Добірка наукової літератури з теми "MiR-202"
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Статті в журналах з теми "MiR-202":
1
Lin, Yilin, Zhihua Chen, Suyong Lin, Yan Zheng, Yisu Liu, Ji Gao, and Shaoqin Chen. "MiR-202 inhibits the proliferation and invasion of colorectal cancer by targeting UHRF1." Acta Biochimica et Biophysica Sinica 51, no. 6 (May 6, 2019): 598–606. http://dx.doi.org/10.1093/abbs/gmz042.
Анотація:
Abstract The purpose of this study was to investigate the expression of microRNA-202 (miR-202) and its role in colorectal cancer (CRC) in vivo and in vitro. We examined the expression of miR-202 in CRC tissues by quantitative real-time PCR (qRT-PCR) assay. Lentiviral vectors were constructed to overexpress or inhibit the expression of miR-202 in the CRC cell lines HCT116 and SW480 to determine its effects on cell invasion and proliferation. We found that overexpression of miR-202 significantly inhibited the proliferation and invasion of HCT116 cells. MiRNA target gene prediction, dual luciferase assay, and western blot analysis demonstrated that miR-202 regulated ubiquitin-like with PHD and RING finger domain 1 (UHRF1) expression in both cell lines. The effect of miR-202 on cell proliferation and invasion was partially reversed by activating the expression of UHRF1. Furthermore, miR-202 induced tumor formation in HCT116 xenograft BALB/c nude mice. Mice vaccinated with miR-202-overexpressing cells had smaller tumors and lower UHRF1 expression than the control group. These results indicate the possibility that miR-202 is under-expressed in CRC tissues, and that miR-202 inhibits the proliferation and invasion of CRC via targeting UHRF1. MiR-202 is a potential therapeutic target for CRC.
2
Zhang, Liqing, Jianjiang Xu, Gaodi Yang, Heng Li, and Xiuxia Guo. "miR-202 Inhibits Cell Proliferation, Migration, and Invasion by Targeting Epidermal Growth Factor Receptor in Human Bladder Cancer." Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics 26, no. 6 (July 5, 2018): 949–57. http://dx.doi.org/10.3727/096504018x15149787144385.
Анотація:
Recent studies have demonstrated that miR-202 is associated with several types of cancer; however, the expression and function of miR-202 have not been investigated in bladder cancer. We analyzed the expression of miR-202 in bladder cancer tissues and adjacent noncancerous tissues. The effect of miR-202 on the proliferation, migration, and invasion was evaluated by in vitro assays. The target gene of miR-202 was assessed by luciferase reporter assay. In this study, miR-202 was found to be significantly downregulated in bladder cancer cell lines and tissues and was highly correlated with the T classification, N classification, grade, and recurrence. Ectopic expression of miR-202 suppressed cell viability, colony formation, cell migration, and invasion in vitro and inhibited xenograft tumor growth in vivo. Inversely, downregulation of miR-202 had contradictory effects. The 3′-untranslated region (3′-UTR) of epidermal growth factor receptor (EGFR) was identified as a direct target of miR-202 using luciferase reporter assays, and knockdown of EGFR enhanced miR-202-inhibited cell proliferation, migration, and invasion. In conclusion, miR-202 suppresses bladder cancer carcinogenesis and progression by targeting EGFR, thereby representing a potential target for miRNA-based therapy for bladder cancer in the future.
3
Deng, Xinchao, Congzhe Hou, Zhen Liang, Huali Wang, Lin Zhu, and Hui Xu. "miR-202 Suppresses Cell Proliferation by Targeting FOXR2 in Endometrial Adenocarcinoma." Disease Markers 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/2827435.
Анотація:
Background. MicroRNA-202 (miR-202) has been reported to be aberrantly regulated in several cancers. The aim of this study is to explore the functional role of miR-202 in EAC tumor growth. Material and Methods. miR-202 expression was detected by qRT-PCR. TargetScan and luciferase reporter assay were used to elucidate the candidate target gene of miR-202. The FOXR2 protein level was assessed by Western blot and immunohistochemistry. Survival analysis was explored for FOXR2 expression in EAC patients. Results. miR-202 expression was significantly decreased in EAC tissues (P<0.01) compared with that in control tissues. And the downregulate miR-202 was significantly associated with poor prognosis (P<0.01). Re-expression of miR-202 dramatically suppressed cell proliferation in vitro and tumor growth in vivo. FOXR2 was identified as a direct target of miR-202. In EAC tissues, FOXR2 was upregulated and the increased FOXR2 was significantly associated with poor prognosis. In miR-202-transfected cells, the FOXR2 expression was inversely changed. The analysis of FOXR2 protein expression and miR-202 transcription in EAC tissues showed negative correlation (R=−0.429). Conclusion. miR-202 may function as a tumor suppressor in EAC tumor growth by targeting FOXR2 oncogene, which may provide new insights into the molecular mechanism and new targets for treatment of EAC.
4
Zhuang, Donghai, Li Liang, Hongzhan Zhang, and Xianguang Feng. "miR-202 Suppresses Hepatocellular Carcinoma Progression via Downregulating BCL2 Expression." Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics 28, no. 4 (September 1, 2020): 399–408. http://dx.doi.org/10.3727/096504020x15864296270581.
Анотація:
miRNAs play an important role in progression of hepatocellular carcinoma (HCC). In this work, we assessed the function of miR-202 in human HCC and identified BCL2 as its target. We found miR-202 expression was found significantly downregulated, while BCL2 expression was markedly upregulated in HCC tissues and cell lines (HepG2, Hep3B, and HCCLM3). Both miR-202 and BCL2 were closely correlated with major vascular invasion and advanced TNM stage as well as overall survival of HCC patients. Overexpression of miR-202 significantly inhibited cell proliferation, induced apoptosis and cell cycle arrest at the G0/G1 phase, and prevented tumor formation in a xenograft nude mouse model. Further, miR-202 dramatically inhibited migration, invasion, and epithelialmesenchymal transition. miR-202 bound to the 3-untranslated region (3-UTR) of BCL2 mRNA and downregulated the expression level of BCL2 protein. Exogenous BCL2 overexpression weakened the inhibitory effects of miR-202, while inhibition of BCL2 enhanced the inhibitory effects of miR-202. In conclusion, miR-202 serves as a tumor suppressor in HCC progression by downregulating BCL2 expression, indicating miR-202 might be a potential target for HCC.
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WANG, Qifeng, and Xiang Du. "Effect of microRNA-202-3p on cell proliferation and targeting of ADP-ribosylation factor-like 5A in human colorectal carcinoma." Journal of Clinical Oncology 31, no. 15_suppl (May 20, 2013): e14667-e14667. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.e14667.
Анотація:
e14667 Background: MicroRNAs (miRNAs) are small, non-coding RNAs that are strongly implicated in cancer and reshaped our understanding of the role of non-protein-coding genes in carcinogenesis. Methods: Quantitative RT-PCR was used to evaluate miR-202-3p expression in 94 pairs of human primary CRC and adjacent noncancerous tissues (NCT) to determine the clinicopathologic significance of miR-202-3p. Cell proliferation analysis and Xenograft analysis was used to investigate miR-202-3p function in vitro and in vivo.It’s direct target ARL5A was confirmed by Luciferase assay and Western blot. Immunochemistry was performed to reveal endogenous protein level of ARL5A and analysis clinical significance. Results: In this study, miR-202-3p was verified significantly down regulated in 46.7% (44/94) of the CRC tissues when compared to the corresponding noncancerous tissues(NCT). Subsequently, DNA copy number deletion of Pre-miR-202 in 63.2%(24/38) CRC tissue was proved(compared with NCT), which was considered as a main cause of the low expression of miR-202-3p. Cell proliferation analysis and colony formation assay showed that overexpression of miR-202-3p inhibit CRC cell growth in vitro. Xenograft analysis revealed that ectopic expression of miR-202-3p decreased the tumorigenicity of CRC cells in vivo. Consistent with these results, silencing of miR-202-3p resulted in a increased growth ratio of the colon cancer cells. In addition, ADP-ribosylation factor-like 5A(ARL5A) was predicted as potential target of miR-202-3p which was confirmed by Luciferase assay and Western blot. Overexpression of miR-202-3p could reduced the endogenous protein level of ARL5A, whereas silencing of miR-202-3p obviously up regulated ARL5A expression. In human CRC tissues, miR-202-3p expression levels correlated inversely with ARL5A protein levels which was identified as an prognostic factor in this study. Furthermore, knockdown of ARL5A phenocopied the proliferation-inhibiting effect of miR-202-3p. Conclusions: These results indicated that miR-202-3p, acting as a new tumor suppressor in CRC, could decrease cell proliferation via directly targeting ARL5A, a first reported gene related to CRC prognosis.
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Ding, Qiang, Miaohan Jin, Yaoyue Wang, Jiao Liu, Peter Kalds, Ying Wang, Yuxin Yang, Xiaolong Wang та Yulin Chen. "Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2". Cells 9, № 2 (14 лютого 2020): 445. http://dx.doi.org/10.3390/cells9020445.
Анотація:
MicroRNAs play key roles during ovary development, with emerging evidence suggesting that miR-202-5p is specifically expressed in female animal gonads. Granulosa cells (GCs) are somatic cells that are closely related to the development of female gametes in mammalian ovaries. However, the biological roles of miR-202-5p in GCs remain unknown. Here, we show that miR-202-5p is specifically expressed in GCs and accumulates in extracellular vesicles (EVs) from large growth follicles in goat ovaries. In vitro assays showed that miR-202-5p induced apoptosis and suppressed the proliferation of goat GCs. We further revealed that miR-202-5p is a functional miRNA that targets the transforming growth factor-beta type II receptor (TGFβR2). MiR-202-5p attenuated TGF-β/SMAD signaling through the degradation of TGFβR2 at both the mRNA and protein level, decreasing p-SMAD3 levels in GCs. Moreover, we verified that steroidogenic factor 1 (SF1) is a transcriptional factor that binds to the promoters of miR-202 and cytochrome P450 family 19 subfamily A member 1 (CYP19A1) through luciferase reporter and chromatin immunoprecipitation (ChIP) assays. That contributed to positive correlation between miR-202-5p and CYP19A1 expression and estradiol (E2) release. Furthermore, SF1 repressed TGFβR2 and p-SMAD3 levels in GCs through the transactivation of miR-202-5p. Taken together, these results suggest a mechanism by which miR-202-5p regulates canonical TGF-β/SMAD signaling through targeting TGFβR2 in GCs. This provides insight into the transcriptional regulation of miR-202 and CYP19A1 during goat ovarian follicular development.
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Ahmed, Emad A., Peramaiyan Rajendran, and Harry Scherthan. "The microRNA-202 as a Diagnostic Biomarker and a Potential Tumor Suppressor." International Journal of Molecular Sciences 23, no. 11 (May 24, 2022): 5870. http://dx.doi.org/10.3390/ijms23115870.
Анотація:
MicroRNA-202 (miR-202) is a member of the highly conserved let-7 family that was discovered in Caenorhabditis elegans and recently reported to be involved in cell differentiation and tumor biology. In humans, miR-202 was initially identified in the testis where it was suggested to play a role in spermatogenesis. Subsequent research showed that miR-202 is one of the micro-RNAs that are dysregulated in different types of cancer. During the last decade, a large number of investigations has fortified a role for miR-202 in cancer. However, its functions can be double-edged, depending on context they may be tumor suppressive or oncogenic. In this review, we highlight miR-202 as a potential diagnostic biomarker and as a suppressor of tumorigenesis and metastasis in several types of tumors. We link miR-202 expression levels in tumor types to its involved upstream and downstream signaling molecules and highlight its potential roles in carcinogenesis. Three well-known upstream long non-coding-RNAs (lncRNAs); MALAT1, NORAD, and NEAT1 target miR-202 and inhibit its tumor suppressive function thus fueling cancer progression. Studies on the downstream targets of miR-202 revealed PTEN, AKT, and various oncogenes such as metadherin (MTDH), MYCN, Forkhead box protein R2 (FOXR2) and Kirsten rat sarcoma virus (KRAS). Interestingly, an upregulated level of miR-202 was shown by most of the studies that estimated its expression level in blood or serum of cancer patients, especially in breast cancer. Reduced expression levels of miR-202 in tumor tissues were found to be associated with progression of different types of cancer. It seems likely that miR-202 is embedded in a complex regulatory network related to the nature and the sensitivity of the tumor type and therapeutic (pre)treatments. Its variable roles in tumorigenesis are mediated in part thought its oncogene effectors. However, the currently available data suggest that the involved signaling pathways determine the anti- or pro-tumorigenic outcomes of miR-202’s dysregulation and its value as a diagnostic biomarker.
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Kim, Jungho, Sunyoung Park, Dasom Hwang, Seung Il Kim, and Hyeyoung Lee. "Diagnostic Value of Circulating miR-202 in Early-Stage Breast Cancer in South Korea." Medicina 56, no. 7 (July 9, 2020): 340. http://dx.doi.org/10.3390/medicina56070340.
Анотація:
Background and objectives: Breast cancer is the most common cancer among women worldwide. Early stage diagnosis is important for predicting increases in treatment success rates and decreases in patient mortality. Recently, circulating biomarkers such as circulating tumor cells, circulating tumor DNA, exosomes, and circulating microRNAs have been examined as blood-based markers for the diagnosis of breast cancer. Although miR-202 has been studied for its function or expression in breast cancer, its potential diagnostic value in a clinical setting remains elusive and miR-202 has not been investigated in South Korea. In this study, we aimed to evaluate the diagnostic utility of miR-202 in plasma samples of breast cancer patients in South Korea. Materials and Methods: We investigated miR-202 expression in the plasma of 30 breast cancer patients during diagnosis along with 30 healthy controls in South Korea by quantitative reverse transcription PCR. Results: The results showed that circulating miR-202 levels were significantly elevated in the breast cancer patients compared with those in healthy controls (p < 0.001). The sensitivity and specificity of circulating miR-202 were 90.0% and 93.0%, respectively. Additionally, circulating miR-202 showed high positivity at early stage. The positive rate of miR-202 was as follows: 100% (10/10) for stage I, 90% (9/10) for stage II, and 80% (8/10) for stage III. miR-202 was also a predictor of a 9.6-fold high risk for breast cancer (p < 0.001). Conclusions: Additional alternative molecular biomarkers for diagnosis and management of pre-cancer patients are needed. Circulating miR-202 might be potential diagnostic tool for detecting early stage breast cancer.
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Mo, Chengyu, Wenjing Li, Kuntong Jia, Wei Liu, and Meisheng Yi. "Proper Balance of Small GTPase rab10 Is Critical for PGC Migration in Zebrafish." International Journal of Molecular Sciences 22, no. 21 (November 4, 2021): 11962. http://dx.doi.org/10.3390/ijms222111962.
Анотація:
MicroRNAs (miRNAs) play important roles in post-transcriptional repression in nearly every biological process including germ cell development. Previously, we have identified a zebrafish germ plasm-specific miRNA miR-202-5p, which regulates PGC migration through targeting cdc42se1 to protect cdc42 expression. However, knockdown of cdc42se1 could not significantly rescue PGC migration in maternal miR-202 mutant (MmiR-202) embryos, indicating that there are other target genes of miR-202-5p required for the regulation of PGC migration. Herein, we revealed the transcriptional profiles of wild type and MmiR-202 PGCs and obtained 129 differentially expressed genes (DEGs), of which 42 DEGs were enriched cell migration-related signaling pathways. From these DEGs, we identified two novel miR-202-5p target genes prdm12b and rab10. Furthermore, we found that disruption of rab10 expression led to significantly migratory defects of PGC by overexpression of rab10 siRNA, or WT, inactive as well as active forms of rab10 mRNA, and WT rab10 overexpression mediated migratory defects could be partially but significantly rescued by overexpression of miR-202-5p, demonstrating that rab10 is an important factor involved miR-202-5p mediated regulation of PGC migration. Taken together, the present results provide significant information for understanding the molecular mechanism by which miR-202-5p regulates PGC migration in zebrafish.
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Sun, Wei, Wei Ping, Yitao Tian, Wenbin Zou, Jiawei Liu, and Yukun Zu. "miR-202 Enhances the Anti-Tumor Effect of Cisplatin on Non-Small Cell Lung Cancer by Targeting the Ras/MAPK Pathway." Cellular Physiology and Biochemistry 51, no. 5 (2018): 2160–71. http://dx.doi.org/10.1159/000495835.
Анотація:
Background/Aims: KRas is usually mutated in non-small cell lung cancer (NSCLC). The mutated KRas gene is a negative prognostic indicator that promotes tumor proliferation, metastasis, and drug resistance in NSCLC, and thus has become a target for cancer therapy. This study is focused on the effects of the microRNA (miR)-202/KRas axis in regulating chemosensitivity in NSCLC. Methods: Quantitative reverse transcriptase real-time PCR analysis was performed to examine the expression of miR-202. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays were performed to evaluate the sensitivity of cisplatin against NSCLC cells. The miR-202/KRas axis was confirmed by western blot and luciferase reporter assays. Cell apoptosis was measured by flow cytometry. KRas expression, MEK1/2 and ERK1/2 phosphorylation, and activation of caspase-9 and caspase-3 were detected by western blot. Results: A significant decrease in miR-202 expression was observed in NSCLC cells both in vivo and in vitro. In addition, miR-202 expression was associated with drug resistance. Recovery of miR-202 expression levels was found to increase the sensitivity of both NCI-H441 and A549 NSCLC cells to cisplatin treatment. Mechanically, as the Ras/mitogen-activated protein kinase (MAPK) pathway was aberrantly activated in NCI-H441 and A549 NSCLC cells, the overexpression of miR-202 was found to inhibit the Ras/MAPK pathway by targeting the KRas gene. As a result, increased miR-202 expression expanded apoptosis signaling induced by cisplatin in NSCLC cells. Conclusion: The miR-202/KRas axis controlled the chemosensitivity of NSCLC by mediating the Ras/MAPK pathway. Thus, the combination of platinum-based drugs with miR-202 may represent a novel strategy to enhance the anti-tumor effect against NSCLC.
Дисертації з теми "MiR-202":
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Lesage, Manon. "Exploration tridimensionnelle des aspects dynamiques de l’ovogenèse et de sa régulation par le miR-202 chez le médaka Oryzias latipes." Electronic Thesis or Diss., Université de Rennes (2023-....), 2023. https://ged.univ-rennes1.fr/nuxeo/site/esupversions/d7fe9d24-92e2-403f-9db1-f514a508e140.
Анотація:
L'ovaire est caractérisé par des processus de croissance, de stockage et de recrutement des ovocytes entraînant d'importants réarrangements cellulaires au cours du cycle de vie de la femelle. Chez le médaka Oryzias latipes, comme chez d'autres poissons téléostéens, ces mécanismes d'ovogenèse sont mal compris d'un point de vue quantitatif et restent souvent extrapolés à partir de fractions de l'organe entier. Par conséquent, la connaissance de la dynamique des populations d'ovocytes dans l'ovaire est limitée, alors qu'elle est essentielle pour comprendre la régulation de la fertilité, notamment chez les espèces à intervalles de ponte courts comme le médaka. Pour y remédier, nous avons développé une méthode d'analyse des ovaires entiers qui est exhaustive, accessible et également transférable à d'autres espèces. En combinant la transparisation des tissus, la microscopie confocale 3D et l'analyse d'images assistée par des algorithmes d'apprentissage profond, notre méthode a permis d'extraire des données quantitatives et morphologiques à différents stades de développement. L'analyse de l'évolution du contenu ovarien au cours du temps a révélé l'établissement de différentes réserves folliculaires au cours de la croissance/maturation de la femelle, indiquant la présence de deux recrutements majeurs qui alimentent progressivement une distribution folliculaire asynchrone chez l'adulte reproducteur. L'étude de la lignée miR-202-KO, dont la fertilité est réduite, a mis en évidence l'importance du maintien de la distribution asynchrone des follicules pour l'établissement d'un taux de fertilité normal. Les dérégulations des profils de distribution des follicules observées chez les femelles miR-202-KO sont cohérentes avec le rôle de miR- 202 dans la régulation du recrutement des follicules au milieu et à la fin de la croissance primaire. Grâce au développement de méthodes innovantes, cette étude a ainsi permis de mieux comprendre la dynamique ovarienne chez les poissons, ainsi que la manière dont miR-202 est impliqué dans sa régulationThe ovary is characterized by processes of oocyte growth, storage and recruitment, thus resulting in important cellular rearrangements during the female life cycle. In medaka Oryzias latipes, as in other teleost fish, these mechanisms of oogenesis are poorly understood from a quantitative point of view and often remain extrapolated from fractions of the whole organ. As a consequence, knowledge of oocyte population dynamics in the ovary is limited, although this is essential for understanding the regulation of fertility, most notably in species with short spawning intervals such as medaka. To address this, we have developed a method for analyzing whole ovaries that is comprehensive, accessible and also transferable to other species. By combining tissue clearing, 3D confocal microscopy and image analysis assisted by deep learning algorithms, our method allowed the extraction of quantitative and morphological data at different stages of development. The analysis of ovarian content changes over time revealed the establishment of different follicular reserves during female growth/maturation, indicating the presence of two major recruitments that progressively supply asynchronous follicular distribution in the reproducing adult. The study of the miR-202-KO line with reduced fertility has highlighted the importance of maintaining the asynchronous distribution of follicles for the establishment of a normal fertility rate. The deregulations of follicle distribution patterns observed in miR-202-KO females were consistent with a role for miR-202 in regulating follicle recruitment during mid and late primary growth. Through the development of innovative methods, this study provided a new understanding of ovarian dynamics in fish, as well as a better comprehension of how miR-202 is involved in its regulation
Частини книг з теми "MiR-202":
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"Vorwort." In Das Geschlecht in mir, edited by Gerhard Schreiber, XI—XX. Berlin, Boston: De Gruyter, 2019. http://dx.doi.org/10.1515/9783110614626-202.
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D’Angelo, Marina. "4 Ricordare è rivivere. Lavarone, Florenz, Rom 1907." In »So will ich mir entfliehen«, 202–20. Psychosozial-Verlag, 2020. http://dx.doi.org/10.30820/9783837977059-202.
3
Puchta, Jonas. "II.5 Arten der Demut." In „Du bist mir noch nicht demüthig genug“, 202–306. Verlag Karl Alber, 2021. http://dx.doi.org/10.5771/9783495825570-202.
Тези доповідей конференцій з теми "MiR-202":
1
Hoffman, Aaron E., Ran Liu, Tongzhang Zheng, Alan Fu, Frank Slack, and Yong Zhu. "Abstract 1652: Targetome profiling and genetic association analyses implicate miR-202 in follicular lymphomagenesis." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-1652.