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Дисертації з теми "Plant development, Microproteins, Molecular Biology"

Автор: Grafiati

Опубліковано: 11 березня 2023

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1

van, Zyl Albertha R. "Development of plant-produced Bluetongue virus vaccines." Doctoral thesis, University of Cape Town, 2014. http://hdl.handle.net/11427/28248.

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Bluetongue is a disease of domestic and wild ruminants caused by Bluetongue virus (BTV). It has caused several serious outbreaks, the most recent occurring in Northern Europe in 2006 during which high mortality rates of livestock were reported. The only vaccines currently approved and commercially available for use are live-attenuated or inactivated virus strains and although these are effective, there is the risk of reversion in the case of live-attenuated strains to more virulent forms by recombination. Another drawback associated with the use of live-attenuated virus vaccines is that they a

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2

Dennis, Susan Jennifer. "Development of plant-produced African horse sickness vaccines." Thesis, Faculty of Science, 2019. http://hdl.handle.net/11427/33687.

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African horse sickness is a devastating disease that causes great suffering and many fatalities amongst horses in sub-Saharan Africa. It is caused by nine different serotypes of the orbivirus African horse sickness virus (AHSV) and it is spread by Culicoid midges. The disease has significant economic consequences for the equine industry both in southern Africa and increasingly further afield as the geographic distribution of the midge vector broadens with global warming and climate change. Live attenuated vaccines (LAV) have been used with relative success for many decades, but carry the risk

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3

Cao, Jingyi. "CELL TYPE-SPECIFIC ALTERNATIVE POLYADENYLATION IN ARABIDOPSIS DURING DEVELOPMENT AND STRESS RESPONSE." Miami University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=miami1492702815819455.

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4

Hable, Whitney Elizabeth 1967. "Expression and regulation of phytoene desaturase during maize seed development." Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/282172.

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An essential component of development is the accumulation of specific metabolites in a temporal and tissue-specific manner. The growth regulator abscisic acid (ABA), which accumulates at a specific time during seed development, is required for seed maturation and prevents the premature developmental switch from dormancy to germination ABA accumulates differently in two tissues of the seed; levels in the embryo are several-fold higher than in the endosperm and the temporal accumulation of ABA is also different between these tissues. To begin to understand how ABA accumulation is regulated durin

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5

Murphy, Phillip James. "Plant-fungal interactions during vesicular-arbuscular mycorrhiza development : a molecular approach." Title page, contents and abstract only, 1995. http://web4.library.adelaide.edu.au/theses/09PH/09phm9778.pdf.

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Bibliography: leaves 153-185. Vesicular-arbuscular (VA) mycorrhiza formation is a complex process which is under the genetic control of both plant and fungus. This project aims to develop a model infection system in Hordeum vulgare L. (barley) suitable for molecular analysis; to identify host plant genes differentially expressed during the early stages of the infection process; and to screen a mutant barley population for phenotypes which form abnormal mycorrhizas.

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6

Douglas, Stephanie. "The development of molecular markers for use across all plant species using expressed sequence tags." FIU Digital Commons, 2006. http://digitalcommons.fiu.edu/etd/3234.

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There are over a half a million plant species on earth, and we use them in virtually every aspect of our lives. Little or no genomic information exists about the vast majority of these plants. This study investigated the use of Expressed Sequence Tags (ESTs) to locate highly conserved sequences from which to design a set of universal molecular markers for all plant species. Plant species for this study were chosen to representative of the plant kingdom. This was done by sampling several individuals of at least one species from all of the major terrestrial plant groups. Conserved sequences are

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7

Paddock, Troy N. "Genetic manipulation of NADPH: Protochlorophyllide Oxidoreductase content in Arabidopsis reveals essential roles in prolamellar body formation and plant development." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1211899658.

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8

Wehmeyer, Nadja. "Arabidopsis class I small heat shock proteins: Regulation and functional analysis during seed development." Diss., The University of Arizona, 1999. http://hdl.handle.net/10150/284011.

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The goal of this dissertation was to analyze the regulation and function of cytoplasmic class I small heat shock proteins (sHSPs) during seed development in Arabidopsis thaliana. Results show that two class I sHSPs accumulate in late seed maturation, persist in the dry seed and decline rapidly during germination. HSP17.4 accounts for 90% of total class I sHSP in the dry seed. The temporal pattern of sHSP accumulation during seed development suggests that HSP17.4 may help establish seed properties that are acquired during late seed maturation, such as dormancy or desiccation tolerance. Several

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9

Xu, Limin. "Development of molecular approaches in the study of lettuce downy mildew (Bremia lactucae) population biology." Thesis, University of Warwick, 2011. http://wrap.warwick.ac.uk/49561/.

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Downy mildew of lettuce caused by Bremia lactucae is a serious disease resulting in yield loss. The population structure of the pathogen in the UK is poorly understood. This PhD project concentrated on developing molecular markers to differentiate the genotypic variation of B. lactucae populations, with the aim of improving methods to investigate lettuce - Bremia interactions. Thirty-seven B. lactucae isolates (including single-spore and new field isolates) were collected and characterized for virulence using the conventional International Bremia Evaluation Board (IBEB) differential set. Micro

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10

Calixte, Sophie. "RNA processing of the ccmFn-rps1 and rpl5-Psirps14-cox3 loci in wheat mitochondria during seedling development." Thesis, University of Ottawa (Canada), 2008. http://hdl.handle.net/10393/27580.

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Plant mitochondria possess a gene expression system in which post-transcriptional events, such as transcript end maturation and turnover mechanisms play a key role in regulating the transcriptome. In addition, during early developmental stages of embryo germination, differing transcript profiles have been seen. This research focuses on two loci in wheat mitochondria, ccmFn-rps1 and rpl5-Psirps14-cox3, to elucidate the transcription and post-transcriptional events involved in their expression. Northern analysis of the ccmFN-rps1 genes during early seed-to-seedling development reveals a 3.2 kb

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11

Regnard, Guy Louis. "Development of a potential challenge model and plant-produced vaccine candidate for beak and feather disease virus." Doctoral thesis, University of Cape Town, 2015. http://hdl.handle.net/11427/15690.

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Psittacine beak and feather disease (PBFD), the most prevalent viral disease affecting psittacines, is caused by beak and feather disease virus (BFDV). An outbreak of the disease has been reported in wild endangered Cape parrots (Poicephalus robustus), which is endemic to South Africa. No treatment or vaccine is commercially available. In this study, an investigation into the outbreak was undertaken. BFDV diversity was assessed and viral load and clinical signs correlated. A plant-produced BFDV subunit vaccine was produced in parallel with a corresponding challenge model. Cape parrots were as

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12

Angobe, Aune Tuyoleni. "Development of a plant-made immunoassay for the detection of Porcine circovirus infections in South African swine herds." Master's thesis, Faculty of Science, 2021. http://hdl.handle.net/11427/33629.

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Porcine circovirus type 2 (PCV-2) is considered the major cause of porcine circovirusassociated diseases and is one of the major pathogens in swine producing countries. PCV-2 is a non-enveloped virus with a single stranded circular DNA genome of about 1.8 kb. This encodes the single capsid protein (CP) which is highly immunogenic, as well as a replication-associated protein. Recombinantly expressed CP can selfassemble into virus-like particles (VLPs) that are structurally and immunogenically very similar to native virions. Current commercially available diagnostic kits are VLPbased and are eff

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13

Hayashi, Satomi. "Molecular characterisation of early nodulation events in soybean." Thesis, The University of Queensland, 2014.

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Анотація:

Legumes include some of the most economically important crops and are characterised by their ability to form a symbiotic relationship with nitrogen fixing rhizobia bacteria. Effective legume-rhizobia symbioses result in the formation of a wart-like structure on the root called the nodule. In this nodule, the rhizobia provide nitrogen to the host plant in exchange for energy and shelter. Nodulation is induced by the recognition of a rhizobia derived, strain specific signal, called Nod factor. Recognition of Nod factor from compatible rhizobia leads to complex signalling within and between the e

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14

Sarrión, Perdigones Manuel Alejandro. "Design and development of modular DNA assembly tools for Multigene Engineering and Synthetic Biology in Plants." Doctoral thesis, Universitat Politècnica de València, 2014. http://hdl.handle.net/10251/35399.

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The post-genomics era has put at the disposal of modern plant breeders an endless list of genetic building blocks for the design of new biotechnological crops. After a first wave of single-gene transgenic with controversial public acceptance, genomic information and technology is paving the way for increasingly complex designs based in multiple gene engineering. Those designs aiming at the production of inexpensive health-promoting compounds are most likely to be welcomed by consumers. In this project we plan to develop new multigene assembling tools. During this PhD, a standardized collectio

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15

Canto, Pastor Alex. "Small RNAs in tomato : from defence to development." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/273780.

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RNA silencing is a major regulator of gene expression in plants, controlling from development to transposable element silencing and stress responses. As part of the silencing machinery, micro (mi)RNAs orchestrate silencing of their targets, either directly or through cascades of secondary small interfering (si)RNAs. To investigate the role of RNA silencing in plant immunity, I chose to focus on the miR482/2118 family, because of its diversity and presence in many plant species since the appearance of seed plants, with most genomes containing several copies, and because its members target seque

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16

Wierzba, Michael. "A Family of Four LRR-RLKs Modulate Development and Defense Signaling in Arabidopsis thaliana through Interaction with the Co-receptor BAK1." Diss., The University of Arizona, 2014. http://hdl.handle.net/10150/312668.

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Receptor-like kinases (RLKs) are encoded for by one of the largest gene families in Arabidopsis and represent the predominant form of cell surface receptors in plants. RLKs mediate signal transduction in diverse processes including steroid-mediated growth pathways, pathogen-triggered innate immune responses. Here I present characterization of mutant phenotypes, expression patterns, and genetic interactions for the BAK1 INTERACTING RECEPTOR (BIR) family of Leucine-rich Repeat-RLKs, three members of which have had no previous characterization. Furthermore, I show that cell death, aerial grow

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17

Chun, Elizabeth M. "Developing a Recombinant Plant Virus Nanoparticle Vaccine for Rift Valley Fever Virus." Scholarship @ Claremont, 2019. https://scholarship.claremont.edu/scripps_theses/1345.

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Rift Valley Fever (RVF) is an emerging infectious disease found in both livestock and humans. RVF is associated with high abortion and mortality rates in livestock and can be fatal in humans. As such, RVF is economically and socially significant to affected smallholder and subsistence farmers, those infected, and national livestock industries. However, Rift Valley Fever virus (RVFV) vaccines are not commercially available outside of endemic areas or for humans, and current vaccines are limited in their safety and efficacy. A plant-based, viral nanoparticle vaccine offers a more affordable alte

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18

Mitra, Sayantan. "Arabidopsis Cohesin proteins: WAPL, CTF7 and PHD finger proteins: MMDL1, MMDL2 are essential for proper meiosis, gamete development and plant growth." Miami University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=miami1517605898967702.

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19

Iaffaldano, Brian. "Evaluating the Development and Potential Ecological Impact of Genetically Engineered Taraxacum kok-saghyz." The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1452174223.

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20

Bustamante, Montoya Mariana. "Genomic analyses of the cup-shaped cotyledoni 1 network." Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/461296.

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El desarrollo de la flor ha sido un importante campo de investigación por muchos años y el análisis extensivo de interacciones genéticas resultó en un marco de referencia que permitió entender cómo se produce la especificación de los órganos florales. Recientemente, la introducción de tecnologías de nivel genómico permitió confrimar y expandir los modelos existentes sobre el establecimiento de la identidad de los órganos florales y otros eventos importantes que ocurren durante la formación de la flor. A pesar de esto, hay otros aspectos del desarrollo floral para los cuales no existen modelos

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21

Basu, Debarati. "Identification and Characterization of Five Arabidopsis Hydroxyproline Galactosyltransferases and Their Functional Roles in Arabinogalactan-Protein Glycosylation, Growth, Development, and Cellular Signaling." Ohio University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1438874729.

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22

Rodrigo-Peiris, Thushani. "Unraveling the Functions of Plant Ran GTPase-Activating Protein (RanGAP) by T-DNA Mutant Analysis and Investigation of Molecular Interactions of Tandem Zinc Finger 1 (TZF1) in Arabidopsis thaliana." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1343796551.

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23

Evans, Joshua R. "Transcriptional Regulation of Select Light-Harvesting Genes during Photoacclimation in Lympha mucosa gen. et sp. prov. (Batrachospermales, Rhodophyta)." Ohio University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1501068045787163.

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24

McIntosh, Cecilia A., Karin Bartoszuk, and Scott Kirkby. "Professional Development Provided by the School of Graduate Studies: Enhancing Mentoring and the Graduate Student Experience." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etsu-works/363.

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East Tennessee State University has taken several approaches to offering professional development for graduate students over the past several years. This includes graduate student research grants, thesis and dissertation awards, teaching awards, awards for service for the public good, Graduate Student Success Specialist service, Thesis/Dissertation/Capstone Boot Camp, Add-on Fellowships, GA Fee Scholarship, Thesis/Dissertation Scholarships, formal courses (GRAD), graduate student research magazine, and workshops. These will be briefly described, including funding sources supporting the initiat

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25

Brown, Jennifer Erin. "The evolutionary mechanisms promoting sex chromosome divergence within Carica papaya." Miami University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=miami1385934540.

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26

Deblais, Loic. "Understanding of Salmonella-phytopathogen-environment-plant interactions and development of novel antimicrobial to reduce the Salmonella burden in fresh tomato production." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1534437638478448.

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27

Khojayori, Farahnoz N. "Floral symmetry genes elucidate the development and evolution of oil-bee pollinated flowers of Malpighiaceae and Krameriaceae." VCU Scholars Compass, 2018. https://scholarscompass.vcu.edu/etd/5585.

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Specialization on insect and animal pollinators is thought to be the driving force for the evolution of floral traits. Specifically in the New World (NW), the oil-bee pollination syndrome has led to the convergence of floral characters in two distantly related families of core eudicots, Malpighiaceae and Krameriaceae. Both families display a flag-like structure that establishes a zygomorphic flower and floral oil rewards in epithelial elaiophores. These traits work concomitantly to attract and reward female oil-bees that help fertilize these flowers and in return receive oils. The underlying g

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28

Adhikari, Binaya. "Understanding natural expression of cytoplasmic male sterility in flowering plants using a wildflower Lobelia siphilitica L. (Campanulaceae)." Kent State University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=kent1532954470078823.

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29

Dahl, Anna Caroline E. "Membrane protein mechanotransduction : computational studies and analytics development." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:67798647-8ed5-46e0-bde9-c71235fe70ba.

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Membrane protein mechanotransduction is the altered function of an integral membrane protein in response to mechanical force. Such mechanosensors are found in all kingdoms of life, and increasing numbers of membrane proteins have been found to exhibit mechanosensitivity. How they mechanotransduce is an active research area and the topic of this thesis. The methodology employed is classical molecular dynamics (MD) simulations. MD systems are complex, and two programs were developed to reduce this apparent complexity in terms of both visual abstraction and statistical analysis. Bendix detects an

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30

Nething, Daniel B. "Detection of Cellulose Synthase Antisense Transcripts Involved in Regulating Cell Wall Biosynthesis in Barley, Brachypodium and Arabidopsis." Ohio University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1500996680467756.

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31

Greer, Mary Ruth. "PART 1: Screening of Thirty-one Medicinal Plant Species Against Herpes Simplex Virus, Acetone and Methanol Extracts from the Root Tissue of Kalanchoe pinnata Interferes with HSV Types 1 and 2 DNA replication and Early and Late Gene Expression Preventing the Spread of HSV in vitro. PART 2: Professional Development Curriculum: Integrating Molecular Biology and Microbiology into the Existing Secondary Biology Curricula." BYU ScholarsArchive, 2009. https://scholarsarchive.byu.edu/etd/6160.

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PART 1: Thirty-one medicinal plant species from Hawaii, Morocco, and the Sonoran Desert, USA have been shown in past studies to be highly inhibitory to pathogenic bacteria, fungi, and certain cancer cell lines. However, none were tested for antiviral activity. Acetone and methanol extracts from these species were bio-assayed for antiviral activity against herpes simplex virus types 1 and 2 (HSV 1 and HSV 2) and for cytotoxicity to the Vero C1008 cell line. Extracts from these species were tested in vitro for antiviral activity using an immunoperoxidase mini-plaque reduction assay to detect vir

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32

Gunadi, Andika. "Characterization of Rps8 and Rps3 Resistance Genes to Phytophthora sojae through Genetic Fine Mapping and Physical Mapping of Soybean Chromosome 13." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1354640151.

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33

Zhang, Yuan. "Functional Characterization of Beta-Glucuronosyltransferases (GLCATs) and Hydroxyproline-Galactosyltransferases (GALTs) Involved in Arabinogalactan-Protein (AGP) Glycosylation Using CRISPR/Cas9 Gene Editing Technology In Arabidopsis." Ohio University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1588687871450172.

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34

Dusi. "CYSTINE-KNOT PEPTIDES AND BBX MICROPROTEINS AS CONTROLLING FACTORS OF FLOWER AND FRUIT DEVELOPMENT." Doctoral thesis, 2022. http://hdl.handle.net/11562/1074488.

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Cystine-knot peptides are members of the large family of Cysteine-rich proteins with a dimension typically less than 50 amino acids in their mature form, characterized by the presence of six conserved cysteine (Cys) residues linked by three disulfide bonds in a knotted arrangement (Rees & Lipscomb, 1980). Peptides containing the knot motif are widespread in various species such as fungi, insects, mollusks, and mammals, where they mainly play a defense role against microorganisms, acting as toxins, or as signals in cell defense pathways (Craik et al., 2001; Iyer & Acharya, 2011; Schwarz

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35

Murphy, Phillip James. "Plant-fungal interactions during vesicular-arbuscular mycorrhiza development : a molecular approach / Phillip James Murphy." Thesis, 1995. http://hdl.handle.net/2440/18631.

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Bibliography: leaves 153-185.<br>[ix], 200, [29] leaves, [6] leaves of plates : ill. (chiefly col.) ; 30 cm.<br>Vesicular-arbuscular (VA) mycorrhiza formation is a complex process which is under the genetic control of both plant and fungus. This project aims to develop a model infection system in Hordeum vulgare L. (barley) suitable for molecular analysis; to identify host plant genes differentially expressed during the early stages of the infection process; and to screen a mutant barley population for phenotypes which form abnormal mycorrhizas.<br>Thesis (Ph.D.)--University of Adelaide, Dept.

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36

Bernabé, Orts Juan Miguel. "Development and characterization of two new tools for plant genetic engineering: A CRISPR/Cas12a-based mutagenesis system and a PhiC31-based gene switch." Doctoral thesis, 2019. http://hdl.handle.net/10251/133055.

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[ES] La mejora genética vegetal tiene como objetivo la obtención de plantas con rasgos mejorados o características novedosas que podrían ayudar a superar los objetivos de sostenibilidad. Para este fin, la biotecnología vegetal necesita incorporar nuevas herramientas de ingeniería genética que combinen una mayor precisión con una mayor capacidad de mejora. Las herramientas de edición genética recientemente descubiertas basadas en la tecnología CRISPR/Cas9 han abierto el camino para modificar los genomas de las plantas con una precisión sin precedentes. Por otro lado, los nuevos enfoques de biol

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