Дисертації з теми "Pudica"
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1
Melkonian, Rémy. "Etude de la spécificité d’association béta-rhizobia-Mimosa : approches par l'écologie microbienne et la génomique fonctionnelle." Thesis, Montpellier 2, 2012. http://www.theses.fr/2012MON20195/document.
Анотація:
Les béta-rhizobia sont des symbiotes de légumineuses retrouvées principalement associés au genre Mimosa. Les études des symbiotes de Mimosa pudica révèlent différents profils de diversité au sein des alpha (Rhizobium spp) et béta-rhizobia (Burkholderia, Cupriavidus) le long de la ceinture tropicale, les béta-rhizobia étaient toujours majoritaires dans les nodosités de cette plante hôte. Dans ce travail de thèse, nous avons étudié cette spécificité d'association béta-rhizobia/Mimosa pudica, par une approche couplant l'étude des traits symbiotiques bactériens à l'analyse des profils d'expression de leurs génomes dans les premières étapes de la symbiose, et en comparaison avec les alpha-rhizobia. Nous avons analysé les traits symbiotiques (compétitivité pour la nodulation, efficience symbiotique) au niveau intra et interspécifique de 4 espèces de béta-rhizobia et 4 d'alpha-rhizobia. Si l'efficience symbiotique est similaire parmi toutes les souches testées, différents niveaux de compétitivité ont été trouvés selon l'espèce, B. phymatum et B. tuberum étant les plus compétitives. Les tests effectués sur différentes variétés de M. pudica montrent un effet variétal sur la compétitivité de C. taiwanensis. Les traits symbiotiques mesurés expliquent en partie les profils de diversité des symbiotes de M. pudica dans les zones d'origine (Amérique du Sud) ou en zone introduite (Taiwan). Les transcriptomes de trois bactéries ayant des traits symbiotiques différents (B. phymatum STM815, C. taiwanensis LMG19424 et R. mesoamericanum STM3625) ont été comparés (par RNAseq), pour relier les différentes réponses induites par les exsudats racinaires aux traits symbiotiques de chaque rhizobium. Chaque bactérie développe une stratégie spécifique liée à ses traits symbiotiques et à l'origine de la symbiose dans son groupe bactérienBeta-rhizobia are legume symbionts mainly found associated to the Mimosa genus. Diversity studies of Mimosa pudica symbionts in native and introduced areas reveal different diversity patterns of alpha (Rhizobium spp) and beta-rhizobia (Burkholderia, Cupriavidus), with beta-rhizobia being always the main symbionts in the nodules of this legumes species. In this thesis we have studied the symbiotic specificity between beta-rhizobia and M. pudica (and the comparison with alpha-rhizobia) by a dual approach combining the study of bacterial symbiotic traits and the analysis of their transcriptomes in the first steps of symbiosis. We analysed symbiotic traits (nodulation competitiveness, symbiotic efficiency) at intra and interspecific levels on four species of beta-rhizobia and four of alpha-rhizobia. If symbiotic efficiency is similar among all strains, different levels of competitiveness were measured with a strong strain effect largely explained by the species affiliation, B. phymatum and B. tuberum being the most competitive species. Tests on different M. pudica varieties showed an impact on the competitiveness of C. taiwanensis. Symbiotic traits explained in part the symbiont patterns observed in diversity studies in French Guiana (M. pudica native area) and Taiwan (introduced). Root-exudates induced transcriptomes of three bacteria (two beta--rhizobia: B. phymatum STM815, C. taiwanensis LMG19424 and one alpha, R. mesoamericanum STM3625) with contrasted symbiotic traits were compared (by RNAseq). Each bacterium develops a specific strategy linked to its symbiotic traits and the origin of symbiosis in its bacterial group
2
Paula, Cristiane da Silva. "Controle químico de Mimosa pudica em pastagem de Brachiaria decumbens com doses reduzidas de herbicidas /." Ilha Solteira, 2013. http://hdl.handle.net/11449/98635.
Анотація:
Orientador: Fernando Tadeu de CarvalhoBanca: Edson Lazarini
Banca: Caio Antonio Carbonari
Resumo: A ocorrência de plantas daninhas em pastagens é um dos fatores responsáveis pela queda na produtividade das plantas forrageiras e, a espécie Mimosa pudica L. (malícia) é uma das mais frequentes infestantes na região centro-oeste brasileira. O trabalho constou de dois experimentos e foi desenvolvido com o objetivo de analisar a eficácia de herbicidas hormonais recomendados para pastagens, no controle de M. pudica, considerando as doses normais e doses reduzidas dos herbicidas. O primeiro experimento foi desenvolvido no município de Inocência, MS, onde o trabalho foi realizado em campo sobre a forrageira Brachiaria decumbens Stapf, analisando-se os dados submetidos aos tratamentos sob o aspectos de eficácia. Os tratamentos utilizados foram: 2,4-D + picloran (2,0; 3,0 e 4,0 L/ha), fluroxypyr + picloran (1,0 e 2,0 L/ha), aminopiralide + 2,4-D (1,0 e 2,0 L/ha), aminopiralide + fluxipir metílico (0,5 e 1,0 L/ha), 2,4-D (2,0 L/ha) e testemunha sem herbicida. O segundo experimento foi desenvolvido em laboratório no município de Ilha Solteira, SP, onde o trabalho foi realizado em laboratório e os herbicidas foram aplicados sobre a espécie daninha M. pudica cultivada em vasos. Os tratamentos para esta fase foram os seguintes: 2,4-D + picloran (2,0; 3,0 e 4,0 L/ha), fluroxypyr + picloran (1,0 e 2,0 L/ha), aminopiralide + 2,4-D (1,0 e 2,0 L/ha) e aminopiralide + fluxipir metílico (0,5 e 2,0 L/ha). Neste experimento analisou-se também o efeito dos tratamentos sobre as plantas daninhas em relação à sua sensibilidade ao toque e em relação à taxa de ETR (taxa de caminhamento de elétrons) utilizando-se um fluorômetro. Observou-se que, no primeiro experimento, todos os tratamentos, exceto o 2,4-D isolado, controlaram eficazmente a planta daninha. No segundo experimento, os tratamentos utilizados controlaram 100% a planta daninha... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The occurrence of weeds in pastures is one of the factors responsible for the decline in productivity of forage plants and the species Mimosa pudica l. (malice) is one of the most common weeds in the central-western Brazil. The study consisted of two experiments and was developed with the aim of analyzing the efficacy of hormonal herbicides recommended for pastures to control M. pudica, considering the normal doses and low doses of herbicides. The first experiment was conducted in the municipality of Inocência, MS, where the field work was carried out on the grass Brachiaria decumbens analyzing treatments under the aspects of efficacy. The treatments for this step were as follows: 2,4-d + picloran (2.0, 3.0 and 4.0 L / ha) + fluroxypyr picloran (1.0 and 2.0 l / ha), aminopyralid + 2,4-D (1.0 and 2.0 L / ha) + aminopyralid fluxipir methyl ester (0.5 and 2.0 L/ ha), 2,4-D (2.0 L / ha) and witnesses in the bush and clean. The second experiment was conducted in single Ilha Solteira, SP, where the work was performed in the laboratory and were sprayed on the weed M. Pudica cultivated in pots. The treatments for this step were as follows: 2,4-D + picloran (2.0, 3.0 and 4.0 L / ha) + fluroxypyr picloran (1.0 and 2.0 L/ ha), aminopyralid + 2,4-D (1.0 and 2.0 L / ha) + aminopyralid and fluxipir methyl ester (0.5 and 1.0 L / ha). This experiment also examined the effect of treatments on weeds in relation to their sensitivity to touch and relative to ETR rate (rate of traversal of electrons) using a fluorometer apparatus. It was observed that in the first experiment, all treatments except 2,4-D alone, effectively controlled the weed. In the second experiment, the treatments used 100% controlled the weed and no differences in the rate of ETR and sensitivity to touch, to 3 days after application. It was also found that lower dose in all treatments that... (Complete abstract click electronic access below)
Mestre
3
REIS, Alessandra dos Santos Belo. "Lesões traumáticas na pele causadas pelos espinhos de Mimosa pudica e Mimosa debilis em equídeos." Universidade Federal do Pará, 2012. http://repositorio.ufpa.br/jspui/handle/2011/4668.
Анотація:
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CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
Foram estudadas lesões traumáticas de pele em equídeos causadas por plantas traumáticas, conhecidas popularmente como “dorme-maria”, “dormideira”, “arranhadeira”, “malícia” e “não-me-toque”. O estudo foi conduzido em uma propriedade no município de Castanhal, região Nordeste do Estado do Pará, onde foram realizadas visitas técnicas, estudo epidemiológico, coletas de sangue, biopsias de pele afetada e coleta das plantas. Foram estudados 25 equídeos, sendo 14 machos e 11 fêmeas, com idade entre seis meses e oito anos. A pastagem era constituída de Brachiaria humidicola e estava intensamente invadida pelas plantas traumatizantes. Os animais apresentaram lesões ulcerativas, de bordos irregulares, na cabeça (narinas, focinho, lábios superiores e inferiores e chanfro), na cavidade oral (vestíbulo bucal e gengiva) e nos membros (boletos, metacarpos e metatarsos e articulação escápulo-umeral). No exame histopatológico foram observados focos de erosões cutâneas, caracterizados por perda e necrose da epiderme, com espongiose e degeneração vesicular da epiderme remanescente, e leve infiltrado inflamatório na derme subjacente, constituído predominantemente por macrófagos e, em menor grau, eosinófilos. Foram identificadas duas plantas, Mimosa pudica e Mimosa debilis, ambas da família Leguminosae Mimosoideae. Baseado nos resultados obtidos pode-se concluir que as lesões de pele foram causadas pela ação traumática de Mimosa pudica e Mimosa debilis.
We studied traumatic injury of the skin in horses caused by traumatic plants, popularly known as "sleep-mary", "Poppy", "scraper", "malice" and "do not touch me". The study was conducted on a farm in the district of Castanhal, northeastern of the state of Para, where there were technical visits, epidemiological study, blood samples, biopsies of affected skin and collection of plants. The study included 25 horses, 14 males and 11 females, aged between six months and eight years. The pasture consisted of Brachiaria humidicola and was heavily invaded by traumatizing plants. The animals showed ulcerative lesions of irregular borders, on the head (nose, muzzle, upper and lower lips and chamfer), oral cavity (buccal vestibule and gum) and limbs (billets, metacarpals and metatarsals and scapular-humeral joint). The histopathological examination revealed foci of cutaneous erosions, characterized by loss and epidermal necrosis with spongiosis and vesicular degeneration of the remaining epidermis and mild inflammatory infiltrate in the underlying dermis, consisting predominantly of macrophages and, to a lesser degree, eosinophils. We identified two plants, Mimosa pudica and Mimosa debilis, both from the Leguminosae Mimosoideae family. Based on these results we can conclude that the skin lesions were caused by the traumatic action of Mimosa pudica and Mimosa debilis.
4
Paula, Cristiane da Silva [UNESP]. "Controle químico de Mimosa pudica em pastagem de Brachiaria decumbens com doses reduzidas de herbicidas." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/98635.
Анотація:
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paula_cs_me_ilha.pdf: 334753 bytes, checksum: fcd8aee2a013e0a21c4a8de75c837120 (MD5)A ocorrência de plantas daninhas em pastagens é um dos fatores responsáveis pela queda na produtividade das plantas forrageiras e, a espécie Mimosa pudica L. (malícia) é uma das mais frequentes infestantes na região centro-oeste brasileira. O trabalho constou de dois experimentos e foi desenvolvido com o objetivo de analisar a eficácia de herbicidas hormonais recomendados para pastagens, no controle de M. pudica, considerando as doses normais e doses reduzidas dos herbicidas. O primeiro experimento foi desenvolvido no município de Inocência, MS, onde o trabalho foi realizado em campo sobre a forrageira Brachiaria decumbens Stapf, analisando-se os dados submetidos aos tratamentos sob o aspectos de eficácia. Os tratamentos utilizados foram: 2,4-D + picloran (2,0; 3,0 e 4,0 L/ha), fluroxypyr + picloran (1,0 e 2,0 L/ha), aminopiralide + 2,4-D (1,0 e 2,0 L/ha), aminopiralide + fluxipir metílico (0,5 e 1,0 L/ha), 2,4-D (2,0 L/ha) e testemunha sem herbicida. O segundo experimento foi desenvolvido em laboratório no município de Ilha Solteira, SP, onde o trabalho foi realizado em laboratório e os herbicidas foram aplicados sobre a espécie daninha M. pudica cultivada em vasos. Os tratamentos para esta fase foram os seguintes: 2,4-D + picloran (2,0; 3,0 e 4,0 L/ha), fluroxypyr + picloran (1,0 e 2,0 L/ha), aminopiralide + 2,4-D (1,0 e 2,0 L/ha) e aminopiralide + fluxipir metílico (0,5 e 2,0 L/ha). Neste experimento analisou-se também o efeito dos tratamentos sobre as plantas daninhas em relação à sua sensibilidade ao toque e em relação à taxa de ETR (taxa de caminhamento de elétrons) utilizando-se um fluorômetro. Observou-se que, no primeiro experimento, todos os tratamentos, exceto o 2,4-D isolado, controlaram eficazmente a planta daninha. No segundo experimento, os tratamentos utilizados controlaram 100% a planta daninha...
The occurrence of weeds in pastures is one of the factors responsible for the decline in productivity of forage plants and the species Mimosa pudica l. (malice) is one of the most common weeds in the central-western Brazil. The study consisted of two experiments and was developed with the aim of analyzing the efficacy of hormonal herbicides recommended for pastures to control M. pudica, considering the normal doses and low doses of herbicides. The first experiment was conducted in the municipality of Inocência, MS, where the field work was carried out on the grass Brachiaria decumbens analyzing treatments under the aspects of efficacy. The treatments for this step were as follows: 2,4-d + picloran (2.0, 3.0 and 4.0 L / ha) + fluroxypyr picloran (1.0 and 2.0 l / ha), aminopyralid + 2,4-D (1.0 and 2.0 L / ha) + aminopyralid fluxipir methyl ester (0.5 and 2.0 L/ ha), 2,4-D (2.0 L / ha) and witnesses in the bush and clean. The second experiment was conducted in single Ilha Solteira, SP, where the work was performed in the laboratory and were sprayed on the weed M. Pudica cultivated in pots. The treatments for this step were as follows: 2,4-D + picloran (2.0, 3.0 and 4.0 L / ha) + fluroxypyr picloran (1.0 and 2.0 L/ ha), aminopyralid + 2,4-D (1.0 and 2.0 L / ha) + aminopyralid and fluxipir methyl ester (0.5 and 1.0 L / ha). This experiment also examined the effect of treatments on weeds in relation to their sensitivity to touch and relative to ETR rate (rate of traversal of electrons) using a fluorometer apparatus. It was observed that in the first experiment, all treatments except 2,4-D alone, effectively controlled the weed. In the second experiment, the treatments used 100% controlled the weed and no differences in the rate of ETR and sensitivity to touch, to 3 days after application. It was also found that lower dose in all treatments that... (Complete abstract click electronic access below)
5
CID, Gabriela de Carvalho. "Dermatite ulcerativa causada por espinhos de Mimosa setosa, M. debilis e M. pudica (Fam?lia Fabaceae) em equinos." Universidade Federal Rural do Rio de Janeiro, 2016. https://tede.ufrrj.br/jspui/handle/jspui/1527.
Анотація:
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CAPES
Mechanic natural skin lesions in horses caused by thorns of Mimosa spp. are described. Between the three plant species identified as responsible for the lesions, Mimosa setosa was present in greater quantity (80%) in the pasture, whilst M. debilis and M. pudica existed in lower proportion. Three ulcerative dermatitis outbreaks were observed during rainy periods of April to May 2013, December 2013 to February 2014 and April to May of the same year. Twenty-five horses from the Sector of Animal Reproduction, Federal Rural University of Rio de Janeiro, showed ulcerative skin lesions with irregular borders, hemorrhagic exudate, sometimes covered with scabs, located mainly in the regions of the pastern, fetlock, scapular-humeral joints, upper and lower lips, nose, nostrils, cheeks and chamfer. Seven horses were biopsied and histopathological examination revealed ulceration of the skin with inflammatory infiltrate by macrophages and neutrophils, delimited by granulation tissue. In some cases, microspicules of these plants (hirsute trichomes) were found throughout the inflammatory reaction. The diagnosis of skin dermatitis, caused by traumatic action of the plants, was based on the presence of Mimosa spp. in the pasture, on the characteristic clinic-pathological features and on recovery of the horses after their removal from the pasture. This appears to be the first report of the occurrence of ulcerative dermatitis caused by Mimosa setosa, as dermatitis caused by the others has been described before.
Descrevem-se les?es de natureza mec?nico-traum?tica na pele de equ?deos causadas por espinhos de Mimosa spp. Dentre as tr?s esp?cies da planta identificadas como respons?veis pelas les?es, M. setosa estava presente em maior quantidade e M. debilis e M. pudica encontravam-se em menor propor??o na pastagem. Ocorreram tr?s surtos de dermatite ulcerativa em per?odos chuvosos de abril a maio de 2013, dezembro de 2013 a fevereiro de 2014 e abril a maio deste mesmo ano. Vinte e cinco equinos do Setor de Reprodu??o Animal da Universidade Federal Rural do Rio de Janeiro apresentaram les?es ulcerativas na pele com contornos irregulares, exsudato serosanguinolento, por vezes recobertas com crostas. Localizadas principalmente nas regi?es da quartela, boleto, articula??es escapulo-umeral, l?bios superiores e inferiores, focinho, narinas, bochechas e chanfro. Sete animais foram biopsiados e o exame histopatol?gico revelou ulcera??o da epiderme e infiltrado inflamat?rio constitu?do por macr?fagos e neutr?filos, delimitado por tecido de granula??o subjacente. Em alguns casos, foram observados microesp?culos das referidas plantas (tricomas hirsutos) em meio ? rea??o inflamat?ria. O diagn?stico de dermatite cut?nea causada pela a??o traum?tica da planta baseou-se na presen?a de Mimosa spp. na pastagem, nos achados cl?nico-patol?gicos caracter?sticos e na recupera??o dos animais ap?s a retirada destes do pasto. Trata-se da primeira observa??o sobre a ocorr?ncia de dermatite ulcerativa causada por Mimosa setosa.
6
Steinmetz, Marc. "Etude pharmaco-chimique de Mimosa pudica (Mimosaceae) Elucidation structurale de constituants polyphénoliques Evaluation de leur activité anti-collagénase." Université Louis Pasteur (Strasbourg) (1971-2008), 2001. http://www.theses.fr/2001STR13205.
7
Keulder, Rozelle. "Oviposition site preference of lacewings in maize ecosystems and the effect of Bt maize on Chrysoperla pudica (Neuroptera: Chrysopidae) / Rozelle Keulder." Thesis, North-West University, 2010. http://hdl.handle.net/10394/4469.
Анотація:
Resistance development and possible non–target effects have been of
concern since the first deployment of genetically modified crops with
insecticidal properties. It is especially at the third trophic level and with
important predators such as lacewings (Chrysoperla spp.) (Neuroptera:
Chrysopidae) where negative effects of Cry 1Ab protein could have adverse
effects in agro–ecosystems. Monitoring of the effect of genetically modified Bt
maize on non–target organisms is required by law in South–Africa. Neuroptera
are excellent indicators of environmental and habitat transformation, and also
include key species for signifying areas and faunas that require priority
protection. Monitoring techniques, especially for insect eggs, are often labour
intensive and time consuming. A study was conducted to determine the
preferred oviposition site of Chrysoperla spp. on maize plants to facilitate
time–effective searching for eggs of these beneficial insects. Furthermore we
determined if the presence of aphids on plants influenced Chrysoperla spp.
oviposition preference. Another study was conducted to evaluate the effect of
indirect exposure of C. pudica to Cry 1Ab protein, through healthy Bt–maize
feeding prey, on its biology. Daily flight activity patterns and the height at
which chrysopid adults fly above the crop canopy were also determined, as
well as the movement of adult Chrysoperla spp. between maize fields and
adjacent headlands. A clear spatial oviposition pattern was observed on
maize plants and oviposition was not random as reported in earlier studies.
This data facilitates rapid monitoring of the presence of eggs in maize
cropping systems and is also of use in general pest management. Choicetest
data showed that females responded positively to host plants that were
infested with aphids. Feeding studies in which C. pudica larvae were
indirectly exposed to Bt–toxin at the 3rd trophic level, showed a limited effect
of Bt–toxin on only a few of the parameters that were evaluated. The pupal
period and percentage adult emergence of larvae exposed to an unusually
high amount of Bt–toxin was significantly shorter and lower respectively than
that of the control group. The overall result of this study, in which the possible
effect of food quality (prey) was excluded, showed that Cry 1Ab protein had an adverse affect only on certain fitness components during the life cycle of
C. pudica. However, since this study represented a worst–case scenario
where diverse prey was not available to C. pudica, negligible effects is
expected under field conditions where prey is more diverse. It was
determined that chrysopids was most active between 16:00 – 23:00 and that
they fly largely between 0.5 m – 2.5 m above ground level. An attempt was
also made to quantify migration between different vegetations types. This part
was terminated because of bad weather conditions at several occasions
when the experiment was attempted. Chrysopids were never present in
grassland vegetation, but an adjacent lucerne field maintained a large
population. As the maize crop developed chrysopid population numbers
increased inside the field, presumably originating from the lucerne field.Thesis (M.Sc (Environmental Science))--North-West University, Potchefstroom Campus, 2011.
8
Daubech, Benoît. "Évolution expérimentale d'un symbiote de légumineuse : étude des facteurs génétiques et des forces de sélection qui favorisent ou non l'évolution du mutualisme." Thesis, Toulouse 3, 2019. http://www.theses.fr/2019TOU30338.
Анотація:
La symbiose qui s'établit entre les légumineuses et les bactéries appelées rhizobia est un processus complexe qui aboutit à la formation d'un nouvel organe végétal, le nodule, dans lequel les bactéries internalisées (bactéroïdes) fixent l'azote atmosphérique au profit de leur hôte. Les rhizobia ne constituent pas un groupe taxonomique homogène. Ils appartiennent à une quinzaine de genres dispersés au sein des α- and ß-protéobactéries. Les rhizobia auraient évolué à partir du transfert horizontal de gènes essentiels à la symbiose, suivi d'une réorganisation du génome d'accueil sous pression de sélection de la plante permettant une activation et/ou optimisation du potentiel symbiotique acquis. Ce scénario évolutif a été reproduit en laboratoire par une approche d'évolution expérimentale. Le plasmide symbiotique du symbiote de Mimosa pudica, Cupriavidus taiwanensis LMG19424, a été introduit dans la bactérie pathogène de plante Ralstonia solanacearum GMI1000. A partir de cette bactérie chimère 18 lignées parallèles ont été évoluées par des cycles successifs d'inoculation à M. pudica et ré-isolation des bactéries des nodules. Après 16 cycles d'évolution, trois observations ont été faites : i) les bactéries évoluées ne fixent pas l'azote et l'évolution vers le mutualisme n'est donc pas achevée à ce stade, ii) un gène de fonction inconnue semble important pour l'infection intracellulaire, et iii) les mutations permettant l'acquisition et/ou l'amélioration de l'infection des cellules du nodule semblent également améliorer la nodulation. Afin d'identifier les conditions favorables à l'émergence du mutualisme dans l'expérience d'évolution et potentiellement dans la nature, nous avons analysé la dynamique spatio-temporelle de deux sous-populations quasi isogéniques de C. taiwanensis, l'une fixatrice d'azote (Fix+) et l'autre non fixatrice (Fix-), au cours du processus symbiotique avec M. pudica. Nous avons observé une dégénérescence précoce et sélective des Fix-, y compris lorsqu'ils partagent un même nodule avec des Fix+, et établit la cinétique d'expansion des Fix+ au cours du temps. A partir d'un modèle mathématique et de validations expérimentales, nous avons prédit que de rares Fix+ envahiraient une population majoritairement Fix- au cours de cycles successifs de nodulation avec une probabilité fonction de la taille initiale de l'inoculum, du nombre de plantes inoculées et de la longueur des cycles. Par la suite nous avons étudié le rôle d'un gène du plasmide symbiotique de C. taiwanensis, dont la délétion dans l'une des lignées était responsable d'un défaut d'infection intracellulaire. Nous avons montré que ce gène, appelé noeM, est un gène de nodulation impliqué dans la biosynthèse de facteurs Nod atypiques où le sucre réducteur est ouvert et oxydé. noeM est principalement détecté dans des isolats de plantes appartenant à la tribu des Mimoseae, et particulièrement chez les souches capables de noduler M. pudica. Les gènes noeM forment un clade phylogénétique à part et spécifique des rhizobia. Un mutant ΔnoeM de C. taiwanensis s'est avéré affecté pour la nodulation de M. pudica, confirmant son rôle dans la symbiose avec cette légumineuse. Enfin, l'analyse cytologique détaillée de l'infection racinaire de M. pudica par C. taiwanensis et quelques souches de R. solanacearum portant une mutation adaptative de l'infection intracellulaire a été initiée, afin d'analyser l'impact de ces mutations sur les étapes symbiotiques précocesThe symbiosis between legumes and bacteria, known as rhizobia, is a complex process resulting in the formation of a novel plant organ, the nodule, in which internalized bacteria (bacteroids) fix nitrogen to the benefit of the host plant. Rhizobia do not form a homogeneous taxonomic group. They belong to a dozen of genera scattered within α- and ß-proteobacteria. Rhizobia may have evolved from horizontal transfer of key symbiotic genes, followed by genome remodeling under plant selection pressure, allowing the activation and/or optimization of the acquired symbiotic potential. This evolutionary scenario is being replayed in the laboratory using an experimental evolution approach. The symbiotic plasmid of the Mimosa pudica symbiont, Cupriavidus taiwanensis LMG19424, was introduced into the plant pathogen Ralstonia solanacearum GMI1000. 18 parallel lineages were derived from this chimeric ancestor using serial cycles of inoculation with M. pudica and re-isolation of bacteria from the nodules. After 16 cycles of evolution, three observations were done: i) the evolved bacteria do not fix nitrogen and evolution towards mutualism is not completed, ii) a gene of unknown function seems to be involved in intracellular infection and iii) the mutations that allow and/or improve intracellular infection also improve nodulation capacity. To determine conditions that favor the emergence of mutualism in the laboratory and possibly in nature, we analyzed the spatio-temporal dynamics of two quasi-isogenic sub-populations of C. taiwanensis, one nitrogen-fixing (Fix+) and the other not (Fix-), along their symbiotic process with M. pudica. We observed an early degenerescence of Fix- bacteroids, even when they share a nodule with Fix+, and established the kinetics of Fix+ expansion along time. Using mathematical modeling and experimental validations, we predicted that rare Fix+ will invade a population dominated by non-fixing bacteria during serial nodulation cycles with a probability that is function of initial inoculum, plant population size and nodulation cycle length. Then, we studied the role of a C. taiwanensis symbiotic plasmid gene, whose deletion in one lineage was responsible of intracellular infection defect. We showed that this gene, called noeM, is a novel nodulation gene involved in the biosynthesis of atypical Nod factors where the reducing sugar is open and oxidized. noeM was mostly found in isolates of the Mimoseae tribe, especially in all strains able to nodulate M. pudica. The noeM genes form a separate phylogenetic clade containing only rhizobial genes. A noeM deletion mutant of C. taiwanensis was affected for the nodulation of M. pudica confirming the role of noeM in the symbiosis with this legume. Last, we initiated the detailed cytological analysis of M. pudica root infection by C. taiwanensis and a few strains bearing adaptive mutations for intracellular infection, in order to analyze the effect of these mutations on early symbiotic stages
9
MOYEN, CHRISTELLE. "Etude des modalités de l'implication et de la mobilisation du calcium lors de l'absorption de glycine par les tissus pulvinaires de Mimosa pudica L." Poitiers, 1992. http://www.theses.fr/1992POIT2337.
Анотація:
Le calcium est implique fonctionnellement dans l'absorption de glycine qui s'effectue par un cotransport proton-substrat. Dans une gamme reduite de concentrations, le calcium extracellulaire et l'ionophore a23187 stimulent le transport de glycine tandis que des chelateurs (edta et egta) le reduisent. L'intervention du calcium met en jeu des canaux calciques sensibles a differents antagonistes organiques et inorganiques et a un agoniste des canaux calciques voltage-dependants. L'absorption de glycine n'est pas affectee par le tmb-8 et le licl ce qui indique que le calcium implique est extracellulaire. La cytofluorimetrie a laser a precise qu'un apport de glycine (10 mm final) provoque une augmentation rapide et sensible a la nifedipine, de la concentration d'ions calcium cytoplasmiques libres. Les effets exerces par des antagonistes de la camoduline suggerent que cette calciproteine intervient dans l'absorption de glycine et contribue a declencher la reponse cellulaire. L'absorption de glycine est egalement diminuee par le kcl, le tetraethylammonium et le cscl ce qui suggere que les ions potassium et des canaux potassiques sont aussi mis en jeu. L'absence d'effets de la plupart de ces traitements sur la difference de potentiel membranaire des cellules motrices et sur l'excretion spontanee de protons par les tissus pulvinaires indique que les composes agissent sur le cotransport sans affecter la force proton motrice. L'analyse des lipides n'a revele aucune variation de la composition des lipides, notamment des phosphoinositides, sous l'effet de la glycine
10
Saeedi, Saed. "Observations chez Cassia fasciculata et Mimosa pudica d'effets induits sur la physiologie des pulvini par des composés phénoliques et benzoïques et étude des modalités de l'absorption de l'acide salicylique." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb37609688f.
11
Saeedi, Saed. "Observations chez cassia fasciculata et mimosa pudica d'effets induits sur la physiologie des pulvini par des composes phenoliques et benzoiques et etude des modalites de l'absorption de l'acide salicylique." Poitiers, 1987. http://www.theses.fr/1987POIT2269.
12
Correa, Vassallo Claudio. "Si pudiera lo haría peor — imagen histórica, imagen biográfica." Tesis, Universidad de Chile, 2009. http://www.repositorio.uchile.cl/handle/2250/101225.
Анотація:
El presente texto consta de dos capítulos que abordan aspectos de los géneros desde donde construyo mi trabajo: la pintura y la instalación. Este texto se fundamenta en una revisión de la pintura histórica y mi paisaje urbano directo, desde los sujetos que testimonian el relato visual, el cual queda como la imagen objetiva del suceso.
13
Wang, Guiwei. "Automatic information extraction and prediction of karst rocky desertification in Puding using remote sensing data." Thesis, Högskolan i Gävle, Samhällsbyggnad, GIS, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-23988.
Анотація:
Karst rocky desertification (KRD) is one kind of severe environmental problem existing in southwest of China. Reveal KRD condition is vital to solve the problem. A way to address the problem is by identifying KRD areas, so that policy-makers and researchers may get a better view of the issue and know where the areas affected by the problem are located. The study area is called Puding which is a county located in the central part of Guizhou province. Based on Landsat data, by using GIS and RS techniques, KRD information of Puding was extracted. Furthermore, the study monitored decades of change of the environmental problem in Puding and predicted possible condition in the future. Other researchers and decision makers may get a better view of the issue from the study results. In addition to Landsat data, other used data includes: ASTER Global digital elevation model data, Modis data, Google Earth data and other thematic maps. In the study, expert classification system and spectral features based model two methods were applied to extract KRD information and compare with each other. Their classified rules were taken from previous studies separately. Necessary preprocessing procedures such as atmospheric correction and geometrical correction were performed before extraction. After extraction relevant results were evaluated and analyzed. Predictions were made by cellular automata Markov module. Based on extracted KRD results, the distribution, percentage, change, and prediction of KRD conditions in Puding were presented. The results of the accuracy evaluation showed that the spectral features based model had acceptable performance. However, the KRD results extracted by expert classification system method were poor. The extracted KRD results, including KRD maps and the prediction map, both indicated that KRD areas in Puding were decreased from 1993 (spring) to 2016 (spring) and suggested to pay more attention to KRD areas changes with the seasons
14
Silva, Marco Felipe Martins. "Desenvolvimento de um pudim com sabor a leite condensado, novo produto da marca El Mandarin." Master's thesis, ISA/UL, 2019. http://hdl.handle.net/10400.5/18346.
Анотація:
Mestrado em Ciências Gastronómicas - Instituto Superior de Agronomia / Universidade Nova de LisboaA Mandarin – Sociedade Ibérica de Produtos Alimentares Lda. pretende produzir um pudim instantâneo com sabor a leite condensado, um novo produto da marca EL Mandarin. Como tal, o objetivo deste projeto foi obter um preparado instantâneo em pó ao qual apenas seja necessário adicionar açúcar e leite na sua confeção. Pretende-se que o produto tenha um modo de preparação semelhante ao pudim flan tradicional da marca EL Mandarin. No entanto, este novo produto deverá ter um sabor e uma textura diferenciados, semelhante à textura que se associa a um pudim de leite condensado caseiro. Selecionaram-se quatro receitas caseiras de pudim de leite condensado e efetuando uma prova de análise sensorial hedónica, com 20 provadores, aferiu-se a receita melhor cotada e com características mais interessantes, a que se considerou o produto alvo. Caracterizou-se o pudim alvo em termos de textura, através de um teste de análise de perfil de textura. Os valores de firmeza, adesividade e coesividade foram tomados como valores referência para o desenvolvimento do novo produto. Os valores nutricionais foram estimados a partir da composição dos respetivos ingredientes. A análise instrumental da cor, foi feita através de um colorímetro. Os pudins desenvolvidos foram caracterizados pelas mesmas metodologias. Realizou-se um exercício de benchmarking no mercado Nacional, que mostrou não existirem produtos homólogos em sabor, em Portugal. Verificou-se a existência de dois produtos semelhantes no Brasil. Este facto reforça o carater inovador do produto desenvolvido, tanto em termos de textura como a nível de sabor
N/A
15
Tindefjord, Norlander Anna. "Ursäkta, jag ska bara pudra näsan! : Om individers ackommodation av språkliga uttryck och grad av medvetenhet i samband med toalettbesök." Thesis, Karlstads universitet, Institutionen för språk, litteratur och interkultur (from 2013), 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-71780.
Анотація:
Den här språksociologiska undersökningen tar reda på hur individer anpassar sitt språkliga uttryck i samband med toalettbesök i privat, offentligt och högtidligt sammanhang, samt deras grad av medvetenhet och normanpassning i känsliga sammanhang. Detta har gjorts genom en enkätstudie där totalt 362 respondenter svarat på både öppna och slutna frågor, vilket har gett både kvantitativa och kvalitativa data. Det empiriska materialet har efter bearbetning jämförts med tidigare forskning och teoretiska perspektiv, vilket utmynnat i resultaten att de allra flesta ackommoderar efter sammanhang och är medvetna om hur de ackommoderar i formella sammanhang. Ytterligare en tendens resultatet visar är att det postmoderna samhället påverkat samhällsutvecklingen mot en mer öppen kommunikationsstil där relationer skapas och samarbete eftersträvas.This sociolinguistic research examines how individuals adapt their linguistic expression in relation to toilet visits in private, public and formal contexts, as well as their degree of awareness and accommodation in sensitive contexts. A total of 362 respondents, have answered a questionnaire with both open and closed questions, which has given both quantitative and qualitative data. After processing, the empirical material has been compared with former research and theoretical perspectives, concluding that a majority of the respondents accommodate according to context and are aware of how they accommodate in formal contexts. The result also tends to that the postmodern society has influenced the social progress towards a more open style of communication in which relationships are created and cooperation is sought.
16
Slovák, Marek. "Dvoudobý jednoválcový motocyklový motor s výfukovými ventily." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2012. http://www.nusl.cz/ntk/nusl-230391.
Анотація:
The master´s thesis deals with the design of two-stroke uniflow scavenged motorcycle engine according to patent František Pudil (PV 7018-80) 216305. The engine has been designed for using in off-road sport motorcycles. The objective of this thesis is to design the engine of this conception and to reveal benefits and defects of this concept by using this method. In this thesis the emphasis is put on design of the construction groups which are directly related to unconventional concept of engine. On the other hand, the parts which can be designed conventionally were solved marginally or were not solved at all. In the first part of the thesis there are thoroughly dicsussed expected benefits and disadvantages of this concept. Computational part focuses on valvetrain and porting of engine. Last part deals with design of engine parts.
17
Pudzich, Gregor Benedikt Verfasser], and Jens Martin [Akademischer Betreuer] [Gurr. "Rewriting the past, pluralizing the present : renegotiating Canadianness in the works of Dionne Brand, George Elliott Clarke and Lawrence Hill / Gregor Benedikt Pudzich ; Betreuer: Jens Martin Gurr." Duisburg, 2019. http://d-nb.info/1191694011/34.
18
Bakker, Frederik Lambertus. "The struggle of the Hindu Balinese intellectuals developments in modern Hindu thinking in independent Indonesia /." Amsterdam : VU University Press, 1993. http://catalog.hathitrust.org/api/volumes/oclc/31215765.html.
19
Chen, Sihting, and 陳思庭. "Study On Tyrosinase Inhibitor From Mimosa Pudica." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/47846927301726000134.
Анотація:
碩士國立高雄海洋科技大學
水產食品科學研究所
100
The objective of present study is to characterize the tyrosinase inhibitor from Mimosa pudica in order to provide information for its application in food or cosmetics. Tyrosinase inhibitors from Mimosa pudica were extracted by solvent or supercritical fluid. The recovery and concentration caused 50% inhibition (IC50) to tyrosinase were examined and compared. Among the efficiency of solvent extraction, that extracted with methanol showed the best efficiency, followed by those extracted with ethanol, water , hot water , ethyl acetate and hexane. The optimal extraction time was 150 mins, and IC50 was 0.053mg/g with recovery 10.13%. The optimal conditions for tyrosinase inhibitor from Mimosa pudica extracted by supercritical fluid extraction (SFE) are listed as follows: pressure, 200 atm; temperature 60 ℃; carbon dioxide flow rate, 0.8mL/min. Crude extracts of tyrosinase inhibitor from Mimosa pudica were further purified with XAD gel fractionation. An active fraction named XAD-C was obtained, the recovery and IC50 were 24.67 % and 0.023 mg/g respectively. Content of polyphenols from XAD-C was 32.28±0.06 mg/g, while flavonoids contents was 1.56±0.08 mg/g。Malvidin and Petunidin were identified in XAD-C according to LC-MS-MS analysis. Inhibition for tyrosinase inhibitor of XAD-C on enzyme activity reached the plateau when XAD-C reacted with tyrosinase for 8-10 min. This phenomenon indicated a fast-binding reaction occurred between the inhibitor XAD-C and the tyrosinase. Moreover, tyrosinase inhibitor of XAD-C did not have the ability to reduce dopaquinone,but had the ability to reduce Cu(Ⅱ) into Cu(Ⅰ). Results from enzymatic kinetics showed XAD-C belonged to a uncompetitive inhibition.
20
Tsai, Chia-fang, and 蔡佳芳. "Studies on the biological activities of Mimosa pudica." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/85996167815736323448.
Анотація:
碩士大同大學
生物工程學系(所)
100
Extracts of Mimosa pudica were cured in the treatment of headache, migraine, insomnia, diarrhea, dysentery in Taiwan and India. Some biological activities, including inhibitory melanogensis of B16F0 cell, increasing proliferation of fibroblast cells, and antioxidant activity, were determined. Results showed that viabilities of B16F0 cells treated with methanolic and hot water extracts were above 90% lie, the toxicity is low. Methanolic extract exhibited higher inhibition on melanogensis, tyrosinase activity and tyrosinase expression of B16F0 cells than those of hot water extract. In addition, these two extracts significantly increase proliferation of CCD966SK human skin fibroblast and collagen production. Extracts of Mimosa pudica were showed antioxidant activity, including radicals (DPPH, ABTS．+ and．O2-) scavenging activity, reducing power, ferrous ion-chelating capacity(FIC) and oxygen radical absorbance capacity(ORAC). The FIC of Mimosa pudica extracts was in accordance with quercetin, while ORAC was higher than those of quercetin and ascorbic acid. Methanolic extract exhibited higher antioxidant activity and amounts of total phenolics and proanthocyanidinis, showing polyphenols and proanthocyanidins of Mimosa pudica roots were contributed to their antioxidant activity. These results indicated that Mimosa pudica roots were considered to be as skin whitening and antioxidant agents.
21
"Immunomodulatory effects and toxicity of mimosa pudica, the sensitive plant." Chinese University of Hong Kong, 1993. http://library.cuhk.edu.hk/record=b5887741.
Анотація:
by Cheng Yuk Kwan, Anna.Thesis (M.Phil.)--Chinese University of Hong Kong, 1993.
Includes bibliographical references (leaves 104-112).
Acknowledgements
Table of Contents --- p.i
Abbreviations --- p.iv
Abstract --- p.vi
List of figures --- p.ix
List of tables --- p.xi
Chapter Chapter One: --- Introduction
Chapter 1.1 --- Objective and scope of the project --- p.1
Chapter 1.2 --- Literature review of Mimosa pudica
Chapter 1.2.1 --- Morphology of Mimosa pudica --- p.3
Chapter 1.2.2 --- Chemistry of Mimosa pudica --- p.5
Chapter 1.2.3 --- Uses in traditional medicine --- p.5
Chapter 1.2.4 --- Clinical and pharmacological studies of Mimosa pudica --- p.6
Chapter 1.2.5 --- Toxicology of Mimosa pudica --- p.8
Chapter 1.2.6 --- Characteristics and toxicology of mimosine --- p.9
Chapter 1.3 --- Immunomodulation
Chapter 1.3.1 --- Overview of the immune system --- p.11
Chapter 1.3.2 --- Strategies on the study of immunomodulation of Mimosa pudica --- p.13
Chapter 1.4 --- Toxicology
Chapter 1.4.1 --- Principles of the toxicological assays
Chapter 1.4.1.1 --- LD50 --- p.17
Chapter 1.4.1.2 --- Enzyme assays --- p.18
Chapter 1.4.1.3 --- Subacute toxicity test --- p.24
Chapter 1.4.1.4 --- Reproductive toxicity test --- p.25
Chapter Chapter Two: --- Materials and methods
Chapter 2.1 --- Materials
Chapter 2.1.1 --- Mimosa pudica --- p.27
Chapter 2.1.2 --- Animals --- p.27
Chapter 2.1.3 --- Chemicals --- p.28
Chapter 2.2 --- Methods
Chapter 2.2.1 --- Extraction of Mimosa pudica --- p.32
Chapter 2.2.2 --- Assays for the immunomodulatory effects of Mimosa pudica
Chapter 2.2.2.1 --- Cell preparation
Chapter a) --- Splenocytes --- p.35
Chapter b) --- Thymocytes --- p.35
Chapter c) --- Macrophages --- p.36
Chapter 2.2.2.2 --- Splenocyte proliferation --- p.37
Chapter 2.2.2.3 --- Thymocyte proliferation --- p.38
Chapter 2.2.2.4 --- Phagocytic activity of macrophages --- p.39
Chapter 2.2.2.5 --- Release of IL-1 by macrophages --- p.40
Chapter 2.2.2.6 --- Plaque forming cells --- p.41
Chapter 2.2.2.7 --- Restoration on splenocyte blastogenesis of old mice --- p.42
Chapter 2.2.3 --- Assays for the toxicity of Mimosa pudica
Chapter 2.2.3.1 --- LD50 --- p.43
Chapter 2.2.3.2 --- Enzyme assays --- p.43
Chapter 2.2.3.3 --- Subacute toxicity --- p.43
Chapter 2.2.3.4 --- Reproductive toxicity --- p.44
Chapter 2.2.4 --- Statistical analysis --- p.44
Chapter Chapter Three: --- Results
Chapter 3.1 --- Immunomodulatory effects of Mimosa pudica
Chapter 3.1.1 --- In vitro study on the lymphocyte proliferation
Chapter 3.1.1.1 --- Splenocyte proliferation --- p.45
Chapter 3.1.1.2 --- Thymocyte proliferation --- p.50
Chapter 3.1.2 --- In vivo study on the lymphocyte proliferation --- p.53
Chapter 3.1.3 --- Phagocytic activity of macrophages --- p.58
Chapter 3.1.4 --- Release of IL-1 by macrophages --- p.64
Chapter 3.1.5 --- Plaque forming cells --- p.67
Chapter 3.1.6 --- Restoration on splenocyte blastogenesis of old mice --- p.69
Chapter 3.2 --- Toxicity of Mimosa pudica
Chapter 3.2.1 --- LD50 --- p.72
Chapter 3.2.2 --- Enzyme assays --- p.75
Chapter 3.2.3 --- Subacute toxicity --- p.80
Chapter 3.2.4 --- Reproductive toxicity --- p.85
Chapter Chapter Four: --- General discussion on the immunomodulatory effects and toxicity of Mimosa pudica
Chapter 4.1 --- Immunomodulatory effects of Mimosa pudica --- p.88
Chapter 4.2 --- Toxicity of Mimosa pudica --- p.95
Chapter Chapter Five: --- Concluding remarks --- p.99
References --- p.104
Appendix --- p.113
22
KELÍŠEK, Michal. "Vliv intenzity dráždění (thigmonastie) na růst semenáčů\nl{} \kur{Mimosa pudica}." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-188489.
Анотація:
The aim of this study was to determine the effect of different frequency irritation on growth, morphology or seedling mortality of Mimosa pudica. During preparation of the experiment were seeded 200 seeds of M. pudica but only 48 plants were used due to low germination of seeds . They were divided into three groups of equal number and these groups was placed one beside the other in a warm greenhouse with the same temperature and light conditions. The experiment started when sufficient size was reached (approximately 4 leaves per plant). The first ( control ) group was not irritated, the second group was irritated 5 times per day and the third group 15 times per day. Three times during the experiment (1. 9., 11. 9. and 21. 9. 2014) were measured length and counted the number of leaves. During the last measurement was cut and weighed aboveground part of the plants. Also number of internodes in all plants was recorded. During the experiment was also monitored the number of blossoms. All measured values were entered into tables and then statistically evaluated. In the experiment was found that the different frequency irritation affects the stem length and the final weight of the plants. The effect on the number of blossoms, leaves and internodes was not shown. There was no mortality in any of three groups.
23
Hu, Chia-yu, and 胡家瑜. "PROTECTIVE EFFECTS OF MIMOSA PUDICA AGAINST UVB-INDUCED DAMAGE OF HUMAN KERATINOCYTES." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/8ue7hm.
Анотація:
碩士大同大學
生物工程學系(所)
102
Recently herbal medicines have gained an increased interest in their functional properties. Mimosa pudica, rich in polyphenolic compounds, showed high antioxidant activities and inhibitory melanogenesis. In this work, the protective effects of M. pudica methanolic extract (MPME) and M. pudica hot water extract (MPWE) against ultraviolet B (UVB) - induced damage in a human keratinocytes were evaluated. MPME and MPWE showed low cytotoxicities to HaCaT cells. With increasing UVB radiation dose, viability of HaCaT cells deceased significantly (p <0.05), increasing a high degree of damage on HaCaT cells with UVB. After irradiation with 25, 50 and 100 mJ/cm2, respectively, HaCaT cells pretreated with M. pudica showed high viability compared to those without M. pudica extracts treatments. MPME exhibited significantly better protective effect than MPWE (p <0.05). In addition, M. pudica decreased radical oxygen species generation and lipid peroxides level induced by UVB irradiation in HaCaT cells. M. pudica restored glutathione contents reduced by UVB exposure at 25mJ/cm2. These results suggested that M. pudica showed a protective effect to UVB- induced damaged in keratinocytes.
24
Chou, Chia-Jung, and 周佳蓉. "PROTECTIVE EFFECTS OF MIMOSA PUDICA AGAINST UVB-INDUCED DAMAGE OF HUMAN FIBROBLASTS." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/4ec88p.
Анотація:
碩士大同大學
生物工程學系(所)
103
Ultraviolet radiation of skin resulted in oxidative stress, causing photoaging of skin. Recently the anti-photoaging property of herbs had attracted much attention. The protective effects of Mimosa pudica against UVB-induced damage of human fibroblasts CCD966SK was determined in this study. The results showed that 100 ?慊/ml of methanol extract of M. pudica did not reduce the cell viability, indicating low cytotoxicity of M. pudica to CCD966SK cells. A dose-dependent decrease in cell viability was found in UV radiated CCD966SK cells. However, cell viability and collagen formation increased in CCD966SK cells pretreated with increasing methanol and water extracts for 24 h, respectively, followed by UVB radiation. Methanol extracts of 100 ?慊/ml exhibited the maximum effect without statistically significant difference to ascorbic acid as a positive control. This demonstrated that M. pudica increased the viability and collagen production in UVB-exposed CCD966SK cells. A significant decrease in glutathione and increase in malondialdehyde were obtained in CCD966SK cells with UVB irradiation. Pretreatment of M. pudica also promoted glutathione and reduced malondialdehyde amounts in CCD966SK cells with UVB exposure. Methanol extract had significant better effect than water extract on the CCD966SK cells exposed at 25 and 50 mJ/cm2. These results indicated that M. pudica could reduce the UVB-induced damage in human dermal fibroblasts.
25
Lai, De-Hong, and 賴德宏. "Quantitative Analysis Of Some Pyrrolizidine Alkaloids In Mimosa pudica By Gas Chromatography Mass Spectrometry." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/37363005106577461532.
Анотація:
碩士亞洲大學
保健營養生技學系碩士班
100
Mimosa pudica is a perennial herb, it can be used as Chinese herbal medicine in Taiwan. Mimosa pudica are collected from several towns in Taiwan.We proposed by gas chromatography mass spectrometry (GC-MS) to detect Mimosa pudica whether contain with liver toxicity and carcinogenicity of pyrrolizidine alkaloids. Pyrrolizidine alkaloids were isolated from the aqueous acid extracts of the above ground whole plant or roots by use of strong cation-exchange, solid-phase extraction (SCE-SPE). The sensitive analysis of PAs was identified from plant material by GC-MS method, and enhance accuracy by literature and RI values. Through the preliminary experimental result, the Mimosa pudica contain at least five pyrrolizidine alkaloids, as 9-angeloyl -trachelanthamidine、9-tigloyltrachelanthamidine、9-tigloylplatynecine、9-angeloyldihydroxyheliotridane、9-angeloylretronecine. In quantification analysis of the individual PAs was determined by linear regression curves. The methyl palmitate was examined as an internal standard examined. 9-Angeloyltrachelanthamidine figured as a major PA in Mimosa pudica (37.75 ~ 340.71μg/g). This study will provide important information to public consumers regarding the health risk of intake Mimosa pudica containing PAs.
26
Warren, Jo-Ann Francis. "The indirect effect of Cry 1Ab protein expressed in Bt maize, on the biology of Chrysoperla pudica (Neuroptera: Chrysopidae) / Jo-Ann Francis Warren." Thesis, 2014. http://hdl.handle.net/10394/10816.
Анотація:
Genetically modified (GM) maize was developed mainly to control lepidopteran pests
such as the maize stem borer (Busseola fusca) (Lepidoptera: Noctuidae). Since the
first commercialization of GM crops with insecticidal properties, possible non-target
effects such as the effect at the third trophic level on important predators for example
lacewing species (Chrysoperla spp.) have been of concern. Contradicting results
were reported in previous studies with regard to the effect of Cry 1Ab protein
produced by Bt maize on the performance of lacewings. Some studies found that Bt
proteins had no effect while others reported that C. carnea performed poorly if they
consumed prey that consumed Cry 1Ab protein. In South Africa one of the most
common chrysopid species in maize ecosystems is Chrysoperla pudica (Navás)
(Neuroptera: Chrysopidae). Evolution of Bt resistant pests, such as B. fusca in
South Africa facilitates a new pathway for exposure of predators to healthy prey that
consumes Cry 1Ab proteins. The aims of this study was to determine the effect of
the Cry 1Ab protein expressed in Bt maize on a non-target organism‟s (C. pudica)
biology via indirect exposure, and to determine the concentration of Cry 1Ab protein
in the plant, prey and predator. Chrysoperla pudica larvae were indirectly exposed
to the Bt-toxin through healthy Bt-maize feeding prey (B. fusca larvae) in two feeding
experiments and lacewing survival and life history parameters recorded. Bt had a
limited effect on some parameters that were evaluated. The larval and pupal periods
of C. pudica larvae that were exposed to the Bt-toxin had a significant difference
from that of the control treatment. The Bt-toxin had a significant effect on fecundity,
fertility and malformation after emergence of C. pudica adults of which larvae fed
only on Bt resistant B. fusca larvae, but not on the mortality rate. Cry 1Ab
concentration was the highest in the plant, followed by the prey and lacewing larvae.
This study showed that the Cry 1Ab protein had a slight adverse effect only on
certain life parameters of C. pudica, and that Cry 1Ab protein was hardly detectable
in C. pudica larvae. However, since this study represented a worst-case scenario
where diverse prey was not available, insignificant effects is expected under field
conditions where prey is diverse.MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2014
27
Lin, Shu-Ru, and 林淑茹. "The study on the relationship of acute toxicity to Pseudomonas pudita and the aromatic compounds structure." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/80231138422428807087.
Анотація:
碩士國立成功大學
化學系
87
Acute toxicity of compounds in benzene, toluene, aniline and phenol series to Pseudomonas putida (ATCC 23973) was estimated by an initial oxygen uptake method. Inhibition studies of these compounds on the oxidation of benzoate by Pseudomonas putida were expressed as oxygen uptake rates. Double reciprocal plots for the inhibition by these compounds of oxygen uptake in Pseudomonas, a physical constant, Ki, was obtained. Inasmuch as most compounds inhibit noncompetively, the relative toxicity of different compounds can be estimated by a new toxicity parameter RT (relative toxicity) which is defined as 100/Ki. In benzene and toluene series, compounds with nitro- and chloro-substituent were found more toxic than other substituted benzenes and toluenes. In phenol series, an analysis of compound toxicity in quantitative structure-activity relationship (QSAR) was also conducted. In correlation of log (1/Ki) with sustituent constant , the following two equations were obtained. log(1/Ki) = -5.73 — 1.75 = -0.17 ~ 0.00 log(1/Ki) = 3.22 + 2.63Es —1.77 = 0.00 ~ 0.55 While s > 0.55, the toxicity of compounds can not be estimated from these equations due to the fact that these compounds have low pKa (less than 8) and become ionized in the reaction condition.
28
Pudimat, Rainer [Verfasser]. "Probabilistische Modelle zur Beschreibung und Vorhersage regulativer DNA-Sequenzen / Rainer Pudimat." 2008. http://d-nb.info/995032289/34.
29
Pudzich, Robert [Verfasser]. "Synthese und Charakterisierung spiroverknüpfter Emitter- und Ladungstransportmaterialien mit kombinierten Funktionalitäten / vorgelegt von Robert Pudzich." 2004. http://d-nb.info/973640146/34.
30
Lin, Yi-hsiung, and 林義雄. "A Research into the Ecotourism Resources Survey: Take the Puding Area in Kenting as example." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/91646444639071973393.
Анотація:
碩士國立屏東教育大學
生態休閒教育教學碩士學位學程
99
Kenting National Park nurtured rich and varied ecological appearances. Puding area is relatively slow in the development of ecotourism comparing with the other areas in Kenting National Park. This study is aimed at carrying out the ecological resources survey in Puding area. Its purpose is as follows: 1.Understand the natural resources possessed by Puding area and establish its database. 2.Understand the seasonal distribution of Puding area’s natural resources. 3.Explore the planning direction of ecotourism in Puding area in order to provide the local travel agents with references for future development. Field research and document analysis method are adopted in this study. The researcher conducted ecological resources survey over a span of one year, constructed the files one by one material to form basic database and brought up the planning direction for developing ecotourism. According to the survey, a total of 125 species in 59 families of plants are recorded altogether along the routes. Also, a total of 313 species in 123 families of animals are recorded. The geological landscape mainly records the two stalactite limestone caves. The humanities historical data concern mainly about Pai-wan aborigines and numerous international affairs. On the basis of it, this study comes to the conclusion as follows: 1.The ecological resources are abundant in Puding area. 2.The distribution of ecological resources is suitable for tourism at all seasons. 3.There can be a great diversity of travel planning in Puding area. According to the findings and the speciality of its environment, some suggestions for developing ecotourism are offered. The ultimate purposes are eco-conservation and sustainable development for the local tourism industry.
31
姜伃倩. "A Comparative Study on the planned unit development-A Case Study of the Daxi Puding Camp zone expropriation area." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/jhkzv8.
Анотація:
碩士逢甲大學
都市計畫與空間資訊學系
105
Urban planning procedures are dynamic plans. They are a process of continual, dynamic feedback, and the plan’s objective is an important standard during planning. As Taiwan’s urban land development grows increasingly prosperous, the number of land-related issues continues to grow. A comprehensive survey of land development methods includes general levying, zone levying, development permits, urban renewal, urban land readjustment, and transferrable development rights. Among these, zone levying is one of the common methods for overall development, and is also a kind of “self-sufficient” developmental venture. However, because of the immense capital required for development, rash implementation can cause the government financial difficulties. Therefore, plan contents and financial balance are critical factors in determining whether or not a development plan achieves overall success. This study is an overall examination of the use of land in Taoyuan City’s “Daxi Township (Puding District) Urban Planning Zone”, and the proposed park land along its eastern strip. The proposed plan seeks to increase the rate of public transportation use through land transformation and the establishment of transfer stations, improving overall transit transfer capabilities to promote the development of neighboring districts, thereby affecting the future development of this piece of land and its neighboring plots. Relevant documents were collected analyzed to explore legal implications, and to understand the significance of financial regulations pertaining to urban planning and zone levying. SWOT analysis and cost-benefit assessment were used to explore the plan’s contents and the financial feasibility of zone levying. It is hoped that a comparison of urban planning configurations and a and a discussion of zone levying financial analysis which explores the differences between the contents of two plans and estimates the government’s development costs and post-development returns will enable selection of the best plan for the overall development of Taoyuan City’s Daxi Township Puxiang District, which will be provide to the government as a reference.
32
Freitas, Januário Jordão Azevedo de. "A Doçaria e o desenvolvimento local – Dos lugares às redes. O Património da Doçaria do médio Ave." Master's thesis, 2021. http://hdl.handle.net/10316/99464.
Анотація:
Dissertação de Mestrado em Alimentação: Fontes, Cultura e Sociedade apresentada à Faculdade de LetrasDada a atual situação económica do país, tem-se verificado uma tendência aumento de qualidade e aumento da oferta do turismo nacional. Face aos constrangimentos económicos, há uma maior dificuldade na capitalização de recursos financeiros para férias internacionais, ou um tempo de lazer familiar, vendo-se obrigados a reajustar os seus hábitos turísticos. Esse ajustamento passou por escolherem ficar dentro do próprio país e usufruir do que tão bem o caracteriza: hospitalidade, clima e gastronomia. Tendo em conta o contexto e no seguimento de uma valorização do património português, o presente trabalho de investigação irá centrar-se no património da doçaria do Médio Ave.O trabalho é desenvolvido em torno de uma investigação exploratória que teve como objetivo caracterizar o perfil do visitante enquanto consumidor de doces conventuais e saber qual o conhecimento dos mesmos, no decorrer da Feira anual da Doçaria Conventual em Guimarães. Esta Feira denominada “Doçaria no Convento” é uma mostra anual de doces conventuais, tendo decorrido no ano de 2019 entre os dias 12 e 14 de abril, no Claustro do Antigo Convento de Santa Clara - atual Câmara Municipal de Guimarães. Esta iniciativa pretende reavivar a memória de outros tempos na área dos doces, procurando manter viva a tradição da doçaria conventual, em terras vimaranenses. O local escolhido remonta a tempos passados, onde as freiras que se dedicavam às práticas religiosas, também se dedicavam à confeção de doces.Pretende-se com este trabalho, reavivar a tradição da doçaria conventual vimaranense, mas também dar a conhecer o que de melhor se faz em termos de doçaria conventual portuguesa. Uma vertente gastronómica carregada de história e costumes.
Given the current economic situation of the country, there has been an increase in quality and offer of national tourism. Considering the economic restrains, there is a higher difficulty on capitalizing economic resources for international destinations or family vacation, making people adjust their tourism habits. This readjustment led people to make a decision of staying in the country and enjoy what is so characteristic: hospitality, climate and gastronomy.Considering the context and an appreciation of Portuguese patrimony, the present research will focus on the heritage of sweets from the region of Médio Ave.A work that was developed around an exploratory investigation that had the objective of characterizing the profile of the visitor as a consumer of convent sweets and what was their knowledge, during the annual fair dedicated to conventual sweets in Guimarães. This fair called "Doçaria no Convento" is an annual show of convent sweets, which took place in 2019 between the 12th and 14th of April, in the Cloister of the Old Convent of Santa Clara - now the Municipal Council of Guimarães. This initiative aims to revive the memory of other times in the area of sweets, trying to keep alive the tradition of conventual sweets, in Vimaranian lands. The chosen place goes back to times past, where the nuns who dedicated themselves to the religious practices, also dedicated to the making of sweets.Emphasizing the intention of this work to revive the tradition of the Vincentian conventual confectionery, but also to make known what is best done in terms of Portuguese conventual sweets. A gastronomic slope loaded with history and customs.Regarding the empirical dimension of the research, it was intended to present a review of the literature on cultural heritage, from the perspective of what is material and immaterial: gastronomy and events. Starting from a general framework on the theme and area of study, focusing on the national confrere movement and with a special emphasis on territorial marketing of target areas.
33
Alves, João Afonso Hungria Magalhães Marques. "Avaliação da qualidade microbiológica, físico-química e sensorial de sobremesas lácteas prontas a consumir ao longo do tempo de armazenamento." Master's thesis, 2020. http://hdl.handle.net/10362/114091.
Анотація:
Atualmente, os consumidores procuram alimentos com melhor qualidade, pelo que essa expetativa deve manter-se desde o período de compra até ao momento do consumo. A validação do tempo de vida útil de novos produtos é muito importante, de forma a perceber se eles são seguros, se conseguem preservar as caraterísticas químicas, microbiológicas e sensoriais e se sustentam o seu valor nutricional conforme o descrito no rótulo, ao longo do tempo. O crescimento microbiano é fator-chave para se estudar o tempo de vida útil de um determinado alimento perecível. No entanto, avaliando só este parâmetro não é possível garantir a qualidade e a segurança desse produto alimentar, sendo necessário realizar, também, avaliações sensoriais e físico-químicas.
Este trabalho pretendeu caraterizar, ao nível das propriedades microbiológicas, físico-químicas e sensoriais, quatro sobremesas prontas para consumo – produtos novos da marca Condi Alimentar S.A. (Pudim Zero Flan, Pudim Zero Chocolate, Cremoso Nata-Caramelo e Cremoso Chocolate) – ao longo do seu prazo de validade, que neste caso foi de três meses (noventa dias). As sobremesas foram realizadas a nível industrial e identificadas de acordo com a fase de produção, como INÍCIO, MEIO e FIM e, de seguida, foram armazenadas a uma temperatura de 4 ± 2oC, em frigoríficos. Assim, o trabalho consistiu em: 1) apurar a qualidade microbiológica, fazendo a pesquisa/contagem de microrganismos totais a 30oC, psicrotróficos, leveduras, bolores e Enterobacteriaceae a 37oC. O grau de conformidade foi obtido por comparação com os Valores-Guia do subgrupo 1A estipulados pelo Instituto Nacional de Saúde Doutor Ricardo Jorge; 2) estudar as propriedades físico-químicas das sobremesas, através da medição de pH e da cor; 3) avaliar a aceitação dos novos produtos pelos consumidores em termos de doçura, aspeto, textura, cor e sabor, por meio de provas sensoriais hedónicas (testes de aceitação/preferência).
O trabalho desenvolvido permitiu identificar a ausência de manutenção da higiene do processo ao longo de toda a produção, com diferenças na contaminação microbiológica dos produtos recolhidos nas diferentes fases de processamento (INÍCIO, MEIO e FIM). Para além disto, algumas formulações, especialmente o Pudim Zero Chocolate, o Cremoso Nata-Caramelo e o Pudim Zero Flan (MEIO), apresentaram análises não satisfatórias ao fim de poucos dias de armazenamento. Assim, o trabalho realizado evidenciou a necessidade de se proceder a reformulações destas sobremesas e mudar alguns parâmetros no modo de processamento.
34
Sandman, Emilie. "Subluxation de la tête radiale suite au malalignement du cubitus proximal : une étude biomécanique." Thèse, 2014. http://hdl.handle.net/1866/11251.
Анотація:
Le cubitus proximal détient une courbe sagittale unique pour chaque individu, nommée « Proximal Ulna Dorsal Angulation (PUDA) ». Une reconstruction non-anatomique du cubitus proximal, suite à une fracture complexe peut engendrer une malunion, de l’arthrose et de l’instabilité. L’objectif de cette étude était d’évaluer la magnitude de malalignement au niveau de l’angulation proximale dorsale du cubitus qui causerait un malalignement radio-capitellaire, avec et sans un ligament annulaire intact. Afin d’atteindre cet objectif, une étude biomécanique fut conduite sur six spécimens frais congelés avec un simulateur de mouvement du coude. Des fractures simulées au niveau du PUDA, furent stabilisées avec une fixation interne dans cinq configurations différentes. Des images fluoroscopiques furent prises dans différentes positions du coude et de l’avant-bras, avec le ligament annulaire intact, puis relâché. Le déplacement de la tête radiale fut quantifié avec le ratio radio-capitellaire. Une interaction significative fut découverte entre les positions du coude, les angles de malalignement et l’intégrité du ligament annulaire. La subluxation de la tête radiale fut accentuée lors de la déchirure du ligament annulaire. Une augmentation de la subluxation antérieure de la tête radiale fut observée lorsque le malalignement était fixé en extension et lors de mouvements de flexion progressive du coude. D’autre part, un malalignement en flexion et une extension graduelle du coude occasionnait une subluxation postérieure. En conclusion, les résultats ont démontré l’importance d’une reconstruction anatomique du cubitus proximal, car un malalignement de 5 degrés engendre une subluxation de la tête radiale, surtout lors d’une déchirure du ligament annulaire.It has been shown that the proximal ulna has a sagittal bow, named the Proximal Ulna Dorsal Angulation (PUDA), unique for each individual. Non-anatomic reconstruction of the proximal ulna following a complex injury may lead to malunion, arthrosis and instability, hence the importance of understanding its initial anatomy. The purpose of this study was to evaluate the magnitude of angular malalignement at the proximal ulna dorsal angulation that would lead to radiocapitellar malalignement, with and without an intact annular ligament. In order to achieve our goal, a biomechanical study was conducted on six fresh frozen specimens, with an elbow movement simulator. Simulated fractures at the PUDA were stabilized with internal fixation at five different angles. Then, fluoroscopic images were taken in different elbow and forearm positions, first with the annular ligament intact and then released. Radial head displacement was quantified with the Radio-Capitellar-Ratio (RCR). Overall, a significant interaction was found between elbow positions, angles of malalignement and annular ligament integrity. Radial head subluxation was emphasized when the annular ligament was ruptured. Moreover, anterior subluxation of the radial head increased as malalignement was fixed into extension and with progressive elbow flexion. Furthermore, posterior subluxation increased with malalignement into flexion and with elbow extension. In conclusion, our results demonstrate the importance of obtaining an anatomic reconstruction, specific for each individual’s unique proximal ulna dorsal angulation, following a proximal ulna fracture. Indeed, malalignment of 5 degrees can lead to abnormal tracking of the radial head, especially when associated with annular ligament tear.