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1

Santos, Regineide Xavier, and Iara Pacheco Gomes Volejo. "Estudo experimental in vivo e in vitro de plantas medicinais nos processos de embriotoxicidade e teratogenicidade." Saúde e meio ambiente: revista interdisciplinar 10 (October 19, 2021): 227–40. http://dx.doi.org/10.24302/sma.v10.2797.

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Анотація:
Este trabalho tem como objetivo contribuir na compilação de artigos científicos sobre as plantas medicinais que possuem efeitos embriotóxicos e teratogênicos comprovados através de testes experimentais in vivo e in vitro. Foi realizado um levantamento nas bases de dados Medline, Pubmed, Web of Science, Cochrane, Biblioteca virtual em saúde, Lilacs e Scielo com os descritores, “Medicinal plants, embryotoxicity”; “Medicinal plants, Teratogenic effects”; “Medicinal plants, teratogenicity” e seus correspondentes em português: “Plantas medicinais, embriotoxicidade”; “Plantas medicinais, efeitos Ter
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2

Чудецкий, Антон Игоревич, Сергей Анатольевич Родин, Ирина Борисовна Кузнецова, and Сергей Сергеевич Макаров. "VACCINIUM PRAESTANS IN VITRO EX VITRO." Вестник Бурятской государственной сельскохозяйственной академии имени В. Р. Филиппова, no. 3(68) (September 28, 2022): 112–20. http://dx.doi.org/10.34655/bgsha.2022.68.3.016.

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Анотація:
В статье приведены результаты исследований по изучению влияния субстрата, обработки современным ростостимулярующими препаратами и мульчирования на процесс адаптации растений красники (Vaccinium praestans Lamb.) in vitro к нестерильным условиям ex vitro. В последнее время происходит интенсивное уменьшение запасов естественных угодий ценных видов лесных ягодных растений. Красника - малоизвестное в европейской части России лесное ягодное растение с ограниченным ареалом естественного произрастания. Для промышленного выращивания лесных ягодных растений целесообразно использовать метод микроклональн
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3

Untachai, Jeeranun, Wanwisa Dodgson, Akeapot Srifa, and Jolyon Dodgson. "In-vitro Antibacterial Activities of Selected Traditional Plants." Journal of Pure and Applied Microbiology 12, no. 1 (2018): 265–76. http://dx.doi.org/10.22207/jpam.12.1.31.

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4

Zinatullina, A. E. "MORPHOHISTOLOGICAL PECULIARITIES OF RHIZOGENESIS IN VITRO IN WHEAT EMBRYONIC CALLUSES." ÈKOBIOTEH 7, no. 1 (2023): 15–26. http://dx.doi.org/10.31163/2618-964x-2024-7-1-15-26.

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Анотація:
In modern biotechnological studies of economically valuable plants, numerous developments aimed at obtaining calluses are presented, in which various pathways and types of morphogenesis are induced in vitro. At the same time, such the type of morphogenesis as rhizogenesis in vitro, consisting in the formation and development of roots, but without further regeneration of plants, has not been studied sufficiently. Nevertheless, it is important to identify the tissue mechanisms of formation and development in calluses the roots as rhizogenesis structures. Using the example of calluses obtained fr
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5

Reuther, G. "In vitro embryogenese in plants." Scientia Horticulturae 67, no. 3-4 (1996): 249–50. http://dx.doi.org/10.1016/s0304-4238(96)00903-x.

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6

Opatrna, J. "In Vitro Embryogenesis in Plants." Biologia plantarum 38, no. 4 (1996): 616. http://dx.doi.org/10.1007/bf02890619.

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7

Salybekova, N. N., and D. Sh Fayzullaeva. "ASPECTS OF CLONALMICROPROPAGATION AND CONSERVATION OF PLANTS IN VITRO." BIOLOGICAL SCIENCES JOURNAL 1 (2023): 12–20. http://dx.doi.org/10.52081/bsj.2023.v01.i1.002.

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Анотація:
Despite more than a century of research on effective biotechnological methods to reproduce various plant species, microclonal reproduction continues to be an important tool for large- scale production. The clonal seedlings of important species maintain genetic fidelity and do not contain pests. In some cases, microclonal propagation is the only method that contributes to the maintenance and economic value of specific agricultural plant species. Microclonal reproduction as a method has solved many phytosanitary problems and has allowed both expansion and access to high-quality plants for produc
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8

AKIMOVA, S. V., V. V. KIRKACH, A. K. RADZHABOV, and G. E. TER-PETROSYANTS. "MORPHOBIOLOGICAL FEATURES OF DIAPHRAGM FORMATION IN IN VITRO AND EX VITRO GRAPE PLANTS OF INTERSPECIFIC ORIGIN." Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii, no. 6 (2021): 5–13. http://dx.doi.org/10.26897/0021-342x-2021-6-5-13.

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Анотація:
Clonal micropropagation of grape varieties of interspecific origin Moscow white (Vitis amurensis Rupr. × Vitis vinifera L.) showed that on the 40th day of the animation stage, a monopodial type of branching is typical for micro-plants, and there is no diaphragm in all nodes of micro-shoots. On the 120th day of growing in containers, the researchers revealed that the nodes of the plant shoots, from the 1st to the 6th, have a monopodial branching type. Parenchymal cells which form an incomplete diaphragm are present in the seventh node. A fully formed diaphragm appears from the eighth node, and
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9

Buyun, L. "Adaptative changes of leaf surface of tropical orchid Cattleya gaskelliana (N.E.Br.) B.S. Williams after transferring from in vitro to ex vitro conditions." Modern Phytomorphology 3 (April 1, 2013): 293–96. https://doi.org/10.5281/zenodo.162037.

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Анотація:
The leaf surface micromorphology of Cattleya gaskelliana juvenile plants, propagated in vitrofrom seeds, as well as of adult plants, cultivated in glasshouse, was analyzed by scanning electron microscopy (SEM). The leaves of both juvenile and adult plants are hypostomatic, their stomata are of tetracytic type. It was found that development of single stomata on the adaxial leaf surface of juvenile plants was induced by in vitro conditions. During the acclimation of in vitro propagated plants to glasshouse conditions the following changes of leaf surface micromorphology have been observed: 1) co
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10

Klimenko, V. P., I. A. Pavlova, V. A. Volodin, and S. M. Gorislavets. "NORMALIZATION OF GRAPE PLANTS IN VITRO FROM PHYTOPLASMA." Scientific Works of North Caucasian Federal Scientific Center of Horticulture, Viticulture, Wine-making 30 (December 2020): 112–17. http://dx.doi.org/10.30679/2587-9847-2020-30-112-117.

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11

HANDA, Takashi, and Hiroshi HARADA. "In vitro Culture of Higher Plants." Environment Control in Biology 30, no. 2 (1992): 53–58. http://dx.doi.org/10.2525/ecb1963.30.53.

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12

Rokosa, Marta, and Małgorzata Mikiciuk. "In vitro REGENERATION OF Fragraria PLANTS." Acta Scientiarum Polonorum Hortorum Cultus 16, no. 5 (2017): 145–58. http://dx.doi.org/10.24326/asphc.2017.5.15.

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13

Ibrahim, Ibrahim A., H. A. Emara, A. A. Nower, and A. Y. Abodiab. "In vitro Cultivation of Potato Plants." International Journal of Current Microbiology and Applied Sciences 5, no. 12 (2016): 858–68. http://dx.doi.org/10.20546/ijcmas.2016.512.094.

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14

Corrêa da Silva, Diogo Pedrosa, Elif Aylin Ozudogru, Michele Valquíria Dos Reis, and Maurizio Lambardi. "In vitro conservation of ornamental plants." Ornamental Horticulture 24, no. 1 (2018): 28–33. http://dx.doi.org/10.14295/oh.v24i1.1163.

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Анотація:
The market of flowers and ornamental plants is dependent on the diversification of species and the availability of high quality propagation materials. Actually, in vitro culture techniques performance a prominent role in the multiplication and maintenance of commercially propagated ornamental plant species, and are promising for the production of thousands of high quality plants in relatively short term. In addition, when market demand for a particular species is low or zero in a specific period of the year, in vitro culture techniques allow the conservation of cultures under aseptic condition
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15

Atanassov, A., N. Zagorska, P. Boyadjiev, and D. Djilianov. "In vitro production of haploid plants." World Journal of Microbiology & Biotechnology 11, no. 4 (1995): 400–408. http://dx.doi.org/10.1007/bf00364615.

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16

Geetha, S., D. Padmanabhan, W. Wilfred Manuel, and A. Ayyamperumal. "In Vitro Production of Sugarcane Plants." Sugar Tech 2, no. 3 (2000): 47–48. http://dx.doi.org/10.1007/bf02945757.

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17

DeWald, M. G., G. A. Moore, W. B. Sherman, and M. H. Evans. "Production of pineapple plants in vitro." Plant Cell Reports 7, no. 7 (1988): 535–37. http://dx.doi.org/10.1007/bf00272750.

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18

Brown Jr, R. M., L. Li, K. Okuda, et al. "In Vitro Cellulose Synthesis in Plants." Plant Physiology 105, no. 1 (1994): 1–2. http://dx.doi.org/10.1104/pp.105.1.1.

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19

Strzelczyk-Żyta, Danuta. "In Vitro Preservation of Potato Plants." Plant Breeding and Seed Science 76, no. 1 (2017): 69–73. http://dx.doi.org/10.1515/plass-2017-0024.

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Анотація:
Abstract Potato plants free of viruses, bacteria and viroid could be maintained in vitro for a long time. Proper preparation of potato plants for in vitro culture provide its long-term storage in good condition. First step is to establish in vitro culture from young greenhouse grown plants in early developmental stage. Explants are maintained and propagated by nodal subculture on hormone-free Murashige and Skoog (MS) medium at temperature 20-22°C. After rooting, for longer preservation plants are maintained at temperature 8-10°C.
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20

Debergh, P. C. "In vitro culture of higher plants." Scientia Horticulturae 38, no. 3-4 (1989): 315–16. http://dx.doi.org/10.1016/0304-4238(89)90079-4.

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21

Harborne, Jeffrey B. "In vitro culture of higher plants:." Phytochemistry 27, no. 9 (1988): 3033. http://dx.doi.org/10.1016/0031-9422(88)80728-3.

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22

Doroshenko, Natalya, and Valentina Puzyrnova. "RIPENING PLANTS TO CREATE A GRAPES COLLECTION IN VITRO." Bulletin of KSAU, no. 11 (February 6, 2025): 10–18. https://doi.org/10.36718/1819-4036-2023-11-10-18.

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Анотація:
The purpose of the study is to develop a method for creating a collection of the grape gene pool in vitro using the combined use of biotechnological methods: modification of the nutrient medium and cultivation of plants under conditions of low positive temperature. During the cultivation process with an increased content of sucrose in the nutrient medium (50−70 g/l), autumn color of the leaves appears and shoots ripen in various grape varieties: Sypun Cherniy, Vierul, Tsimlyansky Cherniy (clone 2–3), Cabernet Sauvignon, Kumshatsky, Rupestris du Lo, Tsimladar, less in varieties Krasnostop zolot
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23

Oksana, Chornobrov, and Tkachova Olena. "IN VIVO ADAPTATION OF REGENERANT PLANTS OF FRAGARIA VESCA L. CULTIVARS." Plant varieties studying and protection 16, no. 3 (2020): 248–52. https://doi.org/10.21498/2518-1017.16.3.2020.214925.

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Анотація:
<strong>Purpose</strong>. The adaptation of regenerant plants to environmental conditions is the final stage of micropropagation. According to a number of authors, when in vitro plants are transferred to in vivo non-sterile conditions, a significant percentage of mortality is recorded. In a previous publication, the regenerative capacity of strawberry (Fragaria vesca L.) in vitro tissues on MS culture medium (Murashige &amp; Skoog, 1962) and a regenerants was obtained (Chornobrov O. Yu., 2019). <strong>The objective of the study</strong>&nbsp;is to develop an optimal protocol of acclimation of
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24

Santos Gonçalves, Douglas, Denys Matheus Santana Costa Souza, Letícia Vaz Molinari, et al. "CLONAL MICROPLANT PRODUCTION, MORPHOLOGICAL EVALUATION AND GENETIC STABILITY OF Dendrocalamus asper (Schult. & Schult.) Backer ex. K. Heyneke." Nativa 11, no. 1 (2023): 01–09. http://dx.doi.org/10.31413/nativa.v11i1.14394.

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ABSTRACT: Bamboo species have many commercial applications, considering that homogeneous plantations (formed from clonal plants) are essential to high sustainable biomass production. The cloning of selected plants on an industrial scale through in vitro cultivation has many advantages, being important for the supply of plants in sufficient quantity and quality to meet commercial demand. The control of the cloning is the basis for an industrial scale, and its knowledge can optimize the process. This work aimed to evaluate the cloning of Dendrocalamus asper selected plant through micropropagatio
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25

Makarov, Sergey, Irina Kuznecova, Hasyan Sharafutdinov, Anton Chudeckiy, Liliya Ahmetova, and Andrey Kul'chickiy. "EVALUATION OF THE INFLUENCE OF VARIOUS FACTORS ON IN VITRO ROOTING AND EX VITRO ADAPTATION OF RUSSIAN CULTIVARS OF PRUNUS CERASUS L." Bulletin of KSAU, no. 11 (February 6, 2025): 121–29. https://doi.org/10.36718/1819-4036-2023-11-121-129.

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The purpose of research is to study the characteristics of rhizogenesis in vitro of domestic varieties of P. cerasus and their adaptation to non-sterile conditions ex vitro. The study was carried out on the basis of the Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, Kostroma State Agricultural Academy and Northern Federal University named after M.V. Lomonosov using generally accepted methods for clonal micropropagation of plants. The objects of the study were P. cerasus plants of two Russian varieties of medium ripening – Novella and Shokoladnitsa, which are in gre
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26

Babanina, Svetlana, Natalya Egorova, Olga Yakimova, and Mariya Kovalenko. "ADAPTATION TO EX VITRO CONDITIONS OF MICROPLANTS LAVANDULA ANGUSTIFOLIA MILL. AT LONG-TERM REPRODUCTION IN VITRO." Vestnik of Kazan State Agrarian University 18, no. 3 (2023): 11–19. http://dx.doi.org/10.12737/2073-0462-2023-11-19.

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The purpose of the study is to identify the features of adaptation to ex vitro conditions of lavender plants after long-term clonal micropropagation. The experiments were carried out on microplants of narrow-leaved lavender (Lavandula angustifolia Mill.), cv. The number of plants in each subcultivation - n=10 pcs., 3-fold repetition. Microplants with well-developed shoots and roots were planted in a mixture of peat and perlite (1:1) and grown at illumination of 2–3 klx, photoperiod duration of 16 h, temperature of 24 ± 2°C, air humidity of 70%. The frequency of adaptation of microplants, depen
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27

Twardovska, M. O., I. I. Konvalyuk, K. V. Lystvan, I. O. Andreev, and V. A. Kunakh. "The content of phenolic compounds and flavonoids in in vitro plants and tissue culture of Deschampsia antarctica E. Desv." Faktori eksperimental'noi evolucii organizmiv 26 (September 1, 2020): 276–81. http://dx.doi.org/10.7124/feeo.v26.1279.

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Анотація:
Aim. The aim of the study was a comparative assessment of total phenolic content and total flavonoid content in in vitro plants, regenerated plants, plants grown in a growth chamber, and tissue culture of several genotypes of Deschampsia antarctica. Methods. In vitro culture, Folin-Ciocalteu method, spectrophotometry, high-performance liquid chromatography. Results. The total content of phenolic compounds and total flavonoid content was determined in the samples of three D. antarctica genotypes: G/D12-2a (2n=26), DAR12 (2n=26+0–3B) and Y66 (2n=36–39). The content of these biologically active c
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28

C S Ranasinghe, L K Weerakoon, Y M H Liyanage, and D T Mathes. "PHYSIOLOGICAL ASPECTS OF IN VITRO-GROWN COCONUT (Cocos nucifera L.) PLANTS DURING ACCLIMATIZATION." CORD 15, no. 02 (1999): 34. http://dx.doi.org/10.37833/cord.v15i02.332.

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The physiological status of in vitro-grown coconut (Cocos nucifera L.) plants during acclimatization was studied using nursery-raised seedlings as the control. The percentage of open stomata in leaves of in vitro-grown coconut plants was high at the initial stage of acclimatization but decreased during the course of acclimatization indicating an improvement in stomatal regulation. A progressive increase in the stomatal density, epicuticular wax deposition and leaf thickness in in vitro-grown plants was observed during acclimatization. As a result of the low light intensity, the epidermal cells
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29

Yeo, Mohamed Anderson, Dan Gbongue Lucien Gogbeu, Navigué Abou Yeo, Tanoh Hilaire Kouakou, and Lacina Coulibaly. "Morphological and agronomical characterization of pineapple plants derived from vitro culture." International Journal of Current Research in Biosciences and Plant Biology 9, no. 5 (2022): 21–28. http://dx.doi.org/10.20546/ijcrbp.2022.905.002.

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In Côte d'Ivoire, pineapple occupies a prominent place after the coffee and cocoa binomial in exports. The country has long been the leading producer of smooth Cayenne in the European market. However, this dynamism is hampered by the lack of good quality rejects. To overcome this constraint, various methods of in vitro regeneration of plantlets have been initiated. Micro propagation and somatic embryogenesis allow massive production of plants of good sanitary quality. However, acclimatization of the vitro plants is a critical phase. This study was conducted to assess the growth parameters and
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30

Nas, Mehmet Nuri, Nedim Mutlu, and Paul E. Read. "Random Amplified Polymorphic DNA (RAPD) Analysis of Long-term Cultured Hybrid Hazelnut." HortScience 39, no. 5 (2004): 1079–82. http://dx.doi.org/10.21273/hortsci.39.5.1079.

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RAPD and phenotypic analysis were conducted to assess clonal stability of hazelnuts generated from axillary buds cultured in vitro for long-term. The nuts produced on in vitro-propagated plants were indistinguishable from those of donor plants. With the exception of rare horizontal (plagiotropic) growth, all in vitro-propagated plants exhibited phenotypes similar to those of donor plants. RAPD analysis did not reveal any somaclonal variation between donor plants from which in vitro cultures were initiated and micropropagated plants (6-year cultures), and no somaclonal variation was detected am
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31

Slavtcheva, T., and V. Dimitrova. "Gas Exchange of In Vitro and Ex Vitro Grown Grapevine Plants." Photosynthetica 39, no. 1 (2001): 29–33. http://dx.doi.org/10.1023/a:1012431516297.

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32

Lubell-Brand, Jessica D., Lauren E. Kurtz, and Mark H. Brand. "An In Vitro–Ex Vitro Micropropagation System for Hemp." HortTechnology 31, no. 2 (2021): 199–207. http://dx.doi.org/10.21273/horttech04779-20.

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Hyperhydricity of shoots initiated in vitro, poor shoot extension, inability of shoot cultures to maintain good growth over an extended time, and unsuccessful ex vitro rooting have limited the development of a commercial scale micropropagation system for hemp (Cannabis sativa). We present a culture initiation method that prevents shoot hyperhydricity using vented-lid vessels with 0.2-µm pores and medium containing agar at 1% (w/v). To optimize shoot multiplication in vitro, a control medium (medium A) and four treatment media (medium B, C, D, and E), with varying inorganic nutrients and vitami
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33

Pacheco, Georgia, Rachel Fátima Gagliardi, Mariana Buturini Cogliatti, et al. "Influence of substrates and in vitro preconditioning treatments on ex vitro acclimatization of Arachis retusa." Pesquisa Agropecuária Brasileira 41, no. 1 (2006): 165–69. http://dx.doi.org/10.1590/s0100-204x2006000100023.

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Анотація:
The objective of this work was to evaluate the influence of substrate and preconditioning treatments on the acclimatization of in vitro plants of Arachis retusa. Plants were transferred to Plantmax or sand, and fertilized with Hoagland's nutrient solution. Plants maintained in sand, with or without fertilizer, showed the highest survival rates. In order to evaluate the influence of in vitro preconditioning treatments, stem segments were cultured on MS medium supplemented with different sucrose concentrations. The highest survival and developmental rates were observed in plants from two accessi
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34

Маyorova, О. Yu. "ADAPTATION OF THE OBTAINED in vitro Gentiana lutea L. PLANTS TO ex vitro AND in situ CONDITIONS." Biotechnologia Acta 8, no. 6 (2015): 77–86. http://dx.doi.org/10.15407/biotech8.06.077.

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35

Smith, M. A. L., M. T. McClelland, and R. Timmermann. "Anomalous Root Structure on Woody Plants in vitro." Journal of Environmental Horticulture 9, no. 2 (1991): 61–64. http://dx.doi.org/10.24266/0738-2898-9.2.61.

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Анотація:
Abstract The anatomical structure of in vitro-generated roots contrasted sharply with ex vitro roots produced on parallel treatments of micropropagated woody plant clones. In vitro roots contained enlarged, irregular, frequently pigmented cortical cells with numerous intercellular spaces, and only primary vascular system 4–5 weeks after initiation, while ex vitro roots of the same age were comprised of smaller, uniformly arranged cell layers and were developmentally further advanced (exhibited secondary vascular system development). Light exposure was not a major influence on anomalous structu
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36

Al-Khayri, J. M., F. H. Huang, and T. E. Morelock. "IN VITRO FLOWERING OF REGENERATED SPINACH PLANTS." HortScience 25, no. 9 (1990): 1121d—1121. http://dx.doi.org/10.21273/hortsci.25.9.1121d.

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Regenerated spinach (Spinacia oleracea L.) maintained under a 10-h photoperiod (65 uE m-2 s-1) after an incubation period on a GA-containing medium were induced to flower in vitro. The plantlets were regenerated from callus initiated on MS medium with 2.0 mg L-1 kinetin and 0.5 mg L-1 2,4-D and were subsequently transferred to a medium containing 2.0 mg L-1 kinetin, 1.0 mg L-1 GA, and 0.01 mg L-1 2-4,D. While on the regeneration medium, the cultures were exposed to a long-day photoperiod. Regenerants were transferred to an IBA-containing medium for rooting, after which flowering was observed.
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37

DEBERGH, Pierre C. "ACCLIMATIZATION TECHNIQUES OF PLANTS FROM IN VITRO." Acta Horticulturae, no. 289 (April 1991): 291–300. http://dx.doi.org/10.17660/actahortic.1991.289.77.

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38

Phillips, Gregory C. "In vitro morphogenesis in plants-recent advances." In Vitro Cellular & Developmental Biology - Plant 40, no. 4 (2004): 342–45. http://dx.doi.org/10.1079/ivp2004555.

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39

Charles, G., L. Rossignol, and M. Rossignol. "Environmental Effects on Potato Plants in vitro." Journal of Plant Physiology 139, no. 6 (1992): 708–13. http://dx.doi.org/10.1016/s0176-1617(11)81715-3.

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40

Calderón, A. I., C. Terreaux, M. P. Gupta, and K. Hostettmann. "In vitro cytotoxicity of 11 Panamanian plants." Fitoterapia 74, no. 4 (2003): 378–83. http://dx.doi.org/10.1016/s0367-326x(03)00052-2.

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41

Smyda-Dajmund, Paulina. "Virus Elimination from In Vitro Potato Plants." Plant Breeding and Seed Science 76, no. 1 (2017): 81–85. http://dx.doi.org/10.1515/plass-2017-0026.

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Abstract The routine and the most effective method of viruses elimination from potato plants is in vitro culture of apical shoots combined with thermo- and/or chemotherapy. At times electrotherapy or cryotherapy is also applied. Elimination of potato viruses by thermotherapy required treatment of infected plants with high temperature (35°C - 45°C) for about two weeks. Thermotherapy is useful for elimination of PLRV and PVY from potato plants. In chemotherapy antimetabolite, like ribavirin is used. Chemotherapy is dedicated for elimination of PVM, PVS and PVX. Very often the successful virus el
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42

Mat Taha, R., N. A. Hasbullah, and A. Awal. "IN VITRO FLOWERING OF SELECTED ORNAMENTAL PLANTS." Acta Horticulturae, no. 881 (November 2010): 141–46. http://dx.doi.org/10.17660/actahortic.2010.881.15.

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43

Saúco, Víctor Galán, and John Charles Robinson. "Field establishment ofin vitro-produced banana plants." Fruits 65, no. 1 (2010): 43–51. http://dx.doi.org/10.1051/fruits/2009041.

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44

Robinson, John Charles, and Victor Galán Sáuco. "Weaning (acclimatization) ofin vitro-produced banana plants." Fruits 64, no. 5 (2009): 325–32. http://dx.doi.org/10.1051/fruits:2009026.

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45

Robinson, John Charles, and Victor Galán Sáuco. "Nursery hardening ofin vitro-produced banana plants." Fruits 64, no. 6 (2009): 383–92. http://dx.doi.org/10.1051/fruits:2009034.

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46

Pant, Hem Chandra, Naveen Gaurav, and Harsh Vardhan Pant. "Mountainous therapeutic plant Nardostachys jatamansi (D.Don) DC grown in vitro used as a substitute for wild plants." Journal of Drug Research in Ayurvedic Sciences 9, no. 5 (2024): 335–46. https://doi.org/10.4103/jdras.jdras_87_24.

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Abstract BACKGROUND: Nardostachys jatamansi is widely used in the Unani, Ayurveda, and Siddha medicine systems. The extensive use of this plant in traditional medicine makes it an endemic plant. N. Jatamansi relieves symptoms of anxiety, epilepsy and weak memory, whereas plant oil (basically known as spikenard oil) is used to maintain hair growth, cardiac problems, and diabetes. METHODS: In our study, we tried to validate in vitro-grown plants as a substitute for wild-type plants for therapeutic purposes. We compared the phytochemical and biological (antibacterial) activity of in vitro-grown a
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47

Tsygankova, Victoria A., Yaroslav V. Andrusevich, Elena N. Shysha, et al. "RNAi-mediated Resistance against Plant Parasitic Nematodes of Wheat Plants Obtained in Vitro Using Bioregulators of Microbiological Origin." Current Chemical Biology 13, no. 1 (2019): 73–89. http://dx.doi.org/10.2174/2212796812666180507130017.

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Background:Plant parasitic nematodes are dangerous pests that damage various agricultural crops and decrease their productivity.Objective:The resistance of new lines of wheat (Triticum aestivum L.) plants obtained under in vitro conditions on MS media containing microbial bioregulators to plant parasitic nematodes was studied under in vitro and greenhouse conditions.Methods:Here we conducted physiological and molecular-genetic studies of resistance of wheat plants to nematodes.Results:In vitro experiments showed that wheat plants grown on MS media with microbial bioregulators had 20-37 % of in
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48

Gonçalves, José Carlos, Maria Teresa Coelho, Maria da Graça Diogo, et al. "In vitro Shoot Cultures of Pterospartum tridentatum as an Alternative to Wild Plants as a Source of Bioactive Compounds." Natural Product Communications 13, no. 4 (2018): 1934578X1801300. http://dx.doi.org/10.1177/1934578x1801300415.

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The aim of this study was to evaluate the composition of aqueous extracts of in vitro shoot cultures and wild plants of Pterospartum tridentatum in order to promote the use of this plant material as a possible source of bioactive compounds. The extraction yield from wild plants compared with in vitro shoot cultures was lower. The total phenolic contents of in vitro shoots were significantly lower compared with those of wild plants. The phenolic profiles of in vitro shoots were very similar to those of wild plants, regardless of the source. However, taxifolin-6- C-glucoside, as well as rutin an
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49

Hrytsak, L. R., and N. M. Drobyk. "Modern technologies of increasing the tolerance of in vitro cultured plants to ex vitro conditions." Faktori eksperimental'noi evolucii organizmiv 26 (September 1, 2020): 183–89. http://dx.doi.org/10.7124/feeo.v26.1347.

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Aim. To analyze the experience of Ukrainian and foreign scientists on technologies to increase the adaptive potential of cultivated in vitro plants to ex vitro conditions. Results. Modern acclimatization technologies are mainly aimed at improving the methods of adaptation of planting material of in vitro collections to ex vitro conditions. Much less attention is paid to technologies to increase plant resilience at the stage of their multiplication and growth in vitro. Integration and systematization of research results of a large number of scientists is allowed to describe the main strategies
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50

Shelepova, Olga V., Tatyana A. Dilovarova, Alexander A. Gulevich, et al. "Chemical Components and Biological Activities of Essential Oils of Mentha × piperita L. from Field-Grown and Field-Acclimated after In Vitro Propagation Plants." Agronomy 11, no. 11 (2021): 2314. http://dx.doi.org/10.3390/agronomy11112314.

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In this work, we studied in vitro propagation of three cultivars of Mentha × piperita L. Murashige and Skoog medium (MS) supplemented with 0.5 mg·L−1 BAP was the most optimal medium for micropropagation of the cultivars studied. The ability of peppermint plants field-acclimated after in vitro micropropagation to produce essential oils (EOs) was investigated. EO was obtained by hydrodistillation from dried leaves and flowering shoots from control (field grown) plants and plants acclimated in field conditions after in vitro propagation. The samples were collected at the first and second year of
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