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Готові списки джерел за темами / Membrane destabilization / Статті в журналах

Щоб переглянути інші типи публікацій з цієї теми, перейдіть за посиланням: Membrane destabilization.

Статті в журналах з теми "Membrane destabilization"

Автор: Grafiati

Опубліковано: 10 березня 2023

Оновлено: 31 липня 2025

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1

Sun, Jan, Elena E. Pohl, Oxana O. Krylova, et al. "Membrane destabilization by ricin." European Biophysics Journal 33, no. 7 (2004): 572–79. http://dx.doi.org/10.1007/s00249-004-0400-9.

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2

Storm, G., and E. Wagner. "Membrane destabilization for improved cystolic delivery." Advanced Drug Delivery Reviews 38, no. 3 (1999): 195. http://dx.doi.org/10.1016/s0169-409x(99)00028-9.

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3

Schutzbach, John S., and John W. Jensen. "Bilayer membrane destabilization induced by dolichylphosphate." Chemistry and Physics of Lipids 51, no. 3-4 (1989): 213–18. http://dx.doi.org/10.1016/0009-3084(89)90008-x.

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4

Luján, H. D., and D. H. Bronia. "Intermembrane lipid transfer duringTrypanosoma cruzi-induced erythrocyte membrane destabilization." Parasitology 108, no. 3 (1994): 323–34. http://dx.doi.org/10.1017/s0031182000076162.

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SUMMARYThe ability ofTrypanosoma cruzito induce erythrocyte membrane destabilizationin vitrowas studied. Epimastigote forms adhered to human erythrocytes and caused fusion or lysis of the red cells, depending on the conditions of the interaction. Red cells were fused in the presence of calcium, while haemolysis was induced in the absence of the cation. Dextran 60 C facilitated fusion but delayed lysis. Optimum pH and temperature for fusion were 7·4 and 37 °C, respectively. Lipid alterations were produced in the plasma membrane of the red cell during the interaction with the parasite. A Ca2+-in

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5

Harper, Sandra L., Sira Sriswasdi, Hsin-Yao Tang, Massimiliano Gaetani, Patrick G. Gallagher та David W. Speicher. "The common hereditary elliptocytosis-associated α-spectrin L260P mutation perturbs erythrocyte membranes by stabilizing spectrin in the closed dimer conformation". Blood 122, № 17 (2013): 3045–53. http://dx.doi.org/10.1182/blood-2013-02-487702.

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Key Points The common HE mutation αL260P reduces spectrin tetramer links between junctional complexes in red cell membranes by favoring closed dimers. Favoring closed spectrin dimer formation is a new mechanism of red cell membrane destabilization by hereditary anemia mutations.

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6

Jin, Seok Min, Michael Lazarou, Chunxin Wang, Lesley A. Kane, Derek P. Narendra, and Richard J. Youle. "Mitochondrial membrane potential regulates PINK1 import and proteolytic destabilization by PARL." Journal of Cell Biology 191, no. 5 (2010): 933–42. http://dx.doi.org/10.1083/jcb.201008084.

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PINK1 is a mitochondrial kinase mutated in some familial cases of Parkinson’s disease. It has been found to work in the same pathway as the E3 ligase Parkin in the maintenance of flight muscles and dopaminergic neurons in Drosophila melanogaster and to recruit cytosolic Parkin to mitochondria to mediate mitophagy in mammalian cells. Although PINK1 has a predicted mitochondrial import sequence, its cellular and submitochondrial localization remains unclear in part because it is rapidly degraded. In this study, we report that the mitochondrial inner membrane rhomboid protease presenilin-associat

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7

Steponkus, Peter. "Membrane destabilization resulting from freeze-induced dehydration." Cryobiology 24, no. 6 (1987): 555. http://dx.doi.org/10.1016/0011-2240(87)90096-4.

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8

Morales, Joselyn, та Suren A. Tatulian. "Membrane Destabilization by Alzheimer's Amyloid β Peptide". Biophysical Journal 104, № 2 (2013): 239a—240a. http://dx.doi.org/10.1016/j.bpj.2012.11.1351.

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9

Voisine, Richard, Claude Willemot, and Louis Vézina. "MICROSOMAL MEMBRANE CHANGES IN IRRADIATED CAULIFLOWER DURING STORAGE." HortScience 25, no. 9 (1990): 1086d—1086. http://dx.doi.org/10.21273/hortsci.25.9.1086d.

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Cauliflowers (Brassica oleracea) were irradiated at 0, 2, and 4 kGy and stored 8 days at 13°C. Development of yellow color and browning of the in florescence, increase in membrane electrolyte leakage and reduction of protein recovery in microsomal membranes were observed over the storage period. Changes in membrane free fatty acids, lipid phosphorus content, peroxydation level, and fatty acid composition of polar lipids also occurred. These results indicate an important modification of cellular membranes. The direct effect of gamma rays on membrane lipids via free radical production and subseq

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10

Lau, Wai Leung, David S. Ege, James D. Lear, Daniel A. Hammer, and William F. DeGrado. "Oligomerization of Fusogenic Peptides Promotes Membrane Fusion by Enhancing Membrane Destabilization." Biophysical Journal 86, no. 1 (2004): 272–84. http://dx.doi.org/10.1016/s0006-3495(04)74103-x.

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11

Pascual, Roberto, Miguel R. Moreno, and José Villalaín. "A Peptide Pertaining to the Loop Segment of Human Immunodeficiency Virus gp41 Binds and Interacts with Model Biomembranes: Implications for the Fusion Mechanism." Journal of Virology 79, no. 8 (2005): 5142–52. http://dx.doi.org/10.1128/jvi.79.8.5142-5152.2005.

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ABSTRACT The human immunodeficiency virus gp41 envelope protein mediates the entry of the virus into the target cell by promoting membrane fusion. In order to gain new insights into the viral fusion mechanism, we studied a 35-residue peptide pertaining to the loop domain of gp41, both in solution and membrane bound, by using infrared and fluorescence spectroscopy. We show here that the peptide, which has a membrane-interacting surface, binds and interacts with phospholipid model membranes and tends to aggregate in the presence of a membranous medium and induce the leakage of vesicle contents.

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12

PRACHAYASITTIKUL, Virapong, Chartchalerm ISARANKURA-NA-AYUDHYA, Tanawut TANTIMONGCOLWAT, Chanin NANTASENAMAT, and Hans-Joachim GALLA. "EDTA-induced Membrane Fluidization and Destabilization: Biophysical Studies on Artificial Lipid Membranes." Acta Biochimica et Biophysica Sinica 39, no. 11 (2007): 901–13. http://dx.doi.org/10.1111/j.1745-7270.2007.00350.x.

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13

Eriksson, Ida, Linda Vainikka, Hans Lennart Persson, and Karin Öllinger. "Real-Time Monitoring of Lysosomal Membrane Permeabilization Using Acridine Orange." Methods and Protocols 6, no. 4 (2023): 72. http://dx.doi.org/10.3390/mps6040072.

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Loss of lysosomal membrane integrity results in leakage of lysosomal hydrolases to the cytosol which might harm cell function and induce cell death. Destabilization of lysosomes often precede apoptotic or necrotic cell death and occur during both physiological and pathological conditions. The weak base acridine orange readily enters cells and accumulates in the acidic environment of lysosomes. Vital staining with acridine orange is a well-proven technique to observe lysosomal destabilization using fluorescence microscopy and flow cytometry. These analyses are, however, time consuming and only

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14

Brasseur, Robert. "Tilted peptides: a motif for membrane destabilization (Hypothesis)." Molecular Membrane Biology 17, no. 1 (2000): 31–40. http://dx.doi.org/10.1080/096876800294461.

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15

Muga, Arturo, Henry H. Mantsch, and Witold K. Surewicz. "Membrane binding induces destabilization of cytochrome c structure." Biochemistry 30, no. 29 (1991): 7219–24. http://dx.doi.org/10.1021/bi00243a025.

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16

Lonez, Caroline, Marc F. Lensink, Bouna-Moussa Tandia, Michel Vandenbranden, and Jean-Marie Ruysschaert. "Cationic Lipids: from Membrane Destabilization to Cell Signaling." Biophysical Journal 98, no. 3 (2010): 435a. http://dx.doi.org/10.1016/j.bpj.2009.12.2362.

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17

Lee, S., N. Jang, and Y. Watanabe. "Effect of residual ozone on membrane fouling reduction in ozone resisting microfiltration (MF) membrane system." Water Science and Technology 50, no. 12 (2004): 287–92. http://dx.doi.org/10.2166/wst.2004.0725.

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The effect of residual ozone on reducing the membrane fouling was investigated using ozone resisting microfiltration membrane. It was found out that the fouling was reduced effectively by maintaining residual ozone in the membrane module. To clarify the reason why the residual ozone reduces the membrane fouling, research was focused on the molecular degradation reaction and particle destabilization reaction induced by residual ozone. The major reason of membrane fouling reduction was attributed to the reduction of reversible resistance induced by the cake layer. The reversible resistance was r

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18

Hazlett, Karsten R. O., David L. Cox, Marc Decaffmeyer, et al. "TP0453, a Concealed Outer Membrane Protein of Treponema pallidum, Enhances Membrane Permeability." Journal of Bacteriology 187, no. 18 (2005): 6499–508. http://dx.doi.org/10.1128/jb.187.18.6499-6508.2005.

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ABSTRACT The outer membrane of Treponema pallidum, the noncultivable agent of venereal syphilis, contains a paucity of protein(s) which has yet to be definitively identified. In contrast, the outer membranes of gram-negative bacteria contain abundant immunogenic membrane-spanning β-barrel proteins mainly involved in nutrient transport. The absence of orthologs of gram-negative porins and outer membrane nutrient-specific transporters in the T. pallidum genome predicts that nutrient transport across the outer membrane must differ fundamentally in T. pallidum and gram-negative bacteria. Here we d

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19

Novak, Sara, Damjana Drobne, and anja menard. "Prolonged feeding of terrestrial isopod (Porcellio scaber, Isopoda, Crustacea) on TiO2 nanoparicles. Absence of toxic effect." ZooKeys 176 (March 20, 2012): 261–73. https://doi.org/10.3897/zookeys.176.2463.

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Nanoparticles of titanium dioxide are one of most widely used nanomaterials in different products in everyday use and in industry, but very little is known about their effects on non- target cells and tissues. Terrestrial isopods were exposed to food dosed with nano-TiO2 to give final nominal concentration 1000 and 2000 µg TiO2/g dry weight of food. The effects of ingested nano-TiO2 on the model invertebrate <i>Porcellio scaber</i> (Isopoda, Crustacea) after short-term (3 and 7 days) and prolonged (14 and 28 days) dietary exposure was assessed by conventional toxicity measures such as feeding

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20

Haikou, Maria N., Paraskevi Zagana, Panayiotis V. Ioannou, and Sophia G. Antimisiaris. "Arsonoliposome Interaction with Thiols: Effect of Pegylation and Arsonolipid Content of Arsonoliposomes on Their Integrity During Incubation in Glutathione." Journal of Nanoscience and Nanotechnology 6, no. 9 (2006): 2974–78. http://dx.doi.org/10.1166/jnn.2006.422.

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Increased toxicity of arsonoliposomes towards cancer cells may be attributed to interaction between arsonolipids and cellular thiols which, would result in reduction of As(V) to the more toxic As(III). Cancer cells with high thiol contents may thus be more sensitive to arsonoliposomes, providing that the arsonolipid molecules that are incorporated in the liposome membrane can interact with thiol-containing compounds. For examination of this possibility we investigate the effect of incubating various compositions of arsonoliposomes with glutathione, on their integrity. If glutathione does inter

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21

Chang, Rupert, Shlomo Nir, and Francis R. Poulain. "Analysis of binding and membrane destabilization of phospholipid membranes by surfactant apoprotein B." Biochimica et Biophysica Acta (BBA) - Biomembranes 1371, no. 2 (1998): 254–64. http://dx.doi.org/10.1016/s0005-2736(98)00031-5.

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22

Boya, Patricia, Karine Andreau, Delphine Poncet, et al. "Lysosomal Membrane Permeabilization Induces Cell Death in a Mitochondrion-dependent Fashion." Journal of Experimental Medicine 197, no. 10 (2003): 1323–34. http://dx.doi.org/10.1084/jem.20021952.

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A number of diseases are due to lysosomal destabilization, which results in damaging cell loss. To investigate the mechanisms of lysosomal cell death, we characterized the cytotoxic action of two widely used quinolone antibiotics: ciprofloxacin (CPX) or norfloxacin (NFX). CPX or NFX plus UV light (NFX*) induce lysosomal membrane permeabilization (LMP), as detected by the release of cathepsins from lysosomes. Inhibition of the lysosomal accumulation of CPX or NFX suppresses their capacity to induce LMP and to kill cells. CPX- or NFX-triggered LMP results in caspase-independent cell death, with

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23

Sánchez-Villalba, Esther, María Elena Arias, Fabiola Zambrano, Pía Loren, and Ricardo Felmer. "Improved exogenous DNA uptake in bovine spermatozoa and gene expression in embryos using membrane destabilizing agents in ICSI-SMGT." Zygote 26, no. 1 (2018): 104–9. http://dx.doi.org/10.1017/s0967199417000727.

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SummarySperm-mediated gene transfer (SMGT) is a simple, fast, and economical biotechnological tool for producing transgenic animals. However, transgene expression with this technique in bovine embryos is still inefficient due to low uptake and binding of exogenous DNA in spermatozoa. The present study evaluated the effects of sperm membrane destabilization on the binding capacity, location and quantity of bound exogenous DNA in cryopreserved bovine spermatozoa using Triton X-100 (TX-100), lysolecithin (LL) and sodium hydroxide (NaOH). Effects of these treatments were also evaluated by intracyt

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24

Tian, P., J. M. Ball, C. Q. Zeng, and M. K. Estes. "The rotavirus nonstructural glycoprotein NSP4 possesses membrane destabilization activity." Journal of virology 70, no. 10 (1996): 6973–81. http://dx.doi.org/10.1128/jvi.70.10.6973-6981.1996.

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25

Silberstein, Anatoli, Tajib Mirzabekov, W. French Anderson, and Yanina Rozenberg. "Membrane destabilization assay based on potassium release from liposomes." Biochimica et Biophysica Acta (BBA) - Biomembranes 1461, no. 1 (1999): 103–12. http://dx.doi.org/10.1016/s0005-2736(99)00152-2.

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26

Malekkhaiat Häffner, S., E. Parra-Ortiz, M. W. A. Skoda, T. Saerbeck, K. L. Browning, and M. Malmsten. "Composition effects on photooxidative membrane destabilization by TiO2 nanoparticles." Journal of Colloid and Interface Science 584 (February 2021): 19–33. http://dx.doi.org/10.1016/j.jcis.2020.09.046.

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27

Shin, Sungmin, Hyunhyuk Tae, Soohyun Park, and Nam-Joon Cho. "Lipid Membrane Remodeling by the Micellar Aggregation of Long-Chain Unsaturated Fatty Acids for Sustainable Antimicrobial Strategies." International Journal of Molecular Sciences 24, no. 11 (2023): 9639. http://dx.doi.org/10.3390/ijms24119639.

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Antimicrobial fatty acids derived from natural sources and renewable feedstocks are promising surface-active substances with a wide range of applications. Their ability to target bacterial membrane in multiple mechanisms offers a promising antimicrobial approach for combating bacterial infections and preventing the development of drug-resistant strains, and it provides a sustainable strategy that aligns with growing environmental awareness compared to their synthetic counterparts. However, the interaction and destabilization of bacterial cell membranes by these amphiphilic compounds are not ye

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28

POLYANSKY, ANTON A., PAVEL E. VOLYNSKY, and ROMAN G. EFREMOV. "COMPUTER SIMULATIONS OF MEMBRANE-LYTIC PEPTIDES: PERSPECTIVES IN DRUG DESIGN." Journal of Bioinformatics and Computational Biology 05, no. 02b (2007): 611–26. http://dx.doi.org/10.1142/s0219720007002783.

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Structure activity relationships were investigated for membrane-lytic peptides (MLP) Ltc1 and Ltc2a from the latarcin family. The peptides were studied via long-term molecular dynamics (MD) simulations in different membrane environments (detergent micelles, mixed lipid bilayers mimiking eukaryotic and bacterial membranes). The calculated structure of Ltc2a in sodium dodecyl sulfate micelle agrees well with the data obtained by 1H-NMR spectroscopy. This validates the applied modeling approach. The binding mode of MLPs is governed by several factors: (i) the membrane surface curvature; (ii) the

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29

Monnier, Noadya, Aurélien Furlan, Sébastien Buchoux, et al. "Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies." International Journal of Molecular Sciences 20, no. 5 (2019): 1009. http://dx.doi.org/10.3390/ijms20051009.

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Rhamnolipids (RLs) are potential biocontrol agents for crop culture protection. Their mode of action has been proposed as dual, combining plant protection activation and antifungal activities. The present work focuses on the interaction of natural RLs with plant and fungi membrane models at the molecular scale. Representative models were constructed and the interaction with RLs was studied by Fourier transform infrared (FTIR) and deuterium nuclear magnetic resonance (2H NMR) spectroscopic measurements. Molecular dynamic (MD) simulations were performed to investigate RL insertion in lipid bilay

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30

An, Xiuli, Marcela Salomao, Xinhua Guo, Walter Gratzer, and Narla Mohandas. "Tropomyosin modulates erythrocyte membrane stability." Blood 109, no. 3 (2006): 1284–88. http://dx.doi.org/10.1182/blood-2006-07-036954.

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Abstract The ternary complex of spectrin, actin, and 4.1R (human erythrocyte protein 4.1) defines the nodes of the erythrocyte membrane skeletal network and is inseparable from membrane stability under mechanical stress. These junctions also contain tropomyosin (TM) and the other actin-binding proteins, adducin, protein 4.9, tropomodulin, and a small proportion of capZ, the functions of which are poorly defined. Here, we have examined the consequences of selective elimination of TM from the membrane. We have shown that the mechanical stability of the membranes of resealed ghosts devoid of TM i

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31

Richter, Christoph. "Oxidative Stress, Mitochondria, and Apoptosis." Restorative Neurology and Neuroscience 12, no. 2-3 (1998): 59–62. https://doi.org/10.3233/rnn-1998-00019.

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This article emphasizes the importance of mitochondria, the cellular ATP level, and the liberation of certain mitochondrial proteins for the execution phase of apoptosis. Destabilization of mitochondria results in release of these proteins. Oxidative stress and altered cellular Ca2+ homeostatis, considered to be mediators of apoptosis, synergistically decrease the mitochondrial membrane potential and lower the cellular ATP level. Conversely, stabilization of the mitochondrial membrane potential, e.g., by the protooncogene bcl-2, prevents cell death. An important process underlying mitochondria

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32

Mangalanathan, Malathi, Tamiloli Devendhiran, Saraswathi Uthamaramasamy, et al. "Membrane stabilization effect and histological changes in the heart in experimental myocardial rats with Zanthoxulym armatum fruit." South Asian Journal of Engineering and Technology 8, no. 1 (2019): 19–26. http://dx.doi.org/10.26524/sajet190805.

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Membrane bound adenosine triphosphatases (ATPases) shed a massive function into the contraction and relaxation of the heart muscle via keeping the normal ion levels within the myocyte. The current study aims to assess the potency of Zanthoxylum armatum (Z. armatum) fruit on membrane bound ATPases and ions in Isoproterenol (ISO) induced myocardial infracted rats. The hydroethanolic extract of Z. armatum fruit was administered at a dose of 200 and 400mg/kg body weight for 30 days to male Wistar albino rats. On 28th and 29th day, ISO (8.5mg/100g body weight) used to be administered to induce myoc

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33

Gaussier, Hélène, Thierry Lefèvre, and Muriel Subirade. "Binding of Pediocin PA-1 with Anionic Lipid Induces Model Membrane Destabilization." Applied and Environmental Microbiology 69, no. 11 (2003): 6777–84. http://dx.doi.org/10.1128/aem.69.11.6777-6784.2003.

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ABSTRACT To obtain molecular insights into the action mode of antimicrobial activity of pediocin PA-1, the interactions between this bacteriocin and dimyristoylphosphatidylcholine (DMPC) or dimyristoylphosphatidylglycerol (DMPG) model membranes have been investigated in D2O at pD 6 by Fourier transform infrared spectroscopy. The interactions were monitored with respect to alteration of the secondary structure of pediocin, as registered by the amide I′ band, and phospholipid conformation, as revealed by the methylene νs(CH2) and carbonyl ν(C═;O) stretching vibrations. The results show that no i

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34

Chen, Siyuan, Shiqi Wang, Michal Kopytynski, Marie Bachelet, and Rongjun Chen. "Membrane-Anchoring, Comb-Like Pseudopeptides for Efficient, pH-Mediated Membrane Destabilization and Intracellular Delivery." ACS Applied Materials & Interfaces 9, no. 9 (2017): 8021–29. http://dx.doi.org/10.1021/acsami.7b00498.

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35

Mason, A. James, Arnaud Marquette, and Burkhard Bechinger. "Zwitterionic Phospholipids and Sterols Modulate Antimicrobial Peptide-Induced Membrane Destabilization." Biophysical Journal 93, no. 12 (2007): 4289–99. http://dx.doi.org/10.1529/biophysj.107.116681.

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36

Lobo, ReemaOrison, BK Chandrasekhar Sagar, and ChandrakalaK Shenoy. "Bio-tea prevents membrane destabilization during Isoproterenol-induced myocardial injury." Journal of Microscopy and Ultrastructure 5, no. 3 (2017): 146. http://dx.doi.org/10.1016/j.jmau.2016.09.001.

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37

Düzgüneş, Nejat, and Sergei A. Shavnin. "Membrane destabilization by N-terminal peptides of viral envelope proteins." Journal of Membrane Biology 128, no. 1 (1992): 71–80. http://dx.doi.org/10.1007/bf00231872.

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38

Tkeshelashvili, L. K., O. V. Khuiusauri, and K. D. Tsakadze. "On the mechanism of erythrocyte membrane destabilization by copper ions." Journal of Inorganic Biochemistry 36, no. 3-4 (1989): 349. http://dx.doi.org/10.1016/0162-0134(89)84590-8.

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39

Louise, Chopinet, Dague Etienne, and Rols Marie-Pierre. "AFM sensing cortical actin cytoskeleton destabilization during plasma membrane electropermeabilization." Cytoskeleton 71, no. 10 (2014): 587–94. http://dx.doi.org/10.1002/cm.21194.

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40

Johnson, RM, Y. Ravindranath, M. el-Alfy, and G. Jr Goyette. "Oxidant damage to erythrocyte membrane in glucose-6-phosphate dehydrogenase deficiency: correlation with in vivo reduced glutathione concentration and membrane protein oxidation." Blood 83, no. 4 (1994): 1117–23. http://dx.doi.org/10.1182/blood.v83.4.1117.1117.

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Abstract Chronic nonspherocytic hemolytic anemia has been observed in a recently described glucose-6-phosphate dehydrogenase (G6PD) variant, G6PDWayne. The mechanical properties of these erythrocytes and other G6PD variants were examined. The deformability of G6PD-deficient erythrocytes was normal, as determined by osmotic scan ektacytometry, and was not significantly affected by hemolytic crisis. In the common varieties of G6PD deficiency, the mechanical stability of the red blood cell (RBC) membrane was greater than normal, but G6PDWayne membranes were abnormally susceptible to shear-induced

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41

Johnson, RM, Y. Ravindranath, M. el-Alfy, and G. Jr Goyette. "Oxidant damage to erythrocyte membrane in glucose-6-phosphate dehydrogenase deficiency: correlation with in vivo reduced glutathione concentration and membrane protein oxidation." Blood 83, no. 4 (1994): 1117–23. http://dx.doi.org/10.1182/blood.v83.4.1117.bloodjournal8341117.

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Chronic nonspherocytic hemolytic anemia has been observed in a recently described glucose-6-phosphate dehydrogenase (G6PD) variant, G6PDWayne. The mechanical properties of these erythrocytes and other G6PD variants were examined. The deformability of G6PD-deficient erythrocytes was normal, as determined by osmotic scan ektacytometry, and was not significantly affected by hemolytic crisis. In the common varieties of G6PD deficiency, the mechanical stability of the red blood cell (RBC) membrane was greater than normal, but G6PDWayne membranes were abnormally susceptible to shear-induced fragment

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42

Tadros, Wael, Simon A. Houston, Arash Bashirullah, et al. "Regulation of Maternal Transcript Destabilization During Egg Activation in Drosophila." Genetics 164, no. 3 (2003): 989–1001. http://dx.doi.org/10.1093/genetics/164.3.989.

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AbstractIn animals, the transfer of developmental control from maternal RNAs and proteins to zygotically derived products occurs at the midblastula transition. This is accompanied by the destabilization of a subset of maternal transcripts. In Drosophila, maternal transcript destabilization occurs in the absence of fertilization and requires specific cis-acting instability elements. We show here that egg activation is necessary and sufficient to trigger transcript destabilization. We have identified 13 maternal-effect lethal loci that, when mutated, result in failure of maternal transcript degr

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43

Carretero, Gustavo Penteado Battesini, Greice Kelle Viegas Saraiva, Magali Aparecida Rodrigues, et al. "Naphthalimide-Containing BP100 Leads to Higher Model Membranes Interactions and Antimicrobial Activity." Biomolecules 11, no. 4 (2021): 542. http://dx.doi.org/10.3390/biom11040542.

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In a large variety of organisms, antimicrobial peptides (AMPs) are primary defenses against pathogens. BP100 (KKLFKKILKYL-NH2), a short, synthetic, cationic AMP, is active against bacteria and displays low toxicity towards eukaryotic cells. BP100 acquires a α-helical conformation upon interaction with membranes and increases membrane permeability. Despite the volume of information available, the action mechanism of BP100, the selectivity of its biological effects, and possible applications are far from consensual. Our group synthesized a fluorescent BP100 analogue containing naphthalimide link

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44

Vahidi, Siavash, Yumin Bi, Stanley D. Dunn та Lars Konermann. "Load-dependent destabilization of the γ-rotor shaft in FOF1 ATP synthase revealed by hydrogen/deuterium-exchange mass spectrometry". Proceedings of the National Academy of Sciences 113, № 9 (2016): 2412–17. http://dx.doi.org/10.1073/pnas.1520464113.

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FoF1 is a membrane-bound molecular motor that uses proton-motive force (PMF) to drive the synthesis of ATP from ADP and Pi. Reverse operation generates PMF via ATP hydrolysis. Catalysis in either direction involves rotation of the γε shaft that connects the α3β3 head and the membrane-anchored cn ring. X-ray crystallography and other techniques have provided insights into the structure and function of FoF1 subcomplexes. However, interrogating the conformational dynamics of intact membrane-bound FoF1 during rotational catalysis has proven to be difficult. Here, we use hydrogen/deuterium exchange

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45

Wang, Jie, Klaas Yperman, Peter Grones, et al. "Conditional destabilization of the TPLATE complex impairs endocytic internalization." Proceedings of the National Academy of Sciences 118, no. 15 (2021): e2023456118. http://dx.doi.org/10.1073/pnas.2023456118.

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In plants, endocytosis is essential for many developmental and physiological processes, including regulation of growth and development, hormone perception, nutrient uptake, and defense against pathogens. Our toolbox to modulate this process is, however, rather limited. Here, we report a conditional tool to impair endocytosis. We generated a partially functional TPLATE allele by substituting the most conserved domain of the TPLATE subunit of the endocytic TPLATE complex (TPC). This substitution destabilizes TPC and dampens the efficiency of endocytosis. Short-term heat treatment increases TPC d

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46

Allard, A., M. Bouzid, T. Betz, et al. "Actin modulates shape and mechanics of tubular membranes." Science Advances 6, no. 17 (2020): eaaz3050. http://dx.doi.org/10.1126/sciadv.aaz3050.

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The actin cytoskeleton shapes cells and also organizes internal membranous compartments. In particular, it interacts with membranes for intracellular transport of material in mammalian cells, yeast, or plant cells. Tubular membrane intermediates, pulled along microtubule tracks, are formed during this process and destabilize into vesicles. While the role of actin in tubule destabilization through scission is suggested, literature also provides examples of actin-mediated stabilization of membranous structures. To directly address this apparent contradiction, we mimic the geometry of tubular int

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47

Delgado, N. M., M. L. Sánchez-Vázquez, O. Hernández, and R. Reyes. "Correlation Between Sperm Membrane Destabilization by Heparin and Aniline Blue Staining as Membrane Integrity Index." Archives of Andrology 40, no. 2 (1998): 147–52. http://dx.doi.org/10.3109/01485019808987937.

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48

Harrison, RA. "Capacitation mechanisms, and the role of capacitation as seen in eutherian mammals." Reproduction, Fertility and Development 8, no. 4 (1996): 581. http://dx.doi.org/10.1071/rd9960581.

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Capacitation, the process whereby spermatozoa are rendered capable of interacting with and fertilizing the egg, was discovered more than 40 years ago. However, our understanding of it is still far from satisfactory. Several factors conspire to obfuscate studies of capacitation mechanisms: the inherent functional heterogeneity of sperm populations, the range of functions used as parameters of capacitation (whence the endpoint of the process has become conceptually uncertain), and the several profound differences between model in vitro fertilization (IVF) systems and the situation in vivo in the

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49

El-Assaad, Fatima, Valery Combes, Georges Emile Raymond Grau, and Ronan Jambou. "Potential Efficacy of Citicoline as Adjunct Therapy in Treatment of Cerebral Malaria." Antimicrobial Agents and Chemotherapy 58, no. 1 (2013): 602–5. http://dx.doi.org/10.1128/aac.02591-12.

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ABSTRACTCerebral malaria (CM) is characterized by a dysregulated immune response that results in endothelial membrane destabilization and increased microparticle (MP) production. Citicoline (CTC) is a membrane stabilizer used for the treatment of neurological disorders. We evaluated the efficacy of CTC as adjunct therapy to aid recovery from experimental CM. We show that CTC reduces MP productionin vitro; in combination with artesunatein vivo, confers partial protection against CM; and prolongs survival.

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50

Abdel-Hamid, N. M., M. A. El-Moselhy, and A. El-Baz. "Hepatocyte Lysosomal Membrane Stabilization by Olive Leaves against Chemically Induced Hepatocellular Neoplasia in Rats." International Journal of Hepatology 2011 (2011): 1–6. http://dx.doi.org/10.4061/2011/736581.

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Extensive efforts are exerted looking for safe and effective chemotherapy for hepatocellular carcinoma (HCC). Specific and sensitive early biomarkers for HCC still in query. Present work to study proteolytic activity and lysosomal membrane integrity by hepatocarcinogen, trichloroacetic acid (TCA), in Wistar rats against aqueous olive leaf extract (AOLE).TCA showed neoplastic changes as oval- or irregular-shaped hepatocytes and transformed, vesiculated, and binucleated liver cells. The nuclei were pleomorphic and hyperchromatic. These changes were considerably reduced by AOLE. The results added

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