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Journal articles on the topic '1/5-depleted'

Author: Grafiati

Published: 4 June 2021

Last updated: 18 February 2022

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1

Cui, Meiyan, Zhangzhen He, Nannan Wang, et al. "NaKV4O9·2H2O: a new 2D magnetic compound with a 1/5-depleted square lattice." Dalton Transactions 45, no. 12 (2016): 5234–39. http://dx.doi.org/10.1039/c5dt04745e.

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A new vanadate compound NaKV<sub>4</sub>O<sub>9</sub>·2H<sub>2</sub>O shows a typical 2D layered structure with a quite interesting 1/5-depleted square spin-lattice. Magnetic measurements demonstrate a spin-singlet ground state with a spin-gap in the system.

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2

Fukumoto, Yoshiyuki, and Akihide Oguchi. "Magnetization Process in Two-Dimensional Coupled Plaquette System on the 1/5-Depleted Square Lattice." Journal of the Physical Society of Japan 68, no. 11 (1999): 3655–61. http://dx.doi.org/10.1143/jpsj.68.3655.

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3

de Campos, Marcos Flavio, and Fernando J. G. Landgraf. "The Samarium Depleted Zone in SmCo₅ Magnets." Materials Science Forum 727-728 (August 2012): 169–74. http://dx.doi.org/10.4028/www.scientific.net/msf.727-728.169.

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After sintering, SmCo5magnets may present a surface layer depleted in Samarium. This happens because Samarium is much more susceptible for oxidation than cobalt. Besides, samarium has high vapour pressure. This Sm depleted zone is of the order of ~ 1 mm (in a 10 mm diameter magnet), and present oxygen content 1000 ppm higher than the center of the magnet. This layer is composed by SmCo(5+x), which presents low coercivity (1-2 kOe). The occurrence of the Sm depleted layer is associated with kinks in the 2ndquadrant of the hysteresis curve. After the removal of this layer, the hysteresis kinks d

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4

Rupprecht, H. D., P. Dann, V. P. Sukhatme, R. B. Sterzel, and D. L. Coleman. "Effect of vasoactive agents on induction of Egr-1 in rat mesangial cells: correlation with mitogenicity." American Journal of Physiology-Renal Physiology 263, no. 4 (1992): F623—F636. http://dx.doi.org/10.1152/ajprenal.1992.263.4.f623.

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The early growth response gene 1 (Egr-1) is a member of the family of immediate early response genes. Egr-1 encodes a nuclear phosphoprotein that binds a specific nonameric DNA sequence through three zinc-finger domains and functions as a transcriptional activator. We tested whether the vasoactive agents platelet-derived growth factor (PDGF), arginine vasopressin (AVP), serotonin (5-HT), and angiotensin II (ANG II) induced Egr-1 mRNA in cultured rat mesangial cells (MCs) and investigated the role of protein kinase C (PKC) in mediating the induction process. PDGF, AVP, and 5-HT induced Egr-1 mR

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5

Zhang, Su-Yun, Wen-Bin Guo, Ming Yang, et al. "A frustrated ferrimagnet Cu5(VO4)2(OH)4 with a 1/5 magnetization plateau on a new spin-lattice of alternating triangular and honeycomb strips." Dalton Transactions 44, no. 47 (2015): 20562–67. http://dx.doi.org/10.1039/c5dt03534a.

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6

Boritz, Eli, Brent E. Palmer, and Cara C. Wilson. "Human Immunodeficiency Virus Type 1 (HIV-1)-Specific CD4+ T Cells That Proliferate In Vitro Detected in Samples from Most Viremic Subjects and Inversely Associated with Plasma HIV-1 Levels." Journal of Virology 78, no. 22 (2004): 12638–46. http://dx.doi.org/10.1128/jvi.78.22.12638-12646.2004.

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ABSTRACT Diminished in vitro proliferation of human immunodeficiency virus type 1 (HIV-1)-specific CD4+ T cells has been associated with HIV-1 viremia and declining CD4+ T-cell counts during chronic infection. To better understand this phenomenon, we examined whether HIV-1 Gag p24 antigen-induced CD4+ T-cell proliferation might recover in vitro in a group of subjects with chronic HIV-1 viremia and no history of antiretroviral therapy (ART). We found that depletion of CD8+ cells from peripheral blood mononuclear cells (PBMC) before antigen stimulation was associated with a 6.5-fold increase in

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7

Wojtaszewski, Jørgen F. P., Christopher MacDonald, Jakob N. Nielsen, et al. "Regulation of 5′AMP-activated protein kinase activity and substrate utilization in exercising human skeletal muscle." American Journal of Physiology-Endocrinology and Metabolism 284, no. 4 (2003): E813—E822. http://dx.doi.org/10.1152/ajpendo.00436.2002.

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The metabolic role of 5′AMP-activated protein kinase (AMPK) in regulation of skeletal muscle metabolism in humans is unresolved. We measured isoform-specific AMPK activity and β-acetyl-CoA carboxylase (ACCβ) Ser221 phosphorylation and substrate balance in skeletal muscle of eight athletes at rest, during cycling exercise for 1 h at 70% peak oxygen consumption, and 1 h into recovery. The experiment was performed twice, once in a glycogen-loaded (glycogen concentration ∼900 mmol/kg dry wt) and once in a glycogen-depleted (glycogen concentration ∼160 mmol/kg dry wt) state. At rest, plasma long-ch

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8

Grignard, Elise, Yann Guéguen, Stéphane Grison, Jean-Marc A. Lobaccaro, Patrick Gourmelon, and Maâmar Souidi. "Contamination with Depleted or Enriched Uranium Differently Affects Steroidogenesis Metabolism in Rat." International Journal of Toxicology 27, no. 4 (2008): 323–28. http://dx.doi.org/10.1080/10915810802367057.

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Uranium is a naturally occurring heavy metal found in the Earth’s crust. It is an alpha-emitter radioactive element from the actinide group that presents both radiotoxicant and chemotoxicant properties. Some studies revealed that uranium could affect the reproductive system. To distinguish chemical versus radiological effects of uranium on the metabolism of the steroids in the testis, rats were contaminated via their drinking water with depleted or enriched uranium. Animals were exposed to radionuclides for 9 months at a dose of 40 mg/L (560 Bq/L for depleted uranium, 1680 Bq/L for enriched ur

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9

Alba, Raul, Angela C. Bradshaw, Lynda Coughlan, et al. "Biodistribution and retargeting of FX-binding ablated adenovirus serotype 5 vectors." Blood 116, no. 15 (2010): 2656–64. http://dx.doi.org/10.1182/blood-2009-12-260026.

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AbstractA major limitation for adenoviral transduction in vivo is the profound liver tropism of adenovirus type 5 (Ad5). Recently, we demonstrated that coagulation factor X (FX) binds to Ad5-hexon protein at high affinity to mediate hepatocyte transduction after intravascular delivery. We developed novel genetically FX-binding ablated Ad5 vectors with lower liver transduction. Here, we demonstrate that FX-binding ablated Ad5 predominantly localize to the liver and spleen 1 hour after injection; however, they had highly reduced liver transduction in both control and macrophage-depleted mice com

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10

Nakamura, Shigefumi, David B. Averill, Mark C. Chappell, Debra I. Diz, K. Bridget Brosnihan, and Carlos M. Ferrario. "Angiotensin receptors contribute to blood pressure homeostasis in salt-depleted SHR." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 284, no. 1 (2003): R164—R173. http://dx.doi.org/10.1152/ajpregu.00210.2002.

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This study evaluated the contribution of angiotensin peptides acting at various receptor subtypes to the arterial pressure and heart rate of adult 9-wk-old male conscious salt-depleted spontaneously hypertensive rats (SHR). Plasma ANG II and ANG I in salt-depleted SHR were elevated sevenfold compared with peptide levels measured in sodium-replete SHR, whereas plasma ANG-(1–7) was twofold greater in salt-depleted SHR compared with salt-replete SHR. Losartan (32.5 μmol/kg), PD-123319 (0.12 μmol · kg−1 · min−1), [d-Ala7]ANG-(1–7) (10 and 100 pmol/min), and a polyclonal ANG II antibody (0.08 mg/mi

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11

Freeman, Lisa M., Eugene Petcu, Robert Smith, et al. "Depletion of Host CD122+ Cells Facilitates Widespread Skeletal Multiple Myeloma Engraftment in NOD/SCID Recipients." Blood 112, no. 11 (2008): 2734. http://dx.doi.org/10.1182/blood.v112.11.2734.2734.

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Abstract To facilitate human multiple myeloma (MM) engraftment into NOD/SCID recipients, mice were depleted of CD122+ cells (NK and myeloid cells) by antibody-mediated ablation prior to transplantation with the MM cell lines (RPMI8226, RPMI8226-TGL or U226). The MM engraftment, skeletal MM distribution, osteolysis, lambda chain paraprotein and associating disease symptoms in CD122+ cell-depleted and CD122+ cellreplete mice were compared. The CD122+ cell-depleted mice engrafted at a significantly higher frequency with human CD38+, CD56+, PCA-1+ and CD138+ cells. In the CD122+ cell-depleted mice

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12

Hossain, Mohammad S., Cynthia R. Giver, and Ned Waller. "Homeostatic Expansion of Donor FoxP3+ CD25+CD4+ T Cells in Recipients of CD4+ Cells Depleted Allogeneic Hematopoietic Graft." Blood 108, no. 11 (2006): 3207. http://dx.doi.org/10.1182/blood.v108.11.3207.3207.

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Abstract Background: In allogeneic BMT patients, the presence of allo-reactive donor CD4+ T cells in the graft were reported to be the primary cause of GvHD. Moreover, donor T-cells are required to promote the stem cell engraftment and to decrease the disease relapse. A number of studies also reported that a subset of CD4+CD25+ T cells usually generated de novo from the thymus that expressed FoxP3 regulate the T cells allo-reactivity in vivo. Thus, to establish a therapeutically useful adoptive T-cells immunotherapy, we depleted the CD4+ T cells from the graft and transplanted along with T cel

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13

Ahern, Deborah A., and Madeleine E. Mitchell. "Liver function in protein-energy malnutrition measured by cinnamic acid tolerance and benzoic acid tolerance: effect of carnitine supplementation." British Journal of Nutrition 61, no. 2 (1989): 209–21. http://dx.doi.org/10.1079/bjn19890110.

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1. Rats fed on a protein-depleted diet for 8 weeks were repleted for 5 weeks on high-protein (HP), high-protein+20 g DL-carnitine/kg (HP +C), or low-protein + 20 g DL-carnitine/kg (LP +C) diets. At 4 and 8 weeks of depletion, and 1 and 5 weeks of repletion, rats from each treatment group were given a benzoic acid tolerance test (BATT) or a cinnamic acid tolerance test (CATT) as a measure of liver function.2. BATT and CATT measured the molar percentage of a test dose (1 mmol/kg body-weight) of benzoic acid or cinnamic acid excreted in the urine as hippuric acid within 24 h. Liver weight, liver

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14

Bell, J. G., J. W. Adron, and C. B. Cowey. "Effect of selenium deficiency on hydroperoxide-stimulated release of glutathione from isolated perfused liver of rainbow trout (Salmo gairdneri)." British Journal of Nutrition 56, no. 2 (1986): 421–28. http://dx.doi.org/10.1079/bjn19860122.

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1. Duplicate groups of rainbow trout (Salmo gairdneri) were each given partially purified diets which were either adequate or depleted in selenium for 40 weeks.2. Although there was no significant difference in weight gain, liver Se concentration was significantly lower in fish given the deficient diet.3. Glutathione (GSH) peroxidase (EC 1. 11. 1. 9) activity was significantly reduced in liver of Se-deficient fish but a differential assay did not indicate the presence of a non-Se-dependent GSH peroxidase activity, although liver GSH S-transferase (EC 2. 5. 1. 18) was significantly increased.4.

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15

Lee, Chang-Jin, Seon-Hwa Hong, Min-Ji Yoon, et al. "Endometrial profilin 1: a key player in embryo-endometrial crosstalk." Clinical and Experimental Reproductive Medicine 47, no. 2 (2020): 114–21. http://dx.doi.org/10.5653/cerm.2019.03454.

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Objective: Despite extensive research on implantation failure, little is known about the molecular mechanisms underlying the crosstalk between the embryo and the maternal endometrium, which is critical for successful pregnancy. Profilin 1 (PFN1), which is expressed both in the embryo and in the endometrial epithelium, acts as a potent regulator of actin polymerization and the cytoskeletal network. In this study, we identified the specific role of endometrial PFN1 during embryo implantation.Methods: Morphological alterations depending on the status of PFN1 expression were assessed in PFN1-deple

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16

Sugita, Kenji, Kazuo Hosoi, Yoshimi Shioda, and Takao Ueha. "Effects of Ca2+ and Mg2+ on ATP-dependent 45Ca2+ influx in A-431 human epidermoidal carcinoma cells." Biochemistry and Cell Biology 69, no. 1 (1991): 29–35. http://dx.doi.org/10.1139/o91-004.

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Upon stimulation with 10−6–10−3 M ATP, A-431 human epidermoidal carcinoma cells incorporated radioactive calcium from their medium in a temperature-dependent manner. The rate of incorporation of 45Ca2+ was rapid for the initial 5 min, but decreased immediately thereafter. The preincubation of cells for 2 h in medium depleted of both Ca2+ and Mg2+ abolished the ATP-dependent 45Ca2+ incorporation, irrespective of whether or not the subsequent incubation medium contained Mg2+ ions. ATP-dependent 45Ca2+ incorporation could be restored by a second preincubation (1 h) in medium containing 1 mM Mg2+,

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17

Shirley, D. G., S. J. Walter, and R. J. Unwin. "Mechanism of the Impaired Natriuretic Response to Frusemide during Sodium Depletion: A Micropuncture Study in Rats." Clinical Science 91, no. 3 (1996): 299–305. http://dx.doi.org/10.1042/cs0910299.

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1. The nephron sites involved in the blunted natriuretic response to frusemide during sodium depletion were investigated using micropuncture techniques in anaesthetized rats. 2. Glomerular filtration rate was lower, and fractional sodium reabsorption in the proximal convoluted tubule higher, in sodium-depleted than in sodium-replete rats. Consequently, sodium delivery to the loop of Henle was reduced (by approximately 35%) in the sodium-depleted animals. Intravenous frusemide (2.5 mg h−1 kg−1; urinary water and electrolyte losses replaced) had no effect on glomerular filtration rate or proxima

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18

Foley, J. M., S. J. Harkema, and R. A. Meyer. "Decreased ATP cost of isometric contractions in ATP-depleted rat fast-twitch muscle." American Journal of Physiology-Cell Physiology 261, no. 5 (1991): C872—C881. http://dx.doi.org/10.1152/ajpcell.1991.261.5.c872.

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ATP was depleted to inosine 5'-monophosphate (IMP) in gastrocnemius muscles of anesthetized rats during two consecutive bouts of intermittent tetanic stimulation (each 1 Hz at 100 Hz, 100-ms duration, for 3 min) separated by 5 min. During a subsequent 75-min recovery period, resynthesis of adenine nucleotide from IMP was inhibited in one group of muscles with hadacidin (N-formyl-N-hydroxyaminoacetate, total dose 300 mg/kg ip), an inhibitor of adenylosuccinate synthase. In vivo 31P-nuclear magnetic resonance spectra and analysis of muscle extracts confirmed that ATP was reduced by over 30% in h

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19

Platzer, Hadrian, Timo A. Nees, Tobias Reiner, et al. "Impact of Mononuclear Cell Infiltration on Chondrodestructive MMP/ADAMTS Production in Osteoarthritic Knee Joints—An Ex Vivo Study." Journal of Clinical Medicine 9, no. 5 (2020): 1279. http://dx.doi.org/10.3390/jcm9051279.

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Progressive loss of joint function in osteoarthritis (OA) is driven by degenerative and inflammatory processes and their complex interaction. Decoding the link between degeneration and inflammation is one of the most exciting approaches in understanding OA pathophysiology and holds the promise to open new therapeutic avenues. The overarching goal of this project was to analyze the impact of mononuclear cells (MNC) on enzymatic chondrodestructive processes (MMP/ADAMTS) in OA. Synovial membrane (SM), articular cartilage (AC) and peripheral blood (PB) were obtained from a total of 21 patients wit

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20

Raja'a, Y. A., S. M. Sulaiman, and J. S. Mubarak. "Nutritional status of Yemeni schoolchildren in Al-Mahweet Governorate." Eastern Mediterranean Health Journal 7, no. 1-2 (2001): 204–10. http://dx.doi.org/10.26719/2001.7.1-2.204.

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The nutritional status of 948 children selected randomly from Al-Mahweet Governorate schools was investigated. Age range was 5-18 years with a mean of 10.6 +/- 2.8 years. Among the children, 3.4% had depleted iron and 43.4% had below average skin-fold thickness. Approximately half of the children were either stunted or chronically underweight and 1 child in 20 was underfed. Depleted fat stores affected two-fifths of the children and approximately one-fifth were anaemic. Urban residents scored significantly higher on nutritional parameters than rural children. Serum ferritin levels were signifi

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21

Kernan, NA, NH Collins, L. Juliano, T. Cartagena, B. Dupont, and RJ O'Reilly. "Clonable T lymphocytes in T cell-depleted bone marrow transplants correlate with development of graft-v-host disease." Blood 68, no. 3 (1986): 770–73. http://dx.doi.org/10.1182/blood.v68.3.770.770.

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Abstract Early clinical trials using T lymphocyte-depleted human marrow for transplantation have reported that such grafts reduce, to varying degrees, both the incidence and the severity of graft-v-host disease (GVHD). However, to date, no clear estimates have been made as to what degree of T cell depletion is necessary to prevent GVHD in every case. To address this problem, we used a limiting dilution assay (LDA) to quantitate residual clonable T lymphocytes in human T cell-depleted bone marrow in 31 HLA-identical transplants for leukemia. The number of phytohemagglutinin -interleukin 2-respo

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22

Kernan, NA, NH Collins, L. Juliano, T. Cartagena, B. Dupont, and RJ O'Reilly. "Clonable T lymphocytes in T cell-depleted bone marrow transplants correlate with development of graft-v-host disease." Blood 68, no. 3 (1986): 770–73. http://dx.doi.org/10.1182/blood.v68.3.770.bloodjournal683770.

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Early clinical trials using T lymphocyte-depleted human marrow for transplantation have reported that such grafts reduce, to varying degrees, both the incidence and the severity of graft-v-host disease (GVHD). However, to date, no clear estimates have been made as to what degree of T cell depletion is necessary to prevent GVHD in every case. To address this problem, we used a limiting dilution assay (LDA) to quantitate residual clonable T lymphocytes in human T cell-depleted bone marrow in 31 HLA-identical transplants for leukemia. The number of phytohemagglutinin -interleukin 2-responsive T l

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23

Buendía, Antonio J., Roberto Montes de Oca, Jose A. Navarro, Joaquín Sánchez, Francisco Cuello, and Jesús Salinas. "Role of Polymorphonuclear Neutrophils in a Murine Model of Chlamydia psittaci-Induced Abortion." Infection and Immunity 67, no. 5 (1999): 2110–16. http://dx.doi.org/10.1128/iai.67.5.2110-2116.1999.

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ABSTRACT To assess the role of polymorphonuclear neutrophils (PMNs) inChlamydia psittaci infection in a pregnant mouse model, pregnant and nonpregnant Swiss OF1 mice were depleted of PMNs by treatment with the RB6-8C5 monoclonal antibody before intraperitoneal infection with C. psittaci serotype 1. Nondepleted mice served as infection controls. Depleted mice aborted earlier and had a much higher mortality rate than nondepleted mice. Bacteriological analysis showed that the number of chlamydiae isolated from the spleens of depleted mice at 5 and 7 days postinfection was 100 times greater than t

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24

Engler, R. L., M. D. Dahlgren, D. D. Morris, M. A. Peterson, and G. W. Schmid-Schonbein. "Role of leukocytes in response to acute myocardial ischemia and reflow in dogs." American Journal of Physiology-Heart and Circulatory Physiology 251, no. 2 (1986): H314—H323. http://dx.doi.org/10.1152/ajpheart.1986.251.2.h314.

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Recent evidence indicates that leukocytes (LEU) are large, stiff, viscous cells that naturally adhere to vascular endothelium. Their broad role in the early myocardial microvascular response to acute ischemia was suggested by 1) the role of leukocyte capillary plugging in the no-reflow phenomenon, 2) resistance increases in skeletal muscle with LEU infusions, and 3) salvage of ischemic myocardium by anti-LEU agents. We perfused the coronary circulation under matched, controlled conditions with whole blood or granulocyte-depleted whole blood. During 1 h of ischemia (left anterior descending occ

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25

Boulad, Farid, Nancy A. Kernan, Peter G. Steinherz, et al. "Allogeneic Hematopoietic Stem Cell Transplantation (HSCT) for the Treatment of Children with Very High Risk (VHR) Acute Lymphoblastic Leukemia (ALL) In First Remission (CR1)." Blood 116, no. 21 (2010): 3546. http://dx.doi.org/10.1182/blood.v116.21.3546.3546.

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Abstract Abstract 3546 Between August 1988 and December 2009, 40 pediatric patients (pts) received an allogeneic HSCT for the treatment of very high risk ALL in CR1. They were 22 males and 18 females, aged 0.8 –19.2 years (median 10.0). Thirty eight patients had very high risk features: high white cell count greater than 200,000/mm3 (N=7), hypodiploidy (N=6), including double hypodiploidy (N=3), high risk cytogenetics with t(9;22) (N=14) or Mixed Lineage Leukemia (MLL) (N=3), and induction failure (N=23). Induction failure was defined morphologically by a single marrow aspirate revealing great

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26

Miklos, David B., Katherine H. Miller, Haesook T. Kim, et al. "Allogeneic B Cell Response to H-Y Minor Histocompatibility Antigens after Donor Lymphocyte Infusion Correlates with Disease Response." Blood 104, no. 11 (2004): 296. http://dx.doi.org/10.1182/blood.v104.11.296.296.

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Abstract Donor lymphocyte infusion (DLI) can induce remission in many patients who relapse after allogeneic hematopoietic stem cell transplantation (HSCT). We have previously demonstrated that male HSCT patients with female donors frequently develop high-titer antibody responses to H-Y antigens that correlate with disease remission. DLI administered 6 months after T cell depleted HSCT results in a five-fold increase in peripheral B cell numbers. We sought to determine whether allogeneic B cell responses develop after DLI. We expressed 5 recombinant H-Y proteins (DBY, UTY, ZFY, RPS4Y and EIF1AY

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Gu, Weifeng, Rebecca L. Hurto, Anita K. Hopper, Elizabeth J. Grayhack, and Eric M. Phizicky. "Depletion of Saccharomyces cerevisiae tRNAHis Guanylyltransferase Thg1p Leads to Uncharged tRNAHis with Additional m5C." Molecular and Cellular Biology 25, no. 18 (2005): 8191–201. http://dx.doi.org/10.1128/mcb.25.18.8191-8201.2005.

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ABSTRACT The essential Saccharomyces cerevisiae tRNAHis guanylyltransferase (Thg1p) is responsible for the unusual G−1 addition to the 5′ end of cytoplasmic tRNAHis. We report here that tRNAHis from Thg1p-depleted cells is uncharged, although histidyl tRNA synthetase is active and the 3′ end of the tRNA is intact, suggesting that G−1 is a critical determinant for aminoacylation of tRNAHis in vivo. Thg1p depletion leads to activation of the GCN4 pathway, most, but not all, of which is Gcn2p dependent, and to the accumulation of tRNAHis in the nucleus. Surprisingly, tRNAHis in Thg1p-depleted cel

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Hofman, W. F., and I. C. Ehrhart. "Albumin attenuation of oleic acid edema in dog lung depleted of blood components." Journal of Applied Physiology 58, no. 6 (1985): 1949–55. http://dx.doi.org/10.1152/jappl.1985.58.6.1949.

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Circulating fatty acids are normally transported principally bound to serum albumin. We examined whether administering oleic acid (OA) in a concentrated albumin solution would attenuate its edemogenic potential in the isolated dog lung lobe perfused with a solution nearly depleted of blood cellular and protein components. The isolated ventilated lower left lobe (LLL) was perfused (7.3 +/- 0.6 ml X min-1 X g LLL-1) with a balanced salt solution containing 6% dextran and approximately 10% serum (vol/vol). Hourly weight gain, net LLL weight gain, and wet-to-dry weight ratio (W/D) were used as ind

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29

van Hardeveld, C., V. J. Schouten, A. Muller, E. T. van der Meulen, and G. Elzinga. "Exposure of energy-depleted rat trabeculae to low pH improves contractile recovery: role of calcium." American Journal of Physiology-Heart and Circulatory Physiology 268, no. 4 (1995): H1510—H1520. http://dx.doi.org/10.1152/ajpheart.1995.268.4.h1510.

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The beneficial effect of low pH during cardiac ischemia on reperfusion injury has often been attributed to its energy-saving effect due to inhibition of contraction. The role of low pH on Ca2+ accumulation and muscle tension was assessed in energy-depleted tissue by changing the pH of the medium from 7.4 to 6.2 at onset of rigor development during metabolic inhibition (MI), i.e., in the energy-depleted phase. Cytosolic free Ca2+ ([Ca2+]i) and intracellular H+ (pHi) were measured in rat trabeculae at 20 degrees C with fura 2 and 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein, respectively, and

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30

Andersson, U. B., L. A. Neymark, and K. Billström. "Petrogenesis of Mesoproterozoic (Subjotnian) rapakivi complexes of central Sweden: implications from U–Pb zircon ages, Nd, Sr and Pb isotopes." Transactions of the Royal Society of Edinburgh: Earth Sciences 92, no. 3 (2001): 201–28. http://dx.doi.org/10.1017/s0263593300000237.

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ABSTRACTU-Pb zircon geochronology of Mesoproterozoic (Subjotnian) rapakivi complexes in central Sweden yields: 1526 ± 3 Ma (Mullnäset), 1524 ± 3 Ma (Mårdsjö), 1520 ± 3 Ma (Nordsjö) and 1497 ± 6 Ma (Rödön). Together with complexes further S in Sweden, they constitute the westernmost, youngest (1·53−1·47 Ga) belt of rapakivi magmatism in the Fennoscandian shield.The low initial εNd values (−8·9 to −4·8) of all studied Subjotnian basic, intermediate and silicic rocks, require an input from an old (Archaean) low-radiogenic source component, as evidence for Palaeoproterozoic protoliths in the age r

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31

Ay, Binnaz, Y. S. Prakash, Christina M. Pabelick, and Gary C. Sieck. "Store-operated Ca2+ entry in porcine airway smooth muscle." American Journal of Physiology-Lung Cellular and Molecular Physiology 286, no. 5 (2004): L909—L917. http://dx.doi.org/10.1152/ajplung.00317.2003.

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Ca2+ influx triggered by depletion of sarcoplasmic reticulum (SR) Ca2+ stores [mediated via store-operated Ca2+ channels (SOCC)] was characterized in enzymatically dissociated porcine airway smooth muscle (ASM) cells. When SR Ca2+ was depleted by either 5 μM cyclopiazonic acid or 5 mM caffeine in the absence of extracellular Ca2+, subsequent introduction of extracellular Ca2+ further elevated [Ca2+]i. SOCC was insensitive to 1 μM nifedipine- or KCl-induced changes in membrane potential. However, preexposure of cells to 100 nM–1 mM La3+ or Ni2+ inhibited SOCC. Exposure to ACh increased Ca2+ inf

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Lesnikova, Marina, Alla Nikitine, Lina Pogosov, Richard A. Nash, and George E. Georges. "Peripheral CD4+CD25+ Regulatory T Cells (Treg) Block Alloreactive Host Anti-Donor T Cells in Canine Mixed Hematopoietic Chimeras." Blood 106, no. 11 (2005): 3101. http://dx.doi.org/10.1182/blood.v106.11.3101.3101.

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Abstract CD4+CD25+ Treg cells may be important immune regulators for the maintenance of immune tolerance after allogeneic hematopoietic cell transplantation (HCT). In the well-established canine model of nonmyeloablative HCT, stable mixed donor/host hematopoietic chimerism persists for the lifespan of the recipient following 2 Gray total body irradiation, dog leukocyte antigen (DLA)-identical bone marrow transplantation and combined postgrafting immunosuppression with mycophenolate mofetil and cyclosporine for 4 and 5 weeks, respectively. We hypothesized that CD4+CD25+ Treg are an important co

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Ralph, P., I. Nakoinz, and D. Rennick. "Role of interleukin 2, interleukin 4, and alpha, beta, and gamma interferon in stimulating macrophage antibody-dependent tumoricidal activity." Journal of Experimental Medicine 167, no. 2 (1988): 712–17. http://dx.doi.org/10.1084/jem.167.2.712.

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Pretreatment of murine peritoneal exudate macrophages with 1-5 U/ml rIFN-gamma or rIL-2, or higher concentrations of IFN-alpha or IFN-beta greatly stimulated ADCC to Rl lymphoma targets. The assay was direct counting of viable target cells after 9 and 24 h using an E/T ratio of 5:1. 2d of pretreatment was optimal for enhancing ADCC. rIL-4 was inactive and IL-4-depleted Con A-induced spleen lymphokine retained its ADCC-stimulating activity. Antibody to IFN-gamma blocked the ADCC-promoting effect of the lymphokine, suggesting a major role for this factor.

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34

Bidet, M., J. Merot, M. Tauc, and P. Poujeol. "Na+-H+ exchanger in proximal cells isolated from kidney. II. Short-term regulation by glucocorticoids." American Journal of Physiology-Renal Physiology 253, no. 5 (1987): F945—F951. http://dx.doi.org/10.1152/ajprenal.1987.253.5.f945.

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The purpose of this study was to investigate the acute regulation by glucocorticoid of the Na+-H+ exchanger in isolated renal proximal cells of the rabbit. The changes of intracellular pH (pHi) were determined in a bicarbonate-free buffer by the use of a fluorescent pH probe that may be trapped intracellularly, 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF). The activity of the Na+-H+ exchanger was estimated by measuring the Na+-induced H+ efflux in BCECF-loaded cells acid loaded with nigericin in choline medium. The uptake of 1.5 mM 22Na was also studied in Na+-depleted cells. Acute appl

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Bhutada, A., and F. Ismail-Beigi. "Serum and growth factor induction of Na(+)-K(+)-ATPase subunit mRNAs in Clone 9 cells: role of protein kinase C." American Journal of Physiology-Cell Physiology 261, no. 4 (1991): C699—C707. http://dx.doi.org/10.1152/ajpcell.1991.261.4.c699.

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In a previous study, we found that addition of serum to confluent Clone 9 cells, a nontransformed rat liver cell line, increased the abundance of mRNA alpha 1 and mRNA beta 1 at 3 h by 2- and 2.7-fold, respectively [Bhutada et al. Am. J. Physiol. 258 (Cell Physiol. 27): C1044-C1050, 1990]. We now report that exposure of these cells to 160 nM 12-O-tetradecanoylphorbol 13-acetate (TPA) for 6 h increases mRNA alpha 1 and mRNA beta 1 by 1.7 +/- 0.2- and 2.1 +/- 0.3-fold, respectively. Incubation in the presence of 160 nM TPA for 24 h reduced high-affinity phorbol dibutyrate-binding sites [dissocia

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36

Boulad, Farid, Ann Jakubowski, Hugo Castro-Malaspina, et al. "A Phase II Trial of Busulfan, Melphalan, and Fludarabine followed by Allogeneic T-Cell Depleted Hematopoietic Stem Cell Transplants from HLA-Identical, or HLA-Non Identical Related or Unrelated Donors for the Treatment of Advanced Hematopoietic Malignancies." Blood 104, no. 11 (2004): 1822. http://dx.doi.org/10.1182/blood.v104.11.1822.1822.

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Abstract Twenty five patients have been enrolled on this trial to date. There were 14 males and 11 females aged 0.6–54 years. Patients’ diagnoses and stage included: NHL in CR2 or refractory (n=2), AML (n=7), including 5 pts with secondary AML, ALL > CR3 (n=4), CML in CP2 (N=1) and high risk MDS (n=11) including 5 pts with secondary MDS. Eight pts had a matched related donor, 14 pts an unrelated donor and 3 pts a mismatched related donor. Cytoreduction consisted of busulfan (Bu) (0.8–1 mg/Kg/dose x 10 doses), melphalan (Mel) (70 mg/Kg/day x 2) and fludarabine (Flu) (25 mg/m2/day x 5). Graft

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37

Johnston, Swan, and Corte. "Substrate Utilization and Work Efficiency during Submaximal Exercise in Vitamin C Depleted-Repleted Adults." International Journal for Vitamin and Nutrition Research 69, no. 1 (1999): 41–44. http://dx.doi.org/10.1024/0300-9831.69.1.41.

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A placebo-controlled, depletion-repletion protocol was utilized to examine the effect of vitamin C status on substrate utilization during a 90 min walk at 50% maximal oxygen consumption (VO2max). Nine vitamin C depleted subjects (plasma vitamin C < 28 mumol/L) agreed to participate in the 5-week study (aged, 27.6 ± 2.5 years , mean ± SE; 5 females, 4 males). Subjects were apparently healthy but unaware of their vitamin C status. Prior to the experimental period, VO 2max was measured using open-circuit spirometry during a graded walking protocol. Subjects ingested a placebo capsule daily dur

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38

Saito, H., T. Ishihara, H. Suzuki, and T. Watanabe. "Production and characterization of a murine monoclonal antibody against a heavy chain of Hageman factor (factor XII)." Blood 65, no. 5 (1985): 1263–68. http://dx.doi.org/10.1182/blood.v65.5.1263.1263.

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Abstract A murine hybridoma cell line that produces a monoclonal antibody to human Hageman factor (HF, factor XII) is described. The antibody (P 5–2– 1) consists of mouse IgG2b heavy chains and lambda light chains, selectively neutralizes HF procoagulant activity, and prevents the proteolytic cleavage of HF during contact activation in plasma. When HF is exposed to P 5–2–1 before the absorption of HF to kaolin, HF procoagulant activity is markedly inhibited. In contrast, P 5–2–1 does not interfere with HF activity after the adsorption of HF to kaolin. P 5–2–1 does not inactivate the prekallikr

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39

Saito, H., T. Ishihara, H. Suzuki, and T. Watanabe. "Production and characterization of a murine monoclonal antibody against a heavy chain of Hageman factor (factor XII)." Blood 65, no. 5 (1985): 1263–68. http://dx.doi.org/10.1182/blood.v65.5.1263.bloodjournal6551263.

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A murine hybridoma cell line that produces a monoclonal antibody to human Hageman factor (HF, factor XII) is described. The antibody (P 5–2– 1) consists of mouse IgG2b heavy chains and lambda light chains, selectively neutralizes HF procoagulant activity, and prevents the proteolytic cleavage of HF during contact activation in plasma. When HF is exposed to P 5–2–1 before the absorption of HF to kaolin, HF procoagulant activity is markedly inhibited. In contrast, P 5–2–1 does not interfere with HF activity after the adsorption of HF to kaolin. P 5–2–1 does not inactivate the prekallikrein–activ

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40

Wendling, Alex, Graham Chapman, Nicholas J. Greco, et al. "Proteomic Characterization of Vascular Endothelial Cell-Conditioned Medium Chemo-Attractive to Umbilical Cord Blood Monocytes: Implications for Angiogenesis In Response to Hypoxia." Blood 116, no. 21 (2010): 1487. http://dx.doi.org/10.1182/blood.v116.21.1487.1487.

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Abstract Abstract 1487 Peripheral blood monocytes play a central role in angiogenesis in a variety of physiologic and pathologic processes. To determine whether UCB-derived monocytes respond to factors elicited by injured vascular endothelial cells, we measured UCB-derived monocyte chemotaxis to human umbilical vein endothelial cells (HUVEC)-conditioned media in hypoxic conditions (5% O2). HUVECs in endothelial growth media (EGM) containing HGF, IGF, FGF, heparin, EGF) media were grown until confluent under normoxic conditions. At confluence, HUVEC grown in the EGM media were washed and replac

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41

Vacas, Susana, Vincent Degos, Kevin J. Tracey, and Mervyn Maze. "High-mobility Group Box 1 Protein Initiates Postoperative Cognitive Decline by Engaging Bone Marrow–derived Macrophages." Anesthesiology 120, no. 5 (2014): 1160–67. http://dx.doi.org/10.1097/aln.0000000000000045.

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Abstract Background: Aseptic trauma engages the innate immune response to trigger a neuroinflammatory reaction that results in postoperative cognitive decline. The authors sought to determine whether high-mobility group box 1 protein (HMGB1), an ubiquitous nucleosomal protein, initiates this process through activation and trafficking of circulating bone marrow–derived macrophages to the brain. Methods: The effects of HMGB1 on memory (using trace fear conditioning) were tested in adult C57BL/6J male mice; separate cohorts were tested after bone marrow–derived macrophages were depleted by clodro

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Clayton, R. N., L. C. Bailey, S. D. Abbot, A. Detta, and K. Docherty. "Cyclic adenosine nucleotides and growth hormone-releasing factor increase cytosolic growth hormone messenger RNA levels in cultured rat pituitary cells." Journal of Endocrinology 110, no. 1 (1986): 51–57. http://dx.doi.org/10.1677/joe.0.1100051.

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ABSTRACT The cellular mechanisms involved in GH biosynthesis have been investigated by the measurement of steady-state levels of cytosolic GH messenger RNA (mRNA) in primary cultures of rat pituitary cells using an RNA–complementary DNA (cDNA) hybridization assay. Growth hormone mRNA–cDNA hybridization increased in a linear manner with increasing cytosol concentration. Cellular GH mRNA levels rose by an average of 2·4-fold (range, 1·6–3·3; n = five experiments) after exposure to GH-ieleasing factor (GRF(1–40); 10 nmol/l) for 3 days. Treatment with GRF increased the release of GH into the cultu

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43

Asempa, Tomefa E., Kamilia Abdelraouf та David P. Nicolau. "Metallo-β-lactamase resistance in Enterobacteriaceae is an artefact of currently utilized antimicrobial susceptibility testing methods". Journal of Antimicrobial Chemotherapy 75, № 4 (2020): 997–1005. http://dx.doi.org/10.1093/jac/dkz532.

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Abstract Background MBLs are a major contributor to β-lactam resistance when tested using CAMHB. Despite in vitro resistance, positive outcomes have been reported in MBL-infected patients following carbapenem treatment. The impact of physiological zinc concentrations on this in vitro–in vivo MBL discordance warrants investigation. Objectives To evaluate meropenem in vitro activity against MBL-producing Enterobacteriaceae in zinc-depleted broth (Chelex-CAMHB, EDTA-CAMHB) and assess meropenem efficacy in murine infection models. Methods Neutropenic mice received a meropenem human-simulated regim

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44

Hanna, P. M., D. R. McMillin, M. Pasenkiewicz-Gierula, W. E. Antholine, and B. Reinhammar. "Type 2-depleted fungal laccase." Biochemical Journal 253, no. 2 (1988): 561–68. http://dx.doi.org/10.1042/bj2530561.

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Although copper is quantitatively removed from fungal laccase (Polyporus versicolor) by extended dialysis against high concentrations of cyanide, we have been unable to reconstitute the protein by addition of Cu(I) ions. However, two new methods for reversibly removing the type 2 Cu centre have been developed. The visible absorption at 610 nm, which is attributable to type 1 Cu, is unaffected by the procedure, but the absorbance of the type 3 Cu at 330 nm is decreased by 60 +/- 10%. The decrease is due, at least in part, to partial reduction of the binuclear type 3 centre, although there may b

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45

Ozaki, Katsutoshi, Akiko Meguro, Keiko Hatanaka, et al. "Lack of IL-21 Signal Attenuates Graft-Versus-Leukemia Effect In the Absence of CD8 T-Cells." Blood 116, no. 21 (2010): 3739. http://dx.doi.org/10.1182/blood.v116.21.3739.3739.

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Abstract Abstract 3739 Introduction: IL-21 is a pleiotropic cytokine belongs to a common cytokine receptor g chain family of cytokines. IL-21 is mainly produced by activated CD4+ T cells and acts on T-, B-, NK-cells, and other lineages. IL-21 can drive Th17 differentiation and contributes to the development of autoimmune disease. Previously, we have shown that IL-21R−/− (KO) splenocytes ameliorate GVHD as compared to wild type (WT) splenocytes (BMT 2010), indicating a critical role of IL-21 in GVHD. Bucher et al. reported that IL-21 neutralization resulted in the same results as ours and it di

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46

Zorn, Julia, and Hans Jochem Kolb. "Graft-Versus-Host Tolerance in Dogs Following T-Cell Depleted Bone Marrow Transplantation with Absorbed ATG or CD6-Antibody." Blood 112, no. 11 (2008): 2338. http://dx.doi.org/10.1182/blood.v112.11.2338.2338.

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Abstract Graft-versus-host disease (GvHD) is the major obstacle of allogeneic stem cell transplantation. Depletion of T-cells from the graft reduces the risk of GvHD, but results in a higher risk of leukemia relapse. Adoptive immunotherapy with donor lymphocyte transfusion (DLT) has been shown to control leukemia in patients after T-cell depleted allogeneic stem cell transplantation. However, GvHD may occur, if DLT is given too early after transplantation. In canine models of DLA-identical and DLA-haploidentical bone marrow transplantation, we compared different methods of T-cell depletion (TC

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47

Dodero, Anna, Cristiana Carniti, Anna Raganato, et al. "CD8-Depleted Donor Lymphocyte Infusions Can Improve Both T- and B-Cell Reconstitution after Allogeneic Stem Cell Transplantation from Haploidentical Family Donors." Blood 112, no. 11 (2008): 354. http://dx.doi.org/10.1182/blood.v112.11.354.354.

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Abstract Purpose: Haploidentical hematopoietic stem cell transplantation (haplo-SCT) provides an option for cancer patients lacking a compatible donor. However, the delayed immune reconstitution increases incidence of opportunistic infections and disease relapse. We conducted a phase I-II prospective trial to evaluate the effect of escalating doses of CD8-depleted (Miltenyi Device) donor lymphocyte infusions (DLIs) following RIC haplo-SCT. Here, we present the data on the immune reconstitution. Patients and Methods: Twenty-eight patients (pts) with advanced lymphoproliferative diseases (n=24)

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48

Meyer, Ralf G., Daniela Wehler, Thomas C. Wehler, et al. "Campath-1H-Based Reduced-Intensity Conditioning Followed by Allogeneic Blood Stem-Cell Transplantation and Preemptive CD8-Depleted Donor Lymphocyte Infusions." Blood 104, no. 11 (2004): 5069. http://dx.doi.org/10.1182/blood.v104.11.5069.5069.

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Abstract Recent reports have demonstrated the feasibility of using the anti-CD52 antibody Campath-1H for in vivo T-cell depletion (TCD) in the context of a fludarabine/melphalan-based reduced-intensity conditioning regimen (Kottaridis et al. Blood 2000, 96:2419). Major disadvantage of this protocol is the severe immunosuppression which results in an increased rate of opportunistic infections and disease relapses. Donor lymphocyte infusions (DLI) are frequently used to overcome this limitation. The application of DLI, however, is associated with a profound risk of GvHD. Depletion of CD8+ lympho

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49

Smits, E., P. K. Rose, T. Gordon, and F. J. Richmond. "Organization of single motor units in feline sartorius." Journal of Neurophysiology 72, no. 4 (1994): 1885–96. http://dx.doi.org/10.1152/jn.1994.72.4.1885.

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1. We depleted single motor units in feline sartorius muscles of glycogen by stimulating their motoneurons intracellularly. We mapped the intramuscular distribution of depleted fibers by inspecting histological cross-sections throughout the length of sartorius. 2. We selected ten depleted motor units for detailed study and quantitative analysis. Nine motor units were located in the anterior head of sartorius. One was located in a muscle whose distal half appeared to have been damaged some time before the acute experiment. A single motor unit was located in the medial head of sartorius. 3. Five

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Corradini, Paolo, Anna Raganato, Matteo Carrabba, et al. "Improved Immune Reconstitution Following Pre-Emptive CD8-Depleted Donor Lymphocyte Infusions (DLIs) after Haploidentical Stem Cell Transplantation (SCT) Using a Reduced-Intensity Conditioning (RIC) Regimen." Blood 106, no. 11 (2005): 2908. http://dx.doi.org/10.1182/blood.v106.11.2908.2908.

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Abstract Allogeneic stem cell transplantation (SCT) from an haploidentical family donor has been reported as a viable option in acute leukemias when matched donors are unavailable. However, the extensive T-cell depletion (TCD), required to prevent graft-versus-host disease (GVHD), is associated to delayed immune recovery and high transplant-related mortality. In an ongoing phase I–II trial for patients (pts) with advanced hematological malignancies, we combined a RIC regimen, including thiotepa (10 mg/kg), fludarabine (120 mg/ms), cyclophosphamide (60 mg/kg) and TBI (2 Gy), with pre-emptive ad

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