isolate the seismic effect in horizontal and vertical direction is presented in this paper. In order to study isolation of bearings, structure seismic isolating model is established based on new-style isolation bearings. Through the input of seismic excitation, acceleration peak and storey drift are regarded as basic discrimination factor for studying seismic response, and structure seismic isolating model is contrasted with structure seismic non-isolating model. The example shows that the new-style of isolation bearings not only can isolate in the horizontal direction, but also isolation ability is better in the vertical direction.
7
Dijk, Manon van, t. holt, tte Severin, Suzanne Polinder, and M. Vos. "The Daily Direct Costs of Isolating Patients Identified With Highly Resistant Microorganisms." Infection Control & Hospital Epidemiology 41, S1 (October 2020): s404. http://dx.doi.org/10.1017/ice.2020.1053.
Повний текст джерелаАнотація:
Background: Isolation precautions are recommended when caring for patients identified with highly resistant microorganisms (HRMOs). However, the direct costs of isolating patients are largely unknown. Therefore, we aimed to obtain detailed information on the daily direct costs associated with isolating patients identified with HRMO. Methods: This study was performed from November until December 2017 on a 12-bed surgical ward. This ward contained solely isolation rooms with an anteroom. The daily direct costs of isolation were based on three cost items: (1) additional personal protective equipment (PPE); measured by counting the consumption of empty packaging materials, (2) cleaning and disinfection of the isolation room; based on the costs of an outsourced cleaning company, and (3) additional workload for healthcare workers; based on literature and multiplied by the average gross hourly salary of nurses. A distinction was made between the costs for strict isolation, contact-plus isolation, and contact isolation. Results: During the study period, 26 patients were nursed in isolation because of HRMO carriage, resulting in a total of 304 isolation days (median 7 isolation days; range 1-44). Gloves were consumed the most and hair caps the least. The average daily direct costs of isolation were the least expensive for contact isolation, €28/$31, and the most expensive for strict isolation, €41/$47. Conclusions: By using a novel, easy method to estimate consumption of PPE, we conclude that the daily direct costs of isolating a patient, differs per type of isolation. Insight into the direct costs of isolation is of utmost importance when developing or revising policies.Funding: NoneDisclosures: None
8
Qu, Junhao, and Shumin Zhang. "Isolation Number of Transition Graphs." Mathematics 13, no. 1 (December 30, 2024): 116. https://doi.org/10.3390/math13010116.
Повний текст джерелаАнотація:
Let G=(V,E) be a graph and F be a family of graphs; a subset (S⊆V(G)) is said to be an F-isolating set if G[V(G)∖NG[S]] does not contain F as a subgraph for all F∈F. The F-isolation number of G is the minimum cardinality of an F-isolating set (S) of G, denoted by ι(G,F). When F={K1,k+1}, we use ιk(G) to define the F-isolation number (ι(G,F)). In particular, when k=0, we use the short form of ι(G) instead of ι0(G). A subset (S⊆V(G)) is called an isolating set if V(G)∖NG[S] is an independent set of G. The isolation number of G is the minimum cardinality of an isolating set, denoted by ι(G). In this paper, we mainly focus on research on the isolation number and F-isolation number of a B(G) graph, total graph and central graph of graph G.
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Bhatia, Sudhir, and Gudrun Baersch. "Rapid Isolation of Mononuclear Cells with High Yield from Minimal Blood Volumes: A Simplified and Robust Approach for Immunotherapeutic Applications." Medical Science and Discovery 10, no. 10 (October 18, 2023): 838–41. http://dx.doi.org/10.36472/msd.v10i10.1062.
Повний текст джерелаАнотація:
Objective: Currently, mononuclear cell (MNC) isolation is performed using density gradient methods, which are laborious and time-consuming, requiring a minimum of 10 ml of buffy coat or blood. This method is inadequate for isolating MNCs from small volumes, such as 100 µl. Therefore, we aimed to develop a straightforward, rapid, and cost-effective method for isolating MNCs from minute volumes of blood and buffy coat. Material and Methods: We utilized the MNC isolator to isolate MNCs from microvolumes, such as 100 µl and 500 µl of blood and buffy coat, by simple mixing and centrifugation. The isolated cells were cultured, and ligand-specific magnetic beads were employed to isolate CD4 and CD45 specific cells from the cultured cells. Results: In 15 experiments, the MNC isolator successfully isolated MNCs from small volumes of blood and buffy coat. The cell cultures were established and maintained for up to 3 years without contamination, with the cells remaining healthy. Successful isolations of CD4 and CD45 cells using magnetic beads were achieved, and the cells were successfully frozen and thawed. Conclusion: In this study, we have introduced a simple, cost-effective, and robust method for isolating MNCs from small volumes of buffy coat and blood, suitable for immunological applications and magnetic bead cell isolation. This method is user-friendly and can be adopted in cell culture laboratories worldwide, potentially opening new avenues for the development of novel antibodies, isolation of various cell populations using magnetic beads, and obtaining a large number of T-cells for immunotherapy development, such as CAR T-cell therapies. Keywords: mononuclear cell isolation, buffy coat, blood, CD4 positive cells, magnetic beads
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Wang, Xingxing, Yujie Zhang, Hongjun Ni, Shuaishuai Lv, Yu Zhu, Guosheng Yang, and Weidong Cai. "Research on UHV Isolating Switch Technology with Multi-source Action Signal." Journal of Physics: Conference Series 2137, no. 1 (December 1, 2021): 012036. http://dx.doi.org/10.1088/1742-6596/2137/1/012036.
Повний текст джерелаАнотація:
Abstract With the improvement of the voltage level of power transmission and transformation lines, the development of smart grids has highlighted a major demand, and relevant enterprises and scientific researchers in the UHV field at home and abroad have explored the research of water intelligent isolation switch equipment. This article explains the current problems of traditional substation isolation switches, and analyzes the advantages and disadvantages of UHV isolation switch technology and its application status through three aspects: isolation switch, isolation switch contact and isolation switch sensor. Studies have shown that the development and application of isolating switches with multi-source action signals, and further improving the proportion and accuracy of smart devices to replace manual labor are the development trends of UHV isolating switches.
11
Moťková, P., and J. Vytřasová. "Comparison of methods for isolating fungal DNA." Czech Journal of Food Sciences 29, Special Issue (January 4, 2012): S76—S85. http://dx.doi.org/10.17221/266/2011-cjfs.
Повний текст джерелаАнотація:
In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification before the proper DNA detection itself. For this purpose, classic methods of DNA extraction were compared and optimised while isolations using commercial kits were also done. The methods were evaluated from several perspectives, with focus especially laid on the isolated DNA not contain PCR inhibitors which would prevent DNA amplification, thus inhibiting the detection itself. For optimising the individual methods, collection strains of the genus Aspergillus were used. After the evaluation, two most suitable methods were selected and chosen for isolating potentially aflatoxigenic moulds taken from food samples. These methods were the commercially supplied kit for isolating DNA from plant leaves from Sigma and a classic method according Cenis in combination with the cell wall disruption by means of liquid nitrogen.
12
Duan, Shao Wei, Xiao Wei Tao, and Hai Kuan Liu. "The Isolation Effect Analysis of Base and Story Isolation System in Vertical Seismic Action." Advanced Materials Research 163-167 (December 2010): 3893–98. http://dx.doi.org/10.4028/www.scientific.net/amr.163-167.3893.
Повний текст джерелаАнотація:
Through establishing single material point model of base isolation system and two material points model of story isolation system in vertical earthquake, the isolation effect of base and story isolation system in vertical earthquake are studied. Results show that two kinds of isolation systems cannot effectively keep apart the vertical seismic action, and the effect is amplified on the contrary, especially the position of isolation layers in story structures affect the amplification effect. Compared with the base and story isolation structures, while using the same isolation device, the former is better than the latter in isolating vertical seismic action.
13
Schumacher, Jakob, Lisa Kühne, Sophia Brüssermann, Benjamin Geisler, and Sonja Jäckle. "COVID-19 isolation and quarantine orders in Berlin-Reinickendorf (Germany): How many, how long and to whom?" PLOS ONE 19, no. 3 (March 11, 2024): e0271848. http://dx.doi.org/10.1371/journal.pone.0271848.
Повний текст джерелаАнотація:
Isolating COVID-19 cases and quarantining their close contacts can prevent COVID-19 transmissions but also inflict harm. We analysed isolation and quarantine orders by the local public health agency in Berlin-Reinickendorf (Germany) and their dependence on the recommendations by the Robert Koch Institute, the national public health institute. Between 3 March 2020 and 18 December 2021 the local public health agency ordered 24 603 isolations (9.2 per 100 inhabitants) and 45 014 quarantines (17 per 100 inhabitants) in a population of 266 123. The mean contacts per case was 1.9. More days of quarantine per 100 inhabitants were ordered for children than for adults: 4.1 for children aged 0-6, 5.2 for children aged 7-17, 0.9 for adults aged 18-64 and 0.3 for senior citizens aged 65-110. The mean duration for isolation orders was 10.2 and for quarantine orders 8.2 days. We calculated a delay of 4 days between contact and quarantine order. 3484 contact persons were in quarantine when they developed an infection. This represents 8% of all individuals in quarantine and 14% of those in isolation. Our study quantifies isolation and quarantine orders, shows that children had been ordered to quarantine more than adults and that there were fewer school days lost to isolation or quarantine as compared to school closures. Our results indicate that the recommendations of the Robert Koch Institute had an influence on isolation and quarantine duration as well as contact identification and that the local public health agency was not able to provide rigorous contact tracing, as the mean number of contacts was lower than the mean number of contacts per person known from literature. Additionally, a considerable portion of the population underwent isolation or quarantine, with a notable number of cases emerging during the quarantine period.
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Kazakov, Maxim. "The study of isolation in the collectives of special-purpose railroad cars as an element of the sustainable development of social systems." E3S Web of Conferences 402 (2023): 06015. http://dx.doi.org/10.1051/e3sconf/202340206015.
Повний текст джерелаАнотація:
The article provides examples of labour social isolations. Multiple professions in which social isolation is present are compared, and differences are revealed both in the process of isolation itself and the influence of other psychogenic factors. The question was raised about the complexity of classifying labour social isolation. The definition of social exclusion is given. Teams that face social isolation were considered. The main differences were identified, both in psychogenic factors and in the isolation itself, of each profession. The fact of the existing social isolation in the collectives of specialized railroad cars was proved by conducting empirical studies in these collectives. The “Results” section presents a table that reflects the main psychogenic factors in the professions considered. Based on the data, a classification of labour social isolation was made. The classification of isolation types was represented by two groups: deprivation social isolation, rather acute isolation; irritable social isolation, less problematic but more common social isolation. The possibility of creating a universal “isolation passport” is discussed. All factors of social isolation of the group can be combined into three components: external factors; organizational and production factors; socio-psychological factors.
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Li, Xiao, Xinquan Wang, Hongguo Diao, He Zhang, and Chun Zhu. "Influence of Depth and Position of Vibration-Isolating Slot on Damping Effect of Blasting Vibration." Shock and Vibration 2023 (February 25, 2023): 1–11. http://dx.doi.org/10.1155/2023/8492360.
Повний текст джерелаАнотація:
The blasting vibration has an impact on the rock and soil around the blasting hole and the nearby structures. The vibration-isolating slot is one of the methods to reduce the damage of blasting vibration. In this paper, the influences of the depth and position of the vibration isolation slot on the PPV (peak particle velocity) attenuation characteristics of the ground vibration and blasting vibration damping effect of the vibration isolation slot at different measuring points are studied systematically by LS-DYNA numerical models. The results show that the damping effect of the vibration isolation slot is realized by reflecting the blasting vibration wave and increasing the propagation paths of the blasting vibration wave. When the measuring points are 5 m∼45 m far away from the vibration-isolating slot, the average vibration damping effect of the vibration-isolating slot is more than 30% and the deeper the depth of the vibration isolation slot, the better the vibration damping effect when the measuring points are 5 m∼15 m far away from the vibration isolation slot. However, when the measuring points are 15 m∼45 m far away from the vibration isolation slot, the vibration damping effect is best when the depth of the vibration isolation slot is 15 m. The vibration damping effect of blasting vibration at the same measuring point is different due to different positions of vibration isolation slot. For the measuring points M3 and M5, with the increasing of the distance between the vibration-isolating slot and the explosion source, the vibration damping effect of the vibration-isolating slot shows an increasing-decreasing-increasing trend; however, it shows a decreasing-increasing-decreasing trend for the measuring point M4.
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Suderman, Matthew, Mariko Moniwa, Tamiru N. Alkie, Davor Ojkic, Andre Broes, Neil Pople, and Yohannes Berhane. "Comparative Susceptibility of Madin–Darby Canine Kidney (MDCK) Derived Cell Lines for Isolation of Swine Origin Influenza A Viruses from Different Clinical Specimens." Viruses 13, no. 12 (November 23, 2021): 2346. http://dx.doi.org/10.3390/v13122346.
Повний текст джерелаАнотація:
Madin–Darby canine kidney (MDCK) cells are commonly used for the isolation of mammalian influenza A viruses. The goal of this study was to compare the sensitivity and suitability of the original MDCK cell line in comparison with MDCK-derived cell lines, MDCK.2, MDCK SIAT-1 and MDCK-London for isolation of swine-origin influenza A viruses (IAV-S) from clinical specimens. One-hundred thirty clinical specimens collected from pigs in the form of nasal swabs, lung tissue and oral fluids that were positive by PCR for the presence of IAV-S RNA were inoculated in the cell cultures listed above. MDCK-SIAT1 cells yielded the highest proportion of positive IAV-S isolations from all specimen types. For nasal swabs, 58.62% of the specimens were IAV-S positive in MDCK-SIAT1 cells, followed by MDCK-London (36.21%), and conventional MDCK and MDCK.2 cells (27.5%). For lung specimens, 59.38% were IAV-S positive in MDCK-SIAT1 cells, followed by MDCK-London (40.63%), and conventional MDCK and MDCK.2 cells (18.75–31.25%). Oral fluids yielded the lowest number of positive virus isolation results, but MDCK-SIAT1 cells were still had the highest rate (35%) of IAV-S isolation, whereas the isolation rate in other cells ranged from 5–7.5%. Samples with lower IAV-S PCR cycle threshold (Ct) values were more suitable for culturing and isolation. The isolated IAV-S represented H1N1-β, H1N2-α, H1N1pdm and H3N2 cluster IV and cluster IVB viruses. The result of the current study demonstrated the importance of using the most appropriate MDCK cells when isolating IAV-S from clinical samples.
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Reisige, T., T. Suckut, and S. W. Zuckut. "Rehabilitation of the Cathodic Protection System of a Sour Gas-Gathering System." Materials Performance 43, no. 8 (August 1, 2004): 24–27. https://doi.org/10.5006/mp2004_43_8-24.
Повний текст джерелаАнотація:
The South Oldenburg gas production district in northern Germany comprises reservoirs, operations sites, gathering and transfer stations, and underground pipelines. The district’s cathodic protection system underwent a major rehabilitation in the late 1990s. Electrical isolation was installed at separate facilities carrying different contents as well as pipelines having different coatings. Underground isolation was moved aboveground, and isolating flange kits replaced monolithic isolation joints. This article describes the rehabilitation process and the isolating fittings involved.
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R, Vetturayasudharsanan, Harini K K, Rajakumari S, Aarthy S, Sivashanmugam T, Vishalraj P V, Sarathkumar S, and Venkatesh A. "Analytical Study of Base Isolation- A Review." E3S Web of Conferences 399 (2023): 03018. http://dx.doi.org/10.1051/e3sconf/202339903018.
Повний текст джерелаАнотація:
Now a days the rate of happening of seismic events increasing and due to that so many structures got collapsed or damaged. In order to reduce the damage to structures during earthquakes, now a days the base isolation system is widely adopted and used over the world. This paper makes a wide review on the various base isolation techniques adopted and used. Different types of isolating bearings and materials used in it are reviewed. Here the review is done for the isolation system in normal R.C buildings (regular and irregular in plan) and also for bridges. The effect of base isolation system on some historic structures is also reviewed. The various advantages and disadvantages of different isolating bearings are reviewed. Here the effect of temperature on some isolating devices are also reviewed.
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Fu, Wei Qing, Fei Chen, and Hong Mei Wang. "Research on Numerical Simulation of Smart Base Isolation System with MR Damper(II)." Applied Mechanics and Materials 166-169 (May 2012): 2513–16. http://dx.doi.org/10.4028/www.scientific.net/amm.166-169.2513.
Повний текст джерелаАнотація:
Rubber bearing base-isolation is well-developed vibration reduction technique, and is applied in practical engineering broadly. But displacement of vibration isolating layer is large, so lead plug rubber bearing is used. It is passive control device; therefore its optimal central extent is narrow. That is isolating layer is optical in several defined kind and magnitude of earthquake waves, in the way controlled displacement and acceleration of superstructure is ideal. Magnetorheological damper has good smart performances- broad controlled band, fast response and demand a little energy. In this paper smart base isolation system, which is combined with rubber bearing and MR damper, is applied to structural vibration control, and have numerical simulation of structure employing LRB isolation and smart isolation in different kind and magnitude of earthquake waves. The result indicates smart base-isolation with MR damper can supply optimal control to these different earthquake waves, so is a good supplement and perfection for former isolation technique.
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Tanaka, N., and Y. Kikushima. "On the Hybrid Vibration Isolation Method." Journal of Vibration and Acoustics 111, no. 1 (January 1, 1989): 61–70. http://dx.doi.org/10.1115/1.3269824.
Повний текст джерелаАнотація:
For the purpose of isolating the vibration transmission from machines to soil and vice versa, this paper presents a new active vibration isolation method, that is, a hybrid vibration isolation method capable of isolating the vibration in the whole frequency range. The hybrid isolation method contains the following three characteristics: (1) a low-pass filter of a conventional elastic support method; (2) a high-pass filter of an active vibration isolation method; (3) suppression of a resonant peak due to an elastic support by a servo damper method; First, this paper presents the principle of the hybrid vibration isolation method and shows a fundamental structure of the system. Then by making the active isolator work as a damper, the suppression of a resonant peak due to an elastic support is achieved. Next, taking into consideration both the control effect and the stability of the system, the design procedure of the hybrid vibration isolation system is shown. Moreover, a control chart to estimate the control effect for suppressing the force transmissibility is presented. Finally, an experiment is carried out, demonstrating that the hybrid isolation method is capable of suppressing the exciting force in almost all the frequency range.
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Lamekhova, Elena Anatolyevna. "Methodology for isolation study as a factor of the evolutionary process for the university course «Theory of Evolution»." Samara Journal of Science 10, no. 4 (December 1, 2021): 273–79. http://dx.doi.org/10.17816/snv2021104306.
Повний текст джерелаАнотація:
The development of the synthetic theory of evolution, which arose on the basis of the synthesis of Darwinism, genetics and ecology, was accompanied by the discovery of evolutionary factors that were unknown in the 19thcentury. The discovery of isolation made it possible to describe the mechanism of conservation of populations, species, as well as the process of speciation. The educational literature on the theory of evolution provides definitions of isolation, a classification of isolation forms, and the role of isolation in the evolutionary process. The study of isolation as a factor in evolution requires a development of a methodology for isolating mechanisms study. The achievement of this goal is associated with the choice of a variant of isolation definition as a factor of evolution. Methodologically successful is the definition of isolation, which describes the role of this factor at the initial stages of microevolution and leads to the emergence of new species and the preservation of their isolation. When formulating the definition of isolation, it is advisable to use terms such as population, species, gene flow and divergence. The isolating mechanisms are manifested in several forms, which necessitate their classification. The classification of E.Mayr (1974) makes it possible to describe the sequence, relationship and role in the evolution of the main forms of isolation when conducting classes. From the point of view of teaching methods, laboratory work is recommended when considering such forms of isolation as seasonal, biotopic and ethological forms. The implementation of the proposed methodology for isolation study as a factor in the evolutionary process while doing the university course Theory of Evolution makes it possible to increase the effectiveness of the assimilation of educational material by students.
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Ramesh Babu Amathalai. "Relationship Between Workplace Isolation, Age and Turnover Intention amongst Malaysian Seafarers." Journal of Electrical Systems 20, no. 5s (April 13, 2024): 1614–25. http://dx.doi.org/10.52783/jes.2495.
Повний текст джерелаАнотація:
The study was carried out for the Malaysian Seafarers those having intention to quit their current career as a seafarer by discovering the relationships between the Workplace Isolation (WI) with the Turnover Intentions (TOI) concept and further to examine the moderation effect of age category over the correlation that showed significant relationships. Three sub workplace isolation elements are also tested (Social Isolation, Physical Isolation, and Informational Isolations) were assessed using the dimensional Impacts of Workplace Isolations. The P – E Fit Theory is then applied. All the measurements were collected from a sample of 400 registered Malaysian seafarers. There is a growing concern about shortage of Seafarers, particularly in Malaysia. This dissertation intends to clarify that being and feeling isolated is the main reason for these Seafarers to leave the seafaring career. This study also examines the seafarers age category as the moderating effects between the Workplace Isolation towards turnover intention among the seafarers in Malaysia. Moderation is divided in two distinct subgroups of ages. This study will help employers or the shipping companies in Malaysia to find solutions to this problem faced by the Seafarers by recommending certain measurers to counteract against turnover intention of the Seafarers.
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Bednarz, Jarosław. "The New Methodology for Assessing of the Applicability of Elastomeric Materials in the Vibration Isolation Systems of Railway Lines." Archives of Acoustics 41, no. 3 (September 1, 2016): 573–78. http://dx.doi.org/10.1515/aoa-2016-0055.
Повний текст джерелаАнотація:
Abstract The technical requirements for the determination of physical parameters of vibration isolating material have not been standardized in Europe and Poland yet, which significantly hinders the ability to compare vibration isolating materials offered on the market. Therefore, there is a need for establishing a norm that could be applied both for the determination of the physico-mechanical properties of elastic vibration isolation elements in rail transport for domestic and foreign producers as well as in their selection for application in a specific vibration isolation system. The paper presents a proposal to standardize the methodology of the estimation of vibration isolation materials physical parameters authorized for use in vibration isolation systems used in rail transport. Methodology for measuring the physico-mechanical parameters of vibration isolating material presented in the paper forms uniform test procedure developed based on a fragmentary norms for flexible materials testing. The use of the proposed research methodology enables the creation of a unified database of elastic materials which parameters will be easy to compare, and choice between them will become easier for designers of vibration isolation systems used in rail transport.
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Berkova, Zuzana, Frantisek Saudek, Peter Girman, Klara Zacharovova, Jan Kriz, Eva Fabryova, Ivan Leontovyc, et al. "Combining Donor Characteristics with Immunohistological Data Improves the Prediction of Islet Isolation Success." Journal of Diabetes Research 2016 (2016): 1–8. http://dx.doi.org/10.1155/2016/4214328.
Повний текст джерелаАнотація:
Variability of pancreatic donors may significantly impact the success of islet isolation. The aim of this study was to evaluate donor factors associated with isolation failure and to investigate whether immunohistology could contribute to organ selection. Donor characteristics were evaluated for both successful (n=61) and failed (n=98) islet isolations. Samples of donor pancreatic tissue (n=78) were taken for immunohistochemical examination. Islet isolations with 250000 islet equivalents were considered successful. We confirmed that BMI of less than 25 kg/m2(P<0.001), cold ischemia time more than 8 hours (P<0.01), hospitalization longer than 96 hours (P<0.05), higher catecholamine doses (P<0.05), and edematous pancreases (P<0.01) all unfavorably affected isolation outcome. Subsequent immunohistochemical examination of donor pancreases confirmed significant differences in insulin-positive areas (P<0.001). ROC analyses then established that the insulin-positive area in the pancreas could be used to predict the likely success of islet isolation (P<0.001). At the optimal cutoff point (>1.02%), sensitivity and specificity were 89% and 76%, respectively. To conclude, while the insulin-positive area, determined preislet isolation, as a single variable, is sufficient to predict isolation outcome and helps to improve the success of this procedure, its combination with the established donor scoring system might further improve organ selection.
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Hao, Jin Feng, Jian Hua Gao, Yang Liu, and Gui De Liu. "Spectrum Analysis of Isolated Vertical Seismic Response Storage Tanks." Applied Mechanics and Materials 166-169 (May 2012): 2270–74. http://dx.doi.org/10.4028/www.scientific.net/amm.166-169.2270.
Повний текст джерелаАнотація:
Aiming at the problem of seismic response of the base isolation tanks, make the tanks simplify the mechanical model of three particles, considering the quality of continuous liquid that is in the tanks equivalent to convection quality, pulse quality and rigid quality and introducing isolation stiffness. According to the stiffness of each particle and quality calculate natural vibration period, making sure the power amplification coefficient, and analysis on the response spectrum of isolation vertical seismic response storage tanks. The results show that the response spectrum method calculating isolation vertical seismic response storage tanks tends to safety；With the site categories and isolation cycle's increasing, basal shear shock absorption rate gradually reduce , the change of shaking height of wave is not obvious, after isolating the earthquake , hydraulic pressure show linear change, hydraulic pressure increase as the increasing of the site categories and ratio of height to radius, along with the increasing of the isolation of the cycle increasing, hydraulic pressure decrease；ratio of height to radius exists certain optimization period, in the period of optimization , basal shear shock absorption rate is bigger, isolating effect is better.
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Butaye, Patrick, Luc A. Devriese, Herman Goossens, Margaretha Ieven, and Freddy Haesebrouck. "Enterococci with Acquired Vancomycin Resistance in Pigs and Chickens of Different Age Groups." Antimicrobial Agents and Chemotherapy 43, no. 2 (February 1, 1999): 365–66. http://dx.doi.org/10.1128/aac.43.2.365.
Повний текст джерелаАнотація:
ABSTRACT Results for isolation of glycopeptide-resistant enterococci from fecal samples of pigs and chickens were found to differ strongly depending upon the type and age of animals and isolation technique (direct selective plate or broth enrichment). Isolations were frequent in broiler chickens and in sows but rare in layer chickens.
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Kozak, Genevieve M., Arthur B. Rudolph, Beatrice L. Colon, and Rebecca C. Fuller. "Postzygotic Isolation Evolves before Prezygotic Isolation between Fresh and Saltwater Populations of the Rainwater Killifish, Lucania parva." International Journal of Evolutionary Biology 2012 (January 30, 2012): 1–11. http://dx.doi.org/10.1155/2012/523967.
Повний текст джерелаАнотація:
Divergent natural selection has the potential to drive the evolution of reproductive isolation. The euryhaline killifish Lucania parva has stable populations in both fresh water and salt water. Lucania parva and its sister species, the freshwater L. goodei, are isolated by both prezygotic and postzygotic barriers. To further test whether adaptation to salinity has led to the evolution of these isolating barriers, we tested for incipient reproductive isolation within L. parva by crossing freshwater and saltwater populations. We found no evidence for prezygotic isolation, but reduced hybrid survival indicated that postzygotic isolation existed between L. parva populations. Therefore, postzygotic isolation evolved before prezygotic isolation in these ecologically divergent populations. Previous work on these species raised eggs with methylene blue, which acts as a fungicide. We found this fungicide distorts the pattern of postzygotic isolation by increasing fresh water survival in L. parva, masking species/population differences, and underestimating hybrid inviability.
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Cao, Jingdong, Shumin Zhang, Chengfu Ye, and Tianxia Jia. "Double-Star Isolation of Maximal Outerplanar Graphs ∗." Journal of Physics: Conference Series 2660, no. 1 (December 1, 2023): 012023. http://dx.doi.org/10.1088/1742-6596/2660/1/012023.
Повний текст джерелаАнотація:
Abstract In the graph G = (V, E), V represents the set of vertices and E represents the set of edges. ℱ represents a family of graphs. A subset S ⊆ V is considered an ℱ -isolating set if G[V\NG[S]] does not contain F as a subgraph for all F ∈ ℱ. The ℱ-isolation number of the graph G, denoted by 𝜄(G, ℱ), is defined as the minimum cardinality of an ℱ-isolating set in G. If the family ℱ consists of a single graph H, then the subset S is referred to as an H-isolating set. The H-isolation number of the graph G, denoted by 𝜄 H (G), is defined as the minimum cardinality of an H-isolating set in G. Maximal outerplanar graphs have been widely applied in different fields of research since 1891, where they hold significant importance. This paper aims to provide a comprehensive analysis of the H-isolation number in maximal outerplanar graphs, ι H ( G ) ≤ min { n 2 k + 4 , n + n 2 2 k + 5 , n − n 2 2 k + 2 } , where H ≅ S 1,k+1,k+1.
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Liu, Bo, Danguang Pan, Wanying Zhang, Yankai Lu, and Guowei Ni. "Mechanical Behaviors and Numerical Simulation Analysis of a New Isolation System." Applied Sciences 13, no. 13 (June 28, 2023): 7667. http://dx.doi.org/10.3390/app13137667.
Повний текст джерелаАнотація:
Finite element analysis is one of the key steps in evaluating the effectiveness of an isolation system. The aim of this paper is to research the mechanical properties of sliding and rolling friction systems and their application for isolating frame structures. A hybrid seismic isolation system is proposed, which aims to protect the superstructure by reducing dynamic response. The effect of different parameters on the friction coefficient of the isolation bearing was tested using a compression-shear testing machine, and the results showed that various parameters significantly affected the friction coefficient. In addition, a numerical analysis finite element model was established using SAP2000 software to compare the seismic isolation performance of the hybrid isolation system with that of traditional seismic-resistant structures. The results showed that the composite isolation system not only effectively reduced inter-story shear and acceleration, but also slightly enhanced the seismic isolation effect as the friction coefficient decreased.
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Zhang, Xin Zhong, Lian Juan Miao, and Ke Dong Tang. "Comparative Analysis of Seismic Responses of Different Layer Height Structures with Broken Stone Isolation Layers." Applied Mechanics and Materials 238 (November 2012): 876–80. http://dx.doi.org/10.4028/www.scientific.net/amm.238.876.
Повний текст джерелаАнотація:
For the purpose of doing modal and dynamic analysis, tall building structures and multistory building For the purpose of doing modal and dynamic analysis, tall building structures and multistory building structures of vibration isolation foundation and non-isolation models were established respectively. To elaborate the broken stone isolating layer efficacy, top ten order frequencies and modes, sliding displacements of isolation layer, storey drift angles, storey accelerations, top floor relative displacements compared to the ground movement are obtained and contrasted. The result of the present work implies that the broken stone isolation layers are suitable for multistory buildings.
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Sprague, Elliott, Steven Reynolds, and Peter Brindley. "Patient Isolation Precautions: Are They Worth It?" Canadian Respiratory Journal 2016 (2016): 1–5. http://dx.doi.org/10.1155/2016/5352625.
Повний текст джерелаАнотація:
Isolation precautions are intended to minimize pathogen transmission and reduce hospital-acquired infections. More recently, the effectiveness of isolation precautions has been questioned because of increasing evidence of risks. These putative downsides are divided into a quantifiable monetary cost (i.e., a literal cost to the system) and clinically important but less easily quantifiable costs (i.e., “costs” to the patient). The authors also briefly review deisolation and alternatives to isolation. The present review is not arguing against appropriate isolation or precautions, simply that the authors consider both risks and benefits and disseminate up-to-date information. Their patient-focused goal is to mitigate risks for those who truly need isolating and to end isolation as soon as it is safe and appropriate to do so.
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Lyon, James, Jocelyn E. Manning Fox, Aliya F. Spigelman, Ryekjang Kim, Nancy Smith, Doug O'Gorman, Tatsuya Kin, A. M. James Shapiro, Raymond V. Rajotte, and Patrick E. MacDonald. "Research-Focused Isolation of Human Islets From Donors With and Without Diabetes at the Alberta Diabetes Institute IsletCore." Endocrinology 157, no. 2 (December 11, 2015): 560–69. http://dx.doi.org/10.1210/en.2015-1562.
Повний текст джерелаАнотація:
Abstract Recent years have seen an increased focus on human islet biology, and exciting findings in the stem cell and genomic arenas highlight the need to define the key features of mature human islets and β-cells. Donor and organ procurement parameters impact human islet yield, although for research purposes islet yield may be secondary in importance to islet function. We examined the feasibility of a research-only human islet isolation, distribution, and biobanking program and whether key criteria such as cold ischemia time (CIT) and metabolic status may be relaxed and still allow successful research-focused isolations, including from donors with type 1 diabetes and type 2 diabetes. Through 142 isolations over approximately 5 years, we confirm that CIT and glycated hemoglobin each have a weak negative impacts on isolation purity and yield, and extending CIT beyond the typical clinical isolation cutoff of 12 hours (to ≥ 18 h) had only a modest impact on islet function. Age and glycated hemoglobin/type 2 diabetes status negatively impacted secretory function; however, these and other biological (sex, body mass index) and procurement/isolation variables (CIT, time in culture) appear to make only a small contribution to the heterogeneity of human islet function. This work demonstrates the feasibility of extending acceptable CIT for research-focused human islet isolation and highlights the biological variation in function of human islets from donors with and without diabetes.
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Postiglioni, Rodrigo, and Fernando G. Costa. "Reproductive isolation among three populations of the genus Grammostola from Uruguay (Araneae, Theraphosidae)." Iheringia. Série Zoologia 96, no. 1 (March 2006): 71–74. http://dx.doi.org/10.1590/s0073-47212006000100013.
Повний текст джерелаАнотація:
Ethological isolation of individuals from three allopatric Grammostola populations of Uruguay, G. iheringi (Keyserling, 1891), G. mollicoma (Auserer, 1875) northern population and G. mollicoma southern population, was tested under laboratory conditions. Grammostola iheringi behaved as a reproductive isolated species, whereas the two populations of G. mollicoma did not show ethological isolation between them. However, ecological isolating reproductive mechanisms could be acting on G. mollicoma populations. Artificial burrows seem to be important for reproductive isolation in these species.
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Gompert, Zachariah, Thomas L. Parchman, and C. Alex Buerkle. "Genomics of isolation in hybrids." Philosophical Transactions of the Royal Society B: Biological Sciences 367, no. 1587 (February 5, 2012): 439–50. http://dx.doi.org/10.1098/rstb.2011.0196.
Повний текст джерелаАнотація:
Hybrid zones are common in nature and can offer critical insights into the dynamics and components of reproductive isolation. Hybrids between diverged lineages are particularly informative about the genetic architecture of reproductive isolation, because introgression in an admixed population is a direct measure of isolation. In this paper, we combine simulations and a new statistical model to determine the extent to which different genetic architectures of isolation leave different signatures on genome-level patterns of introgression. We found that reproductive isolation caused by one or several loci of large effect caused greater heterogeneity in patterns of introgression than architectures involving many loci with small fitness effects, particularly when isolating factors were closely linked. The same conditions that led to heterogeneous introgression often resulted in a reasonable correspondence between outlier loci and the genetic loci that contributed to isolation. However, demographic conditions affected both of these results, highlighting potential limitations to the study of the speciation genomics. Further progress in understanding the genomics of speciation will require large-scale empirical studies of introgression in hybrid zones and model-based analyses, as well as more comprehensive modelling of the expected levels of isolation with different demographies and genetic architectures of isolation.
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Zhang, Haiyang, Qi Zhang, Yuanyuan Deng, Mengxi Chen, and Chenxi Yang. "Improving Isolation of Extracellular Vesicles by Utilizing Nanomaterials." Membranes 12, no. 1 (December 31, 2021): 55. http://dx.doi.org/10.3390/membranes12010055.
Повний текст джерелаАнотація:
Extracellular vesicles (EVs) as the new form of cellular communication have been demonstrated their potential use for disease diagnosis, prognosis and treatment. EVs are vesicles with a lipid bilayer and are present in various biofluids, such as blood, saliva and urine. Therefore, EVs have emerged as one of the most appealing sources for the discovery of clinical biomarkers. However, isolation of the target EVs from different biofluids is required for the use of EVs as diagnostic and therapeutic entities in clinical settings. Owing to their unique properties and versatile functionalities, nanomaterials have been widely investigated for EV isolation with the aim to provide rapid, simple, and efficient EV enrichment. Herein, this review presents the progress of nanomaterial-based isolations for EVs over the past five years (from 2017 to 2021) and discusses the use of nanomaterials for EV isolations based on the underlying mechanism in order to offer insights into the design of nanomaterials for EV isolations.
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Zheng, Pei. "Container Crane Seismic Isolation Device Design Method Based on Structural Dynamics." Applied Mechanics and Materials 353-356 (August 2013): 1819–25. http://dx.doi.org/10.4028/www.scientific.net/amm.353-356.1819.
Повний текст джерелаАнотація:
Based on container cranes seismic load calculation criteria with structural dynamics and damage modes of container crane under earthquake, the seismic isolation device of this over-span flexible structure and its associated parameters selection were investigated. Taking the first crane equipped with isolating device as an example, through dynamic analysis and simulating to crane flexible system with isolation device under the earthquake, efforts was made to research for suitable isolation device and design method for port crane. The calculation and analysis in the present study could provide a new way for the container cranes isolation device design and assessment of the structural anti-seismic effect
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Lowry, David B., Jennifer L. Modliszewski, Kevin M. Wright, Carrie A. Wu, and John H. Willis. "The strength and genetic basis of reproductive isolating barriers in flowering plants." Philosophical Transactions of the Royal Society B: Biological Sciences 363, no. 1506 (June 25, 2008): 3009–21. http://dx.doi.org/10.1098/rstb.2008.0064.
Повний текст джерелаАнотація:
Speciation is characterized by the evolution of reproductive isolation between two groups of organisms. Understanding the process of speciation requires the quantification of barriers to reproductive isolation, dissection of the genetic mechanisms that contribute to those barriers and determination of the forces driving the evolution of those barriers. Through a comprehensive analysis involving 19 pairs of plant taxa, we assessed the strength and patterns of asymmetry of multiple prezygotic and postzygotic reproductive isolating barriers. We then reviewed contemporary knowledge of the genetic architecture of reproductive isolation and the relative role of chromosomal and genic factors in intrinsic postzygotic isolation. On average, we found that prezygotic isolation is approximately twice as strong as postzygotic isolation, and that postmating barriers are approximately three times more asymmetrical in their action than premating barriers. Barriers involve a variable number of loci, and chromosomal rearrangements may have a limited direct role in reproductive isolation in plants. Future research should aim to understand the relationship between particular genetic loci and the magnitude of their effect on reproductive isolation in nature, the geographical scale at which plant speciation occurs, and the role of different evolutionary forces in the speciation process.
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Tsuchiya, Takahiro, Takeshi Akasaka, and Shigeru Nagase. "New vistas in fullerene endohedrals: Functionalization with compounds from main group elements." Pure and Applied Chemistry 82, no. 3 (February 19, 2010): 505–21. http://dx.doi.org/10.1351/pac-con-09-08-14.
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This paper describes the convenient method of isolating endohedral metallofullerenes by means of selective reduction from carbon soot extracts. Successful isolation in large amount by utilizing this method allows examination of the selective chemical modification of monometallofullerenes. Furthermore, isolation of missing metallofullerenes as their derivatives is also mentioned.
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Omarov, Khadjimurad M., and Abakar D. Abakarov. "The Seismic Response Investigation on the Multi-Storey Buildings with Seismically Insulating Rubber-Metal Supports." Materials Science Forum 931 (September 2018): 362–67. http://dx.doi.org/10.4028/www.scientific.net/msf.931.362.
Повний текст джерелаАнотація:
The buildings of various constructive solutions with seismic isolation and without seismic isolation are considered. The system of differential equations of a multi-storey building motion with seismic isolating rubber-metal supports is presented. The graphs of the seismic response of 5, 9 and 12-storey buildings with seismic insulating rubber-metal bearings are obtained as well as with seismic action in the form of the unsteady random process. The effectiveness of seismic isolation systems with rubber-metal bearings with lead core and without it is estimated.
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Grimes, James E. "Enigmatic psittacine chlamydiosis: Results of serotesting and isolation attempts, 1978 through 1983, and considerations for the future." Journal of the American Veterinary Medical Association 186, no. 10 (May 15, 1985): 1075–79. https://doi.org/10.2460/javma.1985.186.10.1075.
Повний текст джерелаАнотація:
SUMMARY From 1978 through 1983, chlamydiosis was diagnosed by isolation of Chlamydia psittaci from various types of psittacine birds. The organism was isolated from 126 (30.4%) of 414 tissue specimens, with the percentages ranging from 12.5% (budgerigars) to 42.8% (cockatiels), excluding 2 parakeets with 1 isolation (50%). From 1,035 cloacal swab/feces specimens, 51 (4.9%) isolations were made, ranging from 1.4% from African grays (1 of 70) to 27.8% from lovebirds (5 of 18). Positive direct microscopic examination of stained (Gimenez method) tissue impressions correlated with positive isolation at a rate of 79.2% and those found negative by direct examination had a correlation of 87.5%. Direct complement-fixation testing was done on 3,485 sera. Forty-six were unsatisfactory for testing due to their being anticomplementary or reacting with control antigen. The distribution of titers ranged from 2,008 (57.6%) at 8 to 76 (2.2%) at ≥256. In serotests and isolation attempts from the same bird, there was 42.8% agreement between titers ≥32 and positive isolation. One cockatiel with a complement-fixation titer of 16 yielded a positive isolation, whereas other types of birds with a ≤ 16 titer were negative.
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Stephan Hellmig, Stephan Hellmig, Kai Schindewolf, and Andre Bode. "Role of multiplex PCR in the management of acute infectious diarrhea in a hospital setting – a single center retrospective study." Gastroenterology & Hepatology: Open Access 14, no. 2 (March 15, 2023): 37–38. http://dx.doi.org/10.15406/ghoa.2023.14.00542.
Повний текст джерелаАнотація:
Background and Aim Isolation measures in patients with acute diarrhea produce enormous costs of materials and personnel. Aim of this retrospective study was to evalute the percentage of unnecessary isolations and the impact of multiplex PCR stool testing on isolation days before and after introduction into clinical practice. Methods: Between July 2020 and July 2022 in total 2373 stool specimens of patients with acute diarrhea and a course no longer than 14 days were analyzed using BioFire® FilmArray® Gastrointestinal (GI) Panel. Number of isolation beds per day were compared before and after the introduction of Multiplex PCR testing. Results: In 65% of all specimen examined no pathogen was detected. Single-room isolation was a necessary in only 22% of all cases. Isolation days on the gastroenterological ward decreased from 635 in 2019 to 384 in 2020, a reduction of 39,5%. Conclusion: In acute infectious diarrhea Multiplex PCR is a use full tool to rapidly identify the causative agent and exclude the necessity of isolation in the majority of cases. In spite of all medical and economical advantages Multiplex PCR testing has not been established widely so far.
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Nasare, Ruchita, Sakshi Garad, Sakshi Patil, and Nikita Besekar. "Fault Detection and Isolation ofDCMicro-Grid." April-May 2023, no. 33 (May 25, 2023): 31–39. http://dx.doi.org/10.55529/jeet.33.31.39.
Повний текст джерелаАнотація:
In a microgrid, faults can lead to voltage instability, which can produce voltage sags, swells, or even a full collapse of the voltage. This may result in electrical equipment damage and interfere with the microgrid's operation. Faults can also produce safety risks including fire, explosion, or electrocution, which can endanger employees and harm equipment. By isolating or shutting down the system when a malfunction is found, the danger of harm or injury is reduced. The purpose of fault isolation and detection in a DC microgrid is to guarantee the microgrid's dependability and safety. The microgrid's flaws are found using fault detection and isolation (FDI) techniques, which are then used to isolate them so they won't influence other system components. Themicrogrid's downtime can be reduced by immediately identifying and isolating issues, ensuring a steady supply of electricity to the associated loads. Additionally, fault isolation and detection can stop problems from spreading to other system components, which can reduce the severity of the fault's harm.The purpose of this research is to identify and isolate faults that occur in DC microgrids, as well as to minimise their impact. In this project fault detection and isolation is properly done, for detecting a fault microprocessor is used which continuously monitor the values of current and voltage and for isolation of microgrid relay is used. We are successfully providing the circuit which detects the fault and isolate within the time which reduces the severity of fault and protect the healthy
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Li, Xiao Quan. "A Fabrication of the High Emitter-Isolating-Layer for Printed Cold Cathode in Field Emission Display Panel." Advanced Materials Research 459 (January 2012): 567–70. http://dx.doi.org/10.4028/www.scientific.net/amr.459.567.
Повний текст джерелаАнотація:
Using black insulation slurry as manufacture material, the high emitter-isolating-layer was fabricated on the cathode plate. The high emitter-isolating-layer which possessed good insulation performance was composed of many parallel emitter-isolation-stripes, which the bottom cathode electrode would be exposed in the center of the emitter-isolation-stripe. The carbon nanotube paste was prepared onto the cathode plate to form the field emitters. And the field emission display panel with high emitter-isolating-layer was demonstrated, which the better field emission property including the large emission current and stable electron emission was confirmed.
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Baechler, Raffaela. "Complexity, Isolation, and Language Change." Zeitschrift für Dialektologie und Linguistik 84, no. 2-3 (2017): 178–201. http://dx.doi.org/10.25162/zdl-2017-0009.
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Hamler, Tyrone C., Ann W. Nguyen, Harry Owen Taylor, Weidi Qin, and Fei Wang. "Gender Differences in Objective and Subjective Social Isolation and Self-Reported Hypertension in Older Adults." Journal of Cardiovascular Development and Disease 12, no. 4 (April 4, 2025): 136. https://doi.org/10.3390/jcdd12040136.
Повний текст джерелаАнотація:
Hypertension is a major public health concern, especially in older adults, and gender differences are a factor in terms of its prevalence. Social connections benefit health, while social isolation is linked to negative outcomes. Prior studies suggest social isolation and connectedness vary by gender, but few have explored this relationship with hypertension. This study examined gender differences in the association between social isolation and hypertension in older adults using data from the National Survey of American Life (1280 adults aged ≥55). Weighted logistic regressions tested gender differences in objective and subjective social isolation and hypertension. Both men and women who were objectively isolated from family and friends, or only friends, were less likely to have hypertension than those not isolated. However, when accounting for subjective isolation, only isolation from family predicted hypertension. Gender moderated this relationship—men isolated from family and friends had a higher likelihood of hypertension, while no such association was found for women. Findings suggest that preventing objective isolation, particularly from family, may help reduce hypertension risk in older adults. This study highlights the need to further investigate social isolation’s impact on health and its underlying mechanisms among older adults in the U.S.
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Mojtabai, A. G. "Isolation." Antioch Review 55, no. 4 (1997): 474. http://dx.doi.org/10.2307/4613581.
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Ann Phong. "Isolation." Frontiers: A Journal of Women Studies 37, no. 2 (2016): 213. http://dx.doi.org/10.5250/fronjwomestud.37.2.0213.
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McNally, Steve. "Isolation." Learning Disability Practice 15, no. 7 (August 30, 2012): 11. http://dx.doi.org/10.7748/ldp.15.7.11.s8.
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Edmond, Michael. "Isolation." Infection Control and Hospital Epidemiology 18, no. 1 (January 1997): 58–64. http://dx.doi.org/10.2307/30141965.
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O'Shea, Saoirse Caitlin. "Isolation." Gender, Work & Organization 27, no. 5 (June 10, 2020): 717–22. http://dx.doi.org/10.1111/gwao.12464.
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