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Добірка наукової літератури з теми "Mycorrhizal fungi"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 9 червня 2025

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Статті в журналах з теми "Mycorrhizal fungi"

Valdes, María. "Aspectos ecofisiológicos de las micorrizas." Botanical Sciences, no. 49 (April 10, 2017): 19. http://dx.doi.org/10.17129/botsci.1363.

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Mycorrhiza is the part of the roots infected with particular soil fungi. This type of association is formed by most of the plants. There are several types of mycorrhizae; this short review is concerned only with Ectomycorrhiza (EM) and the Vesicular-Arbuscular Mycorrhiza (VAM). These two types are the most common in nature. EM has a compact fungus mantle over the root surface and intercellular hypha in the cortex; the V AM has a loose network of hyphae in the soil surrounding the root and hyphal growth within the cortical cells. Mycorrhizas increase nutrient uptake and hence plant growth. Since mycorrhizas are surrounded by an extensive hyphal network than may extcnd into the soil, this network represents a greater surface area, in other words, mycorrhizas shorten the distance that nutrients must diffuse through the soil to the root and their hyphae increase the volume of soil available to the plant for nutrient uptake. Physiological responses to root colonization with mycorrhizal fungi by most of the plants are dependent on the level of soil fertility and on the degree of mycorrhizal dependency of the plant. Soils having a high fertility have mostly a poor colonization, hence, for plant growth to respond to inoculation, soils must have a low fertility. Mycorrhizal dependency can be very different among plant species; plants with short root hairs are more dependent on mycorrhizal fungi. Most soils contain mycorrhizal fungi and their distribution varies with climatic, edaphic environment and land use. There are differences in effectiveness in colonization and in enhanced nutrient uptake among the fungi.

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Bryndina, Larisa, Yuliya Arnaut, and Olesya Alykova. "MYCORRHIZAL FUNGI IN THE FORMATION OF BIOGEOCENOSES: ANALYTICAL REVIEW." Forestry Engineering Journal 12, no. 1 (April 15, 2022): 5–24. http://dx.doi.org/10.34220/issn.2222-7962/2022.1/1.

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This review examines the results of scientific experiments of foreign and domestic researchers in the field of studying mycorrhizal associations, the mechanisms of their symbiosis with plants. The information about the current state of the issue is given: different points of view on the interaction of mycorrhizal plants with mycobionts. A comparative analysis of the development of mycorrhiza in individual plant species was carried out. It was found that the maximum degree of mycorrhiza development corresponds to the beech and linden families. The factors influencing the development of balanced and exploitive mycorrhizal associations are considered. The evolutionary and functional characteristics of the types of mycorrhizae are given. The benefits of mycorrhizal associations are considered, both for tree species and for species of fungi involved in the formation of mycorrhizae. The role of symbionts in mycorrhiza is considered, as well as the form and degree of development of mycorrhiza for mycotrophic plants. An analytical review of the studies of foreign and domestic scientists allowed us to determine the preferred mycorrhizal communities for reforestation. It was noted that unfavorable, extreme environmental conditions in most cases intensified the growth and development of mycorrhizal communities. All these factors should be taken into account when selecting tree species and types of fungi in forestry during reforestation

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Ramakrishnan, K., and G. Bhuvaneswari. "Influence on Different Types of Mycorrhizal Fungi on Crop Productivity in Ecosystem." International Letters of Natural Sciences 38 (May 2015): 9–15. http://dx.doi.org/10.18052/www.scipress.com/ilns.38.9.

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Mycorrhizal fungi greatly enhanced the ability of plants to take up phosphorus and other nutrients those are relatively immobile and exist in low concentration in the soil solution. Fungi can be important in the uptake of other nutrients by the host plant. Mycorrhizae establish symbiotic relationships with plants and play an essential role in plant growth, disease protection, and overall soil quality. Of the seven types of mycorrhizae described in current scientific literature (arbuscular, ecto, ectendo, arbutoid, monotropoid, ericoid and orchidaceous mycorrhizae), the arbuscular and ectomycorrhizae are the most abundant and widespread. This chapter presents an overview of current knowledge of mycorrhizal interactions, processes, and potential benefits to society. The molecular basis of nutrient exchange between arbuscular mycorrhizal (AM) fungi and host plants is presented; the role of AM fungi in disease protection, alleviation of heavy metal stress and increasing grain production. Most land plants form associations with mycorrhizal fungi. Mycorrhizas are mutualistic associations between fungi and plant roots. They are described as symbiotic because the fungus receives photo synthetically derived carbon compounds and the plant has increased access to mineral nutrients and sometimes water.

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Ramakrishnan, K., and G. Bhuvaneswari. "Influence on Different Types of Mycorrhizal Fungi on Crop Productivity in Ecosystem." International Letters of Natural Sciences 38 (May 6, 2015): 9–15. http://dx.doi.org/10.56431/p-9pjdc8.

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Dalpé, Y. "Mycorrhizal fungi biodiversity in Canadian soils." Canadian Journal of Soil Science 83, Special Issue (August 1, 2003): 321–30. http://dx.doi.org/10.4141/s01-067.

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The term "mycorrhiza" refers to the mutualistic symbiosis between fungi and the roots of a vast majority of vascular plants, distributed over almost any ecosystem. The fungal symbionts, primarily recognized for their beneficial impact on plant growth and plant protection, are integral components of soil ecosystems and as such, play an active role in improving plant productivity and diversity, soil microflora and microfauna diversity, and soil quality. Classified among major groups of true fungi, the study of their biodiversity and distribution has been explored for only a few decades. Major emphasis was first put on the mycorrhizal status of plants, on the evaluation of their benefits to plant growth, on their geographic distribution and soil inventories, and more recently on their relationship to plant diversity and productivity. In the 1950s, Canadian scientists were among world pioneers in the field of mycorrhizae research and they continue to be recognized by the international scientific community. Studies dealing with soil biodiversity of mycorrhizal fungi, including arbuscular, ecto-, ectendo-, ericoid- and orchid mycorrhizae, performed by Canadian researchers are presented here together with strategies and perspectives for a better exploitation of mycorrhizal fungal diversity in ecosystems. Key words: Symbiosis, mycorrhizae, arbuscular mycorrhizal fungi, ectomycorrhizal fungi, biodiversity, plant protection

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Taurinanda, Adventio Purnamadya, and Dina Rotua Valentina Banjarnahor. "Mycorrhiza Diversity in Some Intercropping Systems of Potato (Solanum tuberosum L) and Faba Bean (Vicia faba L)." Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) 12, no. 2 (June 15, 2023): 495. http://dx.doi.org/10.23960/jtep-l.v12i2.495-508.

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Arbuscular mycorrhizal fungi (AMF) is the most widely distributed mycorrhizal fungi in the soil and can make a symbiosis with the roots of host plants to form arbuscular mycorrhizal symbionts. Intercropping is a practice of polyculture cropping where two or more plant species are simultaneously cultivated in the same field. The objective of this study was to define the effect of intercropping on the density and diversity of mycorrhizal spores. In this study, potatoes and faba beans, both of which have the ability to symbiosis with mycorrhizae, were intercropped. A randomized group design with 5 planting system treatments was employed in this study with 5 replications. The results concluded that density of mycorrhizal spores in the intercropping planting pattern was not statistically different from the density of mycorrhiza in the monoculture cultivation pattern. The types of mycorrhiza found included the genus of Glomus, Funneliformis, Scutellospora, Cetraspora, Septoglomus, and Entrophospora Keywords: pH; Root exudate; Spore density, Spore identification; Spore diversity.

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Shamsudin, Nor Amirah, Jaya Seelan Sathiya Seelan, Jualang Azlan Gansau, and Nor Azizun Rusdi. "A review: Molecular identification of orchid mycorrhiza." Advances in Horticultural Science 38, no. 1 (April 4, 2024): 97–116. http://dx.doi.org/10.36253/ahsc-14952.

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Orchids are a diverse and widespread family of flowering plants, with over 25,000 known species and more than 100,000 hybrids and cultivars. Orchids are characterised by their often showy and highly specialised flowers and have unique and intricate floral. Orchids are known to be highly dependent on their mycorrhizal fungi for nutrient uptake, especially during the early stages of their development. Orchid seeds lack the endosperm present in most other seeds, which means they cannot germinate without a source of nutrition. The relationship between orchids and mycorrhiza is known as orchid mycorrhizae or orchid mycorrhiza. In orchid mycorrhiza, the orchid plant forms a mutualistic relationship with certain species of fungi that are able to penetrate the orchid’s roots and colonise its tissues to provides the orchid with essential nutrients. Orchid mycorrhizal fungi are often highly specific, meaning that they can only form partnerships with certain orchid species, and vice versa. The importance of mycorrhizal fungi in the orchid life cycle is crucial from both evolutionary and ecological standpoints. Therefore, it is essential to acquire a thorough comprehension of this relationship and develop methodologies for isolating, identifying, and preserving significant fungal strains that are associated with different orchid species. In recent years, there has been a considerable increase in research concentration on mycorrhizal interactions in orchids. However, certain inquiries remain unresolved pertaining to the fungal communities associated with orchids as well as the divergences notices across different species and geographical locales. The present paper provides a through, and extensive analysis of the fungal life associated with orchids. This article presents a succinct overview of the molecular techniques utilised by researchers globally to isolate and identify peloton-forming fungi in both temperate terrestrial and tropical orchids. The review begins by proving a concise introduction to the background material regarding the wide range of fungal species that are linked with orchids. It then proceeds to explores the topic of orchid mycorrhizal fungi (OMF) and orchid non-mycorrhizal fungi (ONF). The subsequent analysis explores the crucial function that orchid mycorrhizal fungi play in the processes of seed germination and development. Moreover, the study elaborates on the methodologies utilised for isolating fungi, extracting fungal DNA, selecting primers, amplifying DNA and subsequent analysis sequence data. This article considers several molecular identification approaches that are used in studying orchid endophytic mycorrhizal. Using molecular approaches, orchid mycorrhizal can be further explored and identified.

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Miller, R. M., B. A. D. Hetrick, and G. W. T. Wilson. "Mycorrhizal fungi affect root stele tissue in grasses." Canadian Journal of Botany 75, no. 10 (October 1, 1997): 1778–84. http://dx.doi.org/10.1139/b97-892.

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Although arbuscular mycorrhizal symbiosis was initially believed to have little or no impact on root morphology, we now recognize that subtle changes do occur and that these changes may be of considerable consequence to host growth and nutrition, as well as functional growth strategy. In examining the stele and root diameters of C3 and C4 grasses, C4 grasses were demonstrated to have a significantly larger proportion of their fibrous roots occupied by stele tissue than do C3 grasses. In fact, functional growth strategy (C3 versus C4) was observed to be a relatively good predictor of stele area. Mycorrhizal fungi also influenced the amount of stele tissue, but the effect was not the same for both C3 and C4 grasses. The stele area of all C4 grasses except for Sorghastrum nutans was greater in the presence of mycorrhizal colonization. Among the C3 grasses, only Bromus inermis showed a significant increase, although Elymus cinereus and Lolium perenne displayed significant decreases in response to arbuscular mycorrhizal colonization. Changes in the stele area of the plant species were closely related to their responsiveness to mycorrhizal symbiosis and might in part explain both beneficial and detrimental responses of plants to mycorrhizae. An increase in stele circumference induced by mycorrhizae would allow for greater uptake and passage of water and nutrients to the vascular cylinder, and growth depressions could be a direct outcome of reduced stele circumference. Thus, differences in stele circumference represent a possible mechanism for mycorrhizal impacts on host plants. These findings indicate that structural differences among grasses are related to different functional capabilities and further emphasize the need for better integration of comparative anatomy and morphology procedures in the study of mycorrhizal symbiosis. Key words: stele, root anatomy, mycorrhizal dependency, functional growth strategy, mycorrhiza, C3 and C4 grasses.

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Liu, Jin, Yang Xu, Yan-Ji Si, Bin-Qi Li, Peng Chen, Ling-Ling Wu, Pu Guo, and Rui-Qing Ji. "The Diverse Mycorrizal Morphology of Rhododendron dauricum, the Fungal Communities Structure and Dynamics from the Mycorrhizosphere." Journal of Fungi 10, no. 1 (January 14, 2024): 65. http://dx.doi.org/10.3390/jof10010065.

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It is generally believed that mycorrhiza is a microecosystem composed of mycorrhizal fungi, host plants and other microscopic organisms. The mycorrhiza of Rhododendron dauricum is more complex and the diverse morphology of our investigated results displays both typical ericoid mycorrhizal characteristics and ectomycorrhizal traits. The characteristics of ectendoomycorrhiza, where mycelial invade from the outside into the root cells, have also been observed. In order to further clarify the mycorrhizal fungi members and other fungal communities of R. dauricum mycorrhiza, and explore the effects of vegetation and soil biological factors on their community structure, we selected two woodlands in the northeast of China as samples—one is a mixed forest of R. dauricum and Quercus mongolica, and the other a mixed forest of R. dauricum, Q. mongolica, and Pinus densiflor. The sampling time was during the local growing season, from June to September. High-throughput sequencing yielded a total of 3020 fungal amplicon sequence variants (ASVs), which were based on sequencing of the internal transcribed spacer ribosomal RNA (ITS rRNA) via the Illumina NovaSeq platform. In the different habitats of R. dauricum, there are differences in the diversity of fungi obtained from mycorrhizal niches, and specifically the mycorrhizal fungal community structure in the complex vegetation of mixed forests, where R. dauricum is found, exhibits greater stability, with relatively minor changes over time. Soil fungi are identified as the primary source of fungi within the mycorrhizal niche, and the abundance of mycorrhizal fungi from mycorrhizal niches in R. dauricum is significantly influenced by soil pH, organic matter, and available nitrogen. The relationship between soil fungi and mycorrhizal fungi from mycorrhizal niches is simultaneously found to be intricate, while the genus Hydnellum emerges as a central genus among mycorrhizal fungi from mycorrhizal niches. However, there is currently a substantial gap in the foundational research of this genus, including the fact that mycorrhizal fungi from mycorrhizal niches have, compared to fungi present in the soil, proven to be more sensitive to changes in soil moisture.

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Perry, D. A., R. Molina, and M. P. Amaranthus. "Mycorrhizae, mycorrhizospheres, and reforestation: current knowledge and research needs." Canadian Journal of Forest Research 17, no. 8 (August 1, 1987): 929–40. http://dx.doi.org/10.1139/x87-145.

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Although not a panacea, management of mycorrhizae and associated organisms is an important reforestation aid. Its three major components are protection of the indigenous soil community and evaluation of inoculation needs, integration of inoculation programs into existing reforestation technology, and research. Clear-cutting frequently results in reduced mycorrhizae formation, particularly when reforestation is delayed and no other host plants are present to maintain fungal populations. Implications of such reductions for reforestation vary with environmental factors and tree species. Adequate mycorrhiza formation is especially critical for ectomycorrhizal trees growing on poor soils or in environments where seedlings must establish quickly to survive. It may also be important where early successional, noncrop plants do not support the same mycobiont as the crop. In such circumstances, a self-reinforcing trend may develop, with poor mycorrhiza formation reducing seedling survival and poor tree stocking leading to further loss of mycorrhizal inocula. Inoculating nursery seedlings with mycobionts holds promise for improving outplanting performance only if site-adapted fungi are used. A practical alternative is to improve nursery practices to enhance natural populations of mycorrhizal fungi. Seedlings leaving the nursery with diverse mycorrhizae may perform better than those leaving with only one or a few nursery-adapted types. Research is needed in three broad areas: on adaptations of mycorrhizal fungi to particular environmental factors; on interactions between tree seedlings and processes occurring within the sphere of influence of roots (the rhizosphere) or of mycorrhizal roots (the mycorrhizosphere); and on the role of mycorrhizae and associated organisms in ecosystem structure and processes, particularly nutrient cycling, plant-plant interaction, and soil structure.

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Більше джерел

Дисертації з теми "Mycorrhizal fungi"

Smith, S. E. "Studies on Mycorrhizal fungi." Title page, contents and abstract only, 1990. http://web4.library.adelaide.edu.au/theses/09SD/09sds659.pdf.

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Kasiamdari, Rina Sri. "Interactions between arbuscular mycorrhizal fungi and other root-infecting fungi." Title page, contents and abstract only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phk1887.pdf.

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Antoniolli, Zaida Inês. "Arbuscular mycorrhizal community in a permanent pasture and development of species-specific primers for detection and quantification of two AM fungi /." Title page, contents and summary only, 1999. http://web4.library.adelaide.edu.au/theses/09PH/09pha635.pdf.

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Stockinger, Herbert. "DNA barcoding of arbuscular mycorrhizal fungi." Diss., lmu, 2010. http://nbn-resolving.de/urn:nbn:de:bvb:19-114870.

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Rewcastle, Joanne. "Plant protection using arbuscular mycorrhizal fungi." Thesis, University of Edinburgh, 2005. http://hdl.handle.net/1842/27261.

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The interaction between several species of arbuscular mycorrhizal fungi, micropropagated strawberry plants and Phytophthora fragariae, the pathogen that causes red stele disease of strawberry plants, was investigated. The optimum temperature for germination of zoospore cysts of P. fragariae in vitro was found to be 15°C, and growth of the emerging germ tube was significantly orientated towards the strawberry root tip. Cyst germination was reduced in the presence of a mycorrhizal strawberry root. Elsanta was more susceptible to P. fragariae than the cultivar Rhapsody. A low level of colonisation of Elsanta with the arbuscular mycorrhizal fungi Glomus mosseae, Glomus intraradices or Glomus fistulosum resulted in a significantly greater amount of total phosphorus in plant shoots compared to non-mycorrhizal plants, although further increases in the percentage of root colonisation by the fungi had no effect on the plants. The presence of these mycorrhizal fungi had no effect on disease due to subsequent inoculation of the plants by P. fragariae. Increasing colonisation of Elsanta by Scutellospora nodosa was correlated with a significant increase in plant size and additional phosphorus uptake. However, these same plants exhibited greater levels of disease due to the following inoculation with P. fragariae. A low level of root colonisation of Elsanta by Acaulospora scrobiculata caused significant increases in plant size and phosphorus uptake up to a threshold level of root colonisation beyond which further increases had no affect on the plant. The results are discussed in relation to the utilisation of specific strains of arbuscular mycorrhizal fungi as inoculants of micropropagated strawberry plants of particular cultivars with the potential to increase plant growth and reduce the level of disease due to soil-borne plant pathogens.

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Monreal, Marcia Amelia. "Molecular identification of ericoid mycorrhizal fungi." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq25119.pdf.

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Stewart, Lynda Irene. "Phosphorus effects on arbuscular mycorrhizal fungi." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=102729.

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Two field studies were conducted to assess the potential benefit of arbuscular mycorrhizal (AM) inoculation of elite strawberry plants on plant multiplication, and fruit yield, under typical nursery conditions, in particular soils classified as excessively rich in P. To study plant productivity, five commercially in vitro propagated elite strawberry cultivars ('Chambly', 'Glooscap', 'Joliette', 'Kent', and 'Sweet Charlie') were not inoculated with AM fungi or were inoculated with either a single species (Glomus intraradices), or a mixture of species (G. intraradices, Glomus mosseae, and Glomus etunicatum). AM inoculation was found to impact strawberry plant productivity in a soil with excessive P levels. The AM fungi introduced into the field by inoculated mother plants established a mycelial network in the soil through colonization of the daughter plant roots, however, persistence of colonization was determined to below (<12% in inoculated plant roots). In soils excessively rich in P, individual crop inoculation may be the only option for management of the symbiosis, as the host and non-host rotation crops, planted prior to strawberry production, had no effect on plant productivity or soil mycorrhizal potential. To study the impact of AM inoculation on fruit production, three commercially grown strawberry cultivars (Glooscap, Joliette, and Kent) were not inoculated with AM fungi or were inoculated with either G. intraradices or G. mosseae. AM fungi impacted the fruit yield, with all inoculated cultivars producing more fruit than noninoculated cultivars during the first harvest year. The percentage of root colonization could not be used to explain the differences in total fruit yield during the first harvest year, or the increase in total fruit yield the second harvest year. We wished to examine the effects of various P treatments on C metabolism within the intraradical mycelia (IRM) of the fungus. Specific primers were developed for the Glomus intraradices glucose-6-phosphate dehydrogenase (G6PDH) gene. Real-time quantitative reverse transcriptase polymerase chain reaction (QRT-PCR) was used to measure the gene expression of the G. intrarardices G6PDH gene in response to external P conditions of colonized transformed carrot roots. The results showed a significant down-regulation of G6PDH in the IRM of G. intraradices when cultures were grown in a high P (350 muM P) medium compared to those grown in the low P (35 muM P) medium. The down-regulation may suggest a reduction in the C flow from the host to the fungus. There was no effect on G6PDH expression following a two-hour incubation with additional P applications (No P, low P and high P).

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Mathieu, Stephanie. "The Genetics of Arbuscular Mycorrhizal Fungi." Thesis, Université d'Ottawa / University of Ottawa, 2021. http://hdl.handle.net/10393/42770.

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Sexual reproduction is an important process amongst eukaryotic organisms, with one function being to maintain genetic variation. The idea that complex eukaryotic species can persist for millions of years in the absence of sex defies fundamental evolutionary dogma, yet a group of organisms known as ancient asexuals were thought to have evolved clonally under deep evolutionary time. Prominent among these are the arbuscular mycorrhizal fungi (AMF), which are obligate plant symbionts that colonize the root cells of plants and extend their hyphae into the soil assisting the plant in acquiring key nutrients. Unlike most eukaryotes, AMF cells are multinucleate with thousands of nuclei moving through a continuous cytoplasm. Genomic analyses have identified a putative mating-type (MAT) locus within the nuclear genomes of model AMF Rhizophagus irregularis, a region that in other fungi dictates the process of sexual reproduction. Additional findings demonstrated that AMF strains carry one of two nuclear organizations. They can be either homokaryotic (AMF homokaryons), where all nuclei within the cytoplasm are virtually identical, or heterokaryotic (AMF dikaryons), where two MAT-locus variants co-exist within the cytoplasm. Despite a lack of observable traits indicative of sex, this homo/heterokaryotic dichotomy is reminiscent of the nuclear organization of sexual fungi. My research aims to build on these findings to investigate the actual role of the MAT-locus in driving AMF reproduction. To address this, I build my thesis into three main chapters. The first chapter reviews our current understanding of AMF genetics and what drives genome evolution in these organisms. The second chapter establishes a relatively easy, inexpensive, and reproducible approach to genotype known MAT variants of R. irregularis in natural and experimental conditions. The last chapter uses experimental crossings between strains to assess cytoplasmic compatibility and nuclear exchange. I demonstrate that dikaryotic spore progenies can be formed after co-culturing two distinct AMF homokaryotic strains. Further analyses of various genomic regions also reveal possible recombination in homokaryotic spore progenies from co-cultures. Overall, this research provides new experimental insights into the origin of genetic diversity in AMF. These findings open avenues to produce genetically new AMF strains in the lab using conventional crossing procedures and provide a glimpse of the mechanisms that generate AMF genetic diversity in the field.

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Ike-Izundu, Nnenna Esther. "Interaction between arbuscular mycorrhizal fungi and soil microbial populations in the rhizosphere." Thesis, Rhodes University, 2008. http://hdl.handle.net/10962/d1004021.

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This study examined the rehabilitation potential of AM fungi with organic and inorganic fertilisers under pot and field trial conditions as well as their interaction with rhizospheric organisms and specific functional groups. In addition, the study highlighted the effects of land-use management on AM fungal populations in soil and the mycorrhizal status of some selected plants from one of the study sites. The study focussed on two sites that differ in operational activities and these included a mined area that was to be rehabilitated and a commercial farming site. A pot trial was conducted using an overburdened soil resulting from kaolin clay mining. Pots were seeded with Cynodon dactylon and treated with either Organic Tea or NPK (3:1:5) fertiliser, with or without AM fungal inoculum. The compatibility of these fertilisers with AM fungi was assessed by plant growth and percentage root colonisation. Maximum shoot height and plant biomass were observed at the 28th week with NPK (3:1:5) fertiliser supporting mycorrhizal colonisation by 80%. The result indicated the potential of AM fungi to be used in rehabilitation with minimal phosphate fertiliser. Similarly, a field trial was set-up using 17 x 17 m[superscript 2] plots in the mining site that were treated with the same organic and inorganic fertilisers as well as with AM fungal inoculum in different combinations. The interaction between AM fungi and soil microbial population was determined using culture dependent and culture independent techniques. The culture dependent technique involved the use of soil dilution and plating on general purpose and selective media. The result showed that there was no change in the total culturable bacterial number in the untreated and AM fungal treated plots, while a change in species composition was observed in the functional groups. Different functional groups identified included nitrogen fixing bacteria, pseudomonads, actinomycetes, phosphate solubilisers and the fungal counterparts. Gram-positive bacteria were observed as the predominant phenotypic type, while nitrogen fixers and actinomycetes were the predominant functional groups. Species identified from each functional group were Pseudomonas fulva, Bacillus megaterium, Streptomyces and actinomycetales bacteria. Meanwhile, fungi such as Ampelomyces, Fusarium, Penicillium, Aspergillus, Cephalosporium and Exserohilium were identified morphologically and molecularly. Furthermore, the mining site had a significantly higher bacterial number than the farming site thereby indicating the effects of land-use management on culturable bacterial numbers. The culture independent technique was carried out by cloning of the bacterial 16S rDNA and sequencing. Identified clones were Bradyrhizobium, Propionibacterium and Sporichthya. A cladogram constructed with the nucleotides sequences of identified functional species, clones and closely related nucleotide sequences from the Genbank indicated that nucleotide sequences differed in terms of the method used. The activity and establishment of the introduced AM fungal population was determined by spore enumeration, infectivity assay, percentage root colonisation and assessment of glomalin concentrations. The results indicated that the two land use types affected AM fungal populations. However, the establishment of AM fungi in the farming site was more successful than in the mining site as indicated by the higher infectivity pontential. Selected host plants, which were collected around the mine area, were observed to be mainly colonised by AM fungi and these were identified as Pentzia incana, Elytropappus rhinocerotis, Euphorbia meloformis, Selago corymbosa, Albuca canadensis and Helichrysum rosum. These plant species were able to thrive under harsh environmental conditions, thereby indicating their potential use as rehabilitation host plants. Generally, the findings of this study has provided an insight into the interaction between arbuscular mycorrhizal fungi and other soil microorganisms in two fields with differing land use management practices.

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Haller, Anjanette H. A. "The presence and role of arbuscular mycorrhizal fungi in coastal sand dune systems." Thesis, Rhodes University, 2000. http://hdl.handle.net/10962/d1003765.

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Arbuscular mycorrhizas (AM) are mutually beneficial symbiotic associations between the roots of plants and certain Zygomycetous fungi. The role of AM fungi in coastal sand dunes has been explored in many parts of the world, though little work has been conducted in South African dune systems. This study aimed to investigate the presence and extent of mycorrhizal colonisation of a coastal sand dune in South Africa. The roots of five plant species (Scaevola plumieri, Arctotheca populifolia, Ipomoea pes-caprae, Ehrharta villosa and Chrysanthemoides monilifera) were sampled along a foredune profile at Old Woman's River in the Eastern Cape. These roots were assessed for the percentage mycorrhizal colonisation they supported. Spores extracted from the rhizosphere sand of each plant species were counted and identified to genus level. Results were related to seasonality and the position of the plants along the profile. All plant species were found to be mycorrhizal. Percentage colonisation ranged from 0-92%, depending on plant species and season. Mycorrhizal colonisation was generally highest in the winter months, and especially so in I pes-caprae and E. villosa. The extent of various mycorrhizal structures in root tissue varied between plant species. Spore numbers ranged from 0-48 spores 100g-1 sand with highest numbers occurring in winter. S. plumieri and A. populifolia were associated with greatest spore abundance. Four fungal genera (Glomus, Acaulospora, Scutellospora and Gigaspora) were identified. Distribution of these genera showed seasonal variations between plant species. A bioassay, using Sorghum, was conducted to test the inoculum potentials of sand from the Scaevola hummock and the IpomoealEhrharta dune. Highest percentage colonisation occurred in plants grown in the Scaevola sand, which also had the lowest root and shoot measurements. The bioassay confirmed that AM propagules are present and viable, even in the mobile sand of the foredune. This study showed that mycorrhizal colonisation and spore numbers varied seasonally, but that the extent of this was dependent on plant species. The position of plants along the foredune profile tended to be less important than plant species. It is thought that the growth cycle and rooting system of each plant species determines seasonal cycles and abundance of AM fungi. Variation within fungal populations probably also impacts on this. Knowledge of the presence and distribution of AM fungi in this system paves the way for more detailed studies which need to examine the role of these endophytes in South African sand dunes.

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Книги з теми "Mycorrhizal fungi"

Ferrol, Nuria, and Luisa Lanfranco, eds. Arbuscular Mycorrhizal Fungi. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0603-2.

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Panneerselvam, Periyasamy, Pradeep Kumar Das Mohapatra, Amaresh Kumar Nayak, Kulandaivelu Velmourougane, Sergio De Los Santos-Villalobo, and Debasis Mitra. Arbuscular Mycorrhizal Fungi. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003354086.

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Fulton, Susanne M. Mycorrhizal fungi: Soil, agriculture, and environmental implications. New York: Nova Science Publishers, 2011.

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Agerer, R. Zur Ökologie der Mykorrhizapilze. Vaduz: J. Cramer, 1985.

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National Conference on Mycorrhiza (3rd 1995 New Delhi, India). Mycorrhizae, biofertilizers for the future: Proceedings of the Third National Conference on Mycorrhiza, 13-15 March 1995. Edited by Adholeya Alok, Singh Sujan, Tata Energy Research Institute, India. Dept. of Biotechnology., and India. Dept. of Science and Technology. New Delhi: Tata Energy Research Institute, 1995.

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O'Neill, Cathy. An evaluation of in vitro methods for the production of ectomycorrhizal fungus inoculum. Dublin: University College Dublin, 1995.

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J, Read D., C. A. B. International, and European Symposium on Mycorrhizae (3rd : 1991 : University of Sheffield), eds. Mycorrhizas in ecosystems. Wallingford, Oxon, UK: C.A.B International, 1992.

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Pagano, Marcela C., ed. Recent Advances on Mycorrhizal Fungi. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-24355-9.

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Souza, Tancredo. Handbook of Arbuscular Mycorrhizal Fungi. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-24850-9.

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Pagano, Marcela C., and Mónica A. Lugo, eds. Mycorrhizal Fungi in South America. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-15228-4.

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Частини книг з теми "Mycorrhizal fungi"

Pinhey, Sally, and Margaret Tebbs. "The role of fungi." In Plants for soil regeneration: an illustrated guide, 23–27. Wallingford: CABI, 2022. http://dx.doi.org/10.1079/9781789243604.0005.

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Abstract This chapter focuses on the role of fungi. Fungi are a vital part of the mixture of microorganisms found in healthy soil. Fungal associations between plant roots and beneficial fungi are known as mycorrhizae (meaning 'fungus' and 'root'), and form a beneficial or symbiotic relationship with plants growing in the soil. Mycorrhizal fungi also facilitate plant interactions with other soil microbes. These include pathogens, and bacteria that produce vitamins and protect against attack. The most common of the mycorrhizae are divided into the following: (1) ectomycorrhizae; (2) endomycorrhizae; (3) arbuscular mycorrhizae; (4) ericoid mycorrhizae; and (5) orchid mycorrhiza. The role of saprophytes, pathogens and actinomycetes are also discussed.

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Singh, Archana, and Ajit Varma. "Orchidaceous Mycorrhizal Fungi." In Mycorrhizal Biology, 265–88. Boston, MA: Springer US, 2000. http://dx.doi.org/10.1007/978-1-4615-4265-0_17.

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Satapathy, Sucharita, Shuvendu Shekhar Mohapatra, Puranjoy Sar, Ankita Priyadarshini, Debasis Mitra, and Subhadra Pattanayak. "AM Fungi." In Arbuscular Mycorrhizal Fungi, 155–62. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003354086-20.

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Ray, Anuprita, and Shuvendu Shekhar Mohapatra. "Arbuscular Mycorrhizal Fungi." In Arbuscular Mycorrhizal Fungi, 19–26. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003354086-3.

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Alloun, Wiem, and Debasis Mitra. "Arbuscular Mycorrhizal Fungi." In Arbuscular Mycorrhizal Fungi, 27–34. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003354086-4.

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Pagano, Marcela C., and Partha P. Dhar. "Arbuscular mycorrhizal fungi." In Biotechnology of Bioactive Compounds, 225–43. Chichester, UK: John Wiley & Sons, Ltd, 2015. http://dx.doi.org/10.1002/9781118733103.ch9.

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Gupta, Vandana, and T. Satyanarayana. "Molecular Genetics of Ectomycorrhizal Fungi." In Mycorrhizal Biology, 119–34. Boston, MA: Springer US, 2000. http://dx.doi.org/10.1007/978-1-4615-4265-0_8.

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Shekhar Mohapatra, Shuvendu, Anuprita Ray, Sonali Panda, Sucharita Satapathy, and Nutan Moharana. "Arbuscular Mycorrhizal Fungi and Their Association for Bioremediation in Rice Cultivation." In Arbuscular Mycorrhizal Fungi, 123–29. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003354086-16.

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Djebaili, R., B. Farda, G. Capoani, G. Pagnani, and M. Pellegrini. "Rice Seed Priming with AMF and AMF-Associated Bacteria for Crop Enhancement." In Arbuscular Mycorrhizal Fungi, 171–78. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003354086-22.

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González Henao, Sarah, and Thaura Ghneim-Herrera. "Arbuscular Mycorrhizal Fungi Role in Bioremediation in Rice in the Context of Climate Change." In Arbuscular Mycorrhizal Fungi, 187–92. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003354086-24.

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Тези доповідей конференцій з теми "Mycorrhizal fungi"

Diasamidze, Inga, Gia Bolkvadze, Natela Varshanidze, and Nana Zarnadze. "MACROMYCETES OF BEECH FOREST IN MTIRALA NATIONAL PARK, GEORGIA." In 24th SGEM International Multidisciplinary Scientific GeoConference 2024, 375–82. STEF92 Technology, 2024. https://doi.org/10.5593/sgem2024/3.1/s14.44.

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This review provides an overview of the diversity of macromycetes (fungi with visible fruiting bodies) in Mtirala national park beech forests, highlighting their ecological roles and significance within these ecosystems. Mtirala National Park is a protected area in Adjara region, Western Georgia. It is part of the Colchic Rainforests and Wetlands, a UNESCO World Heritage Site. Mtirala National Park Colchic broad-leaved and mixed forests include sweet chestnut and Oriental beech woods. Mount Mtirala is one of the most humid areas in the country. The fungal community in beech forests encompasses a wide range of taxa, including edible mushrooms, wood-decay fungi, mycorrhizal associates, and decomposers. Hypsizygus tessellatus (Beech Mushroom), Armillaria mellea (Honey Fungus), and Fomes fomentarius (Tinder Fungus) are among the common species found in these forests, contributing to nutrient cycling and decomposition processes. Furthermore, mycorrhizal fungi such as Ganoderma spp. (Reishi Mushrooms) and Phellinus spp. (Cracked-cap Polypore) form symbiotic relationships with beech trees, enhancing their nutrient uptake and overall health. The presence of Laetiporus sulphureus (Chicken of the Woods) and Xylaria polymorpha (Dead Man's Fingers) on decaying wood further highlights the dynamic interactions between fungi and their substrates in beech forests. Understanding the diversity and ecological roles of macromycetes in beech forests is crucial for conservation efforts and sustainable forest management practices. Continued research into these fungi will provide valuable insights into the functioning and resilience of beech forest ecosystems in the face of environmental changes and anthropogenic disturbances.

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Zhang, Shufan, Thirimachos Bourlai, and Jonathan Arnold. "MycorrhiSEE: a High-Resolution Image Dataset for Deep Learning Based Quantification of Arbuscular Mycorrhizal Fungi." In 2024 IEEE International Conference on Big Data (BigData), 8879–81. IEEE, 2024. https://doi.org/10.1109/bigdata62323.2024.10825578.

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Raffaele, Giulia, Marilena Ronzan, Carlo Filippeschi, Emanuela Del Dottore, and Barbara Mazzolai. "Plant Root Growth Responses to Mycorrhizal Fungi: In Vivo Characterization Through Time-Series Microscopy Analysis." In 2024 IEEE International Workshop on Metrology for Agriculture and Forestry (MetroAgriFor), 632–36. IEEE, 2024. https://doi.org/10.1109/metroagrifor63043.2024.10948764.

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Sirait, Gloria, Ashar Hasairin, and Syahmi Edi. "Identification of Mycorrhizal Fungi Spore in Environment of Medan State University." In The 4th International Conference on Science and Technology Applications. Switzerland: Trans Tech Publications Ltd, 2023. http://dx.doi.org/10.4028/p-dm2oq0.

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Mycorrhizal is a the symbiotic association between the roots of higher plants and the mycelium of certain fungi. Mycorrhizal has benefits for improving plant nutrition and increasing growth, as biological protection, involved in the Bio-Geo-Chemical cycle, increasing nutrient absorption from the soil to increase plant resistance to extreme drought and humidity. Research on mycorrhizal spores was carried out in 3 places with high levels of mycorrhizal diversity, humid areas, and lush trees, namely in the forest of the Medan State University campus, Jl. Williem Iskandar, Kec. Medan Tebung City of Medan. This research is limited to knowing the diversity of mycorrhizal spores in the forest of Medan State University. Mycorrhizae observed on the roots of Swietenia macrophylla. Observations focused on the morphology of mycorrhizal spores observed under a microscope. The results showed that the mycorrhizal that were successfully identified and observed around the Medan State University Campus Forest were Gigaspora sp. 1 (10 x 40), Gigaspora sp. 2 (10 x 40), Acaulospora sp. 1 (10x40) Acaulospora sp. 2 (10 x 40), Glomus sp. 1 (10 x 40), Glomus sp. 2 (10 x 40), Glomus sp. 3 (10 x 40), Glomus sp. 4 (10 x 40), Glomus sp. 5 (10 x 40), Glomus sp. 5 (10 x 40), Gigaspora sp. 3 (10 x 40).

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Tervonen, Kaisa, Anna Oldén, and Panu Halme. "Mycorrhizal fungi in wood-pastures." In 5th European Congress of Conservation Biology. Jyväskylä: Jyvaskyla University Open Science Centre, 2018. http://dx.doi.org/10.17011/conference/eccb2018/107420.

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Geck, Kate. "Mycorrhizal Materialities Positioning the entanglement of human and machine intelligence." In 28th International Symposium on Electronic Art. Paris: Ecole des arts decoratifs - PSL, 2024. http://dx.doi.org/10.69564/isea2023-10-short-geck-mycorrhizal-materialities.

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SHORT PAPER. Machine intelligence is increasingly being used in the world with sometimes dramatic effects on human and other-than-human lives through its decision-making capacity. Much artificial intelligence (AI) and machine learning (ML) is built on metaphors that centre extraction, competition and control. These also position AI itself as a resource to be extracted and controlled, paving a troubling path for speculative futures where AI may gain emergent or ambiguous levels of sentience. These metaphors are part of a historical trend where humans place themselves above the other-than-human world, and this has formed the basis of an extractive and one-sided relationship with that world. In light of this, what new metaphors might we employ to platform the relationships between human and machine intelligences? Thinking through mycorrhizae could be a productive way to foreground the entangled, generative nature of exchange between human and machine intelligences. This paper will briefly explore metaphor in human-computer interaction (HCI) and AI, before making an offering to think about these things through the material of the mycorrhiza, a symbiotic site of exchange between plants and fungi. It will then briefly detail a creative project that has emerged from this mycorrhizal thinking to produce machine imagined textiles and embroideries. It then concludes with a call to embed relational thinking into future practices between human and machine intelligences in order to create more equitable and even mutualistic outcomes.

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Mazurek, B. G., and I. S. Zhebrak. "Features of mycorrhiza Trifolium pratense L. in various phytocenoses." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.166.

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In four meadow phytocenoses after the restoration of anthropogenic biotopes, a high degree of mycotrophy of Trifolium pratense was established. Arbuscular mycorrhizal fungi (arbuscules, vesicles, free and intra-root nonseptic mycelium) and dark-colored septic endophytic fungi (sporocarpies and free septic mycelium) were revealed in the roots of the studied plants.

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Favre-Godal, Q., L. Gourguillon, L. Riffault-Valois, A. Urbain, S. Lordel-Madeleine, K. Gindro, and P. Choisy. "Orchids and their mycorrhizal fungi: an insufficiently explored relationship." In 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. © Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-3399784.

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Kulaeva, O. A., E. A. Zorin, D. A. Romanyuk, M. L. Gordon, E. S. Gribchenko, O. Y. Shtark, A. M. Afonin, I. A. Tikhonovich, and V. A. Zhukov. "Characterization of pea (Pisum sativum L.) microRNAs." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.138.

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Pea microRNAs and their targets were identified, and their differential expression was analyzed during the development of symbiosis with rhizobia and mycorrhizal fungi, and under conditions of abiotic stress caused by cadmium.

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Abdurashytov, S. F., E. V. Puzanova, K. S. Gritsevich, A. A. Zubochenko, and V. K. Zakharova. "Study of the development of arbuscular mycorrhizal fungi in the saline soils of Crimea." In РАЦИОНАЛЬНОЕ ИСПОЛЬЗОВАНИЕ ПРИРОДНЫХ РЕСУРСОВ В АГРОЦЕНОЗАХ. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-15.05.2020.25.

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It is known that arbuscular mycorrhiza (AM) fungi are able to mitigate the effect of various negative environmental factors on plants. Therefore, the aim of our research was to determine the most active AM fungi populations in saline soils of the Crimean Peninsula to select stress-resistant isolates. The spores were isolated by wet sieving. Mycorrhization was visualized by staining with black ink and studied quantitatively under a stereomicroscope. In 2019, 15 soil-plant samples from 4 locations of the salinity soils were sampled: Lake Achi (AB) and the nearby agrocenosis (PAB), Koyashskoye (KO), Kirkoyashskoye (PKP) and agrocenosis near it (KP), Chokrak (CH). The highest content of chlorine anions (119.5 mg) and bicarbonate anions (610.0 mg) was noted in the area near Lake Kirkoyashskoye. The smallest salinity was observed at arable land KP (8.5 and 229.0 mg). Plowing led to a decrease in the number of AM fungi spores in the studied sections of PAB by 80.3% and KP by 47.6% compared with the AB and PKP. The frequency of occurrence and the intensity of mycorrhizal colonization in plant roots on the shores of Lakes Achi and Kirkoyashskoye were 70.0-72.3% and 28.0-43.9%, while the same for the plowing areas around them were lower by 28.5-54.1% and 18.0-32.2%, respectively. In our study, there was no strict correlation between the number of spores and the estimated environmental conditions. Soil-plant samples and the spores isolated from them were used as inoculums for obtaining new AM fungal isolates with a stress resistance property.

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Звіти організацій з теми "Mycorrhizal fungi"

Phillips, Donald, and Yoram Kapulnik. Using Flavonoids to Control in vitro Development of Vesicular Arbuscular Mycorrhizal Fungi. United States Department of Agriculture, January 1995. http://dx.doi.org/10.32747/1995.7613012.bard.

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Vesicular-arbuscular mycorrhizal (VAM) fungi and other beneficial rhizosphere microorganisms, such as Rhizobium bacteria, must locate and infect a host plant before either symbiont profits. Although benefits of the VAM association for increased phosphorous uptake have been widely documented, attempts to improve the fungus and to produce agronomically useful amounts of inoculum have failed due to a lack of in vitro production methods. This project was designed to extend our prior observation that the alfalfa flavonoid quercetin promoted spore germination and hyphal growth of VAM fungi in the absence of a host plant. On the Israeli side of the project, a detailed examination of changes in flavonoids and flavonoid-biosynthetic enzymes during the early stages of VAM development in alfalfa found that VAM fungi elicited and then suppressed transcription of a plant gene coding for chalcone isomerase, which normally is associated with pathogenic infections. US workers collaborated in the identification of flavonoid compounds that appeared during VAM development. On the US side, an in vitro system for testing the effects of plant compounds on fungal spore germination and hyphal growth was developed for use, and intensive analyses of natural products released from alfalfa seedlings grown in the presence and absence of microorganisms were conducted. Two betaines, trigonelline and stachydrine, were identified as being released from alfalfa seeds in much higher concentrations than flavonoids, and these compounds functioned as transcriptional signals to another alfalfa microsymbiont, Rhizobium meliloti. However, these betaines had no effect on VAM spore germination or hyphal growth i vitro. Experiments showed that symbiotic bacteria elicited exudation of the isoflavonoids medicarpin and coumestrol from legume roots, but neither compound promoted growth or germination of VAM fungi in vitro. Attempts to look directly in alfalfa rhizosphere soil for microbiologically active plant products measured a gradient of nod-gene-inducing activity in R. meliloti, but no novel compounds were identified for testing in the VAM fungal system in vitro. Israeli field experiments on agricultural applications of VAM were very successful and developed methods for using VAM to overcome stunting in peanuts and garlic grown in Israel. In addition, deleterious effects of soil solarization on growth of onion, carrot and wheat were linked to effects on VAM fungi. A collaborative combination of basic and applied approaches toward enhancing the agronomic benefits of VAM asociations produced new knowledge on symbiotic biology and successful methods for using VAM inocula under field conditions

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Melville, Alaina. Assessment of a Mycorrhizal Fungi Application to Treat Stormwater in an Urban Bioswale. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.3019.

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Ramírez Gómez, María Margarita, Urley Adrián Pérez Moncada, Diana Paola Serralde Ordóñez, and Andrea María Peñaranda Rolón. Effect of Arbuscular Mycorrhizal Fungi on vascular wilt control caused by Fusarium oxysporum in cape gooseberry plantlets. Corporación Colombiana de Investigación Agropecuaria - AGROSAVIA, 2016. http://dx.doi.org/10.21930/agrosavia.poster.2016.29.

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Colombia es uno de los productores de uchuva más grandes del mundo. Este estudio tuvo como objetivo evaluar el efecto de un inóculo de mezcla de HMA (Rhizoglomus sp y Acaulospora mellea), en condiciones de invernadero en plántulas de grosella, con el fin de reducir el impacto de Fusarium spp y la mejora del desarrollo de la planta.

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Cheeke, Tanya. An Evaluation of the Nontarget Effects of Transgenic Bacillus thuringiensis Maize on Arbuscular Mycorrhizal Fungi in the Soil Ecosystem. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.1027.

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Humphrey, Makala. The importance of establishing an abundant network of Arbuscular mycorrhizal fungi and sustainable agricultural practices that are crucial for population success. Ames (Iowa): Iowa State University, December 2023. http://dx.doi.org/10.31274/cc-20240624-1453.

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Clemens, Cody, Michael Bell, Emmi Felker-Quinn, Michael Bell, Cody Clemens, and Emmi Felker-Quinn. Applying mycorrhizal critical loads of nitrogen to US federal lands containing forested and Mediterranean shrubland ecosystems. National Park Service, 2024. http://dx.doi.org/10.36967/2305249.

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Mycorrhizal fungi (MF) play a crucial role within ecosystems by forming mutually symbiotic relationships where they share water, carbon, nutrients, and other benefits with plants they have inoculated. Since these plant communities depend on MF for survival, the status of MF communities can be used as a proxy for the health of the plants they support. Anthropogenic nitrogen (N) deposition can disrupt the symbiotic relationship between the plants and MF by causing a shift in mycorrhizal community composition, and in turn a shift in the plant structure within an ecosystem. Lilleskov et al. (2019) summarized recent research on critical loads (CLs) of N for a shift from N-sensitive to N-tolerant mycorrhizal and tree communities, setting CLs to protect conifer-associated MF (5?6 kg-N ha-1 yr-1), and broadleaf-associated MF (10?20 kg-N ha-1 yr-1). Allen et al. (2016) developed CLs of N for shifts in arbuscular MF communities in California coastal sage scrub which contribute to the conversion of native shrubland to exotic annual grassland (10?11 kg-N ha-1 yr-1). Here we apply the CL-N for forest mycorrhizae to forested areas in the contiguous United States (CONUS), and the CL-N for southern California shrubland mycorrhizae to shrublands and grasslands within the Mediterranean ecoregion of California. The National Atmospheric Deposition Program (NADP) Total Deposition model (TDep) for total N deposition from 2018?2020 (v2018.02) is used to calculate exceedances of the CLs and evaluate potential risk to forest health. The areas of exceedance are calculated for all units within CONUS managed by the Fish and Wildlife Service (FWS), National Park Service (NPS), and Forest Service (USFS). Within CONUS, 68% of federal land managed by the three agencies is forest: 76% is conifer, 17% is broadleaf, and 7% is a mixture of both tree communities. The USFS manages 93% of this forested land, and 30% of USFS forests are in exceedance of CLs-N for MF. The NPS manages a smaller portion of federal forested land at 6%, with exceedance occurring in 29% of that area. The FWS controls only 1% of forested land but has exceedance of CLs in 43% of that area. Within Mediterranean California (CA), 29% of federal land managed by the three agencies is shrubland or grassland. Federal shrublands are in exceedance of the CL-N for MF in 25% of the area. The USFS manages 91% of federal shrublands in Mediterranean CA, with 27% of USFS CA shrublands in exceedance of the CL. The NPS manages 8% of federal shrublands in Mediterranean CA, of which only 3% is experiencing exceedance. The FWS is only responsible for 1% federal shrublands in Mediterranean CA, with exceedance occurring in 22% of that area. This analysis shows the effects that N deposition has on forest and shrubland ecosystems occurring within federal lands across CONUS. Knowing the extent of exceedance observed within federal lands informs natural resource management. On smaller scales, this analysis enables federal land managers to better understand what is happening on their lands, effectively guiding local management decisions. On larger scales, the analysis outlines a portion of the overall effects that N deposition has on ecosystems within CONUS, providing policymakers with the scientific evidence required to support future policy actions aimed at protecting natural resources.

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Devos, Katrien, Jeff Bennetzen, Ali Missaoui, and Paul Schliekelman. Unraveling the Genetics of Two Key Biomass Traits that Differentiate Upland and Lowland Tetraploid Switchgrass Ecotypes, Colonization by Mycorrhizal Fungi and Frost Tolerance. Office of Scientific and Technical Information (OSTI), December 2020. http://dx.doi.org/10.2172/1735505.

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Kapulnik, Yoram, Maria J. Harrison, Hinanit Koltai, and Joseph Hershenhorn. Targeting of Strigolacatones Associated Pathways for Conferring Orobanche Resistant Traits in Tomato and Medicago. United States Department of Agriculture, July 2011. http://dx.doi.org/10.32747/2011.7593399.bard.

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This proposal is focused on examination of two plant interactions: parasitic with Orobanche, and symbiosis with arbuscular mycorrhiza fungi (AMF), and the involvement of a newly define plant hormones, strigolactones (SLs), in these plant interactions. In addition to strigolactones role in regulation of above-ground plant architecture, they are also known to be secreted from roots, and to be a signal for seed germination of the parasitic plants Orobanche. Moreover, secreted strigolactones were recognized as inducers of AMFhyphae branching. The present work was aimed at Generation of RNAi mutants of both tomato and Medicago, targeting multiple genes that may be involved in strigolactone production, carotenoid biosynthesis pathway, Pi signaling or other metabolic pathways, and hence affect AMF colonization and/or Orobanche resistance. Following the newly formed and existing RNAi mutants were examined for AMF colonization and Orobanche resistance. At the first phase of this project Orobanche seed germination assays and AMF colonization were examined in intact plants. These assays were shown to be effective and resulted with enhancement of Orobanche seed germination and AMF colonization in WT tomato plants, whereas roots of strigolactones impaired lines did not result with Orobanche seed germination and mycorrhiza colonization. Unexpectedly, root organ cultures (ROC) that were produced from the same wild type (WT) and mutant lines did not induce the Orobanche seed germination and AMFhyphal branching. This implies that under in vitro conditions ROC cultures are missing an important component for induction of Orobanche seed germination and AMFhyphal branching. In another line of experiments we have tested transgenic lines of Medicagotruncatula for AMFhuyphal branching and Orobanche seed germination assays. These lines included lines silenced for a GRAS transcription factor (RNAi 1845), an NBS-LRR type resistance gene (RNAi 1847), a kinase (RNAi 2403) and a protein of unknown function (RNAi 2417). In all cases, five independent transgenic root lines showed altered AMFphenotypes with reduced or aberrant colonization patterns. Following, we transformed tomato plants with the M. truncatulaTC 127050 PhosphoinositidekinaseRNAi construct. Transgenic lines that contained GUS constructs were used as control. All transgenic lines showed reduced level of Orobanche seed germination, masking any strigoalctones-specific effect. The research demonstrated that SLs production may not be examined in ROC –based bioassays. It was shown by the 3 independent assays employed in this project that none of the recognized characters of SLs may be reflected in these bioassays. However, when the whole plant root exudates were examined, SLs activity in root exudates was demonstrated. Hence, it can be concluded that the presence of an intact shoot, and possibly, shoot factors, may be necessary for production of SLs in roots. Another point of interest that rises from these results is that the presence of SLs is not necessary for AMF completion of life cycle. Hence, it may be concluded that SLs are important for AMFhyphal branching, before symbiosis, but not essential for AMF colonization and life cycle completion under ROC system conditions.

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Use of Agricultural Drones for Application of Arbuscular Mycorrhizal Fungi Inoculants on Transplanted Rice Seedlings and Its Performance on Growth and Productivity. Food and Fertilizer Technology Center for the Asian and Pacific Region, November 2024. http://dx.doi.org/10.56669/meyq8963.

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The Growth of Calliandra calothyrsus Inoculated with Arbuscular Mycorrhizae Fungi on Silica Post Mining Media Amended with Soil Ameliorant. Food and Fertilizer Technology Center for the Asian and Pacific Region, December 2022. http://dx.doi.org/10.56669/lwuo5639.

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