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Готові списки джерел за темами / Nuclear DNA amount / Статті в журналах

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Статті в журналах з теми "Nuclear DNA amount"

Автор: Grafiati

Опубліковано: 3 червня 2025

Оновлено: 31 липня 2025

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1

Samuel, Rosabelle, J. B. Smith, and M. D. Bennett. "Nuclear DNA variation in Piper (Piperaceae)." Canadian Journal of Genetics and Cytology 28, no. 6 (1986): 1041–43. http://dx.doi.org/10.1139/g86-145.

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Анотація:

Significant interspecific variation in DNA amount was observed among nine species of Piper. The DNA amount of a wild octoploid accession of Piper nigrum was approximately double that of the cultivated tetraploid variety of the same species. However, three New World diploid species had higher nuclear DNA amounts than several New or Old World tetraploid species. The DNA content per basic genome was, on the whole, lower in cultivated than in wild species.Key words: Piperaceae, Piper, Piper nigrum (black pepper), polyploids, DNA amounts.

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2

Lawrence, M. E. "Senecio L. (Asteraceae) in Australia: Nuclear DNA amounts." Australian Journal of Botany 33, no. 2 (1985): 221. http://dx.doi.org/10.1071/bt9850221.

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Nuclear DNA amounts of 28 species of Senecio native to Australia and of four exotic species are reported. DNA amounts per 4C nucleus range from 4.22 pg to 42.90 pg, a 10-fold difference. Some of the variation is due to polyploidy as chromosome numbers range from n = 5 (by dysploid reduction) to n = 50, but DNA amounts per genome also vary from 0.84pg to 4.69 pg, a 6-fold difference. DNA amounts correlate with cell size and, in some instances, the size of structures. There is also a correlation between DNA amount and generation length which corresponds with previous observations. The possible d

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3

Patil, Chandrashekhar G. "Nuclear DNA Amount Variation in Cyamopsis D.C. (Fabaceae)." CYTOLOGIA 69, no. 1 (2004): 59–62. http://dx.doi.org/10.1508/cytologia.69.59.

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4

Colombo, M., and F. Tomé. "Nuclear DNA Amount and Aconitine Content inAconitum napellusSubspecies." Planta Medica 59, S 1 (1993): A696. http://dx.doi.org/10.1055/s-2006-959987.

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5

BENNETT, M. "Nuclear DNA Amount and Genome Size in Angiosperms." Annals of Botany 82 (December 1998): 1. http://dx.doi.org/10.1006/anbo.1998.0788.

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6

Eilam, T., Y. Anikster, E. Millet, J. Manisterski, and M. Feldman. "Nuclear DNA amount and genome downsizing in natural and synthetic allopolyploids of the genera Aegilops and Triticum." Genome 51, no. 8 (2008): 616–27. http://dx.doi.org/10.1139/g08-043.

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Recent molecular studies in the genera Aegilops and Triticum showed that allopolyploidization (interspecific or intergeneric hybridization followed by chromosome doubling) generated rapid elimination of low-copy or high-copy, non-coding and coding DNA sequences. The aims of this work were to determine the amount of nuclear DNA in allopolyploid species of the group and to see to what extent elimination of DNA sequences affected genome size. Nuclear DNA amount was determined by the flow cytometry method in 27 natural allopolyploid species (most of which were represented by several lines and each

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7

SETOYAMA, Makoto, Shinichi YOTSUMOTO, Tetsu HAMADA, et al. "Nuclear DNA amount in the cells involved in photokeratosis." Skin Cancer 4, no. 1 (1989): 109–13. http://dx.doi.org/10.5227/skincancer.4.109.

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8

Biradar, D. P., D. G. Bullock, and A. Lane Rayburn. "Nuclear DNA amount, growth, and yield parameters in maize." Theoretical and Applied Genetics 88, no. 5 (1994): 557–60. http://dx.doi.org/10.1007/bf01240918.

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9

Porter, H. L., and A. Lane Rayburn. "B-chromosome and C-band heterochromatin variation in Arizona maize populations adapted to different altitudes." Genome 33, no. 5 (1990): 659–62. http://dx.doi.org/10.1139/g90-097.

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The B-chromosome and C-band numbers were determined in 12 Arizona Indian maize populations. These populations were originally collected from altitudes ranging from 100 to 5300 ft (1 ft = 0.3048 m). In addition, the total nuclear DNA amounts of these populations have been observed to vary by as much as 20%. The number of B-chromosomes was not significantly correlated with altitude, C-band number, or nuclear DNA amount. C-band number was significantly correlated with both altitude and genome size. It does not appear that the amount of C-band variation can account for the large nuclear DNA variat

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10

Oh, H. J., M. K. Kim, G. Jang, et al. "48 THE AMOUNT OF TELOMERIC DNA IN LYMPHOCYTES OF DOG CLONES." Reproduction, Fertility and Development 20, no. 1 (2008): 104. http://dx.doi.org/10.1071/rdv20n1ab48.

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Controversy regarding the restoration of eroded telomere length of donor cells after the nuclear transfer process has arisen from previous studies of cloned cattle, mice, and pigs. Little is known about telomere lengths in dogs from either natural breeding or somatic cell nuclear transfer (SCNT). In this study, we investigated the amount of telomeric DNA (ATD) in the lymphocytes of growing dog clones and their somatic cell donors. One cloned male Afghan hound dog [Snuppy (Lee et al. 2005 Nature 436, 641)] and 3 cloned female Afghan hound dogs (Jang et al. 2006 Theriogenology; doi:10.1016J.THER

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11

Welle, Stephen, Kirti Bhatt, Bharati Shah, Nancy Needler, Joseph M. Delehanty, and Charles A. Thornton. "Reduced amount of mitochondrial DNA in aged human muscle." Journal of Applied Physiology 94, no. 4 (2003): 1479–84. http://dx.doi.org/10.1152/japplphysiol.01061.2002.

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Muscle concentrations of mRNAs encoded by mitochondrial DNA (mtDNA) decline with aging. To determine whether this can be explained by diminished mtDNA levels, we measured the relative concentrations of mtDNA and a representative mtDNA transcript [encoding cytochrome- coxidase, subunit 2 (COX-2)] in muscle of young (21–27 yr) and older subjects (65–75 yr). The amount of COX-2 mRNA (relative to 28S rRNA) was 22% lower ( P = 0.04) in older muscle, and the amount of mtDNA (relative to nuclear DNA) was 38% lower ( P = 0.0002). The average level of mitochondrial transcription factor A (Tfam), a prot

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12

Bennett, Simon T., and Michael D. Bennett. "Variation in nuclear DNA amount between wild and cultivated populations of Milium effusum (2n = 28)." Genome 35, no. 6 (1992): 1050–53. http://dx.doi.org/10.1139/g92-161.

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Анотація:

Mean 2C DNA amounts varied by 35.6%, ranging from 7.52 to 10.20 pg, between 10 populations of the grass Milium effusum L. Such intraspecific variation occurred despite a constant chromosome number (2n = 28) and no obvious differences in karyotype. Plants originating from botanic garden populations growing in cultivation had significantly (P < 0.001) larger DNA amounts than plants collected from wild populations. Moreover, variation in DNA amount within either the "cultivated" or the "wild" groups was not significant. As the environment in which plants are kept in botanic gardens is clearly

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13

Ricroch, A., R. Yockteng, S. C. Brown, and S. Nadot. "Evolution of genome size across some cultivated Allium species." Genome 48, no. 3 (2005): 511–20. http://dx.doi.org/10.1139/g05-017.

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Allium L. (Alliaceae), a genus of major economic importance, exhibits a great diversity in various morphological characters and particularly in life form, with bulbs and rhizomes. Allium species show variation in several cytogenetic characters such as basic chromosome number, ploidy level, and genome size. The purpose of the present investigation was to study the evolution of nuclear DNA amount, GC content, and life form. A phylogenetic approach was used on a sample of 30 Allium species, including major vegetable crops and their wild allies, belonging to the 3 major subgenera Allium, Ameralliu

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14

Li, Dandan, Guangyu Luo, Shuiliang Guo, et al. "Nuclear DNA Amounts in Chinese Bryophytes Estimated by Flow Cytometry: Variation Patterns and Biological Significances." Plants 12, no. 7 (2023): 1564. http://dx.doi.org/10.3390/plants12071564.

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There exists an obvious gap in our knowledge of the nuclear DNA amount of bryophytes, not only in terms of the low number of species represented, but also in systematic and geographic representation. In order to increase our knowledge of nuclear DNA amounts and variation patterns in bryophytes, and their potential phylogenetic significances and influences on phenotypes, we used flow cytometry to determine the DNA 1C values of 209 bryophyte accessions, which belong to 145 mosses and 18 liverworts collected from China, by using Physcomitrella patens as a standard. We quantified the differences i

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15

Al-Saheal, Y. A., and A. S. Larik. "Genetic control of environmentally induced DNA variation in flax genotrophs." Genome 29, no. 4 (1987): 643–46. http://dx.doi.org/10.1139/g87-108.

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Heritable changes can be induced in the amount of nuclear DNA of the Stormont Cirrus variety (PL) of flax when grown in the specific environments of nitrogen and phosphorus, but such changes did not occur in variety Royal (R). Changes occurred in the amount of nuclear DNA of R when the PL nuclear and cytoplasmic factors were introduced by crossing. In the experiments reported here, tests were made on the plasticity of the F1's of their reciprocal crosses to PL and R to understand why L and S genotrophs are stable. The results suggest that there may be a feedback from nucleus to cytoplasm in L0

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16

Berlyn, Graeme P., Ambrose O. Anoruo, Richard C. Beck, and Jianping Cheng. "DNA content polymorphism and tissue culture regeneration in Caribbean pine." Canadian Journal of Botany 65, no. 5 (1987): 954–61. http://dx.doi.org/10.1139/b87-132.

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Анотація:

The three varieties of Caribbean pine have significantly different amounts of nuclear DNA: Pinus caribaea var. caribaea, 11.5; P. caribaea var. hondurensis, 21; and, P. caribaea var. bahamensis, 25 pg. Dormant embryos of the three varieties had more nuclear DNA than germinating seedlings, and this extra DNA was spread through several classes from 2C to 7C; however, upon germination the seedling DNA rapidly reorganizes into the 2C–4C distribution typical of diploid plants. DNA content polymorphism of the dormant embryos among the three varieties was directly correlated with the diploid amount o

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17

Çiçek, Mehmet, AHMET EMRE YAPRAK, and ALİ RAMAZAN ALAN. "Morphometric and flow cytometric evaluations of a putative natural hybrid of Centaurium (Gentianaceae) from Turkey." Phytotaxa 204, no. 1 (2015): 22. http://dx.doi.org/10.11646/phytotaxa.204.1.2.

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A putative natural hybrid of Centaurium was discovered in a population composed of C. serpentinicola and C. tenuiflorum subsp. tenuiflorum in the vicinity of Köyceğiz (Muğla province, SW Turkey). Morphometric analysis performed using 14 diagnostic characters supported that the putative natural hybrid as an interspecific hybrid between C. serpentinicola and C. tenuiflorum subsp. tenuiflorum. Flow cytometric measurements revealed that C. serpentinicola and C. tenuiflorum subsp. tenuiflorum have similar nuclear DNA amounts (2.61 and 2.53 pg DNA/2C), whereas the putative hybrid have a significantl

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18

Oliveira, Natália Padilha de, Gabriel de Siqueira Gesteira, Maria do Socorro Padilha de Oliveira, and Lisete Chamma Davide. "Interphase nuclei, karyotypes and nuclear DNA amounts in five species of Oenocarpus (Arecaceae)." Comparative Cytogenetics 18 (May 8, 2024): 59–72. http://dx.doi.org/10.3897/compcytogen.18.117597.

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The genus Oenocarpus Martius, 1823 (Arecaceae) includes five species commonly used in Amazonia, especially for their fruits. Little is known about the cytogenetic characteristics and DNA amounts of these species, except for O. bataua (Martius, 1823). This study characterized and compared the types of interphase nuclei, the chromosome sets, and estimated the nuclear DNA amounts of Oenocarpus bacaba (Martius, 1823), O. bataua, O. distichus (Martius, 1823), O. mapora (H. Karsten, 1857) and O. minor (Martius, 1823). Standard cytogenetic analyses and estimates of the nuclear DNA amount by flow cyto

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19

Litvinchuk, Spartak N., Jury M. Rosanov, and Leo J. Borkin. "Correlations of geographic distribution and temperature of embryonic development with the nuclear DNA content in the Salamandridae (Urodela, Amphibia)." Genome 50, no. 4 (2007): 333–42. http://dx.doi.org/10.1139/g07-010.

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We used flow cytometry to measure the nuclear DNA content in erythrocytes of 27 salamandrid species. Across these species, diploid genome size varied more than 2 fold (51.3–104.4 pg). According to genome size and geographic distribution, 3 groups of newt species were recognized: West Palearctics with smaller amounts of nuclear DNA; Nearctic, with intermediate values; and East Asiatic, with higher genome sizes. Viviparous West Palearctic salamanders differed from most of the oviparous West Palearctic newts in possessing larger genome sizes. The nuclear DNA content strongly correlates with speci

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20

Bauer, Tobias, Katja Kirschbaum, Silvia Panter, Marion Kenk, and Jörg Bergemann. "Sensitive Detection of Soy (Glycine max) by Real-Time Polymerase Chain Reaction Targeting the Mitochondrial atpA Gene." Journal of AOAC INTERNATIONAL 94, no. 6 (2011): 1863–73. http://dx.doi.org/10.5740/jaoacint.10-257.

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Abstract Detection of trace amounts of allergens is essential for correct labeling of food products by the food industry. PCR-based detection methods currently used for this purpose are targeting sequences of DNA present in the cell nucleus. In addition to nuclear DNA, a substantial amount of mitochondrial DNA (mtDNA) copies are present in the cytoplasm of eukaryotic cells. The nuclear DNA usually consists of a set of DNA molecules present in two copies per cell, whereas mitochondrial DNA is present in a few hundred copies per cell. Thus, an increase in sensitivity can be expected when mtDNA i

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21

Ohri, D., and M. Pal. "The origin of chickpea (Cicer arietinum L.): karyotype and nuclear DNA amount." Heredity 66, no. 3 (1991): 367–72. http://dx.doi.org/10.1038/hdy.1991.46.

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22

Thibault, Jérôme. "Nuclear DNA amount in pure species and hybrid willows (Salix): a flow cytometric investigation." Canadian Journal of Botany 76, no. 1 (1998): 157–65. http://dx.doi.org/10.1139/b97-153.

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Flow cytometry (FCM) has been used to estimate the nuclear DNA content of 11 Salix species and 5 hybrids. One hundred and sixty nine individuals were studied including 159 individuals from a sequence of 32 communities along a stretch of river in France and 10 individuals from French and English collections for comparison. Isolated nuclei were stained with propidium iodide. FCM was a significantly more practical and rapid technique than that of establishing the karyotype to survey many samples of Salix for variation in ploidy. The 2C DNA amounts for diploid species ranged from 0.76 to 0.98 pg,

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23

Watson, E. M. "Nuclear DNA content in the Australian Bulbine (Liliaceae)." Genome 29, no. 2 (1987): 225–34. http://dx.doi.org/10.1139/g87-040.

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Nuclear DNA measurements for 17 Australian populations of Bulbine support the recognition of the four major groups already suggested by chromosome studies. Within the perennial group, Bulbine bulbosa s. lat., the tuberless, 46-chromosome (hypo-8x) "rock lily" has a similar DNA content to the hypo-4x, 24-chromosome populations of the "bulbosa" complex, which has populations at hypo-4x, -8x, and -12x levels. The "rock lily" also has substantially less DNA than another 46-chromosome entity, represented by the Kroombit population. Within the annual group, Bulbine semibarbata s. lat., the distincti

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24

Robey, F. A., K. D. Jones, and A. D. Steinberg. "C-reactive protein mediates the solubilization of nuclear DNA by complement in vitro." Journal of Experimental Medicine 161, no. 6 (1985): 1344–56. http://dx.doi.org/10.1084/jem.161.6.1344.

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We have studied the interaction of C-reactive protein (CRP)-chromatin complexes with serum. The amount of chromatin solubilized by serum is directly proportional to the amount of CRP present. Serum minus C3 did not appreciably solubilize chromatin within the time allowed in these experiments regardless of the amount of CRP present. This indicates that, in addition to CRP, complement is critical to the solubilization process. Studies using genetically C2-deficient serum and purified C2 indicate that the classical complement pathway is primarily involved in the solubilization, however, there may

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25

Nguyen, Lap P., Julius Svensmark, Xin Jiang, Alexander Jordan, and Cord Brakebusch. "Nuclear RhoA Activation Regulates Nucleus Size and DNA Content via Nuclear Activation of ROCK and pErk." Cells 14, no. 6 (2025): 404. https://doi.org/10.3390/cells14060404.

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RhoA is a major regulator of the actin cytoskeleton. Its function in the nucleus, however, is unclear. Fusing wildtype, fast cycling, constitutively active, and dominant negative forms of RhoA with tags promoting nuclear or cytoplasmic location and allowing specific detection, we established a platform to distinguish the functions of nuclear and cytoplasmic RhoA. Our data show that nuclear but not cytoplasmic activation of RhoA regulates DNA amount and nuclear size. This is mediated by sequential nuclear activation of the RhoA effector ROCK and Erk, a major cell cycle regulating kinase. The in

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26

Oliveira, Natália Padilha de, Gabriel de Siqueira Gesteira, de Oliveira Maria do Socorro Padilha, and Lisete Chamma Davide. "Interphase nuclei, karyotypes and nuclear DNA amounts in five species of Oenocarpus (Arecaceae)." Comparative Cytogenetics 18 (May 8, 2024): 59–72. https://doi.org/10.3897/compcytogen.18.117597.

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Анотація:

The genus <i>Oenocarpus</i> Martius, 1823 (Arecaceae) includes five species commonly used in Amazonia, especially for their fruits. Little is known about the cytogenetic characteristics and DNA amounts of these species, except for <i>O. bataua</i> (Martius, 1823). This study characterized and compared the types of interphase nuclei, the chromosome sets, and estimated the nuclear DNA amounts of <i>Oenocarpus bacaba</i> (Martius, 1823), <i>O. bataua</i>, <i>O. distichus</i> (Martius, 1823), <i>O. mapora</i> (H. Karsten, 1857) and <i>O. minor</i> (Martius, 1823). Standard cytogenetic analyses and

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27

Eilam, T., Y. Anikster, E. Millet, J. Manisterski, O. Sagi-Assif, and M. Feldman. "Genome size and genome evolution in diploid Triticeae species." Genome 50, no. 11 (2007): 1029–37. http://dx.doi.org/10.1139/g07-083.

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Анотація:

One of the intriguing issues concerning the dynamics of plant genomes is the occurrence of intraspecific variation in nuclear DNA amount. The aim of this work was to assess the ranges of intraspecific, interspecific, and intergeneric variation in nuclear DNA content of diploid species of the tribe Triticeae (Poaceae) and to examine the relation between life form or habitat and genome size. Altogether, 438 plants representing 272 lines that belong to 22 species were analyzed. Nuclear DNA content was estimated by flow cytometry. Very small intraspecific variation in DNA amount was found between

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28

Hayward, Kristen M., Michelle P. Harwood, Stephen C. Lougheed, Zhengxin Sun, Peter Van Coeverden de Groot, and Evelyn L. Jensen. "A real-time PCR assay to accurately quantify polar bear DNA in fecal extracts." PeerJ 8 (April 7, 2020): e8884. http://dx.doi.org/10.7717/peerj.8884.

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DNA extracted from fecal samples contains DNA from the focal species, food, bacteria and pathogens. Most DNA quantification methods measure total DNA and cannot differentiate among sources. Despite the desirability of noninvasive fecal sampling for studying wildlife populations, low amounts of focal species DNA make it difficult to use for next-generation sequencing (NGS), where accurate DNA quantification is critical for normalization. Two factors are required prior to using fecal samples in NGS libraries: (1) an accurate quantification method for the amount of target DNA and (2) a determinat

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29

Cremonini, R., M. Ruffini Castiglione, G. Venora, S. Blangiforti, F. P. Losavio, and D. Pignone. "Cytology ofViciaspecies. VI. Nuclear chromatin organization, karyomorphological analysis and DNA amount inVicia serratifoliaJacq." Caryologia 51, no. 3-4 (1998): 195–205. http://dx.doi.org/10.1080/00087114.1998.10797412.

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30

Srivastava, Sangeeta, and U. C. Lavania. "Evolutionary DNA variation in Papaver." Genome 34, no. 5 (1991): 763–68. http://dx.doi.org/10.1139/g91-118.

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Анотація:

In 23 species of Papaver L., 2C nuclear DNA amounts range from 4.64 pg in Papaver persicum (2n = 14) to 22.43 pg in Papaver orientale (2n = 42), revealing a fivefold variation within the genus. However, such variation is limited to only twofold among the species that have the same chromosome number (2n = 14). The distribution of DNA is discontinuously spread over six groups in the genus. A strong positive correlation exists between nuclear DNA content and metaphase chromosome length. Viewed in the context of evolutionary divergence, it is revealed that DNA reduction has taken place in conjunct

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31

Sen, Jayanti, Sumona Mukherjee, and A. K. Sharma. "Study of chromosomes, DNA amount, and in vitro growth in different species of Luzula." Genome 33, no. 1 (1990): 143–47. http://dx.doi.org/10.1139/g90-022.

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Cells of six different species of Luzula were subjected to in vitro culture and examined for callus formation. The cells of calli from the different species were analyzed for variation in chromosome number. The cells of different species showed a differential response in culture with respect to callus growth. Chromosome number variation in vitro, including both hypo- and hyper-diploidy, was recorded in all species except L. pediformis and L. luzuloides. The chromosome fragments survived as a result of a nonlocalized centromere. With prolonged culture, normal diploid cells were frequent. The nu

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32

Garcia, Sònia, María Sanz, Teresa Garnatje, Agnieszka Kreitschitz, E. Durant McArthur, and Joan Vallès. "Variation of DNA amount in 47 populations of the subtribe Artemisiinae and related taxa (Asteraceae, Anthemideae): karyological, ecological, and systematic implications." Genome 47, no. 6 (2004): 1004–14. http://dx.doi.org/10.1139/g04-061.

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Анотація:

Genome size has been estimated by flow cytometry in 47 populations of 40 species of the tribe Anthemideae (Asteraceae), mainly from Artemisia and other genera of the subtribe Artemisiinae and related taxa. A range of 2C values from 3.54 to 21.22 pg was found. DNA amount per basic chromosome set ranged from 1.77 to 7.70 pg. First genome size estimates are provided for one subtribe, 10 genera, 32 species, and two subspecies. Nuclear DNA amount correlated well with some karyological, physiological and environmental characters, and has been demonstrated as a useful tool in the interpretation of ev

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33

Collas, Philippe, and Peter Aleström. "Nuclear localization signals: a driving force for nuclear transport of plasmid DNA in zebrafish." Biochemistry and Cell Biology 75, no. 5 (1997): 633–40. http://dx.doi.org/10.1139/o97-044.

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Nuclear localization signals (NLSs) are short peptides required for nuclear transport of karyophilic proteins. We review in this paper how the nuclear targeting property of NLS peptides has been taken advantage of to enhance the efficiency of nuclear uptake of transgene DNA in zebrafish and how it may improve the efficiency of transgenesis in this species. Synthetic NLS peptides can bind to plasmid DNA by ionic interactions. Cytoplasmic injection of DNA-NLS complexes in zebrafish eggs enhances the rate and the amount of plasmid DNA taken up by embryonic nuclei. Nuclear import of DNA-NLS comple

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34

Torrell, Montserrat, and Joan Vallès. "Genome size in 21 Artemisia L. species (Asteraceae, Anthemideae): Systematic, evolutionary, and ecological implications." Genome 44, no. 2 (2001): 231–38. http://dx.doi.org/10.1139/g01-004.

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Genome size was estimated by flow cytometry in 24 populations belonging to 22 Artemisia taxa (21 species, 1 with two subspecies), which represent the distinct subgenera, life forms, basic chromosome numbers, and ploidy levels in the genus. 2C nuclear DNA content values range from 3.5 to 25.65 pg, which represents a more than sevenfold variation. DNA content per haploid genome ranges from 1.75 to 5.76 pg. DNA amount is very well correlated with karyotype length and ploidy level. Some variations in genome size have systematic and evolutionary implications, whereas others are linked to ecological

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Riggio, Daniela C., Nicola Ricci, Rosalba Banchetti, and Hans Martin Seyfert. "Cannibals of Oxytricha bifaria (Ciliata, Hypotrichida). Macro- and micro-nuclear DNA content." Canadian Journal of Zoology 65, no. 4 (1987): 847–51. http://dx.doi.org/10.1139/z87-135.

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Investigation of cell differentiation led us to study the giants of Oxytricha bifaria. Here we report systematic variations in nuclear DNA content. Comparing giants, controls starved for 10–14 days, and controls we conclude that (i) the amount of DNA is indicative of the occurrence of cell differentiation, while cell size or nuclear size is not; (ii) the macronuclear DNA content of a giant is about 3.7 times that of the controls; and (iii) the micronuclear DNA content of a giant is about 1.8 times that of the controls. The macronuclear DNA content declines throughout the four successive divisi

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36

Eilam, T., Y. Anikster, E. Millet, J. Manisterski, and M. Feldman. "Genome Size in Diploids, Allopolyploids, and Autopolyploids of Mediterranean Triticeae." Journal of Botany 2010 (June 29, 2010): 1–12. http://dx.doi.org/10.1155/2010/341380.

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Nuclear DNA amount, determined by the flow cytometry method, in diploids, natural and synthetic allopolyploids, and natural and synthetic autopolyploids of the tribe Triticeae (Poaceae) is reviewed here and discussed. In contrast to the very small and nonsignificant variation in nuclear DNA amount that was found at the intraspecific level, the variation at the interspecific level is very large. Evidently changes in genome size are either the cause or the result of speciation. Typical autopolyploids had the expected additive DNA amount of their diploid parents, whereas natural and synthetic cyt

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Tatum, Tatiana C., Svetlana Stepanovic, D. P. Biradar, A. Lane Rayburn, and Schuyler S. Korban. "Variation in nuclear DNA content in Malus species and cultivated apples." Genome 48, no. 5 (2005): 924–30. http://dx.doi.org/10.1139/g05-033.

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The nuclear DNA content for a group of 40 Malus species and hybrids has been estimated using flow cytometry. Estimates of nuclear DNA content for this germplasm collection range from 1.45 pg for Malus fusca (diploid) to 2.57 pg for Malus ioensis (triploid). Among diploids, the nuclear (2C) DNA ranges from 1.45 pg for M. fusca to 1.68 pg for Malus transitoria. Among triploids, the nuclear (3C) DNA content ranges from 2.37 pg / 3C for Malus sikkimensis to 2.57 pg / 3C for M. ioensis. Given the complexity of the apple genome and its suggested allopolyploid origin, the results obtained in this stu

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38

Zyrianova, I. M. "Chicken nuclear DNA in chicken egg whites." Agrarian science, no. 9 (September 22, 2023): 38–42. http://dx.doi.org/10.32634/0869-8155-2023-374-9-38-42.

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Poultry eggs contain high-quality proteins, fats, vitamins and minerals, which makes them an indispensable product in human nutrition. Egg whites have exceptional foaming, emulsifying, gelling, and heat setting properties, making them popular for use in baked goods. The knowledge accumulated so far about egg whites has now settled on a stable opinion that there are no cells, and no DNA, in them. However, this study demonstrates the possibility of extracting chicken genomic DNA from the outer thin whites of chicken eggs. For DNA extraction, foremost, protein degradation of the samples by trypsi

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39

Reyes, J., L. F. Jiménez-García, M. A. Gonzalez, and J. M. Vázquez-Ramos. "Benzyladenine-stimulation of nuclear DNA synthesis and cell division in germinating maize." Seed Science Research 1, no. 2 (1991): 113–17. http://dx.doi.org/10.1017/s096025850000074x.

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AbstractWe have studied by means of cytology and autoradiography the effect of benzyladenine (BA, a synthetic cytokinin) on DNA metabolism during early maize germination.The data indicate that BA stimulates nuclear DNA replication. The doubling of the amount of nuclear DNA in BA-treated axes occurs earlier than in nontreated axes, and there is a three-fold increase in the mitotic index at 24 h of germination. These results provide further corroboration for the suggestion that the stimulation of DNA synthesis observed relates to a nuclear replicative type of synthesis. Possible mechanisms of ac

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40

Xavier, Catarina, Charlotte Sutter, Christina Amory, Harald Niederstätter, and Walther Parson. "NuMY—A qPCR Assay Simultaneously Targeting Human Autosomal, Y-Chromosomal, and Mitochondrial DNA." Genes 14, no. 8 (2023): 1645. http://dx.doi.org/10.3390/genes14081645.

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The accurate quantification of DNA in forensic samples is of utmost importance. These samples are often present in limited amounts; therefore, it is indicated to use the appropriate analysis route with the optimum DNA amount (when possible). Also, DNA quantification can inform about the degradation stage and therefore support the decision on which downstream genotyping method to use. Consequently, DNA quantification aids in getting the best possible results from a forensic sample, considering both its DNA quantity and quality limitations. Here, we introduce NuMY, a new quantitative real-time P

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41

Hosny, Michel, Vivienne Gianinazzi-Pearson, and Hubert Dulieu. "Nuclear DNA content of 11 fungal species in Glomales." Genome 41, no. 3 (1998): 422–28. http://dx.doi.org/10.1139/g98-038.

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The nuclear DNA content of 11 species of Glomales was evaluated by flow cytometry after DAPI staining relative to Gigaspora margarita, which was used as internal standard. The nuclear DNA content of this species was calibrated by propidium iodide staining relative to chicken red blood cells. A correction was applied when the difference in AT content of the DNA was significant between a sample and the standard. A single unimodal peak of fluorescence was observed for nuclei from the quiescent spores of the 11 fungal species studied. It was considered that this peak corresponded to the amount of

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42

Cavallini, A., L. Natali, T. Giordani, M. Durante, and P. G. Cionini. "Nuclear DNA changes within Helianthus annuus L.: variations in the amount and methylation of repetitive DNA within homozygous progenies." Theoretical and Applied Genetics 92-92, no. 3-4 (1996): 285–91. http://dx.doi.org/10.1007/bf00223670.

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43

Cavallini, A., L. Natali, T. Giordani, M. Durante, and P. G. Cionini. "Nuclear DNA changes within Helianthus annuus L.: variations in the amount and methylation of repetitive DNA within homozygous progenies." TAG Theoretical and Applied Genetics 92, no. 3-4 (1996): 285–91. http://dx.doi.org/10.1007/s001220050126.

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44

Kuta, Elzbieta, Borut Bohanec, Ewa Dubas, Liliana Vizintin, and Leslaw Przywara. "Chromosome and nuclear DNA study on Luzula - a genus with holokinetic chromosomes." Genome 47, no. 2 (2004): 246–56. http://dx.doi.org/10.1139/g03-121.

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Chromosomes and nuclear DNA amount were analyzed in leaf tissues of Luzula nivea, Luzula luzuloides, and Luzula multiflora. Intra- and interspecific karyological variability was stated. Chromosome numbers in diploids ranged 2n = 8-24 in L. nivea and L. luzuloides and 2n = 12-84 in hexaploid L. multiflora. Karyological variability resulted mainly from chromosome fission (agmatoploidy) and aneusomaty; chromosome fusion (symploidy) and polyploidy were also involved. Flow cytometric determination of nuclear genome size using propidium iodide staining gave values of 1.584 pg in L. luzuloides, 1.566

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45

Szepesi, A., EW Gelfand, and JJ Lucas. "Association of proliferating cell nuclear antigen with cyclin-dependent kinases and cyclins in normal and transformed human T lymphocytes." Blood 84, no. 10 (1994): 3413–21. http://dx.doi.org/10.1182/blood.v84.10.3413.3413.

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Abstract The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and appears to be needed for both DNA synthesis and DNA repair. It is present in low amount in resting normal human T lymphocytes and, upon mitogenic stimulation with phorbol dibutyrate and ionomycin, begins to increase in mid-G1 phase, approximately 12 to 15 hours before entry into S phase. PCNA continues to increase in amount throughout the cell cycle and remains high in proliferating cultures. PCNA was extracted from activated normal T cells and from the transformed T-lymphoblastoid cell l

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46

Szepesi, A., EW Gelfand, and JJ Lucas. "Association of proliferating cell nuclear antigen with cyclin-dependent kinases and cyclins in normal and transformed human T lymphocytes." Blood 84, no. 10 (1994): 3413–21. http://dx.doi.org/10.1182/blood.v84.10.3413.bloodjournal84103413.

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Анотація:

The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and appears to be needed for both DNA synthesis and DNA repair. It is present in low amount in resting normal human T lymphocytes and, upon mitogenic stimulation with phorbol dibutyrate and ionomycin, begins to increase in mid-G1 phase, approximately 12 to 15 hours before entry into S phase. PCNA continues to increase in amount throughout the cell cycle and remains high in proliferating cultures. PCNA was extracted from activated normal T cells and from the transformed T-lymphoblastoid cell line Jurka

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47

Eilam, T., Y. Anikster, E. Millet, J. Manisterski, and M. Feldman. "Genome size in natural and synthetic autopolyploids and in a natural segmental allopolyploid of several Triticeae species." Genome 52, no. 3 (2009): 275–85. http://dx.doi.org/10.1139/g09-004.

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Nuclear DNA amount (1C) was determined by flow cytometry in the autotetraploid cytotype of Hordeum bulbosum , in the cytologically diploidized autotetraploid cytotypes of Elymus elongatus , Hordeum murinum subsp. murinum and Hordeum murinum subsp. leporinum, in Hordeum marinum subsp. gussoneanum, in their progenitor diploid cytotypes, and in a newly synthesized autotetraploid line of E. elongatus. Several lines collected from different regions of the distribution area of every taxon, each represented by a number of plants, were analyzed in each taxon. The intracytotype variation in nuclear DNA

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Platzek, T., G. Bochert, U. Rahm, and D. Neubert. "Embryotoxicity Induced by Alkylating Agents. Some Methodological Aspects of DNA Alkylation Studies in Murine Embryos Using Ethylmethanesulfonate." Zeitschrift für Naturforschung C 42, no. 5 (1987): 613–26. http://dx.doi.org/10.1515/znc-1987-0519.

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Abstract Synthesis and spectroscopic analysis of some alkylated DNA purine bases are described. HPLC separation methods are developed for the determination of DNA alkylation rates in mammalian embryonic tissues. Following treatment of pregnant mice with the ethylating agent ethyl-methanesulfonate (EMS), an appreciable amount of alkylation (ethylation and methylation) was found in the nuclear DNA of the embryos during organogenesis. The results are discussed in context of our thesis that a certain amount of DNA alkylation in the embryos is correlated to the teratogenic potential of alkylating a

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49

Van Maele, Bénédicte, Jan De Rijck, Erik De Clercq, and Zeger Debyser. "Impact of the Central Polypurine Tract on the Kinetics of Human Immunodeficiency Virus Type 1 Vector Transduction." Journal of Virology 77, no. 8 (2003): 4685–94. http://dx.doi.org/10.1128/jvi.77.8.4685-4694.2003.

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ABSTRACT Lentiviral vectors derived from human immunodeficiency virus type 1 (HIV-1) show great promise as gene carriers for future gene therapy. Insertion of a fragment containing the central polypurine tract (cPPT) in HIV-1 vector constructs is known to enhance transduction efficiency drastically, reportedly by facilitating the nuclear import of HIV-1 cDNA through a central DNA flap. We have studied the impact of the cPPT on the kinetics of HIV-1 vector transduction by real-time PCR. The kinetics of total HIV-1 DNA, two-long-terminal-repeat (2-LTR) circles, and, by an Alu-PCR, integrated pro

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Mountz, J. D., and A. D. Steinberg. "Studies of c-myb gene regulation in MRL-lpr/lpr mice. Identification of a 5' c-myb nuclear protein binding site and high levels of binding factors in nuclear extracts of lpr/lpr lymph node cells." Journal of Immunology 142, no. 1 (1989): 328–35. http://dx.doi.org/10.4049/jimmunol.142.1.328.

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Abstract Lymph node T cells of MRL-lpr/lpr mice are characterized by the production of very large amounts of c-myb mRNA. To study the control of c-myb expression, a search was made for sites on the 5' c-myb gene which could bind regulatory proteins. DNase I digestion of nuclear chromatin uncovered four DNase I hypersensitive sites in the first intron of the c-myb gene, and a single site approximately 300 bp 5' to the initiation codon. Lambda exonuclease digestion of a 5'-myb fragment in the presence of nuclear extracts from either MRL-lpr/lpr PLN or EL-4 thymoma revealed stop sites approximate

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