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Статті в журналах з теми "Stress Induced Isozyme"

Автор: Grafiati

Опубліковано: 3 червня 2025

Оновлено: 31 липня 2025

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1

Miller, A. Raymond, and Thomas J. Kelley. "Mechanical Stress Stimulates Peroxidase Activity in Cucumber Fruit." HortScience 24, no. 4 (1989): 650–52. http://dx.doi.org/10.21273/hortsci.24.4.650.

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Abstract Peroxidase activity was determined in cucumber fruit (37 to 55 mm in diameter) subjected to mechanical stress followed by storage at 25C and 100% RH. Compared to unstressed, unstored control fruit, severe mechanical stress stimulated total extractable peroxidase activity after 24 and 48 hr of storage. Moderate mechanical stress or storage alone stimulated peroxidase activity to lesser degrees. Peroxidase isozyme analysis by native polyacrylamide gel electrophoresis showed that cucumber anodic peroxidases could be separated into slow-, moderate-, and fast-migrating groups. Both moderat

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2

Huttová, J., MistríkI, M. Ollé-Šimonovičová, and L. Tamás. "Cadmium induced changes in cell wall peroxidase isozyme pattern in barley root tips." Plant, Soil and Environment 52, No. 6 (2011): 250–53. http://dx.doi.org/10.17221/3437-pse.

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Changes in peroxidase activity as well as isozyme peroxidase pattern were analyzed in five cell wall (CW) fractions of barley roots exposed to 1mM CdCl<sub>2</sub>for 48 and 72 h. Whereas strong inhibition of peroxidase activity was detected in fraction CW I and weak inhibition in fractions CW II, III and IV, strong activation of peroxidase was observed in fraction V after Cd application. Despite the inhibition of enzyme activity in most CW fractions, induction of several isoperoxidases was discovered after separation on PAGE. In fraction CW I inhibition of one cathodic isoperoxida

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3

He, Yali, and Bingru Huang. "Differential Responses to Heat Stress in Activities and Isozymes of Four Antioxidant Enzymes for Two Cultivars of Kentucky Bluegrass Contrasting in Heat Tolerance." Journal of the American Society for Horticultural Science 135, no. 2 (2010): 116–24. http://dx.doi.org/10.21273/jashs.135.2.116.

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Understanding antioxidant mechanisms for heat stress is important for improving heat tolerance in cool-season plant species. The objective of this study was to identify antioxidant enzymes associated with cultivar variations in heat tolerance in kentucky bluegrass (Poa pratensis) by comparing heat responses of activity and isoforms of antioxidant enzymes in two cultivars contrasting in heat tolerance. Plants of heat-tolerant ‘Eagleton’ and heat-sensitive ‘Brilliant’ were exposed to 20 °C (control) or 40 °C (heat stress) for 28 days in growth chambers. Chlorophyll (Chl) a content remained uncha

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4

Tamás, L., J. Huttová, I. Mistrík, M. Šimonovičová, and B. Široká. "Aluminium induced esterase activity and isozyme pattern in barley root tip." Plant, Soil and Environment 51, No. 5 (2011): 220–25. http://dx.doi.org/10.17221/3577-pse.

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Changes in the activity of esterase as well as changes in the viability of root cells and some growth parameters were analysed during cultivation of barley seedlings in the artificial substrate under Al stress conditions. Aluminium-induced elevated esterase activity correlated with Al uptake, root growth inhibition and increased Evans blue uptake in the barley root tips. Analysis of isozyme pattern of esterase revealed one anodic and one cathodic esterase isozyme induced by Al-treatment. The possible role of elevated esterase activity during Al stress is discussed.

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5

Pan, Guodong, Mandar Deshpande, Rajarajan A. Thandavarayan, and Suresh Selvaraj Palaniyandi. "ALDH2 Inhibition Potentiates High Glucose Stress-Induced Injury in Cultured Cardiomyocytes." Journal of Diabetes Research 2016 (2016): 1–8. http://dx.doi.org/10.1155/2016/1390861.

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Aldehyde dehydrogenase (ALDH) gene superfamily consists of 19 isozymes. They are present in various organs and involved in metabolizing aldehydes that are biologically generated. For instance, ALDH2, a cardiac mitochondrial ALDH isozyme, is known to detoxify 4-hydroxy-2-nonenal, a reactive aldehyde produced upon lipid peroxidation in diabetic conditions. We hypothesized that inhibition of ALDH leads to the accumulation of unmetabolized 4HNE and consequently exacerbates injury in cells subjected to high glucose stress. H9C2 cardiomyocyte cell lines were pretreated with 10 μM disulfiram (DSF), a

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6

Almas, Semra, and Tijen Demiral Sert. "Metal Sulfates and Microbial Food Treatment Alleviate the Oxidative Damage Caused by PEG-Induced Osmotic Stress in Cotton Plants." Advancements in Life Sciences 11, no. 3 (2024): 572. http://dx.doi.org/10.62940/als.v11i3.1739.

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Background: Drought stress restricts cotton production, which causes a considerable loss of cotton yields worldwide. Plant activators improve crop production and ameliorate abiotic stresses. Thus, the effects of plant activators on the genotype of cotton (Gossypium hirsutum L.) Candia, which is resistant to drought, were investigated.Methods: Plants were treated with the plant activators, and then osmotic stress was initiated by 30% PEG 6000 treatment for 13 days. Antioxidant enzyme activities such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and peroxidase (POX) were measured al

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7

Jiang, Shun Yao, and Pei Jiang Zhou. "Effects of Cadmium on the Expression of Antioxidant Enzymes, Oxidative Stress and Apoptosis in Primary Hepatocytes of Carassius Auratus." Advanced Materials Research 518-523 (May 2012): 341–46. http://dx.doi.org/10.4028/www.scientific.net/amr.518-523.341.

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The primary hepatocytes of Carassius auratus were incubated with 0, 0.01, 0.1, 1, 10 and 100 mg/l Cadmium(Cd2+) at 25°C for 8h in vitro. The results showed that the hepatocytes survival rate in the 1, 10 and 100 mg/l Cd2+ treated groups were significantly lower than that in the control, the percentage of apoptotic hepatocytes significantly increased in 0.1, 1 and 10 mg/l of Cd2+ treated groups, intracellular reactive oxygen species(ROS) and malondialdehyde (MDA) content significantly increased in 0.1, 1, 10 and 100 mg/l Cd2+ treated groups, typical DNA ladder was observed in 0.1, 1 and 10 mg/l

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8

Ying, X. F., P. Liu, and G. D. Xu. "Effect of aluminum on the isozymes of the seedlings of two soybeans [Glycine max (L.) Merrill] varieties." Plant, Soil and Environment 52, No. 6 (2011): 262–70. http://dx.doi.org/10.17221/3439-pse.

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Aluminum (Al) is not the necessary nutrient but the most abundant metal in the earth&rsquo;s crust. Isozymes of the <br />seedlings of two soybean varieties (Zhechun No. 3 and Huachun No. 18) and their response to different Al treatments had been analyzed. The study showed that the responses to Al toxicity of different isozymes were quite different. Among these four isozymes, esterase (EST) isozyme was the least sensitive to Al toxicity. There was no distinct difference in the zymograms of EST under different Al treatments. Al toxicity enhanced the activity of peroxidase (POD) and in

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9

Vishnu, Priya S., and P. Somasundaram. "INDUCED PHENOLOXIDASE PROFILES IN SILKWORM Bombyx mori (L.) UNDER BIOTIC STRESS." International Journal of Engineering Technologies and Management Research 4, no. 10 (2017): 46–52. https://doi.org/10.5281/zenodo.1042479.

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<strong><em>Phenol oxidase (PO) is one of the stress enzyme protein in living organism. The conversion of Pro-PO into an activation form of PO required a stress protein. In the present study has emerged with the novel finding of induced phenoloxidase was identified under bacterial endotoxin viz., Lipopolysaccharide (LPS) activity using silkworm Bombyx mori as an animal model. The PO enzyme plays an important role for insect survival during pathophysiological conditions. The enzyme activity was analyzed from ten different silkworm races with two phenolic substrates viz., L-Dopa and Dopamine by

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10

EL-BELTAGI, Hossam Saad, Amal A. MOHAMED, and Mohamed M. RASHED. "Response of Antioxidative Enzymes to Cadmium Stress in Leaves and Roots of Radish (Raphanus sativus L.)." Notulae Scientia Biologicae 2, no. 4 (2010): 76–82. http://dx.doi.org/10.15835/nsb245395.

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Presented study has demonstrated that exposure of plants to toxic heavy metal Cd results a reduction in plant growth. Varied concentrations of CdCl2, ranging from 0.0 to 50 ppm in the germinating media reduced leaf area of radish plant, chlorophyll and carotenoid contents. Greater loss of chlorophyll b content than chlorophyll a was observed especially under 50 ppm Cd exposure. With regards to the distribution of Cd in roots and leaves, the obtained data showed that the maximum accumulation of Cd occurred in roots followed by leaves. Generally, Fe, Zn, Mn and Cu declined in leaves compared to

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11

Malhotra, Ashwani, Rebecca Begley, Barinder P. S. Kang та ін. "PKC-ε-dependent survival signals in diabetic hearts". American Journal of Physiology-Heart and Circulatory Physiology 289, № 4 (2005): H1343—H1350. http://dx.doi.org/10.1152/ajpheart.01200.2004.

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Diabetes mellitus is complicated by the development of a primary cardiomyopathy, which contributes to the excess morbidity and mortality of this disorder. The protein kinase C (PKC) family of isozymes plays a key role in the cardiac phenotype expressed during postnatal development and in response to pathological stimuli. Hyperglycemia is an activating signal for cardiac PKC isozymes that modulate a myriad of cell events including cell death and survival. The ε-isozyme of the PKC family transmits a powerful survival signal in cardiac muscle cells. Accordingly, to test the hypothesis that endoge

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12

Togane, Yuko, Toshisuke Morita, Makoto Suematsu, Yuzuru Ishimura, Jun-Ichi Yamazaki, and Shigehiro Katayama. "Protective roles of endogenous carbon monoxide in neointimal development elicited by arterial injury." American Journal of Physiology-Heart and Circulatory Physiology 278, no. 2 (2000): H623—H632. http://dx.doi.org/10.1152/ajpheart.2000.278.2.h623.

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We reported that carbon monoxide (CO) generated through heme oxygenase (HO) inhibits mitogen-induced proliferation of vascular smooth muscle cells (VSMCs). We report that balloon injury induces HO-1, the stress-inducible isozyme of HO, in VSMCs and inhibits neointimal formation through the action of endogenous CO. Northern blot analysis and immunohistochemistry revealed that HO-1 is markedly induced in the media as early as 1 day after injury, whereas only a little expression was detected in the intact carotid artery. The neointimal proliferative changes were augmented or inhibited by the HO i

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13

Liou, Ying-Ming, Meei Jyh Jiang, and Ming-Che Wu. "Altered Expression of Cardiac Myosin Isozymes Associated with the Malignant Hyperthermia Genotype in Swine." Anesthesiology 93, no. 5 (2000): 1312–19. http://dx.doi.org/10.1097/00000542-200011000-00026.

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Background Anesthetic-induced malignant hyperthermia (MH) in humans and pigs is associated with dramatic alterations in cardiac function. However, it remains controversial as to whether MH-associated cardiac symptoms represent a primary difference of myocardium or a secondary alteration consequent to increases in the hyperthermic stress. Here the authors describe changes in myosin isoform expression in the hearts of MH-susceptible pigs with and without prior exposure to halothane. Methods One group of pigs was diagnosed as MH susceptible by halothane challenge and Hal-1843 nucleotide examinati

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14

Zhou, Qian, Ayumi Suzuki, Mitsuo Iinuma, Ke-Yong Wang, Kin-ya Kubo, and Kagaku Azuma. "Effects of Maternal Chewing on Prenatal Stress-Induced Cognitive Impairments in the Offspring via Multiple Molecular Pathways." International Journal of Molecular Sciences 21, no. 16 (2020): 5627. http://dx.doi.org/10.3390/ijms21165627.

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We aimed to investigate the effects of maternal chewing on prenatal stress-induced cognitive impairments in the offspring and to explore the molecular pathways of maternal chewing in a mice model. Maternal chewing ameliorated spatial learning impairments in the offspring in a Morris water maze test. Immunohistochemistry and Western blot findings revealed that maternal chewing alleviated hippocampal neurogenesis impairment and increased the expression of hippocampal brain-derived neurotrophic factor in the offspring. In addition, maternal chewing increased the expression of glucocorticoid recep

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15

Huang, Yun, Shaoling Huang, Yue Wu, et al. "Lipoxygenase Protein Expression and Its Effect on Oxidative Stress Caused by Benzidine in Normal Human Urothelial Cell Lines." International Journal of Toxicology 38, no. 2 (2019): 121–28. http://dx.doi.org/10.1177/1091581819827495.

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Metabolic activation of indirect-acting carcinogens in the target organ is an effective mechanism of carcinogenesis. Lipoxygenase (LOX) can co-oxidize the bladder carcinogen benzidine (BZ). However, it is not entirely clear whether BZ is activated and which enzyme is involved in its activation in bladder epithelial cells. Our results showed that BZ induced 5-LOX protein expression but had no significant influence on the expression of 15-LOX-2, CYP1B1, and CYP2E1 in SV-40 immortalized human uroepithelial SV-HUC-1 cells. BZ induced oxidative stress in SV-HUC-1 cells by increasing reactive oxygen

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16

Sachan, Richa, Amit Kundu, Prasanta Dey, et al. "Dendropanax morbifera Protects against Renal Fibrosis in Streptozotocin-Induced Diabetic Rats." Antioxidants 9, no. 1 (2020): 84. http://dx.doi.org/10.3390/antiox9010084.

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The aquatic extract of Dendropanax morbifera (DP) is typically consumed as a beverage in Korea and China and is also used in various traditional medicines. However, the functional role of DP on diabetes-induced renal fibrosis is unclear. Here, the protective effects of DP extract against diabetes-induced renal fibrosis were evaluated. Streptozotocin (STZ, 60 mg/kg) was injected intraperitoneally in rats to induce diabetes. After 5 days, DP extract (25 mg/kg/day) and metformin (50 mg/kg/day) were administered orally to diabetic rats for 28 days. DP administration protected both body and organ w

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17

Bangaru Naidu, Thaddi. "Dissecting Salt Stress-Induced Proteomic Changes in Pigeonpea (Cajanus cajan L. Millspaugh) Leaves: A Detailed Analysis of Stress-Responsive Protein Dynamics." Research Journal of Biotechnology 20, no. 2 (2024): 122–33. https://doi.org/10.25303/202rjbt1220133.

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Salinity is a major abiotic stress that poses significant challenges to global agriculture by reducing crop productivity and soil fertility. In pigeonpea (Cajanus cajan L. Millspaugh), comprehensive studies investigating proteomic responses to salt stress are limited. This study aims to establish a detailed proteomic profile for two pigeonpea varieties: ICPL 87119 (salt-tolerant) and ICPL 85063 (salt-sensitive) under NaCl-induced salt stress. The study evaluated the effects of different NaCl concentrations (0, 50 mM, 100 mM, 150 mM and 200 mM) on seedling growth including parameters such as pl

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18

Fukuda, Aya, Toshihiko Osawa, Hiroaki Oda, Shinya Toyokuni, Kimihiko Satoh, and Koji Uchida. "Oxidative Stress Response in Iron-Induced Renal Carcinogenesis: Acute Nephrotoxicity Mediates the Enhanced Expression of GlutathioneS-Transferase Yp Isozyme." Archives of Biochemistry and Biophysics 329, no. 1 (1996): 39–46. http://dx.doi.org/10.1006/abbi.1996.0189.

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19

Abdelrahman, RS, MS El-Awady, MA Nader, and EM Ammar. "Hydrogen sulfide ameliorates cardiovascular dysfunction induced by cecal ligation and puncture in rats." Human & Experimental Toxicology 34, no. 10 (2015): 953–64. http://dx.doi.org/10.1177/0960327114564794.

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Hydrogen sulfide (H2S) is an endogenously produced gaseous messenger that participates in regulation of cardiovascular functions. This study evaluates the possible protective effect of H2S in cardiovascular dysfunction induced by cecal ligation and puncture (CLP) in rats. After 24 h of induction of CLP, heart rate (HR), mortality, cardiac and inflammation biomarkers (creatine kinase-MB (CK-MB) isozyme, cardiac troponin I (cTnI), C-reactive protein (CRP), and lactate dehydrogenase (LDH)), in vitro vascular reactivity, histopathological examination, and oxidative biomarkers (malondialdehyde (MDA

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20

Sreedevi, Sasidharan, Kulangara Nanu Remani, and Sailas Benjamin. "Biotic Stress Induced Biochemical and Isozyme Variations in Ginger and Tomato by <i>Ralstonia solanacearum</i>." American Journal of Plant Sciences 04, no. 08 (2013): 1601–10. http://dx.doi.org/10.4236/ajps.2013.48194.

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21

Kam, Yoonseok, and John H. Exton. "Phospholipase D Activity Is Required for Actin Stress Fiber Formation in Fibroblasts." Molecular and Cellular Biology 21, no. 12 (2001): 4055–66. http://dx.doi.org/10.1128/mcb.21.12.4055-4066.2001.

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ABSTRACT Phospholipase D (PLD) is a ubiquitously expressed enzyme of ill-defined function. In order to explore its cellular actions, we inactivated the rat PLD1 (rPLD1) isozyme by tagging its C terminus with a V5 epitope (rPLD1-V5). This was stably expressed in Rat-2 fibroblasts to see if it acted as a dominant-negative mutant for PLD activity. Three clones that expressed rPLD1-V5 were selected (Rat2V16, Rat2V25, and Rat2V29). Another clone (Rat2V20) that lost expression of rPLD1-V5 was also obtained. In the three clones expressing rPLD1-V5, PLD activity stimulated by phorbol myristate acetate

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22

Park, Hye Lin, Youngchul Yoo, Seong Hee Bhoo, Tae-Hoon Lee, Sang-Won Lee, and Man-Ho Cho. "Two Chalcone Synthase Isozymes Participate Redundantly in UV-Induced Sakuranetin Synthesis in Rice." International Journal of Molecular Sciences 21, no. 11 (2020): 3777. http://dx.doi.org/10.3390/ijms21113777.

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Chalcone synthase (CHS) is a key enzyme in the flavonoid pathway, participating in the production of phenolic phytoalexins. The rice genome contains 31 CHS family genes (OsCHSs). The molecular characterization of OsCHSs suggests that OsCHS8 and OsCHS24 belong in the bona fide CHSs, while the other members are categorized in the non-CHS group of type III polyketide synthases (PKSs). Biochemical analyses of recombinant OsCHSs also showed that OsCHS24 and OsCHS8 catalyze the formation of naringenin chalcone from p-coumaroyl-CoA and malonyl-CoA, while the other OsCHSs had no detectable CHS activit

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23

Mishko, A. E., and E. O. Lutskiy. "Dynamics in the activity of peroxidase and its isoforms in leaves of different apple cultivars." Proceedings on applied botany, genetics and breeding 182, no. 3 (2021): 37–43. http://dx.doi.org/10.30901/2227-8834-2021-3-37-43.

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Background. Various approaches are used for identification of the most resistant fruit crop cultivars, including the analysis of different physiological and biochemical indicators. In Krasnodar Territory, Russia, one of the major stressors in summer is the hydrothermal stress. Drought and heat lead to an oxidative stress, as reactive oxygen species are produced in plant cells. Plants respond to oxidative damage by activating antioxidant enzymes, such as superoxide dismutase, catalase, and various peroxidases. Peroxidase is able to decompose hydrogen peroxide. Peroxidase activity was calculated

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24

Karigane, Daiki, Keiyo Takubo, Shinichiro Okamoto та Toshio Suda. "p38α Is Required for Proper Proliferation of Hematopoietic Stem Cells during Stress". Blood 124, № 21 (2014): 4317. http://dx.doi.org/10.1182/blood.v124.21.4317.4317.

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Abstract Hematopoietic stem cells (HSCs) are capable of self-renewal and multilineage-differentiation during lifespan. HSCs are maintained in a quiescent state to avoid cellular senescence. Previous reports utilizing pharmacological inhibitors or shRNAs against p38MAPK suggest a pivotal role of p38MAPK-pRb-Ink4a signaling in induction of HSC senescence by hematological stress or chronological aging. However, no genetic evidence exists for p38MAPK-mediated cellular senescence in vivo. Here we report unexpected roles of the dominant isoform of p38MAPK family, p38α, in adult hematological system.

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25

JUNG, Sunyo, and Yong In KUK. "The Expression Level of a Specific Catalase Isozyme of Maize Mutants Alters Catalase and Superoxide Dismutase during Norflurazon-Induced Oxidative Stress in Scutella." Journal of Pesticide Science 28, no. 3 (2003): 287–92. http://dx.doi.org/10.1584/jpestics.28.287.

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26

Kato, Shinichi, Yasuyuki Ito, Fumikazu Ohkawa, and Koji Takeuchi. "W1559 Pathogenic Importance of Endothelial Isozyme of Nitric Oxide Synthase (eNOS) in Aggravation of Gastric Lesions Induced By Indomethacin and Cold-Restraint Stress in Adjuvant-Induced Arthritic Rats." Gastroenterology 136, no. 5 (2009): A—691. http://dx.doi.org/10.1016/s0016-5085(09)63184-x.

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27

Li, Qinfeng, Chao Li, Abdallah Elnwasany, et al. "PKM1 Exerts Critical Roles in Cardiac Remodeling Under Pressure Overload in the Heart." Circulation 144, no. 9 (2021): 712–27. http://dx.doi.org/10.1161/circulationaha.121.054885.

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Background: Metabolic remodeling precedes most alterations during cardiac hypertrophic growth under hemodynamic stress. The elevation of glucose utilization has been recognized as a hallmark of metabolic remodeling. However, its role in cardiac hypertrophic growth and heart failure in response to pressure overload remains to be fully illustrated. Here, we aimed to dissect the role of cardiac PKM1 (pyruvate kinase muscle isozyme 1) in glucose metabolic regulation and cardiac response under pressure overload. Methods: Cardiac-specific deletion of PKM1 was achieved by crossing the floxed PKM1 mou

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28

Laughlin, M. H., C. C. Hale, L. Novela, D. Gute, N. Hamilton, and C. D. Ianuzzo. "Biochemical characterization of exercise-trained porcine myocardium." Journal of Applied Physiology 71, no. 1 (1991): 229–35. http://dx.doi.org/10.1152/jappl.1991.71.1.229.

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The purpose of this study was to determine whether cardiac biochemical adaptations are induced by chronic exercise training (ET) of miniature swine. Female Yucatan miniature swine were trained on a treadmill or were cage confined (C) for 16–22 wk. After training, the ET pigs had increased exercise tolerance, lower heart rates during exercise at submaximal intensities, moderate cardiac hypertrophy, increased coronary blood flow capacity, and increased oxidative capacity of skeletal muscle. Myosin from both the C and ET hearts was 100% of the V3 isozyme, and there were no differences between the

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29

Sheftel, Alex D., Sangwon F. Kim, and Premysl Ponka. "Non-Heme Induction of HO-1 in Macrophages Does Not Apparently Alter Cellular Iron Levels." Blood 104, no. 11 (2004): 3693. http://dx.doi.org/10.1182/blood.v104.11.3693.3693.

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Abstract The catabolism of heme is carried out by members of the heme oxygenase (HO) family. The predominant isozyme in the digestion of erythroid heme is the inducible form, HO-1. The products of heme catabolism by HO-1 are ferrous iron, biliveridin (subsequently converted to bilirubin), and carbon monoxide (CO). In addition to its function in the recycling of erythrocyte iron, this microsomal enzyme has been implicated in a number of cytoprotective mechanisms mainly in the context of oxidant insult. HO-1 can be induced not only by heme, but also by cytokines, heat shock, metals, and other ce

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30

Bergeron, Marcelle, Donna M. Ferriero, Hendrik J. Vreman, David K. Stevenson, and Frank R. Sharp. "Hypoxia-Ischemia, but Not Hypoxia Alone, Induces the Expression of Heme Oxygenase-1 (HSP32) in Newborn Rat Brain." Journal of Cerebral Blood Flow & Metabolism 17, no. 6 (1997): 647–58. http://dx.doi.org/10.1097/00004647-199706000-00006.

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Heme oxygenase (HO) is the rate-limiting enzyme in the degradation of heme to produce bile pigments and carbon monoxide. The HO-1 isozyme is induced by a variety of agents such as heat, heme, and hydrogen peroxide. Evidence suggests that the bile pigments serve as antioxidants in cells with compromised defense mechanisms. Because hypoxia-ischemia (HI) increases the level of oxygen free radicals, the induction of HO-1 expression in the brain during ischemia could modulate the response to oxidative stress. To study the possible involvement of HO-1 in neonatal hypoxia-induced ischemic tolerance,

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31

Page, Amanda J., George Hatzinikolas, Andrew D. Vincent, Paul Cavuoto, and Gary A. Wittert. "The TRPV1 channel regulates glucose metabolism." American Journal of Physiology-Endocrinology and Metabolism 317, no. 4 (2019): E667—E676. http://dx.doi.org/10.1152/ajpendo.00102.2019.

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Endocannabinoids (ECs) mediate effects via cannabinoid receptor types 1 and 2 (CB1 and 2) and transient receptor potential channel-vanilloid subfamily member 1 (TRPV1) channels. In high-fat diet (HFD)-induced obese mice overactivity of the EC system and inhibition of CB1 increase skeletal muscle glucose uptake. We explored the role of TRPV1. Male TRPV1+/+(WT) and TRPV1−/−(KO)-mice were fed (20 wk) a standard laboratory diet (SLD) or HFD. An intraperitoneal glucose tolerance test was performed. RT-PCR was performed to measure mRNA of genes involved in glucose/lipid metabolism and the EC system

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Rahimi, Omeed, Jay Kirby, Jasmina Varagic, Brian Westwood, E. Ann Tallant, and Patricia E. Gallagher. "Angiotensin-(1–7) reduces doxorubicin-induced cardiac dysfunction in male and female Sprague-Dawley rats through antioxidant mechanisms." American Journal of Physiology-Heart and Circulatory Physiology 318, no. 4 (2020): H883—H894. http://dx.doi.org/10.1152/ajpheart.00224.2019.

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Doxorubicin (Dox) is an effective chemotherapeutic for a variety of pediatric malignancies. Unfortunately, Dox administration often results in a cumulative dose-dependent cardiotoxicity that manifests with marked oxidative stress, leading to heart failure. Adjunct therapies are needed to mitigate Dox cardiotoxicity and enhance quality of life in pediatric patients with cancer. Angiotensin-(1–7) [Ang-(1–7)] is an endogenous hormone with cardioprotective properties. This study investigated whether adjunct Ang-(1–7) attenuates cardiotoxicity resulting from exposure to Dox in male and female juven

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Wang, Peng. "The Stay-green Mutation Contributes to Enhanced Antioxidative Competence and Delays Leaf Senescence in Soybean Hybrid Z1." International Journal of Agriculture and Biology 25, no. 02 (2021): 361–72. http://dx.doi.org/10.17957/ijab/15.1676.

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The new soybean stay-green variety Jinda Zhilv No. 1 (Z1) was obtained through crossing a stay-green mutant with the super-high yielding soybean cultivar Jinda No. 74 (JD74). Here, we compared the antioxidant enzyme activities and reactive oxygen species content of the Z1 and JD74 varieties under natural and dark-induced senescence. Dark treatment was imposed at the seedling stage for 13 days. Fluorescence quantitative PCR was used to investigate the expression of isozyme genes related to superoxide dismutase (SOD), catalase (CAT) and ascorbate–glutathione cycle. The results indicated that com

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Montoya, Alvaro, Megan Wisniewski, Justin L. Goodsell, and Alexander Angerhofer. "Bidentate Substrate Binding Mode in Oxalate Decarboxylase." Molecules 29, no. 18 (2024): 4414. http://dx.doi.org/10.3390/molecules29184414.

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Oxalate decarboxylase is an Mn- and O2-dependent enzyme in the bicupin superfamily that catalyzes the redox-neutral disproportionation of the oxalate monoanion to form carbon dioxide and formate. Its best-studied isozyme is from Bacillus subtilis where it is stress-induced under low pH conditions. Current mechanistic schemes assume a monodentate binding mode of the substrate to the N-terminal active site Mn ion to make space for a presumed O2 molecule, despite the fact that oxalate generally prefers to bind bidentate to Mn. We report on X-band 13C-electron nuclear double resonance (ENDOR) expe

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Pandey, Pratima, and K. Bhanuprakash & Umesha. "Effect of Seed Priming on Biochemical Changes in Fresh and Aged Seeds of Cucumber." Journal of Agricultural Studies 5, no. 2 (2017): 62. http://dx.doi.org/10.5296/jas.v5i3.11637.

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The present investigation was conducted at the laboratory of Seed Science and Technology, Indian Institute of Horticultural Research, Hessaraghatta, and Bangalore, India during March 2014 to September 2014. The objective of the study was to identify effect of seed priming on biochemical changes in cucumber seeds. This study showed that the enhancement in seed viability and vigor in primed seeds was due to low membrane injury coupled with high enzyme activities (dehydrogenase and amylase). The priming with KH2PO4 and K2HPO4 reduced the electrical conductivity (EC) significantly in seed. With se

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Bardak, Adem, Sadettin Çelik, Oktay Erdoğan, Remzi Ekinci, and Ziya Dumlupinar. "Association Mapping of Verticillium Wilt Disease in a Worldwide Collection of Cotton (Gossypium hirsutum L.)." Plants 10, no. 2 (2021): 306. http://dx.doi.org/10.3390/plants10020306.

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Cotton (Gossypium spp.) is the best plant fiber source in the world and provides the raw material for industry. Verticillium wilt caused by Verticillium dahliae Kleb. is accepted as a major disease of cotton production. The most practical way to deal with verticillium wilt is to develop resistant/tolerant varieties after cultural practices. One of the effective selections in plant breeding is the use of marker-assisted selection (MAS) via quantitative trait loci (QTL). Therefore, in this study, we aimed to discover the genetic markers associated with the disease. Through the association mappin

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Mishima, Harutaka, Hirokazu Watanabe, Kei Uchigasaki, et al. "L-Alanine Prototrophic Suppressors Emerge from L-Alanine Auxotroph through Stress-Induced Mutagenesis in Escherichia coli." Microorganisms 9, no. 3 (2021): 472. http://dx.doi.org/10.3390/microorganisms9030472.

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In Escherichia coli, L-alanine is synthesized by three isozymes: YfbQ, YfdZ, and AvtA. When an E. coli L-alanine auxotrophic isogenic mutant lacking the three isozymes was grown on L-alanine-deficient minimal agar medium, L-alanine prototrophic mutants emerged considerably more frequently than by spontaneous mutation; the emergence frequency increased over time, and, in an L-alanine-supplemented minimal medium, correlated inversely with L-alanine concentration, indicating that the mutants were derived through stress-induced mutagenesis. Whole-genome analysis of 40 independent L-alanine prototr

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Du, Chang-Xia, Huai-Fu Fan, Shi-Rong Guo, and Takafumi Tezuka. "Applying Spermidine for Differential Responses of Antioxidant Enzymes in Cucumber Subjected to Short-term Salinity." Journal of the American Society for Horticultural Science 135, no. 1 (2010): 18–24. http://dx.doi.org/10.21273/jashs.135.1.18.

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To examine whether spermidine (SPD) modifies plant antioxidant enzyme expression in response to short-term salt stress, cucumber (Cucumis sativus) seedlings were treated with NaCl in the presence or absence of SPD for 3 days. Compared with untreated control plants, free radical production and malondialdehyde content in leaves and roots increased significantly and plant growth was suppressed under 50 mm NaCl stress. Exogenous SPD sprayed on leaves at a concentration of 1 mm alleviated salinity-mediated growth reduction. Salt stress caused a consistent increase in soluble protein content, as wel

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Tamás, L., J. Huttová, and I. Mistrík. "Effect of aluminium on peroxidase activity in roots of Al-sensitive and Al-resistant barley cultivars." Plant, Soil and Environment 48, No. 2 (2011): 76–79. http://dx.doi.org/10.17221/4363-pse.

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The effect of Al stress on peroxidase activity and peroxidase isozymes was studied in roots of two barley cultivars with contrasting sensitivity to Al. Al treatment induced a tremendous enhancement of guaiacol peroxidase activity especially in roots of Al-sensitive barley cv. Alfor. After 48 h of Al treatment activity of peroxidase in roots of cv. Alfor was up to 5.5 times higher than the control roots. In contrast, activity of peroxidase in the roots of Al-resistant cv. Bavaria was about one half than that in roots of Al-sensitive Alfor. SDS-PAGE analysis revealed that at least five peroxidas

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Priya S, Vishnu, and Somasundaram P. "INDUCED PHENOLOXIDASE PROFILES IN SILKWORM Bombyx mori (L.) UNDER BIOTIC STRESS." International Journal of Engineering Technologies and Management Research 4, no. 10 (2020): 46–52. http://dx.doi.org/10.29121/ijetmr.v4.i10.2017.105.

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Phenol oxidase (PO) is one of the stress enzyme protein in living organism. The conversion of Pro-PO into an activation form of PO required a stress protein. In the present study has emerged with the novel finding of induced phenoloxidase was identified under bacterial endotoxin viz., Lipopolysaccharide (LPS) activity using silkworm Bombyx mori as an animal model. The PO enzyme plays an important role for insect survival during pathophysiological conditions. The enzyme activity was analyzed from ten different silkworm races with two phenolic substrates viz., L-Dopa and Dopamine by Native-PAGE.

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Solórzano, Ernestina, Francisco J. Corpas, Salvador González-Gordo, and José M. Palma. "Reactive Oxygen Species (ROS) Metabolism and Nitric Oxide (NO) Content in Roots and Shoots of Rice (Oryza sativa L.) Plants under Arsenic-Induced Stress." Agronomy 10, no. 7 (2020): 1014. http://dx.doi.org/10.3390/agronomy10071014.

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Arsenic (As) is a highly toxic metalloid for all forms of life including plants. Rice is the main food source for different countries worldwide, although it can take up high amounts of As in comparison with other crops, showing toxic profiles such as decreases in plant growth and yield. The induction of oxidative stress is the main process underlying arsenic toxicity in plants, including rice, due to an alteration of the reactive oxygen species (ROS) metabolism. The aim of this work was to gain better knowledge on how the ROS metabolism and its interaction with nitric oxide (NO) operate under

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Damame, S. V., P. K. Lokhande, A. A. Kale, and S. V. Munjal. "Effect of PEG Induced Osmotic Stress on Peroxidase and Superoxide Dismutase Isozymes in Sorghum Seedlings." Vegetos- An International Journal of Plant Research 27, no. 2 (2014): 272. http://dx.doi.org/10.5958/2229-4473.2014.00044.5.

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Cong, Wei-Tao, Jin Ling, Hai-Shan Tian, et al. "Proteomic study on the protective mechanism of fibroblast growth factor 21 to ischemia–reperfusion injury." Canadian Journal of Physiology and Pharmacology 91, no. 11 (2013): 973–84. http://dx.doi.org/10.1139/cjpp-2012-0441.

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Fibroblast growth factor (FGF)-21 is a novel regulator of insulin-independent glucose transport in 3T3-L1 adipocytes and has glucose and triglyceride lowering effects in rodent models of diabetes. In this study, we found that FGF-21 can significantly attenuate ischemia–reperfusion (I/R) induced damage in H9c2 cells (rat heart). However, it is unclear which signal transduction pathway is involved in the cardioprotective effect of FGF-21. Thus, this study was designed to investigate the potential mechanism induced by FGF-21. The results showed that FGF-21 treatment prevented the oxidative stress

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Albores, Arnulfo, Christopher J. Sinal, John R. Bend, and M. George Cherian. "Selective increase of rat lung cytochrome P450 1A1 dependent monooxygenase activity after acute sodium arsenite administration." Canadian Journal of Physiology and Pharmacology 73, no. 1 (1995): 153–58. http://dx.doi.org/10.1139/y95-023.

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Arsenic is a known pulmonary, hepatic, and skin carcinogen in humans and a known inducer of stress proteins. Consequently, the ability of arsenite (As3+) to modulate isozyme-selective cytochrome P450 (P450) dependent monooxygenase activities was investigated in microsomes prepared from lung, liver, and kidney of male, adult Sprague–Dawley rats treated subcutaneously (s.c.) with sodium arsenite (75 μmol/kg body weight) 24 h before death. In the lung, the activity of P450 1A1 catalyzed 7-ethoxyresorufin O-deethylation (ERFD) was markedly (approximately 5-fold) increased in treated versus control

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Fidalgo, Fernanda, Rita Freitas, Rute Ferreira, Alberto M. Pessoa, and Jorge Teixeira. "Solanum nigrum L. antioxidant defence system isozymes are regulated transcriptionally and posttranslationally in Cd-induced stress." Environmental and Experimental Botany 72, no. 2 (2011): 312–19. http://dx.doi.org/10.1016/j.envexpbot.2011.04.007.

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Prakash Sanyal, Ravi, Amol Samant, Vishal Prashar, Hari Sharan Misra, and Ajay Saini. "Biochemical and functional characterization of OsCSD3, a novel CuZn superoxide dismutase from rice." Biochemical Journal 475, no. 19 (2018): 3105–21. http://dx.doi.org/10.1042/bcj20180516.

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Superoxide dismutases (SODs, EC 1.15.1.1) belong to an important group of antioxidant metalloenzymes. Multiple SODs exist for scavenging of reactive oxygen species (ROS) in different cellular compartments to maintain an intricate ROS balance. The present study deals with molecular and biochemical characterization of CuZn SOD encoded by LOC_Os03g11960 (referred to as OsCSD3), which is the least studied among the four rice isozymes. The OsCSD3 showed higher similarity to peroxisomal SODs in plants. The OsCSD3 transcript was up-regulated in response to salinity, drought, and oxidative stress. Ful

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Sheweita, Salah A., Amal A. Meftah, Mohamed S. Sheweita, and Mahmoud E. Balbaa. "Erectile dysfunction drugs altered the activities of antioxidant enzymes, oxidative stress and the protein expressions of some cytochrome P450 isozymes involved in the steroidogenesis of steroid hormones." PLOS ONE 15, no. 11 (2020): e0241509. http://dx.doi.org/10.1371/journal.pone.0241509.

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Objectives Infertility is a global health problem with about 15 percent of couples involved. About half of the cases of infertility are related to male-related factors. A major cause of infertility in men is oxidative stress, which refers to an imbalance between levels of reactive oxygen species (ROS) and antioxidants. Erectile dysfunction drugs (EDD), known as phosphodiesterase inhibitors (PDEIs), have been used for the treatment of ED. It has been shown that oxidative stress plays an important role in the progression of erectile dysfunction. Oxidative stress can be alleviated or decreased by

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Jung, S. "Expression level of specific isozymes of maize catalase mutants influences other antioxidants on norflurazon-induced oxidative stress." Pesticide Biochemistry and Physiology 75, no. 1-2 (2003): 9–17. http://dx.doi.org/10.1016/s0048-3575(03)00017-8.

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HERNÁNDEZ, JOSE A., LUIS A. DEL RÍO, and FRANCISCA SEVILLA. "Salt stress-induced changes in superoxide dismutase isozymes in leaves and mesophyll protoplasts fromVigna unguiculata(L.) Walp." New Phytologist 126, no. 1 (1994): 37–44. http://dx.doi.org/10.1111/j.1469-8137.1994.tb07527.x.

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Elif Kiliç, Hediye, Hatice Tunca, Tuğba Ongun Sevindik, and Ali Doğru. "Assessment of the effects of zinc on the growth and antioxidant enzymes in Scenedesmus ellipsoideus Chodat." Oceanological and Hydrobiological Studies 48, no. 3 (2019): 270–78. http://dx.doi.org/10.2478/ohs-2019-0024.

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Abstract This study explores the activity of total superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR), biomass accumulation and chlorophyll a content in Scenedesmus ellipsoideus Chodat grown under conditions of varying zinc (Zn) concentrations. In addition, the activity of different SOD isozymes (MnSOD, FeSOD and CuZnSOD) was measured separately to determine the intracellular extent of oxidative stress resulting from Zn toxicity. We found that the activity of FeSOD and MnSOD was induced by lower Zn concentration (2 μg ml−1 and 4 μg ml−1, respectively), wherea

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