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Статті в журналах з теми "Minibioreactor"

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Автор: Grafiati
Опубліковано: 13 вересня 2021
Оновлено: 18 лютого 2022

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1

Vallejos, Jose R., Yordan Kostov, Arun Ram, Joseph A. French, Mark R. Marten, and Govind Rao. "Optical analysis of liquid mixing in a minibioreactor." Biotechnology and Bioengineering 93, no. 5 (2006): 906–11. http://dx.doi.org/10.1002/bit.20785.

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2

Kumar, Arul, and M. N. Sivakumar. "Different Mechanized Minibioreactor Framework for Multifunctional Screening in Biotechnology." Research Journal of Pharmacy and Technology 11, no. 12 (2018): 5379. http://dx.doi.org/10.5958/0974-360x.2018.00981.2.

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3

Gu, Man Bock, Geun Cheol Gil, and Joong Hyun Kim. "A two-stage minibioreactor system for continuous toxicity monitoring." Biosensors and Bioelectronics 14, no. 4 (April 1999): 355–61. http://dx.doi.org/10.1016/s0956-5663(99)00017-2.

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4

Rodriguez, Joaquín V., María Dolores Pizarro, Angel L. Scandizzi, Edgardo E. Guibert, Luciana L. Almada, and María E. Mamprin. "Construction and Performance of a Minibioreactor Suitable as Experimental Bioartificial Liver." Artificial Organs 32, no. 4 (April 2008): 323–28. http://dx.doi.org/10.1111/j.1525-1594.2007.00435.x.

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5

Soley, A., A. Fontova, J. Gálvez, E. Sarró, M. Lecina, R. Bragós, J. J. Cairó, and F. Gòdia. "Development of a simple disposable six minibioreactor system for suspension mammalian cell culture." Process Biochemistry 47, no. 4 (April 2012): 597–605. http://dx.doi.org/10.1016/j.procbio.2011.12.022.

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6

Lee, J. H., C. H. Song, B. C. Kim, and M. B. Gu. "Application of a multi-channel system for continuous monitoring and an early warning system." Water Science and Technology 53, no. 4-5 (February 1, 2006): 341–46. http://dx.doi.org/10.2166/wst.2006.139.

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A multi-channel continuous toxicity monitoring system developed in our laboratory, based on two-stage mini-bioreactors, was successfully implemented in the form of computer-based data acquisition. The multi-channel system consists of a series of a two-stage minibioreactor systems connected by a fiber optic probe to a luminometer, and uses genetically engineered bioluminescent bacteria for the detection of the potential toxicity from the soluble chemicals. This system can be stably and continuously operated due to the separation of the culture reactor from the test reactor and accomplish easy and long-term monitoring without system shut down by abrupt inflows of severe polluting chemicals. Four different recombinant bioluminescent bacteria were used in different channels so that the modes of the samples toxicities can be reasonably identified and evaluated based upon the response signature of each channel. The bioluminescent signatures were delivered from four channels by switching one at once, while the data is automatically logged to an IBM compatible computer. We also achieved the enhancement of the system through the manipulation of the dilution rate and the use of thermo-lux fusion strains. Finally, this system is now being implemented to a drinking water reservoir and river for remote sensing as an early warning system.
7

Zou, Xiang, Xia Guo, and Min Sun. "pH control strategy in a shaken minibioreactor for polysaccharide production by medicinal mushroom Phellinus linteus and its anti-hyperlipemia activity." Bioprocess and Biosystems Engineering 32, no. 2 (June 28, 2008): 277–81. http://dx.doi.org/10.1007/s00449-008-0241-5.

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8

Kumar, Sathish, Christoph Wittmann, and Elmar Heinzle. "Review: Minibioreactors." Biotechnology Letters 26, no. 1 (January 2004): 1–10. http://dx.doi.org/10.1023/b:bile.0000009469.69116.03.

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9

Aboka, Fredrick O., Wouter A. Winden, Mashego M. Reginald, Walter M. Gulik, Marco Berg, Arthur Oudshoorn, and Joseph J. Heijnen. "Identification of informative metabolic responses using a minibioreactor: a small step change in the glucose supply rate creates a large metabolic response in Saccharomyces cerevisiae." Yeast 29, no. 3-4 (March 2012): 95–110. http://dx.doi.org/10.1002/yea.2892.

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10

Coufalík, Pavel, Peter Váczi, Ondřej Zvěřina, Kateřina Trnková, Kateřina Skácelová, Miloš Barták, and Josef Komárek. "Nitrate and ammonium ions contents in field minibioreactors with Antarctic freshwater autotrophs." Czech Polar Reports 3, no. 2 (June 1, 2013): 196–207. http://dx.doi.org/10.5817/cpr2013-2-20.

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The content of nitrate and ammonium ions in aquatic environment is an important factor in the development of microorganisms colonies especially in low-nutrient environment. In this study, field experiments using small-volume minibioreactors were performed directly on the James Ross Island in Antarctica in order to describe changes in nitrate and ammonium ions contents in the Antarctic environment The ion concentrations in minibioreactors with local freshwater autotrophs was monitored for increased eutro-phication conditions. The content of nitrogen forms was determined in water samples taken from the minibioreactors regularly. Samples were taken to the laboratory of the Johann Gregor Mendel station where nitrogen content was evaluated using ion-selective electrodes. Furthermore, the freshwater autotrophs was subjected to basic taxonomic study. Closed system of the minibioreactors allowed the monitoring of nitrogen speciation changes which take place in the environment. These changes can be attributed to both the biological activity of microorganisms and external conditions. Increased eutrophication of water did not induce a rapid development of the freshwater autotrophs.
11

Anane, E., B. Haby, S. Hans, F. Glauche, P. Neubauer, and M. N. Cruz Bournazou. "Scaling down further: Model-based scale-down studies in minibioreactors." New Biotechnology 44 (October 2018): S60—S61. http://dx.doi.org/10.1016/j.nbt.2018.05.049.

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12

Weiss, Bettina, Stefan Emberger, Tobias Maucher, and Alexander Komitakis. "Automation of minibioreactors for the production of monoclonal antibodies in CHO cells." New Biotechnology 33 (July 2016): S112. http://dx.doi.org/10.1016/j.nbt.2016.06.1115.

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13

Pantyushenko, M. S., R. V. Tikhonov, A. A. Piskunov, and V. N. Bade. "Optimization of the Cultivation Processof a Producer Clone of the Recombinant Lysosomal Iduronate-2-sulfatase Enzyme." Biotekhnologiya 37, no. 2 (2021): 34–47. http://dx.doi.org/10.21519/0234-2758-2021-37-2-34-47.

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This paper describes experiments aimed at developing conditions for suspension culturing of a producer clone of the recombinant lysosomal enzyme iduronate-2-sulfatase based on the CHO cell line. As a result of sequential culturing in batch and fed-batch modes, the optimal nutrient medium composition was established, providing a 2.5-fold increase in the iduronate-2-sulfatase specific activity. Optimization of the feed formulation was carried out, which increased the yield of recombinant iduronate-2-sulfatase. The ambr® 15 Cell Culture System of minibioreactors was used to optimize the cultivation process. Chinese hamster ovary cells, CHO, iduronate-2-sulfatase, idursulfase, lysosomal enzyme, sulfatases, Аmbr Tap Biosystems, ambr® 15 Cell Culture System, mucopolysaccharidosis type II, formyl glycine generating enzyme, optimization of cell culturing conditions, fed-batch culture, batch culture.
14

Hernández García, Armando, Eulogio Pimentel Vázquez, and Jesús Mena Campos. "Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide." Electronic Journal of Biotechnology 6, no. 3 (December 15, 2003). http://dx.doi.org/10.2225/vol6-issue3-fulltext-7.

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15

Auchtung, Jennifer M., Catherine D. Robinson, and Robert A. Britton. "Cultivation of stable, reproducible microbial communities from different fecal donors using minibioreactor arrays (MBRAs)." Microbiome 3, no. 1 (September 30, 2015). http://dx.doi.org/10.1186/s40168-015-0106-5.

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16

Bello-Gil, Daniel, Beatriz Maestro, Jennifer Fonseca, Nina Dinjaski, M. Auxiliadora Prieto, and Jesús M. Sanz. "Poly-3-Hydroxybutyrate Functionalization with BioF-Tagged Recombinant Proteins." Applied and Environmental Microbiology 84, no. 4 (December 1, 2017). http://dx.doi.org/10.1128/aem.02595-17.

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ABSTRACTPolyhydroxyalkanoates (PHAs) are biodegradable polyesters that accumulate in the cytoplasm of certain bacteria. One promising biotechnological application utilizes these biopolymers as supports for protein immobilization. Here, the PHA-binding domain of thePseudomonas putidaKT2440 PhaF phasin (BioF polypeptide) was investigated as an affinity tag for thein vitrofunctionalization of poly-3-hydroxybutyrate (PHB) particles with recombinant proteins, namely, full-length PhaF and two fusion proteins tagged to BioF (BioF–C-LytA and BioF–β-galactosidase, containing the choline-binding module C-LytA and the β-galactosidase enzyme, respectively). The protein-biopolyester interaction was strong and stable at a wide range of pHs and temperatures, and the bound protein was highly protected from self-degradation, while the binding strength could be modulated by coating with amphiphilic compounds. Finally, BioF–β-galactosidase displayed very stable enzymatic activity after several continuous activity-plus-washing cycles when immobilized in a minibioreactor. Our results demonstrate the potentialities of PHA and the BioF tag for the construction of novel bioactive materials.IMPORTANCEOur results confirm the biotechnological potential of the BioF affinity tag as a versatile tool for functionalizing PHA supports with recombinant proteins, leading to novel bioactive materials. The wide substrate range of the BioF tag presumably enables protein immobilizationin vitroof virtually all natural PHAs as well as blends, copolymers, or artificial chemically modified derivatives with novel physicochemical properties. Moreover, the strength of protein adsorption may be easily modulated by varying the coating of the support, providing new perspectives for the engineering of bioactive materials that require a tight control of protein loading.
17

Naimi, Sabrine, Emilie Viennois, Andrew T. Gewirtz, and Benoit Chassaing. "Direct impact of commonly used dietary emulsifiers on human gut microbiota." Microbiome 9, no. 1 (March 22, 2021). http://dx.doi.org/10.1186/s40168-020-00996-6.

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Abstract Background Epidemiologic evidence and animal studies implicate dietary emulsifiers in contributing to the increased prevalence of diseases associated with intestinal inflammation, including inflammatory bowel diseases and metabolic syndrome. Two synthetic emulsifiers in particular, carboxymethylcellulose and polysorbate 80, profoundly impact intestinal microbiota in a manner that promotes gut inflammation and associated disease states. In contrast, the extent to which other food additives with emulsifying properties might impact intestinal microbiota composition and function is not yet known. Methods To help fill this knowledge gap, we examined here the extent to which a human microbiota, maintained ex vivo in the MiniBioReactor Array model, was impacted by 20 different commonly used dietary emulsifiers. Microbiota density, composition, gene expression, and pro-inflammatory potential (bioactive lipopolysaccharide and flagellin) were measured daily. Results In accordance with previous studies, both carboxymethylcellulose and polysorbate 80 induced a lasting seemingly detrimental impact on microbiota composition and function. While many of the other 18 additives tested had impacts of similar extent, some, such as lecithin, did not significantly impact microbiota in this model. Particularly stark detrimental impacts were observed in response to various carrageenans and gums, which altered microbiota density, composition, and expression of pro-inflammatory molecules. Conclusions These results indicate that numerous, but not all, commonly used emulsifiers can directly alter gut microbiota in a manner expected to promote intestinal inflammation. Moreover, these data suggest that clinical trials are needed to reduce the usage of the most detrimental compounds in favor of the use of emulsifying agents with no or low impact on the microbiota.
18

Gretty K. Villena. "Assessment of Aspergillus niger biofilm growth kinetics in minibioreactors by carbon dioxide evolution." African Journal of Biotechnology 10, no. 62 (October 12, 2011). http://dx.doi.org/10.5897/ajb10.2672.

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