Relevant bibliographies by topics / 1-diphenyl-2-picrylhydrazyl radical (DPPH) assay

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Academic literature on the topic '1-diphenyl-2-picrylhydrazyl radical (DPPH) assay'

Author: Grafiati
Published: 4 June 2025
Last updated: 15 July 2025

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Journal articles on the topic "1-diphenyl-2-picrylhydrazyl radical (DPPH) assay"

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Luo, Aoxue, Ping Peng, Wen Feii, Linying Yang, and Yijun Fan. "Isolation and antioxidant activity (in vitro and in vivo) of the flavonoid from Tartarian-buckwheat." Journal of Scientific and Innovative Research 3, no. 2 (2014): 168–72. http://dx.doi.org/10.31254/jsir.2014.3210.

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The total flavonoid was isolated from tartarian-buckwheat through reflux extraction. In order to explore the antioxidant activities, in the present study, our study aimed to examine the antioxidant activity of the total flavonoid using different assays including: reducing power, 2,2- diphenyl 1-picrylhydrazyl (DPPH) radical scavenging assay, ABTS radical scavenging assay and hydroxyl radical scavenging activity. The results exhibited that the total flavonoid has significant reductive ability and radicals scavenging effects. In vivo antioxidant activities assays, the flavonoid was found to incr
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Tantry, Mudasir A., and Aziz A. Rahman. "Amplexicine, an Antioxidant Flavan-3-ol from Polygonum amplexicaule." Natural Product Communications 6, no. 11 (2011): 1934578X1100601. http://dx.doi.org/10.1177/1934578x1100601111.

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Bioassay guided fractionation of an ethanolic extract of Polygonum amplexicaule D. Don led to the isolation of amplexicine, a new flavan-3-ol (1), along with khellactone (2). The structure of the isolates was established by UV, IR, HRESI/MS and NMR, including 1D and 2D experiments. Compound 1 exhibited considerable antioxidant activity in a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay.
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Salem, Hesham. "SPECTROPHOTOMETRIC DETERMINATION OF SOME PHARMACEUTICAL COMPOUNDS USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL." Scientia Pharmaceutica 68, no. 4 (2000): 403–19. http://dx.doi.org/10.3797/scipharm.aut-00-37.

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A simple, rapid and sensitive spectrophotometric procedure for the assay of some drugs has been developed. The method is based on the reaction of the studied drugs with 2,2-diphenyl-1-picrylhydrazyl (DPPH). The latter is employed to abstract a hydrogen atom from the drugs thereby promoting a process of radical coupling. This results in a reduction of the violet color of DPPH with the formation of the yellow colored 2,2-diphenyl-1-picrylhydrazine (DPPH2). The decrease in the intensity of the violet color is used to measure the concentration of the drugs. All measurements are made at λ= 520 nm o
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Wang, Xiu, Yupei Zhang, Nan Wu, Jingya Cao, Yanduo Tao, and Ruitao Yu. "A Method to Separate Two Main Antioxidants from Lepidium latifolium L. Extracts Using Online Medium Pressure Chromatography Tower and Two-Dimensional Inversion/Hydrophobic Interaction Chromatography Based on Online HPLC-DPPH Assay." Separations 8, no. 12 (2021): 238. http://dx.doi.org/10.3390/separations8120238.

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Free radicals, including 1,1-diphenyl-2-picrylhydrazyl, mediate oxidative stress to cause many chronic diseases (including cardiovascular diseases, diabetes and cancer). The extract of traditional Tibetan medicine Lepidium latifolium L. (L. latifolium) was reported to have free radical inhibition ability. Therefore, a system method was established to separate the ethanol extract of L. latifolium to prepare two main antioxidant compounds. First of all, silica gel and a medium-pressure liquid chromatography tower were used for pre-treatment of the ethanol extract of L. latifolium to obtain the m
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Yulyana, A., E. Mugiyanto, D. G. Guntara, et al. "Radical scavenging activity of ethanolic extract of green and red leaves of Cantigi (Vaccinium varingiaefolium Bl. Miq.)." Food Research 9, no. 2 (2025): 13–19. https://doi.org/10.26656/fr.2017.9(2).607.

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Indonesia's Cantigi (Vaccinium varingiaefoilum Bl. Miq.) is a member of the blueberry family that grows abundantly around volcanoes. It has two distinct leaf colors, red color leave (RCL) and green color leave (GCL), which contain secondary metabolites including anthocyanins. This study aimed to characterize the antioxidant properties of Cantigi leaf using various methods. DPPH (2,2’-diphenyl-1-picrylhydrazyl), BHA (butylhydroxyanisole), and BHT (butyl hydroxytoluene) were used to check the antioxidant activity of 96% ethanolic Cantigi extract. The phytochemical screening showed positive resul
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Manimaran, Manickavelu, and Krishnan Kannabiran. "Marine Streptomyces Sp. VITMK1 Derived Pyrrolo [1, 2-A] Pyrazine-1, 4-Dione, Hexahydro-3-(2-Methylpropyl) and Its Free Radical Scavenging Activity." Open Bioactive Compounds Journal 5, no. 1 (2017): 23–30. http://dx.doi.org/10.2174/1874847301705010023.

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Background: Free radical generation has been proved to be responsible for various cellular diseases. It is necessary to combat free radicals using antioxidants derived from natural sources. Objective: The objective of this study is to evaluate the antioxidant activity of the diketopiperazine compound extracted from Streptomyces sp. VITMK1 isolated from mangrove sediment soil collected from Pichavaram, Tamil Nadu, India. Methods: The antioxidant potential of pyrrolo [1, 2-A] pyrazine-1, 4-dione, hexahydro-3-(2-methylpropyl) (diketopiperazine) extracted from Streptomyces sp. VITMK1 was studied u
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Fitriana, Wiwit Denny, Taslim Ersam, Kuniyoshi Shimizu, and Sri Fatmawati. "Antioxidant Activity of Moringa oleifera Extracts." Indonesian Journal of Chemistry 16, no. 3 (2018): 297. http://dx.doi.org/10.22146/ijc.21145.

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Moringa oleifera have been evaluated for its antioxidant activity. M. oleifera leaves were extracted with methanol, ethyl acetate, dichloromethane and n-hexane. The antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay and an improved 2,2’-azino-bis-[3-ethylbenzothiazoline sulphonate] (ABTS) radical cation decolorization assay in vitro. Trolox was used as standard with IC50 5.89 μg/mL in DPPH assay and 3.06 μg/mL in ABTS assay. The methanol extract showed the highest free radical scavenging activity with IC50 value of 49.
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Jassal, Prabhjot Singh, and Gagandeep Kaur. "COMPARATIVE ANALYSIS OF ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL CONTENTS IN ETHANOLIC LEAF EXTRACTS OF IN VITRO AND FIELD GROWN WITHANIA SOMNIFERA." Asian Journal of Pharmaceutical and Clinical Research 9, no. 5 (2016): 239. http://dx.doi.org/10.22159/ajpcr.2016.v9i5.13370.

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ABSTRACTObjective: The present study was planned to compare antioxidant activity in vitro and field grown Withania somnifera was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) assays. Medicinal plants are a major source of phytochemicals used for the treatments ofhuman diseases. W. somnifera has anti-inflammatory, antioxidant, and antimicrobial properties.Methods: Antioxidant activity and phytochemical contents in W. somnifera were determined spectrophotometrically.Results: The results of antioxidant activity of field grown ethanolic leaf extract of W. somnifera show
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Basnet, Purusotam, Tetsuya Matsuno, and Richard Neidlein. "Potent Free Radical Scavenging Activity of Propol Isolated from Brazilian Propolis." Zeitschrift für Naturforschung C 52, no. 11-12 (1997): 828–33. http://dx.doi.org/10.1515/znc-1997-11-1217.

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Abstract We evaluated free radical scavenging activity of the water, methanol and chloroform ex­tracts of propolis in 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and xanthine-xanthine oxidase (XOD) generated superoxide anion assay systems. The free radical scavenging activity guided fractionation and chemical analysis led to the isolation of a new compound, propol {3-[4-hydroxy-3-(3-oxo-but-1-enyl)-phenyl]-acrylic acid) from the water extract, which was more potent than most common antioxidants such as vitamin C and vitamin E (α-tocopherol) in these assay systems.
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Handayani, Virsa, Aktsar Roskiana Ahmad, Nur Rezky Khairun Nisaa, La Hamidu, and Ahmad Najib. "Postprandial Bioassay and Radical Scavenging on n-Hexane Fraction of Cordia myxa L. Leaf." Jurnal Fitofarmaka Indonesia 9, no. 3 (2022): 13–18. http://dx.doi.org/10.33096/jffi.v9i3.917.

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Cordia myxa L. is empirically used as an antidiabetic medication. The aim of this study is to determine the in-vivo effect of Cordia myxa L. n-hexane fraction on reducing blood sugar levels and their relationship with the anti-free-radical activity assay using DPPH (1,1-diphenyl-2-Picrylhydrazyl). The n-hexane fraction of Cordia myxa L. was used as a sample in the post-prandial assay which was analyzed using the ANOVA statistical test followed by Post-Hoc test. The results showed that there were significant in the extract using a 500 mg dose. In the anti-free-radical test using DPPH (1,1-diphe
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Book chapters on the topic "1-diphenyl-2-picrylhydrazyl radical (DPPH) assay"

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Londonkar, Ramesh, and Maithilee Kesralikar. "In Vitro Anticancer Screening of Methanolic Extract of Stachytarpheta Mutabilis." In Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022). Atlantis Press International BV, 2022. http://dx.doi.org/10.2991/978-94-6463-020-6_19.

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AbstractIn therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. The species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, and saponins, among other phytoconstituents. One fungus, Aspergillus niger, Staphylococcus aureus, and Bacillus subtilis, as well as four-gram (+) pathogens, including Staphylococcus aureus and Bacillus subtilis, and two gram (-) pathogens, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae, were disc diffusion tested for antibacterial activity. The nitric oxide scavenging assay was used to test for anti-inflammatory efficacy in vitro. DPPH, ABTS, and total antioxidant tests were used to evaluate antioxidant activity. Analyses such as TLC, GC-MS, and were performed as per common procedure. A nitric oxide scavenging experiment was utilised to determine the anti-inflammatory properties of the drug in vitro. Tests for antioxidant activity were conducted using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and total antioxidant tests. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) analysis has been used to do anticancer screening. Extracts from Stachytarpheta mutabilis were found to include flavonoids, tannins and phenolic compounds as well as terpenoids and steroids in their phytochemical analysis. Squalene, octadecanal, and butan-1-one were found in the GC-MS study as were thirty other bioactive chemicals such as 4-isopropylcinnamic acid and octadecanoic acid. The methanolic fractions of plant extracts were shown to be effective against all bacteria. Anti-inflammatory and antioxidant experiments performed in the lab revealed the compounds’ potent inhibition activity. Analysis of the GC-MS data revealed the existence of thirty distinct components. MTT assay on the extract revealed anticancer activity. The data shows that the methanolic extract has a significant properties. Stachytarpheta mutabilis has therapeutic value and other pharmaceutical qualities may be assessed as a result of these results, which support the traditional usage of folk medicine as well.
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Florin Danet, Andrei. "Recent Advances in Antioxidant Capacity Assays." In Antioxidants - Benefits, Sources, Mechanisms of Action. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96654.

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This work presents a survey of the important antioxidant capacity/activity assays applied for a diversity of samples including plant extracts, foods, biological material, etc. The published materials are critically discussed, emphasizing the recent findings in the field. New and emergent antioxidant capacity assays, such as nanoparticles-based assay, are also presented. The discussion includes chemical-based methods as well as biochemical and cellular assays. Chemical methods detailed are radical/ROS-based scavenging assays (the trolox equivalent antioxidant capacity (TEAC/ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC) assays, chemiluminescence methods, total radical-trapping antioxidant parameter (TRAP), total oxy radical scavenging capacity (TOSC), and β-carotene bleaching assays), non-radical redox potential-based assays (ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), nanoparticle-based methods and electrochemical methods), metal chelation capacity and total phenolic content tests. The biochemical-based assays and in vivo assays discussed include the oxidation of low density lipoprotein (LDL), the thiobarbituric acid reactive substances (TBARS) and the cellular antioxidant activity (CAA) assays. While a direct link between the antioxidant capacity and health benefits is still a matter of debate, the antioxidant testing methodologies presented in this chapter remain valuable for the high efficiency and cost-effective evaluation of antioxidants, from compound discovery to quality control.
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Kusmiati, Kusmiati, Fifi Afiati, Chrisna Widhiani, Alpinna Aditia, Destia D. Elviani, and Atit Kanti. "The Potential of Lutein Extract of Tagetes erecta L. Flower as an Antioxidant and Enhancing Phagocytic Activity of Macrophage Cells." In Innovation in the Food Sector Through the Valorization of Food and Agro-Food By-Products. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.95965.

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Marigold flower (Tagetes erecta L.) produces lutein compounds which present biological activities such as antioxidant, antiinflammatory, antimutagenicity, and immunomodulatory effects. The study was to investigate the antioxidant activity of the lutein of T. erecta L. and the effect of lutein on the activity and phagocytic capacity of macrophage cells. The antioxidant screening was carried out using diphenyl picrylhydrazyl (DPPH), 2,2′-and-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging assay with serial concentrations and ferric-reducing antioxidant power (FRAP) method. For the observation of activity and phagocytic capacity of peritoneal macrophages, twenty-eight mice were used and divided into seven groups each comprising four replicates, i.e., Group (I) normal controls, mice were untreated (II) a negative control, mice were induced by Staphylococcus aureus (III) positive control, mice were induced by S. aureus and treatment of meniran extract (Phyllanthus niruri). The treatment group (IV-VII) mice were induced by S. aureus and treated crude lutein, respectively: 0.15 mg, 0.30 mg, 0.60 mg, and 0.90 mg. 20 g−1 of body weight. The lutein extracted from T. erecta shows an antioxidant activity against DPPH radical with an IC50 value of 53.58 μg.ml−1, while the antioxidant activity against ABTS has an IC50 value of 72.91 μg.ml−1. The antioxidant activity test results by the FRAP method at each lutein concentrations of 10, 25, 50, and 75 ppm were obtained respectively of 33, 88, 185.5, and 288.5 μmol Fe2+/g extract. The data were analyzed using one-way ANOVA and Duncan’s multiple range test (DMRT) after. The phagocytic activity was 45.5%; 54.75%; 57.50% and 67.0%, respectively, while the phagocytic capacity values ​​were 355; 519; 611 and 767 S. aureus bacterial cells per 50 macrophage cells. The lutein from marigolds (T. erecta L.) is capable of scavenging free radicals and reducing oxidants. Lutein can increase the activity and capacity of phagocytic of peritoneum macrophage cells in mice.
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Conference papers on the topic "1-diphenyl-2-picrylhydrazyl radical (DPPH) assay"

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"Determination of Antioxidant Property, Total Phenolics Content, and α-Glucosidase Inhibitory Activity of Different Solvent Extracts of Defatted and Non-Defatted Peanut Skins". У 4th International Conference on Biological & Health Sciences (CIC-BIOHS’2022). Cihan University, 2022. http://dx.doi.org/10.24086/biohs2022/paper.781.

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Peanut skins are by-product with little economic value. The effect of different solvents, on the total phenolic content (TPC), antioxidant property and in vitro antidiabetic activity of defatted and non-defatted peanut skins was determined. TPC was estimated using Folin-Ciocalteau reagent. Antioxidant property was evaluated using ferric reducing antioxidant power (FRAP) and 2, 2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging capacity methods, α-glucosidase inhibition was evaluated using 4- nitrophenyl α-D-glucopyranoside (pNPG) method. Peanut skin of the defatted extracted with ethanol h
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Berechet, Mariana Daniela, Demetra Simion, Maria Stanca, et al. "Antibacterial and antioxidant activities of lemon balm (Melissa officinalis L.) essential oil." In The 8th International Conference on Advanced Materials and Systems. INCDTP - Leather and Footwear Research Institute (ICPI), Bucharest, Romania, 2020. http://dx.doi.org/10.24264/icams-2020.ii.2.

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Lemon balm (Melissa officinalis L.) belongs to the Lamiaceae family. Essential oil extracted from the aerial parts of lemon balm has been investigated for the protection of fruits during storage, as insecticidal, as well as in medicine, due to its bioactive properties. In this paper, the composition and identification of components from Melissa officinalis L. essential oil were determined by gas chromatography coupled with mass spectrophotometry (GC/MS) analysis. Total phenol content (TPC) and the scavenging activity towards 2,2-diphenyl-1-picrylhydrazyl (DPPH·) and 2,2′-azino-bis (3-ethylbenz
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