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Статті в журналах з теми "MRNA report"
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Shen, Qingtang, Yifan E. Wang, Mathew Truong, Kohila Mahadevan, Jingze J. Wu, Hui Zhang, Jiawei Li, Harrison W. Smith, Craig A. Smibert, and Alexander F. Palazzo. "RanBP2/Nup358 enhances miRNA activity by sumoylating Argonautes." PLOS Genetics 17, no. 2 (February 18, 2021): e1009378. http://dx.doi.org/10.1371/journal.pgen.1009378.
Повний текст джерелаАнотація:
Mutations in RanBP2 (also known as Nup358), one of the main components of the cytoplasmic filaments of the nuclear pore complex, contribute to the overproduction of acute necrotizing encephalopathy (ANE1)-associated cytokines. Here we report that RanBP2 represses the translation of the interleukin 6 (IL6) mRNA, which encodes a cytokine that is aberrantly up-regulated in ANE1. Our data indicates that soon after its production, the IL6 messenger ribonucleoprotein (mRNP) recruits Argonautes bound to let-7 microRNA. After this mRNP is exported to the cytosol, RanBP2 sumoylates mRNP-associated Argonautes, thereby stabilizing them and enforcing mRNA silencing. Collectively, these results support a model whereby RanBP2 promotes an mRNP remodelling event that is critical for the miRNA-mediated suppression of clinically relevant mRNAs, such as IL6.
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Mili, Stavroula, Hong Jun Shu, Yingming Zhao, and Serafı́n Piñol-Roma. "Distinct RNP Complexes of Shuttling hnRNP Proteins with Pre-mRNA and mRNA: Candidate Intermediates in Formation and Export of mRNA." Molecular and Cellular Biology 21, no. 21 (November 1, 2001): 7307–19. http://dx.doi.org/10.1128/mcb.21.21.7307-7319.2001.
Повний текст джерелаАнотація:
ABSTRACT Nascent pre-mRNAs associate with hnRNP proteins in hnRNP complexes, the natural substrates for mRNA processing. Several lines of evidence indicate that hnRNP complexes undergo substantial remodeling during mRNA formation and export. Here we report the isolation of three distinct types of pre-mRNP and mRNP complexes from HeLa cells associated with hnRNP A1, a shuttling hnRNP protein. Based on their RNA and protein compositions, these complexes are likely to represent distinct stages in the nucleocytoplasmic shuttling pathway of hnRNP A1 with its bound RNAs. In the cytoplasm, A1 is associated with its nuclear import receptor (transportin), the cytoplasmic poly(A)-binding protein, and mRNA. In the nucleus, A1 is found in two distinct types of complexes that are differently associated with nuclear structures. One class contains pre-mRNA and mRNA and is identical to previously described hnRNP complexes. The other class behaves as freely diffusible nuclear mRNPs (nmRNPs) at late nuclear stages of maturation and possibly associated with nuclear mRNA export. These nmRNPs differ from hnRNPs in that while they contain shuttling hnRNP proteins, the mRNA export factor REF, and mRNA, they do not contain nonshuttling hnRNP proteins or pre-mRNA. Importantly, nmRNPs also contain proteins not found in hnRNP complexes. These include the alternatively spliced isoforms D01 and D02 of the hnRNP D proteins, the E0 isoform of the hnRNP E proteins, and LRP130, a previously reported protein with unknown function that appears to have a novel type of RNA-binding domain. The characteristics of these complexes indicate that they result from RNP remodeling associated with mRNA maturation and delineate specific changes in RNP protein composition during formation and transport of mRNA in vivo.
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Meaux, Stacie A., Christopher E. Holmquist, and William F. Marzluff. "Role of oligouridylation in normal metabolism and regulated degradation of mammalian histone mRNAs." Philosophical Transactions of the Royal Society B: Biological Sciences 373, no. 1762 (November 5, 2018): 20180170. http://dx.doi.org/10.1098/rstb.2018.0170.
Повний текст джерелаАнотація:
Metazoan replication-dependent histone mRNAs are the only known cellular mRNAs that are not polyadenylated. Histone mRNAs are present in large amounts only in S-phase cells, and their levels are coordinately regulated with the rate of DNA replication. In mammals, the stemloop at the 3′ end of histone mRNA is bound to stemloop binding protein, a protein required for both synthesis and degradation of histone mRNA, and an exonuclease, 3′hExo (ERI1). Histone mRNAs are rapidly degraded when DNA synthesis is inhibited in S-phase cells and at the end of S-phase. Upf1 is also required for rapid degradation of histone mRNA as is the S-phase checkpoint. We report that Smg1 is required for histone mRNA degradation when DNA replication is inhibited, suggesting it is the PI-like kinase that activates Upf1 for histone mRNA degradation. We also show that some mutant Upf1 proteins are recruited to histone mRNAs when DNA replication is inhibited and act as dominant negative factors in histone mRNA degradation. We report that the pathway of rapid histone mRNA degradation when DNA replication is inhibited in S-phase cells that are activating the S-phase checkpoint is similar to the pathway of rapid degradation of histone mRNA at the end of S-phase. This article is part of the theme issue ‘5′ and 3′ modifications controlling RNA degradation’.
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Kong, Jian, Marina Sumaroka, Dawn L. Eastmond та Stephen A. Liebhaber. "Shared Stabilization Functions of Pyrimidine-Rich Determinants in the Erythroid 15-lipoxygenase and α-globin mRNAs". Molecular and Cellular Biology 26, № 15 (1 серпня 2006): 5603–14. http://dx.doi.org/10.1128/mcb.01845-05.
Повний текст джерелаАнотація:
ABSTRACT The poly(C)-binding proteins, αCPs, comprise a set of highly conserved KH-domain factors that participate in mRNA stabilization and translational controls in developmental and viral systems. Two prominent models of αCP function link these controls to late stages of erythroid differentiation: translational silencing of 15-lipoxygenase (Lox) mRNA and stabilization of α-globin mRNA. These two controls are mediated via association of αCPs with structurally related C-rich 3′-untranslated region elements: the differentiation control elements (DICE) in Lox mRNA and the pyrimidine-rich motifs in α-globin mRNA. In the present report a set of mRNA translation and stability assays are used to determine how these two αCP-containing complexes, related in structure and position, mediate distinct posttranscriptional controls. While the previously reported translational silencing by the DICE is not evident in our studies, we find that the two determinants mediate similar levels of mRNA stabilization in erythroid cells. In both cases this stabilization is sensitive to interference by a nuclear-restricted αCP decoy but not by the same decoy restricted to the cytoplasm. These data support a general role for αCPs in stabilizing a subset of erythroid mRNAs. The findings also suggest that initial binding of αCP to target mRNAs occurs in the nucleus. Assembly of stabilizing mRNP complexes in the nucleus prior to export may maximize their impact on cytoplasmic events.
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Corcoran, Jennifer A., Wei-Li Hsu, and James R. Smiley. "Herpes Simplex Virus ICP27 Is Required for Virus-Induced Stabilization of the ARE-Containing IEX-1 mRNA Encoded by the Human IER3 Gene." Journal of Virology 80, no. 19 (October 1, 2006): 9720–29. http://dx.doi.org/10.1128/jvi.01216-06.
Повний текст джерелаАнотація:
ABSTRACT Herpes simplex virus (HSV) stifles cellular gene expression during productive infection of permissive cells, thereby diminishing host responses to infection. Host shutoff is achieved largely through the complementary actions of two viral proteins, ICP27 and virion host shutoff (vhs), that inhibit cellular mRNA biogenesis and trigger global mRNA decay, respectively. Although most cellular mRNAs are thus depleted, some instead increase in abundance after infection; perhaps surprisingly, some of these contain AU-rich instability elements (AREs) in their 3′-untranslated regions. ARE-containing mRNAs normally undergo rapid decay; however, their stability can increase in response to signals such as cytokines and virus infection that activate the p38/MK2 mitogen-activated protein kinase (MAPK) pathway. We and others have shown that HSV infection stabilizes the ARE mRNA encoding the stress-inducible IEX-1 mRNA, and a previous report from another laboratory has suggested vhs is responsible for this effect. However, we now report that ICP27 is essential for IEX-1 mRNA stabilization whereas vhs plays little if any role. A recent report has documented that ICP27 activates the p38 MAPK pathway, and we detected a strong correlation between this activity and stabilization of IEX-1 mRNA by using a panel of HSV type 1 (HSV-1) isolates bearing an array of previously characterized ICP27 mutations. Furthermore, IEX-1 mRNA stabilization was abrogated by the p38 inhibitor SB203580. Taken together, these data indicate that the HSV-1 immediate-early protein ICP27 alters turnover of the ARE-containing message IEX-1 by activating p38. As many ARE mRNAs encode proinflammatory cytokines or other immediate-early response proteins, some of which may limit viral replication, it will be of great interest to determine if ICP27 mediates stabilization of many or all ARE-containing mRNAs.
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Hsu, Wei-Li, Holly A. Saffran, and James R. Smiley. "Herpes Simplex Virus Infection Stabilizes Cellular IEX-1 mRNA." Journal of Virology 79, no. 7 (April 1, 2005): 4090–98. http://dx.doi.org/10.1128/jvi.79.7.4090-4098.2005.
Повний текст джерелаАнотація:
ABSTRACT Herpes simplex virus (HSV) virion host shutoff protein (vhs) destabilizes cellular and viral mRNAs. Previous work from several laboratories has indicated that vhs accelerates the turnover of most host mRNAs and provided evidence that at least some of these are degraded via endonucleolytic cleavage near regions of translational initiation followed by 5′→3′ decay. In contrast, several recent reports have argued that vhs is selective, preferentially targeting a subset of mRNAs including some that bear AU-rich instability elements (such as the stress-inducible IEX-1 mRNA). These reports concluded that vhs triggers deadenylation, 3′ cleavage, and 3′→5′ decay of IEX-1 mRNA. However, we report here that HSV infection does not increase the rate of degradation of IEX-1 mRNA; rather, actinomycin D chase assays indicate that the transcript is stabilized relative to that in uninfected cells in both the presence and absence of functional vhs. Moreover, deadenylated but otherwise intact IEX-1 mRNA was readily detected in uninfected cells cultured under our experimental conditions, and its relative abundance did not increase following HSV type 1 (HSV-1) infection. We confirm that HSV infection increases the relative abundance of a discrete 0.75-kb 3′-truncated IEX-1 RNA species in a vhs-dependent manner. This truncated transcript was also detected (albeit at lower levels) in cells infected with vhs mutants and in uninfected cells, where it increased in abundance in response to tumor necrosis factor alpha, cycloheximide, and puromycin. We conclude that IEX-1 mRNA is not preferentially degraded during HSV-1 infection and that HSV-1 instead inhibits the normal turnover of this mRNA.
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Kay, M. A., and M. Jacobs-Lorena. "Selective translational regulation of ribosomal protein gene expression during early development of Drosophila melanogaster." Molecular and Cellular Biology 5, no. 12 (December 1985): 3583–92. http://dx.doi.org/10.1128/mcb.5.12.3583.
Повний текст джерелаАнотація:
We have previously characterized a cloned cDNA coding for a developmentally regulated mRNA in Drosophila melanogaster whose expression is selectively regulated at the translational level during oogenesis and embryogenesis. In this report we show that this translationally regulated mRNA (rpA1) codes for an acidic ribosomal protein. Furthermore, our results indicate that most ribosomal protein mRNAs are regulated similarly to rpA1 mRNA. This conclusion is based on cell-free translation of mRNAs derived from polysomes and postpolysomal supernatants as well as in vivo labeling experiments. Thus, the translation of many ribosomal protein mRNAs appears to be temporally related to the synthesis of rRNA during D. melanogaster development. The relationship between rRNA transcription and ribosomal protein mRNA translation was further investigated by genetically reducing rRNA synthesis with the use of bobbed mutants. Unexpectedly, neither ribosomal protein mRNA abundance nor translation was altered in these mutants.
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Kay, M. A., and M. Jacobs-Lorena. "Selective translational regulation of ribosomal protein gene expression during early development of Drosophila melanogaster." Molecular and Cellular Biology 5, no. 12 (December 1985): 3583–92. http://dx.doi.org/10.1128/mcb.5.12.3583-3592.1985.
Повний текст джерелаАнотація:
We have previously characterized a cloned cDNA coding for a developmentally regulated mRNA in Drosophila melanogaster whose expression is selectively regulated at the translational level during oogenesis and embryogenesis. In this report we show that this translationally regulated mRNA (rpA1) codes for an acidic ribosomal protein. Furthermore, our results indicate that most ribosomal protein mRNAs are regulated similarly to rpA1 mRNA. This conclusion is based on cell-free translation of mRNAs derived from polysomes and postpolysomal supernatants as well as in vivo labeling experiments. Thus, the translation of many ribosomal protein mRNAs appears to be temporally related to the synthesis of rRNA during D. melanogaster development. The relationship between rRNA transcription and ribosomal protein mRNA translation was further investigated by genetically reducing rRNA synthesis with the use of bobbed mutants. Unexpectedly, neither ribosomal protein mRNA abundance nor translation was altered in these mutants.
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Chen, Qiang, Sujatha Jagannathan, David W. Reid, Tianli Zheng, and Christopher V. Nicchitta. "Hierarchical regulation of mRNA partitioning between the cytoplasm and the endoplasmic reticulum of mammalian cells." Molecular Biology of the Cell 22, no. 14 (July 15, 2011): 2646–58. http://dx.doi.org/10.1091/mbc.e11-03-0239.
Повний текст джерелаАнотація:
The mRNA transcriptome is currently thought to be partitioned between the cytosol and endoplasmic reticulum (ER) compartments by binary selection; mRNAs encoding cytosolic/nucleoplasmic proteins are translated on free ribosomes, and mRNAs encoding topogenic signal-bearing proteins are translated on ER-bound ribosomes, with ER localization being conferred by the signal-recognition particle pathway. In subgenomic and genomic analyses of subcellular mRNA partitioning, we report an overlapping subcellular distribution of cytosolic/nucleoplasmic and topogenic signal-encoding mRNAs, with mRNAs of both cohorts displaying noncanonical subcellular partitioning patterns. Unexpectedly, the topogenic signal-encoding mRNA transcriptome was observed to partition in a hierarchical, cohort-specific manner. mRNAs encoding resident proteins of the endomembrane system were clustered at high ER-enrichment values, whereas mRNAs encoding secretory pathway cargo were broadly represented on free and ER-bound ribosomes. Two distinct modes of mRNA association with the ER were identified. mRNAs encoding endomembrane-resident proteins were bound via direct, ribosome-independent interactions, whereas mRNAs encoding secretory cargo displayed predominantly ribosome-dependent modes of ER association. These data indicate that mRNAs are partitioned between the cytosol and ER compartments via a hierarchical system of intrinsic and encoded topogenic signals and identify mRNA cohort-restricted modes of mRNA association with the ER.
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Proulex, Grayson C. R., Marcus J. Meade, Kalina M. Manoylov, and A. Bruce Cahoon. "Mitochondrial mRNA Processing in the Chlorophyte Alga Pediastrum duplex and Streptophyte Alga Chara vulgaris Reveals an Evolutionary Branch in Mitochondrial mRNA Processing." Plants 10, no. 3 (March 18, 2021): 576. http://dx.doi.org/10.3390/plants10030576.
Повний текст джерелаАнотація:
Mitochondria carry the remnant of an ancestral bacterial chromosome and express those genes with a system separate and distinct from the nucleus. Mitochondrial genes are transcribed as poly-cistronic primary transcripts which are post-transcriptionally processed to create individual translationally competent mRNAs. Algae post-transcriptional processing has only been explored in Chlamydomonas reinhardtii (Class: Chlorophyceae) and the mature mRNAs are different than higher plants, having no 5′ UnTranslated Regions (UTRs), much shorter and more variable 3′ UTRs and polycytidylated mature mRNAs. In this study, we analyzed transcript termini using circular RT-PCR and PacBio Iso-Seq to survey the 3′ and 5′ UTRs and termini for two green algae, Pediastrum duplex (Class: Chlorophyceae) and Chara vulgaris (Class: Charophyceae). This enabled the comparison of processing in the chlorophyte and charophyte clades of green algae to determine if the differences in mitochondrial mRNA processing pre-date the invasion of land by embryophytes. We report that the 5′ mRNA termini and non-template 3′ termini additions in P. duplex resemble those of C. reinhardtii, suggesting a conservation of mRNA processing among the chlorophyceae. We also report that C. vulgaris mRNA UTRs are much longer than chlorophytic examples, lack polycytidylation, and are polyadenylated similar to embryophytes. This demonstrates that some mitochondrial mRNA processing events diverged with the split between chlorophytic and streptophytic algae.
Дисертації з теми "MRNA report"
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SILVA, Adalúcia da. "Utilização de um repórter fluorescente para a avaliação funcional de fatores envolvidos na iniciação da tradução de tripanossomatídeos." Universidade Federal de Pernambuco, 2016. https://repositorio.ufpe.br/handle/123456789/19542.
Повний текст джерелаАнотація:
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FACEPE
Os tripanossomatídeos são parasitas flagelados causadores de diversas doenças humanas e que apresentam algumas características moleculares bem distintas. Nestes organismos, o controle da sua expressão gênica é quase exclusivamente pós-transcricional e dados obtidos até o momento sugerem que a etapa de iniciação da tradução tem um papel importante neste controle. Durante a iniciação da tradução de eucariotos, o complexo trimérico eIF4F (formado pelas subunidades eIF4A, eIF4G e eIF4E) tem uma participação importante no reconhecimento do mRNA, facilitando o recrutamento ribossomal para iniciar a síntese proteica. Múltiplos homólogos das subunidades do complexo eIF4F foram identificados em tripanossomatídeos, mas não foi possível elucidar a função específica de cada um deles. Desta forma, este trabalho teve como objetivo o desenvolvimento de um ensaio in vivo para a avaliação do efeito da superexpressão desses homólogos de Trypanosoma brucei na tradução de um mRNA repórter, o qual codifica a proteína GFP (green fluorescent protein). Três linhagens repórter foram obtidas (4212, 4213 e 4235) e sete homólogos das subunidades do complexo eIF4F e um de PABP foram testados nestas linhagens. A análise da superexpressão foi feita através de ensaios de Western blot, seus perfis de crescimento foram avaliados por curvas de crescimento e a expressão de GFP foi avaliada através de citometria de fluxo. Os resultados apresentados mostram que as linhagens repórter 4213 e 4235 são viáveis para serem utilizadas na caracterização das proteínas envolvidas no controle da expressão gênica de tripanossomatídeos. Foi observado que as proteínas EIF4E1 e EIF4E2 provocam diminuição do crescimento, enquanto a superexpressão de EIF4E3, EIF4E4, EIF4E4W279A, EIF4G3, EIF4G4 e PABP1 não alteram o crescimento celular dos parasitas. Apesar da detecção de variações na expressão de GFP durante a superexpressão de alguns dos homólogos testados, não foi possível, contudo, confirmar que estas proteínas aumentam ou diminuem a tradução. Ensaios complementares ainda precisam ser feitos para confirmar a real função destes.
The trypanosomatids are flagellated parasites responsible for several human diseases and which display unique molecular characteristics. Control of gene expression in these organisms is almost exclusively post-transcriptional and the data generated so far suggest that the initiation stage of translation plays an important role in this control. During translation initiation in eukaryotes, the trimeric complex eIF4F (formed by the eIF4A eIF4G and eIF4E subunits) has a relevant role in mRNA recognition and facilitates ribosomal recruitment to start the protein synthesis. Multiples homologues for the eIF4F subunits have been identified in trypanosomes, but it has not been possible to elucidate their specific functions. Thus, this study aimed to develop an in vivo assay to evaluate the effect of the overexpression of these Trypanosoma brucei homologues during the translation of a reporter mRNA encoding for the green fluorescent protein (GFP). Three reporter strains were generated (4212, 4213 and 4235) in which seven homologues of eIF4F subunits and one of their PABP partner were overexpressed. Confirmation of overexpression was carried out by Western blot assays, growth profiles were evaluated by cell counting and GFP expression was assessed by flow cytometry. The results generated show that the reporter lines 4213 and 4235 are feasible for use in the characterization of proteins involved in the control of trypanosomatids gene expression. Overexpression of EIF4E1 and EIF4E2 was seen to induce a reduction in cell growth, while EIF4E3, EIF4E4, EIF4E4W279A, EIF4G3, EIF4G4 and PABP1 did not interfere with growh. Despite the detection of variations in GFP expression during overexpression of some of the homologues tested it was not possible to confirm if these proteins increase or decrease translation. Complementary assays still need to be done to confirm the true function of these factors.
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Siboni, Ruth. "Characterization of Small Molecules that Reduce CUG Repeat RNA in Myotonic Dystrophy." Thesis, University of Oregon, 2015. http://hdl.handle.net/1794/19260.
Повний текст джерелаАнотація:
Myotonic dystrophy (DM) is an inherited disease characterized by myotonia, insulin resistance, cardiomyopathy, and cognitive deficiencies. DM is a triplet repeat disorder, meaning that affected individuals carry anywhere between 50 and thousands of CTG/CCTG repeats in their genetic makeup. When transcribed into RNA, these repeats become “toxic” in the sense that they serve to bind and sequester important RNA binding proteins. One such family of proteins, the Muscleblind-like (MBNL) family, is important in the regulation of alternative mRNA splicing, and thus the sequestration of MBNL proteins leads to a number of mis-splicing events. Many of these events are directly correlated to DM symptoms.
While there is no known cure for DM, the use of small molecules to treat symptoms is a well-characterized therapeutic tactic with immense promise. Pentamidine is a small molecule that was found to reverse mis-splicing in both DM cell and mouse models. Mechanistically, this molecule is particularly unique because unlike many small molecules, which physically displace MBNL from the toxic CUG RNA, pentamidine reduces CUG RNA levels, possibly through inhibition of CTG transcription.
Chapter I summarizes alternative splicing mechanisms and regulation, defines MBNL protein structure and function, describes DM pathophysiology and molecular mechanism, and finally provides an overview of pentamidine characterization as a small molecule therapeutic. Chapter II reports the development of an in vitro T7 transcription assay, which allowed us to compare the relative efficacy by which pentamidine is able to inhibit the transcription of various repeat and non-repeat DNA sequences. This chapter further reports the characterization of a series of methylene linker analogues of pentamidine, which were also characterized through the T7 transcription assay. Chapter III details our thorough structure-activity relationship investigation of bisbenzamidine analogues of pentamidine, both in in vivo and in vitro models. Chapter IV describes our characterization of actinomycin D, a known transcription inhibitor and chemotherapeutic, within the DM disease framework. Chapter V summarizes these data, which ultimately serve as a proof of concept for the potential of CTG transcription inhibition in therapeutic contexts and broadly describe their application in other repeat diseases.
This dissertation contains previously published and unpublished co-authored material.10000-01-01
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Ezzatizadeh, Vahid. "Friedreich ataxia : investigating the relationships between mismatch repair gene expression, FXN gene expression and GAA repeat instability in human and mouse cells and tissues." Thesis, Brunel University, 2012. http://bura.brunel.ac.uk/handle/2438/7626.
Повний текст джерелаАнотація:
Friedreich ataxia (FRDA) is the most common inherited ataxia disorder, caused by a GAA repeat expansion mutation within the first intron of the FXN gene. The subsequent deficiency of frataxin protein leads to neurological disability, increased risk of diabetes mellitus, cardiomyopathy and premature death. The exact FRDA disease mechanism is not yet clear, despite some understanding of epigenetic, transcriptional and DNA repair system effects that lead to frataxin reduction. Previous studies have shown that mismatch repair (MMR) genes can affect other trinucleotide repeat disorders by destabilisation of the repeats. Furthermore, it has been proposed that frataxin deficiency might lead to cell malignancy by an as yet undefined mode of action. Therefore, the principle aim of this thesis was to use human and genetically altered mouse cells and tissues to understand the effects of MMR proteins on GAA repeat instability and FXN transcription, and also to identify potential changes in MMR transcription that might cause malignancy in FXN-defective human cells. Firstly, by using FXN and MMR genetically altered mice, MMR proteins were shown to be involved in both intergenerational and somatic GAA repeat instability, although their effects in the two systems were different. Thus, Msh2 or Msh3 were both found to protect against intergenerational transmission of GAA contractions, while loss of Msh2 or Msh3 reduced somatic GAA repeat expansions and increased levels of FXN transcription in brain and cerebellum tissues. Loss of Msh6 induced both intergenerational GAA repeat expansions and contractions, while the frequency of somatic GAA repeat expansions was reduced. Curiously, the level of FXN transcription was also reduced in Msh6-deficient brain and cerebellum tissues. On the other hand, Pms2 was found to protect against both intergenerational and somatic GAA repeat expansions, with loss of Pms2 causing increased GAA repeat expansions and decreased levels of FXN transcription in brain and cerebellum tissues. Finally, loss of Mlh1 led to a reduced frequency of both intergenerational and somatic GAA repeat expansions, but the level of FXN transcription was also reduced in brain and cerebellum tissues. Furthermore, upregulation of MMR mRNA expression was detected in human FRDA fibroblast cells, but downregulation was seen in FRDA cerebellum tissues, suggesting tissue-dependent control of FXN and MMR expression. In summary, these studies indicate that the MMR system can affect GAA repeat expansion instability and FXN transcription through different mechanisms of action. Furthermore, frataxin deficiency can also affect the levels of MMR mRNA expression in a tissue-dependent manner. These findings will assist future investigations aimed at identifying novel FRDA therapies.
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Ly, Socheata. "Investigating the Role of Mutant Huntingtin mRNA in Huntington’s Disease." eScholarship@UMMS, 2020. https://escholarship.umassmed.edu/gsbs_diss/1117.
Повний текст джерелаАнотація:
Mutant mRNA and protein both contribute to the clinical manifestation of many repeat-associated neurodegenerative and neuromuscular disorders. The presence of nuclear RNA clusters is a feature shared amongst these diseases, such as C9ORF72/ALS and myotonic dystrophy 1/2 (DM1/2); however, this pathological hallmark has not been conclusively demonstrated in Huntington’s disease (HD) in vivo. Investigations into HD – caused by a CAG repeat expansion in exon 1 of the huntingtin (HTT) gene – have largely focused on toxic protein gain-of-function as a disease-causing feature, with fewer studies investigating the role of mutant HTT mRNA in pathology or pathogenesis.
Here we report that in two HD mouse models, YAC128 and BACHD-97Q-ΔN17, mutant HTT mRNA is preferentially retained in the nucleus in vivo. Furthermore, we observed the early, widespread formation of large mutant HTT mRNA clusters (approximately 0.6 to 5 µm3 in size) present in over 50-75% of striatal and cortical neurons. Affected cells were limited to one cluster at most. Endogenous wild-type mouse Htt or human HTT mRNA containing 31 or fewer repeats did not form clusters. Additionally, the aberrantly spliced N-terminal exon 1-intron 1 RNA fragment, HTT1a, also formed clusters that fully co-localized with the mutant HTT mRNA clusters. These results suggest that multiple repeat-containing transcripts can coalesce to form a single cluster in a given cell. Treating YAC128 mice with antisense oligonucleotides efficiently silenced individual HTT mRNA foci but had limited impact on clusters. Our findings identify mutant HTT mRNA clustering as an early, robust molecular signature of HD, further supporting HD as a repeat expansion disease with suspected mRNA involvement.
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Huo, Wenwen. "Quantification of replication present in HIV reports and effect of patient movement between wards on MRSA infection." Thesis, University of Strathclyde, 2014. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=23667.
Повний текст джерелаАнотація:
Outbreaks of widely spread infectious diseases, such as Human Immunodefficiency Virus (HIV), Severe Acute Respiratory Syndrome (SARS) and Swineflu (H1N1) and hospital acquired infections, such as Meticillin-resistant Staphylococcus Aureus (MRSA) and Clostridium Diffcile, are serious health problems which have been tackled by the World Health Organization and international health protection agencies. Various statistical analyses have contributed a remarkable effect on providing scientific evidence on which to base political decisions and infection control strategies. In this project, we focused on two infectious diseases: HIV and MRSA and the research project is divided into two separate parts. One is the quantification of replication in HIV anonymous test reports and the other is the effect of patient movement between wards on the acquisition of MRSA. The first research project is concerned with the analysis of an anonymous HIV test dataset. The data is collected as a set of birthdays and it is possible that there is repeated sampling of the same person. The aim is to quantify the amount of replication in the HIV data using a maximum likelihood technique and then give the confidence intervals for the estimated amount of replication using the bootstrap method. The data were provided by the Public Health Laboratory Service (PHLS), Colindale, London in 1994, who were interested in a statistical method to estimate multiple counting that possibly existed in the database. The data consists of individual records of the number of AIDS cases diagnosed, with birthdates from 1901 to 1973. There were two datasets provided by the PHLS, one of which contained 1,134 records and was provided in 1991. The other dataset was provided in 1994 with the sample size 17,137. An estimate of the true number of distinct individuals as well as the percentage of replication was obtained by programming the maximum likelihood calculation in the languages R and C. This technique is based upon evaluation of the probability that two records with the same birthdate represent two separate individuals as opposed to the same person reported twice. The results for the 1991 dataset showed that there were five out of sixteen birth years (i.e. 31.25% of the observed records in the 1991 dataset) with replication in the true number of distinct individuals. In the results of the 1994 dataset, the majority of the birth years (57/73) recorded the correct number of distinct individuals in the observations. The 95% confidence intervals for the estimated amount of replication were calculated by applying a parametric bootstrap method. The results show that the birth years in the 1991 dataset with non-zero estimated amount of replication (the birth years of 1931, 1934, 1935, 1943 and 1944) have comparatively wide 95% bootstrap confidence intervals, which implies higher uncertainty of the true amount of replication. A similar conclusion was obtained from the results of 95% bootstrap con dence intervals for the 1994 dataset. Comparing the results within the same birth years recorded in the 1991 dataset and the 1994 dataset, the data indicate that the confidence intervals for the 1994 dataset are mainly narrower than the corresponding ones in the 1991 dataset. The conclusion of this study illustrates the drawback of recording the HIV patients only with date of birth, which has now been improved by combining with 'Soundex' codes for the surname and gender. The second part of the project aims to estimate the impact of patient movement within a hospital on the risk of MRSA acquisition by using data from the MRSA screening admission and discharge studies in Scotland which took place in two hospitals in 2010. The data consist of an admission-only database (7,181 patients), a discharge-only database (2,432 patients) and a combined admission-discharge cohort (2,792 patients). The third database has complete information on MRSA status on admission, on discharge, as well as data on the wards the patient was in while in hospital. In order to understand the effect of potential risk factors on MRSA acquisition, a multivariate logistic regression model was constructed to analyse the effects of the number of wards a patient was in on MRSA acquisition as well as other risk factors. Receiver Operating Characteristic (ROC) curves were plotted and the individual area under the curve (AUC) was also calculated for indicating the reliability and the accuracy of the prediction of the models. Furthermore, we modelled the dynamic patient movement and assessed the effect of being in a ward with MRSA by imputing the unknown date of transfer, simulating the missing length of stay (along with the simulation envelope). The timelines of MRSA infection and carriage pressure in each ward of the two hospitals were then mapped for all patients in the three databases, imputing where necessary. Patient movement was measured as a volume indicator in terms of the frequency of ward to ward transfer and as cohabiting in the same ward. By using logistic regression within a bootstrap simulation, we estimated the odds ratio of acquisition of MRSA association with being in a ward with MRSA present, which was given by averaging the estimated effects from the fitted models, and generating the 95% confidence intervals. The results indicate that the number of wards that patients had moved through and patients being in a ward with MRSA present do not affect the risk of acquiring MRSA significantly over and above the patient level risk factors such as age and the presence of open wounds or catheters. Some further work which can be done in an MRSA screening programme is suggested as an implementation study.
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Thieme, René, Susanne Kurz, Marlen Kolb, Tewodros Debebe, Susanne Holtze, Michaela Morhart, Klaus Huse, et al. "Analysis of alpha-2 macroglobulin from the long-lived and cancer-resistant naked mole-rat and human plasma." Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-175598.
Повний текст джерелаАнотація:
Background: The naked mole-rat (NMR) is a long-lived and cancer resistant species. Identification of potential anti-cancer and age related mechanisms is of great interest and makes this species
eminent to investigate anti-cancer strategies and understand aging mechanisms. Since it is known that the NMR expresses higher liver mRNA-levels of alpha 2-macroglobulin than mice, nothing is known about its structure, functionality or expression level in the
NMR compared to the human A2M.
Results: Here we show a comprehensive analysis of NMR- and human plasma-A2M, showing a different prediction in glycosylation of NMR-A2M, which results in a higher molecular weight compared to human A2M. Additionally, we found a higher concentration of A2M (8.3±0.44 mg/mL vs. and 4.4±0.20 mg/mL) and a lower total plasma protein content (38.7±1.79 mg/mL vs. 61.7±3.20 mg/mL) in NMR compared to human. NMR-A2M can be transformed by
methylamine and trypsin resulting in a conformational change similar to human A2M. NMRA2M is detectable by a polyclonal antibody against human A2M. Determination of tryptic and anti-tryptic activity of NMR and human plasma revealed a higher anti-tryptic activity of the NMR plasma. On the other hand, less proteolytic activity was found in NMR plasma compared to human plasma.
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Rohani, Leili, Claire Fabian, Heidrun Holland, Yahaira Naaldijk, Ralf Dressel, Henry Löffler-Wirth, Hans Binder, A. Arnold, and Alexandra Stolzing. "Generation of human induced pluripotent stem cells using non-synthetic mRNA." Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-205889.
Повний текст джерелаАнотація:
Here we describe some of the crucial steps to generate induced pluripotent stemcells (iPSCs) usingmRNA transfection. Our approach uses a V. virus-derived capping enzyme instead of a cap-analog, ensuring 100% proper cap orientation for in vitro transcribedmRNA. V. virus\' 2′-O-Methyltransferase enzymecreates a cap1 structure found in higher eukaryotes and has higher translation efficiency compared to other methods. Use of the polymeric transfection reagent polyethylenimine proved superior to other transfection methods. The mRNA created via this method did not trigger an intracellular immune response via human IFN-gamma (hIFN-γ) or alpha (hIFN-α) release, thus circumventing the use of suppressors. Resulting mRNA and protein
were expressed at high levels for over 48 h, thus obviating daily transfections. Using this method, we demonstrated swift activation of pluripotency associated genes in human fibroblasts. Low oxygen conditions further facilitated colony formation. Differentiation into different germ layers was confirmed via teratoma assay. Reprogramming with non-synthetic mRNA holds great promise for safe generation of iPSCs of human origin. Using the protocols described herein we hope to make this method more accessible to other groups as a fast, inexpensive, and non-viral reprogramming approach.
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Pastic, Alyssa. "LRRK2 Phosphorylates HuD to Affect the Post-Transcriptional Regulation of Parkinson's Disease-Linked mRNA Targets." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/38593.
Повний текст джерелаАнотація:
Parkinson's Disease (PD) is a late-onset neurodegenerative disease characterized by progressive motor dysfunction caused by a loss of dopaminergic neurons for which there is no known cure. Among the most common genetic causes of PD are mutations in the leucine-rich repeat kinase 2 gene (LRRK2), encoding a multi-domain protein with kinase activity. The LRRK2 G2019S mutation causes hyperactivity of the kinase domain and is the most frequent LRRK2 mutation in patients with familial PD, though its role in PD pathology remains unclear. Preliminary data from the lab of our collaborator, Dr. David Park, demonstrated through a genetic screen in Drosophila melanogaster that the deletion of rbp9 encoding an RNA-binding protein prevented pathology induced by PD-relevant mutations in the LRRK2 kinase domain. The neuronal homolog of RBP9 in humans is HuD, a member of the Hu family of RNA-binding proteins that regulates the expression of many transcripts involved in neuronal development, plasticity, and survival. In addition, HuD has been shown to modify the age-at-onset or risk of developing PD. Here, we studied the effect of LRRK2 on the post-transcriptional regulation of mRNAs bound by HuD in the context of PD. Our findings showed that HuD is a substrate for LRRK2 phosphorylation in vitro, and that LRRK2 G2019S hyperphosphorylates HuD. We demonstrated that LRRK2 kinase activity is required for the binding of several transcripts by HuD that encode PD-relevant proteins such as α-synuclein and neuronal survival factor BDNF. Our findings in human neuroblastoma cells indicated that LRRK2 regulates the protein levels of HuD mRNA targets α-synuclein and BDNF in a mechanism that can by modified by HuD. Finally, we showed that the combination of HuD knockout with LRRK2 G2019S expression in mice rescues aberrant expression of HuD targets in mice with only the LRRK2 G2019S mutation or the knockout of HuD alone. Together, our findings demonstrate that LRRK2 affects the post-transcriptional regulation of HuD-bound mRNAs, and suggest the use of HuD as a potential therapeutic target in patients with PD caused by the LRRK2 G2019S mutation.
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Zapfe, Luise. "mRNA-Expression von Genen des Fett- und Kohlenhydratstoffwechsels unterschiedlicher Fettlokalisationen bei Kühen." Doctoral thesis, Universitätsbibliothek Leipzig, 2010. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-62426.
Повний текст джерелаАнотація:
Problemstellung: Die Tiergesundheit hat sich bei Milchkühen in den letzten Jahren weltweit negativ entwickelt. Wichtigster Ausdruck dafür ist die auf ca. 2,4 Jahre verkürzte Nutzungsdauer. Dabei spielt das Fettmobilisationssyndrom eine dominante Rolle. Das Fettgewebe ist nicht nur als reiner Energiespeicher, sondern als endokrines stoffwechselaktives Organ anzusehen. Untersuchungen an Menschen und Mäusen haben gezeigt, dass das Fettgewebe in Abhängigkeit von seiner Lokalisation im Körper unterschiedlich auf metabolische und hormonelle Stimuli reagiert. Es gibt Hinweise, dass auch für das Rind ähnliche Differenzen angenommen werden können. Zielstellung: Um die Eigenschaften des bovinen Fettgewebes und seine Rolle im Energiestoffwechsel besser charakterisieren zu können, war das Ziel der vorliegenden Untersuchung, die mRNA-Expressionen ausgewählter für den Fettstoffwechsel relevante Gene im bovinen Fettgewebe an verschiedenen Lokalisationen grundlegend in gesunden Rindern zu untersuchen. Material und Methoden: Die Probenentnahme erfolgte an 12 gesunden Schlachtkühen direkt nach der Tötung, die aufgrund Schwermelkbarkeit oder Unfruchtbarkeit geschlachtet wurden. Das Fettgewebe wurde aus dem Omentum majus, dem Depotfett der Niere, im kaudalen Beckendrittel (retroperitoneales Fett), dem Hüftbereich (subkutanes Fett) und dem Fett an der Herzbasis entnommen. Die Proben wurden in Flüssigstickstoff tiefgefroren, auf Trockeneis transportiert und bis zur Untersuchung bei -70°C gelagert. Die mRNA-Expression für die verschiedenen Gene (Hormonsensitive Lipase (HSL), Lipoproteinlipase (LPL), Fettsäuresynthase (FASN), Leptin, Adiponektin, Retinolbindungsprotein 4 (RBP4), Tumornekrosefaktor (TNF) und Interleukin 6 (IL-6), Fettsäurebindungsproteine (FABP3, 4 und 5) und Glukosetransporter 4 (GLUT4)) , wurden mit einer quantitativen real time (RT)-PCR gemessen. Ergebnisse: Die mRNA-Expressionen der verschiedenen oben genannten Gene, ausgenommen IL-6 und FABP3, sind im bovinen Fettgewebe nachweisbar. Die mRNA-Expressionen unterschieden sich in den einzelnen Fettdepots nicht signifikant. Ausnahme hierbei bildete RBP4, dessen mRNA im pericardialen Fett signifikant höher exprimiert war als im subkutanen und omentalen Depot. Die mRNA-Expression des subkutanen, omentalen, perirenalen und pericardialen Fettdepots korrelierten signifikant positive untereinander. Schlussfolgerung: Die mRNA-Expressionen der in den Fettstoffwechsel involvierten und untersuchten Gene gesunder Rinder waren nachweisbar, unterschieden sich jedoch nicht signifikant von einander mit Ausnahme der RBP4 mRNA. Die positiven signifikanten Korrelationen zwischen dem subkutanen, omentalen, perirenalen und pericardialen Fettlokalisationen und gleichmäßigen Expressionen innerhalb der Gewebe deuten auf eine einheitliche Fettmetabolismus des gesamten Körpers. Verglichen mit Ergebnissen der Humanmedizin sind nur wenige Übereinstimmungen (HSL, LPL, GLUT4,TNF) zu eruieren. Weitere Studien mit gesunden Tieren im Vergleich zu erkrankten Rindern müssen klären, ob eine mögliche Verschiebung der mRNA-Konzentrationen auf das Fettmobilisationssyndrom hinweisenPurpose: Over the last years, the situation of animal health concerning dairy cows has developed worldwide in an adverse way. Most important indicator is the shortened useful life of approx. 2.4 years. The fat mobilization syndrome plays a dominant role in this process. Apparently, fatty tissue does not only serve as a mere energy reservoir, but also as an endocrin organ with metabolic activity. Researches on humans and mice have shown fatty tissue to react on metabolic and hormonal stimuli in different ways, depending on its body localization. There are dues to anticipate, similar differences in cattle. Objectives: In order to better characterize the attributes of bovine fatty tissue and its purpose in metabolism, the present study aims examine basically the expression of mRNA in selected genes which are important for lipid metabolism in bovine fatty tissue of different localizations in healthy cattle. Methods and material: Samples where taken from twelve carcasses of healthy dairy cows slaughtered for reason of difficult milking or infertility directly after killing. Fatty tissue was taken from omentum major, kidney capsula, caudal pelvis area (retroperiteonal fat), hip area (subcutaneous fat), and cardiac base. It was instantly quick-freezed in liquid nitrogen, put on dry ice while transporting, and stored at -70°C until analysis. The expression of mRNA of different genes (hormone-sensitive lipase (HSL), lipoproteine lipase (LPL), fatty acid synthase (FASN), fatty acid binding proteine (FABP3,4 and 5), retinol binding proteine 4 (RBP4), adiponectine, glucose transporter 4 (GLUT4), leptin, interleukin-6 (IL-6), and tumor necrosis factor a (TNFα) was measured by means of a quantitative real-time (RT)-PCR. Results: The mRNA-expressions of all these different genes except IL-6 and FABP3 were detected in bovine fatty tissue. The differences of mRNA-expression between sample localization were not statistically significant. RBP4 was excepted, which mRNA showed a significantly higher expression in pericardial fat than in subcutaneous and omental fat, respectively. The correlation between mRNA-expressions of subcutaneous, omental, pericardial and perirenal fat was significant. Conclusions: The mRNA-expression of examined genes being involved in fatty tissue metabolism, were detected in healthy cattle, but were not significantly different, except RBP4. Significantly positive correlations between subcutaneous, omental, perirenal and pericardial localization and consistent expression indicate an integrative metabolism of the whole body. Compared to results of the human medicine only few analogies (HSL, LPL, GLUT4, TNF) were found. Further studies comparing healthy and diseased cattle will have to prove, if possible displacements of the mRNA-level can indicate the fat mobilization syndrome being present
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Jarrige, Domitille. "Déchiffrer le "code OPR" pour une meilleure compréhension du rôle physiologique des protéines OPR." Electronic Thesis or Diss., Sorbonne université, 2019. http://www.theses.fr/2019SORUS632.
Повний текст джерелаАнотація:
À la suite de l’endosymbiose, le génome chloroplastique a rétréci et dépend maintenant du génome nucléaire pour son expression. Chez Chlamydomonas reinhardtii, les protéines Octotricopeptide repeat (OPR), codées dans le noyau, contrôlent l’expression d’ARNm chloroplastiques spécifiques. La répétition OPR est un motif dégénéré de 38 acides aminés, qui forme un tandem d’hélices α antiparallèles qui lient l’ARN. Une répétition OPR est prédite pour interagir avec un nucléotide spécifique grâce à des résidus variables à des positions précises. La succession de répétitions permet aux protéines OPR de se lier à une séquence donnée. En partant d’un « code OPR » théorique, j’ai cherché à étudier cette spécificité de reconnaissance. J’ai mute in vivo les cibles chloroplastiques de facteurs OPR pour empêcher l’interaction OPR/ARN, puis j’ai tenté de la restaurer en mutant les résidus conférant la spécificité dans les répétitions correspondantes. Étonnamment, les interactions OPR/ARN sont très résilientes, ce qui a complétement changé notre vision de ces interactions in vivo. Des études fonctionnelles complémentaires que j’ai réalisées sur les facteurs OPR MDB1 and MTHI1 ont révélé que l’expression des gènes chloroplastiques dépend probablement de systèmes de facteurs nucléaires. En coopérant ces facteurs auraient une affinité combinée plus forte et seraient ainsi plus résilientsFollowing endosymbiosis, the chloroplast genome shrunk and became reliant on the host genome for its expression. In Chlamydomonas reinhardtii, Octotricopeptide repeat proteins (OPR), encoded in the nucleus, control the expression of a specific organellar mRNA. The OPR repeat is a degenerate motif of 38 amino-acids, folding into a tandem of antiparallel α-helices which can bind to RNA. An individual OPR repeat is predicted to interact with one given nucleotide thanks to specificity-conferring residues at defined positions within the repeat. OPR proteins contain tracks of successive OPR motifs, thus they can bind to a specific RNA “target” sequence and act on it. I aimed to study this specificity, called the “OPR code”, starting with a draft code based on known OPR protein/mRNA couples. I mutated in vivo the chloroplast targets of some OPR factors to disrupt the OPR/RNA interaction, and then tried to restore it by mutating the specificity-conferring residues in the corresponding repeats. Surprisingly, OPR/RNA interactions seem very resilient, challenging our view of how the specificity is established in vivo. Complementary functional studies that I performed on the OPR factors MDB1 and MTHI1 revealed that chloroplast gene expression might rely on complex networks of nuclear factors. By cooperating those putative systems would be both more specific and more resilient
Книги з теми "MRNA report"
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Walsh, Richard A. Parkinson’s Disease or Essential Tremor? Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780190607555.003.0016.
Повний текст джерелаАнотація:
Fragile X-associated tremor ataxia syndrome is a heredodegenerative syndrome that presents in older men as a tremor syndrome with less prominent ataxia and cognitive impairment initially. The underlying genetic cause, a premutation in the FMR1 gene, results in a toxic accumulation of mRNA. The full mutation, a triple-repeat expansion of more than 200 CGG repeats, gives rise to a reduction in FMR1 protein expression and fragile X, a neurodevelopmental disorder that may be identified in successive male generations. The prevalence of carrier status is high in the general population, and it is likely that most movement disorders clinics will have one or more patients with this syndrome, potentially carrying a label of essential tremor.
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Abo-Shehada, Mahmoud N. The Myiases. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0075.
Повний текст джерелаАнотація:
Human myiases can be caused by over 50 species of dipteran larvae. The numbers of human clinical myiasis reports, reflect their relative importance in the following order; cutaneous, ophthalmomyiases, nasal, oral, intestinal, ear, urogenital, and cerebral myiases. Myiasis producing flies are distributed worldwide, but most reported cases are from warm and developing countries. Molecular techniques have been applied to myiasis fly identification and classification, especially ostrids and calliphorines. Successful elimination programs have been carried out against Hypoderma spp. in the UK and Cochliomyia hominivorax in the USA, Mexico, Central America, Libya and the Caribbean Islands and another is ongoing against Crysomya bezziana in the Middle East. A beneficial myissis “Biosurgery or maggot therapy” is the intentional use of Lucilia sericata larvae applied in specially designed dressings to chronic and MRSA infected wounds. The growing larvae execration/secretion facilitate wound debridement and successfully treated leg and pressure ulcers, wounds associated with diabetes, and many other types of infected wounds in a shorter time compared to conventional treatment. Now knowledge of myiases producing flies is accepted in many countries as a forensic tool.
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Vermeulen, Roel, Douglas A. Bell, Dean P. Jones, Montserrat Garcia-Closas, Avrum Spira, Teresa W. Wang, Martyn T. Smith, Qing Lan, and Nathaniel Rothman. Application of Biomarkers in Cancer Epidemiology. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780190238667.003.0006.
Повний текст джерелаАнотація:
Advancements in OMICs are now enabling investigators to explore comprehensively the biological consequences of exogenous and endogenous exposures by detecting molecular signatures of exposure, early signs of adverse biological effects, preclinical disease, and molecularly defined cancer subtypes. These new technologies have proven invaluable for assembling a comprehensive portrait of human exposure, health, and disease. This includes hypothesis-driven biomarkers, as well as platforms that can agnostically analyze entire biologic processes and “compartments,” including the measurement of small molecules (metabolomics), DNA polymorphisms and rarer inherited variants (genomics), methylation and microRNA (epigenomics), chromosome-wide alterations, mRNA (transcriptomics), proteins (proteomics), and the microbiome (microbiomics). Although the implementation of these technologies in epidemiologic studies has already shown great promise, some challenges of particular importance must be addressed. Non-genetic OMIC markers vary over time due to both random variation and physiologic changes. Therefore, there is an urgent need for cohorts to collect repeat biological samples over time.
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Jane, Buttimer, and Ireland. Department of Health., eds. Control of methicillin resistant staphylococcus aureus [MRSA] in the Irish health care setting: Report of a committee established by the Department of Health. [Dublin]: Department of Health, 1995.
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McGregor, Laura, Monica N. Gupta, and Max Field. Septic arthritis in adults. Oxford University Press, 2013. http://dx.doi.org/10.1093/med/9780199642489.003.0098.
Повний текст джерелаАнотація:
Septic arthritis (SA) is a medical emergency with mortality of around 15%. Presentation is usually monoarticular but in more than 10% SA affects two or more joints. Symptoms include rapid-onset joint inflammation with systemic inflammatory responses but fever and leucocytosis may be absent at presentation. Treatment according to British Society of Rheumatology/British Orthopaedic Association (BSR/BOA) guidelines should be commenced if there is a suspicion of SA. At-risk patients include those with primary joint disease, previous SA, recent intra-articular surgery, exogenous sources of infection (leg ulceration, respiratory and urinary tract), and immunosupression because of medical disorders, intravenous drug use or therapy including tumour necrosis factor (TNF) inhibitors. Synovial fluid should be examined for organisms and crystals with repeat aspiration as required. Most SA results from haematogenous spread-sources of infection should be sought and blood and appropriate cultures taken prior to antibiotic treatment. Causative organisms include staphylococcus (including meticillin-resistant Staphylococcus aureus, MRSA), streptococcus, and Gram-negative organisms (in elderly patients), but no organism is identified in 43%, often after antibiotic use before diagnosis. Antibiotics should be prescribed according to local protocols, but BSR/BOA guidelines suggest initial intravenous and subsequent oral therapy. Medical treatment may be as effective as surgical in uncomplicated native SA, and can be cost-effective, but orthopaedic advice should be sought if necessary and always in cases of infected joint prostheses. In addition to high mortality, around 40% of survivors following SA develop limitation of joint function. Guidelines provide physicians with treatment advice aiming to limit mortality and morbidity and assist future research.
Частини книг з теми "MRNA report"
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Shen, Xiaojuan, Yi Zhang, and Guanglin Li. "A Short Report on the Relation between the mRNA Sequence and the Encoded Protein Structure." In IFMBE Proceedings, 130–31. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-03005-0_33.
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Geissler, Rene, and Andrew Grimson. "Characterizing mRNA Sequence Motifs in the 3′-UTR Using GFP Reporter Constructs." In mRNA Decay, 77–88. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7540-2_6.
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Markina, Nadezhda M., Anton P. Pereverzev, Dmitry B. Staroverov, Konstantin A. Lukyanov, and Nadya G. Gurskaya. "Generation of Cell Lines Stably Expressing a Fluorescent Reporter of Nonsense-Mediated mRNA Decay Activity." In mRNA Decay, 187–204. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7540-2_14.
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Nickless, Andrew, and Zhongsheng You. "Studying Nonsense-Mediated mRNA Decay in Mammalian Cells Using a Multicolored Bioluminescence-Based Reporter System." In mRNA Decay, 213–24. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7540-2_16.
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Zhang, Zhaiyi, Amit Khanna, and Stefan Stamm. "Fast Cloning of Splicing Reporter Minigenes." In Alternative pre-mRNA Splicing, 381–91. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2012. http://dx.doi.org/10.1002/9783527636778.ch35.
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Higuchi, Takashi, Masahisa Kobayashi, Jin Ogata, Eiko Kaneshiro, Yohta Shimada, Hiroshi Kobayashi, Yoshikatsu Eto, et al. "Identification of Cryptic Novel α-Galactosidase A Gene Mutations: Abnormal mRNA Splicing and Large Deletions." In JIMD Reports, 63–72. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/8904_2015_475.
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Roberts, Rhonda S., Boyd L. Yount, Amy C. Sims, Susan Baker, and Ralph S. Baric. "Renilla Luciferase as a Reporter to Assess SARS-CoV mRNA Transcription Regulation and Efficacy of ANTI-SARS-CoV Agents." In Advances in Experimental Medicine and Biology, 597–600. Boston, MA: Springer US, 2006. http://dx.doi.org/10.1007/978-0-387-33012-9_108.
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Jacobson, Marty R., and Thoru Pederson. "RNA Traffic and Localization Reported by Fluorescent Molecular Cytochemistry in Living Cells." In mRNA Formation and Function, 341–59. Elsevier, 1997. http://dx.doi.org/10.1016/b978-012587545-5/50019-7.
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Armstrong, Tyler D., Usa Suwannasual, Conner L. Kennedy, Akshaykumar Thasma, Leah J. Schneider, Danielle Phillippi, and Amie K. Lund. "Exposure to Traffic-Generated Pollutants Exacerbates the Expression of Factors Associated with the Pathophysiology of Alzheimer’s Disease in Aged C57BL/6 Wild-Type Mice." In Advances in Alzheimer’s Disease. IOS Press, 2021. http://dx.doi.org/10.3233/aiad210017.
Повний текст джерелаАнотація:
Background: Multiple studies report a strong correlation between traffic-generated air pollution-exposure and detrimental outcomes in the central nervous system (CNS), including Alzheimer’s disease (AD). Incidence of AD is rapidly increasing and, worldwide, many live in regions where pollutants exceed regulatory standards. Thus, it is imperative to identify environmental pollutants that contribute to AD, and the mechanisms involved. Objective: We investigated the effects of mixed gasoline and diesel engine emissions (MVE) on the expression of factors involved in progression of AD in the hippocampus and cerebrum in a young versus aged mouse model. Methods: Young (2 months old) and aged (18 months old) male C57BL/6 mice were exposed to either MVE (300 μg/m3 PM) or filtered air (FA) for 6 h/d, 7 d/wk, for 50 d. Immunofluorescence and RT-qPCR were used to quantify oxidative stress (8-OHdG) and expression of amyloid-β protein precursor (AβPP), β secretase (BACE1), amyloid-β (Aβ), aryl hydrocarbon receptor (AhR), cytochrome P450 (CYP) 1B1, angiotensin-converting enzyme (ACE1), and angiotensin II type 1 (AT1) receptor in the cerebrum and hippocampus, in addition to cerebral microvascular tight junction (TJ) protein expression. Results: We observed age-related increases in oxidative stress, AhR, CYP1B1, Aβ, BACE1, and AT1 receptor in the CA1 region of the hippocampus, and elevation of cerebral AβPP, AhR, and CYP1B1 mRNA, associated with decreased cerebral microvascular TJ protein claudin-5. MVE-exposure resulted in further promotion of oxidative stress, and significant increases in AhR, CYP1B1, BACE1, ACE1, and Aβ, compared to the young and aged FA-exposed mice. Conclusion: Such findings suggest that MVE-exposure exacerbates the expression of factors in the CNS associated with AD pathogenesis in aged populations.
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La Serna, Miguel. "Gone in 90 Seconds." In With Masses and Arms, 29–38. University of North Carolina Press, 2020. http://dx.doi.org/10.5149/northcarolina/9781469655970.003.0003.
Повний текст джерелаАнотація:
This MRTA’s early armed actions in 1984 involve symbolic gestures, as the rebels seek to appropriate names like Tupac Amaru and Micaela Bastidas, places like Cuzco, and other sights of national historical memory. These actions culminate in the kidnapping of “90 Seconds” reporter Vicky Pelaez, in which MRTA leaders Nestor Cerpa and Victor Polay play a direct role.
Тези доповідей конференцій з теми "MRNA report"
1
Sun, Jiashu. "Inertial Microfluidics for Separation and Detection of Tumor Cells." In ASME 2013 4th International Conference on Micro/Nanoscale Heat and Mass Transfer. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/mnhmt2013-22036.
Повний текст джерелаАнотація:
We report on the development of a curved microfluidic channel that allows rapid and continuous size-based rare tumor cell separation from blood in a label-free manner by exploiting the hydrodynamic effects. The separated tumor cells are trapped and enriched on an integrated polycarbonate filter glued on top of the outlet reservoir of microchannels. CK19 mRNA of MCF-7 cells are detected by loop-mediated isothermal amplification (LAMP).
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Rossi, Joanna, Léonie Rouleau, Jean-Claude Tardif, and Richard L. Leask. "Fluid Shear Stress Reduces Simvastatin Induced Adhesion Molecule Expression in Cytokine Activated Endothelial Cells." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206539.
Повний текст джерелаАнотація:
Although originally designed as inhibitors of cholesterol biosynthesis, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, or statins, are now known to also have non-lipid lowering benefits [1]. Statins have been reported to modulate gene expression in endothelial cells, however, the effect of statins on adhesion molecule expression is contradictory. Some studies report a decrease in adhesion molecule mRNA and/or protein after statin treatment [2], while others have shown that statins potentiate the effect of tumor necrosis factor alpha (TNFα) [3]. To the best of our knowledge, the effects of statins on gene expression in cultured endothelial cells has been done in static conditions only and no study has examined the effect of blood flow. This is particularly important since fluid shear stress is a strong regulator of endothelial cell function and phenotype [4]. The purpose of this study was to clarify the effects of statins on vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) expression in endothelial cells by evaluating their biological response under fluid flow.
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Marquerie, G., A. Duperray, G. Uzan, and R. Berthier. "BIOSYNTHETIC PATHWAYS OF THE PLATELET FIBRINOGEN RECEPTOR IN HUMAN MEGAKARYOCYTES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642954.
Повний текст джерелаАнотація:
Interaction between cells and between cells and extracellular matrices are critical for a number of biological processes, including organ development, cell differenciation, cell motility, and the inimune' response. These interactions are mediated by a family of adhesion receptors that recognize short sequences such as Arg-Gly-Asp (RGD). These receptors share similar structural properties. They are heterodimers composed of a and B subunits and sometime express common epitopes. This suggests that the structural and functional relationship of these receptors may result from the transcription of related genes or may arise from cell specific post-transcriptional events. Thus, analysis of the biosynthesis and processing of these receptors would provide valuable insights into the molecular mechanism which control their expression at the surface,of different cells. Platelet membrane glycoprotein (GP) IIb-IIIa is a member of this receptor family. This protein is a non covalent heterodimer composed of two distinct polypeptides, Glib which consists of two subunits Ilba and IlbB (Mr = 116 kD, Mr = 25 kD) and GPIIIa (Mr = 100 kD, reduced). GPIIb-IIIa functions at site of platelet aggregation and serves as receptor for RGD containing factors including fibrinogen, fibronectin and von Willebrand factor. We report here on the investigation of the biosynthetic pathways of this RGD receptor in human megakaryocytes. High number of megakaryocytic cells from the megakaryoblastic stage to the polyploid mature megakaryocyte were obtained from liquid culture of cryopreserved leukocyte stem cell concentrates from patients with chronic myelogenous leukemia (CML). After sorting, using a FACS IV and indirect immunofluores-cent labeling with monoclonal antibodies anti-GPIIb-IIIa, 95 % of the cells in culture were of the megakaryocytic lineage. These megakarocytes represented an excellent tool to delineate at the molecular level events associated with the biosynthesis of GPIIb-IIIa.Metabolic labeling and pulse-chase experiments indicated that GPIIb and GPIIIa are synthesized from separate mRNA and that the two subunits of GPIIb derive from a common precursor. This was further confirmed by cell-free translation of megakaryocyte mRNA and the identification of separate cDNA containing sequences coding for the pro-GPIIb and for GPIIIa. These cDNA were isolated from a Xgt11 expression library constructed with purified megakaryocyte RNA, and were used to size the messengers coding for the two polypeptides. A single mRNA species of 3.9 kB was found to encode the pro-GPIIb, whereas two different mRNA species of 2.9 kB and 4. 1 kB were identified with the GPIIIa cDNA.The newly synthesized GPIIIa associates early with the pro-GPIIb in the rough endoplasmic reticulum. Examination of the glycosylation pathways with endoglycosidase H, tunicamycin and monensin indicated that high mannose oligosaccharides are added to the GPIIIa and pro-GPIIb polypeptide backbone. The pro-GPIIb is then processed with conversion of high mannose to the complex type carbohydrate, whereas GPIIIa remains endoH sensitive. Glycosylation of pro-GPIIb-IIIa and processing of oligosaccharides are prerequisite for proteolytic maturation of pro-GPIIb and the expression of the mature complex at the surface of the cell. Thus post-translational processing of GPIIb-IIIa requires an early assembly of the complex. This may have important implications in the maturation of megakaryocyte granules and in the molecular mechanism underlying the Glanzmann thrombastenic disease.
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Sato, Katsuya, and Toshihiko Shiraishi. "Measurement of Modes of Vibration of a Cultured Cell for its Mechanosensing Mechanisms." In ASME 2020 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/imece2020-23697.
Повний текст джерелаАнотація:
Abstract Applying mechanical vibration to cultured cells gives intracellular biochemical responses activated, for instance, their gene expression level is increased, so that it can be applied in the medical field. However, the cellular mechanisms of sensing mechanical vibration and transducing into the biochemical responses have not been clarified. One of the previous studies culturing osteoblastic cells under mechanical vibration showed that some of the intracellular biochemical responses reached peak depending on the frequencies like a resonance in the mechanical engineering field; the mRNA level of alkaline phosphatase at the frequency of 50 Hz reaches approximately 4.5 times as high as that at the control group [1]. Considering the analogy between the mechanical and the biochemical responses of a cell, the modes of vibration of a cell are thought to be related to the mechanosensing. In this study, the mode shapes of a single cell were experimentally measured under mechanical vibration up to 100 Hz using an improved experimental system with high natural frequencies designed to be low mass and high rigidity. This present paper will report the obtained experimental results of the mode shapes of a single cell nucleus and may contribute to the elucidation of the mechanosensing mechanisms of a cell for mechanical vibration.
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van den Berg, E. A., E. Sprengers, M. Jaye, W. Burgess, and V. W. M. van Hinsbergh. "REGULATION OF PLASMINOGEN ACTIVATOR INHIBITOR-1 mRNA IN HUMAN ENDOTHELIAL CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642856.
Повний текст джерелаАнотація:
Cultured human endothelial cells (HEC) increase their production of plasminogen activator inhibitor (PAI-1) upon stimulation with endotoxin and IL-1, agents that are known to cause an increase in PAI-1 levels in vivo. In order to study the regulation of PAI-1 synthesis at the mRNA level, we isolated a cDNA clone for the human PAI-1 gene from an endothelial expression cDNA library in λ gt 11 by screening with a PAI-1 specific antibody. Three positive cross-hybridizing clones were isolated. The longest insert (1500 bp) was partially sequenced (1000 bp). The sequence was identical to the PAI-1 sequence recently reported by others. The identity of the cDNA clone was further confirmed by comparison with part of the amino acid sequence of PAI-1. For that purpose t-PA-PAI-1 complex was purified from HEC conditioned medium by immunoadsorption to anti-t-PA IgG, and a suitable peptide was sequenced after comparison of the HPLC elution profiles of CNBr digests of t-PA and t-PA-PAI-1 complex. The amino acid sequence (M)FRQFQADFT completely matches the sequence predicted from the cDNA sequence.By hybridization of the cDNA probe to Northern blots of total cellular RNA from human umbilical vein and artery EC (HUVEC, HUAEC), two transcripts of 2.3 and 3 kb were found. Primary HUAEC, incubated for 18 hours in growth medium, produced considerable although variable levels of PAI-1 activity and contained PAI-1 mRNA levels comparable to those found in subcultured HUAEC. When subcultured HUEC were incubated for 6 h with endotoxin, IL-1 or TNF, a 2-fold increase in PAI-1 mRNA was found with each of these mediators. Stimulation of the cells in the presence of cycloheximide resulted in a further increase of the 3 kb PAI-1 transcript. The 3’ end of this transcript contains a 75 bp AT-rich sequence. Similar 3’ AT-rich sequences have been found in mRNA’s for a number of inflammatory mediators and cellular oncogenes, and in some cases it has been shown that removal of the sequence increased mRNA stability. The influence of cyclohex-imid on the larger PAI-1 transcript might be explained by inhibition of synthesis of a specific nuclease that controls the level of mRNA’s harbouring such an AT rich sequence.
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O'hara, Patrick J., Frank A. Grant, A. Betty, J. Haldmen, and Mark J. Murray. "Structure of the Human Factor VII Gene." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643786.
Повний текст джерелаАнотація:
Factor VII is a member of a family of vitamin K-dependent, gamma-carboxylated plasma protein which includes factor IX, factor X, protein C, protein S and prothrombin. Activated factor VII (factor Vila) is a plasma serine protease which participates in a cascade of reactions leading to the coagulation of blood. Two overlapping genomic clones containing sequences encoding human factor VII were isolated and characterized. The complete sequence of the gene was determined and found to span 12.8 kilobases. The mRNA for factor VII as demonstrated by cDNA cloning is polyadenylated at multiple sites but contains only one AAUAAA poly-A signal sequence. The mRNA can undergo alternative splicing forming one transcript containing eight segments as exons and another with an additional exon which encodes a larger pre-pro leader sequence. The portion of the pre-pro leader coded for by the additional exon has no known counterpart in the other vitamin K-dependent proteins. The positions of the introns with respect to the amino acid sequence encoded by the eight essential exons of factor VII are the same as those present in factor IX, factor X, protein C and the first three exons of prothrombin. These exons code for domains generally conserved among members of this gene family, including a pre-pro leader (the essential exon la and alternative exon lb), a gamma-carboxylated domain (exons 2 and 3) a growth factor domain (exons 4 and 5) an activation region (exon 6) and a serine protease (exon 8). The corresponding introns in these genes are dissimilar with respect to size and sequence, with the exception of the third intron in factor VII and protein C. Four introns and a portion of exon 8 in factor VII contain regions made up of tandem repeats of oligonucleotide monomer elements. More than a quarter of the intron sequences and more than a third of the 3' untranslated portion of the mRNA transcript consist of these minisatellite tandem repeats. This type of structure is responsible for polymorphisms due to allelic variation in repeat copy number in other areas of the human genome. Tandem repeats can evolve as a result of random crossover in DNA whose sequence is not maintained by selection. This suggests that much of the sequence information present in the introns and untranslated portion of the message is dispensable.
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Holliday, Casey J., Randall F. Ankeny, Hanjoong Jo, and Robert M. Nerem. "Discovery of Side- and Shear-Dependent miRNAs and mRNAs in Human Aortic Valvular Endothelial Cells." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53315.
Повний текст джерелаАнотація:
Aortic valve (AV) disease is diagnosed by severe symptoms, such as calcification, and typically treated by AV replacement and repair surgeries. The mechanism by which AV disease occurs, specifically the role of the endothelium remains relatively unknown. It is known that disease preferentially occurs on the fibrosa, or aortic side, where it is exposed to disturbed, oscillatory flow, whereas the ventricularis, or side facing the left ventricle, experiences pulsatile, laminar shear and remains non-calcified [1, 2]. Research shows that regulation of miRNAs, short nucleotide segments targeting mRNAs, coincides with cardiovascular pathologies [3] though expression profiles of miRNAs and the mRNAs they modulate in human AV endothelial cells (HAVECs) have not been reported. We hypothesize that disturbed flow conditions present on the fibrosa stimulate ECs to modify expression of genes and miRNAs to induce a pro-inflammatory phenotype.
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Leung, Lai Yee, Pamela J. VandeVord, Warren Hardy, Roche De Guzman, King H. Yang, and Albert I. King. "Effects of Short Duration Overpressure on Astrocytes: An In Vitro Study." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176202.
Повний текст джерелаАнотація:
Blast wave overpressure from detonations can injure physiological systems ‘silently.’ Experimental and clinical studies have revealed the damaging effects of shock waves on different physiological systems, such as ears, lungs and gastrointestinal tracts [1, 2]. Despite the improved helmet and body armor, many veterans returning from wars suffered from neurological disorders that are being diagnosed as mild traumatic brain injury. Warden (2006) reported that most of these veterans were exposed to blast [3]. In vivo study illustrated neuronal degeneration in the brain after exposure to blast waves [4]. As with many neuronal diseases, blast-induced neuronal injury may be related to microglia and astrocyte activation. However, the underlying mechanism is not clearly understood. This study was aimed at investigating the effects of short duration overpressure on astrocytes, in terms of cell proliferation and mRNA expression of several apoptotic genes and glial fibrillary acidic protein (GFAP).
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Fiszer, Agnieszka, Łukasz Przybył, Magdalena Jazurek-Ciesiołka, Adam Ciesiołka, Paweł Joachimiak, Emilia Kozłowska, Michał Michalak, et al. "I03 Mutant HTT MRNA as therapeutic target in allele-selective cag repeat-directed rnai approach and putative pathogenic agent in hd." In EHDN 2018 Plenary Meeting, Vienna, Austria, Programme and Abstracts. BMJ Publishing Group Ltd, 2018. http://dx.doi.org/10.1136/jnnp-2018-ehdn.239.
Повний текст джерела
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Higuchi, Makoto, Katsumi Sakaguchi, and Yuichiro Nomura. "Effects of Strain Holding and Continuously Changing Strain Rate on Fatigue Life Reduction of Structural Materials in Simulated LWR Water." In ASME 2007 Pressure Vessels and Piping Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/pvp2007-26101.
Повний текст джерелаАнотація:
The fatigue life reduces remarkably with reduction in strain rate in simulated light water reactor (LWR) water but the effects of strain wave form on this reduction are still not clear. This paper provides fatigue life data obtained from stepwise strain rate change tests, sine wave tests and strain holding tests. The effects of varying strain rate on fatigue life reduction can be estimated very well by the modified rate approach (MRA) method in the case of the step wise strain rate changing as shown in authors’ previous papers [1, 2, 3, 4, 5]. In the case of sine wave, however, the fatigue life reduction is much less compared to that predicted by the MRA method. The mechanism of such difference is not clear and the quantitative assessment of the fatigue life reduction caused by irregular strain wave form in actual transient seems impossible. The current MRA method gives always conservative assessment for sine wave straining and thus it is judged that this method need not be revised any more. The fatigue life reduction caused by strain holding at the peak of straining cycle in simulated BWR water had been reported in the previous paper [6]. In actual thermal transients, however, strain is not usually held at the peak of straining cycle but at the point somewhat reduced from the peak after the stabilization of temperature. In considering this phenomenon, additional fatigue tests in which the strain was held at the point somewhat reduced from the peak were carried out. In such conditions, the fatigue life reduction caused by strain holding disappeared. The similar fatigue tests with peak strain holding were also carried out in simulated PWR water and no fatigue life reduction can be observed. Considering the effects of strain holding on fatigue, the model for evaluating fatigue life reduction in LWR water was revised.
Звіти організацій з теми "MRNA report"
1
Stern, D. B. Differential regulation of plastid mRNA stability. Progress report. Office of Scientific and Technical Information (OSTI), September 1993. http://dx.doi.org/10.2172/10175480.
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von Arnim, Albrecht G. Eukaryotic initiation factor 3 (eIF3) and 5’ mRNA leader sequences as agents of translational regulation in Arabidopsis. Final report. Office of Scientific and Technical Information (OSTI), February 2015. http://dx.doi.org/10.2172/1169186.
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Maquat, Lynne. Final report: FASEB Summer Research Conference on ''Post-transcriptional control of gene expression: Effectors of mRNA decay'' [agenda and attendees list]. Office of Scientific and Technical Information (OSTI), December 2002. http://dx.doi.org/10.2172/808649.
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Talukder, Md, Ubaidur Rob, Laila Rahman, A. K. M. Zafar Khan, Riad Mahmud, Azizul Alim, Ismat Hena, Farhana Akter, and Anup Dey. Incentivizing providers to improve maternal, newborn and child health services in Bangladesh: Pay-for-performance model refinement and advocacy (P4P MRA) final report. Population Council, 2012. http://dx.doi.org/10.31899/rh2.1022.
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Health hazard evaluation report: HETA-2009-0098-3103, evaluation of methicillin-resistant Staphylococcus aureus (MRSA) cases among employees at a workholding manufacturing facility, Positrol Inc., Cincinnati, Ohio. U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, March 2010. http://dx.doi.org/10.26616/nioshheta200900983103.
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