Добірка наукової літератури з теми "Scat DNA"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся зі списками актуальних статей, книг, дисертацій, тез та інших наукових джерел на тему "Scat DNA".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Статті в журналах з теми "Scat DNA"
1
Pearson, S. K., S. S. Tobe, D. A. Fusco, C. M. Bull, and M. G. Gardner. "Piles of scats for piles of DNA: deriving DNA of lizards from their faeces." Australian Journal of Zoology 62, no. 6 (2014): 507. http://dx.doi.org/10.1071/zo14059.
Повний текст джерелаАнотація:
Non-invasive genetic sampling using scats has a well established role in conservation biology, but has rarely been applied to reptiles. Using scats from captive and wild Egernia stokesii (Squamata, Scincidae) we evaluated two storage and six DNA-extraction methods and the reliability of subsequent genotype and sequence data. Accurate genotype and sequence data were obtained from frozen and dried captive lizard scat DNA extracted using a QIAamp® DNA Stool Mini Kit and a modified Gentra® Puregene® method, but success rates were reduced for wild lizard scats. Wild E. stokesii eat more plants than their captive counterparts, possibly resulting in scat DNA extracts containing plant compounds that inhibit PCR-amplifications. Notably, reliable genotypes and sequences were obtained from wild E. stokesii scat DNA extracted using a Qiagen DNeasy® Plant Mini Kit, a method designed to remove plant inhibitory compounds. Results highlight the opportunity for using scat-derived DNA in lizard studies, particularly for species that deposit scats in piles.
2
Ruibal, Monica, Rod Peakall, Andrew Claridge, and Karen Firestone. "Field-based evaluation of scat DNA methods to estimate population abundance of the spotted-tailed quoll (Dasyurus maculatus), a rare Australian marsupial." Wildlife Research 36, no. 8 (2009): 721. http://dx.doi.org/10.1071/wr09086.
Повний текст джерелаАнотація:
Context. DNA extracted non-invasively from remotely collected scat samples has been used successfully to enumerate populations of a few endangered mammal species. However, scat DNA surveys relying on scent-marking behaviours need to identify if age- or sex-specific variations or seasonal changes in scat scent-marking patterns affect population estimates. Furthermore, owing to the low quantity and quality of scat DNA, a thorough assessment of the technique is needed when it is applied to different species to ensure that individual identification is reliable. Aims. In the current study, microsatellite genetic profiles derived from 208 remotely collected scats of the spotted-tailed quoll (Dasyurus maculatus), a rare Australian marsupial carnivore, were compared with DNA profiles from tissue of 22 live-trapped individuals from the same study area to critically assess the reliability of the non-invasive method to estimate population abundance. Methods. Scat samples were collected at scent-marking sites over 4 consecutive months (April–July 2005), 7 weeks of which overlapped with the trapping program to allow direct comparisons of population estimates. Key results. Combining a multiple-tubes approach with error checking analyses provided reliable genetic tags and resulted in the detection of the majority of the live-trapped population (18 of 22 individuals). Ten additional individuals not known from trapping were also observed from scat DNA. A longer-term sampling regime was required for scats than for trapping to allow direct detection of a large proportion of the population and to provide a comparable population estimate. Critically, the 4-month scat collection period highlighted the importance of performing scat surveys during the mating season when scat scent marking is more frequent, and to avoid sex and age biases in scat marking patterns. Implications. Non-invasive scat DNA sampling methods that rely on scent-marking behaviours need to consider the duration of the sampling period and temporal differences in behaviours by the sexes and age groups to ensure that meaningful population estimates are achieved.
3
Marks, Clive A., Frank Gigliotti, Steve McPhee, Maxine P. Piggott, Andrea Taylor, and Al S. Glen. "DNA genotypes reveal red fox (Vulpes vulpes) abundance, response to lethal control and limitations of contemporary survey techniques." Wildlife Research 36, no. 8 (2009): 647. http://dx.doi.org/10.1071/wr08109.
Повний текст джерелаАнотація:
Context. Scat genotyping has not been routinely used to measure fox (Vulpes vulpes) abundance and our study sought to provide a benchmark for further technique development and assessment of field methods. Aims. This study sought to provide a comparative assessment of some common methods used to determine fox density and contrast their success with scat DNA genotyping. Methods. DNA recovered from fox scats was used to genotype individual red foxes and determine their abundance at four transects. Population indices were also developed from bait take, scat counts and sand plot tracks using index-manipulation-index (IMI) procedures on the same transects. Known samples of foxes were taken from two treatment transects using cyanide delivered in the M-44 ejector to manipulate the population and to recover foxes at the end of the trial. Key results. Replicated counts on a 41-km-spotlight transect at the field site before and after the population manipulation had low variance and good correlation (r2 = 0.79, P < 0.01). Scat genotypes revealed 54 foxes in eight days and, when combined with biopsy DNA from recovered foxes, a minimum known to be alive (KTBA) density of between 1.6 and 5 foxes km–1 was calculated for the transects. Overall, 15/30 (50%) of all recovered foxes had not been detected by scat genotyping, 23/53 (49%) of KTBA genotypes were detected only once and 5/54 (9.5%) of foxes were found to have moved between two transects. Conclusions. At transects where population manipulation occurred, surviving individuals contributed significantly more scats than at the control transects and some individuals were detected at bait stations at a much greater frequency. This strongly suggested that they had contributed disproportionately to some IMI density estimates that were probably influenced by a change in the activity of some individuals rather than changes in population density alone. At one transect, eight foxes were confirmed to be present by spotlight surveys and were detected by scat and KTBA genotypes, yet were undetected by scat, bait station and sand plot indices. Implications. Scat and other DNA-based survey techniques provide a great deal of information about the identification and movement of individuals and if DNA sampling methods can be made more efficient they have the potential to provide accurate abundance estimates that are independent of the control technique.
4
Davies, Chris, Wendy Wright, Faye Wedrowicz, and Fiona E. Hogan. "A DNA toolbox for non-invasive genetic studies of sambar deer (Rusa unicolor)." Australian Mammalogy 42, no. 1 (2020): 58. http://dx.doi.org/10.1071/am18032.
Повний текст джерелаАнотація:
Invasive sambar deer (Rusa unicolor) are having significant detrimental impacts on natural environments in south-eastern Australia. Little, however, is known about their ecology, limiting evidence-based management strategies directed at reducing deer impacts. Genetic data, generated from DNA isolated from deer scats, can be used to fill ecological knowledge gaps. This study outlines a non-invasive genetic sampling strategy by which good-quality DNA from a single deer scat can be used to determine (1) species of origin, (2) sex and (3) a unique DNA profile. DNA from deer tissue and sambar deer scat samples were used to develop and optimise molecular methods to collect reliable genetic information. A DNA toolbox is presented that describes how to find, collect and store scat samples, isolate DNA and use molecular markers to generate informative genetic data. Generating genetic data using this approach will support studies aimed at acquiring ecological knowledge about sambar deer. Such knowledge will be critical for developing evidence-based recommendations to improve on-ground management decisions for sambar deer.
5
Pikuta, Elena V., Takashi Itoh, Paul Krader, Jane Tang, William B. Whitman, and Richard B. Hoover. "Anaerovirgula multivorans gen. nov., sp. nov., a novel spore-forming, alkaliphilic anaerobe isolated from Owens Lake, California, USA." International Journal of Systematic and Evolutionary Microbiology 56, no. 11 (November 1, 2006): 2623–29. http://dx.doi.org/10.1099/ijs.0.64198-0.
Повний текст джерелаАнотація:
A novel, alkaliphilic, obligately anaerobic bacterium, strain SCAT, was isolated from mud sediments of a soda lake in California, USA. The rod-shaped cells were motile, Gram-positive, formed spores and were 0.4–0.5×2.5–5.0 μm in size. Growth occurred within the pH range 6.7–10.0 and was optimal at pH 8.5. The temperature range for growth was 10–45 °C, with optimal growth at 35 °C. NaCl was required for growth. Growth occurred at 0.5–9.0 % (w/v) NaCl and was optimal at 1–2 % (w/v). The novel isolate was a catalase-negative chemo-organoheterotroph that fermented sugars, proteolysis products, some organic and amino acids, glycerol, d-cellobiose and cellulose. It was also capable of growth by the Stickland reaction. Strain SCAT was sensitive to tetracycline, chloramphenicol, rifampicin and gentamicin, but it was resistant to ampicillin and kanamycin. The G+C content of the genomic DNA was 34.2 mol%. Major fatty acid components were C14 : 0, iso-C15 : 0, C16 : 1 ω9c and C16 : 0. 16S rRNA gene sequence analysis of strain SCAT showed a similarity of approximately 97 % with the type strains of Clostridium formicaceticum and Clostridium aceticum in clostridial cluster XI and a similarity of less than 94.2 % to any other recognized Clostridium species and those of related genera in this cluster. Strain SCAT was clearly differentiated from C. formicaceticum and C. aceticum based on comparison of their phenotypic properties and fatty acid profiles, as well as low levels of DNA–DNA relatedness between strain SCAT and the type strains of these two species. Therefore, strain SCAT is considered to represent a novel species of a new genus, Anaerovirgula multivorans gen. nov., sp. nov., in clostridial cluster XI. The type strain is SCAT (=ATCC BAA-1084T=JCM 12857T=DSM 17722T=CIP 107910T).
6
Alam, MS, MA Rahaman, RA Begum, and RM Shahjahan. "Non-invasive DNA extraction for molecular identification of royal Bengal tiger Panthera tigris tigris." Dhaka University Journal of Biological Sciences 30, no. 2 (July 9, 2021): 325–30. http://dx.doi.org/10.3329/dujbs.v30i2.54657.
Повний текст джерелаАнотація:
The flagship animal species of Sundarbans, the Royal Bengal tiger (Panthera tigris tigris) is under threat of extinction. Its natural population is declining day by day. So, to avoid killing and harming the animal, the use of non-invasive samples such as scat, hair, or scent is preferred for DNA extraction and subsequent genotyping of tiger species. DNA has been extracted from scat samples of the Bengal tiger in the present study, and a fragment of the cytochrome b gene has been sequenced after PCR with species-specific primers. DNA has been extracted manually using a previously described methodology with slight modifications. The size of the PCR product and sequence of cytochrome b gene indicates that tiger DNA is successfully extracted from scat samples using tigerspecific primers. Thus, presence of tiger DNA can be detected by using this method just by the PCR product size in the gel. This is the first report of a partial sequence of mitochondrial cytochrome b gene of P. t. tigris from Bangladesh.
Dhaka Univ. J. Biol. Sci. 30(2): 325-330, 2021 (July)
7
Grueber, Catherine E., Rowena Chong, Rebecca M. Gooley, Elspeth A. McLennan, Vanessa R. Barrs, Katherine Belov, and Carolyn J. Hogg. "Genetic analysis of scat samples to inform conservation of the Tasmanian devil." Australian Zoologist 40, no. 3 (January 2020): 492–504. http://dx.doi.org/10.7882/az.2020.005.
Повний текст джерелаАнотація:
Recent advances in molecular genetics have enabled a great deal of information about species to be obtained from analysis of non-invasively collected samples such as scat. Scat provides genetic information via residual host DNA on the outside of the scat, via characterising the genetic makeup of intestinal microbes that are present in the scat, or by examining the DNA remnants of prey items that have passed through the animal’s digestive tract. In this review, we provide a case study to demonstrate how these approaches are being used to better understand the threatened Tasmanian devil in the landscape, and to support the species’ conservation. Scat analysis enables us to quantify the genetic diversity of remote populations, where trapping is logistically challenging. We are beginning to learn how conservation management impacts the microbiome of threatened species, and investigate how various management strategies may be impacting the diverse array of bacteria and viruses that devils, like all animal species, are host to. We are using scat samples to better understand the interaction between devils and other animals in their environment by learning more about what they eat. We explore the strengths and challenges of these approaches by comparing our work to that conducted in other species. Finally, we provide specific examples of how our results are being integrated into conservation strategy for the devil.
8
Wang, Ya-Wen, Ji-Lei Zhang, Jian-Gang Jiao, Xiao-Xia Du, Samwel Mchele Limbu, Fang Qiao, Mei-Ling Zhang, Dong-Liang Li, and Zhen-Yu Du. "Physiological and metabolic differences between visceral and subcutaneous adipose tissues in Nile tilapia (Oreochromis niloticus)." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 313, no. 5 (November 1, 2017): R608—R619. http://dx.doi.org/10.1152/ajpregu.00071.2017.
Повний текст джерелаАнотація:
Visceral adipose tissue (VAT) and subcutaneous adipose tissue (SCAT) have different structures and metabolic functions and play different roles in the regulation of the mammal endocrine system. However, little is known about morphology and physiological and metabolic functions between VAT and SCAT in fish. We compared the morphological, physiological, and biochemical characteristics of VAT and SCAT in Nile tilapia and measured their functions in energy intake flux, lipolytic ability, and gene expression patterns. SCAT contained more large adipocytes and nonadipocytes than VAT in Nile tilapia. VAT had higher lipid content and was the primary site for lipid deposition. Conversely, SCAT had higher hormone-induced lipolytic activity. Furthermore, SCAT had a higher percentage of monounsaturated and lower polyunsaturated fatty acids than VAT. SCAT had higher mitochondrial DNA, gene expression for fatty acid β-oxidation, adipogenesis, and brown adipose tissue characteristics, but it also had a lower gene expression for inflammation and adipocyte differentiation than VAT. SCAT and VAT have different morphological structures, as well as physiological and metabolic functions in fish. VAT is the preferable lipid deposition tissue, whereas SCAT exhibits higher lipid catabolic activity than VAT. The physiological functions of SCAT in fish are commonly overlooked. The present study indicates that SCAT has specific metabolic characteristics that differ from VAT. The differences between VAT and SCAT should be considered in future metabolism studies using fish as models, either in biomedical or aquaculture studies.
9
McInnes, Julie C., Rachael Alderman, Bruce E. Deagle, Mary‐Anne Lea, Ben Raymond, and Simon N. Jarman. "Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates." Methods in Ecology and Evolution 8, no. 2 (November 7, 2016): 192–202. http://dx.doi.org/10.1111/2041-210x.12677.
Повний текст джерела
10
Vynne, C., M. R. Baker, Z. K. Breuer, and S. K. Wasser. "Factors influencing degradation of DNA and hormones in maned wolf scat." Animal Conservation 15, no. 2 (November 15, 2011): 184–94. http://dx.doi.org/10.1111/j.1469-1795.2011.00503.x.
Повний текст джерелаДисертації з теми "Scat DNA"
1
Stover, Rachyl-anne. "Description of the dietary breadth and overlap of the translocated Shark Bay rufous hare-wallaby (Lagorchestes hirsutus) and banded hare-wallaby (Lagostrophus fasciatus) using scat DNA." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2025. https://ro.ecu.edu.au/theses/2922.
Повний текст джерелаАнотація:
Conservation translocations are increasingly used worldwide to prevent extinctions and support ecological restoration projects. These translocations involve a lot of uncertainty, particularly when species are introduced to ecosystems where they have not previously coexisted. Many historical translocations in Australia and globally have failed due to insufficient baseline data and inadequate post-translocation monitoring. Contemporary translocations can aim to improve translocation outcomes by increasing baseline data collection and developing robust post-release monitoring for translocated species. The development of environmental DNA (eDNA) techniques has facilitated the collection of information from environments with minimal disturbance to species. The advancement of such passive monitoring techniques has allowed for increased ability to monitor and study cryptic and rare species.
The Shark Bay rufous hare-wallaby (Lagorchestes hirsutus bernieri) and the banded hare-wallaby (Lagostrophus fasciatus) were introduced to Dirk Hartog Island (DHI) from founder populations on the Bernier and Dorre islands in Shark Bay, Western Australia, as part of the Dirk Hartog Island National Park Ecological Restoration Program. Once found across large areas of south-west and central Australia, the populations on the Bernier and Dorre islands are the last natural populations of the two species due to habitat loss and predation post-European colonisation. As the rufous hare-wallaby and banded hare-wallaby have not been previously recorded on DHI, there was uncertainty regarding their successful establishment on the island and their potential interactions with each other and other species involved in the restoration program.
The diets of rufous hare-wallabies and banded hare-wallabies were investigated through the DNA metabarcoding of scat samples from three islands in Shark Bay, Western Australia. This researched aimed to assess dietary overlap and the potential for resource competition between the two hare-wallaby species. Additionally, the difference in diet between the founder populations to their post-translocation diets on DHI were analysed as an indication of dietary flexibility and adaptability to environmental changes. The diets of both species of hare-wallaby were found to be broad, containing taxa from multiple plant families including invasive weeds. On DHI there was a high degree of overlap in the diets of the two species, indicating a risk of resource competition. The diets of the translocated populations differed significantly from the founder populations, which demonstrated flexible foraging behaviour and signified that rufous hare-wallabies and banded hare-wallabies are excellent candidates for future translocation projects on other islands and within mainland reserves.
This thesis is the first study to define and compare the diets of the Shark Bay rufous hare-wallaby and the banded hare-wallaby using scat DNA. The understanding of diet is fundamental to ecology and an essential consideration in restoration projects involving new species interactions. The findings from this research can benefit the fulfilment of translocation success criteria related to species establishment and health targets for the new populations. By demonstrating the dietary breadth and flexibility of Shark Bay’s hare-wallabies, this thesis exemplifies the utility of scat DNA in supporting the conservation of translocated fauna.
2
Alamri, Sarah. "COMPARATIVE ANALYSIS OF SOYBEAN (GLYCINE MAX) ACCESSIONS USING INTER SIMPLE SEQUENCE REPEAT (ISSR) AND RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS." Thesis, Laurentian University of Sudbury, 2014. https://zone.biblio.laurentian.ca/dspace/handle/10219/2201.
Повний текст джерелаАнотація:
Soybean (Glycine max) is an important crop in the world in terms of total production and usage. It is also among the least diverse species. The main objectives of the present study were 1) to determine differences between ISSR and RAPD marker systems in detecting genetic variation in soybeans and 2) to identify and characterize accession- diagnostic molecular markers in G. max accessions. Genomic DNAs from 108 G. max accessions from 11 different gene pools were analyzed using several ISSR and RAPD primers. The levels of polymorphic loci detected with the two marker systems were in general moderate and similar.. Overall, 82% of genetic distance values were above 0.40 based on ISSR analysis. However, RAPD data revealed that the accessions from different countries are closely related with 64% genetic distance values below 0.40. The dendrograms constructed with ISSR data revealed that the South Korean accessions formed an out-group while the RAPD analysis showed that accessions from Sweden were separate from the other 10 gene pools. One variety-diagnostic marker generated with ISSR 5 primer was identified in the accession Kao Chien Tao from China. This marker was cloned, and sequenced. Although RAPD and ISSR marker systems detected similar levels of genetic variability, they target different regions of the soybean genome, resulting in different clustering of the 11 gene pools indicating different genetic relatedness among them. This finding demonstrates the usefulness of both marker systems in assessing diversity and relatedness among Glycine max gene pools.
3
Wang, Ying-Hui. "Molecular interaction of zinc finger domain : study of androgen receptor DNA binding domain and SCA7 domain of Ataxin7 by NMR." Strasbourg, 2010. http://www.theses.fr/2010STRA6018.
Повний текст джерелаАнотація:
La voie de signalisation du récepteur des androgènes (AR) est impliquée dans la progression du cancer de la prostate, et il a été montré que des mutations dans ce domaine étaient responsables de l'activation constitutive des gènes placés sous le contrôle des hormones androgènes. Une de ces mutations transforme un résidu thréonine du DBD en alanine (T575A). Des expériences permettant de mesurer l'activité de transcription ont permis à l'équipe du Dr. Ceraline à l'IRCAD de montrer que la mutation T575A induit un changement de spécificité du récepteur. Alors que l'activité de promoteurs placés sous le contrôle d'éléments de réponse spécifique de AR diminue, celle des promoteurs placés sous le contrôle d'éléments non spécifique augmente. Ce changement de spécificité est corrélé à une modification de l'affinité du récepteur pour les éléments de réponse spécifiques et non spécifiques. Afin de comprendre le mécanisme de cette "reprogrammation" à l'échelle moléculaire, l'étude structurale des domaines DBD des récepteurs sauvage et muté a été entreprise par RMN. La comparaison des deux structures en solution a montré que la mutation n'altère pas le repliement du domaine et donc que la différence de reconnaissance des éléments de réponse n'est pas liée directement à la structure tridimensionnelle du domaine. Nous avons ensuite cherché à déterminer si l'altération de la fonction n'était pas due à une différence de dynamique de la chaîne peptidique. Afin d'étudier les mouvements moléculaires le long de la chaîne, des mesures de relaxation hétéronucléaire ont été effectuées et ont montré également une grande similarité dans le comportement dynamique des deux domaines, à l'exception d'une région située dans le premier doigt de zinc à proximité d'une histidine (H570), qui est conservée dans l'ensemble de la famille des domaines DBD des récepteurs nucléaires. Cette différence nous a conduit à mesurer, par RMN, le pKa de cette histidine pour les deux protéines. Nous avons ainsi montré que la mutation T575A induit une diminution de 0,5 unité de pH par rapport à la même histidine dans le domaine sauvage. L'analyse de la structure a permis de montrer que cette différence de pKa est liée à la perte d'une interaction entre le groupe hydroxyle de la thréonine 575 et le cycle imidazole de l'histidine. L'effet de la mutation sur le mécanisme de reconnaissance s'explique donc par un effet indirect dans lequel un acide aminé situé à distance de la région d'interaction modifie la surface électrostatique du domaine DBD. L'effet de la charge positive en position 570 sur la spécificité de reconnaissance de l'élément de réponse a ensuite été étudiée en construisant plusieurs mutants portant ou non une charge à cette position (mutants H570R et H570A). Ces études ont permis de confirmer l'importance de cette charge et l'ensemble de nos travaux fournissent un éclairage inédit sur les mécanismes de reconnaissance de l'ADN par les récepteurs nucléaires. .The androgen receptor (AR) is a ligand-activated transcriptional factor and a member of the nuclear receptor super family. AR shares a common structural and functional architecture with other members of nuclear receptors. The DNA binding domain of AR (ARDBD) binds to specific response elements as a homodimer. In the clinic, certain mutations in AR are associated with the progression of prostate cancer and have consequences for the treatment of patients with advanced prostate cancer. Previous studies showed that the mutation T575A, locating in the DNA binding domain, enhances the transcriptional activity regulated by full-length AR on promoters containing the non-specific response element compared to the wild type domain does not. These differences prompted us to study the molecular mechanism of ARDBD wild type and the T575A mutant. Structures of ARDBD wild type and T575A mutant revealed high similarity. However, dynamic behavior showed distinct differences between wild type and T575A mutant domains. The protonation state of H570 in ARDBD was found to be differed by the mutation. This loss of charge of H570 results in changes in transcriptional activity of AR. .
4
Queiroz, Vagner Tebaldi de. "Obtenção de primers microssatélite e desenvolvimento, validação e mapeamento de marcadores SCAR em feijoeiro-comum." Universidade Federal de Viçosa, 2004. http://www.locus.ufv.br/handle/123456789/8784.
Повний текст джерелаАнотація:
Submitted by Marco Antônio de Ramos Chagas (mchagas@ufv.br) on 2016-10-05T19:00:00Z
No. of bitstreams: 1
texto completo.pdf: 1608244 bytes, checksum: 73ced9bf50274d34a09508edd0498859 (MD5)Made available in DSpace on 2016-10-05T19:00:01Z (GMT). No. of bitstreams: 1 texto completo.pdf: 1608244 bytes, checksum: 73ced9bf50274d34a09508edd0498859 (MD5) Previous issue date: 2004-09-20
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Embora apresente várias limitações, a técnica de RAPD ainda representa a principal ferramenta utilizada no Programa de Melhoramento Genético do Feijoeiro- comum BIOAGRO/UFV. Para contornar os problemas apresentados por esta técnica, a estratégia utilizada por vários pesquisadores tem sido a conversão dos marcadores RAPD em marcadores SCAR e o desenvolvimento de primers microssatélite. Assim, no presente trabalho foram desenvolvidos 5 primers SCAR para mancha-angular, 5 primers para ferrugem e 7 primers para antracnose. Os primers desenvolvidos para mancha-angular derivaram-se dos marcadores OPM02 425 , OPBA16 669 , OPH13 490 e OPH14+AA19 400 e foram mapeados, em acoplamento, a 5,3, 7,1, 5,6 e 10,1 cM, dos respectivos genes de resistência. Os primers desenvolvidos para ferrugem originaram-se dos marcadores OPAE19 890 , OPX11 550 , OPAJ18+AH10 700 , mas apenas o primer sAE19 foi validado. Este foi mapeado, em repulsão, a 1,0 cM do gene de resistência Ur-11. Os primers para antracnose foram desenvolvidos, a partir da seqüência dos fragmentos OPAZ20 940 , OPY20 830 , OPC08 900 , OPZ04 560 , OPB03 1800 , OPZ09 950 , OPH18 830 . Destes, apenas os primers sAZ20, sY20, sC08, sZ04 foram mapeados, em acoplamento, a 7,1, 1,2, 7,8 e 2,9 cM, dos respectivos genes de resistência. Os primers microssatélite foram desenvolvidos, a partir das seqüências de fragmentos de DNA genômico de 3 pequenas bibliotecas enriquecidas. As bibliotecas enriquecidas para as repetições GGC, CCA e AT permitiram a seleção de 79, 172 e 50 clones positivos, respectivamente. Do total de 301 clones que foram identificados, 153 já foram seqüenciados. Observou-se que 40 (26%) clones seqüenciados apresentaram fragmentos de DNA contendo microssatélite, os quais variaram quanto ao tipo, número e tamanho. As seqüências apresentaram repetições de di-, tri-, tetra- e até pentanucleotídeos. Em um mesmo fragmento, foram encontrados até 3 microssatélites. Entre as seqüências analisadas, foram observadas repetições perfeitas, imperfeitas e compostas, variarando entre 12 e 24 pb. Até o momento, foram desenhados e testados 10 pares de primers, sendo um deles originado da seqüência do marcador RAPD OPAZ20 940 . Destes, apenas 5 pares (FCctc001, FCggc001, FCccg001, FCccg002 e FCgca001) amplificaram fragmentos com tamanho esperado e bem definidos. Entretanto, esses primers foram monofórficos, quando testados entre diferentes cultivares andinos e mesoamericanos. Os primers SCAR, desenvolvidos e validados no presente trabalho, foram testados juntamente com 45 pares de primers microssatélite comerciais, entre os genitores de uma população de 154 RIL ́s. Dos primers selecionados, 7 foram mapeados em 10 grupos de um mapa parcial de ligação já existente e 1 permaneceu não-ligado. O mapa de ligação sofreu pequena variação no tamanho (247,8/252 cM) e no número de grupos de ligação (9/10 grupos). Entretanto, pela análise de variância foram constatadas associações significativas desses marcadores com diferentes características quantitativas. As associações mais significativas foram constatadas por meio de análises de regressão stepwise, as quais promoveram alteração no valor de R 2 da regressão múltipla para algumas das características. As características MAT (número de dias até a maturação), VAPLA (número médio de vagens por planta), SEPLA (número médio de sementes por planta) e PRVAG (produção média por vagem), que apresentavam valores de R 2 de 40,14; 28,99; 14,03 e 17,13 , tiveram um aumento para 44,30; 34,53; 21,92 e 26,03, respectivamente. A inclusão do marcador sH13 no GL 07 possibilitou a identificação de um novo QTL para a característica VAPLA. Este marcador também mostrou-se associado ao QTL, anteriormente, descrito para SEPLA. O marcador BM165 foi mapeado no GL 02 e mostrou-se associado a 4 QTLs diferentes, identificados para as características MAT, P100 (peso de 100 sementes), SEPLA e PRVAG.
The RAPD technique represents the major molecular tool for assisting the common bean breeding program of the BIOAGRO/UFV, in spite of its limitations. The conversion of the RAPD markers into SCAR ones, as well as the development of SSR primers are the strategy adopted by several researchers in order to relieve these problems. Therefore, 5 primers SCAR to angular leaf spot, 5 primers to rust and 7 primers to anthracnose were developed. The primers developed to angular leaf spot were derived from the RAPD markers OPM02 425 , OPBA16 669 , OPH13 490 and OPH14+AA19 400 and were mapped, in coupling phase, at 5.3, 7.1, 5.6 and 10.1 cM, from their respective resistance genes. The primers developed to rust were originated from the RAPD markers OPAE19 890 , OPX11 550 , OPAJ18+AH10 700 , although only the primer sAE19 was validated. It was mapped, in repulsion, at 1.0 cM distance from the resistance gene Ur-11. The primers developed for anthracnose were obtained from the fragment sequences of OPAZ20 940 , OPY20 830 , OPC08 900 , OPZ04 560 , OPB03 1800 , OPZ09 950 , OPH18 830 , from wich, only the primers sAZ20, sY20, sC08, sZ04 were validated. They were mapped, in coupling phase, at 7.1, 1.2, 7.8 and 2.9 cM, from their respective resistance genes. The SSR primers were developed from the sequences of the genomic DNA fragments obtained from three small enriched libraries. The libraries were enriched for the repetitions GGC, CCA and AT and made possible the selection of 79, 172 e 50 positive clones, respectively. From the total of 301 positive and identified clones, 153 were sequenced. From these clones, 40 (26%) presented DNA fragments containing microsatellite that showed variations for type, number, and size. The sequences showed repetitions of two, three, four, and five nucleotides. In the same DNA fragment, a total up to three microsatellites were found. Among the analyzed sequences, some perfect, imperfect, and compound repetitions ranging from 12 to 24 bp were found. Until this moment, only 10 pairs of primers were designed and tested; one of them was originated from the RAPD marker OPAZ20 940 . From those, only 5 pairs (FCctc001, FCggc001, FCccg001, FCccg002 e FCgca001) amplified the well-defined fragments with the expected size. However, those primers were monomorphics ones, when tested among different andean and mesoamerican cultivars. The developed and validated SCAR primers were tested together with 45 pairs of commercial SSR primers between the genitors of 154 RIL populations. From the selected primers, 7 were mapped into 10 groups of a partial linkage map already available in the lab, whereas one stayed discoupled. The linkage map was slightly altered in its size (247.8/252 cM) and number of linkage groups (9/10 groups). Variance analysis detected siginificative associations of these markers with different quantitative traits. The most significative associations were detected by stepwise regression analysis that promoted alteration in R 2 values of the multiple regression analysis for some traits. The R 2 values of the traits MAT (the number of days until the bean maturation), VAPLA (the average pod number per plant), SEPLA (the average seed number per plant) and PRVAG (the average yield per pod) increased from 40.14, 28.99, 14.03 and 17.13 to 44.30, 34.53, 21.92 and 26.03, respectively. The marker sH13 included into LG 07 allowed for the identification of a new QTL for the trait VAPLA. This marker also showed to be associated with the QTL previously described for SEPLA. The marker BM165 was mapped in LG 02 and showed to be associated with four different QTLs identified for the traits MAT, P100 (100-seed weight), SEPLA and PRVAG.
Tese importada do Alexandria
5
Oliete, Calvo Paula. "Study of the SAGA deubiquitination module: identification of new modulators and its implication on Spinocerebellar Ataxia Type 7." Doctoral thesis, Universitat Politècnica de València, 2017. http://hdl.handle.net/10251/86155.
Повний текст джерелаАнотація:
Regulation of chromatin by epigenetic modifications is a fundamental step during the control of gene expression in eukaryotic cells. The participation of different factors including histone chaperones, chromatin remodeling complexes and histone-modifying complexes regulate chromatin dynamics and ensure the correct metabolism of transcripts that need to be exported to the cytoplasm. In these lines, post-translational modifications including monoubiquitination of histone H2B (H2Bub1) and methylation of histone H3 represent a well-studied histone cross-talk which is required for chromatin integrity and transcription. Additionally, the transition from H2Bub1 to its deubiquitinated form by Ubp8, the DUB enzyme from SAGA (Spt-Ada-Gcn5-acetyltranferase) co-activator complex, is fundamental to obtain a correct gene expression. In this work, we demonstrate that the histone chaperone Asf1 and the Ran-binding protein Mog1, participate in maintaining correct levels of H2Bub1. We show that Mog1 is required for the trimethylation of histone H3 at lysine 4 (H3K4me3), hence, acting as a modulator of histone cross-talk. Mog1 role into gene expression is also demonstrated by its physical and genetically interaction with transcription factors including SAGA and COMPASS complexes. Indeed, we demonstrate that Mog1 interacts genetically with TREX-2 subunits and affects mRNA export. During this work, we have also focused in understanding the molecular mechanisms surrounding Spinocerebellar Ataxia Type 7 (SCA7) which is a rare disease caused by amino acid glutamine (Q) repeats within the DUBm component, ATXN7. Therefore, our interest has been directed towards the study of new mechanisms that trigger SCA7 such as the DUB activity from SAGA complex, protein-protein interaction networks and metabolic profiles.La regulación de la cromatina a través de modificaciones epigenéticas es un paso fundamental durante el control de la expresión génica en células eucariotas. La participación de diferentes factores tales como chaperonas de histonas, complejos de remodelación de la cromatina y complejos modificadores de histonas, regulan la dinámica de la cromatina y garantizan el correcto metabolismo de los transcritos que necesitan ser exportados al citoplasma. De esta forma, las modificaciones postraduccionales que incluyen la monoubicuitinación de la histona H2B (H2Bub1) y la metilación de la histona H3 representan un "cross-talk" de histonas la cual es requerida para la integridad de la cromatina y la transcripción. Además, la transición de H2Bub1 a su forma desubicuitinada por Ubp8, la enzima DUB del complejo co-activador SAGA (Spt-Ada-Gcn5-acetiltranferasa), es necesaria para obtener una expresión génica correcta. En este trabajo, se demuestra que la chaperona de histona Asf1 y la proteína de unión a Ran, Mog1, participan en el mantenimiento de los niveles de H2Bub1. Se demuestra que Mog1 es necesaria para la trimetilación de la histona H3 en la lisina 4 (H3K4me3), actuando como un modulador del "cross-talk" de histonas. El papel de Mog1 en la expresión génica también se demuestra por sus interacciones físicas y genéticas con factores de transcripción, incluyendo los complejos SAGA y COMPASS. Además, demostramos que Mog1 interactúa genéticamente con subunidades de TREX-2 y afecta a la exportación de mRNAs. Durante este trabajo, también nos hemos centrado en la comprensión de los mecanismos moleculares que envuelven a la Ataxia Espinocerebelosa Tipo 7 (SCA7), que es una enfermedad rara causada por una repetición de aminoácidos glutamina (Q) dentro del componente del DUBm, ATXN7. Por lo tanto, nuestro interés se ha dirigido hacia el estudio de nuevos mecanismos que desencadenan SCA7, como la actividad DUB del complejo SAGA, las interacciones proteína-proteína y los perfiles metabólicos.
La regulació de la cromatina a través de modificacions epigenètiques és un pas fonamental durant el control de l'expressió gènica en cèl·lules eucariotes. La participació de diferents factors tals com chaperones d'histones, complexos de remodelació de la cromatina i complexos modificadors d'histones, regulen la dinàmica de la cromatina i garanteixen el correcte metabolisme dels transcrits que necessiten ser exportats al citoplasma. D'aquesta forma, les modificacions postraduccionals que inclouen la monoubicuitinació de la histona H2B (H2Bub1) i la metilació de la histona H3 representen un "cross-talk" d'histones la qual és requerida per a la integritat de la cromatina i la transcripció. A més, la transició d'H2Bub1 a la seua forma desubicuitinada per Ubp8, l'enzim DUB del complex co-activador SAGA (Spt-Ada-Gcn5-acetiltranferasa), és necessària per a obtenir una expressió gènica correcta. En aquest treball, es demostra que la chaperona de histona Asf1 i la proteïna d'unió a Ran, Mog1, participen en el manteniment dels nivells d'H2Bub1. Es demostra que Mog1 és necessària per a la trimetilació de la histona H3 en la lisina 4 (H3K4me3), actuant com un modulador del "cross-talk" d'histones. El paper de Mog1 en l'expressió gènica també es demostra per les seues interaccions físiques i genètiques amb factors de transcripció, incloent els complexos SAGA i COMPASS. A més, vam demostrar que Mog1 interactua genèticament amb subunitats de TREX-2 i afecta a l'exportació de mRNA. Durant aquest treball, també ens hem centrat en la comprensió dels mecanismes moleculars que envolten a l'Atàxia Espinocerebelosa Tipus 7 (SCA7), que és una malaltia rara causada per una repetició d'aminoàcids glutamina (Q) dins del component del DUBm, ATXN7. Per tant, el nostre interès s'ha dirigit cap a l'estudi de nous mecanismes que desencadenen SCA7, com l'activitat DUB del complex SAGA, les interacciones proteïna-proteïna i els perfils metabòlics.
Oliete Calvo, P. (2017). Study of the SAGA deubiquitination module: identification of new modulators and its implication on Spinocerebellar Ataxia Type 7 [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/86155
TESIS
6
Silva, Luiz Henrique da. "O fenômeno de lente térmica em amostras de DNA livre circulante de pacientes com malignidade e sãos, investigado por meio da técnica de varredura-Z." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/43/43134/tde-13042017-014509/.
Повний текст джерелаАнотація:
No presente estudo investigou-se amostras de plasma com DNA livre circulante (DNA LC) por meio da técnica Varredura Z. Esta é uma técnica eficiente na determinação de parâmetros de diferentes materiais, tais como cristais líquidos, ferrofluidos e compostos biológicos. Esta experiência é realizada através da focalização de um feixe laser de perfil gaussiano numa amostra. Na medida em que a amostra se aproxima do foco da lente, a intensidade do feixe aumenta e alcança seu valor máximo no ponto focal, então diminui para pontos distantes do foco. Na região próxima ao ponto focal se amplificam os fenômenos não-lineares. Recentemente foi demonstrado que níveis elevados de DNA LC no plasma ocorrem com frequência em pacientes com vários tipos de câncer, podendo ser utilizados para discriminar pacientes com malignidade de pessoas saudáveis. As amostras de DNA LC, submetidas ao experimento Varredura Z, forneceram respostas ópticas devido ao fenômeno de lente térmica. Os resultados revelaram que a amplitude de lente térmica das amostras extraídas do plasma de pacientes com malignidade difere daquela de doadores sãos. A técnica Varredura Z se mostrou mais vantajosa em relação a outras biológicas porque revelou uma maior diferença entre os grupos estudados e tem o caráter de detectar mudanças estruturais no DNA LC.In the present study plasma samples with cell-free DNA were investigated by means of the Z-Scan technique. This is a powerfull technique in determining parameters of different materials, such as liquid crystals, ferrofluids and biological compounds. This experiment is performed by focusing a Gaussian profile laser beam on a sample. As the sample approaches the focus of the lens, the intensity of the beam increases and reaches its maximum value at the focal point, then decreases to points distant from the focus. In the region near the focal point non-linear phenomena are amplified. It has recently been demonstrated that high levels of plasma cell-free DNA occur frequently in patients with various cancers and can be used to discriminate patients with malignancy from healthy donors. The cell-free DNA samples, submitted to the Z-Scan experiment, provided optical responses due to the thermal lens phenomenon. The results revealed that the thermal lens amplitude of samples extracted from the plasma of patients with malignancy differs from that of healthy donors. The Z-Scan technique was more advantageous than other biological ones because it revealed a greater difference between the studied groups and has the character of detecting structural changes in cell-free DNA.
7
Shrestha, Ujjwal. "Automatic Liver and Tumor Segmentation from CT Scan Images using Gabor Feature and Machine Learning Algorithms." University of Toledo / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1522411364001198.
Повний текст джерела
8
Mokhele, Tshediso Andrew. "The application of DNA fingerprinting and marker-assisted backcross selection in breeding for sunflower high oleic acid content lines / by Tshediso Andrew Mokhele." Thesis, North-West University, 2013. http://hdl.handle.net/10394/9793.
Повний текст джерелаАнотація:
Sunflower (Helianthus annuus L.) high oleic acid content lines differ from conventional sunflower by an increase in oleic acid (C18:1) content of more than 60%. The current sunflower cultivars under production in South Africa are standard sunflower with high levels of linoleic acid (C18:2). The aim of this study was to improve the quality of oil produced by local sunflower germplasm with respect to oleic acid through employing a marker-assisted breeding technique to facilitate and speed up the recovery of the high oleic acid allele into the background of the recurrent parent genome. Eleven sunflower breeding genotypes with high and low oleic acid traits were obtained from the Agricultural Research Council-Grain Crops Institute (ARC-GCI) in Potchefstroom. The breeding genotypes were phenotypically characterised based on their oleic and linoleic acid levels using gas chromatography. Results demonstrated that the average mean of oleic and linoleic acid contents in high oleic acid genotypes were 72% and 17% respectively, while the average mean of oleic acid and linoleic acid contents in wild type lines were 33.5 % and 54 % respectively. These results indicated a perfect negative correlation between the amount of oleic and linoleic acids possessed in high and low oleic acid genotypes (R2 = -99.16%). Sequence characterised amplified region (SCAR) markers were tested to ascertain if any of the ten available dominant FAD2-1 markers was segregating with the high oleic acid allele. Four dominant SCAR markers (FAD2-1F4/R1; FAD2-1F4/R2; FAD2-1F13/R1; FAD2-1F14/R2) were strongly associated with the high oleic acid trait (P< 0.001). With regard to the inheritance of the high oleic acid trait, 143 plants of the F2 segregating population derived from a cross between the high oleic acid parent (AP901-95-3-4-1) and low oleic acid parent (H55-9-2-1-1) were genotyped with the four SCAR markers to determine the genetic state concerning the high oleic acid gene (Ol). Results from a Chi square analysis of the observed frequencies of each dominant FAD2-1 marker locus in 143 F2 individuals indicated that the deviation from the expected ratio of 3:1 (high to low oleic acid) was not statistically significant (P< 0.95) from the observed segregation ratio. These results were consistent with the previous finding that an incomplete dominant gene governs sunflower high oleic acid. A multiplex assay of 78 Simple sequence repeat (SSR) markers was optimised and evaluated on 143 plants of the F2 population to determine suitable SSR markers that can be used in a marker-assisted background selection. Only 14 markers were suitable for marker-assisted background selection based on their high polymorphic information content, allele frequency and maximum allele numbers. In conclusion, this study demonstrated the potential of using SSR and SCAR marker systems as a breeding tool to characterise and speed up the selection process in marker-assisted backcross breeding.Thesis (MSc (Botany))--North-West University, Potchefstroom Campus, 2013.
9
Nepraš, Ondřej. "Aplikace Lean Production." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2011. http://www.nusl.cz/ntk/nusl-229974.
Повний текст джерелаАнотація:
This master’s thesis deals with analysis and follow-up suggestion of efficiency improvement on the production line in RACIO s.r.o. First part of thesis is dealing with theoretical preparation and understanding lean production. Second part is dealing with analyzing production process and application of methods lean production. According to the production process analysis at the given production line a new solution of the production process has been suggested to increase production efficiency. This solution has been implemented and compared to the production process before, as well as to the production process after increasing the efficiency.
10
Benda, Ondřej. "Optimalizace činnosti měrového střediska." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2008. http://www.nusl.cz/ntk/nusl-228196.
Повний текст джерелаАнотація:
This master's thesis is engaged in optimisation processes of PUQ (as for Purchasing Quality) department, Robert Bosch České Budějovice. This department is responsible for input control of parts for manufacture as well as check of new parts including their documentation and documentation updates. By optimisation we understand selection of the best variant from group of possibilities.
Книги з теми "Scat DNA"
1
Bloemers, Peter. Schat uit de keuken van een Duits kasteel: De ontdekking van het DNA, 1869-2007. Nijmegen: Valkhof Pers, 2007.
Знайти повний текст джерела
2
Fox, Bob. A spiritual history of Tidewater, Virginia: The womb of America : a diagnostic scan of the spiritual DNA of America as seen in the first permanent British colony in North America, founded in 1607 at Jamestown, Virginia. Virginia Beach, VA: Bob Box, 2004.
Знайти повний текст джерела
3
YANTO, I. KADEK YOGI, I. PUTU SUARMA WIDIADA, NI KOMANG NARMINI, I. GEDE ANTO, I. KADEK AGUS, and I. GEDE BINTANG ARYA BUDAYA. Consciousness Trash Scan Sebagai Media Pembelajaran dalam Upaya Peningkatan Kesadaran Memilah dan Membuang Sampah Pada Anak Usia 5-8 Tahun. Baswara Press, 2023. http://dx.doi.org/10.53638/bp.08092023.001.
Повний текст джерелаАнотація:
Sampah menjadi permasalahan sulit diatasi di Indonesia, termasuk di Provinsi Bali dengan populasi 4.467.7 juta penduduk yang menyebabkan tingginya produksi sampah mencapai 915,5 ribu ton pada tahun 2021. Untuk mengatasi hal tersebut, dilaksanakan program edukasi berbasis masyarakat bernama Consciousness Trash Scan (CTS). Metode pengabdian melibatkan diskusi dengan TK Singasari I untuk menyusun jadwal kegiatan, pengembangan dan pengujian CTS dengan sampah organik dan non-organik, serta observasi untuk mengamati kemampuan anak-anak dalam memilah sampah. Proses implementasi alat dilakukan dengan cara memberikan kesempatan kepada masing-masing siswa untuk menggunakan CTS. Hasil analisis menunjukkan tingkat keberhasilan yang tinggi, dengan anak menjadi lebih antusias dan terampil memilah sampah (62%), mengidentifikasi jenis sampah dengan benar (59%), dan memahami pentingnya sampah (64%). Dampak positif program meluas ke rumah mereka (69%) dan dianggap dapat ditiru di tempat lain (65%). Hasil observasi melibatkan 49 anak menunjukkan bahwa seluruhnya, atau 100%, telah memiliki kemampuan yang baik dalam membedakan jenis sampah organik atau anorganik. Dengan dukungan positif ini, program CTS berhasil memberikan pemahaman yang bermanfaat tentang pengelolaan sampah pada anak-anak.
4
At home DNA tests: Marketing scam or medical breakthrough : hearing before the Special Committee on Aging, United States Senate, One Hundred Ninth Congress, second session, Washington, DC, July 27, 2006. Washington: U.S. G.P.O., 2006.
Знайти повний текст джерела
5
Torgerson,, Paul R., C. N. L. Macpherson, and D. A. Vuitton. Cystic echinococcosis. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0060.
Повний текст джерелаАнотація:
Cystic echinococcosis (CE)\cystic hydatid disease is one of the most widespread and important global helminth zoonoses. The parasite Echinococcus granulosus is maintained in a wide spectrum of intermediate hosts, including sheep, goats, camels, cattle, pigs and equines. A number of wild intermediate hosts occur, including cervids in the northern part of the North American continent and Eurasia, marsupials in Australia and wild herbivores in East and southern Africa. The application of a range of molecular techniques to the characterization of the parasite has confirmed the existence of mostly host-adapted strains and genotypes of the parasite and several new species have been proposed. The ubiquitous domestic dog serves as the most important definitive host for the transmission of the parasite throughout its wide geographical range.A wide range of diagnostic techniques, including necropsy, arecoline purgation, coproantigen ELISA and DNA based tests are available for detecting E. granulosus infection in the definitive host. In intermediate animal hosts, diagnosis at post mortem still remains the most reliable option. In humans, imaging techniques including ultrasound, nuclear magnetic resonance (NMR) or computer aided tomography (CAT-scan provide not only a method of diagnosis but also reveal important clinical information on the location, condition, number and size of the hydatid cysts in man. Of these ultrasound is the most widely used diagnostic technique and is the only imaging technique for screening of populations in rural areas, where the disease is most common. A classification system has been developed which can be used to assess the likely development of a cyst and hence guide the clinician in treatment options for the patient. Treatment relies on surgery and/or percutaneous interventions, especially ‘Puncture, Aspiration, Injection, Re-aspiration’ (PAIR) and/or antiparasitic treatment with albendazole (and alternatively mebendazole).CE is largely a preventable disease. Successful elimination programmes have focused on frequent periodic treatments of dogs with anthelmintics and the control of slaughter of domestic livestock. In many regions elimination or even control remains a problem as the parasite is endemic over vast areas of low income countries where there may be limited resources for control. In some areas, such as former communist administered countries, the parasite is resurgent. New tools are becoming available to control the parasite, including a highly effective vaccine in sheep which prevents the infection in sheep and breaks the transmission cycle. In addition cost effective methods are being developed which may be appropriate in low income countries where financial resources are not available for intensive control programmes that have been successful in high income countries.
Частини книг з теми "Scat DNA"
1
Glaz, Joseph, Joseph Naus, and Sylvan Wallenstein. "Scan Statistics in DNA and Protein Sequence Analysis." In Scan Statistics, 81–96. New York, NY: Springer New York, 2001. http://dx.doi.org/10.1007/978-1-4757-3460-7_6.
Повний текст джерела
2
Schbath, Sophie, and Stéphane Robin. "How Can Pattern Statistics Be Useful for DNA Motif Discovery?" In Scan Statistics, 319–50. Boston, MA: Birkhäuser Boston, 2009. http://dx.doi.org/10.1007/978-0-8176-4749-0_15.
Повний текст джерела
3
Leung, Ming-Ying, and Traci E. Yamashita. "Applications of the Scan Statistic in DNA Sequence Analysis." In Scan Statistics and Applications, 269–86. Boston, MA: Birkhäuser Boston, 1999. http://dx.doi.org/10.1007/978-1-4612-1578-3_12.
Повний текст джерела
4
Niemeyer, Charlotte M., and Brigitte Strahm. "Allogeneic Hematopoietic Cell Transplantation in Pediatric MDS Including Refractory Cytopenia of Childhood and in Juvenile Myelomonocytic Leukemia." In The EBMT Handbook, 679–83. Cham: Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-44080-9_75.
Повний текст джерелаАнотація:
AbstractPediatric MDS can be associated with germline predisposition, related to cytotoxic or immunmodulatory therapy, or occurs as de novo disease. SCT strategy is primarily dependent on blast count, karyotype, molecular abberrations, and BM cellularity. Juvenile myelomonocytic leukemia is a very heterogenous disease, not all children require SCT. Risk factors for relapse following SCT include age, HbF level, presence of secondary mutations and DNA methylation class.
5
Fu, James C., W. Y. Wendy Lou, and S. C. Chen. "On the Probability of Pattern Matching in Nonaligned DNA Sequences: A Finite Markov Chain Imbedding Approach." In Scan Statistics and Applications, 287–302. Boston, MA: Birkhäuser Boston, 1999. http://dx.doi.org/10.1007/978-1-4612-1578-3_13.
Повний текст джерела
6
Bahonar, Sajedeh, and Hesam Montazeri. "Somatic Single-Nucleotide Variant Calling from Single-Cell DNA Sequencing Data Using SCAN-SNV." In Variant Calling, 267–77. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2293-3_17.
Повний текст джерела
7
Serradell, L., J. M. Dupont, F. Ferr�, D. Rabineau, and P. Cornet. "Fetal Cell Detection in Maternal Blood Using the Solid Phase Cytometer Chem Scan®:RDI: Preliminary Studies." In Fetal Cells and Fetal DNA in Maternal Blood, 82–90. Basel: KARGER, 2001. http://dx.doi.org/10.1159/000062523.
Повний текст джерела
8
Cremer, Christoph, Michael Hausmann, Eduardo Diaz, Jutta Hetzel, Jacob A. Aten, and Thomas Cremer. "Chromosome Aberration Detection with Hybridized DNA Probes: Digital Image Analysis and Slit Scan Flow Cytometry." In Automation of Cytogenetics, 123–32. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-74738-0_11.
Повний текст джерела
9
Hellmeier, Joschka, René Platzer, Johannes B. Huppa, and Eva Sevcsik. "A DNA Origami-Based Biointerface to Interrogate the Spatial Requirements for Sensitized T-Cell Antigen Recognition." In The Immune Synapse, 277–302. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3135-5_18.
Повний текст джерелаАнотація:
AbstractWhen T cells scan the surface of antigen-presenting cells (APCs), they can detect the presence of just a few antigenic peptide/MHC complexes (pMHCs), in some cases even a single agonist pMHC. These are typically vastly outnumbered by structurally similar yet non-stimulatory endogenous pMHCs. How T cells achieve this enormous sensitivity and selectivity is still not clear, in particular in view of the rather moderate (1–100 μM) affinity that T-cell receptors (TCRs) typically exert for antigenic pMHCs. Experimental approaches that enable the control and quantification of physical input parameters within the context of the immunological synapse to precisely interrogate the molecular consequences of TCR-engagement, appear highly advantageous when searching for better answers.We here describe the implementation of a biointerface that allows to experimentally define molecular distances between T-cell ligands as a means to correlate them with molecular dynamics of antigen engagement, downstream signaling, and the overall T-cell response. The basis of this biointerface is DNA origami nanostructures, which are (i) rigid and highly versatile platforms that can (ii) be embedded as laterally mobile entities within supported lipid bilayers and functionalized (iii) in a site-specific and orthogonal manner with (iv) one or more proteins of choice.
10
Anwar, Suzan, Mardin Anwer, and Daniah Al-Nadawi. "DeepFake Technology for Breast Cancer Dataset Generation Using Autoencoders and Deep Neural Networks." In Lecture Notes in Computer Science, 3–21. Cham: Springer Nature Switzerland, 2025. https://doi.org/10.1007/978-3-031-88220-3_1.
Повний текст джерелаАнотація:
Abstract In the emerging field of radiogenomics, the primary challenge is the high cost of genetic testing, which restricts access to large, paired datasets of imaging and genetic information. Such datasets are essential for the effective training of machine learning algorithms in radiogenomic analyses. This research aims to bridge the gap between gene expression in tumors and their morphological representation in MRI scans of breast cancer patients. In this work an advanced autoencoder for processing gene expression data, and the derived weights from this autoencoder utilized were then employed to initialize a supervised Deep Neural Network (DNN). This network extracted distinct morphological markers from each MRI scan . This study introduces an innovative approach that utilizes deepfake technology, employing dual Generative Adversarial Networks (GANs) to generate synthetic imaging data from a radiogenomic dataset. This synthetic data, nearly indistinguishable from real data, is produced using a supervised neural network and is aimed at enhancing breast cancer diagnostics. Notably, the proposed neural network, when enhanced with an autoencoder and dropout techniques, demonstrated superior predictive accuracy over linear regression models. Specifically, it reduced errors by an average of 1.8% in mean absolute percent error. These findings underscore that the images generated by the proposed model are virtually indistinguishable from authentic images and exhibit high reliability in applications through the PyTorch framework. The results of this study underscore the potential of the proposed methodology to significantly contribute to advancements in breast cancer diagnostics.
Тези доповідей конференцій з теми "Scat DNA"
1
Stevens, Michael G. "Accelerated FRP Corrosion Testing Using Dynamic Mechanical Analysis." In CORROSION 2003, 1–14. NACE International, 2003. https://doi.org/10.5006/c2003-03614.
Повний текст джерелаАнотація:
Abstract The industry standard for evaluating the suitability of a fiber-reinforced composite (FRP) has been the ASTM C-581 test procedure. In this test, a coupon is made to simulate the corrosion barrier. Several sample coupons are then immersed into the solution that is being evaluated at the desired test temperature and tested at 1 month, 3 month, 6 month, and 12 month intervals. The tests run are flexural strength, flexural modulus and surface hardness. A new way for evaluating the test coupons is being evaluated and will be discussed in this paper. Dynamic Mechanical Analysis (DMA) is a test that is non-destructive and can be run on the same test coupon over the entire test period. The samples can be pulled, tested and then placed back into the test solution. This may eliminate the variability that is seen between the coupons. Another advantage to using DMA is that a modulus versus temperature scan can be run on the sample that will give information on the type of attack that is occurring. This may also be able to predict if a resin system is suitable for a chemical environment at an earlier time in the test such as 3 or 6 months instead of 12 months.
2
Eisenlord, S., A. Darzins, Carrie Keller-Schultz, and Victor V. Keasler. "Evaluation of Propidium Monoazide as a Tool to Differentiate Live from Dead MIC Microorganisms." In CORROSION 2016, 1–15. NACE International, 2016. https://doi.org/10.5006/c2016-07347.
Повний текст джерелаАнотація:
Abstract We describe the advancement of an activity-based qPCR assay which can distinguish live from dead corrosion influencing microorganisms in oil and gas pipeline environments. While the current body of knowledge is expanding for the types of microbial organisms present in waters used for natural gas and oil production, there is scant information on the activity of these potentially deleterious or beneficial organisms in source waters and produced waters associated with the industry. This assay was developed using pure cultures of sulfate reducing bacteria (SRB), sulfate reducing Archaea (SRA), iron oxidizing bacteria (IOB), denitrifying bacteria (DNB), and archaea (AR). Propidium monoazide-qPCR was then adapted to differentiate live from dead bacteria Pseudomonas putida bacterial cells once immobilized on membrane filters. The assay has been validated with mock environmental samples, where produced waters of various turbidity and origin have been sterilized and spiked with known quantities of living and dead P.putida. We discuss the limitations and possible future optimization methods for Propidium monazide-qPCR techniques in the industry.
3
Dancus, I., V. I. Vlad, A. Petris, I. Rau, F. Kajzar, A. Meghea, and A. Tane. "Nonlinear optical properties of Rh610 sensitized DNA-CTMA characterized by Z-Scan." In ROMOPTO International Conference on Micro- to Nano-Photonics III, edited by Valentin I. Vlad. SPIE, 2013. http://dx.doi.org/10.1117/12.2032352.
Повний текст джерела
4
Ye, Jing, Yu Huang, Yu Hu, Wu-Tung Cheng, Ruifeng Guo, Liyang Lai, Ting-Pu Tai, et al. "Diagnosis and Layout Aware (DLA) scan chain stitching." In 2013 IEEE International Test Conference (ITC). IEEE, 2013. http://dx.doi.org/10.1109/test.2013.6651929.
Повний текст джерела
5
Setyawati, Tri Rima, Rikhsan Kurniatuhadi, and Ari Hepi Yanti. "Karakter Morfologi Koloni Streptomyces Spp. yang diisolasi dari Substrat Habitat Cacing Nipah (Namalycastis Rhodochorde) pada Medium Berbeda." In Seminar Nasional Penerapan Ilmu Pengetahuan dan Teknologi : kampus merdeka meningkatkan kecerdasan sumberdaya manusia melalui interdispliner ilmu pengetahuan dan teknologi : Pontianak, 24 Agustus 2021. Untan Press, 2021. http://dx.doi.org/10.26418/pipt.2021.29.
Повний текст джерелаАнотація:
Streptomyces memiliki potensi sebagai sumber metabolit antibakteri yang dapat dikembangkan dan diaplikasikan dalam pengembangan budidaya cacing nipah. Streptomyces memiliki karakter morfologi kultur yang berbeda-beda di beberapa medium agar dan merupakan aspek yang sangat penting dalam proses identifikasi dan klasifikasinya. Streptomyces spp. telah diisolasi dari substrat alami habitat cacing nipah (Namalycastis rhodochorde) di Sungai Kakap Kabupaten Kubu Raya sebanyak enam isolat (NrASA1 – NrASA6) dan telah berhasil dimurnikan untuk dilakukan karakterisasi morfologi koloninya. Penelitian ini bertujuan untuk mengkarakterisasi variasi koloni Streptomyces sehingga diketahui variasi koloni dari genus yang sama pada medium yang berbeda. Karakterisasi dilakukan dengan metode pengamatan langsung terhadap koloni Streptomyces yang dikultur secara kuadran pada medium agar glycerol asparagine (GAA), inorganic salt starch (ISSA), oatmeal (OA), dan starch casein (SCA). Hasil karakterisasi tujuh isolat Streptomyces spp. yang dikultur pada empat medium agar berbeda memperlihatkan bahwa persamaan karakter terlihat dari warna spora matang yang 100% berwarna coklat, interval diameter koloni antara 3 – 8 cm, tekstur bersifat cottony. Perbedaan karakter terlihat dari presensi diffusible pigment yang hanya ada pada koloni isolat NrASA1 dan NrASA3 pada medium GAA dan SCA, sedangkan presensi eksudat berwarna coklat tua hanya terjadi pada medium ISSA. Enam isolat Streptomyces diduga memiliki epitet spesies yang berbeda, khususnya antara NrASA1 dan NrASA3 terhadap kode isolat lainnya.
6
Zhang, Yan, and Albert Ratner. "Fuel Drop Spreading on a Flat Smooth Surface: Numerical Simulations With a VOF Method." In ASME 2010 International Mechanical Engineering Congress and Exposition. ASMEDC, 2010. http://dx.doi.org/10.1115/imece2010-39411.
Повний текст джерелаАнотація:
With the current interest in bio-derived and blended transportation fuels, the impact of the variable viscosity in these fuels on spray and splash properties has become an area of concern. In this work, the dynamics of a liquid drop impacting and spreading on a flat, smooth surface was computationally investigated by employing the volume of fluid (VOF) approach with the commercial solver Fluent 12.0.16, and the results were base-lined with experimental measurements. Of particular interest was the degree of fidelity required of the contact angle model, with the present work proposing and testing a combined static contact angle-dynamic contact angle (SCA-DCA) model to describe drop spreading. This model was shown to reduce the behavior information required as compared with full dynamic contact angle (DCA) models while significantly improving over the accuracy of a pure static contact angle (SCA) model. Two different computational domains were tested and compared for the proposed SCA-DCA model, a quarter-drop versus a full-drop domain, with the results showing that the error was reduced when the full domain was employed.
7
Ma, Suihua, Le Liu, Weiping Lu, Yaou Zhang, Yonghong He, and Jihua Guo. "Study on the SPR responses of various DNA probe concentrations by parallel scan spectral SPR imaging." In Photonics and Optoelectronics Meetings, edited by Qingming Luo, Lihong V. Wang, and Valery V. Tuchin. SPIE, 2008. http://dx.doi.org/10.1117/12.823908.
Повний текст джерела
8
Corti, A., L. Lombardi, and G. Manfrida. "Absorption of CO2 With Amines in a Semiclosed GT Cycle: Plant Performance and Operating Costs." In ASME 1998 International Gas Turbine and Aeroengine Congress and Exhibition. American Society of Mechanical Engineers, 1998. http://dx.doi.org/10.1115/98-gt-395.
Повний текст джерелаАнотація:
A CO2 removal system, using aqueous solutions of amines, is applied to a Semi-Closed Gas Turbine/Combined Cycle (SCGT/CC) power plant. The SCGT/CC is interesting because of the possibility of achieving low emissions at the stack, with a decreased overall flow rate, lower NOx concentration and an increased CO2 concentration (over 15% in volume), which facilitates the removal treatment. Several compositions of the absorbing solution have been investigated, by means of simulations with ASPEN PLUS. A 18% DEA + 12% MDEA composition resulted the most convenient in terms of flow rate and energy requirement for the stripping. A cost analysis of the removal plant allows to estimate additional costs for CO2 removal with respect to conventional power plants.
9
Abdisalaam, Salim, Milan Poudel, David Chen, and George Alexandrakis. "Quantifying kinetics and dynamics of DNA repair proteins using Raster-scan Image Correlation Spectroscopy and Fluorescence Recovery after Photobleaching." In Bio-Optics: Design and Application. Washington, D.C.: OSA, 2011. http://dx.doi.org/10.1364/boda.2011.jtua5.
Повний текст джерела
10
Reynolds, L. M., C. D. Langefeld, A. C. Bishop, T. D. Howard, E. R. Bleecker, G. A. Hawkins, I. Barjaktarevic, et al. "Differential DNA Methylation in Lower Airways Epithelial Cells Associate with Lung Function and CT Scan-Based Lung Structure in SPIROMICS." In American Thoracic Society 2022 International Conference, May 13-18, 2022 - San Francisco, CA. American Thoracic Society, 2022. http://dx.doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a4678.
Повний текст джерелаЗвіти організацій з теми "Scat DNA"
1
Glasscott, Matthew, Johanna Jernberg, Erik Alberts, and Lee Moores. Toward the electrochemical detection of 2,4-dinitroanisole (DNAN) and pentaerythritol tetranitrate (PETN). Engineer Research and Development Center (U.S.), March 2022. http://dx.doi.org/10.21079/11681/43826.
Повний текст джерелаАнотація:
Analytical methods to rapidly detect explosive compounds with high precision are paramount for applications ranging from national security to environmental remediation. This report demonstrates two proof-of-concept electroanalytical methods for the quantification of 2,4-dinitroanisol (DNAN) and pentaerythritol tetranitrate (PETN). For the first time, DNAN reduction was analyzed and compared at a bare graphitic carbon electrode, a polyaniline-modified (PANI) electrode, and a molecularly imprinted polymer (MIP) electrode utilizing PANI to explore the effect of surface-area and preconcentration affinity on the analytical response. Since some explosive compounds such as PETN are not appreciably soluble in water (<10 μg/L), necessitating a different solvent system to permit direct detection via electrochemical reduction. A 1,2-dichloroethane system was explored as a possibility by generating a liquid-liquid extraction-based sensor exploiting the immiscibility of 1,2-dichloroethane and water. The reduction process was explored using a scan rate analysis to extract a diffusion coefficient of 6.67 x 10⁻⁶ cm/s, in agreement with literature values for similarly structured nitrate esters. Once further refined, these techniques may be extended to other explosives and combined with portable electrochemical hardware to bring real-time chemical information to soldiers and citizens alike.
2
Sadka, Avi, Mikeal L. Roose, and Yair Erner. Molecular Genetic Analysis of Citric Acid Accumulation in Citrus Fruit. United States Department of Agriculture, March 2001. http://dx.doi.org/10.32747/2001.7573071.bard.
Повний текст джерелаАнотація:
The acid content of the juice sac cells is a major determinant of maturity and fruit quality in citrus. Many citrus varieties accumulate acid in concentrations that exceed market desires, reducing grower income and consumer satisfaction. Pulp acidity is thought to be dependent on two mechanisms: the accumulation of citric acid in the vacuoles of the juice sac cells, and acidification of the vacuole. The major aim of the project was to direct effort toward understanding the mechanism of citric acid accumulation in the fruit. The following objectives were suggested: Measure the activity of enzymes likely to be involved in acid accumulation and follow their pattern of expression in developing fruit (Sadka, Erner). Identify and clone genes which are associated with high and low acid phenotypes and with elevated acid level (Roose, Sadka, Erner). Convert RAPD markers that map near a gene that causes low acid phenotype to specific co dominant markers (Roose). Use genetic co segregation to test whether specific gene products are responsible for low acid phenotype (Roose and Sadka). Objective 1 was fully achieved. Most of the enzymes of organic acid metabolism were cloned from lemon pulp. Their expression was studied during fruit development in low and high acid varieties. The activity and expression of citrate synthase, aconitase and NADP-isocitrate dehydrogenase (IDH) were studied in detail. The role that each enzyme plays in acid accumulation and decline was evaluated. As a result, a better understanding of the metabolic changes that contribute to acid accumulation was achieved. It was found that the activity of the mitochondrial aconitase is greatly reduced early in high-acid fruits, but not in acidless ones, suggesting that this enzyme plays an important role in acid accumulation. In addition, it was demonstrated that increases in the cytosolic forms of aconitase and NADP-IDH towards fruit maturation play probably a major role in acid decline. Our studies also demonstrated that the two mechanisms that contribute to fruit acidity, vacuolar acidification and citric acid accumulation, are independent, although they are tightly co-regulated. Additional, we demonstrated that sodium arsenite, which reduce fruit acidity, causes a transient inhibition in the activity of citrate synthase, but an induction in the gene expression. This part of the work has resulted in 4 papers. Objective 3 was also fully achieved. Using bulked segregant analysis, three random amplified polymorphic DNA (RAPD) markers were identified as linked to acitric, a gene controlling the acidless phenotype of pummelo 2240. One of them, which mapped 1.2 cM from acitric was converted into sequence characterized amplified region (SCAR marker, and into co dominant restriction length polymorphism (RFLP) marker. These markers were highly polymorphic among 59 citrus accessions, and therefore, they should be useful for selecting seedling progeny heterozygous for acitric in nearly all crosses between pummelo 2240 and other citrus genotypes. This part of the project resulted in one paper. Objective 4 was also fully achieved. Clones isolated by the Israeli group were sent to the American laboratory for co segregation analysis. However, none of them seemed to co segregate with the low acid phenotype. Both laboratories invested much effort in achieving the goals of Objective 2, namely the isolation of genes that are elevated in expression in low and high acid phenotypes, and in tissue cultures treated with arsenite (a treatment which reduces fruit acidity). However, conventional differential display and restriction fragment differential display analyses could not identify any differentially expressed genes. The isolation of such genes was the major aim of a continuation project, which was recently submitted.