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Статті в журналах з теми "Virus detection method"

Автор: Grafiati

Опубліковано: 28 травня 2022

Оновлено: 31 липня 2025

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1

Liu, Yanbing, Shousheng Jia, and Congcong Xing. "A Novel Behavior-Based Virus Detection Method for Smart Mobile Terminals." Discrete Dynamics in Nature and Society 2012 (2012): 1–12. http://dx.doi.org/10.1155/2012/262193.

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Анотація:

The security of smart mobile terminals has been an increasingly important issue in recent years. While there are extensive researches on virus detections for smart mobile terminals, most of them share the same framework of virus detection as that for personal computers, and few of them tackle the problem from the standpoint of detection methodology. In this paper, we propose a behavior-based virus detection method for smart mobile terminals which signals the existence of malicious code through identifying the anomaly of user behaviors. We first propose a model to collect and analyze user behav

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2

Abdellatif, Berkat. "Metamorphic computer virus detection by Case-Based Reasoning (CBR) methods." International Journal of Software Engineering & Applications (IJSEA) 2, no. 4 (2021): 1–10. https://doi.org/10.5281/zenodo.4730062.

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Metamorphic virus employs code obfuscation techniques to mutate itself. It absconds from signature based detection system by modifying internal structure without compromising original functionality. -In this paper, we propose a new method, for detecting metamorphic computer viruses, that is based on the technique of Case-Based Reasoning (CBR). In this method: -Can detect similar viruses with high probability. - The updating of the virus database is done automatically without connecting to the Internet. Whenever a new virus is detected, it will be automatically added to the database used by our

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3

САВЕНКО, БОГДАН. "WORM-VIRUS DETECTION METHOD ACCORDING TO MULTI-CLASS CLASSIFICATION." Herald of Khmelnytskyi National University. Technical sciences 331, no. 1 (2024): 18–28. http://dx.doi.org/10.31891/2307-5732-2024-331-2.

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The work presents the results of research on worm viruses and methods of their detection. Malware distribution happens all the time. The analyzed modern tools and systems for prevention, detection and countermeasures against malicious software and computer attacks are quite effective, provide a high percentage of detection and function at an adequate level. But criminals constantly study the capabilities of such tools and systems, improve malicious software and computer attacks, and achieve certain results. Therefore, developers of tools and systems for prevention, detection and countermeasure

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4

Tyumentseva, M. A., A. I. Tyumentsev, A. N. Prelovskaya, and V. G. Akimkin. "Optimization of a Method for Detecting Single copies of Hepatitis B Virus DNA using CRISPR/Cas systems." Epidemiology and Vaccinal Prevention 23, no. 6 (2025): 114–28. https://doi.org/10.31631/2073-3046-2024-23-6-114-128.

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Relevance. Hepatitis B virus (HBV) is the etiologic agent of acute and chronic hepatitis B in humans. WHO recommends the use of sensitive laboratory assays based on nucleic acid amplification methods to detect HBV DNA. A method for detecting single copies of hepatitis B virus DNA using CRISPR/Cas systems was previously developed for ultrasensitive detection of HBV DNA.Aims. The aim of present study was to optimize the method for detecting single copies of hepatitis B virus DNA using CRISPR/Cas systems.Materials and methods. To obtain amplified fragments of the hepatitis B virus genome, 22 olig

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5

Lee, Brian W., Russell F. Bey, Mary J. Baarsch, and Randy R. Simonson. "ELISA Method for Detection of Influenza A Infection in Swine." Journal of Veterinary Diagnostic Investigation 5, no. 4 (1993): 510–15. http://dx.doi.org/10.1177/104063879300500402.

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An antigen-capture enzyme-linked immunosorbent assay (ELISA) was developed to monitor virus shedding associated with experimental infection with a field strain of swine influenza in pigs. The assay consisted of a monoclonal anti-nucleoprotein capture antibody and a biotinylated rabbit anti-influenza A (H1N1) sandwich antibody. The antigen-capture system was capable of detecting as little as 1 ng/ml purified virus. The ELISA system surpassed egg cultivation procedures in the detection of low levels of shedding virus. Egg cultivation procedures indicated that most viral shedding had ceased by da

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6

Komínek, P. "Selection of RNA isolation method for molecular detection of grapevine viruses." Plant Protection Science 38, SI 2 - 6th Conf EFPP 2002 (2017): 267–70. http://dx.doi.org/10.17221/10463-pps.

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Grapevines infected with Grapevine leafroll-associated virus-1 (GLRaV-1) and Grapevine leafroll-associated virus-3 (GLRaV-3) were selected. Total RNA was isolated from grapevine phloem tissue scrapped from dormant canes by three different methods: extraction with urea buffer followed with phenol-chloroform extraction, method using Concert<sup>TM</sup> reagent (Invitrogen) followed with chloroform-isopropylalcohol extraction, and procedure using RNeasy Plant Mini Kit (Qiagen). The highest yield of RNA was obtained using Concert<sup>TM</sup> reagent. If this RNA was used

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7

Ilyas, Syafruddin. "Human Papillomavirus: Detection Method and Infection." International Journal of Ecophysiology 4, no. 1 (2023): 82–91. http://dx.doi.org/10.32734/ijoep.v4i1.11153.

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The Human Papilloma Virus (HPV) is a small non-enveloped DNA virus, around 8000 bp in size and only humans become its host by infecting skin epithelial tissue, human oral mucosa and anogenital epithelium. Human Papillomavirus is often found in patients and is ranked as the second most malignant disease in women, belonging to the Alphapapillomavirus genus. HPV infection can be identified through the structure of the HPV virus itself and the particles contained therein which initiate the carcinogenic process of its host. The research methods used in this study are literature studies. The literat

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8

Rowhani, Adib, Lenka Biardi, Geoffrey Routh, Steve D. Daubert, and Deborah A. Golino. "Development of a Sensitive Colorimetric-PCR Assay for Detection of Viruses in Woody Plants." Plant Disease 82, no. 8 (1998): 880–84. http://dx.doi.org/10.1094/pdis.1998.82.8.880.

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Diagnostic methods employing the polymerase chain reaction (PCR) provide the most sensitive means currently available for detecting viruses in woody plants. A new technique has been tested that does not rely on gel electrophoresis or molecular hybridization to detect virus-specific PCR products. This colorimetric method for detection of PCR products from woody plants was demonstrated to be at least as sensitive as gel analysis. When combined with immunocapture of virions from plant sap, colorimetric detection provides a means to apply PCR technology to a large number of samples. Here, we repor

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9

Moeis, Maelita Ramdani, Anis Puji Rahayu, Nisa Ihsani, and Wulan Pertiwi. "Coronavirus-SARS-CoV-2: Biology and Problems in rRT-PCR Detection." Borneo Journal of Pharmacy 3, Special-1 (2020): 136–45. http://dx.doi.org/10.33084/bjop.v3ispecial-1.1429.

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Coronavirus disease 2019 (COVID-19) first appeared in China in December 2019 and was declared a pandemic by the World Health Organization. COVID-19 is caused by Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2), a new virus previously unknown to humans. Here we look at what is known about this virus, the main method for detecting the presence of this virus in a person who is used as a golden standard, and the problems that could arise in this detection method. Understanding the biology of the virus and the strengths and weaknesses of the detection method are important for patient m

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10

Cheremiskina, A. A., D. V. Shanshin, V. M. Generalov, et al. "Method for rapid detection of recombinant protein E of West Nile virus." Izmeritel`naya Tekhnika, no. 10 (December 9, 2024): 57–64. https://doi.org/10.32446/0368-1025it.2024-10-57-64.

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Анотація:

It was noted that the detection time of West Nile virus protein E by standard methods – immunoenzyme assay for West Nile virus antigen, antibody seroconversion, polymerase chain reaction with reverse transcription, virus isolation and neutralization assay, is at least one hour. West Nile virus (genus Flavivirus) belongs to the Japanese encephalitis antigenic complex of the family Flaviviridae and is capable of causing West Nile fever or severe West Nile disease. To increase the detection rate of recombinant protein E of West Nile virus, an express detection method using the developed promising

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11

Thür, B., M. Hilbe, M. Strasser, and F. Ehrensperger. "Immunohistochemical diagnosis of pestivirus infection associated with bovine and ovine abortion and perinatal death." American Journal of Veterinary Research 58, no. 12 (1997): 1371–75. http://dx.doi.org/10.2460/ajvr.1997.58.12.1371.

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SUMMARY Objective To establish a reliable, rapid, economical method for detection of pestivirus infection in bovine and ovine fetuses and to examine participation of these viruses in abortions and neonatal mortality. Animals 213 bovine and 31 ovine fetuses, as well as 36 newborn calves and 25 lambs, which had died within 3 days after birth, were tested for bovine viral diarrhea virus (BVDV) and border disease virus by use of different methods. Procedure Detection of BVDV in fetuses was performed by immunohistochemical methods, using a panel of monoclonal antibodies against pestivirus antigens

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12

Miao, Chun Yu, and Li Na Chen. "A Research of Virus Detection Combined Dynamic and Static Analysis Methods." Advanced Materials Research 187 (February 2011): 625–30. http://dx.doi.org/10.4028/www.scientific.net/amr.187.625.

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we present a virus detection system based on the D-S theory of evidence, in which the dynamic and static analysis methods are combined. The detection engine applies two types of classifier, support vector amchine and probabilistic neural network to detect the virus. For SVM classifier, we extract the feature vector by monitoring the samples. And the static feature of samples is used in the probabilistic neural network classifier. Finally, the D-S theory of evidence is used to combine the contribution of each individual classifier to give the final decision.experiments show the presented method

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13

Sui, Xin, Xu Zhang, Dongliang Fei, Zhen Zhang, and Mingxiao Ma. "Simultaneous rapid detection of Hantaan virus and Seoul virus using RT-LAMP in rats." PeerJ 6 (January 8, 2019): e6068. http://dx.doi.org/10.7717/peerj.6068.

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Background Hemorrhagic fever with renal syndrome is in most cases caused by the Hantaan virus (HTNV) and Seoul virus (SEOV). To develop and apply reverse transcription loop-mediated isothermal amplification (RT-LAMP) to detect HTNV and SEOV simultaneously, which was faster, more cost effective, and easier to perform as the target gene amplified rapidly. In this article an assay based on LAMP is demonstrated, which only employs such apparatus as a water bath or a heat block. Methods A chromogenic method using the calcein/Mn2+ complex and real-time turbidity monitoring method were used to assess

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14

REBELO-de-ANDRADE, H., and M. C. ZAMBON. "Different diagnostic methods for detection of influenza epidemics." Epidemiology and Infection 124, no. 3 (2000): 515–22. http://dx.doi.org/10.1017/s0950268899003751.

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Linking continuous community-based morbidity recording of influenza-like illness (ILI) with virological sampling has consistently proved its value as one of the earliest indicators of circulating influenza activity. The clinical morbidity recording in the Portuguese national surveillance network, during a 7-year period, and the contribution of different diagnostic techniques, including virus isolation, multiplex RT–PCR, immunocapture enzyme linked immunoassay (EIA) and complement fixation tests (CFTs) for the detection of influenza in such a community-based setting is described and evaluated i

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15

Zou, Mengsong, Lansheng Han, Ming Liu, and Qiwen Liu. "Virus Detection Method based on Behavior Resource Tree." Journal of Information Processing Systems 7, no. 1 (2011): 173–86. http://dx.doi.org/10.3745/jips.2011.7.1.173.

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16

Maldonado, J., L. Valls, and P. Riera. "Method for rapid detection of swine influenza virus." Veterinary Record 165, no. 11 (2009): 328. http://dx.doi.org/10.1136/vr.165.11.328.

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17

Grizzle, JM, I. Altinok, and AD Noyes. "PCR method for detection of largemouth bass virus." Diseases of Aquatic Organisms 54 (2003): 29–33. http://dx.doi.org/10.3354/dao054029.

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18

Wang, Qian, Shiwen Wu, Jiangbing Shuai, et al. "Dual Gene Detection of H5N1 Avian Influenza Virus Based on Dual RT-RPA." Molecules 29, no. 12 (2024): 2801. http://dx.doi.org/10.3390/molecules29122801.

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The H5N1 avian influenza virus seriously affects the health of poultry and humans. Once infected, the mortality rate is very high. Therefore, accurate and timely detection of the H5N1 avian influenza virus is beneficial for controlling its spread. This article establishes a dual gene detection method based on dual RPA for simultaneously detecting the HA and M2 genes of H5N1 avian influenza virus, for the detection of H5N1 avian influenza virus. Design specific primers for the conserved regions of the HA and M2 genes. The sensitivity of the dual RT-RPA detection method for HA and M2 genes is 1

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19

Weng, Xiaoxing, Chen Li, Changqing Chen, Gang Wang, Chenghao Xia, and Lianyou Zheng. "A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy." Micromachines 14, no. 6 (2023): 1118. http://dx.doi.org/10.3390/mi14061118.

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Aiming at the problem of how to achieve the rapid detection of pathogenic microorganisms, this paper takes tobacco ringspot virus as the detection object, designs the impedance detection and analysis platform of tobacco ringspot virus based on microfluidic impedance method, establishes an equivalent circuit model to analyze the experimental results, and determines the optimal detection frequency of tobacco ringspot virus detection. Based on this frequency, an impedance–concentration regression model was established for the detection of tobacco ringspot virus in a tobacco ringspot virus detecti

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20

Zhang, Hao, Yuan Yao, Zhi Chen, et al. "Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method." Biosensors 12, no. 4 (2022): 213. http://dx.doi.org/10.3390/bios12040213.

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African swine fever (ASF) is a swine disease with a very high fatality rate caused by a complex double-stranded DNA virus. The fluorescence PCR detection method is widely used for virus nucleic acid detection. Surface plasmon resonance (SPR) is a label-free and real-time detection method, unlike the fluorescence PCR detection method. In this research, we detected the loop-mediated isothermal amplification (LAMP) products of the African swine fever virus by using the SPR and fluorescence methods separately and simultaneously. By comparing the positive and negative control results, we found that

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21

Han, Sheng, Tingting Zhou, Fengqin Zhang, Jing Feng, Chenggui Han, and Yushanjiang Maimaiti. "One-Step Multiplex RT-PCR Method for Detection of Melon Viruses." Microorganisms 12, no. 11 (2024): 2337. http://dx.doi.org/10.3390/microorganisms12112337.

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This study presents a one-step multiplex reverse transcription polymerase chain reaction (RT-PCR) method for the simultaneous detection of multiple viruses affecting melon crops. Viruses such as Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Squash mosaic virus (SqMV), Tobacco mosaic virus (TMV), Papaya ring spot virus (PRSV), and Melon yellow spot virus (MYSV) pose a great threat to melons. The mixed infection of these viruses is the most common observation in the melon-growing fields. In this study, we surveyed northern Xingjiang (Altay, Chan

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22

Zemlyanaya, D., N. Boldyrikhin, A. Svizhenko, and B. Yukhnov. "Virus signature detection algorithm." Journal of Physics: Conference Series 2131, no. 2 (2021): 022086. http://dx.doi.org/10.1088/1742-6596/2131/2/022086.

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Abstract The purpose of this research is to develop an algorithm for finding virus signatures. The method of searching for virus signatures was analyzed to achieve this goal. A brief description of the Boyer-Moore algorithm was also considered. The result of the research is a new algorithm that optimizes the speed of finding virus signatures by scanning the beginning and end of the file, since these are common cases where viruses are located. The practical significance of this research lies in the development of an algorithm for finding virus signatures, which reduces the risk of infection of

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23

Guslianto, Safrida Ika, Khairunnas Khairunnas, Tachiyya Nailal Khusna, and Miftahul Jannah. "Real Time Mask Detection Using Viola Jones Method." PIKSEL : Penelitian Ilmu Komputer Sistem Embedded and Logic 12, no. 1 (2024): 119–26. http://dx.doi.org/10.33558/piksel.v12i1.9028.

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The coronavirus disease (COVID-19) pandemic requires us to wear masks when doing direct contact with other people. The use of masks is mandatory in some places to break the chain of the spread of the COVID-19 virus. The spread of this virus occurs through the respiratory tract. Wearing masks is a form of our efforts to suppress the spread of this virus. This study detects human face objects in a room to determine faces that use masks and faces without using masks. The research was conducted using the Viola Jones Method in the OpenCV-Python library. This paper resulted in a good accuracy of the

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24

Donaldson, Kim A., Marianne F. Kramer, and Daniel V. Lim. "A rapid detection method for Vaccinia virus, the surrogate for smallpox virus." Biosensors and Bioelectronics 20, no. 2 (2004): 322–27. http://dx.doi.org/10.1016/j.bios.2004.01.029.

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25

Gospodinović, Hristina, Edita Grego, Ljiljana Pavlović, et al. "HPV virus genotyping by RT-PCR method." Glasnik javnog zdravlja 96, no. 3 (2022): 342–50. http://dx.doi.org/10.5937/serbjph2203342g.

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Cervical cancer is the second most common type of cancer of the female reproductive organs i.e., the third most common malignant tumor among women globally. The significance of HPV genotyping, a method used to identify specific HPV genotypes, has been recognized in recent decades as an important tool for the early detection of cervical cancer risk. In recent years, great progress has been made in understanding HPV molecular biology, a large number of tests have been developed, and there is ongoing research on the association between their diagnostic and therapeutic use. In the conducted resear

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26

Yuan, Xiangfen, Jizhou Lv, Xiangmei Lin, et al. "Multiplex detection of six swine viruses on an integrated centrifugal disk using loop-mediated isothermal amplification." Journal of Veterinary Diagnostic Investigation 31, no. 3 (2019): 415–25. http://dx.doi.org/10.1177/1040638719841096.

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Advances in molecular testing and microfluidic technologies have opened new avenues for rapid detection of animal viruses. We used a centrifugal microfluidic disk (CMFD) to detect 6 important swine viruses, including foot-and-mouth disease virus, classical swine fever virus, porcine reproductive and respiratory swine virus–North American genotype, porcine circovirus 2, pseudorabies virus, and porcine parvovirus. Through integrating the loop-mediated isothermal amplification (LAMP) method and microfluidic chip technology, the CMFD could be successfully performed at 62℃ in 60 min. The detection

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27

Liu, Quanjun, Yunfei Bai, Qinyu Ge, Shixin Zhou, Tian Wen, and Zuhong Lu. "Microarray-in-a-Tube for Detection of Multiple Viruses." Clinical Chemistry 53, no. 2 (2007): 188–94. http://dx.doi.org/10.1373/clinchem.2006.071720.

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Abstract Background: The detection of multiple viruses is important for pathogenic diagnosis and disease control. Microarray detection is a good method, but requires complex procedures for multiple virus detection. Methods: We developed a novel PCR assay, the microarray-in-a-tube system, which integrates multiple PCR processes and DNA microarrays for multiple virus detection. A 5 × 5 oligonucleotide microarray for detecting 4 respiratory tract viruses (severe acute respiratory syndrome–associated coronavirus, influenza A virus, influenza B virus, and enterovirus) with inner controls was arrang

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28

Dubrovina, A. I., and M. H. Alkordi. "Detection of «Telegram Rat» virus." Herald of Dagestan State Technical University. Technical Sciences 51, no. 1 (2024): 79–86. http://dx.doi.org/10.21822/2073-6185-2024-51-1-79-86.

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Objective. The aim of this study is to analyze the «Telegram Rat» virus, emphasizing the importance of awareness to effectively combat cyber threats and ensure security in the digital age.Methods. This paper used an analysis of the characteristics and distribution of «Telegram Rat» viruses. An example of analyzing the technical mechanisms of extortion on the example of «WAGNER GROUP» was given and the steps of virus elimination were formulated.Results. The acuality of the «Telegram Rat» virus problem and ways of its transmission are considered. Practical methods of threat detection and neutral

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29

Gambino, Giorgio, and Ivana Gribaudo. "Simultaneous Detection of Nine Grapevine Viruses by Multiplex Reverse Transcription-Polymerase Chain Reaction with Coamplification of a Plant RNA as Internal Control." Phytopathology® 96, no. 11 (2006): 1223–29. http://dx.doi.org/10.1094/phyto-96-1223.

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Анотація:

A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for simultaneous detection of nine grapevine viruses: Arabis mosaic virus, Grapevine fanleaf virus, Grapevine virus A, Grapevine virus B, Rupestris stem pitting-associated virus, Grapevine fleck virus, Grapevine leafroll-associated virus-1, -2, and -3, in combination with a plant RNA internal control used as an indicator of the effectiveness of RNA extraction and RT-PCR. Primers were designed from conserved regions of each virus and their specificity was confirmed by sequencing PCR products. Two plant total RNA

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30

Wu, Xinyan, Shuting Chen, Zixin Zhang, et al. "Development of Recombinase Polymerase Amplification Combined with Lateral Flow Strips for Rapid Detection of Cowpea Mild Mottle Virus." Plant Pathology Journal 39, no. 5 (2023): 486–93. http://dx.doi.org/10.5423/ppj.oa.02.2023.0033.

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Cowpea mild mottle virus (CPMMV) is a global plant virus that poses a threat to the production and quality of legume crops. Early and accurate diagnosis is essential for effective managing CPMMV outbreaks. With the advancement in isothermal recombinase polymerase amplification and lateral flow strips technologies, more rapid and sensitive methods have become available for detecting this pathogen. In this study, we have developed a reverse transcription recombinase polymerase amplification combined with lateral flow strips (RT-RPA-LFS) method for the detection of CPMMV, specifically targeting t

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31

Hirota, Ryuichi, Toru Murayama, Ryota Katsumi, et al. "Rapid virus detection using magnetic second harmonics of superparamagnetic iron oxide nanoparticles." AIP Advances 13, no. 2 (2023): 025144. http://dx.doi.org/10.1063/9.0000483.

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Virus detection methods based on nonlinear magnetic response of magnetic nanoparticles have been investigated, and magnetic detection methods using the third harmonic are widely applied, owing to their high sensitivity and short measurement time. This paper proposes a virus detection method based on the second harmonic because of its larger signal component. We found that the second harmonic signal is superior to the third harmonic signal for small nanobeads and a large change of the second harmonic signal in the signal-to-noise ratio (SNR) with nanobeads concentration. In addition, a virus de

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32

TAKU, ANIL, BALDEV R. GULATI, PAUL B. ALLWOOD, KERRIN PALAZZI, CRAIG W. HEDBERG, and SAGAR M. GOYAL. "Concentration and Detection of Caliciviruses from Food Contact Surfaces." Journal of Food Protection 65, no. 6 (2002): 999–1004. http://dx.doi.org/10.4315/0362-028x-65.6.999.

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Outbreaks of human Norwalk virus (NV) and Norwalk-like viruses often originate in food service establishments. No reliable method is available for the detection of these human caliciviruses on food contact surfaces. We describe a simple method for the detection of NV from stainless steel work surfaces using cultivable feline calicivirus (FCV) as a model. Stainless steel surfaces were artificially contaminated with known amounts of FCV, followed by its elution in a buffer solution. Three methods of virus elution were compared. In the first method, moistened cotton swabs or pieces of nylon filte

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33

Klestova, Z. S., A. K. Voronina, A. Yu Yushchenko, et al. "SURFACE PLASMON RESONANCE METHOD FOR DETECTION CHICKEN INFECTIOUS BRONCHITIS CORONAVIRUS." Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology 21, no. 2 (2020): 48–56. http://dx.doi.org/10.36359/scivp.2020-21-2.06.

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The article presents a new developed method, which is able to detect the chicken infectious bronchitis virus (IBV) antigen in real time in various buffer solutions, using the surface plasmon resonance (PPR) nanobiosensor of the Plasmon-6 device. The PPR method is hypersensitive to changes in external factors, including the interaction of antigen (coronavirus) and specific antibodies. If the interaction does not happen, the resonance occurs at other angular parameters of the position of the sensitive PPR element relative to the laser radiation. Therefore, the PPR method is becoming a new effect

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Saylan, Yeşeren, Özgecan Erdem, Serhat Ünal, and Adil Denizli. "An Alternative Medical Diagnosis Method: Biosensors for Virus Detection." Biosensors 9, no. 2 (2019): 65. http://dx.doi.org/10.3390/bios9020065.

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Infectious diseases still pose an omnipresent threat to global and public health, especially in many countries and rural areas of cities. Underlying reasons of such serious maladies can be summarized as the paucity of appropriate analysis methods and subsequent treatment strategies due to the limited access of centralized and equipped health care facilities for diagnosis. Biosensors hold great impact to turn our current analytical methods into diagnostic strategies by restructuring their sensing module for the detection of biomolecules, especially nano-sized objects such as protein biomarkers

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Hataya, Tatsuji, Alice Kazuko Inoue, and Eishiro Shikata. "A PCR-microplate hybridization method for plant virus detection." Journal of Virological Methods 46, no. 2 (1994): 223–36. http://dx.doi.org/10.1016/0166-0934(94)90105-8.

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Gervasi, Vincenzo, Andrea Marcon, Silvia Bellini, and Vittorio Guberti. "Evaluation of the Efficiency of Active and Passive Surveillance in the Detection of African Swine Fever in Wild Boar." Veterinary Sciences 7, no. 1 (2019): 5. http://dx.doi.org/10.3390/vetsci7010005.

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African swine fever (ASF) is one of the most severe diseases of pigs and has a drastic impact on pig industry. Wild boar populations play the role of ASF genotype II virus epidemiological reservoir. Disease surveillance in wild boar is carried out either by testing all the wild boar found sick or dead for virus detection (passive surveillance) or by testing for virus (and antibodies) all hunted wild boar (active surveillance). When virus prevalence and wild boar density are low as it happens close to eradication, the question on which kind of surveillance is more efficient in detecting the vir

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Chauhan, Ravendra P., Dulanjani Wijayasekara, Mark A. Webb, and Jeanmarie Verchot. "A Reliable and Rapid Multiplex RT-PCR Assay for Detection of Two Potyviruses and a Pararetrovirus Infecting Canna Plants." Plant Disease 99, no. 12 (2015): 1695–703. http://dx.doi.org/10.1094/pdis-02-15-0225-re.

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Canna plants are subject to serious virus diseases. The three most common viruses identified in canna plants are Bean yellow mosaic virus, Canna yellow mottle virus, and Canna yellow streak virus. Recent studies indicate that canna plants are commonly infected with more than one virus. Thus, the efficient control of these viruses in canna plants requires the availability of a reliable method for detecting mixed virus infection. This report presents a two-step multiplex reverse-transcription polymerase chain reaction (RT-PCR) that was developed to simultaneously detect two potyviruses and one p

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Wang, Yunpeng. "Comparative study of different detection method on the virus titers." Journal of Applied Virology 2, no. 3 (2013): 6. http://dx.doi.org/10.21092/jav.v2i3.19.

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<p>The counting of the virions plays a significant role in the study of virology. The detection methods of the virus titers can be mainly divided into two kinds: that based on the experimental animals and that on the cell culture technique. The former can be applied to those viruses that possess the experimental animal model, and the latter, according to the pathological advances of the cells, can be further divided into direct observation of the pathological advances and the plaque detection. When it comes to those viruses, that either have no CPE symptom after having infected the cells

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Bao, Hongmei, Yuhui Zhao, Yunhe Wang, et al. "Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus." BioMed Research International 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/525064.

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A novel influenza A (H7N9) virus has emerged in China. To rapidly detect this virus from clinical samples, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the detection of the H7N9 virus. The minimum detection limit of the RT-LAMP assay was 0.01 PFU H7N9 virus, making this method 100-fold more sensitive to the detection of the H7N9 virus than conventional RT-PCR. The H7N9 virus RT-LAMP assays can efficiently detect different sources of H7N9 influenza virus RNA (from chickens, pigeons, the environment, and humans). No cross-reactive amplification

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Qi, Ju, Jingna Han, Guoyu Niu, Zhenyu Liu, Xin Wang, and Yin Liu. "The development of ELISA Kit detecting Chikungunya Virus used a synthetic polypeptide chain." Journal of Applied Virology 3, no. 2 (2014): 31. http://dx.doi.org/10.21092/jav.v3i2.42.

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<p>Chikungunya fever was an acute vector-infectious disease caused by Chikungunya virus which outbreaks were distributed mostly in Africa, Indian Ocean, West Pacific Islands and South-East Asia. It was important to develop a rapid and accurate detection kit to control the Chikungunya virus transmission. The ELISA method was a valuable tool for rapid diagnosis of acute viral infections which had the merits of rapid, accurate, simple. In present study, a polypeptide chain of E2 protein was chosen and validated through computer protein model analysis, and the Chikungunya virus ELISA kit det

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Reyes Negrete, Heidy Stefania, Álvaro Sebastián Ron Mora, and Gabriela Paola Valenzuela Sánchez. "Serological and molecular methods for the detection of hepatitis B virus infections." Salud, Ciencia y Tecnología 5 (January 1, 2025): 1221. https://doi.org/10.56294/saludcyt20251221.

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Introduction: the hepatitis B virus has the ability to severely infect the liver and cause both acute and chronic infections. It belongs to the Hepadnaviridae family, is composed of partially double-stranded DNA, and contains four open reading frames (ORFs): ORF S (surface), C (core), P (polymerase), and X (HBx). The diagnosis is primarily based on detecting the surface antigen (HBsAg) and human antibodies against these antigens through immunological methods. Similarly, molecular methods such as PCR, qPCR, and LAMP are currently used, offering higher sensitivity and specificity. Objective: to

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Ushkalenko, Nikita D., Anna V. Ersh, Pavel V. Filatov, and Alexander G. Poltavchenko. "The rapid ELISA method for detection of orthopoxviruses." Problems of Virology 68, no. 3 (2023): 242–51. http://dx.doi.org/10.36233/0507-4088-178.

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Introduction. Following the successful eradication of smallpox, mass vaccination against this disease was discontinued in 1980. The unvaccinated population continues to be at risk of infection due to military use of variola virus or exposure to monkeypox virus in Africa and non-endemic areas. In cases of these diseases, rapid diagnosis is of great importance, since the promptness and effectiveness of therapeutic and quarantine measures depend on it. The aim of work is to develop a kit of reagents for enzyme-linked immunosorbent assay (ELISA) for fast and highly sensitive detection of ort

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Yin, Jingqi, Jin Cui, Hui Zheng, et al. "Implementation of RT-RAA and CRISPR/Cas13a for an NiV Point-of-Care Test: A Promising Tool for Disease Control." Viruses 17, no. 4 (2025): 483. https://doi.org/10.3390/v17040483.

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Nipah virus (NiV) is a severe zoonotic pathogen that substantially threatens public health. Pigs are the natural hosts of NiV and can potentially transmit this disease to humans. Establishing a rapid, sensitive, and accurate point-of-care detection method is critical in the timely identification of infected pig herds. In this study, we developed an NiV detection method based on reverse transcription–recombinase polymerase amplification (RT-RAA) and the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 13a (Cas13a) system for the precise detection of NiV. The

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Macharia, Edna W., Yatinder S. Binepal, Justus Onguso, Roy Kiambi, and Bramwel Wanjala. "Development and comparison of a loop mediated isothermal amplification assay for the rapid diagnosis of lumpy skin disease." Journal of Agriculture, Science and Technology 23, no. 3 (2024): 45–66. http://dx.doi.org/10.4314/jagst.v23i3.4.

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Lumpy skin disease virus is a poxvirus in the genus Capripoxvirus and is closely related to sheeppox virus and goatpox virus. It’s economically important in cattle and a notifiable disease by World Organization for Animal Health. Lumpy skin disease (LSD) is endemic in most parts of Africa with small-scale farmers experiencing the highest loss during outbreaks due to restricted animal trade and costly control and eradication measures. Serological methods of LSD detection are sensitive, inexpensive but can be laborious and time- consuming while, molecular methods such as Polymerase chain reactio

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Huang, Yi-Huei, Kuan-Yi Yu, Shou-Ping Huang, et al. "Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK." Diagnostics 10, no. 6 (2020): 403. http://dx.doi.org/10.3390/diagnostics10060403.

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The BK virus (BKV) is an emerging pathogen in immunocompromised individuals and widespread in the human population. Polymerase chain reaction is a simple and highly sensitive method for detecting BKV, but it is time consuming and requires expensive instruments and expert judgment. The lateral flow assay, a rapid, low-cost, minimal-labor, and easy-to-use diagnostic method, was successfully applied for pathogen detection. In this study, we used oligonucleotide probes to develop a simple and rapid sandwich-type lateral flow immunoassay for detecting BKV DNA within 45 minutes. The detection limit

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Zhai, Qi, Xia Zhou, Liyin Du, et al. "A Real-Time Recombinase Polymerase Amplification Assay for Specific Detection of Lumpy Skin Disease Virus." Veterinary Sciences 10, no. 10 (2023): 625. http://dx.doi.org/10.3390/vetsci10100625.

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Lumpy skin disease virus (LSDV) infection, accompanied by loss of hide quality, poor reproductive efficiency, consistent degenerative emaciation, and milk yield reduction of animals, causes severe economic implications in endemic zones. The heterologous attenuated goat pox (GTPV) vaccine (AV41 strain) was used in China to prevent LSDV infection. Only a few LSDV detection methods that distinguish LSDV from GTPV vaccine strains have been reported before. For simple, rapid, and specific detection of LSDV, the real-time recombinase polymerase amplification (RPA) method was established with the spe

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Simo, Fredy Brice Nemg, Felicity Jane Burt, and Nigel Aminake Makoah. "Chikungunya Virus Diagnosis: A Review of Current Antigen Detection Methods." Tropical Medicine and Infectious Disease 8, no. 7 (2023): 365. http://dx.doi.org/10.3390/tropicalmed8070365.

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Chikungunya is a mosquito-borne viral disease caused by the chikungunya virus (CHIKV). CHIKV is expanding at an alarming rate, potentially spreading and establishing endemicity in new areas where competent vectors are present. The dramatic spread of CHIKV in recent years highlights the urgent need to take precautionary measures and investigate options for control. It is crucial in developing nations where diagnostic tools are limited, and symptoms are similar to other prevalent diseases such as malaria and dengue. The most reliable method for diagnosing chikungunya virus is viral gene detectio

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Zhang, Mingzhe, Wujian Chen, Xi Chen, et al. "Multiplex Immunoassays of Plant Viruses Based on Functionalized Upconversion Nanoparticles Coupled with Immunomagnetic Separation." Journal of Nanomaterials 2013 (2013): 1–8. http://dx.doi.org/10.1155/2013/317437.

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A sensitive, specific and rapid method for the detection of three different kinds of plant viruses includingtomato ringspot virus(ToRSV),bean pod mottle virus(BPMV) andarabis mosaic virus(ArMV) was demonstrated using novel upconversion nanoparticles (UCNPs) as a fluorescence marker coupled with immunomagnetic separation. Magnetic nanoparticles (MNPs, ~100 nm) were coated with different antibodies were employed to capture and enrich the target viruses. Then antibody-conjugated UCNPs as signal probes were added to form sandwich complexes. This was followed by a fluorescence measurement using a 9

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Yan, Linjie, Yafen Song, Tianshu Zhai, et al. "Establishment of a Visual Gene Chip Method for the Simultaneous Detection of Seven Waterfowl Virus Pathogens." Viruses 17, no. 3 (2025): 358. https://doi.org/10.3390/v17030358.

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Goose parvovirus (GPV), duck enteritis virus (DEV), Muscovy duck parvovirus (MDPV), duck hepatitis A virus type 1 (DHAV-1), duck hepatitis A virus type 3 (DHAV-3), duck Tembusu virus (DTMUV), and novel duck reovirus (NDRV) are significant pathogens that spread extensively among waterfowl populations, causing economic losses for the waterfowl industry. In order to detect seven pathogens simultaneously, a visual gene chip for the detection of multiple waterfowl disease pathogens was developed in this study. The gene chip was capable of specifically amplifying GPV, DEV, MDPV, DHAV-1, –DHAV-3, DTM

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Gerloff, Nancy, Mark Mandelbaum, Hong Pang, et al. "Direct detection of polioviruses using a recombinant poliovirus receptor." PLOS ONE 16, no. 11 (2021): e0259099. http://dx.doi.org/10.1371/journal.pone.0259099.

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Polioviruses are positive-sense, single-stranded RNA picornaviruses and the principal cause of poliomyelitis. Global poliovirus surveillance has relied on poliovirus isolation in cells, which may take a minimum of 10 days, involves maintaining two cell lines, and propagates virus in high titers. With eradication underway, a major objective of the Global Polio Eradication Initiative (GPEI) is to develop culture-independent detection of polioviruses as an alternative method to complement the current virus isolation technique. A culture-independent method on poliovirus-positive stool suspensions

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