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Добірка наукової літератури з теми "Virus-induced enzymes"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 31 січня 2023

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Статті в журналах з теми "Virus-induced enzymes"

Knobil, Katharine, Augustine M. K. Choi, Gordon W. Weigand, and David B. Jacoby. "Role of oxidants in influenza virus-induced gene expression." American Journal of Physiology-Lung Cellular and Molecular Physiology 274, no. 1 (January 1, 1998): L134—L142. http://dx.doi.org/10.1152/ajplung.1998.274.1.l134.

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Influenza virus-induced epithelial damage may be mediated, in part, by reactive oxygen intermediates (ROIs). In this study, we investigated the role of ROIs in the influenza virus-induced gene expression of antioxidant enzymes and in the activation of nuclear factor-κB (NF-κB), an oxidant-sensitive transcriptional factor. Influenza virus infection increased production of intracellular ROIs in A549 pulmonary epithelial cells. Induction of manganese superoxide dismutase (MnSOD) mRNA correlated with increased MnSOD protein and enzyme activity. Influenza virus infection also activated NF-κB bindin

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Seo, Young-Jin, Celeste Blake, Stephen Alexander, and Bumsuk Hahm. "Sphingosine 1-Phosphate-Metabolizing Enzymes Control Influenza Virus Propagation and Viral Cytopathogenicity." Journal of Virology 84, no. 16 (June 2, 2010): 8124–31. http://dx.doi.org/10.1128/jvi.00510-10.

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ABSTRACT Sphingosine 1-phosphate (S1P)-metabolizing enzymes regulate the level of sphingolipids and have important biological functions. However, the effects of S1P-metabolizing enzymes on host defense against invading viruses remain unknown. In this study, we investigated the role of S1P-metabolizing enzymes in modulating cellular responses to influenza virus infection. Overexpression of S1P lyase (SPL), which induces the degradation of S1P, interfered with the amplification of infectious influenza virus. Accordingly, SPL-overexpressing cells were much more resistant than control cells to the

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Bornemann, Claus, and Hartmut Follmann. "Deoxyribonucleotide Synthesis in Phycovirus-Infected Green Algae. A New Virus-Induced Ribonucleotide Reductase." Zeitschrift für Naturforschung C 48, no. 1-2 (February 1, 1993): 113–18. http://dx.doi.org/10.1515/znc-1993-1-222.

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Infection of Chlorella-like green algae with freshwater phycoviruses is associated with a large and rapid demand for DNA precursors which cannot be met by the algal deoxyribonucleotide-synthesizing enzymes. We have demonstrated in these cells an up to ten-fold increase of the key enzyme, ribonucleotide reductase, 1-2 h post infection. The enzyme activity has been partially enriched from cell extracts. In vitro, it differs from that of uninfected algae in three characteristic parameters, viz. eight-fold higher resistance to millimolar hydroxyurea concentrations, much higher optimum concentratio

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Martínez-Costas, José, Claudia González-López, Vikram N. Vakharia та Javier Benavente. "Possible Involvement of the Double-Stranded RNA-Binding Core Protein ςA in the Resistance of Avian Reovirus to Interferon". Journal of Virology 74, № 3 (1 лютого 2000): 1124–31. http://dx.doi.org/10.1128/jvi.74.3.1124-1131.2000.

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ABSTRACT Treatment of primary cultures of chicken embryo fibroblasts with a recombinant chicken alpha/beta interferon (rcIFN) induces an antiviral state that causes a strong inhibition of vaccinia virus and vesicular stomatitis virus replication but has no effect on avian reovirus S1133 replication. The fact that avian reovirus polypeptides are synthesized normally in rcIFN-treated cells prompted us to investigate whether this virus expresses factors that interfere with the activation and/or the activity of the IFN-induced, double-stranded RNA (dsRNA)-dependent enzymes. Our results demonstrate

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Hsiang, Tien-Ying, Chen Zhao, and Robert M. Krug. "Interferon-Induced ISG15 Conjugation Inhibits Influenza A Virus Gene Expression and Replication in Human Cells." Journal of Virology 83, no. 12 (April 8, 2009): 5971–77. http://dx.doi.org/10.1128/jvi.01667-08.

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ABSTRACT The ubiquitin-like ISG15 protein, as well as its conjugating enzymes, is induced by type I interferons (IFNs). Experiments using ISG15 knockout (ISG15−/−) mice established that ISG15 and/or its conjugation inhibits the replication of influenza A virus. However, in contrast to the virus inhibition results for mice, the rates of virus replication in ISG15+/+ and ISG15−/− mouse embryo fibroblasts in tissue culture were similar. Here we focus on human tissue culture cells and on the effect of ISG15 and/or its conjugation on influenza A virus gene expression and replication in such cells.

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Choi, A. M., K. Knobil, S. L. Otterbein, D. A. Eastman, and D. B. Jacoby. "Oxidant stress responses in influenza virus pneumonia: gene expression and transcription factor activation." American Journal of Physiology-Lung Cellular and Molecular Physiology 271, no. 3 (September 1, 1996): L383—L391. http://dx.doi.org/10.1152/ajplung.1996.271.3.l383.

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The pathogenesis of influenza virus infections of the lungs is in part mediated by oxidative stress. Such infections might therefore be expected to induce expression of stress-response genes and genes encoding antioxidant enzymes and to activate transcriptional regulatory proteins. Mice (C57B1/6 and C3H/HeJ) were infected intranasally with influenza virus A/PR/8/34 (H1N1). Expression of the genes encoding the antioxidant enzymes manganese superoxide dismutase (Mn- SOD), indoleamine-2, 3-dioxygenase (IDO), heme oxygenase-1, and glutathione peroxidase were increased in the lungs of virus-infecte

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Knoester, Marga. "Virus-Induced Gene Expression for Enzymes of Ethylene Biosynthesis in Hypersensitively Reacting Tobacco." Molecular Plant-Microbe Interactions 8, no. 1 (1995): 177. http://dx.doi.org/10.1094/mpmi-8-0177.

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Staley, S., Marcela Smid, Sarah Dotters-Katz, and Elizabeth Stringer. "Epstein–Barr Virus-Induced Mononucleosis as an Imitator of Severe Preeclampsia." American Journal of Perinatology Reports 07, no. 01 (January 2017): e5-e7. http://dx.doi.org/10.1055/s-0036-1597265.

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Background In pregnancy, conditions presenting with hematologic abnormalities, transaminitis, and proteinuria pose diagnostic challenges in pregnancy. Case We present the case of an 18-year-old woman, G1P0, at 33 weeks' gestation with fever of unknown cause, who developed progressively elevated liver enzymes, proteinuria, and thrombocytopenia, due to Epstein–Barr virus (EBV) infection. Conclusion Acute infection with EBV should be included in the differential diagnosis of preeclampsia with severe features, particularly in the setting of fever. Supportive treatment and observation may prevent i

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Drecktrah, Daniel, and William J. Brown. "Phospholipase A2Antagonists Inhibit Nocodazole-induced Golgi Ministack Formation: Evidence of an ER Intermediate and Constitutive Cycling." Molecular Biology of the Cell 10, no. 12 (December 1999): 4021–32. http://dx.doi.org/10.1091/mbc.10.12.4021.

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Evidence has been presented both for and against obligate retrograde movement of resident Golgi proteins through the endoplasmic reticulum (ER) during nocodazole-induced Golgi ministack formation. Here, we studied the nocodazole-induced formation of ministacks using phospholipase A2(PLA2) antagonists, which have been shown previously to inhibit brefeldin A–stimulated Golgi-to-ER retrograde transport. Examination of clone 9 rat hepatocytes by immunofluorescence and immunoelectron microscopy revealed that a subset of PLA2antagonists prevented nocodazole-induced ministack formation by inhibiting

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Wijekoon, Champa P., and Peter J. Facchini. "Systematic knockdown of morphine pathway enzymes in opium poppy using virus-induced gene silencing." Plant Journal 69, no. 6 (December 28, 2011): 1052–63. http://dx.doi.org/10.1111/j.1365-313x.2011.04855.x.

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Дисертації з теми "Virus-induced enzymes"

Torok, Valeria Anna. "Biological and molecular variation among isolates of pea seed borne mosaic virus." Title page, contents and abstract only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09pht686.pdf.

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Corrigendum inserted at the back. Includes bibliographical references (leaves 133-158). Ch. 1. General introduction -- ch. 2. General materials and methods -- ch. 3. Biological characterisation of Australian PSbMV isolates -- ch. 4. Developing nucleic acid based diagnostics for PSbMV -- ch. 5. Detection of PSbMV isolates by RT-PCR and RFLP analysis -- ch. 6. Developing an internal control for PSbMV RT-PCR -- ch. 7. Molecular analysis of the PSbMV VPG -- ch. 8. PSbMV sequence and phylogenetic analysis -- ch. 9. General discussion Sixteen pea seed borne mosaic virus (PSbMV) isolates were collect

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Oh, Jun Seok. "Critical Roles of Cytomegalovirus-Induced Natural Killer Cells in Chronic Hepatitis C Virus Infection and Rituximab-Mediated Cancer Therapy." Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/36228.

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Natural Killer (NK) cells, members of the innate lymphoid cells (ILCs), are known to play an important role in the defense against foreign cells and abnormal host cells that have arisen due to viral infection or cancer inducing mutations. The typical immune response of NK cells involves the release of cytotoxic granules containing perforin and granzyme, and the secretion of immune-regulatory cytokines such as interferon gamma (IFN-γ). Unlike the adaptive lymphocytes such as T cells and B cells, NK cells do not require prior sensitization, enabling them to initiate an immune response much faste

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Black, Margaret E. "Structural and functional dissection of the vaccinia virus thymidine kinase enzyme." Thesis, 1991. http://hdl.handle.net/1957/37105.

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Thymidine kinase is a key enzyme in the nucleotide salvage pathway, catalyzing the production of dTMP from thymidine and ATP. In order to identify the structural features of the TK protein and/or primary amino acid sequences which contribute to the catalytic and regulatory activities of this enzyme, an in vitro transcription and translation system has been used in concert with protein engineering techniques for the production and phenotypic characterization of mutant and wild-type TK enzymes. Because of discrepancies in the literature regarding the quaternary structure of the VVTK, the native

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Hu, Chih-Chieh, and 胡智傑. "The role of proline synthesis enzymes under stresses via virus-induced gene silencing in tobacco." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/84132531497829537955.

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碩士 國立臺灣大學 農業化學研究所 96 Proline accumulation is wide spread phenomenon in stressed plants. Proline could be synthesized with Δ1-pyrroline-5-carboxylate synthetase (P5CS) from glutamate and with ornithine-δ-aminotransferase (OAT) from ornithine in plants. It dose don not know well about the role of P5CS and OAT in stressed plants. The aim of this thesis is to investigate which enzyme is more important for proline synthesis, and what is the possible function of these enzymes in response to abiotic stress. We used Tobacco mosaic virus (TMV) as vector with the insertion of proline metabo

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Bao, Kogan K. "Kinetic analysis of avian sarcoma virus integrase in the presteady-state." Thesis, 2002. http://hdl.handle.net/1957/30239.

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Integrase catalyzes insertion of a retroviral genome into the host chromosome. Following reverse transcription, integrase binds specifically to the ends of the duplex retroviral DNA, endonucleolytically cleaves two nucleotides from each 3'-end (the processing activity), and inserts these ends into the host DNA (the joining activity) in a concerted manner. Additionally, it has been observed that integrase can catalyze the removal of inserted viral ends (the disintegration activity) in vitro. Presteady-state experiments were performed using synapsed substrates to probe the processing reaction an

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Torok, Valeria Anna. "Biological and molecular variation among isolates of pea seed borne mosaic virus / Valeria Anna Torok." Thesis, 2001. http://hdl.handle.net/2440/21692.

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Corrigendum inserted at the back. Includes bibliographical references (leaves 133-158). xvi, 158 leaves : ill., col. map ; 30 cm. Sixteen pea seed borne mosaic virus (PSbMV) isolates were collected between 1995 and 1998. These isolates were biologically distinct yet serologically indistinguishable. The conclusion is that PSbMV is widespread and occurs at a low incidence in Australia. Reports sequence information on new isolates of PSbMV which has allowed genomic regions to be identified which distinguish PSbMV pathotypes and isolates; and, to the development of PSbMV nucleic acid hybr

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Kim, JuHyun. "Organization of the T4 dNTP synthetase complex at DNA replication sites." Thesis, 2005. http://hdl.handle.net/1957/29969.

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With respect to a multienzyme complex of deoxyribonucleoside triphosphate (dNTP) synthesis somehow juxtaposed with DNA replication sites, our laboratory has demonstrated the existence of a multienzyme complex in T4-infected E. coli, named the T4 dNTP synthetase complex, but the idea of direct linkage of dNTP synthesis to DNA replication and organization of the complex has not been well established. This study had two objectives. The first objective was to test the specific hypothesis that gp32, the single-stranded DNA binding protein encoded by gene 32, plays a role in recruiting enzymes of dN

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侯品全. "Investigation of the roles of polyamine biosynthetic enzymes in growth and developmental programs in Nicotiana benthamiana by virus-induced gene silencing." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/66899268862920599309.

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碩士 國立彰化師範大學 生物學系 101 Polyamines are implicated in a wide array of fundamental processes in plants such as adaptation and tolerance to environmental stresses. However, their roles in plant growth and developmental programs have not been fully elucidated. In this study, Nicotiana benthamiana was used as a model plants to investigate the roles of polyamine biosynthesis in developmental programs. Genes encoding polyamine biosynthesis enzymes were knocking down by using the VIGS (virus-induced gene silencing) strategies. Analysis of plant growth and development programs was carried o

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Wu, Wei-Zhi, and 吳偉誌. "Investigating the roles of polyamine-spermidine biosynthetic enzyme and nitric oxide in root growth of Nicotiana benthamiana by virus-induced gene silencing." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/r5ae94.

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碩士 國立彰化師範大學 生物學系 102 Polyamines and the biosynthesis of the corresponding enzymes have been implicated in environmental stresses and plant growth. Another NO (Nitric oxide) is a highly diffusible gaseous free radical involved in plant stress-related substance. However, the roles of polyamine and nitric oxide in root growth have not been fully elucidated. In this study, Nicotiana benthamiana was used as a model plants to investigate the roles of polyamine in root growth. Genes encoding polyamine biosynthesis enzymes were knocking down by using the virus-induced gene silencing (VIGS)

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Книги з теми "Virus-induced enzymes"

Morrison, J. M. Virus induced enzymes. Chichester, West Sussex, England: J. Wiley, 1991.

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Black, Margaret E. Structural and functional dissection of the vaccinia virus thymidine kinase enzyme. 1991.

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Частини книг з теми "Virus-induced enzymes"

Hodgson, H. J. F. "Viral hepatitis—clinical aspects." In Oxford Textbook of Medicine, 2452–60. Oxford University Press, 2010. http://dx.doi.org/10.1093/med/9780199204854.003.152101_update_002.

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There are five major hepatitis viruses—A, B, C, D, and E—with the clinical picture depending on the severity of the inflammation induced in the liver, and on whether the virus is cleared from the liver or persists long-term. Acute icteric hepatitis, characterized by jaundice and right upper quadrant abdominal tenderness, is the commonest clinically recognized consequence of infection. This is generally a self-limited condition with low mortality and complete recovery: only hepatitis B and C have the propensity to cause chronic viral hepatitis. Typically, hepatocellular enzyme levels in blood are prominently raised at the time of the onset of symptoms, whilst the serum alkaline phosphatase level is only slightly increased. Specific diagnosis is made by serological testing for particular viruses. Uncomplicated cases recover spontaneously; there is no proven therapy to enhance recovery, but alcohol and potentially hepatotoxic drugs should be withdrawn. Fulminant hepatic failure caused by viral hepatitis has 80% mortality and should be treated (if possible) by orthotopic liver transplantation....

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Тези доповідей конференцій з теми "Virus-induced enzymes"

Vlahos, Ross, Selcuk Yatmaz, Huei J. Seow, Rosa C. Gualano, Zi X. Wong, Peter J. Crack, Steven Bozinovski, and Gary P. Anderson. "The Antioxidant Enzyme Glutathione Peroxidase-1 (GPx-1) Reduces Influenza A Virus-Induced Lung Inflammation." In American Thoracic Society 2011 International Conference, May 13-18, 2011 • Denver Colorado. American Thoracic Society, 2011. http://dx.doi.org/10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a2754.

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Звіти організацій з теми "Virus-induced enzymes"

Meir, Shimon, Michael Reid, Cai-Zhong Jiang, Amnon Lers, and Sonia Philosoph-Hadas. Molecular Studies of Postharvest Leaf and Flower Abscission. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7696523.bard.

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Original objectives: Understanding the regulation of abscission competence by exploring the nature and function of auxin-related gene expression changes in the leaf and pedicelAZs of tomato (as a model system), was the main goal of the previously submitted proposal. We proposed to achieve this goal by using microarray GeneChip analysis, to identify potential target genes for functional analysis by virus-induced gene silencing (VIGS). To increase the potential of accomplishing the objectives of the previously submitted proposal, we were asked by BARD to show feasibility for the use of these two

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