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Готові списки джерел за темами / Proteins Synthesis

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Добірка наукової літератури з теми "Proteins Synthesis"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 30 липня 2024

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Статті в журналах з теми "Proteins Synthesis"

1

Nilsson, Bradley L., Matthew B. Soellner, and Ronald T. Raines. "Chemical Synthesis of Proteins." Annual Review of Biophysics and Biomolecular Structure 34, no. 1 (June 2005): 91–118. http://dx.doi.org/10.1146/annurev.biophys.34.040204.144700.

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2

Gibney, Brian R., Francesc Rabanal, and P. Leslie Dutton. "Synthesis of novel proteins." Current Opinion in Chemical Biology 1, no. 4 (December 1997): 537–42. http://dx.doi.org/10.1016/s1367-5931(97)80050-6.

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3

Mejuch, Tom, and Herbert Waldmann. "Synthesis of Lipidated Proteins." Bioconjugate Chemistry 27, no. 8 (August 2016): 1771–83. http://dx.doi.org/10.1021/acs.bioconjchem.6b00261.

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4

Borgia, Jeffrey A., and Gregg B. Fields. "Chemical synthesis of proteins." Trends in Biotechnology 18, no. 6 (June 2000): 243–51. http://dx.doi.org/10.1016/s0167-7799(00)01445-1.

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5

Hilvert, Donald. "Chemical synthesis of proteins." Chemistry & Biology 1, no. 4 (December 1994): 201–3. http://dx.doi.org/10.1016/1074-5521(94)90011-6.

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6

Plocinski, P., N. Arora, K. Sarva, E. Blaszczyk, H. Qin, N. Das, R. Plocinska, et al. "Mycobacterium tuberculosis CwsA Interacts with CrgA and Wag31, and the CrgA-CwsA Complex Is Involved in Peptidoglycan Synthesis and Cell Shape Determination." Journal of Bacteriology 194, no. 23 (September 21, 2012): 6398–409. http://dx.doi.org/10.1128/jb.01005-12.

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ABSTRACTBacterial cell division and cell wall synthesis are highly coordinated processes involving multiple proteins. Here, we show that Rv0008c, a novel small membrane protein fromMycobacterium tuberculosis, localizes to the poles and on membranes and shows an overall punctate localization throughout the cell. Furthermore, Rv0008c interacts with two proteins, CrgA and Wag31, implicated in peptidoglycan (PG) synthesis in mycobacteria. Deletion of the Rv0008c homolog inM. smegmatis, MSMEG_0023, caused bulged cell poles, formation of rounded cells, and defects in polar localization of Wag31 and

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7

Kohen, Amnon, Priyanka Singh, and Qi Guo. "Chemoenzymatic Synthesis of Ubiquitous Biological Redox Cofactors." Synlett 28, no. 10 (April 10, 2017): 1151–59. http://dx.doi.org/10.1055/s-0036-1588768.

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Redox cofactors are utilized by a myriad of proteins, ranging from metabolic enzymes to those performing post-translational modifications. Labeled redox cofactors have served as a vital tool for a broad range of studies. This account describes chemoenzymatic syntheses of the isotopically labeled, biologically important redox cofactors: nicotinamide adenine dinucleotide, methylene tetrahydrofolate, and flavin nucleotides. An overview of the general strategy is presented. These examples demonstrate the utility of enzymatic synthesis.1 Introduction2 Nicotinamide Cofactors2.1 Synthesis of Remote-L

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8

Metanis, Norman, Reem Mousa, and Post Reddy. "Chemical Protein Synthesis through Selenocysteine Chemistry." Synlett 28, no. 12 (March 21, 2017): 1389–93. http://dx.doi.org/10.1055/s-0036-1588762.

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Анотація:

Methods for the preparation of small-to-medium-sized proteins by chemical protein synthesis have matured in recent years and proven valuable for protein science. Thanks to the many recent discoveries and developments in the field, proteins up to 300 amino acids can now be prepared in the lab in a matter of days. This technology gives the scientists the flexibility to substitute any atom in the protein sequence; hence synthesis is not constrained to the 20 canonical amino acids. In this Synpacts article we briefly highlight the recent studies on selenocysteine chemistry in the field of chemical

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9

Whyte, Lyle G., and William E. Inniss. "Cold shock proteins and cold acclimation proteins in a psychrotrophic bacterium." Canadian Journal of Microbiology 38, no. 12 (December 1, 1992): 1281–85. http://dx.doi.org/10.1139/m92-211.

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The synthesis of proteins in the psychrotrophic bacterium Bacillus psychrophilus in response to both cold shock and continuous growth at low temperatures was examined. Cold shocks of 20 to 0, 5, or 10 °C resulted in the induction of nine, seven, and five cold shock proteins, respectively, as determined by 2-dimensional gel electrophoresis and computing scanning laser densitometry. Two cold shock proteins, with molecular masses of 61 and 34 kDa, which were induced in B. psychrophilus by cold shocks of 20 to 0 or 5 °C, were not induced in a cold-sensitive mutant of B. psychrophilus. Analysis of

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10

Kent, Stephen B. H. "Total chemical synthesis of proteins." Chem. Soc. Rev. 38, no. 2 (2009): 338–51. http://dx.doi.org/10.1039/b700141j.

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Дисертації з теми "Proteins Synthesis"

1

Baas, Tracey Lynn. "The design, synthesis, and characterization of template assembled synthetic proteins /." Thesis, Connect to this title online; UW restricted, 2000. http://hdl.handle.net/1773/11561.

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2

Draffan, Lynda Catherine. "Chemical synthesis of proteins." Thesis, University of Edinburgh, 1996. http://hdl.handle.net/1842/13715.

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3

Morton, Gail Helen. "The chemical synthesis of proteins." Thesis, University of Edinburgh, 1997. http://hdl.handle.net/1842/15437.

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The viability of extending the present methodology designed for the solid phase synthesis of peptides has been investigated. Using the catalytic domain of stromelysin (SCD. 173 residues) as the model system, a number of different factors affecting the preparation and purification of chemically synthesised proteins are examined. The purification SCD, prepared using stepwise solid phase synthesis is described. Following characterisation, it is evident that protein of the correct primary sequence has been prepared, furthermore, preliminary studies centred on the enzymatic activity of SCD indicate

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4

Walker, Douglas Gordon. "Characterization of immediate-early and early proteins of murine cytomegalovirus synthesized in permissive and nonpermissive cells." Thesis, University of British Columbia, 1985. http://hdl.handle.net/2429/25985.

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The gene products produced by murine cytomegalovirus (MCMV) in infected cells prior to viral DNA synthesis are believed to control the interaction of the virus with the cells, determining whether a permissive infection results, with virus replication, or whether further virus gene expression is inhibited, resulting in a latent or abortive infection. The aim of this study was to characterize the early viral gene products that are produced in permissive and nonpermissive cells. The proteins produced in 3T3-L1 cells, permissively infected with MCMV, during the first six hours of infection (the p

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5

Scott, Felicia Yi Xia. "Controlled Hybrid Material Synthesis using Synthetic Biology." Diss., Virginia Tech, 2017. http://hdl.handle.net/10919/86147.

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The concept of creating a hybrid material is motivated by the development of an improved product with acquired properties by amalgamation of components with specific desirable traits. These new attributes can range from improvements upon existing properties, such as strength and durability, to the acquisition of new abilities, such as magnetism and conductivity. Currently, the concept of an organic-inorganic hybrid material typically describes the integration of an inorganic polymer with organically derived proteins. By building on this idea and applying the advanced technologies available tod

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6

Schwartz, Anne. "Characterization of normal and androgen resistant-genital skin fibroblasts using high-resolution two-dimensional gel electrophoresis." Thesis, McGill University, 1985. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=63378.

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7

Zhang, Yinfeng, and 张银凤. "Protein chemical synthesis by serine and threonine ligation." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/202359.

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Анотація:

Landmark advances in the field of synthetic protein chemistry have enabled the preparation of complex, homogeneous proteins, including those that carry specific posttranslational modifications (PTMs). In addition, chemical synthesis will allow one to incorporate unnatural elements to generate new biologics with altered properties and functions. Native chemical ligation (NCL) is a milestone in the chemical synthesis of proteins (Kent et al., Science, 1994, 266, 776-779), in which a C-terminal peptide thioester and an N-terminal cysteine (Cys)-containing peptide-both in side-chain unprotected fo

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8

Johnston, Julie Catherine. "In vitro translation of cucumber necrosis virus RNA." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/28969.

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The in vitro translation products directed by cucumber necrosis virus (CNV) RNA were analyzed in both rabbit reticulocyte lysate and wheat germ extract cell-free translation systems. In rabbit reticulocyte lysates, one major protein of ca. 33 Mr was produced. In wheat germ extracts, four proteins of ca. 41, 33, 21 and 20 Mr were produced. Hybrid-arrested translation (HART) studies using synthetic CNV antisense RNA corresponding to the entire CNV genome demonstrated that the four major proteins synthesized from CNV virion RNA in wheat germ extracts are virus-specific translation products. The g

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9

Sun, Xiaojiao. "High Affinity Synthetic Molecular Binders for Proteins : Design, Synthesis and Evaluation." Doctoral thesis, Uppsala universitet, Fysikalisk-organisk kemi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-183203.

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This thesis describes the design and synthesis of small molecule derivatives and their polypeptide conjugates as high affinity binders for proteins: the D-dimer protein (D-dimer), a biomarker for diagnosis of thromboembolic diseases; human myeloperoxidase (MPO), a biomarker for cardiovascular diseases; and chitinases, potential targets for asthma therapy. The interactions between the synthetic binder molecules and those proteins were evaluated by surface plasmon resonance (SPR) biosensor analysis and fluorescence spectroscopy. Competition SPR experiments or other methods proved that the small

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10

Kim, Daniel. "Characterization of the Mata pre-." Scholarly Commons, 2009. https://scholarlycommons.pacific.edu/uop_etds/738.

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Книги з теми "Proteins Synthesis"

1

E, Esterhouse Toma, and Petrinos Lado B, eds. Protein biosynthesis. New York: Nova Science, 2008.

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2

1958-, Martin Robin, ed. Protein synthesis: Methods and protocols. Totowa, N.J: Humana Press, 1998.

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3

Fernando, Albericio, and Giralt Ernest, eds. Chemical approaches to the synthesis of peptides and proteins. Boca Raton: CRC Press, 1997.

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4

A, Wallace Carmichael J., ed. Protein engineering by semisynthesis. Boca Raton, Fla: CRC Press, 2000.

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5

S, Spirin A., and Swartz James R, eds. Cell-free protein synthesis: Methods and protocols. Weinheim: Wiley-VCH, 2008.

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6

G, Spedding, ed. Ribosomes and protein synthesis: A practical approach. Oxford [England]: IRL Press at Oxford University Press, 1990.

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7

L, Hatfield Dolph, Lee Byeong J, and Pirtle Robert M, eds. Transfer RNA in protein synthesis. Boca Raton: CRC Press, 1992.

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8

1939-, Bermek E., ed. Mechanisms of protein synthesis: Structure-function relations, control mechanisms, and evolutionary aspects : Proceedings of the Symposium on Molecular Mechanisms in Protein Synthesis held at Beyaz Köşk, Emigran, Bosphorus, Istanbul. Berlin: Springer-Verlag, 1985.

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9

Arnold, Revzin, ed. The Biology of nonspecific DNA-protein interactions. Boca Raton, Fla: CRC Press, 1990.

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10

Hengge-Aronis, Regine. Studies of secretion of periplasmic proteins in Escherichia coli. Konstanz: Hartung-Gorre, 1986.

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Частини книг з теми "Proteins Synthesis"

1

Buxbaum, Engelbert. "Synthesis of Peptides." In Biophysical Chemistry of Proteins, 289–90. Boston, MA: Springer US, 2010. http://dx.doi.org/10.1007/978-1-4419-7251-4_31.

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2

Kermode, Allison R., and J. Derek Bewley. "Synthesis, processing and deposition of seed proteins: The pathway of protein synthesis and deposition in the cell." In Seed Proteins, 807–41. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4431-5_34.

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3

Houghten, Richard A., Sarah R. Hoffmann, Mairead K. Bray, Nicole Frizzell, J. M. Ostresh, Suzanne M. Pratt, and John Sitarik. "Simultaneous Multiple Peptide Synthesis: The Rapid Preparation of Large Numbers of Peptides." In Proteins, 463–70. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4613-1787-6_46.

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4

Rivier, J., R. Galyean, W. Woo, D. Karr, T. Richmond, and J. Spiess. "The Symposium Test Peptide (STP): Synthesis and Characterization of a Model Peptide." In Proteins, 789–95. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4613-1787-6_81.

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5

Liu, Lei, Sam Danishefsky, and David Crich. "Chemical Synthesis of Proteins." In Organic Chemistry - Breakthroughs and Perspectives, 221–45. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2012. http://dx.doi.org/10.1002/9783527664801.ch6.

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6

Frigerio, Lorenzo, and Lynne M. Roberts. "The Synthesis of Ricinus communis Lectins." In Toxic Plant Proteins, 191–205. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-12176-0_10.

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7

Buntru, Matthias, Simon Vogel, Ricarda Finnern, and Stefan Schillberg. "Plant-Based Cell-Free Transcription and Translation of Recombinant Proteins." In Recombinant Proteins in Plants, 113–24. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2241-4_8.

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AbstractPlant cell-free lysates contain all the cellular components of the protein biosynthesis machinery, providing an alternative to intact plant cells, tissues, and whole plants for the production of recombinant proteins. Cell-free lysates achieve rapid protein production (within hours or days) and allow the synthesis of proteins that are cytotoxic or unstable in living cells. The open nature of cell-free lysates and their homogeneous and reproducible performance is ideal for protein production, especially for screening applications, allowing the direct addition of nucleic acid templates encoding proteins of interest, as well as other components such as enzyme substrates, chaperones, artificial amino acids, or labeling molecules. Here we describe procedures for the production of recombinant proteins in the ALiCE (Almost Living Cell-free Expression) system, a lysate derived from tobacco cell suspension cultures that can be used to manufacture protein products for molecular and biochemical analysis as well as applications in the pharmaceutical industry.

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8

Tanoue, Eiichiro. "Proteins in the Sea — Synthesis." In Dynamics and Characterization of Marine Organic Matter, 383–463. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-1319-1_18.

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9

Fernández-Tejada, Alberto, John Brailsford, Qiang Zhang, Jae-Hung Shieh, Malcolm A. S. Moore, and Samuel J. Danishefsky. "Total Synthesis of Glycosylated Proteins." In Protein Ligation and Total Synthesis I, 1–26. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/128_2014_622.

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10

Halvorson, Harlyn O. "The Induced Synthesis of Proteins." In Advances in Enzymology - and Related Areas of Molecular Biology, 99–156. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470122679.ch3.

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Тези доповідей конференцій з теми "Proteins Synthesis"

1

Terwilliger, Thomas C., and Joel Berendzen. "Protein Crystallography: From X-ray diffraction spots to a three-dimensional image." In Signal Recovery and Synthesis. Washington, D.C.: Optica Publishing Group, 1998. http://dx.doi.org/10.1364/srs.1998.swa.1.

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Proteins are remarkable molecular machines that are essential for life. They can do many things ranging from the precise control of blood clotting to synthesizing complex organic compounds. Pictures of protein molecules are in high demand in biotechnology because they are important for applications such as drug discovery and for engineering enzymes for commercial use.

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2

Jackson, C. W., N. K. Hutson, and S. A. Steward. "CHANGES IN PROTEIN SYNTHESIS PROFILES OF MEGAKARYOCYTES (MK) DURING MATURATION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643545.

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Several key differentiation events occur within the recognizable MK compartment; however, little is known about the macromolecular changes responsible for these events. In this study, protein synthesis profiles of morphologically immature and mature guinea pig MK populations have been analyzed by twodimensional gel electrophoresis after in vivo labeling with 35S-methionine. MK were enriched by a bovine plasma aggregation enrichment procedure (Blood 69:173, 1987) and then fractionated into immature and mature populations based on differences in their respective buoyant densities (Brit. J. Haema

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3

Kornilov, F. D., E. A. Ananiev, E. V. Vasilieva, K. S. Mineev, S. A. Goncharuk, and M. V. Goncharuk. "CO-TRANSLATIONAL FOLDING OF MEMBRANE PROTEINS DURING CELL FREE SYNTHESIS." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-337.

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The biochemical obtaining of natively folded membrane proteins (MPs) is an extremely important task of modern structural biology. We have studied the efficiency of co-translational incorporation of MPs into various membrane-mimicking media — liposomes, micelles, and bicelles during the cell-free synthesis. The retinal protein of Exiguobacterium sibiricum (ESR) was chosen as an object of study.

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4

Garcia, A., G. Liman, K. Fluke, S. Davidson, T. Santangelo, G. Chadwick, and P. Welander. "Identification of H-Gdgt Synthesis Proteins." In IMOG 2023. European Association of Geoscientists & Engineers, 2023. http://dx.doi.org/10.3997/2214-4609.202333298.

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5

Mattern-Schain, Samuel I., Mary-Anne Nguyen, Tayler M. Schimel, James Manuel, Joshua Maraj, Donald Leo, Eric Freeman, Scott Lenaghan, and Stephen A. Sarles. "Totipotent Cellularly-Inspired Materials." In ASME 2019 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/smasis2019-5745.

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Abstract This work draws inspiration from totipotent cellular systems to design smart materials whose compositions and properties can be learned or evolved. Totipotency refers to the inherent genetic potential of a single cell to adapt and produce all types of differentiated cells within an organism. To study this principal and apply it synthetically, tissue-like compartmentalized assemblies are constructed via lipid membrane-separated aqueous droplets in a hydrophobic medium through the droplet interface bilayer (DIB) method. Within our droplets, we explore synthetic totipotency via cell-free

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6

Agard, David A., Yashushi Hiraoka, and John W. Sedat. "Three-dimensional Optical Microscopy of Biological Specimens." In Signal Recovery and Synthesis. Washington, D.C.: Optica Publishing Group, 1986. http://dx.doi.org/10.1364/srs.1986.thd1.

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The ability to analyze biological specimens in three dimensions represents one of the major achievements of modern structural biology. For all but the simplest repeating structures, three-dimensional analysis is a crucial prerequisite for understanding complex biological assemblies. Near atomic resolution analysis of of crystalline proteins, nucleic acids, and viruses by X-ray crystallography approaches the routine. The current frontier focuses on the three dimensional analysis of non-crystalline, non-symmetric biological structures of cellular dimension. Electron microscope tomography and thr

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7

Rao, Jiajia. "Tuning plant protein for improved functionality and flavor profile: From field to application." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/xqxj4886.

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Recently, plant proteins are gaining in popularity as consumers are looking to rebalance their diet with more plant-based options. As such, there is a need to understand the potential of these plant proteins to deliver nutrition and functionality in various food products. However, plant proteins are still under-utilization in food and beverage industry due to their characteristic beany and off flavor, lower technical functionality, such as limited solubility, and some negative taste attributes. In general, plant protein can be obtained from pulses, cereals and oilseeds. Each plant has a unique

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8

Waart, P. v. d., K. T. Preissner, U. Delvos, and G. Müller-Berghaus. "SIMULTANEOUS PRODUCTION OF ENDOTHELIAL CELL-DERIVED PROTEIN S AND FACTOR V, AND INACTIVATION OF FACTOR Va AT THE ENDOTHELIAL CELL SURFACE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644737.

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Several proteins synthesized and expressed by endothelial cells are involved in the regulation of coagulation. The synthesis and expression of factor V and protein S has been demonstrated in independent studies. The present work evaluates the simultaneous synthesis and expression of bovine factor V and protein S and the effect of endothelial protein S on the inactivation of endothelial factor Va by activated protein C. The accumulation of both proteins in conditioned medium was detected by SDS-PAGE followed by immunoblotting, and their activities were tested by functional assays. The synthesis

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9

TAM, James P. "Design and Synthesis of Peptide Biologics by Deconstruction of Proteins." In The Twenty-Third American and the Sixth International Peptide Symposium. Prompt Scientific Publishing, 2013. http://dx.doi.org/10.17952/23aps.2013.001.

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Macmillan, Derek, and Alison M. Daines. "SEMI-SYNTHESIS OF GLYCOSYLATED PROTEINS AND MIMICS USING CHEMOSELECTIVE LIGATIONS." In XXIst International Carbohydrate Symposium 2002. TheScientificWorld Ltd, 2002. http://dx.doi.org/10.1100/tsw.2002.438.

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Звіти організацій з теми "Proteins Synthesis"

1

Desai, Holly E. Synthesis and Structural Characterization of Reflectin Proteins. Fort Belvoir, VA: Defense Technical Information Center, February 2012. http://dx.doi.org/10.21236/ada563720.

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2

Singh, Anjali. Amino Acids: Building Blocks of Proteins. ConductScience, June 2022. http://dx.doi.org/10.55157/cs20220612.

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Amino acids are essential organic compounds serving as protein building blocks. Recognized for their biological roles, they underpin proteins' structure and interactions. Classified by polarity and nutritional necessity, essential amino acids, not synthesized by the body, include histidine, leucine, lysine, and more, while non-essential ones are produced internally. These molecules exhibit diverse functions, from neurotransmitter precursor synthesis to immune support. Industries leverage amino acids in animal feed, artificial sweeteners, flavor enhancers, and drug manufacturing, highlighting t

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3

Barash, Itamar, and Robert E. Rhoads. Translational Mechanisms that Govern Milk Protein Levels and Composition. United States Department of Agriculture, November 2004. http://dx.doi.org/10.32747/2004.7586474.bard.

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Original objectives: The long term objective of the project is to achieve higher content of protein in the milk of ruminants by modulating the translational machinery in the mammary gland. The first specific aim of the BARD proposal was to characterize responsiveness of various experimental systems to combination of lactogenic hormones and amino acids with particular emphasis on discrimination between the control of total protein synthesis and milk protein synthesis. Based on the results, we planned to proceed by characterizing the stage of protein synthesis in which the stimulation by lactoge

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4

Barash, Itamar, and Robert Rhoads. Translational Mechanisms Governing Milk Protein Levels and Composition. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7696526.bard.

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Original objectives: The long-term goal of the research is to achieve higher protein content in the milk of ruminants by modulating the translational apparatus of the mammary gland genetically, nutritionally, or pharmacologically. The short-term objectives are to obtain a better understanding of 1) the role of amino acids (AA) as regulators of translation in bovine and mouse mammary epithelial cells and 2) the mechanism responsible for the synergistic enhancement of milk-protein mRNA polyadenylation by insulin and prolactin. Background of the topic: In many cell types and tissues, individual A

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5

Christopher, David A., and Avihai Danon. Plant Adaptation to Light Stress: Genetic Regulatory Mechanisms. United States Department of Agriculture, May 2004. http://dx.doi.org/10.32747/2004.7586534.bard.

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Original Objectives: 1. Purify and biochemically characterize RB60 orthologs in higher plant chloroplasts; 2. Clone the gene(s) encoding plant RB60 orthologs and determine their structure and expression; 3. Manipulate the expression of RB60; 4. Assay the effects of altered RB60 expression on thylakoid biogenesis and photosynthetic function in plants exposed to different light conditions. In addition, we also examined the gene structure and expression of RB60 orthologs in the non-vascular plant, Physcomitrella patens and cloned the poly(A)-binding protein orthologue (43 kDa RB47-like protein).

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6

Lapidot, Moshe, and Vitaly Citovsky. molecular mechanism for the Tomato yellow leaf curl virus resistance at the ty-5 locus. United States Department of Agriculture, January 2016. http://dx.doi.org/10.32747/2016.7604274.bard.

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Tomato yellow leaf curl virus (TYLCV) is a major pathogen of tomato that causes extensive crop loss worldwide, including the US and Israel. Genetic resistance in the host plant is considered highly effective in the defense against viral infection in the field. Thus, the best way to reduce yield losses due to TYLCV is by breeding tomatoes resistant or tolerant to the virus. To date, only six major TYLCV-resistance loci, termed Ty-1 to Ty-6, have been characterized and mapped to the tomato genome. Among tomato TYLCV-resistant lines containing these loci, we have identified a major recessive quan

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7

Grafi, Gideon, and Brian Larkins. Endoreduplication in Maize Endosperm: An Approach for Increasing Crop Productivity. United States Department of Agriculture, September 2000. http://dx.doi.org/10.32747/2000.7575285.bard.

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The focus of this research project is to investigate the role of endoreduplication in maize endosperm development and the extent to which this process contributes to high levels of starch and storage protein synthesis. Although endoreduplication has been widely observed in many cells and tissues, especially those with high levels of metabolic activity, the molecular mechanisms through which the cell cycle is altered to produce consecutive cycles of S-phase without an intervening M-phase are unknown. Our previous research has shown that changes in the expression of several cell cycle regulatory

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8

Amir, Rachel, David J. Oliver, Gad Galili, and Jacline V. Shanks. The Role of Cysteine Partitioning into Glutathione and Methionine Synthesis During Normal and Stress Conditions. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7699850.bard.

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The objective of this research is to study the nature of the competition for cysteine (Cys), the first organic sulfur-containing compound, between its two main metabolites, glutathione (GSH) and methionine (Met). GSH plays a central role in protecting plants during various stresses, while Met, an essential amino acid, regulates essential processes and metabolites in plant cells through its metabolite S-adenosyl-Met. Our results, which are based on flux analysis and measurements of Met- metabolites, show that the flux towards Met synthesis is high during non-stress conditions, however the flux

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9

Wang, X. F., and M. Schuldiner. Systems biology approaches to dissect virus-host interactions to develop crops with broad-spectrum virus resistance. Israel: United States-Israel Binational Agricultural Research and Development Fund, 2020. http://dx.doi.org/10.32747/2020.8134163.bard.

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More than 60% of plant viruses are positive-strand RNA viruses that cause billion-dollar losses annually and pose a major threat to stable agricultural production, including cucumber mosaic virus (CMV) that infects numerous vegetables and ornamental trees. A highly conserved feature among these viruses is that they form viral replication complexes (VRCs) to multiply their genomes by hijacking host proteins and remodeling host intracellular membranes. As a conserved and indispensable process, VRC assembly also represents an excellent target for the development of antiviral strategies that can b

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10

Deutsch, Christopher. Discovery and Characterization of the Proteins Involved in the Synthesis of N⁶-Threonylcarbamoyl Adenosine, a Nucleoside Modification of tRNA. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.3075.

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