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Relevant bibliographies by topics / 13C isotope labeling

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Academic literature on the topic '13C isotope labeling'

Author: Grafiati

Published: 5 June 2025

Last updated: 1 August 2025

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Journal articles on the topic "13C isotope labeling"

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Sivashankari, Ramamoorthi M., Yuki Miyahara, and Takeharu Tsuge. "Poly(3-Hydroxybutyrate) Biosynthesis from [U-13C6]D-Glucose by Ralstonia eutropha NCIMB 11599 and Recombinant Escherichia coli." Microbiology Research 14, no. 4 (2023): 1894–906. http://dx.doi.org/10.3390/microbiolres14040129.

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The use of stable isotope-labeled polymers in in situ biodegradation tests provides detailed information on the degradation process. As isotope-labeled raw chemicals are generally expensive, it is desirable to prepare polymer samples with high production yields and high isotope-labeling ratios. The biodegradable plastic poly[(R)-3-hydroxybutyrate)] (P(3HB)) is produced by microorganisms. In this study, to produce carbon 13 (13C)-labeled P(3HB) from [U-13C6]D-glucose (13C-glucose), the culture conditions needed for high production yields and high 13C-labeling ratios were investigated using Rals

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Ali, Rustam, Lindsay D. Clark, Jacob A. Zahm, et al. "Improved strategy for isoleucine 1H/13C methyl labeling in Pichia pastoris." Journal of Biomolecular NMR 73, no. 12 (2019): 687–97. http://dx.doi.org/10.1007/s10858-019-00281-1.

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Abstract Site specific methyl labeling combined with methyl TROSY offers a powerful NMR approach to study structure and dynamics of proteins and protein complexes of high molecular weight. Robust and cost-effective methods have been developed for site specific protein 1H/13C methyl labeling in an otherwise deuterated background in bacteria. However, bacterial systems are not suitable for expression and isotope labeling of many eukaryotic and membrane proteins. The yeast Pichia pastoris (P. pastoris) is a commonly used host for expression of eukaryotic proteins, and site-specific methyl labelin

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Miyatake, Tetsuro, Barbara J. MacGregor, and Henricus T. S. Boschker. "Linking Microbial Community Function to Phylogeny of Sulfate-Reducing Deltaproteobacteria in Marine Sediments by Combining Stable Isotope Probing with Magnetic-Bead Capture Hybridization of 16S rRNA." Applied and Environmental Microbiology 75, no. 15 (2009): 4927–35. http://dx.doi.org/10.1128/aem.00652-09.

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ABSTRACT We further developed the stable isotope probing, magnetic-bead capture method to make it applicable for linking microbial community function to phylogeny at the class and family levels. The main improvements were a substantial decrease in the protocol blank and an approximately 10-fold increase in the detection limit by using a micro-elemental analyzer coupled to isotope ratio mass spectrometry to determine 13C labeling of isolated 16S rRNA. We demonstrated the method by studying substrate utilization by Desulfobacteraceae, a dominant group of complete oxidizing sulfate-reducing Delta

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Fahey, Timothy J., Kayla R. Jacobs, and Ruth E. Sherman. "Fine root turnover in sugar maple estimated by 13C isotope labeling." Canadian Journal of Forest Research 42, no. 10 (2012): 1792–95. http://dx.doi.org/10.1139/x2012-128.

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We evaluated variation in root turnover across five root orders in sugar maple ( Acer saccharum Marsh.) saplings growing in a northern hardwood forest in central New York, USA. We used a stable isotope approach in which root systems were labeled with 13C and root structural C sequentially sampled for 13C enrichment. Turnover of first- and second-order roots was apparently rapid with only about 5% of the 13C retained in living roots after two growing seasons. Although third- to fifth-order roots appeared to persist longer, differences among root orders were not statistically significant, probab

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Bagga, Puneet, Kevin L. Behar, Graeme F. Mason, Henk M. De Feyter, Douglas L. Rothman, and Anant B. Patel. "Characterization of Cerebral Glutamine Uptake from Blood in the Mouse Brain: Implications for Metabolic Modeling of 13C NMR Data." Journal of Cerebral Blood Flow & Metabolism 34, no. 10 (2014): 1666–72. http://dx.doi.org/10.1038/jcbfm.2014.129.

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13C Nuclear Magnetic Resonance (NMR) studies of rodent and human brain using [1-13C]/[1,6-13C2]glucose as labeled substrate have consistently found a lower enrichment (~25% to 30%) of glutamine-C4 compared with glutamate-C4 at isotopic steady state. The source of this isotope dilution has not been established experimentally but may potentially arise either from blood/brain exchange of glutamine or from metabolism of unlabeled substrates in astrocytes, where glutamine synthesis occurs. In this study, the contribution of the former was evaluated ex vivo using 1H-[13C]-NMR spectroscopy together w

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Jin, S. J., and K. Y. Tserng. "Biogenesis of dicarboxylic acids in rat liver homogenate studied by 13C labeling." American Journal of Physiology-Endocrinology and Metabolism 261, no. 6 (1991): E719—E724. http://dx.doi.org/10.1152/ajpendo.1991.261.6.e719.

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The aim of this investigation is to assess whether long-chain fatty acids can be a substrate for omega-oxidation and the subsequent beta-oxidation to produce medium-chain dicarboxylic acids normally found in urine. Isolated rat liver 10,000 g supernatant and pellet fractions were used as the source of enzymes. The metabolism of palmitate was studied using [1,2,3,4-13C4]hexadecanoic acid as tracer. Selected ion monitoring mass spectrometry was utilized for the determination of isotope enrichments in precursor and products. Palmitate was found to be a good substrate for omega-oxidation; the rate

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Kim, Hee-Youn, Eun-Mi Hong, and Weon-Tae Lee. "Cost-effective isotope labeling technique developed for15N/13C-labeled proteins." Journal of the Korean Magnetic Resonance Society 15, no. 2 (2011): 115–27. http://dx.doi.org/10.6564/jkmrs.2011.15.2.115.

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Robins, Richard, Katarzyna Romek, Mathilde Grand, et al. "Difficulties in Differentiating Natural from Synthetic Alkaloids by Isotope Ratio Monitoring using 13C Nuclear Magnetic Resonance Spectrometry." Planta Medica 84, no. 12/13 (2018): 935–40. http://dx.doi.org/10.1055/a-0601-7157.

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AbstractWithin the food and pharmaceutical industries, there is an increasing legislative requirement for the accurate labeling of the productʼs origin. A key feature of this is to indicate whether the product is of natural or synthetic origin. With reference to this context, we have investigated three alkaloids commonly exploited for human use: nicotine, atropine, and caffeine. We have measured by 13C nuclear magnetic resonance spectrometry the position-specific distribution of 13C at natural abundance within several samples of each of these target molecules. This technique is well suited to

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Novotny, Jessie Lanterman, and Karen Goodell. "Utility of carbon and nitrogen stable isotopes for inferring wild bee (Hymenoptera: Apoidea) use of adjacent foraging habitats." PLOS ONE 17, no. 7 (2022): e0271095. http://dx.doi.org/10.1371/journal.pone.0271095.

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Isotope analysis has proven useful for understanding diets of animals that are difficult to track for extended periods. Bees are small yet highly mobile and often forage from multiple habitats. However, current methods of assessing diet are limited in scope. Efficient methods of tracking bee diets that integrate across life stages, distinguish habitat use, and are sensitive to taxonomic differences will inform conservation strategies. We evaluated the utility of stable isotope analysis for estimating contributions of adjacent habitats to bees’ diets. We also investigated taxonomic variation in

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Takeda, Mitsuhiro, Yohei Miyanoiri, Tsutomu Terauchi, and Masatsune Kainosho. "Conformational features and ionization states of Lys side chains in a protein studied using the stereo-array isotope labeling (SAIL) method." Magnetic Resonance 2, no. 1 (2021): 223–37. http://dx.doi.org/10.5194/mr-2-223-2021.

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Abstract. Although both the hydrophobic aliphatic chain and hydrophilic ζ-amino group of the Lys side chain presumably contribute to the structures and functions of proteins, the dual nature of the Lys residue has not been fully investigated using NMR spectroscopy, due to the lack of appropriate methods to acquire comprehensive information on its long consecutive methylene chain. We describe herein a robust strategy to address the current situation, using various isotope-aided NMR technologies. The feasibility of our approach is demonstrated for the Δ+PHS/V66K variant of staphylococcal nucleas

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Dissertations / Theses on the topic "13C isotope labeling"

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Ghirardo, Andrea [Verfasser]. "Studies of plant terpenoid biosynthesis using 13C stable isotope labeling techniques (KIT Scientific Reports ; 7583) / Andrea Ghirardo." Karlsruhe : KIT Scientific Publishing, 2011. http://d-nb.info/1185581405/34.

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Roy, Chowdhury Taniya. "Tracking Carbon Flow during Methane Oxidation into Methanotrophs using 13C-PLFA Labeling in Pulsing Freshwater Wetlands." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1339084813.

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Haynes, Christopher Allen. "Development of an assay for fatty acyl-CoAs using liquid chromatography-electrospray ionization-tandem mass spectrometry and its application to the stable isotope labeling and quantitation of sphingolipid metabolism." Diss., Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/37171.

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Fatty acyl-Coenzyme As are metabolites of lipid anabolism and catabolism. A method was developed for their quantitation in extracts of cultured mammalian cells using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Palmitoyl-CoA (C16:0-CoA) is utilized for de novo sphingolipid biosynthesis catalyzed by serine palmitoyltransferase (SPT), which condenses palmitoyl-CoA and serine to form 3-ketosphinganine. After reduction to form sphinganine (Sa), dihydroceramide synthase (CerS) can N-acylate the Sa using a second fatty acyl-CoA molecule, forming dihydrocera

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Zhou, Wenxuan. "Stoichiometry and Crystal Structure of Poly (Lactic Acid) (PLA) Stereocomplex (SC) in Cold-crystallization and Solution-grown Crystals as Studied by Solid-state NMR and 13C Isotope Labeling." University of Akron / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=akron1522239647112751.

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Campanholi, Diana Ruffato Resende. "Oxidação da galactose utilizando 13C em crianças saudáveis e galactosêmicas." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/17/17144/tde-22042014-101217/.

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A Galactosemia é um erro inato do metabolismo da galactose que ocorre em consequência da deficiência de uma das três principais enzimas envolvidas em seu metabolismo. O tratamento da doença se faz por meio de intervenção dietética. Com a necessidade de melhorar nosso conhecimento acerca do metabolismo da galactose, isótopos estáveis estão sendo usados para mostrar o perfil de oxidação da mesma. O parâmetro bioquímico claramente correlacionado com os resultados clínicos e com o genótipo da GALT é o da oxidação da galactose em dióxido de carbono. A capacidade de determinar a oxidação com adminis

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Kheir, Beik Louay. "Dynamics of soil organic matter amino acids : a carbon isotope approach." Thesis, Aix-Marseille, 2017. http://www.theses.fr/2017AIXM0098.

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Cette thèse aborde un point clé du couplage entre ces cycles: la dynamique des molécules azotées (AAs) des matières organiques du sol (MOS). Par des expériences d'incubation, nous avons estimé que les flux de biosynthèse des AAs par les micro-organismes du sol lors du processus de décomposition sont de l'ordre de 25% de la biomasse nouvellement formée. Le profil des AAs individuels biosynthétisés de novo est plus dépendant du type de sol que de la nature du substrat. Dans chaque sol, il est très similaire à celui des AAs des MOS. La biodégradation de matériaux végétaux marqués en 13C a révélé

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Olsson, Ulrika. "Structural Studies of O-antigen polysaccharides, Synthesis of 13C-labelled Oligosaccharides and Conformational Analysis thereof, using NMR Spectroscopy." Doctoral thesis, Stockholm University, Department of Organic Chemistry, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-7283.

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<p>In order to understand biological processes, to treat and diagnose diseases, find appropriate vaccines and to prevent the outbreak of epidemics, it is essential to obtain more knowledge about carbohydrate structures. This thesis deals with structure and conformation of carbohydrates, analysed by NMR spectroscopy and MD simulations.In the first two papers, the structures of O-antigen polysaccharides (PS) from two different <i>E. coli</i> bacteria were determined using NMR spectroscopy. The O-antigenic PS from <i>E. coli</i> O152 (paper I) consists of branched pentasaccharide repeating units,

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Åberg, Ola. "Design and Synthesis of 11C-Labelled Compound Libraries for the Molecular Imaging of EGFr, VEGFr-2, AT1 and AT2 Receptors : Transition-Metal Mediated Carbonylations Using [11C]Carbon Monoxide." Doctoral thesis, Uppsala universitet, Institutionen för biokemi och organisk kemi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-98599.

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This work deals with radiochemistry and new approaches to develop novel PET tracers labelled with the radionuclide 11C. Two methods for the synthesis of 11C-labelled acrylamides have been explored. First, [1-11C]-acrylic acid was obtained from a palladium(0)-mediated 11C-carboxylation of acetylene with [11C]carbon monoxide; this could be converted to the corresponding acyl chloride and then combined with benzylamine to form N-benzyl[carbonyl-11C]acrylamide. In the second method, the palladium(0)-mediated carbonylation of vinyl halides with [11C]carbon monoxide was explored. This latter method,

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Eriksson, Jonas. "Synthesis of 11C-labelled Alkyl Iodides : Using Non-thermal Plasma and Palladium-mediated Carbonylation Methods." Doctoral thesis, Uppsala universitet, Avdelningen för organisk kemi, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7171.

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Compounds labelled with 11C (β+, t1/2 = 20.4 min) are used in positron emission tomography (PET), which is a quantitative non-invasive molecular imaging technique. It utilizes computerized reconstruction methods to produce time-resolved images of the radioactivity distribution in living subjects. The feasibility of preparing [11C]methyl iodide from [11C]methane and iodine via a single pass through a non-thermal plasma reactor was explored. [11C]Methyl iodide with a specific radioactivity of 412 ± 32 GBq/µmol was obtained in 13 ± 3% decay-corrected radiochemical yield within 6 min via catalytic

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Nugue, Guillaume. "Développement de méthodes systémiques pour l'amélioration de la connaissance et du traitement des gliomes." Thesis, Grenoble, 2014. http://www.theses.fr/2014GRENS012/document.

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Es gliomes sont des tumeurs cérébrales associées à une mortalité élevée. Le glioblastome multiforme (GBM) est la forme la plus fréquente des tumeurs cérébrales primaires. Malgré une prise en charge thérapeutique optimale constituée d'une chirurgie, d'une radiochimiothérapie concomitante et d'une chimiothérapie adjuvante, la survie médiane est de 15 mois. Ceci s'explique surtout par le potentiel infiltratif de ces tumeurs. Il est donc difficile de réaliser une exérèse chirurgicale totale, ce qui entraine une récidive quasi-systématique avec l'apparition de chimiorésistance. Ces phénomènes de ré

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Book chapters on the topic "13C isotope labeling"

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Motori, Elisa, and Patrick Giavalisco. "13C Isotope Labeling and Mass Spectrometric Isotope Enrichment Analysis in Acute Brain Slices." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3247-5_14.

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Feng, Xueyang, Wei-Qin Zhuang, Peter Colletti, and Yinjie J. Tang. "Metabolic Pathway Determination and Flux Analysis in Nonmodel Microorganisms Through 13C-Isotope Labeling." In Microbial Systems Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-827-6_11.

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Zhong, Qi-ding, Guo-hui Li, Dong-dong Zhao, Dao-bing Wang, Shi-gang Shen, and Zheng-he Xiong. "Low Labeling 13C Metabolic Flux Analysis of Saccharomyces cerevisiae Using Gas Chromatography–Combustion–Isotope Ratio Mass Spectrometry." In Lecture Notes in Electrical Engineering. Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-46318-5_45.

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Boschker, H. T. S. "Section 8 update: Linking microbial community structure and functioning: stable isotope (13C) labeling in combination with PLFA analysis." In Molecular Microbial Ecology Manual. Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-2177-0_807.

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Nogués, Salvador. "Relationship Between Photosynthesis and Respiration in Leaves Using 13C/12C Isotope Labelling." In Advanced Topics in Science and Technology in China. Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-32034-7_63.

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Stevenel, Pierre, E. Frossard, S. Abiven, I. M. Rao, F. Tamburini, and A. Oberson. "Using a Tri-Isotope (13C, 15N, 33P) Labelling Method to Quantify Rhizodeposition." In Methods in Rhizosphere Biology Research. Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5767-1_10.

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Saunders, Eleanor C., David P. de Souza, Jennifer M. Chambers, Milica Ng, James Pyke, and Malcolm J. McConville. "Use of 13C Stable Isotope Labelling for Pathway and Metabolic Flux Analysis in Leishmania Parasites." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1438-8_18.

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Chandra, Rohitash, Mengjie Zhang, and Lifeng Peng. "Application of Cooperative Convolution Optimization for 13C Metabolic Flux Analysis: Simulation of Isotopic Labeling Patterns Based on Tandem Mass Spectrometry Measurements." In Lecture Notes in Computer Science. Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-34859-4_18.

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Priego-Capote, Feliciano, Maria Ramírez-Boo, Denis Hochstrasser, and Jean-Charles Sanchez. "Qualitative and Quantitative Analysis of Glycated Proteins in Human Plasma by Glucose Isotopic Labeling with 13C6-Reducing Sugars." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-068-3_14.

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Cotrufo, M. Francesca, and Yamina Pressler. "Heavy Isotope Enrichments." In A Primer on Stable Isotopes in Ecology. Oxford University PressOxford, 2023. http://dx.doi.org/10.1093/oso/9780198854494.003.0005.

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Abstract “Heavy Isotope Enrichments” reviews concepts for developing and interpreting heavy isotope labeling studies in ecology. The chapter compares continuous- and pulse-labeling approaches for the 13C labeling of plant material. Methods for the application of isotope-labeled substrates and their use in stable isotope probing of biological markers are presented. Calculations of the heavy isotope excess and the quantification of the recovery of heavy isotope additions in isotope tracing studies are provided. Principles and equations for the application of the isotope dilution method are illus

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