Готові списки джерел за темами / Transposon mutant library screening

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Добірка наукової літератури з теми "Transposon mutant library screening"

Автор: Grafiati
Опубліковано: 3 червня 2025
Оновлено: 31 липня 2025

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Статті в журналах з теми "Transposon mutant library screening"

1

Bourgeois, Jacob, and Andrew Camilli. "High-Throughput Mutant Screening inVibrio choleraevia Transposon Sequencing." Cold Spring Harbor Protocols 2023, no. 10 (2023): pdb.prot108185. http://dx.doi.org/10.1101/pdb.prot108185.

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Анотація:
Transposon mutagenesis greatly facilitates the study of gene function in microorganisms ranging from viruses to fungi. Traditionally, one would study individual transposon mutants with interesting phenotypes one mutant at a time. Here, we describe methods for the study of tens of thousands of transposon mutants in parallel in the bacterial pathogenVibrio choleraeusing transposon-sequencing. The first section outlines methods for making a saturated transposon mutant library. The second section outlines methods for massively parallel sequencing of the transposon junctions. The third section outl
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2

Bourgeois, Jacob, and Andrew Camilli. "High-Throughput Mutant Screening via Transposon Sequencing." Cold Spring Harbor Protocols 2023, no. 10 (2023): pdb.top107867. http://dx.doi.org/10.1101/pdb.top107867.

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Transposon mutagenesis has been the method of choice for genetic screens and selections in bacteria by virtue of the transposon being linked to the disrupted gene, simplifying its identification. Transposon sequencing (Tn-seq) is a high-throughput version of transposon mutant screening, in which massively parallel sequencing is used to simultaneously follow the fitness of all mutants in a complex library. In a single experiment, one can use Tn-seq to interrogate the contribution of all genes of a bacterium to fitness under a condition of interest. Here, we introduce a method to construct a sat
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3

Hudson, P., T. S. Gorton, L. Papazisi, K. Cecchini, S. Frasca, and S. J. Geary. "Identification of a Virulence-Associated Determinant, Dihydrolipoamide Dehydrogenase (lpd), in Mycoplasma gallisepticum through In Vivo Screening of Transposon Mutants." Infection and Immunity 74, no. 2 (2006): 931–39. http://dx.doi.org/10.1128/iai.74.2.931-939.2006.

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ABSTRACT To effectively analyze Mycoplasma gallisepticum for virulence-associated determinants, the ability to create stable genetic mutations is essential. Global M. gallisepticum mutagenesis is currently limited to the use of transposons. Using the gram-positive transposon Tn4001mod, a mutant library of 110 transformants was constructed and all insertion sites were mapped. To identify transposon insertion points, a unique primer directed outward from the end of Tn4001mod was used to sequence flanking genomic regions. By comparing sequences obtained in this manner to the annotated M. gallisep
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4

Held, Kiara, Elizabeth Ramage, Michael Jacobs, Larry Gallagher, and Colin Manoil. "Sequence-Verified Two-Allele Transposon Mutant Library for Pseudomonas aeruginosa PAO1." Journal of Bacteriology 194, no. 23 (2012): 6387–89. http://dx.doi.org/10.1128/jb.01479-12.

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ABSTRACTMutant hunts using comprehensive sequence-defined libraries make it possible to identify virtually all of the nonessential functions required for different bacterial processes. However, the success of such screening depends on the accuracy of mutant identification in the mutant library used. To provide a high-quality library forPseudomonas aeruginosaPAO1, we created a sequence-verified collection of 9,437 transposon mutants that provides genome coverage and includes two mutants for most genes. Mutants were cherry-picked from a larger library, colony-purified, and resequenced both indiv
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5

Chen, Qiang, Hui Wu, and Paula M. Fives-Taylor. "Construction of a Novel Transposon Mutagenesis System Useful in the Isolation of Streptococcus parasanguis Mutants Defective in Fap1 Glycosylation." Infection and Immunity 70, no. 12 (2002): 6534–40. http://dx.doi.org/10.1128/iai.70.12.6534-6540.2002.

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ABSTRACT Streptococcus parasanguis, a primary colonizer of the tooth surface, has long, peritrichous fimbriae. A fimbria-associated protein, Fap1, is identified as an adhesin of S. parasanguis FW213. The mature Fap1 protein is glycosylated, and the glycosylation is required for fimbria biogenesis and bacterial adhesion. Little is known about the mechanism of Fap1 glycosylation due to the lack of identifiable mutants. A novel transposon mutagenesis system was established and used to generate a mutant library. Screening of the library with a monoclonal antibody specific for a glycan epitope of F
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6

Wiegand, Irith, Alexandra K. Marr, Elena B. M. Breidenstein, Kristen N. Schurek, Patrick Taylor, and Robert E. W. Hancock. "Mutator Genes Giving Rise to Decreased Antibiotic Susceptibility in Pseudomonas aeruginosa." Antimicrobial Agents and Chemotherapy 52, no. 10 (2008): 3810–13. http://dx.doi.org/10.1128/aac.00233-08.

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ABSTRACT Screening of the PA14 genomic transposon mutant library for resistance to ceftazidime, tobramycin, and ciprofloxacin led to the discovery of several mutants that appeared in more than one screen. Testing of the frequency of mutation to ciprofloxacin resistance revealed previously known mutator genes, including mutS and mutL, as well as mutators that have not yet been described for P. aeruginosa, including PA3958 and RadA (PA4609).
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7

Ho, Peiying, Linh Chi Dam, Wei Ren Ryanna Koh, et al. "Screening of the PA14NR Transposon Mutant Library Identifies Genes Involved in Resistance to Bacteriophage Infection in Pseudomomas aeruginosa." International Journal of Molecular Sciences 25, no. 13 (2024): 7009. http://dx.doi.org/10.3390/ijms25137009.

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Анотація:
Multidrug-resistant P. aeruginosa infections pose a serious public health threat due to the rise in antimicrobial resistance. Phage therapy has emerged as a promising alternative. However, P. aeruginosa has evolved various mechanisms to thwart phage attacks, making it crucial to decipher these resistance mechanisms to develop effective therapeutic strategies. In this study, we conducted a forward-genetic screen of the P. aeruginosa PA14 non-redundant transposon library (PA14NR) to identify dominant-negative mutants displaying phage-resistant phenotypes. Our screening process revealed 78 mutant
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Ghatage, Abhay, Rachna Pandey, and G.R Pathade. "Transposon Mutagenesis for enhancement of nitrogen fixing ability of Rhizobium japonicum infecting Vigna radiata." Ecology, Environment and Conservation 29 (2023): 153–56. http://dx.doi.org/10.53550/eec.2023.v29i03s.030.

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This study developed mutant library of 800 mutants with transposition frequency of 3.7 x 10- 6. Transposon mutagenesis is an easy and highly effective method for generating bacteria with improved characteristics and gene knockouts. The slow growing Rhizobium japonicum isolated from root nodules of locally grown mung bean plant. The isolated strain was subjected for transposon mutagenesis to produce 800 mutants. These 800 mutants were screened for nodulation. After screening of these 800 mutants, the100 mutants showed pink coloured nodulation to mung bean plant. Out of 100 mutants 10 were promi
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9

Karsi, Attila, Nagihan Gülsoy, Erin Corb, Pradeep R. Dumpala, and Mark L. Lawrence. "High-Throughput Bioluminescence-Based Mutant Screening Strategy for Identification of Bacterial Virulence Genes." Applied and Environmental Microbiology 75, no. 7 (2009): 2166–75. http://dx.doi.org/10.1128/aem.02449-08.

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ABSTRACT A high-throughput bioluminescence screening procedure for identification of virulence genes in bacteria was developed and applied to the fish pathogen Edwardsiella ictaluri. A random transposon mutant library expressing bioluminescence was constructed and robotically arrayed on 384-well plates. Mutants were cultivated and mixed with catfish serum and neutrophils in 96-well plates, and bioluminescence was used to detect mutants that are more susceptible to killing by these host factors. The virulence and vaccine efficacy of selected mutants were determined in channel catfish. Transposo
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10

Maroncle, Nathalie, Damien Balestrino, Chantal Rich, and Christiane Forestier. "Identification of Klebsiella pneumoniae Genes Involved in Intestinal Colonization and Adhesion Using Signature-Tagged Mutagenesis." Infection and Immunity 70, no. 8 (2002): 4729–34. http://dx.doi.org/10.1128/iai.70.8.4729-4734.2002.

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ABSTRACT Klebsiella pneumoniae is an opportunistic pathogen responsible for nosocomial infections that initially colonize the intestinal tract of patients. Signature-tagged mutagenesis was used to identify genes required for this function. A library of 2,200 mutants was analyzed for the inability of the mutants to survive in a murine model of intestinal colonization and to adhere to human intestinal cells (Int-407) in vitro. Twenty-nine attenuated mutants were selected for further analyses after competition assays against the wild-type strain. Whatever the screening model, most of the transpos
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Дисертації з теми "Transposon mutant library screening"

1

Thaipisuttikul, Lyarit. "Identification of genes required for anaerobic growth of Pseudomonas aeruginosa using a comprehensive transposon mutant library /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/10281.

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2

Kean, Iain. "Development of polymerase lithographic technology and analysis of an E. coli transposon mutant library for nitrite sensitivity." Thesis, University of Sheffield, 2015. http://etheses.whiterose.ac.uk/8169/.

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3

Li, Yanhui. "Construction and Analysis of a Genome-Wide Insertion Library in Schizosaccharomyces pombe Reveals Novel Aspects of DNA Repair." Case Western Reserve University School of Graduate Studies / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1413927620.

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4

Flor, Duro Alejandra. "Characterization of Genes and Functions Required by Multidrug-resistant Enterococci to Colonize the Intestine." Doctoral thesis, Universitat Politècnica de València, 2021. http://hdl.handle.net/10251/166494.

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[ES] Las bacterias resistentes a múltiples antibióticos, como el Enterococo resistente a vancomicina (ERV), son un problema creciente en los pacientes hospitalizados, por lo que se necesita estrategias alternativas para combatir estos patógenos. Las infecciones causadas por ERV suelen comenzar con la colonización del tracto intestinal, un paso crucial que se afectado por la presencia de la microbiota. Sin embargo, los antibióticos alteran la microbiota y esto promueve la colonización de ERV. Una vez que el patógeno ha colonizado el intestino, alcanza niveles muy altos pudiendo diseminar a otro
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5

Chen, Yi-Yin, and 陳依吟. "Mycobacterium marinum mmar_2318 and mmar_2319 are Responsible for Lipooligosaccharide Biosynthesis and Virulence towards Dictyostelium: screening in a transposon mutant library." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/15798083985838293381.

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博士<br>國立臺灣大學<br>微生物學研究所<br>104<br>Resistance to phagocyte killing is an important virulence factor in mycobacteria. Dictyostelium has been used to study the interaction between phagocytes and bacteria, given its similarity to the mammalian macrophage. Here, we investigated the genes responsible for virulence to Dictyostelium by screening 1728 transposon mutants of the Mycobacterium marinum NTUH-M6094 strain. A total of 30 mutants that were permissive for Dictyostelium growth were identified. These mutants revealed interruptions in 20 distinct loci. Of the 20 loci, six genes (losA, mmar_2318, m
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6

Syu, Zun-Jie, and 許尊傑. "Identification of rice genes associated with susceptibility or resistance to Pythium through the screening of Taiwan Rice Insertional Mutant library." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/45697639703421883766.

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碩士<br>國立中興大學<br>植物病理學系所<br>102<br>Chapter 1 Rice is one of the most important staple foods that feeds more than three billion people in the world. Rice diseases occurred during rice growing in the fields, such as seedling blight, blast, sheath blight, bacterial blight and bakana disease, and are a major obstacle for achieving optimal yields. Seedling blight is also known as water-mold disease, seed rot, and seedling damping-off. The disease is caused by several different pathogens and Pythium species is one of the major pathogens. Pythium infects roots of rice by zoospores or oospores spread i
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7

Shee, Somnath. "Manipulating Bacterial and Host Reactive Oxygen Species (ROS)- based mechanisms to potentiate killing of Mycobacterium tuberculosis (Mtb)." Thesis, 2021. https://etd.iisc.ac.in/handle/2005/5680.

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Mycobacterium tuberculosis (Mtb) is evolutionarily equipped to resist exogenous reactive oxygen species but shows vulnerability to an increase in endogenous ROS (eROS). Since eROS is an unavoidable consequence of aerobic metabolism, understanding how eROS levels are controlled is essential yet remains uncharacterized. By combining the Mrx1-roGFP2 redox biosensor with transposon mutagenesis, we identified 368 genes (redoxosome) responsible for maintaining non-toxic levels of eROS in Mtb. Integrating redoxosome with a global network of protein-protein interactions and transcriptional regulators
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Частини книг з теми "Transposon mutant library screening"

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Endres, Jennifer L., Vijaya Kumar Yajjala, Paul D. Fey, and Kenneth W. Bayles. "Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus." In Microbial Transposon Mutagenesis. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9570-7_3.

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2

Maliszewski, Katherine L. "Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/7651_2015_281.

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3

Widhelm, Todd J., Vijay Kumar Yajjala, Jennifer L. Endres, Paul D. Fey, and Kenneth W. Bayles. "Methods to Generate a Sequence-Defined Transposon Mutant Library in Staphylococcus epidermidis Strain 1457." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-736-5_12.

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4

Yajjala, Vijaya Kumar, Todd J. Widhelm, Jennifer L. Endres, Paul D. Fey, and Kenneth W. Bayles. "Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using bursa aurealis." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/7651_2014_189.

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5

Jankowicz-Cieslak, Joanna, Ivan L. Ingelbrecht, and Bradley J. Till. "Mutation Detection in Gamma-Irradiated Banana Using Low Coverage Copy Number Variation." In Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana. Springer Berlin Heidelberg, 2022. http://dx.doi.org/10.1007/978-3-662-64915-2_8.

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AbstractMutagenesis of in vitro propagated bananas is an efficient method to introduce novel alleles and broaden genetic diversity. The FAO/IAEA Plant Breeding and Genetics Laboratory previously established efficient methods for mutation induction of in vitro shoot tips in banana using physical and chemical mutagens as well as methods for the efficient discovery of ethyl methanesulphonate (EMS) induced single nucleotide mutations in targeted genes. Officially released mutant banana varieties have been created using gamma rays, a mutagen that can produce large genomic changes such as insertions
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6

Dai, Junbiao, and Jef D. Boeke. "Strain Construction and Screening Methods for a Yeast Histone H3/H4 Mutant Library." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-477-3_1.

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Johnson, Jeremiah G., and Victor J. DiRita. "Generation and Screening of an Insertion Sequencing-Compatible Mutant Library of Campylobacter jejuni." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6536-6_21.

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8

Yin, Dezhong, and Yinduo Ji. "Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library." In Microbial Gene Essentiality: Protocols and Bioinformatics. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-321-9_19.

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9

Torres, Marta, Almudena Gonzalez-Mula, Delphine Naquin, and Denis Faure. "Construction of a Transposon Mutant Library in the Pathogen Agrobacterium tumefaciens C58 and Identification of Genes Involved in Gall Niche Exploitation and Colonization." In Microbial Environmental Genomics (MEG). Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2871-3_11.

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10

Nithya, N., and K. Anbarasu. "Computational screening of the lead compounds for obesity by targeting human FTO mutant E234P with various ligand library using in-silico approach." In Applications of Mathematics in Science and Technology. CRC Press, 2025. https://doi.org/10.1201/9781003606659-183.

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Тези доповідей конференцій з теми "Transposon mutant library screening"

1

Wong, Yee-Chin, Arnab Pain, and Sheila Nathan. "Construction of a large-scale Burkholderia cenocepacia J2315 transposon mutant library." In THE 2014 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2014 Postgraduate Colloquium. AIP Publishing LLC, 2014. http://dx.doi.org/10.1063/1.4895267.

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Kennedy, Perry C., Marc C. Guilanotti, Travis LsVoi, and Said M. Sebti. "Abstract 2580: Screening of a mixture-based synthetic combinatorial library identifies small molecules that inhibit the ability of GTP to displace mant-GDP from mutant G12D KRas." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-2580.

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Звіти організацій з теми "Transposon mutant library screening"

1

Horwitz, Benjamin, and Nicole M. Donofrio. Identifying unique and overlapping roles of reactive oxygen species in rice blast and Southern corn leaf blight. United States Department of Agriculture, 2017. http://dx.doi.org/10.32747/2017.7604290.bard.

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Анотація:
Plants and their fungal pathogens both produce reactive oxygen species (ROS). CytotoxicROS act both as stressors and signals in the plant-fungal interaction. In biotrophs, a compatible interaction generates little ROS, but is followed by disease. An incompatible interaction results in a strong oxidative burst by the host, limiting infection. Necrotrophs, in contrast, thrive on dead and dying cells in an oxidant-rich local environment. Rice blast, Magnaportheoryzae, a hemibiotroph, occurs worldwide on rice and related hosts and can decimate enough rice each year to feed sixty million people. Co
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Sela, Shlomo, and Michael McClelland. Desiccation Tolerance in Salmonella and its Implications. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7594389.bard.

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Salmonella enterica is a worldwide food-borne pathogen, which regularly causes large outbreaks of food poisoning. Recent outbreaks linked to consumption of contaminated foods with low water-activity, have raised interest in understanding the factors that control fitness of this pathogen to dry environment. Consequently, the general objective of this study was to extend our knowledge on desiccation tolerance and long-term persistence of Salmonella. We discovered that dehydrated STm entered into a viable-but-nonculturable state, and that addition of chloramphenicol reduced bacterial survival. Th
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Shpigel, Nahum, Raul Barletta, Ilan Rosenshine, and Marcelo Chaffer. Identification and characterization of Mycobacterium paratuberculosis virulence genes expressed in vivo by negative selection. United States Department of Agriculture, 2004. http://dx.doi.org/10.32747/2004.7696510.bard.

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Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of a severe inflammatory bowel disease (IBD) in ruminants, known as Johne’s disease or paratuberculosis. Johne’s disease is considered to be one of the most serious diseases affecting dairy cattle both in Israel and worldwide. Heavy economic losses are incurred by dairy farmers due to the severe effect of subclinical infection on milk production, fertility, lower disease resistance and early culling. Its influence in the United States alone is staggering, causing an estimated loss of $1.5 billion to the agriculture indu
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Li, Li, Joseph Burger, Nurit Katzir, Yaakov Tadmor, Ari Schaffer, and Zhangjun Fei. Characterization of the Or regulatory network in melon for carotenoid biofortification in food crops. United States Department of Agriculture, 2015. http://dx.doi.org/10.32747/2015.7594408.bard.

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The general goals of the BARD research grant US-4423-11 are to understand how Or regulates carotenoid accumulation and to reveal novel strategies for breeding agricultural crops with enhanced β-carotene level. The original objectives are: 1) to identify the genes and proteins in the Or regulatory network in melon; 2) to genetically and molecularly characterize the candidate genes; and 3) to define genetic and functional allelic variation of these genes in a representative germplasm collection of the C. melo species. Or was found by the US group to causes provitamin A accumulation in chromoplas
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5

Shpigel, Nahum Y., Ynte Schukken, and Ilan Rosenshine. Identification of genes involved in virulence of Escherichia coli mastitis by signature tagged mutagenesis. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7699853.bard.

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Mastitis, an inflammatory response of the mammary tissue to invading pathogenic bacteria, is the largest health problem in the dairy industry and is responsible for multibillion dollar economic losses. E. coli are a leading cause of acute mastitis in dairy animals worldwide and certainly in Israel and North America. The species E. coli comprises a highly heterogeneous group of pathogens, some of which are commensal residents of the gut, infecting the mammary gland after contamination of the teat skin from the environment. As compared to other gut microflora, mammary pathogenic E. coli (MPEC) m
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