Academic literature on the topic '14C pulse labeling'

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Journal articles on the topic "14C pulse labeling"

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Drechsler-Thielmann, Beate, Dieter Dörnemann, and Horst Senger. "Labeling and Isolation of Hemes from Scenedesmus." Zeitschrift für Naturforschung C 47, no. 1-2 (1992): 33–40. http://dx.doi.org/10.1515/znc-1992-1-207.

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Abstract Protoheme and small quantities of heme a were isolated and purified from the wild type and the developmental mutant, C-2 A′, of Scenedesmus obliquus. In fast growing synchronized WT cells and greening cells of the mutant protoheme could be labeled with [1- 14C]glutamate and [2-14C]glycine. The labeling of heme a was ambiguous and of minor quantity. During rapid chlorophyll biosynthesis the turnover of protoheme was determined by a pulse-chase experiment to have a half-life of 2 h. At the current stage of investigations the question, whether [2-14C]glycine is incorporated into protohem
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van der Meer, Marcel T. J., Stefan Schouten, Mary M. Bateson, et al. "Diel Variations in Carbon Metabolism by Green Nonsulfur-Like Bacteria in Alkaline Siliceous Hot Spring Microbial Mats from Yellowstone National Park." Applied and Environmental Microbiology 71, no. 7 (2005): 3978–86. http://dx.doi.org/10.1128/aem.71.7.3978-3986.2005.

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ABSTRACT Green nonsulfur-like bacteria (GNSLB) in hot spring microbial mats are thought to be mainly photoheterotrophic, using cyanobacterial metabolites as carbon sources. However, the stable carbon isotopic composition of typical Chloroflexus and Roseiflexus lipids suggests photoautotrophic metabolism of GNSLB. One possible explanation for this apparent discrepancy might be that GNSLB fix inorganic carbon only during certain times of the day. In order to study temporal variability in carbon metabolism by GNSLB, labeling experiments with [13C]bicarbonate, [14C]bicarbonate, and [13C]acetate we
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Xu, J. G., and N. G. Juma. "Carbon kinetics in a Black Chernozem with roots in situ." Canadian Journal of Soil Science 75, no. 3 (1995): 299–305. http://dx.doi.org/10.4141/cjss95-043.

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The rates of decomposition of roots and root-derived materials are needed to assess the contribution of these materials to sequestration of organic carbon in soil. The objective of this study was to examine the kinetics of different forms of C in a Black Chernozem, with roots in situ under two barley cultivars, using 14C pulse-labeling and incubation methods. Plants were pulse-labeled (1 d) with 14CO2 25 d after emergence. Shoots were excised, and undisturbed soil cores containing the roots of a single plant were incubated at 20 °C for 80 d. The experiment involved two barley cultivars, with s
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Cruz, Nancy F., Keiji Adachi, and Gerald A. Dienel. "Rapid Efflux of Lactate from Cerebral Cortex during K+-Induced Spreading Cortical Depression." Journal of Cerebral Blood Flow & Metabolism 19, no. 4 (1999): 380–92. http://dx.doi.org/10.1097/00004647-199904000-00004.

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Rapid transport of lactate from activated brain regions to blood, perhaps reflecting enhanced metabolite trafficking, would prevent local trapping of labeled metabolites of [6-14C]glucose and cause underestimation of calculated CMRglc. Because the identities of glucose metabolites lost from activated structures and major routes of their removal are not known, arteriovenous differences across brains of conscious normoxic rats for derivatives of [6-14C]glucose were determined under steady-state conditions in blood during K+-induced spreading cortical depression. Lactate was identified as the maj
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Zamanian, Kazem, Konstantin Pustovoytov, and Yakov Kuzyakov. "Carbon Sources in Fruit Carbonate of Buglossoides arvensis and Consequences for 14C Dating." Radiocarbon 59, no. 1 (2017): 141–50. http://dx.doi.org/10.1017/rdc.2016.123.

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AbstractFruit carbonate of Buglossoides arvensis (syn. Lithospermum arvense) is a valuable dating and paleoenvironmental proxy for late Quaternary deposits and cultural layers because CaCO3 in fruit is assumed to be accumulated from photosynthetic carbon (C). However, considering the uptake of HCO3– by roots from soil solution, the estimated age could be too old depending on the source of HCO3– allocated in fruit carbonate. Until now, no studies have assessed the contributions of photosynthetic and soil C to the fruit carbonate. To evaluate this, the allocation of photo-assimilated carbon and
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Sanaullah, Muhammad, Abad Chabbi, Cornelia Rumpel, and Yakov Kuzyakov. "Carbon allocation in grassland communities under drought stress followed by 14C pulse labeling." Soil Biology and Biochemistry 55 (December 2012): 132–39. http://dx.doi.org/10.1016/j.soilbio.2012.06.004.

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Utsab, Thapa, and Adhikari Chettri Deepika. "Case research on the effects of drought on carbon assimilation within the plant-soil system." International Journal of Advance Research in Multidisciplinary 2, no. 4 (2024): 15–24. https://doi.org/10.5281/zenodo.13948832.

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Global warming is one of the biggest challenges, the world is facing at the moment and with the current rate of warming, serious consequences to climate have been predicted. Climate change may affect the ecosystem functioning through increased temperatures or changes in precipitation patterns. Water availability is an important driver for various ecosystem processes that may affect the supply of carbon in below-ground pools and alter important ecosystem processes involved in carbon cycling. The objective of this study was to determine the effect of drought stress in the allocation of carbon in
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Kuzyakov, Y. "How to link soil C pools with CO<sub>2</sub> fluxes?" Biogeosciences Discussions 8, no. 1 (2011): 1947–83. http://dx.doi.org/10.5194/bgd-8-1947-2011.

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Abstract. Despite the importance of carbon (C) pools and CO2 fluxes in terrestrial ecosystems and especially in soils, as well as many attempts to assign fluxes to specific pools, this challenge remains unsolved. Interestingly, scientists investigating pools are not closely linked with scientists studying fluxes. This mini-review therefore focused on experimental approaches enabling soil C pools to be linked with CO2 flux from the soil. The background, advantages and shortcomings of uncoupled approaches (measuring only pools or fluxes) and of coupled approaches (measuring both pools and fluxes
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Kuzyakov, Y. "How to link soil C pools with CO<sub>2</sub> fluxes?" Biogeosciences 8, no. 6 (2011): 1523–37. http://dx.doi.org/10.5194/bg-8-1523-2011.

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Abstract. Despite the importance of carbon (C) pools and CO2 fluxes in terrestrial ecosystems and especially in soils, as well as many attempts to assign fluxes to specific pools, this challenge remains unsolved. Interestingly, scientists investigating pools are not closely linked with scientists studying fluxes. This review therefore focused on experimental approaches enabling soil C pools to be linked with CO2 flux from the soil. The background, advantages and shortcomings of uncoupled approaches (measuring only pools or fluxes) and of coupled approaches (measuring both pools and fluxes) wer
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Olsson, I., M. Lantz, AM Persson, and K. Arnljots. "Biosynthesis and processing of lactoferrin in bone marrow cells, a comparison with processing of myeloperoxidase." Blood 71, no. 2 (1988): 441–47. http://dx.doi.org/10.1182/blood.v71.2.441.441.

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Abstract The processing and intracellular transport of lactoferrin of the neutrophil specific granules was investigated by biosynthetic labeling with (14C)leucine of bone marrow cells from healthy individuals and patients with chronic myeloid leukemia. Lactoferrin was precipitated with antilactoferrin serum and the immunoprecipitates were analyzed by sodium dodecyl sulfate (SDS), polyacrylamide gel electrophoresis (PAGE) followed by fluorography. In contrast to myeloperoxidase of azurophil granules, lactoferrin was not synthesized as a larger precursor, and it was not found to be phosphorylate
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Dissertations / Theses on the topic "14C pulse labeling"

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Scharroba, Anika. "Small but powerful." Doctoral thesis, Humboldt-Universität zu Berlin, Lebenswissenschaftliche Fakultät, 2017. http://dx.doi.org/10.18452/17798.

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In der vorliegenden Arbeit wurden die Einflüsse der landwirtschaftlichen Nutzung auf die Struktur und Biomasse von Nematoden entlang eines Tiefengradientens und innerhalb zweier Vegetationsperioden an einem Ackerstandort untersucht. Die Freilandanalyse der Nematodengemeinschaft wies auf ein mit Nährstoffen angereichertes und gestörtes Ökosystem, mit einer geringen Diversität, hin. Entlang des Tiefengradienten bildeten die Nematoden Metacommunities, welche Umweltgegebenheiten wie Nahrungsquellen und abiotischen Faktoren widerspiegeln. Signifikant höhere Biomassen wurden unter Weizen im Verglei
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Tahir, Majid Mahmood. "Destino do carbono de raízes e parte aéreas de culturas de inverno enriquecidas com 13c em solo sob plantio direto." Universidade Federal de Santa Maria, 2015. http://repositorio.ufsm.br/handle/1/3369.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>Little is known about the decomposition and stabilization of shoots and intact roots derived carbon (C) under no-tillage (no-till) field conditions. The present study was designed with following objectives: 1) evaluation of 13C pulse labeling method to label crop plants under field conditions for subsequent C dynamics studies, 2) estimation of the actual rates of mineralization of intact roots and shoot residues, decomposing simultaneously, and 3) finally to determine the fate of shoot vs root residues derived C in soil, under no
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Batalla, Juan Daniel Kennedy. "Tracing organic carbon cycling in agricultural soils of different catch crop management systems by 13C pulse labelling." Master's thesis, 2018. http://hdl.handle.net/10451/36327.

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Tese de mestrado, Ecologia e Gestão Ambiental, Universidade de Lisboa, Faculdade de Ciências, 2018<br>The loss of nutrients from agricultural soils leads to environmental problems and the infraction of environmental laws. Catch crops are a type of cover crop that are used in rotations as a way of avoiding soil nutrients from leaching. These crops may enhance other properties of the soil regarding quality and biological activity. The aim of this thesis is to explore these benefits by comparing fallow soil, to that of a single-species catch crop (Mustard) and the soils of two multi-species catch
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Books on the topic "14C pulse labeling"

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Risk-based food inspection manual for the Caribbean. Organización Panamericana de la Salud, 2019. http://dx.doi.org/10.37774/9789275121238.

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[Introduction] This manual contains guidance for risk-based inspections of food processing, preparation, retail and restaurants that countries can consult and adapt/adopt in developing a risk-based food business inspection program for their specific context. It is intended to help countries implement risk-based inspection systems that are consistent with international standards. This document builds on the FAO Risk Based Food Inspection manual (2008) and draws on the more recent guidance developed for governments by Codex Alimentarius, in particular, the Principles and Guidelines for National
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Book chapters on the topic "14C pulse labeling"

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Pianelli, Katia, Antoine Monier, Marie-Hélène Andrieu, Bertrand Beauvoit, and Martine Dieuaide-Noubhani. "14C Pulse Labeling to Estimate External Fluxes and Turnovers in Primary Metabolism." In Plant Metabolic Flux Analysis. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-688-7_3.

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Kramer, Gertjan, Piotr T. Kasper, Luitzen de Jong, and Chris G. de Koster. "Quantitation of Newly Synthesized Proteins by Pulse Labeling with Azidohomoalanine." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-148-2_12.

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Cotrufo, M. Francesca, and Yamina Pressler. "Heavy Isotope Enrichments." In A Primer on Stable Isotopes in Ecology. Oxford University PressOxford, 2023. http://dx.doi.org/10.1093/oso/9780198854494.003.0005.

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Abstract “Heavy Isotope Enrichments” reviews concepts for developing and interpreting heavy isotope labeling studies in ecology. The chapter compares continuous- and pulse-labeling approaches for the 13C labeling of plant material. Methods for the application of isotope-labeled substrates and their use in stable isotope probing of biological markers are presented. Calculations of the heavy isotope excess and the quantification of the recovery of heavy isotope additions in isotope tracing studies are provided. Principles and equations for the application of the isotope dilution method are illustrated, and its limitations are discussed. Examples of applications for carbon, nitrogen, and water stable isotopes are presented.
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