Готові списки джерел за темами / Molecular cloning

Зміст

  1. Статті в журналах
  2. Дисертації
  3. Книги
  4. Частини книг
  5. Тези доповідей конференцій
  6. Звіти організацій

Добірка наукової літератури з теми "Molecular cloning"

Автор: Grafiati
Опубліковано: 4 червня 2021
Оновлено: 25 липня 2025

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Статті в журналах з теми "Molecular cloning"

1

Xu, Libing, Yuhong Chen, Qiuhua Li, Tianliang He, and Xinhua Chen. "Molecular cloning." Fish & Shellfish Immunology 98 (March 2020): 981–87. http://dx.doi.org/10.1016/j.fsi.2019.10.064.

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2

Kwak, Inseok. "Molecular Cloning and Characterization of Bovine CYP26A1 Promoter." Journal of Life Science 26, no. 1 (2016): 42–49. http://dx.doi.org/10.5352/jls.2016.26.1.42.

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3

Tu, Kevin, Angela Sun, and Daniel Levin. "A Sweet Method of Modeling Restriction Endonuclease-Based Molecular Cloning." American Biology Teacher 85, no. 1 (2023): 52–54. http://dx.doi.org/10.1525/abt.2023.85.1.52.

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Анотація:
Molecular cloning is an invaluable research tool in modern molecular biology. However, it is often difficult for students to grasp conceptually without visual aids and even more difficult to understand how to successfully set up a cloning experiment. Here, we describe a flipped classroom activity that simulates cloning using donuts as models of plasmids. Students noted in semistructured interviews that the interactive nature of this activity made it an engaging introduction to molecular cloning.
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4

Morimura, Naoko, Yoko Tezuka, Naoko Watanabe, et al. "Molecular Cloning of POEM." Journal of Biological Chemistry 276, no. 45 (2001): 42172–81. http://dx.doi.org/10.1074/jbc.m103216200.

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5

Ashwini, Malla, Shanmugaraj Bala Murugan, Srinivasan Balamurugan, and Ramalingam Sathishkumar. "Advances in molecular cloning." Molecular Biology 50, no. 1 (2016): 1–6. http://dx.doi.org/10.1134/s0026893316010131.

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6

Taylor, D. W., J. S. Cordingley, D. W. Dunne, et al. "Molecular cloning of schistosome genes." Parasitology 92, S1 (1986): S73—S81. http://dx.doi.org/10.1017/s003118200008570x.

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As part of an integrated programme investigating human schistosomiasis, work which involves epidemiological surveys and detailed immunological studies as well as biochemical investigations, we have, over the last three years, been cloning schistosome genes in a variety of plasmid and lambda vector systems. In this lecture we present a review of some selected aspects of work primarily aimed at production of experimental vaccines against the disease but which, on a broader front, is also concerned with developmental regulation of gene expression around the parasite's life-cycle. Specifically, we
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7

Yamamoto, Kosuke, Suguru Oguri, Susumu Chiba, and Yoshie S. Momonoki. "Molecular cloning ofacetylcholinesterasegene fromSalicornia europaeaL." Plant Signaling & Behavior 4, no. 5 (2009): 361–66. http://dx.doi.org/10.4161/psb.4.5.8360.

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8

Iwaki, Daisuke, Shun-ichiro Kawabata, Yoshiki Miura, et al. "Molecular Cloning of Limulusalpha2-Macroglobulin." European Journal of Biochemistry 242, no. 3 (1996): 822–31. http://dx.doi.org/10.1111/j.1432-1033.1996.0822r.x.

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9

Malcolm, S. "Guide to Molecular Cloning Techniques." Journal of Medical Genetics 27, no. 1 (1990): 70. http://dx.doi.org/10.1136/jmg.27.1.70.

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10

Reuter, Harald, and Hartmut Porzig. "Muscle disease and molecular cloning." Nature 336, no. 6195 (1988): 113. http://dx.doi.org/10.1038/336113b0.

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Дисертації з теми "Molecular cloning"

1

Bates, Nancy Carol. "Characterization of cbg : a cloned gene encoding an extracellular [beta]-glucosidase from Cellulomonas fimi." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26163.

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A group of Escherichia coli clones harbouring recombinant pBR322 plasmid, containing Cellulomonas fimi DNA inserts, that reacted with antiserum to C.fimi culture supernatant, was screened for their ability to hydrolyze carboxymethyl cellulose (CMC) and 4-methylumbeliferyll-β-D-cellobioside (MUC). A clone, pEC62, hydrolyzed MUC but did not hydrolyze CMC. The recombinant enzyme encoded by pEC62 was shown to be a β-glucosidase (cellobiase). C.fimii itself was shown to encode an extracellular β-glucosidase in C.fimi. This is the first report of an extracellular β-glucosidase from a bacterium. Del
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2

Seto, Nina Oi Ling. "The copper-zinc superoxide dismutase gene from Drosophila melanogaster : attempts to clone the gene using two mixed sequence oligonucleotide probes." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26534.

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Superoxide dismutase is an enzyme which scavenges superoxide radicals and is thought to be a longevity determinant, as there exists a positive correlation between superoxide dismutase concentration and maximum life span potential. The cytosolic CuZn superoxide dismutase in D. melanogaster has been purified and sequenced, but the gene has not been cloned. However, when it is available the CuZn SOD gene may be reintroduced into the Drosophila genome via the P-element transformation system so its effects on the life span potential of Drosophila may be studied. This study describes attempts to clo
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3

Woodruff, Wendy Anne. "Cloning and characterization of the oprF gene for protein F from Pseudomonas aeruginosa." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29218.

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The oprF gene encoding porin protein F from Pseudomonas aeruginosa was cloned onto a cosmid vector into Escherichia coli. Protein F was expressed in large amounts in E. coli and retained its heat- and reduction-modifiable and immunological characteristics. The cloned oprF gene product was purified from E. coli and characterized with respect to pore-forming ability in black lipid bilayers. Small channels, with an average single channel conductance of approximately 0.4 nS, were observed. A similar small channel size was observed for native protein F. The oprF sequences were used as a DNA-DNA hyb
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4

Boyes, Barry Edward. "Molecular cloning of the human Substantia innominata : characterization of a brain large mRNA." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/30960.

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Brain tissue samples were collected from individuals with histologically and biochemically confirmed Alzheimer's Disease (AD), as well as from a group of individuals without any signs of neurological disease (NNC). Ribonucleic acid (RNA) was extracted from these tissues, characterized by several chemical methods, and the yields were compared between AD and NNC groups. High molecular weight RNA could be effectively extracted from frozen postmortem human brain. In comparison to the NNC group, tissue RNA levels were reduced in the AD hippocampus, but not in the temporal cortex or substantia innom
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5

Lush, Michael John. "Molecular cloning of neuropathy target esterase." Thesis, University of Leicester, 1998. http://hdl.handle.net/2381/29627.

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Анотація:
A single ingestion of certain organophosphorus esters (OPs) can cause a syndrome known as Organophosphate Induced Delayed Polyneuropathy (OPIDP), a paralysing neuropathy with degeneration of long axons, developing after a latent period of approximately one to three weeks. The primary target of these OPs has been shown to be a 155kDa neural protein designated Neuropathy Target Esterase (NTE), and the toxic effects apparently due to the covalent inhibition and subsequent secondary modification of this protein. Recently NTE has been purified to apparent homogeneity using a novel biotinylated OP a
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6

Long, Graham Stanley. "Molecular cloning of bacteriophage K1E endosialidase." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339539.

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7

McNair, Alan Thomas. "Molecular cloning of Fasciola hepatica antigens." Thesis, Queen's University Belfast, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335604.

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8

Choi, Wai To. "Molecular cloning of ribosome-inactivating proteins." HKBU Institutional Repository, 1996. http://repository.hkbu.edu.hk/etd_ra/63.

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9

Fisher, Adam B. "ex vivo DNA cloning." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3962.

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Анотація:
Genetic engineering of microbes has developed rapidly along with our ability to synthesize DNA de novo. Yet, even with decreasing DNA synthesis costs there remains a need for inexpensive, rapid and reliable methods for assembling synthetic DNA into larger constructs or combinatorial libraries. While technological advances have resulted in powerful techniques for in vitro and in vivo assembly of DNA, each suffers inherent disadvantages. Here, an ex vivo DNA cloning suite using crude cellular lysates derived from E. coli is demonstrated to amplify and assemble DNA containing small sequence homol
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10

Wakarchuk, Warren William. "The molecular cloning and characterization of a Beta-glucosidase gene from an Agrobacterium." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/27559.

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The β-glucosidase (Abg) from ATCC 21400, an Agrobacterium species, was purified to homogeneity. The protein was cleaved with cyanogen bromide and the peptides were purified by reversed phase high pressure liquid chromatography. The partial amino-acid sequences for three CNBr peptides, CNBr1, CNBr2 and CNBr3, were determined by automated Edman degradation. A sequence from CNBr2 was used to synthesize a mixture of oligonucleotides which was used as a hybridization probe to identify a recombinant DNA clone carrying the gene for β-glucosidase. A single clone was isolated which expressed an enzymat
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Книги з теми "Molecular cloning"

1

A, Lund Peter, and Minchin Steve, eds. Gene cloning. Taylor & Francis Group, 2007.

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2

Habener, Joel F., ed. Molecular Cloning of Hormone Genes. Humana Press, 1987. http://dx.doi.org/10.1007/978-1-4612-4824-8.

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3

Sambrook, Joseph. Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory, 1989.

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4

Sambrook, Joseph. Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory, 1989.

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5

Sambrook, Joseph. Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory, 1989.

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6

Sambrook, Joseph. Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory, 1987.

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7

Sambrook, Joseph. Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory, 1989.

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8

Joseph, Sambrook, and Sambrook Joseph, eds. Molecular cloning: A laboratory manual. 4th ed. Cold Spring Harbor Laboratory Press, 2012.

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9

F, Fritsch E., and Maniatis Tom, eds. Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory, 1989.

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10

F, Habener Joel, ed. Molecular cloning of hormone genes. Humana Press, 1987.

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Частини книг з теми "Molecular cloning"

1

Brown, T. A. "Cloning genes." In Genetics: A Molecular Approach. Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2312-9_20.

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2

Deichmann, Annette, and Klaus Deichmann. "Cloning Vectors." In Techniques in Molecular Medicine. Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59811-1_15.

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3

Ohara, Osamu. "ORFeome Cloning." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-232-2_1.

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4

Fontes, Andrew. "Cloning Technologies." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-348-0_20.

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5

Watson, Jake F., Sandra Arroyo-Urea, and Javier García-Nafría. "DNA Cloning." In Handbook of Molecular Biotechnology. CRC Press, 2024. http://dx.doi.org/10.1201/9781003055211-8.

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6

Liu, Dongyou. "RNA Cloning." In Handbook of Molecular Biotechnology. CRC Press, 2024. http://dx.doi.org/10.1201/9781003055211-18.

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7

Gulcher, Jeffrey, and Kari Stefansson. "Positional Cloning." In Methods in Molecular Medicine™. Humana Press, 2006. http://dx.doi.org/10.1007/978-1-59745-159-8_10.

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8

Li, Duanxiang. "Positional Cloning." In Methods in Molecular Medicine™. Humana Press, 2006. http://dx.doi.org/10.1007/978-1-59745-159-8_9.

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9

Julin, Douglas. "Plasmid Cloning Vectors." In Molecular Life Sciences. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4614-6436-5_86-1.

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Julin, Douglas. "Cloning Vector Compatibility." In Molecular Life Sciences. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4614-6436-5_92-4.

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Тези доповідей конференцій з теми "Molecular cloning"

1

Edgington, T. S., J. H. Morrissey, and H. Fakhrai. "MOLECULAR CLONING OF HUMAN TISSUE FACTOR cDNA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643740.

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Tissue factor (TF), the cell-surface receptor and allo-steric activator for factor Vll/VIIa, is important in hemostasis andinflammation. The TF apoprotein was purifiedfrom human brain using factor Vll-affinitychromatography and SDS gel electrophoresis,and was found to consist of a 47 kDa heavy chain plus a 12.5 kDa light chain. Approximately one-third of the heavy chain amino acid sequence was determined for four regions by microsequencing the intact protein and peptides derived from V8-protease digestion. A λgtll cDNA library, made from mRNA derived from the human fibroblastic cell line WI38,
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2

Lopez, J. A., D. W. Chung, K. Fujikawa, F. S. Hagen, T. Papavannopoulou, and G. J. Roth. "MOLECULAR CLONING OF HUMAN PLATELET GLYCOPROTEIN Ib." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642927.

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Glycoprotein Ib (GPIb) mediates von Willebrand factor-dependent platelet adhesion and participates in the resulting platelet activation process. In the present investigation, the primary structure of human platelet GPIb was studied. GPIb and its proteolytic fragment glycocalicin were purified to near homogeneity from human platelets by affinity chromatography using wheat germ agglutinin and anti-GPIb monoclonal antibody (D. Nugent, University of Washington) coupled to Sepharose. GPIba chain, β chain, and glycocalicin were isolated, reduced and carboxymethylated, and then fragmented by trypsin
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3

Fang, GuiJie, and XianFeng Qiao. "Molecular Cloning and Analysis of Hubei White Swine Myostatin Gene." In 2010 2nd International Conference on Information Engineering and Computer Science (ICIECS). IEEE, 2010. http://dx.doi.org/10.1109/iciecs.2010.5678159.

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4

Sun Qingpeng and Yu Yongkun. "Molecular cloning and bioinformatics analysis of genomic DNA of LeWRKY1." In 2010 2nd International Conference on Information Science and Engineering (ICISE). IEEE, 2010. http://dx.doi.org/10.1109/icise.2010.5690845.

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5

Yin, Heng, Xiaoming Zhao, and Yuguang Du. "Cloning and Molecular Characterization of a SKP1 Gene from Brassica napus." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5162512.

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6

Moheer, Reyad Qaed Al, Farah Diba Abu Bakar, and Abdul Munir Abdul Murad. "Molecular cloning and characterization of alpha - galactosidase gene from Glaciozyma antarctica." In THE 2015 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. AIP Publishing LLC, 2015. http://dx.doi.org/10.1063/1.4931247.

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Uzan, G., A. Lajmanovich, M. H. Prandini, Ph Frachet, A. Duperray, and G. Marguerie. "MOLECULAR CLONING OF PLATELET GPIIb FROM HEL CELLS AND HUMAN MEGAKARYOCYTES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643960.

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Platelet GP IIb-IIIa is an heterodimer which functions as a receptor for fibrinogen, fibronectin and Von Willebrand factor and is implicated in platelet adhesive reactions. To study the structure function relationship of this glycoprotein, a recombinant DNA approach was initiated. cDNA expression libraries were constructed in » gtll vector, from erythro-leukemia cells (HEL) and megakaryocytes mRNA. The human megakaryocytes were isolated from patients with chronic myeloid leukemia. The HEL library was initially screened with polyclonal antibodies anti GPIIb IIIa. One clone, λIIbI, containing a
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Zhang, Haiyan, Hongfang Ji, Lingwen Zhang, Xingquan Wu, and Shihua Chen. "Molecular cloning and sequence analysis of the endochitinase from Chaetomium cupreum." In 2010 3rd International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2010. http://dx.doi.org/10.1109/bmei.2010.5639736.

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Qiu, Xingyang, Pei Ge, Yueyue Wang, and Hong Zhou. "Molecular Cloning and Identification of STAT4 in Grass Carp (Ctenopharyngodon idella)." In ICBBT '21: 2021 13th International Conference on Bioinformatics and Biomedical Technology. ACM, 2021. http://dx.doi.org/10.1145/3473258.3473272.

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Zhou, Tao, Anchun Cheng, Mingshu Wang, et al. "Molecular Cloning and Characterization of the UL10 Gene from Duck Enteritis Virus." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5515899.

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Звіти організацій з теми "Molecular cloning"

1

Kun, Ernest. Molecular Cloning of Adenosinediphosphoribosyl Transferase. Defense Technical Information Center, 1987. http://dx.doi.org/10.21236/ada185458.

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2

Drager, Robert. Molecular cloning of spinach chloroplast DNA isolated by alkaline lysis. Portland State University Library, 2000. http://dx.doi.org/10.15760/etd.5631.

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3

Sebastian S. Cocioba, Sebastian S. Cocioba. Toward a Universal, Frugal, and Antibiotic-Free Sugar Selection System for Molecular Cloning. Experiment, 2024. http://dx.doi.org/10.18258/69244.

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Robb, Frank T. Molecular Profiling of Microbial Communities from Contaminated Sources: Use of Subtractive Cloning Methods and rDNA Spacer Sequences. Office of Scientific and Technical Information (OSTI), 2001. http://dx.doi.org/10.2172/781022.

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Robb, Frank T. Molecular Profiling of Microbial Communities from Contaminated Sources: Use of Subtractive Cloning Methods and rDNA Spacer Sequences. Office of Scientific and Technical Information (OSTI), 2000. http://dx.doi.org/10.2172/827425.

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Dellaporta, S. L. Molecular cloning and structural characterization of the R locus maize: Annual progress report, September 1, 1987--May 31, 1988. Office of Scientific and Technical Information (OSTI), 1988. http://dx.doi.org/10.2172/6412467.

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Krawiec, S. Molecular biological enhancement of coal desulfurization: Cloning and expression of the sulfoxide/sulfone/sulfonate/sulfate genes in Pseudomonads and Thiobacillae. Office of Scientific and Technical Information (OSTI), 1991. http://dx.doi.org/10.2172/5587437.

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Krawiec, S. Molecular biological enhancement of coal desulfurization: Cloning and expression of the sulfoxide/sulfone/sulfonate/sulfate genes in Pseudomonads and Thiobacillae. Office of Scientific and Technical Information (OSTI), 1991. http://dx.doi.org/10.2172/6224900.

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9

Zhang, Hongbin B., David J. Bonfil, and Shahal Abbo. Genomics Tools for Legume Agronomic Gene Mapping and Cloning, and Genome Analysis: Chickpea as a Model. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586464.bard.

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The goals of this project were to develop essential genomic tools for modern chickpea genetics and genomics research, map the genes and quantitative traits of importance to chickpea production and generate DNA markers that are well-suited for enhanced chickpea germplasm analysis and breeding. To achieve these research goals, we proposed the following research objectives in this period of the project: 1) Develop an ordered BAC library with an average insert size of 150 - 200 kb (USA); 2) Develop 300 simple sequence repeat (SSR) markers with an aid of the BAC library (USA); 3) Develop SSR marker
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Zamir, Dani, and Steven Tanksley. Fine Mapping and Genetic Interactions of Nearly-Isogenic Allelic Series Representing Yield and Quality QTLs Derived from Wild Tomato Species. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7586460.bard.

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Анотація:
Wild germplasm represents a rich source of QTLs capable of enhancing productivity of crop plants. Using the molecular linkage map of tomato in conjunction with novel population structures, we have identified QTLs from five Lycopersicon species that improve key yield and quality associated traits of processing tomatoes. In this research we employed multi-testing sites for fine mapping analysis of the different components of the affected traits combined with genetic interaction studies. Our results demonstrate that 'exotic libraries', which comprise of marker-defined genomic regions taken from w
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