Готові списки джерел за темами / Polyamine spermidine

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Добірка наукової літератури з теми "Polyamine spermidine"

Автор: Grafiati

Опубліковано: 10 січня 2023

Оновлено: 28 січня 2023

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Статті в журналах з теми "Polyamine spermidine"

Amana, Koei H., and Shigeru Matsuzaki. "Polyamines and their biosynthetic activities in nonphytopathogenic marine agrobacteria." Canadian Journal of Microbiology 36, no. 8 (August 1, 1990): 567–72. http://dx.doi.org/10.1139/m90-099.

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Eight strains of nonphytopathogenic agrobacteria, whose taxonomic positions are uncertain, were analyzed for their polyamine contents and their polyamine biosynthetic activities. They were separated into five types on the basis of polyamine distribution patterns. The first group contains putrescine, cadaverine, and spermidine as major polyamines, the second spermidine alone, the third cadaverine alone, the fourth putrescine, spermidine, spermine, homospermidine, and aminopropyl-homospermidine, and the last contains trace amounts of diaminopropane and spermidine. These polyamine patterns of eight species seem to suggest no close phylogenic relationship among these agrobacteria and are different from those of other typical, phytopathogenic species belonging to Agrobacterium. Key words: agrobacteria, decarboxylases, polyamines, homospermidine, aminopropylhomospermidine.

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Chibucos, M. Constantine, and Paul F. Morris. "Levels of Polyamines and Kinetic Characterization of Their Uptake in the Soybean Pathogen Phytophthora sojae." Applied and Environmental Microbiology 72, no. 5 (May 2006): 3350–56. http://dx.doi.org/10.1128/aem.72.5.3350-3356.2006.

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ABSTRACT Polyamines are ubiquitous biologically active aliphatic cations that are at least transiently available in the soil from decaying organic matter. Our objectives in this study were to characterize polyamine uptake kinetics in Phytophthora sojae zoospores and to quantify endogenous polyamines in hyphae, zoospores, and soybean roots. Zoospores contained 10 times more free putrescine than spermidine, while hyphae contained only 4 times as much free putrescine as spermidine. Zoospores contained no conjugated putrescine, but conjugated spermidine was present. Hyphae contained both conjugated putrescine and spermidine at levels comparable to the hyphal free putrescine and spermidine levels. In soybean roots, cadaverine was the most abundant polyamine, but only putrescine efflux was detected. The selective efflux of putrescine suggests that the regulation of polyamine availability is part of the overall plant strategy to influence microbial growth in the rhizosphere. In zoospores, uptake experiments with [1,4-14C]putrescine and [1,4-14C]spermidine confirmed the existence of high-affinity polyamine transport for both polyamines. Putrescine uptake was reduced by high levels of exogenous spermidine, but spermidine uptake was not reduced by exogenous putrescine. These observations suggest that P. sojae zoospores express at least two high-affinity polyamine transporters, one that is spermidine specific and a second that is putrescine specific or putrescine preferential. Disruption of polyamine uptake or metabolism has major effects on a wide range of cellular activities in other organisms and has been proposed as a potential control strategy for Phytophthora. Inhibition of polyamine uptake may be a means of reducing the fitness of the zoospore along with subsequent developmental stages that precede infection.

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Hamana, Koei, and Shigeru Matsuzaki. "Polyamine distribution patterns serve as a phenotypic marker in the chemotaxonomy of the Proteobacteria." Canadian Journal of Microbiology 39, no. 3 (March 1, 1993): 304–10. http://dx.doi.org/10.1139/m93-043.

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Polyamines of various genera of the class Proteobacteria were analyzed by high-performance liquid chromatography to determine if they can serve as taxonomic markers. The major polyamine of Zymomonas was homospermidine, whereas the Acetobacter–Gluconobacter complex contained spermidine, suggesting the presence of two different polyamine distribution patterns in the alpha subclass. Both the homospermidine-dominant type and the spermidine-dominant type were found in heterogeneous Sphingomonas species. Typical species belonging to the gamma subclass have their own unique polyamine pattern in Xanthomonas (spermidine), Azomonas (putrescine), Frateuria (spermidine), Alteromonas (putrescine–spermidine or spermidine), Shewanella (putrescine), Marinomonas (putrescine–spermidine or spermidine), Halomonas (putrescine–spermidine or spermidine), and Deleya (spermidine). Cadaverine was sporadically distributed in some species in these genera. Some strains classified into Rhizobacter, Zoogloea, Azomonas, or Alteromonas contained 2-hydroxyputrescine found exclusively in the beta subclass. Polyamine distribution patterns are genus- and (or) species-specific and can serve as a phenotypic marker in the chemotaxonomy of the Proteobacteria.Key words: polyamine, chemotaxonomy, Proteobacteria.

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Madeo, Frank, Sebastian J. Hofer, Tobias Pendl, Maria A. Bauer, Tobias Eisenberg, Didac Carmona-Gutierrez, and Guido Kroemer. "Nutritional Aspects of Spermidine." Annual Review of Nutrition 40, no. 1 (September 23, 2020): 135–59. http://dx.doi.org/10.1146/annurev-nutr-120419-015419.

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Анотація:

Natural polyamines (spermidine and spermine) are small, positively charged molecules that are ubiquitously found within organisms and cells. They exert numerous (intra)cellular functions and have been implicated to protect against several age-related diseases. Although polyamine levels decline in a complex age-dependent, tissue-, and cell type–specific manner, they are maintained in healthy nonagenarians and centenarians. Increased polyamine levels, including through enhanced dietary intake, have been consistently linked to improved health and reduced overall mortality. In preclinical models, dietary supplementation with spermidine prolongs life span and health span. In this review, we highlight salient aspects of nutritional polyamine intake and summarize the current knowledge of organismal and cellular uptake and distribution of dietary (and gastrointestinal) polyamines and their impact on human health. We further summarize clinical and epidemiological studies of dietary polyamines.

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Davis, Rohan A., Daniela Vullo, Claudiu T. Supuran, and Sally-Ann Poulsen. "Natural Product Polyamines That Inhibit Human Carbonic Anhydrases." BioMed Research International 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/374079.

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Natural product compound collections have proven an effective way to access chemical diversity and recent findings have identified phenolic, coumarin, and polyamine natural products as atypical chemotypes that inhibit carbonic anhydrases (CAs). CA enzymes are implicated as targets of variable drug therapeutic classes and the discovery of selective, drug-like CA inhibitors is essential. Just two natural product polyamines, spermine and spermidine, have until now been investigated as CA inhibitors. In this study, five more complex natural product polyamines1–5, derived from either marine sponge or fungi, were considered for inhibition of six different human CA isozymes of interest in therapeutic drug development. All compounds share a simple polyamine core fragment, either spermine or spermidine, yet display substantially different structure activity relationships for CA inhibition. Notably, polyamines1–5were submicromolar inhibitors of the cancer drug target CA IX, this is more potent than either spermine or spermidine.

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Kramer, George F., Chien Yi Wang, and William S. Conway. "Inhibition of Softening by Polyamine Application in `Golden Delicious' and `McIntosh' Apples." Journal of the American Society for Horticultural Science 116, no. 5 (September 1991): 813–17. http://dx.doi.org/10.21273/jashs.116.5.813.

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Pressure infiltration of `Golden Delicious' and `McIntosh' apples (Malus domestica Borkh.) with polyamides resulted in an immediate increase in firmness. `Golden Delicious' apples were 2.7 N (0.25 mM spermidine) to 6.7 N (1.0 mM spermine) firmer, while `McIntosh' apples were 2.2 N (0.25 mM spermidine) to 5.3 N (1.0 mM spermine) firmer than the water-treated control. During 28 weeks of storage at 0C, the differences between the polyamine-treated and water-treated apples were even larger. Similar results were observed with a 3% Ca treatment, but the Ca treatment reduced the rate of softening to a greater extent than did the polyamine treatments in `Golden Delicious'. Polyamides increased the endogenous levels of the polyamides infiltrated; however, the levels declined rapidly with time in storage. Both polyamine and Ca inhibited the development of chilling injury symptoms (brown core) in `McIntosh'. The influence of polyamines on ethylene production was negligible in both cultivars. The Ca treatment, however, inhibited ethylene evolution in `Golden Delicious'. Polyamides, thus, may affect apple softening through rigidification of cell walls rather than through interactions with ethylene metabolism.

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Murray-Stewart, Tracy, Matthew Dunworth, Jackson Foley, Charles Schwartz, and Robert Casero. "Polyamine Homeostasis in Snyder-Robinson Syndrome." Medical Sciences 6, no. 4 (December 7, 2018): 112. http://dx.doi.org/10.3390/medsci6040112.

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Loss-of-function mutations of the spermine synthase gene (SMS) result in Snyder-Robinson Syndrome (SRS), a recessive X-linked syndrome characterized by intellectual disability, osteoporosis, hypotonia, speech abnormalities, kyphoscoliosis, and seizures. As SMS catalyzes the biosynthesis of the polyamine spermine from its precursor spermidine, SMS deficiency causes a lack of spermine with an accumulation of spermidine. As polyamines, spermine, and spermidine play essential cellular roles that require tight homeostatic control to ensure normal cell growth, differentiation, and survival. Using patient-derived lymphoblast cell lines, we sought to comprehensively investigate the effects of SMS deficiency on polyamine homeostatic mechanisms including polyamine biosynthetic and catabolic enzymes, derivatives of the natural polyamines, and polyamine transport activity. In addition to decreased spermine and increased spermidine in SRS cells, ornithine decarboxylase activity and its product putrescine were significantly decreased. Treatment of SRS cells with exogenous spermine revealed that polyamine transport was active, as the cells accumulated spermine, decreased their spermidine level, and established a spermidine-to-spermine ratio within the range of wildtype cells. SRS cells also demonstrated elevated levels of tissue transglutaminase, a change associated with certain neurodegenerative diseases. These studies form a basis for further investigations into the leading biochemical changes and properties of SMS-mutant cells that potentially represent therapeutic targets for the treatment of Snyder-Robinson Syndrome.

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Olle, H. "Putrescine, Spermidine, and Spermine." Physiology 1, no. 1 (February 1, 1986): 12–15. http://dx.doi.org/10.1152/physiologyonline.1986.1.1.12.

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Putrescine was first isolated from putrifying meat and was thought of as a decomposition product;spermine was named from its occurrence in semen. These polyamines, however, are now known to have important roles in cell growth and differentiation. Their physiological significance can be studied by analyzing the consequences of depletion of the cellular polyamine content. The results include arrest of cell growth, differentiation, and division. Further results suggest that blocking of polyamine synthesis may have broad implications in clinical medicine.

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Erwin, B. G., and A. E. Pegg. "Regulation of spermidine/spermine N1-acetyltransferase in L6 cells by polyamines and related compounds." Biochemical Journal 238, no. 2 (September 1, 1986): 581–87. http://dx.doi.org/10.1042/bj2380581.

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Exposure of rat L6 cells in culture to exogenous polyamines led to a very large increase in the activity of spermidine/spermine N1-acetyltransferase. Spermine was more potent than spermidine in bringing about this increase, but in both cases the elevated acetyltransferase activity increased the cellular conversion of spermidine into putrescine. The N1-acetyltransferase turned over very rapidly in the L6 cells, with a half-life of 9 min after spermidine and 18 min after spermine. A wide variety of synthetic polyamine analogues also brought about a substantial induction of spermidine/spermine N1-acetyltransferase activity. These included sym-norspermidine, sym-norspermine, sym-homospermidine, N4-substituted spermidine derivatives, 1,3,6-triaminohexane, 1,4,7-triaminoheptane and deoxyspergualin, which were comparable with spermidine in their potency, and N1N8-bis(ethyl)spermidine, N1N9-bis(ethyl)homospermidine, methylglyoxal bis(guanylhydrazone), ethylglyoxal bis(guanylhydrazone) and 1,1′-[(methylethanediylidene)dinitrilo]bis(3-amino-guanidine), which were even more active than spermidine. It is suggested that these polyamine analogues may bring about a decrease in cellular polyamines not only by inhibiting biosynthesis but by stimulating the degradation of spermidine into putrescine.

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Morgan, D. M. L. "Oxidized polyamines and the growth of human vascular endothelial cells. Prevention of cytotoxic effects by selective acetylation." Biochemical Journal 242, no. 2 (March 1, 1987): 347–52. http://dx.doi.org/10.1042/bj2420347.

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The responses of human umbilical-vein vascular endothelial cells in culture to the naturally occurring polyamines spermine, spermidine and putrescine, their acetyl derivatives and oxidation products were examined. In the absence of human polyamine oxidase, exposure of cells to polyamines (up to 160 microM) had no adverse effects. In the presence of polyamine oxidase, spermine and spermidine were cytotoxic, but putrescine was not. Acetylation of the aminopropyl group of spermidine or both aminopropyl groups of spermine prevented this cytotoxicity. The amino acids corresponding to the polyamines, representing a further stage of oxidation, were also without effect. The cytotoxic effects were irreversible. Use of bovine serum amine oxidase in place of the human enzyme gave qualitatively similar results.

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Більше джерел

Дисертації з теми "Polyamine spermidine"

Catros, Véronique. "Implication des polyamines dans les processus proliferatifs malins." Rennes 1, 1990. http://www.theses.fr/1990REN1T085.

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Des etudes effectuees in vivo chez l'animal ainsi que chez des patients atteints de differents types histologiques de tumeurs, ont permis de montrer que le taux erythrocytaire de spermidine (spd) se comportait comme un index sanguin de la proliferation cellulaire tumorale. L'etude in vivo du metabolisme des polyamines (pa) apres administration de precurseurs radiomarques a des animaux porteurs de tumeurs greffees a permis d'apporter la preuve de l'origine tumorale de la spermidine erythrocytaire. Les modalites d'uptake des pa par les erythrocytes ont par ailleurs ete caracterisees in vitro: les erythrocytes de souris cancereuses presentent des modifications de leurs proteines stromales, leur conferant la capacite de capter trois fois plus de #1#4c spd que les erythrocytes de souris saines. A l'inverse de ce qui se passe au cours d'un processus proliferatif controle, ou l'evolution des taux erythrocytaires de pa est parfaitement regulee, en cas de cancer, les pa s'accumulent dans les hematies. Cette accumulation est correlee a une reduction de la concentration tissulaire en malonaldehyde (mda), une molecule inhibitrice de la replication de l'adn. L'etude du catabolisme des pa dans les cellules cancereuses ainsi que l'utilisation de drogues modulatrices de ce metabolisme permettent d'evoquer l'hypothese d'un role des pa erythrocytaires dans le controle homeostatique de la proliferation cellulaire. Les effets biologiques obtenus avec des analogues tetramethyles de pa permettent egalement d'envisager l'utilisation du metabolisme des pa a des fins therapeutiques et diagnostiques en cancerologie

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Chen, Alina. "New polyamine analogues as potential antineoplastic agents." Scholarly Commons, 2000. https://scholarlycommons.pacific.edu/uop_etds/2680.

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The naturally occurring polyamines play an essential role in cell growth and proliferation. The levels of polyamines have been shown to increase in rapidly proliferating cancer cells. Therefore, compounds that inhibit enzymes in polyamine biosynthetic pathway may have therapeutic potential. Compounds capable of providing both in vitro and in vivo inhibition of almost all enzymes in the polyamine biosynthetic pathway are known. An exception is the lack of an agent that inhibits spermidine/spermine N 1 -acetyltransferase (SSAT), the rate-limiting enzyme in the catabolism of polyamines. The design, synthesis and characterization of five new polyamine analogues as potential inhibitors of SSAT are presented. Three compounds, N 1 -[3-(propenamido) propyl]-1,4-diaminobutane dihydrochloride 5 , N 1 -[3-(maleimido)propyl]-1,4-diamino-butane dihydrochloride 7 and N 1 -[3-(2-bromoacetamido)propyl]-1,4-diaminobutane dihydrochloride 9 , were designed as active-site-directed affinity label inhibitors. Two compounds, N-[N-(5-acetamido-2-hydroxypentyl-3-aminopropyl)]-1,4-diaminobutane trihydrochloride 12 and N-[3-(2-hydroxyethylamino)propyl]-1,4-diaminobutane trihydrochloride 14 , were designed as transition state-like analogue inhibitors. These compounds were synthesized using one key intermediate, N-(3-aminopropyl)-N,N ′ -bis-(tert-butoxycarbonyl)-1,4-diaminobutane 3 . Three of these synthesized compounds, 5 , 7 and 12 were evaluated for their ability to inhibit SSAT. The enzyme used was a crude extract of human large cell undifferentiated lung carcinoma cell line NCI H157 cells. These synthetic analogues when tested against the crude enzyme extract at concentrations of 0.05, 0.1, 1 and 5 μM appeared to show no effects on the activity of SSAT.

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Vaishali, Mulangi Gopala Reddy. "Characterization of Polyamine Transporters from Rice and Arabidopsis." Bowling Green State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1303231265.

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Li, Jun. "A study of the role of spermidine/spermine N¹-acetyltransferase (SSAT) in polyamine homeostasis in human prostate cancer cells." Thesis, University of Aberdeen, 2014. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=210088.

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Prostate cancer is the second leading cancer in men. A large amount of polyamines are synthesised in the human prostate and are involved in prostate cell growth and its physiological functions. The content of intracellular polyamines is closely related to cell growth. An increase in cell growth is accompanied by a rise of intracellular polyamine content, and a depletion of intracellular polyamine pools can cause growth arrest or cell death. Therefore, maintaining polyamine concentrations is critical to the cell. Spermidine/spermine N1-acetyltransferase (SSAT) is the first and rate-limiting enzyme in the polyamine catabolic pathway. SSAT gene is highly inducible, with many stimuli including polyamine analogues and some anticancer drugs producing dramatic increases in activity. Many studies have focussed on polyamine analogues as inducers of SSAT activity as increases in SSAT are associated with a growth inhibition in many tumour cells. However, the mechanisms of this inhibition are not fully understood with respect to polyamine content. Additionally, in vivo results in SSAT transgenic mice studies are contradictory. For example, prostate carcinogenesis is reduced in TRAMP mice but Apcmin/+ mice show a promoted intestinal tumorigenesis. It is thus necessary to characterise the regulation of polyamine content and metabolism by SSAT in prostate cancer cells. The aim of the present study was to characterise the role of SSAT in both the growth of LNCaP prostate carcinoma cells and the response of these cells to anticancer drugs. Our hypothesis is that increased SSAT activity will inhibit cell growth and that this is associated with a decrease of intracellular polyamine pools. Furthermore, if SSAT induction is an essential part of the response of cancer cells to anticancer drugs, then altered SSAT activity should affect sensitivity of the cells to the drugs. The present study used a cell culture model of human prostate cancer: LNCaP wild type (WT) and SSAT cDNA transfected prostate carcinoma cell lines. The expression of SSAT in the transfected cell line (SSAT- & SSAT+) was controlled through the “Tet-off” system. This model system provided a background for comparison of effects under basal (WT), low (SSAT-), and high (SSAT+) SSAT activity. Due to our interest in acetylpolyamine derivatives and their low concentrations in cells, a new method for quantifying polyamine concentrations was developed using liquid chromatography-mass spectrometry (LC-MS). This method was highly sensitive and can detect polyamines about 250 fold lower than HPLC, as well as N-acetylpolyamines and N1,N12-diacetylspermine. In addition, a variety of methods were utilised to measure cell growth, enzyme activity, protein expression, polyamine efflux and apoptosis, which includes enzyme assays, western blot, radiochemical labelled assays, flow cytometry, spectrophotometry and fluorescent microscopy. A stable increase in SSAT activity was inhibitory to the cell growth. This inhibition was associated with significant changes in the activity of the polyamine pathway. The alterations included an increase in ODC, APAO, and SMO activity; an accumulation of intracellular N1-acetylspermidine and putrescine; a decrease in intracellular spermidine and spermine; an increased polyamine flux and efflux; and an increase in apoptosis. Combination treatment to the cells with DFMO and MDL72527 partially restored the growth of SSAT+ cells. The original contribution of this study to the field is that the cells with a higher SSAT activity are less sensitive to aspirin and 5-FU, and the sensitivity increased while the overexpressed SSAT activity decreased. The growth inhibition was associated with a depletion of total intracellular polyamine pools by the drug treatments. Moreover, to our knowledge, it is first time that the extracellular polyamine concentrations were quantified by LC-MS in human tumour cells. Overall, an increase in SSAT activity led to an inhibition of prostate cancer cell growth, and vice versa. Thereby, this study suggests that SSAT is a potential target for novel drug discovery for cancer chemotherapy or chemoprevention. For example, a combination treatment could be designed that acts as an inducer of SSAT activity in tumour cells, leading to an inhibition of the cell growth in the first place and increased sensitivity to cytotoxic agents. This would then be followed by an agent to decrease SSAT activity when the sensitivity of cancer cells to the cytotoxic treatment was optimal.

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Ariyaratne, Menaka M. "A new perspective on polyamine biosynthesis and transport in arabidopsis thaliana." Bowling Green State University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1555693507751475.

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Fredericks, Eugene B. (Eugene Bernard). ""Studies involving alterations of polyamine metabolism in Arabidopsis thaliana"." Monash University, Dept. of Biological Sciences, 2001. http://arrow.monash.edu.au/hdl/1959.1/8432.

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AUBEL, CORINNE. "Effet d'une carence en acide amine sur deux mecanismes majeurs de l'homeostasie des polyamines : le transport membranaire et le catabolisme par la spermidine/spermine n1-acetyltransferase (doctorat)." Clermont-Ferrand 1, 2001. http://www.theses.fr/2001CLF1MM13.

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Rioux, Benjamin. "Synthèse et vectorisation de biomolécules type Chalcone en vue d'une application anticancéreuse." Thesis, Limoges, 2016. http://www.theses.fr/2016LIMO0104/document.

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La synthèse et la vectorisation d’agents anticancéreux constituent des axes de recherche majeurs du LCSN. De nombreux composés naturels possèdent des propriétés anticancéreuses, mais ils sont abandonnés en raison de leur manque de sélectivité vis-à-vis des cellules cancéreuses ou de leur faible biodisponibilité. Ainsi, un grand intérêt est actuellement porté sur le développement de médicaments spécifiquement vectorisés vers les cellules cancéreuses. Les vecteurs utilisés dans ce travail sont des dérivés de polyamines et des nano objets de type β-cyclodextrines / nanocristaux de cellulose (β-CD/CNCx). Les polyamines vont permettre un ciblage actif des cellules cancéreuses grâce au système de transport de polyamine (PTS) surexprimé dans ces cellules. Les nano objets vont cibler spécifiquement les tumeurs via un ciblage passif dû à l’effet EPR. Les principes actifs employés dans cette étude sont des flavonoïdes, et plus particulièrement des chalcones. En effet, les flavonoïdes, qui constituent une large famille de composés phénoliques naturels, sont connus pour leurs nombreux effets biologiques comme les activités antioxydantes, anti-inflammatoires et anti-prolifératives.L’intérêt du LCSN à la fois pour les chalcones et les agents anticancéreux nous a conduits à concevoir de nouveaux composés antiprolifératifs vectorisés. Ce travail présente dans un premier temps la synthèse de chalcones et l’obtention de dérivés couplés aux différents vecteurs décrits précédemment (motifs polyaminés,β-CD/CNCx) ; un travail sur la synthèse d’une bis-chalcone via le couplage de Suzuki est également exposé.L’ensemble des molécules obtenues est caractérisé par des analyses RMN 1H, 13C et HRMS. Dans une seconde partie, nous présentons l’ensemble des évaluations biologiques des composés précédemment obtenus. Ces évaluations sont réalisées par un test de viabilité cellulaire (test MTT) sur quatre lignées cancéreuses : deux colorectales (HT-29 et HCT-116) et deux prostatiques (PC-3 et DU-145)
Synthesis and vectorization of anticancer agents are major research themes of LCSN. Many natural compoundspossess anti-cancer properties, but they are dropped because of their lack of selectivity to cancer cells or theirlow bioavailability. Thus, great interest is currently focused on the development of drugs specifically vectorizedto cancer cells. The vectors used in this work are polyamine derivatives and nano-objects type Î²-cyclodextrin /cellulose nanocrystals (Î²-CD/CNCx). Polyamines allow active targeting of cancer cells through the polyaminetransport system (PTS) overexpressed in these cells. Nano-objects specifically target tumors using a passivetargeting due to the EPR effect. Drugs used in this study are flavonoids, especially chalcones. Indeed,flavonoids, which constitute a large family of natural phenolic compounds, are known for their numerousbiological effects such as antioxidant, anti-inflammatory and anti-proliferative activities. The interest of LCSNfor both chalcones and anticancer agents led us to design new vectorized anti-proliferative compounds. Firstly,this work shows the synthesis of chalcones and their derivatives coupled to various above-described vectors(polyamines units, Î²-CD/CNCx); a work on the synthesis of a bis-chalcone through the Suzuki coupling reactionis also exposed. All molecules obtained are characterized by 1H NMR, 13C NMR and HRMS analysis. In thesecond part of this work, we present all biological evaluations of compounds previously obtained. Theseassessments are performed through a cell viability test (MTT test) on four cancer cell lines: two colorectal (HT-29 and HCT-116) and two prostate (PC-3 and DU-145) cell lines

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Chiteri, Kevin Oyale. "Functional & Phylogenetic Analysis of Arabidopsis thaliana Organic Cation Transporters (OCT5 & OCT1) Genes in Polyamine Transport in Plants." Bowling Green State University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1563038129138996.

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Ménard, Florian. "Benzoporphyrines polyaminées et glycolisées : synthèses et tests biologiques." Limoges, 2008. https://aurore.unilim.fr/theses/nxfile/default/8fa60a2e-636b-4c5d-b022-4d474de42c60/blobholder:0/2008LIMO4065.pdf.

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Ce travail porte sur la synthèse multi-étapes, la caractérisation et l'évaluation biologique de nouveaux photosensibilisateurs vectorisés conçus pour une utilisation potentielle en photothérapie dynamique des cancers. Nous avons élaboré plusieurs tétrabenzoporphyrines symétriques substituées par quatre polyamines ou quatre molécules de glucose, dans le but d’augmenter leur sélectivité pour les cellules cancéreuses. Ces dérivés de benzoporphyrines ont été obtenus suivant deux approches distinctes. Ainsi, la fixation des vecteurs (spermidine, spermine, α-D-glucose ou β-D-glucose) a été effectuée soit en fin de synthèse, directement sur le précurseur macrocyclique, soit dans la phase initiale, sur le synthon aldéhydique. Tous les produits finaux obtenus ont révélé leur aptitude à produire de l’oxygène singulet en présence de lumière visible ; finalement, des tests de viabilité cellulaire ont été réalisés, in vitro, sur deux lignées cancéreuses humaines (MCF-7 et HaCaT)
This work reports the multi-step synthesis, characterization and biological evaluation of new vectorized photosensitizers designed for their potential use in phototherapy of cancers. This series of molecules consisted in symmetrical tetrabenzoporphyrins to which four glucosyl or polyamine units have been attached in order to increase their selectivity for cancer cells. These benzoporphyrin derivatives were obtained through two distinct approaches: attachment of the vectors (spermidine, spermine, α-D-glucose or β-D-glucose) has been carried out either at the very end of synthesis, on the macrocyclic precursor, or during the initial step, on the aldehydic synthon. All of these final products have proved their ability to produce singlet oxygen in the presence of visible light, and their effects on cell viability have been tested, in vitro, on two human cancer cell lines (MCF-7 and HaCaT)

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Книги з теми "Polyamine spermidine"

Tower, Paula Allene. Homospermidine, spermidine, and putrescine: The biosynthesis and metabolism of polyamines in Rhizobium meliloti. 1987.

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Частини книг з теми "Polyamine spermidine"

Perin, Antonio, Angela Sessa, and M. Alfonsina Desiderio. "Estrogenic Control of Spermidine/Spermine N1-Acetyltransferase Activity in Rat Uterus." In Progress in Polyamine Research, 345–51. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5637-0_30.

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Sunkara, Prasad S., John H. Zwolshen, Nellikunja J. Prakash, and Terry L. Bowlin. "Mechanism of Antitumor Activity of Norspermidine, a Structural Homologue of Spermidine." In Progress in Polyamine Research, 707–16. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5637-0_62.

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Canellakis, Zoe Nakos. "Spermidine in Mammalian Lymphocytes and Sea Urchin Embryos: Uptake and Labeling of Macromolecules." In Progress in Polyamine Research, 423–34. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5637-0_37.

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Porter, Carl W., and Raymond J. Bergeron. "Regulation of Polyamine Biosynthetic Activity by Spermidine and Spermine Analogs — A Novel Antiproliferative Strategy." In Progress in Polyamine Research, 677–90. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5637-0_60.

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Wolff, Edith C., and Myung Hee Park. "Role of the Polyamine Spermidine as a Precursor for Hypusine Modification in eIF5A." In Polyamines, 121–29. Tokyo: Springer Japan, 2014. http://dx.doi.org/10.1007/978-4-431-55212-3_10.

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Fairlamb, Alan H. "The Role of Glutathionylspermidine and Trypanothione in Regulation of Intracellular Spermidine Levels During Growth of Crithidia Fasciculata." In Progress in Polyamine Research, 667–74. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5637-0_59.

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Porta, R., C. Esposito, S. Metafora, A. Malorni, P. Pucci, and G. Marino. "Purification and Structural Characterization of In Vitro Synthesized (γ-Glutamyl) Spermidine Conjugates of a Major Protein Secreted from the Rat Seminal Vesicles." In Progress in Polyamine Research, 403–9. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5637-0_35.

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Moschou, Panagiotis N. "Determination of di−/Polyamine Oxidase Activity in Plants by an In-Gel Spermidine Oxidation Assay." In Methods in Molecular Biology, 141–47. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7398-9_14.

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Feirer, R. P., S. R. Wann, and D. W. Einspahr. "The effects of spermidine synthesis inhibitors on in-vitro plant development." In Polyamines in Plants, 117–25. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5171-6_10.

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Rips, R., and C. Guette. "Determination of Polyamines (Spermine, Spermidine, Putrescine) in Biological Samples." In Contemporary Electroanalytical Chemistry, 299–302. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4899-3704-9_33.

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Тези доповідей конференцій з теми "Polyamine spermidine"

Kuo, Be-Sheng, Gil Korner, and Thorir D. Bjornsson. "ROLE OF POLYAMINES IN THE REGULATION OF SYNTHESIS AND SECRETION OF PLASMINOGEN ACTIVATOR FROM BOVINE AORTIC ENDOTHELIAL CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644655.

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The effects of three polyamines, putrescine (PUT), spermidine (SPD) and spermine (SPM), were investigated on the synthesis and secretion of plasminogen activator (PA) and antiactivator (PAI) activities in confluent bovine aortic endothelial cells. PA activity was determined bythe fibrin plate method, and individual species with PA and PAI activities were separated and visualized using SDS-PAGE with zymography and reverse fibrin autography. Both control cells and cells treated with polyamines secreted PA activity in a time-dependent fashion. After 24-hour incubation, the three polyamines enhanced PA secretion in a dose-dependent manner (10-6 to 2.5 × 10-3 M), with a potency order of SPM > SPD> PUT, as estimated by the fibrin plate method. The maximum PA releases after PUT (0.5 mM), SPD (2.5 mM) and SPM (0.5 mM) were 1.7, 4.5 and 5.4 times control levels, respectively. Concentrations lower than 1 μM had essentially no effects. The enhancement of PA activity by polyamines was blocked by actino-mycin D and cycloheximide, while it was not affected by inhibitors of polyamine biosynthesis except that the enhancement by PUT (0.5 mM) was reduced by methylglyoxal bis(guanylhydrazone). These data suggest that polyamines directly stimulate PA synthesis and secretion through promotion of gene transcription and translation, and that this effect appears to be related to their position in the biosynthetic pathway of polyamines. The kinetic patterns of activities of ornithine decarboxylase and S-adenosy-methionine decarboxylase in confluent endothelial cells stimulated withfresh culture medium suggest that there is rapid turnover of intracellular polyamines. Multiple forms of secreted PA were observed and both tissue- and urokinase-type PA were enhanced by polyamines, while the PAI activity, as evaluted by reverse fibrin autograpy, was apparently reduced. These experimental results suggest that polyamines may play an important role in the regulation of the synthesis and secretion of plasminogen activators, and that this biological function could be modified by disease states and by agents that are associated with altered polyamine metabolism.

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Lu, Jun, Mingming Li, Hong Xu, Malcolm Tingle, and Garth J. S. Cooper. "Abstract LB-287: Spermidine/spermine actyltransferase is responsible for the metabolism of triethylenetetramine and other polyamine analogs in humans." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-lb-287.

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