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Статті в журналах з теми "Isozyme polymorphism"

Автор: Grafiati

Опубліковано: 3 червня 2025

Оновлено: 31 липня 2025

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1

ANIEL KUMAR, Owk, Sape S. TATA, and Kancharla PAVAN KUMAR. "Analysis of Several Popular Cultivars of Madagascar Periwinkle (Catharanthus roseus (L.) G. Don.) using Biochemical Markers." Notulae Scientia Biologicae 5, no. 4 (2013): 458–61. http://dx.doi.org/10.15835/nsb549178.

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Анотація:

Band designs of esterase (EST), peroxidase (PO) and polyphenol oxidase (PPO) isozymes in several selected cultivars of Catharanthus roseus by using native polyacrylamide gel electrophoresis (PAGE) were investigated in this study. It was confirmed that cultivar differences in isozyme polymorphism can be revealed by applied electrophoretic patterns. Three isozyme systems produced a total of 16 bands with polymorphism ranged from 66.6-100%. Considering the patterns of isozyme variations in the five cultivars of Catharanthus roseus, it is evident that the cultivar ‘First kiss coral’ displayed crim

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2

WEEDEN, N. F., and A. C. EMMO. "ISOZYME CHARACTERIZATION OF KENTUCKY BLUEGRASS CULTIVARS." Canadian Journal of Plant Science 65, no. 4 (1985): 985–94. http://dx.doi.org/10.4141/cjps85-126.

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Twenty-two cultivars of Kentucky bluegrass (Poa pratensis L.) were examined for their glucose phosphate isomerase, triose phosphate isomerase, phosphoglucomutase, malate dehydrogenase, 6-phosphogluconate dehydrogenase and alpha-naphthyl esterase phenotypes. Polymorphism was observed in each isozyme system, and the first four systems were characterized for variation among and within the cultivars. Sixteen of the 22 cultivars could be uniquely identified by selecting isozymes which displayed clearly resolved bands and exhibited little or no intracultivar polymorphism. Analysis could be performed

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3

Degani, Chemda, Ruth El-Batsri, and Shmuel Gazit. "Enzyme Polymorphism in Mango." Journal of the American Society for Horticultural Science 115, no. 5 (1990): 844–47. http://dx.doi.org/10.21273/jashs.115.5.844.

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Forty-one (Mangifera indica L.) cultivars were characterized electrophoretically using the isozyme systems aconitase, isocitrate dehydrogenase, leucine aminopeptidase, phosphoglucose isomerase, phosphoglucomutase, and triosephosphate isomerase. The outcross origin of some of the mango cultivars was supported by the isozymic banding patterns. Reported parentage of some other cultivars was not consistent with their isozymic banding patterns.

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4

Samimy, Cyrus, and James N. Cummins. "Distinguishing Apple Rootstocks by Isozyme Banding Patterns." HortScience 27, no. 7 (1992): 829–31. http://dx.doi.org/10.21273/hortsci.27.7.829.

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Isozymes of six enzyme systems extracted from 13 apple (Malus domestica Borkh.) rootstocks were separated electrophoretically on a horizontal starch gel. Each rootstock was clearly distinguished by its unique isozyme banding patterns. All the rootstocks were distinguishable using only two of the enzyme systems, phosphoglucomutase and 6-phosphogluconate dehydrogenase, both of which exhibited considerable isozyme polymorphism.

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5

Haque, ME, MA Islam, and B. Sikdar. "Molecular divergence of locally grown pumpkin (Cucurbita maxima) cultivars through some isozyme tests." Journal of Bio-Science 19 (December 19, 2012): 89–93. http://dx.doi.org/10.3329/jbs.v19i0.13006.

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Context: Pumpkin (Cucurbita maxima) is highly polymorphic vegetable species and its polymorphism can be analyzed by isozyme molecular marker. Objective: To analyze genetic polymorphism among 10 locally grown pumpkin cultivars by isozyme. Materials and Methods: Fresh leaves of young plant of different cultivars were used for enzyme extraction. Enzyme extracts were prepared by homogenizing of 2 g sample of each cultivar. Prechilled mortar and pestle nestled in ice along with 1% polyvinylpyrrolidone and 2 ml of chilled extraction buffer were used prior to centrifuge. N-PAGE was conducted for diff

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6

Degani, C., A. Beiles, R. El-Batsri, M. Goren, and S. Gazit. "Identifying Lychee Cultivars by Isozyme Analysis." Journal of the American Society for Horticultural Science 120, no. 2 (1995): 307–12. http://dx.doi.org/10.21273/jashs.120.2.307.

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Leaf isozyme banding patterns were studied in 30 cultivars and selections of lychee (Litchi Chinensis Sonn.) by means of starch gel electrophoresis. Polymorphism in aconitase, aspartate aminotransferase, isocitrate dehydrogenase, phosphoglucomutase, shikimate dehydrogenase, superoxide dismutase and triosephosphate isomerase is demonstrated for the first time and observations are extended for the previously described polymorphism in phosphoglucose isomerase. In this study we found five groups of cultivars with identical electrophoretic genotypes. The 18 different cultivars were clustered by the

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7

Ipek, Meryem, Ahmet Ipek, and Philipp W. Simon. "Comparison of AFLPs, RAPD Markers, and Isozymes for Diversity Assessment of Garlic and Detection of Putative Duplicates in Germplasm Collections." Journal of the American Society for Horticultural Science 128, no. 2 (2003): 246–52. http://dx.doi.org/10.21273/jashs.128.2.0246.

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Garlic (Allium sativum L.) is an asexually propagated crop that displays much morphological diversity. Studies which have assessed garlic diversity with isozymes and randomly amplified polymorphic DNA (RAPD) markers generally agreed with the morphological observations but sometimes failed to discriminate clones. To discriminate among closely related garlic clones in more detail, we introduced amplified fragment-length polymorphism (AFLPs) to evaluate the genetic diversity and phenetic relatedness of 45 garlic clones and three A. longicuspis clones and we compared AFLP results with RAPD markers

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8

Ipek, Meryem, Ahmet Ipek, and Philipp W. Simon. "Comparison of AFLPs, RAPD Markers, and Isozymes for Diversity Assessment of Garlic and Detection of Putative Duplicates in Germplasm Collections." Journal of the American Society for Horticultural Science 128, no. 2 (2003): 246–52. https://doi.org/10.21273/jashs.128.2.246.

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Анотація:

Garlic (Allium sativum L.) is an asexually propagated crop that displays much morphological diversity. Studies which have assessed garlic diversity with isozymes and randomly amplified polymorphic DNA (RAPD) markers generally agreed with the morphological observations but sometimes failed to discriminate clones. To discriminate among closely related garlic clones in more detail, we introduced amplified fragment-length polymorphism (AFLPs) to evaluate the genetic diversity and phenetic relatedness of 45 garlic clones and three A. longicuspis clones and we compared AFLP results with RAPD markers

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9

Weeden, Norman F., Bruce I. Reisch, and Mary-Howell E. Martens. "Genetic Analysis of Isozyme Polymorphism in Grape." Journal of the American Society for Horticultural Science 113, no. 5 (1988): 765–69. http://dx.doi.org/10.21273/jashs.113.5.765.

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Abstract Genetic analysis of 11 allozyme polymorphisms was performed on the progeny of ‘Cayuga White’ × ‘Aurora’, two complex interspecific grape (Vitis) hybrids. Segregation for most of the polymorphisms closely approximated monogenic Mendelian ratios, and eight new isozyme loci were defined for grape. Joint segregation analysis among the isozyme loci revealed three multilocus linkage groups. These results demonstrate that sufficient allozyme polymorphism exists in grape to establish many multilocus linkage groups and that this genetic analysis can be accomplished using extant progeny or prog

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10

Sun, Gen-Lou, Oscar Díaz, Björn Salomon, and Roland von Bothmer. "Genetic diversity in Elymus caninus as revealed by isozyme, RAPD, and microsatellite markers." Genome 42, no. 3 (1999): 420–31. http://dx.doi.org/10.1139/g98-130.

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Genetic diversity of 33 Elymus caninus accessions was investigated using isozyme, RAPD, and microsatellite markers. The three assays differed in the amount of polymorphism detected. Microsatellites detected the highest polymorphism. Six microsatellite primer pairs generated a total of 74 polymorphic bands (alleles), with an average of 15.7 bands per primer pair. Three genetic similarity matrices were estimated based on band presence or absence. Genetic diversity trees (dendrograms) were derived from each marker technique, and compared using Mantel's test. The correlation coefficients were 0.20

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11

Weeden, N. F., and R. C. Lamb. "Identification of Apple Cultivars by Isozyme Phenotypes." Journal of the American Society for Horticultural Science 110, no. 4 (1985): 509–15. http://dx.doi.org/10.21273/jashs.110.4.509.

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Abstract Fifty-four apple (Malus domestica Borkh.) cultivars were characterized electrophoretically using 6 isozyme systems. Intracultivar variation in isozyme phenotype was not observed, whereas intercultivar polymorphism was sufficient to permit reliable and unambiguous identification of nearly every cultivar. The most useful isozyme systems for distinguishing among the cultivars were 6-phosphogluconate dehydrogenase and aspartate aminotransferase. Sports could not be distinguished from the original cultivar. The genetic basic of several polymorphisms was known, enabling the comparison of th

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12

O'Leary, Maureen C., and Thomas H. Boyle. "Isozyme Polymorphism and Inheritance in Rhipsalidopsis and Schlumbergera (Cactaceae)." HortScience 30, no. 4 (1995): 856G—857. http://dx.doi.org/10.21273/hortsci.30.4.856g.

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Анотація:

Cultivars and seedlings of Rhipsalidopsis and Schlumbergera were subjected to isozyme analysis using seven enzyme systems [aspartate aminotransferase (AAT), aminopeptidase (AMP), glucose-6-phosphate isomerase (GPI), malate dehydrogenase (MDH), phosphoglucomutase (PGM), shikimate dehydrogenase (SKD), and triose phosphate isomerase (TPI)]. Isozymes were extracted from phylloclades and roots, and were separated by polyacrylamide gel electrophoresis (PAGE) using single percentage (5% to 10%) gels. Six enzymes exhibited polymorphism in Rhipsalidopsis, whereas all seven enzymes were polymorphic in S

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13

O'Leary, Maureen C., and Thomas H. Boyle. "Isozyme Polymorphism and Inheritance in Rhipsalidopsis and Schlumbergera (Cactaceae)." HortScience 30, no. 4 (1995): 856G—857. http://dx.doi.org/10.21273/hortsci.30.4.856.

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Анотація:

Cultivars and seedlings of Rhipsalidopsis and Schlumbergera were subjected to isozyme analysis using seven enzyme systems [aspartate aminotransferase (AAT), aminopeptidase (AMP), glucose-6-phosphate isomerase (GPI), malate dehydrogenase (MDH), phosphoglucomutase (PGM), shikimate dehydrogenase (SKD), and triose phosphate isomerase (TPI)]. Isozymes were extracted from phylloclades and roots, and were separated by polyacrylamide gel electrophoresis (PAGE) using single percentage (5% to 10%) gels. Six enzymes exhibited polymorphism in Rhipsalidopsis, whereas all seven enzymes were polymorphic in S

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14

Bosland, Paul W., and Paul H. Williams. "An evaluation of Fusarium oxysporum from crucifers based on pathogenicity, isozyme polymorphism, vegetative compatibility, and geographic origin." Canadian Journal of Botany 65, no. 10 (1987): 2067–73. http://dx.doi.org/10.1139/b87-282.

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A global collection of 123 putative isolates of Fusarium oxysporum from crucifers was examined for pathogenicity, isozyme polymorphism, and vegetative compatibility. Of these isolates, 103 were found to be pathogenic on one or more of six differential crucifer cultivars. Three patterns of isozyme polymorphism (electrophoretic types) were found and by means of a nitrate reductase complementation test, three major vegetative compatibility groups were identified that could differentiate among the F. oxysporum pathotypes. Complete correspondence was found among pathotype, electrophoretic type, and

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15

Syah, Titis Hutama, and Arbain Arbain. "PENGEMBANGAN METODE PENANDA ISOZYME PADA TREMBESI." Jurnal Pemuliaan Tanaman Hutan 14, no. 2 (2020): 137–45. http://dx.doi.org/10.20886/jpth.2020.14.2.137-145.

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Fifty samples of raintree from Sangatta, the capital city of Kutai Timur Regency, East Kalimantan were analyzed using isozyme markers to determine the characteristics of the banding pattern. The use of isozymes was intended as a biochemical marker for genetic diversity analysis. This study aimed to determine the enzyme system that could be used to determine genetic diversity of raintree. The enzyme systems used were diaphorase, esterase, and peroxidase. Polymorphism assessments were carried out on the parameters of expected heterozygosity (H), polymorphism information content (PIC), effective

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16

Resende, Adriana Gazoli, Pedro Soares Vidigal Filho, and Maria de Fátima P. S. Machado. "Esterase polymorphism marking cultivars of Manihot esculenta, Crantz." Brazilian Archives of Biology and Technology 47, no. 3 (2004): 347–53. http://dx.doi.org/10.1590/s1516-89132004000300003.

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Esterase isozymes were used to detected substrate-preference polymorphism in twenty cultivars of Manihot esculenta, and to show cultivar-specific variation of this species. A relatively complex extraction solution of proteins from leaves was needed to show a larger number of esterase isozymes. Similarity between cultivars from six groups ranged from 51 to 96%. The cultivars identified by the same name seemed to be biochemically different regarding esterase isozymes. Esterase isozyme electrophoretic patterns could, therefore, be used to discriminate the cultivars identified by the same name, an

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17

Sun, W., and J. L. Brewbaker. "ISOZYME POLYMORPHISM IN LEUCAENA SPECIES." HortScience 27, no. 6 (1992): 646c—646. http://dx.doi.org/10.21273/hortsci.27.6.646c.

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The Leguminous Leucaena including 15 species, of which several have become pantropical as fodder, fuelwood, shade and ornamental trees. 160 collections of 12 Leucaena species, including 3 tetraploids and 9 diploids, were analyzed for six isozyme systems. Extensive inter and intraspecific variability was detected using cotyledon tissues. The uniformity of isozymic expression in 80 collections of the “common leucaena” from all over the world supported the hypothesis that it is a single self-pollinated variety. It was first distributed to the Philippines from Mexico in late 1500s, then spread wor

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18

Panetta, FD. "Isozyme Variation in Australian and South-African Populations of Emex australis Steinh." Australian Journal of Botany 38, no. 2 (1990): 161. http://dx.doi.org/10.1071/bt9900161.

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Isozyme variation was surveyed at 25 loci in 65 Australian (colonial) and 21 South African (native) populations of Emex australis. Only one polymorphism, restricted in distribution to the eastern States, was observed in Australia. Three additional polymorphisms were detected in South African populations, but most (16) South African populations were indistinguishable from the Australian ones. Thus, the relative uniformity of colonial populations of E. australis reflects the low level of isozyme variation in many populations within its native range.

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19

McCoy, T. J., C. S. Echt, and L. C. Mancino. "Segregation of molecular markers supports an allotetraploid structure for Medicago sativa × Medicago papillosa interspecific hybrid." Genome 34, no. 4 (1991): 574–78. http://dx.doi.org/10.1139/g91-088.

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Cytogenetic analysis has indicated there is little genomic affinity between the genomes of Medicago sativa L. and Medicago papillosa Boiss. The objective of this study was to determine whether disomic segregation of alleles at isozyme and restriction fragment length polymorphism (RFLP) loci occurs in F1 hybrids of M. sativa × M. papillosa. We examined segregation of alleles at seven isozyme loci and 13 RFLP loci. Of the 20 loci analyzed, 11 exhibited a disomic pattern of inheritance, indicative of strict species-specific chromosome pairing in the M. sativa × M. papillosa hybrids. The other nin

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20

Carvalho, Jane Fiuza Rodrigues Portela de, Ingrid Peters Robinson, and Acelino Couto Alfenas. "Isozymic variability in a Brazilian collection of annatto (Bixa orellana L.)." Pesquisa Agropecuária Brasileira 40, no. 7 (2005): 653–60. http://dx.doi.org/10.1590/s0100-204x2005000700005.

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The objectives of this work were to optimize the isozyme electrophoresis technique for Bixa orellana, and use isozyme markers for a preliminary survey on the genetic variability in Brazilian annatto germplasm accessions. Collection consisted of seed samples from sixty open pollinated trees, representing two Northern and four Southern geographic provenances. The extraction, electrophoresis, and interpretation of annatto isozymes are described. Three out of the twenty-one identified isozyme loci were polymorphic in the collection. The percentage of polymorphic loci (P = 21.05) and the expected h

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21

Hoffman, David L., Douglas E. Soltis, F. J. Muehlbauer, and G. Ladizinsky. "Isozyme Polymorphism in Lens (Leguminosae)." Systematic Botany 11, no. 3 (1986): 392. http://dx.doi.org/10.2307/2419075.

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22

Battistini, S., and S. Sansavini. "ISOZYME POLYMORPHISM IN APRICOT CULTIVARS." Acta Horticulturae, no. 293 (September 1991): 259–66. http://dx.doi.org/10.17660/actahortic.1991.293.30.

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23

Livesey, V., and J. Norrington-Davies. "Isozyme polymorphism in Festuca rubraL." Euphytica 55, no. 1 (1991): 73–79. http://dx.doi.org/10.1007/bf00022562.

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24

Sinaga, Soaloon, Sobir Sobir, Roedhy Poerwanto, Hajrial Aswidinnoor, and Dedy Duryadi. "Genetic Diversity and The Relationship Between The Indonesian Mangosteen (Garcinia Mangostana) and The Related Species Using Isozyme Markers." Jurnal Natur Indonesia 13, no. 1 (2012): 53. http://dx.doi.org/10.31258/jnat.13.1.53-58.

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Indonesia was known to have high diversity of mangosteens (Garcinia mangostana) and the related species. Inorder to elucidate the genetics variability of the diversity, thirty three accessions were examined by using isozymeanalysis. The genetic diversity and relationships among several mangosteens and other Garcinia sp wereestablished by using four isozymes. The level of polymorphism as revealed by isoenzyme was 88%. Althoughmangosteen is believed to reproduce exclusively through apomixis, our result show that considerable geneticdiversity exists within G. mangostana and between other Garcinia

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25

Darmo, E., and M. S. Strefeler. "CHARACTERIZATION OF ISOZYME DIVERSITY WITHIN AND AMONG PURPLE LOOSESTRIFE POPULATIONS (LYTHRUM SPP.)." HortScience 27, no. 6 (1992): 586a—586. http://dx.doi.org/10.21273/hortsci.27.6.586a.

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Purple loosestrife populations have developed into a highly aggressive and invasive weed in several Northern states (MN, NY, NJ etc.). How these populations arose is a key question in developing control strategies. Therefore, we initiated a study to elucidate the origin and genetic structure of invasive populations using isozyme analysis. The germplasm examined included invasive populations found in MN, NY, NJ, WI and MD, populations of Lythrum alatum, populations of L. virgatum and 22 cultivars of L. salicaria, the suspected progenitor of the invasive populations. Unique isozyme patterns for

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26

Lebeda, A., I. Doležalová, M. Dziechciarková, K. Doležal, and J. Frček. "Morphological Variability and Isozyme Polymorphisms in Maca and Yacon." Czech Journal of Genetics and Plant Breeding 39, No. 1 (2011): 1–8. http://dx.doi.org/10.17221/3714-cjgpb.

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A set of 15 accessions of maca, Lepidium meyenii Walp., and 25 accessions of yacon, Smallanthus sonchifolius (Poepp. &amp; Endl.) H. Robins., cultivated under Czech field conditions was studied to determine relationships between morphological variability and isozyme polymorphisms. Morphological characterisation of maca included evaluation of length, weight, shape, skin and flesh colour of hypocotyls. In yacon, we evaluated shape, colour, skin texture and flesh colour of tubers, as well as the number of roots. Preliminary results showed that maca forms low-weight hypocotyls (up to only 17.4

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27

Wipf, Daniel, Jean-Philippe Bedell, Bernard Botton, Jean Charles Munch, and François Buscot. "Polymorphism in morels: isozyme electrophoretic analysis." Canadian Journal of Microbiology 42, no. 8 (1996): 819–27. http://dx.doi.org/10.1139/m96-103.

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The aim of this study was to assess whether isozyme polymorphism in different members of the Morchellaceae could be used to improve the systematics in this fungal group and to characterize intraspecific crossings between monosporal strains in Morchella esculenta. For this purpose, isozyme electrophoretic analysis of the following enzymes was performed: glutamine synthetase, NAD–glutamate dehydrogenase, NADP–glutamate dehydrogenase, aspartate aminotransferase, malate dehydrogenase, NAD–glyceraldehyde phosphate dehydrogenase, glucose phosphate isomerase, and superoxide dismutase. The analyses al

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28

Rubino, David B. "INHERITANCE OF DIAPHORASE AND GLUCOSE-6-PHOSPHATE ISOMERASE IN EUSTOMA GRANDIFLORUM." HortScience 27, no. 6 (1992): 680a—680. http://dx.doi.org/10.21273/hortsci.27.6.680a.

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Segregating progenies from controlled pollinations of Eustoma grandiflorum Griseb. were investigated to determine the inheritance of diaphorase (DIA) and glucoses-phosphate isomerase (GPI) isozymes. Phenotypic data supported the hypotheses that DIA1 is tetrameric and is controlled by a single locus with two alleles (Dia1-1 and Dia1-2) and that GPI1 is dimeric and also is controlled by a single locus with two alleles (Gpi1-1 and Gpi1-2). Examination of isozyme phenotypes for over 70 cultivars of E. grandiflorum revealed polymorphism for DIA1 and GPI1. These isozymes may be useful for marker-ass

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29

Trujillo, Isabel, Luis Rallo, and Pere Arús. "Identifying Olive Cultivars by Isozyme Analysis." Journal of the American Society for Horticultural Science 120, no. 2 (1995): 318–24. http://dx.doi.org/10.21273/jashs.120.2.318.

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Pollen samples of 155 olive (Olea europaea L.) cultivars from different origins were analyzed to study isoenzymatic variability in five enzyme systems: alcohol dehydrogenase (ADH), esterase (EST), glucose phosphate isomerase (GPI), leucine aminopeptidase (LAP), and malic enzyme (ME) using starch gel electrophoresis. Polymorphism was observed in all of the isozyme systems. ME, GPI, EST, and LAP were the most useful systems for identification of cultivars. Different combinations of banding patterns of these systems allowed us to identify 85% of the cultivars. The remainder were separated into gr

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30

Begum, Kazi Nahida, and Sheikh Shamimul Alam. "Genetic diversity in nine chickpea (Cicer arietinum L.) varieties based on different molecular markers." Bangladesh Journal of Botany 48, no. 1 (2019): 195–203. http://dx.doi.org/10.3329/bjb.v48i1.47491.

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Анотація:

Nine varieties (BARI Chola-1 to BC-9) of chickpea (Cicer arietinum L.) were studied with respect to isozymes, SDS-PAGE, amplified DNA produced by RAPD- and SSR-markers. The varieties could be characterized by SDS-PAGE bands on the basis of their location, size and intensity. Three isozyme systems, namely acid phosphatase, esterase and peroxidase were investigated of which esterase was found to be suitable for produced distinct polymorphic bands with 57.14% diversity. Ten RAPD primers were producing 74 bands with 93.24% polymorphisms which indicated highly diverse nature. In addition to polymor

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31

Beregovoy, Vladimir H., and Dhanwant S. Gill. "Isozyme polymorphism in the sunflower moth." Journal of Heredity 77, no. 2 (1986): 101–5. http://dx.doi.org/10.1093/oxfordjournals.jhered.a110177.

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32

Sharma, Sunita, Dhanwant K. Sandhu та Parmjit S. Bagga. "Isozyme polymorphism ofβ-glucosidase inAspergillus nidulans". Biochemical Genetics 26, № 5-6 (1988): 331–42. http://dx.doi.org/10.1007/bf02401787.

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33

Sharma, Sunita, Dhanwant K. Sandhu, and Parmjit S. Bagga. "Isozyme polymorphism of?-glucosidase inAspergillus nidulans." Biochemical Genetics 26, no. 5-6 (1988): 331–42. http://dx.doi.org/10.1007/bf00554069.

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34

Singh, S., J. K. Brar, D. K. Sandhu, and A. Kaur. "Isozyme polymorphism of cellulases inAspergillus terreus." Journal of Basic Microbiology 36, no. 4 (1996): 289–96. http://dx.doi.org/10.1002/jobm.3620360412.

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Obara-Okeyo, P., Kouei Fujii, and Shunji Kako. "Enzyme Polymorphism in Cymbidium Orchid Cultivars and Inheritance of Leucine Aminopeptidase." HortScience 32, no. 7 (1997): 1267–71. http://dx.doi.org/10.21273/hortsci.32.7.1267.

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Seventy Cymbidium (Swartz.) cultivars were analyzed for isozyme variability in eight enzyme systems by starch gel electrophoresis. All systems studied [aspartate aminotransferase (AAT), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), phosphoglucomutase (PGM), glucose phosphate isomerase (GPI), triosephosphate isomerase (TPI), and shikimate dehydrogenase (SKDH)] showed polymorphism. When all enzyme systems were evaluated, 68 of the 70 Cymbidium cultivars could be distinguished. Isozymes could not distinguish betwen the cultivars Golden Star `Kumamoto' and Golden Star `Sunrise'. No cult

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36

Prince, James P., Fernando Loaiza-Figueroa, and Steven D. Tanksley. "Restriction fragment length polymorphism and genetic distance among Mexican accessions of Capsicum." Genome 35, no. 5 (1992): 726–32. http://dx.doi.org/10.1139/g92-112.

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Restriction fragment length polymorphism analysis was performed on 25 accessions of pepper (Capsicum sp.) from various regions of Mexico and on the two parents currently in use for molecular mapping. The fragments detected were used to estimate genetic distances among the accessions. A dendrogram from cluster analysis and a principal component analysis diagram of the genetic distance matrix are presented. The correlation between genetic distances measured by restriction fragment length polymorphism versus genetic distances measured by isozymes is good (R2 = 0.438, p = 0.0001). All but four acc

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DeSalle, Rob, Alan Templeton, Ikue Mori, Susan Pletscher, and J. Spencer Johnston. "Temporal and Spatial Heterogeneity of mtDNA Polymorphisms in Natural Populations of Drosophila mercatorum." Genetics 116, no. 2 (1987): 215–23. http://dx.doi.org/10.1093/genetics/116.2.215.

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ABSTRACT Restriction endonuclease analysis of mtDNA was used to examine the genetic relatedness of several geographically separated isolines of the Drosophila mercatorum subgroup. In addition, we examined the temporal and spatial distribution of two mtDNA restriction site polymorphisms produced by the enzymes BstEII and BstNI at a single locality—Kamuela, Hawaii. Due to small sample sizes of some collections and the undesirable dependance of the estimation of polymorphism frequency on its variance, an arcsin square root transformation of the frequency data was used. We also use an Fst estimato

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38

AL-Huqail, Asma A., and Ekram Abdelhaliem. "Evaluation of Genetic Variations in Maize Seedlings Exposed to Electric Field Based on Protein and DNA Markers." BioMed Research International 2015 (2015): 1–15. http://dx.doi.org/10.1155/2015/874906.

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The current study analyzed proteins and nuclear DNA of electric fields (ELF) exposed and nonexposed maize seedlings for different exposure periods using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isozymes, random amplified polymorphic DNA (RAPD), and comet assay, respectively. SDS-PAGE analysis revealed total of 46 polypeptides bands with different molecular weights ranging from 186.20 to 36.00 KDa. It generated distinctive polymorphism value of 84.62%. Leucine-aminopeptidase, peroxidase, and catalase isozymes showed the highest values of polymorphism (100%) based on

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39

Bhattacharya, Nikhil C., Sheila Bhattacharya, and Boyd R. Strain. "Isozyme Polymorphism during Rooting at Elevated CO2." HortScience 24, no. 2 (1989): 302–5. http://dx.doi.org/10.21273/hortsci.24.2.302.

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Abstract The effects of CO2 enrichment and IAA on adventitious root formation of hypocotyl cuttings of Impatiens balsamina L. ‘Camellia’ were examined. Root numbers increased significantly at 675 and 1000 µl CO2/liter compared to 350 µl liter-1. In the presence of IAA, the number of roots increased at 675 and 1000 µl CO2/liter and the effect was most pronounced with 5 µg IAA/ml at 675 µl CO2/liter. IAA-treated cuttings, compared to those in deionized water, exhibited slightly increased intensities of some of the isoperoxidases coinciding with root initiation and development in a CO2-enriched a

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40

Leuchtmann, Adrian, Orlando Petrini, Liliane E. Petrini, and George C. Carroll. "Isozyme polymorphism in six endophytic Phyllosticta species." Mycological Research 96, no. 4 (1992): 287–94. http://dx.doi.org/10.1016/s0953-7562(09)80940-4.

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41

Aradhya, Mallikarjuna K., F. T. Zee, and R. M. Manshardt. "Genetic diversity inNepheliumas revealed by isozyme polymorphism." Journal of Horticultural Science 71, no. 6 (1996): 847–57. http://dx.doi.org/10.1080/14620316.1996.11515467.

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42

MEDOUKALI, Imane, Ines BELLIL, and Douadi KHELIFI. "Morphological and Isozyme Variation in Natural Populations of the Genus Medicago L. Prospected in Northern Algeria." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 43, no. 1 (2015): 86–95. http://dx.doi.org/10.15835/nbha4319676.

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As part of the evaluation and enhancement of genetic resources, morphological and isozyme variability within and among 169 accessions, representing 14 species of the genus Medicago L. collected in northern Algeria, was assessed using twelve quantitative traits and two enzymatic systems. Phenotype frequencies were scored in six enzyme zones to determine isozyme variability within and among populations. The data analysis resolved a high level of genetic diversity. Ten morphometric characteristics contributed to the discrimination of the species. The relationship between the collection site envir

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43

Penteado, Maria Isabel de O., Pedro García, and Marcelino Pérez de la Vega. "Isozyme markers and genetic variability in three species of Centrosema (Leguminosae)." Brazilian Journal of Genetics 20, no. 3 (1997): 443–52. http://dx.doi.org/10.1590/s0100-84551997000300015.

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Isozyme patterns and their genetic control in three Centrosema species are described. Seven isozymatic systems (aspartate aminotransferase, glucose-6-phosphate isomerase, phosphoglucomutase, anodal peroxidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, and isocitrate dehydrogenase) were studied in 18 populations and several breeding lines of C. acutifolium, C. brasilianum and C. pubescens, using starch gel electrophoresis techniques. All systems, except glucose-6-phosphate isomerase, are described for the first time in these species. A total of 17 isozyme loci were scored; this re

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44

Vorsa, Nicholi, Paul S. Manos, and Maria I. van Heemstra. "Isozyme variation and inheritance in blueberry." Genome 30, no. 5 (1988): 776–81. http://dx.doi.org/10.1139/g88-125.

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Leaf tissue extracts from diploid, tetraploid, and hexaploid species of blueberry, Vaccinium section Cyanococcus, were electrophoretically analyzed for isozyme polymorphism in 12 enzyme systems. Aldolase, shikimate dehydrogenase, triose phosphate isomerase, glucose-6-phosphate dehydrogenase, phosphoglucomutase, aconitase, alcohol dehydrogenase, and aspartate aminotransferase showed activity, but banding was not clear. Four enzymes, malate dehydrogenase (MDH), phosphoglucose isomerase (PGI), 6-phosphogluconate dehydrogenase (6-PGD), and isocitrate dehydrogenase (IDH), exhibited interpretable ba

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45

Lee, K. S., D. Senadhira, and D. S. Brar. "Classification of Korean rice germplasm based on isozyme polymorphism." International Rice Research Notes 21, no. 1 (1996): 15. https://doi.org/10.5281/zenodo.6823855.

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This article 'Classification of Korean rice germplasm based on isozyme polymorphism' appeared in the International Rice Research Notes series, created by the International Rice Research Institute (IRRI) to expedite communication among scientists concerned with the development of improved technology for rice and rice-based systems. The series is a mechanism to help scientists keep each other informed of current rice research findings. The concise scientific notes are meant to encourage rice scientists to communicate with one another to obtain details on the research reported.

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PITOYO, ARI, ANGELIA ARUM PRAMETA, MARSUSI MARSUSI, SURATMAN SURATMAN, and SURANTO SURANTO. "Morphological, anatomical and isozyme variability among taro (Colocasia esculenta) accessions from southeastern part of Central Java, Indonesia." Biodiversitas Journal of Biological Diversity 19, no. 5 (2018): 1811–19. http://dx.doi.org/10.13057/biodiv/d190532.

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Pitoyo A, Prameta AA, Marsusi, Suratman, Suranto. 2018. Morphological, anatomical and isozyme variability among taro (Colocasia esculenta) accessions from southeastern part of Central Java, Indonesia. Biodiversitas 19: 1811-1819. The objective of this study was to evaluate morphological, anatomical and isozyme variability among taro accessions from southeastern part of Central Java (Indonesia). A total of 20 taro accessions were collected from a wide range of sites during field surveys. Morphological characters measurements were taken on vegetative structures such as roots, stems, leaves, and

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47

Gaafar, Reda, Mai Allam, Rasha Sabry, and Mahmoud Saker. "Molecular genetic analysis of some North African barley germplasms." Acta agriculturae Slovenica 109, no. 2 (2017): 187. http://dx.doi.org/10.14720/aas.2017.109.2.03.

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<p>Isozyme and RAPD markers were used to characterize 29 barley accessions, which were collected from North Africa. In addition, resistance gene sequences were employed to develop molecular markers using RT-PCR approach. High level of polymorphism was found with both RAPD and isozyme markers, where RAPD showed that 60 % of amplified bands were polymorphic. Peroxidase showed three polymorphic loci (7 allelic bands). Isozymes cluster analysis successfully separated the barley accessions into three geographically distinct groups. RAPD investigation demonstrated that Egyptian accessions were

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48

Barone, Ettore, Luigi Di Marco, Francesco P. Marra, and Maria Sidari. "Isozymes and Canonical Discriminant Analysis to Identify Pistachio (Pistacia vera L.) Germplasm." HortScience 31, no. 1 (1996): 134–38. http://dx.doi.org/10.21273/hortsci.31.1.134.

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Canonical discriminant analysis (CDA) of morphometric data of buds, leaves, and fruit, as well as isozyme analysis (esterase, peroxydase, and acid phosphatase) of leaf samples, were used to identify eight male pistachio selections and 10 female pistachio cultivars. According to the CDA, 77% and 93% of the total variance was summarized by the first three canonical discriminant functions for the female and male selections, respectively. Fruit characteristics, particularly fruit fresh and dry weights and fruit length, accounted for most of the discriminatory power for the female cultivars, while

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49

Kagan-Zur, Varda, Yosef Mizrahi, Dan Zamir, and Nir Navot. "A Tomato Triploid Hybrid Whose Double Genome Parent Is the Male." Journal of the American Society for Horticultural Science 116, no. 2 (1991): 342–45. http://dx.doi.org/10.21273/jashs.116.2.342.

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A spontaneous tomato (Lycopersicon esculentum Mill.) triploid hybrid was analyzed by isozyme and restriction fragment length polymorphism profiles. The double chromosome complement donor was shown to be the male parent, contrary to the prevailing hypothesis.

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50

Simcox, K. D. "Comparison of Isozyme Polymorphism in Races ofCochliobolus carbonum." Phytopathology 82, no. 6 (1992): 621. http://dx.doi.org/10.1094/phyto-82-621.

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