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Статті в журналах з теми "Rapid on-site evaluation staining":

1

Thierry, Rausch, Rochat Marie-Christine, Moret Marièle, Braunschweig Richard, and Mihaescu Anca. "Modified technique of toluidine blue staining in rapid on-site evaluation." Diagnostic Cytopathology 40, no. 9 (June 16, 2011): 847–48. http://dx.doi.org/10.1002/dc.21753.

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Hewer, Ekkehard, and Anja M. Schmitt. "Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears." Acta Cytologica 64, no. 4 (2020): 375–77. http://dx.doi.org/10.1159/000505254.

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Rapid on-site evaluation (ROSE) is one of cytopathology’s “unique selling propositions.” The quality, speed, and ease of handling of the staining used is a critical factor for the efficacy of the ROSE procedure. Here, we describe a modification of rapid toluidine blue staining that can be performed within 25 s, provides excellent nuclear morphology, and is compatible with subsequent Papanicolaou staining of the slides. Furthermore, exposure to hazardous chemicals is minimized, as no organic solvents other than the alcohol-based fixative and glycerin for temporary mounting and coverslipping are required. We have used this protocol successfully in our ROSE practice and have not observed any discrepancies between toluidine blue- and permanent Papanicolaou-stained slides.
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Porfyridis, Ilias, Georgios Georgiadis, Michalis Michael, Frangiskos Frangopoulos, Paris Vogazianos, Alexis Papadopoulos, Panayiota Kara, Charis Charalampous, and Andreas Georgiou. "Rapid on-site evaluation with the Hemacolor rapid staining method of medical thoracoscopy biopsy specimens for the management of pleural disease." Respirology 21, no. 6 (April 15, 2016): 1106–12. http://dx.doi.org/10.1111/resp.12799.

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Louw, Mercia, Karen Brundyn, Pawel T. Schubert, Colleen A. Wright, Chris T. Bolliger, and Andreas H. Diacon. "Comparison of the quality of smears in transbronchial fine-needle aspirates using two staining methods for rapid on-site evaluation." Diagnostic Cytopathology 40, no. 9 (February 14, 2011): 777–81. http://dx.doi.org/10.1002/dc.21628.

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Soans, Suchetha, Lorenzo M. Galindo, and Fernando U. Garcia. "Mucin Stain on Frozen Sections." Archives of Pathology & Laboratory Medicine 123, no. 5 (May 1, 1999): 378–80. http://dx.doi.org/10.5858/1999-123-0378-msofs.

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Abstract Context.—The importance of frozen-section diagnoses in the practice of pathology cannot be overemphasized. In some cases, the use of a mucin stain can greatly aid in the diagnosis. Since few methods for mucin staining have been described that can be used in the frozen-section setting, we developed one such staining procedure for mucin. Objective.—To develop a rapid mucicarmine staining technique to be used on frozen sections that does not significantly delay overall turnaround time. Design.—A standard mucicarmine staining technique was modified by using a concentrated mucicarmine stain and a microwave oven, to reduce the total staining time to 3 minutes or less. Frozen tissue from normal colonic mucosa was used as a control, and skin from extramammary Paget disease for evaluation of margins was used as a case. Results.—The rapid mucicarmine stain successfully demonstrated the presence of mucin on frozen-tissue sections. Mucin stained deep rose, and the connective tissue stained green. Conclusion.—This rapid and simple mucin staining technique can be used on frozen sections with no significant effect on the overall turnaround time, thereby aiding rapid diagnosis on frozen sections.
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Shiratori, Yasutoshi, Takashi Ikeya, and Kenji Nakamura. "Comparison of Papanicolaou and Diff‐Quick staining in pancreatic endoscopic ultrasound‐fine needle aspiration biopsy with rapid on‐site cytologic evaluation." Digestive Endoscopy 32, no. 3 (March 2020): 434. http://dx.doi.org/10.1111/den.13604.

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Agarwal, Priyanjali, Pampa Ch Toi, Hema Subramaniam, and S. Apoorva Lakshmi. "Prospective comparison of cytological specimen adequacy assessment by different rapid staining techniques for rapid on‐site evaluation in fine needle aspiration cytology and their cost‐effectiveness." Diagnostic Cytopathology 47, no. 5 (December 25, 2018): 469–74. http://dx.doi.org/10.1002/dc.24139.

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Shiratori, Yasutoshi, Kenji Nakamura, Kazuki Yamamoto, Takeshi Okamoto, Takashi Ikeya, and Katsuyuki Fukuda. "Sa1445 COMPARISON OF PAPANICOLAOU AND DIFF-QUICK STAINING IN PANCREATIC ENDOSCOPIC ULTRASOUND-FINE NEEDLE ASPIRATION BIOPSY WITH RAPID ON-SITE CYTOLOGIC EVALUATION." Gastrointestinal Endoscopy 91, no. 6 (June 2020): AB194. http://dx.doi.org/10.1016/j.gie.2020.03.1241.

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Chambers, Michael, Konrad Krall, and Shantel Hébert-Magee. "Falling under the umbrella cells: A single institutional experience and literature review of urothelial carcinoma presenting as a primary pancreatic mass on endoscopic ultrasound-guided fine-needle aspiration." CytoJournal 14 (March 20, 2017): 6. http://dx.doi.org/10.4103/1742-6413.202601.

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Metastases to the pancreas are much less common than primary pancreatic lesions, and there are few reports in the literature of metastatic urothelial carcinoma (UC) found in the pancreas. We report two cases of metastatic UC mimicking a primary pancreatic lesion. Two female patients, aged 48 and 83 years, presented with isolated pancreatic lesions causing obstructive jaundice suspicious for pancreatic adenocarcinoma and underwent endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) with rapid on-site evaluation (ROSE). On cytopathology, the lesions were found to be UC, confirmed with immunohistochemical (IHC) staining. UC rarely metastasizes to the pancreas, and diagnosis through EUS-FNA can be challenging. However, the utilization of ROSE, dedicated cell block passes, and IHC have proved to be effective in obtaining this unusual pancreatic diagnosis by EUS-FNA.
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Stanley, R., R. Appleyard, M. McGee, S. Callary, K. Nilsson, and J. R. Field. "An evaluation of prosthetic femoral head impact on acetabular articular cartilage in a hemiarthroplasty model." Veterinary and Comparative Orthopaedics and Traumatology 22, no. 02 (2009): 142–47. http://dx.doi.org/10.3415/vcot-08-02-0020.

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Summary Objectives: The quantitation of the impact of hemiarthroplasty currently involves clinical outcome measures and radiographic evaluation. This study used in vivo and in vitro means in order to evaluate articular cartilage changes following hemiarthroplasty in the sheep. Methods: Radiostereometric analysis (RSA) was utilized in order to quantitate migration (penetration) in vivo of the femoral head into the articular cartilage of the acetabulum. Dynamic stiffness of retrieved specimens was quantitated in vitro using a handheld device. Chondrocyte viability was assessed using live/dead cell staining and quantitated using image analysis techniques. Saffranin-O staining provided histological assessment of the cellular response to hemiarthroplasty. Results: RSA showed rapid penetration of the femoral head into the acetabular cartilage over six months. Significantly higher levels of dynamic stiffness were observed in the acetabula following hemiarthroplasty. Confocal imagery highlighted changes in chondrocyte distribution and morphology. A loss of metachromasia, fibrovascular tissue invasion and apoptosis were observed histologically. Conclusions: RSA and measurement of the dynamic mechanical characteristics of cartilage provide a means of evaluating the development of degenerative articular cartilage changes in response to hemiarthroplasty.

Дисертації з теми "Rapid on-site evaluation staining":

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Björnsson, Hanna. "Comparison and optimization of May-Grunwald Giemsa and May-Grunwald Giemsa Quick Stain for morphological assessment of pleural and ascites effusions." Thesis, Uppsala universitet, Institutionen för medicinsk cellbiologi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-445869.

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Introduction: Effusion cytology can be performed for the purpose of diagnosis, treatment, and prognosis of malignant disease. A common analysis of effusion cytology samples is the May Grunwald Giemsa stain.    Aim: The aim of the study was to compare May Grunwald Giemsa stain and May Grunwald Giemsa Quick Stain in order to determine the best quality stain and suggest ways to improve the current staining protocol.     Materials and Methods: The methods used in this study are the routine laboratory’s standard procedures for  May-Grunwald Giemsa stain and May-Grunwald Giemsa Quick Stain but with adapted washing steps that investigates the effect of tap water, distilled water, and phosphate buffer on stain quality. Two pleural effusion samples were stained in the initial experiment and two pleural effusions and one ascites sample in the second experiment.    Results and Conclusion: All samples gave a greater score when stained with May-Grunwald Giemsa Quick Stain compared to traditional May-Grunwald Giemsa stain. For the traditional May-Grunwald Giemsa, the use of any of the three phosphate buffers scores higher than the routine washing where tap water is used. In conclusion, it would be of benefit to further investigate and implement phosphate buffer in traditional staining or proceed with the May-Grunwald Quick Stain for all pleural and ascites effusions.
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"VIDEO RATE STRUCTURED ILLUMINATION MICROSCOPY FOR RAPID ON-SITE PATHOLOGY EVALUATION." Tulane University, 2017.

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acase@tulane.edu
New technology for ex vivo microscopy is needed to deliver point of care pathology, which can benefit margin assessment and biopsy screening for cancer management. Current permanent histopathology method requires fixation, sectioning and staining. This process is very labor intensive and time consuming which prevents pathology analysis at point of care. We believe that structured illumination microscopy (SIM) of rapidly stained ex vivo tissues can be used to address the needs in these areas. SIM is a wide-field optical sectioning technique that uses patterned illumination to preferentially modulate and retain the in-focus object information separately from the out-of-focus background. Its distinct advantage is that SIM is a wide-field technique, which is light efficient, and the speed is decoupled from the field of view. We will develop and optimize a video rate structured illumination microscope (VR-SIM) to potentially used for point of care pathology for cancer management. Cancer resection surgery remains the primary intervention for most solid tumors. The complete surgical resection of the tumors associated with cure in nearly half of all patients. Incomplete surgical resection, as determined by the presence of residual tumor cells at the surface of the excised specimen, is the primary prognostic indicator for local tumor recurrence and decreased overall survival in a number of organ sites, where positive surgical margin (PSM) rates can exceed 50% for the most advanced tumors. Providing microscopic images of the entire tissue surface to the pathologist during the operation would enable detection of the presence and location of PSMs in time to correct the surgery and significantly reduce the need for harmful and costly salvage treatments. Unfortunately, the technical barrier to progress has been that no fluorescence microscopy method yet described for this application was fast enough to cover large tissue surface areas in 20 minutes, with the resolution to resolve nuclear atypia. When a patient presented with cancer related symptoms or a tumor, the next step in the work up for diagnosis is core needle biopsy, where typically 4-14 cores are obtained in a single biopsy session. However, the current false negative rate of the initial biopsy is up to 30% due to the lack of a fast and accurate point-of-care pathology tool. Diagnostic screening of biopsy tissue is also a concern in biospecimen banking for research purposes. The objective of the biobanking technician is to selectively obtain and preserve as much diseased tissue as possible. However, current limitations in rapid histopathology result in missed opportunities due to random sampling with no immediate feedback and wasted tissue due to destructive techniques for diagnostic confirmation. The design criteria for a point of care pathology technology is: • Be able to cover large tissue area with histological sensitivity within clinical relevant time frame o Margins assessment: image large tissue surfaces ( 60 cm2) of the margin to identify PSMs o Diagnostics Biopsy: image 8-14 biopsies of varying needle size • Easy to use and non-destructive The goal of this work is to optimize VR-SIM for accurate, high-throughput, non-destructive diagnostic imaging of fluorescently stained cancer biospecimens in point-of-care timeframes. In this work, we developed a VR-SIM system to meet the needs of imaging thick tissue and validate the system with tissue-mimicking phantoms. We compared the performance of VR-SIM with confocal microscope in thick tissues, and investigated the use of different strategies to improve VR-SIM for large specimen imaging. We determined the optimal imaging parameters to achieve the optimum performance metrics (speed, resolution, and contrast) for point-of-care prostate biopsy and surgical margin imaging, and we conducted pilot clinical studies to evaluate the feasibility of VR-SIM imaging for in-procedure surgical and biopsy guidance.
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Mei Wang

Книги з теми "Rapid on-site evaluation staining":

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Cai, Guoping, and Adebowale J. Adeniran, eds. Rapid On-site Evaluation (ROSE). Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-21799-0.

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Feng, Jing, Wen Ning, Dianhua Jiang, Jingyu Chen, and Bo Wu, eds. Rapid On-Site Evaluation (ROSE) in Diagnostic Interventional Pulmonology. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-0939-1.

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Feng, Jing, Qiang Li, Hongwu Wang, Faguang Jin, and Hongmei Zhou, eds. Rapid On-Site Evaluation (ROSE) in Diagnostic Interventional Pulmonology. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-3910-7.

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Feng, Jing, Xin Li, Pei Li, Qiang Li, and Yi Shi, eds. Rapid On-Site Evaluation (ROSE) in Diagnostic Interventional Pulmonology. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-3456-6.

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Feng, Jing, Bo Wu, Erlie Jiang, Wen Li, and Chen Meng, eds. Rapid On-Site Evaluation (ROSE) in Diagnostic Interventional Pulmonology. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-5246-5.

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Adeniran, Adebowale J., and Guoping Cai. Rapid On-site Evaluation: A Practical Guide. Springer, 2019.

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7

Zhou, Hongmei, Qiang Li, Jing Feng, Hongwu Wang, and Faguang Jin. Rapid On-Site Evaluation in Diagnostic Interventional Pulmonology : Volume 3: Neoplastic Diseases. Springer, 2020.

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8

Li, Xin, Qiang Li, Jing Feng, Yi Shi, and Pei Li. Rapid On-Site Evaluation in Diagnostic Interventional Pulmonology : Volume 1: Infectious Diseases. Springer, 2019.

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9

Feng, Jing, Wen Ning, and Dianhua Jiang. Rapid On-Site Evaluation in Diagnostic Interventional Pulmonology : Volume 2: Interstitial Lung Diseases. Springer, 2020.

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Evaluation, development, and verification of field methods for rapid, on-site determination of appropriate chemical protective clothing: Project summary. Cincinnati, OH: U.S. Environmental Protection Agency, Risk Reduction Engineering Laboratory, 1989.

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Частини книг з теми "Rapid on-site evaluation staining":

1

Tsuchida, Takaaki, and Takehiro Izumo. "Rapid On-Site Cytology Evaluation." In Respiratory Endoscopy, 67–73. Singapore: Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-287-916-5_11.

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Bongiovanni, Massimo. "Rapid on Site Evaluation (ROSE)." In Encyclopedia of Pathology, 1–2. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-319-28845-1_5072-1.

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Cai, Guoping. "Cytological Evaluation During Intraoperative Consultation." In Rapid On-site Evaluation (ROSE), 387–405. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-21799-0_14.

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Cai, Guoping, and Adebowale J. Adeniran. "Overview." In Rapid On-site Evaluation (ROSE), 3–11. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-21799-0_1.

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Cai, Guoping. "Lung." In Rapid On-site Evaluation (ROSE), 267–304. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-21799-0_10.

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Abi-Raad, Rita, and Guoping Cai. "Mediastinum." In Rapid On-site Evaluation (ROSE), 305–23. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-21799-0_11.

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Cai, Guoping. "Pancreas." In Rapid On-site Evaluation (ROSE), 327–63. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-21799-0_12.

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Abi-Raad, Rita, and Guoping Cai. "Gastrointestinal Tract." In Rapid On-site Evaluation (ROSE), 365–84. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-21799-0_13.

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Levi, Angelique W., and Guoping Cai. "Economics, Regulations, and Trends in Practice." In Rapid On-site Evaluation (ROSE), 407–12. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-21799-0_15.

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Adeniran, Adebowale J. "Telecytopathology." In Rapid On-site Evaluation (ROSE), 413–20. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-21799-0_16.

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Тези доповідей конференцій з теми "Rapid on-site evaluation staining":

1

Shah, Umang C., Arpan C. Shah, Chandrakant M. Shah, Udayan Kachchhi, Hetal V. Shah, and Palak U. Shah. "How Rapid Can “Rapid On-Site Evaluation (ROSE)” Be - Our Experience with Toluidine Blue Staining - Concordance Between Rose and Final Cytological Diagnosis on Ebus TBNA Samples." In ERS International Congress 2020 abstracts. European Respiratory Society, 2020. http://dx.doi.org/10.1183/13993003.congress-2020.2852.

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Zarogoulidis, Pavlos, Konstantinos Porpodis, Dimitris Xatzibougias, Theodora Tsiouda, and Daniil Daniilidis. "EBUS elastography for rapid on site lymph node evaluation; A useful tool?" In ERS International Congress 2019 abstracts. European Respiratory Society, 2019. http://dx.doi.org/10.1183/13993003.congress-2019.pa3071.

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3

Shetty, Prashanth, Rahul Mogal, Andrew Barlow, and Anthony Maddox. "Endobronchial/ Endoscopic Ultrasound Examination and Rapid On-Site Evaluation- Experience In A UK Centre." In ERS International Congress 2019 abstracts. European Respiratory Society, 2019. http://dx.doi.org/10.1183/13993003.congress-2019.pa4779.

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Jin, Weizhong, and Limin Wang. "Role of rapid on site evaluation in preliminary diagnosis of pulmonary infection during bronchoscopy." In ERS International Congress 2019 abstracts. European Respiratory Society, 2019. http://dx.doi.org/10.1183/13993003.congress-2019.pa4788.

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Jin, Weizhong, and Limin Wang. "Role of rapid on-site evaluation in electromagnetic navigation bronchoscopy-guided peripheral lung biopsy." In ERS International Congress 2017 abstracts. European Respiratory Society, 2017. http://dx.doi.org/10.1183/1393003.congress-2017.pa3773.

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Yarmus, LB, MP Napier, DM Dressel, and TE Van der Kloot. "A Randomized Prospective Trial of Rapid On-Site Evaluation of Transbronchial Needle Aspirate Specimens." In American Thoracic Society 2009 International Conference, May 15-20, 2009 • San Diego, California. American Thoracic Society, 2009. http://dx.doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a5769.

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Khan, A., M. Ali, C. Nixon, S. Bhele, J. Mueller, and E. K. Dinino. ""The Empty Rose": Rapid On-Site Evaluation Can Mislead When Clinical Suspicion Is High." In American Thoracic Society 2021 International Conference, May 14-19, 2021 - San Diego, CA. American Thoracic Society, 2021. http://dx.doi.org/10.1164/ajrccm-conference.2021.203.1_meetingabstracts.a2319.

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Abundo, Michael Lochinvar S., Mario Buhali, Ma Rosario Concepcion O. Ang, and Arjay Cayetano. "Development of an integrated multi-site & multi-device rapid evaluation tool for tidal energy planning." In 2012 11th International Conference on Environment and Electrical Engineering (EEEIC). IEEE, 2012. http://dx.doi.org/10.1109/eeeic.2012.6221449.

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Perez Morales, Maria, María Morales González, Cayo Javier García Polo, Enrique José González-Moya Rodriguez De Mondelo, and Mercedes Merino Sánchez. "Concordance between pulmonologist performed rapid on-site evaluation (ROSE) and pathological diagnosis in EBUS-TBNA samples." In ERS International Congress 2020 abstracts. European Respiratory Society, 2020. http://dx.doi.org/10.1183/13993003.congress-2020.5122.

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Navani, N., G. Kocjan, A. Capitanio, E. Sage, and SM Janes. "The Utility of Rapid On-Site Evaluation of Samples from Endobronchial Ultrasound Guided Transbronchial Needle Aspiration." In American Thoracic Society 2009 International Conference, May 15-20, 2009 • San Diego, California. American Thoracic Society, 2009. http://dx.doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a5788.

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