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1

Moran, Antoinette, Gianna Toffolo, Michele Schiavon, et al. "A novel triple-tracer approach to assess postprandial protein turnover." American Journal of Physiology-Endocrinology and Metabolism 315, no. 4 (2018): E469—E477. http://dx.doi.org/10.1152/ajpendo.00012.2018.

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Insulin and nutrients have profound effects on proteome homeostasis. Currently no reliable methods are available to measure postprandial protein turnover. A triple-tracer method was developed using phenylalanine stable isotope tracers to estimate appearance rates of ingested (Ra meal) and endogenous phenylalanine and the rate of phenylalanine disposal (Rd). This was compared with the “traditional” dual-tracer method, using one (1-CM)- and two (2-CM)-compartment models. For both methods, [13C6]phenylalanine was given orally, and [15N]phenylalanine was constantly infused; the triple-tracer metho
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2

Lewicka-Szczebak, Dominika, and Reinhard Well. "The <sup>15</sup>N gas-flux method to determine N<sub>2</sub> flux: a comparison of different tracer addition approaches." SOIL 6, no. 1 (2020): 145–52. http://dx.doi.org/10.5194/soil-6-145-2020.

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Abstract. The 15N gas-flux method allows for the quantification of N2 flux and tracing soil N transformations. An important requirement for this method is a homogeneous distribution of the 15N tracer added to soil. This is usually achieved through soil homogenization and admixture of the 15N tracer solution or multipoint injection of tracer solution to intact soil. Both methods may create artefacts. We aimed at comparing the N2 flux determined by the gas-flux method using both tracer distribution approaches. Soil incubation experiments with silt loam soil using (i) intact soil cores injected w
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3

Axler, R. P., and J. E. Reuter. "A Simple Method for Estimating the 15N Content of Dissolved Organic Matter (DO15N) in N-Cycling Studies." Canadian Journal of Fisheries and Aquatic Sciences 44, no. 1 (1987): 130–33. http://dx.doi.org/10.1139/f87-016.

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A method is described for isolating the total pool of dissolved organic nitrogen (DON) from oligotrophic lake water for subsequent isotopic analysis of the15N/14N ratio in tracer experiments. The procedure involves reducing an acidified filtered sample with elemental zinc to convert nitrate + nitrite to ammonium and then analyzing and sparging with N2 to remove ammonia. The residue (DON) is evaporated to dryness, rehydrated, spotted on a filter, and vacuum ampulated for micro-Dumas conversion to N2 and isotopic analysis by optical emission spectrometry. We have used the method to accurately es
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4

Sandrock, David R., Timothy L. Righetti, and Anita N. Azarenko. "Isotopic and Nonisotopic Estimation of Nitrogen Uptake Efficiency in Container-grown Woody Ornamentals." HortScience 40, no. 3 (2005): 665–69. http://dx.doi.org/10.21273/hortsci.40.3.665.

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Cornus sericea L., Weigela florida (Bunge) A. DC., and Euonymus alatus (Thunb.) Sieb were grown outside in 3.8-L plastic containers for 345 days (1 Apr. 2001 to 11 Mar. 2002). Nitrogen (N) was applied at rates (NAR) of 25, 50, 100, 200, and 300 mg·L–1 and delivered as aqueous double-labeled 15N depleted NH4NO3 (min 99.95% atom 14N). In all species, root, shoot, and total plant dry weight increased with increasing NARs while root to shoot ratios decreased. Similarly, root, shoot, and total plant N increased with NAR for each species, and at each NAR more N was stored in the roots than in the sh
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5

Dimitrova, Tsvetelina, Frank Repmann, and Dirk Freese. "Preparation of 15N-labeled Potassium Ferrocyanide for Tracer Studies." Environment and Pollution 6, no. 2 (2017): 41. http://dx.doi.org/10.5539/ep.v6n2p41.

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Isotopic labels are widely used to trace the fate and cycling of common environmental contaminants. Many of the labeled materials are not available commercially and, depending on the complexity of the substance, the label and the enrichment level, custom syntheses are costly. A simple, straightforward, and cost effective method for the preparation of a highly enriched, 15N-labeled potassium ferrocyanide (K4[Fe(C15N)6]*3H2O) has been developed to meet the requirements of related tracer experiments and minimize their costs. In this case, the 15N label was used to quantify iron cyanide detoxifica
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6

Tessier, Romain, Juliane Calvez, Nadezda Khodorova, et al. "Confrontation of the “Dual Tracer” Indirect Method With Direct Ileal Sampling for Indispensable Amino Acid Digestibility of Sunflower Isolate in Humans." Current Developments in Nutrition 5, Supplement_2 (2021): 884. http://dx.doi.org/10.1093/cdn/nzab048_019.

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Abstract Objectives The direct assessment of ileal samples of amino acid (AA) digestibility is invasive in humans. A less invasive but indirect method, namely « dual tracer » was recently developed. It relies on the plasma isotopic enrichment ratio of two labeled protein, a reference protein or alternately AAs labeled with 13C and the test protein labeled with 15N. This recent method has not yet been challenged against direct measurement of ileal digestibility. Methods Seven healthy volunteers were intubated with naso-ileal tube. Every 30min for 4h, they ingested sunflower biscuits containing
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7

KIMURA, Motoi, Muneoki YOH, Hiroko AKIYAMA, and Masaaki HOSOMI. "Performance of Nitrogen Removal in Reed Filter Bed System Using 15N Tracer Method." Journal of Japan Society on Water Environment 23, no. 11 (2000): 703–9. http://dx.doi.org/10.2965/jswe.23.703.

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8

Bronk, DA, and PM Glibert. "A 15N tracer method for the measurement of dissolved organic nitrogen release by phytoplankton." Marine Ecology Progress Series 77 (1991): 171–82. http://dx.doi.org/10.3354/meps077171.

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9

Tavares, Claudinéia R. O., José A. Bendassolli, Fernando Coelho, Carlos R. Sant'ana Filho, and Clelber V. Prestes. "15N-labed glycine synthesis." Anais da Academia Brasileira de Ciências 78, no. 3 (2006): 441–49. http://dx.doi.org/10.1590/s0001-37652006000300005.

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This work describes a method for 15N-isotope-labeled glycine synthesis, as well as details about a recovery line for nitrogen residues. To that effect, amination of alpha-haloacids was performed, using carboxylic chloroacetic acid and labeled aqueous ammonia (15NH3). Special care was taken to avoid possible 15NH3 losses, since its production cost is high. In that respect, although the purchase cost of the 13N-labeled compound (radioactive) is lower, the stable tracer produced constitutes an important tool for N cycling studies in living organisms, also minimizing labor and environmental hazard
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10

Sgouridis, Fotis, Andrew Stott, and Sami Ullah. "Application of the <sup>15</sup>N gas-flux method for measuring in situ N<sub>2</sub> and N<sub>2</sub>O fluxes due to denitrification in natural and semi-natural terrestrial ecosystems and comparison with the acetylene inhibition technique." Biogeosciences 13, no. 6 (2016): 1821–35. http://dx.doi.org/10.5194/bg-13-1821-2016.

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Abstract. Soil denitrification is considered the most un-constrained process in the global N cycle due to uncertain in situ N2 flux measurements, particularly in natural and semi-natural terrestrial ecosystems. 15N tracer approaches can provide in situ measurements of both N2 and N2O simultaneously, but their use has been limited to fertilized agro-ecosystems due to the need for large 15N additions in order to detect 15N2 production against the high atmospheric N2. For 15N–N2 analyses, we have used an “in-house” laboratory designed and manufactured N2 preparation instrument which can be interf
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11

Moreira-Coello, Víctor, Beatriz Mouriño-Carballido, Emilio Marañón, Ana Fernández-Carrera, María PÉrez-Lorenzo, and Antonio Bode. "Quantifying the overestimation of planktonic N2 fixation due to contamination of 15N2 gas stocks." Journal of Plankton Research 41, no. 4 (2019): 567–70. http://dx.doi.org/10.1093/plankt/fbz034.

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AbstractThe 15N2-tracer assay [Montoya et al. (1996) A simple, high-precision, high-sensitivity tracer assay for N2 fixation. Appl. Environ. Microbiol., 62, 986–993.] is the most used method for measuring biological N2 fixation in terrestrial and aquatic environments. The reliability of this technique depends on the purity of the commercial 15N2 gas stocks used. However, Dabundo et al. [(2014) PLoS One, 9, e110335.] reported the contamination of some of these stocks with labile 15N-labeled compounds (ammonium, nitrate and/or nitrite). The contamination of commercial 15N2 gas stocks with 15N-la
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12

Guillin, Florence, Claire Gaudichon, Laetitia Guerin-Deremaux, et al. "Comparison of Dual Isotope and Ileal Sampling Methods for the Determination of the Amino Acid Digestibility of Pea Protein and Casein in Adult Humans." Current Developments in Nutrition 5, Supplement_2 (2021): 873. http://dx.doi.org/10.1093/cdn/nzab048_008.

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Abstract Objectives The measurement of amino acid (AA) digestibility of protein through direct ileal sampling is highly invasive and inappropriate for vulnerable populations, such as children or elderly. The new dual tracer method relies on comparing meal and plasma isotopic ratios of 1/a test protein 2/a reference protein (or AA mix) of known digestibility, each one being labelled with a different isotope. The aim of this study was to compare this new indirect dual tracer method to standard ileal method, for the determination of AA digestibility of pea protein and milk casein. Methods Fifteen
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13

KOWALENKO, C. G. "THE FATE OF APPLIED NITROGEN IN A FRASER VALLEY SOIL USING 15N IN FIELD MICROPLOTS." Canadian Journal of Soil Science 69, no. 4 (1989): 825–33. http://dx.doi.org/10.4141/cjss89-083.

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A tracer (15N) study using fallowed field microplots was conducted at Agassiz Research Station to examine the fate of applied N over an entire year. The tracer confirmed nontracer (difference between fertilized and control treatments) observations that applied N does not leach beyond the rooting zone (45 cm) during the growing season, despite the considerably more than average precipitation that occurred in July, but that all residual [Formula: see text] is leached over the winter. The tracer did, however, show that net immobilization of applied N occurred late in the fall resulting in 17% of
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14

Hosomi, M., S. Nakai, M. Kimura, M. Yoh, and H. Akiyama. "Evaluation of Nitrogen Removal Potential in a Reed Filter Bed System by the 15N Tracer Method." Acta Biotechnologica 22, no. 1-2 (2002): 55–66. http://dx.doi.org/10.1002/1521-3846(200205)22:1/2<55::aid-abio55>3.0.co;2-q.

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15

Smith, Thomas Brendan, Kamlesh Patel, Haydn Munford, et al. "High-Speed Tracer Analysis of Metabolism (HS-TrAM)." Wellcome Open Research 3 (August 22, 2018): 5. http://dx.doi.org/10.12688/wellcomeopenres.13387.2.

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Tracing the fate of stable isotopically-enriched nutrients is a sophisticated method of describing and quantifying the activity of metabolic pathways. Nuclear Magnetic Resonance (NMR) spectroscopy offers high resolution data in terms of resolving metabolic pathway utilisation. Despite this, NMR spectroscopy is under-utilised due to length of time required to collect the data, quantification requiring multiple samples and complicated analysis. Here we present two techniques, quantitative spectral filters and enhancement of the splitting of 13C signals due to homonuclear 13C,13C or heteronuclear
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16

Suzuki, Marcelino T., Evelyn B. Sherr, and Barry F. Sherr. "Estimation of ammonium regeneration efficiencies associated with bacterivory in pelagic food webs via a 15N tracer method." Journal of Plankton Research 18, no. 3 (1996): 411–28. http://dx.doi.org/10.1093/plankt/18.3.411.

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17

Wozniak, Jeffrey R., Daniel L. Childers, William T. Anderson, David T. Rudnick, and Christopher J. Madden. "An in situ mesocosm method for quantifying nitrogen cycling rates in oligotrophic wetlands using 15N tracer techniques." Wetlands 28, no. 2 (2008): 502–12. http://dx.doi.org/10.1672/07-29.1.

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18

Sgouridis, F., S. Ullah, and A. Stott. "Application of the <sup>15</sup>N-Gas Flux method for measuring in situ N<sub>2</sub> and N<sub>2</sub>O fluxes due to denitrification in natural and semi-natural terrestrial ecosystems and comparison with the acetylene inhibition technique." Biogeosciences Discussions 12, no. 15 (2015): 12653–89. http://dx.doi.org/10.5194/bgd-12-12653-2015.

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Abstract. Soil denitrification is considered the most un-constrained process in the global N cycle due to uncertain in situ N2 flux measurements, particularly in natural and semi-natural terrestrial ecosystems. 15N tracer approaches can provide in situ measurements of both N2 and N2O simultaneously, but their use has been limited to fertilised agro-ecosystems due to the need for large 15N additions in order to detect 15N2 production against the high atmospheric N2. For 15N-N2 analyses, we have used an "in house" laboratory designed and manufactured N2 preparation instrument which can be interf
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19

Eickenscheidt, N., R. Brumme, and E. Veldkamp. "Direct contribution of nitrogen deposition to nitrous oxide emissions in a temperate beech and spruce forest – a <sup>15</sup>N tracer study." Biogeosciences 8, no. 3 (2011): 621–35. http://dx.doi.org/10.5194/bg-8-621-2011.

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Abstract. The impact of atmospheric nitrogen (N) deposition on nitrous oxide (N2O) emissions in forest ecosystems is still unclear. Our study assessed the direct contribution of N deposition to N2O emissions in temperate forests exposed to chronic high N depositions using a 15N labelling technique. In a Norway spruce stand (Picea abies) and in a beech stand (Fagus sylvatica) at the Solling, Germany, we used a low concentrated 15N-labelled ammonium-nitrate solution to simulate N deposition. Nitrous oxide fluxes and 15N isotope abundances in N2O were measured using the closed chamber method comb
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20

Eickenscheidt, N., R. Brumme, and E. Veldkamp. "Direct contribution of nitrogen deposition to nitrous oxide emissions in a temperate beech and spruce forest – a <sup>15</sup>N tracer study." Biogeosciences Discussions 7, no. 6 (2010): 8345–79. http://dx.doi.org/10.5194/bgd-7-8345-2010.

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Abstract. The impact of atmospheric nitrogen (N) deposition on nitrous oxide (N2O) emissions in forest ecosystems is still unclear. The objective of our study was to investigate the direct contribution of N deposition to N2O emissions in temperate forests exposed to chronic high N deposition using a 15N labelling technique. In a Norway spruce stand (Picea abies) and in a beech stand (Fagus sylvatica) in the Solling, Germany, we added a low concentrated 15N-labelled ammoniumnitrate solution to simulate N deposition. Nitrous oxide fluxes and 15N isotope abundances in N2O were measured using the
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21

Xu, Min Nina, Yanhua Wu, Li Wei Zheng, et al. "Quantification of multiple simultaneously occurring nitrogen flows in the euphotic ocean." Biogeosciences 14, no. 4 (2017): 1021–38. http://dx.doi.org/10.5194/bg-14-1021-2017.

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Abstract. The general features of the N cycle in the sunlit region of the ocean are well known, but methodological difficulties have previously confounded simultaneous quantification of transformation rates among the many different forms of N, e.g., ammonium (NH4+), nitrite (NO2−), nitrate (NO3−), and particulate/dissolved organic nitrogen (PN/DON). However, recent advances in analytical methodology have made it possible to employ a convenient isotope labeling technique to quantify in situ fluxes among oft-measured nitrogen species within the euphotic zone. Addition of a single 15N-labeled NH4
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22

Nagao, F., and T. Miyazaki. "A modified 15N tracer method and new calculation for estimating release of dissolved organic nitrogen by freshwater planktonic algae." Aquatic Microbial Ecology 16 (1999): 309–14. http://dx.doi.org/10.3354/ame016309.

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23

EBISSA, Tchister, Bo Yang, Yuanqing Guan, et al. "A Comparison of Nitrogen Transfer and Transformation in Traditional Farming and the Rice–Duck Farming System by 15N Tracer Method." Agronomy 8, no. 12 (2018): 289. http://dx.doi.org/10.3390/agronomy8120289.

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A field experiment was conducted in Ninghe, Tianjin, China, using the 15N isotope method to determine the fate of N sources, application effect of organic fertilizer on the growth of rice plant organs, N uptake by rice, and N use efficiency. The experiment included eight treatments: CK-N (control + no-duck), CK-D (control + ducks), CF-N (chemical fertilizer + no-ducks), CF-D (chemical fertilizer + ducks), CM-N (chemical fertilizer + organic fertilizer + no-ducks), CM-D (chemical fertilizer + organic fertilizer + ducks), CD-N (chemical fertilizer 30% off + organic fertilizer + no-ducks), and CD
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24

Smith, Thomas Brendan, Kamlesh Patel, Haydn Munford, et al. "High-Speed Tracer Analysis of Metabolism (HS-TrAM)." Wellcome Open Research 3 (January 12, 2018): 5. http://dx.doi.org/10.12688/wellcomeopenres.13387.1.

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Tracing the fate of stable isotopically-enriched nutrients is a sophisticated method of describing and quantifying the activity of metabolic pathways. Nuclear Magnetic Resonance (NMR) offers high resolution data, yet is under-utilised due to length of time required to collect the data, quantification requiring multiple samples and complicated analysis. Here we present two techniques, quantitative spectral filters and enhancement of the splitting due to J-coupling in 1H,13C-HSQC NMR spectra, which allow the rapid collection of NMR data in a quantitative manner on a single sample. The reduced du
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25

Khalifa, K., and F. Kurdali. "N2fixation in faba bean grown under saline and non-saline conditions using a 15N-tracer technique." Acta Agronomica Hungarica 53, no. 4 (2005): 393–403. http://dx.doi.org/10.1556/aagr.53.2005.4.5.

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A pot experiment was conducted to study the performance of faba bean and barley growing under saline conditions, in terms of dry matter yield, total N, and percentages and amount of N derived from the soil, fertilizer and atmosphere using a 15N isotope dilution method. Three saline treatments were employed: 1) plants were grown on saline soil and irrigated with saline water (SsWs); 2) plants were grown on saline soil and irrigated with non-saline water (SsWn); and 3) plants were grown on non-saline soil and irrigated with saline water (SnWs). Furthermore, a control treatment was employed by gr
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26

Bronk, D. A., and P. M. Glibert. "Application of a 15N tracer method to the study of dissolved organic nitrogen uptake during spring and summer in Chesapeake Bay." Marine Biology 115, no. 3 (1993): 501–8. http://dx.doi.org/10.1007/bf00349849.

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27

Sun, Yongjian, Yuanyuan Sun, Fengjun Yan, et al. "Coordinating Postanthesis Carbon and Nitrogen Metabolism of Hybrid Rice through Different Irrigation and Nitrogen Regimes." Agronomy 10, no. 8 (2020): 1187. http://dx.doi.org/10.3390/agronomy10081187.

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We sought to explore the role of postanthesis carbon and nitrogen (C-N) metabolism of hybrid rice in increasing yield and nitrogen utilization efficiency (NUE). We used the 13C and 15N dual-isotope tracer method and physiological/biochemical analysis and established different irrigation and nitrogen fertilization (W-N) regimes to investigate the relationship of C-N metabolism characteristics, yield, and NUE. The results showed that W-N regimes had significant effects on postanthesis absorption and translocation of N and photosynthate, yield and NUE. Aerobic irrigation combined with the N ferti
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28

Kumar, S., R. Ramesh, S. Sardesai, and M. S. Sheshshayee. "Effect of incubation time and substrate concentration on N-uptake rates by phytoplankton in the Bay of Bengal." Biogeosciences Discussions 2, no. 5 (2005): 1331–52. http://dx.doi.org/10.5194/bgd-2-1331-2005.

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Abstract. We report here the results of three experiments, which are slight variations of the 15N method (JGOFS protocol) for determination of new production. The first two test the effect of (i) duration of incubation time and (ii) concentration of tracer added on the uptake rates of various N-species (nitrate, ammonium and urea) by marine phytoplankton; while the third compares in situ and deck incubations from dawn to dusk. Results indicate that nitrate uptake can be underestimated by experiments where incubation times shorter than 4h or when more than 10% of the ambient concentration of ni
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Marsh, Kerri L., Richard L. Mulvaney та Gerald K. Sims. "A Technique to Recover Tracer as Carboxyl–Carbon and α-Nitrogen from Amino Acids in Soil Hydrolysates". Journal of AOAC INTERNATIONAL 86, № 6 (2003): 1106–11. http://dx.doi.org/10.1093/jaoac/86.6.1106.

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Abstract Isotope analysis of biochemical compounds provides an unequivocal means for detecting assimilation of tracer C and N into microbial biomass. A diffusion method recently developed to determine amino acid-N by ninhydrin oxidation of soil hydrolysates was modified to permit simultaneous collection of the CO2 liberated during this oxidation. In the technique described herein, this is accomplished after removal of (NH4+ + amino sugar)–N, by performing ninhydrin oxidation at 90°C for 7 h in a 1.9 L Mason jar sealed with a lid equipped to support a petri dish containing 5 mL 0.2N NaOH. Recov
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30

Hristov, A. N., T. A. McAllister, D. R. Ouellet, and G. A. Broderick. "Comparison of purines and nitrogen-15 as microbial flow markers in beef heifers fed barley- or corn-based diets." Canadian Journal of Animal Science 85, no. 2 (2005): 211–22. http://dx.doi.org/10.4141/a04-054.

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The objective of this study was to estimate the contribution of microbial purine bases to duodenal purines and to purine derivatives [allantoin and uric acid (PD)] excreted in the urine. Additionally, microbial protein (MCP) flow estimated using duodenal flow of purine bases was compared to estimates using 15N as a microbial marker. Four beef heifers were fed two diets, barley silage/barley grain/soybean meal (diet B) or corn silage/corn grain/corn gluten meal (diet C), in a cross-over design study. (15NH4)2SO4 was infused in the rumen for 8 d to label ruminal microorganisms and their purine b
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Lima, Vania Luzia Fournou de, Carlos Ducatti, Paulo Roberto de Lima Meirelles, et al. "Isotopic of nitrogen and chemical analysis of deferred grazing marandu palisadegrass fertilized with urea and poultry litter." Semina: Ciências Agrárias 37, no. 4Supl1 (2016): 2609. http://dx.doi.org/10.5433/1679-0359.2016v37n4supl1p2609.

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This study aimed to evaluate and quantify the methodology of stable isotopes, 15N values for the organic fertilizer (chicken manure) and inorganic (urea). In addition to evaluating the incorporation rate, chemical half-life and analysis thereof, for possible traceability through the pasture fertilized with different sources of N. The technique of employment with use of tracer stable isotope 15N possible to estimate the changes in the N soil, even in the face of the various changes that take place concurrently. The methodology determines the path of both N isotopes (15N and 14N), providing info
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Tamele, Rosalina Armando, Hideto Ueno, Yo Toma, and Nobuki Morita. "Nitrogen Recoveries and Nitrogen Use Efficiencies of Organic Fertilizers with Different C/N Ratios in Maize Cultivation with Low-Fertile Soil by 15N Method." Agriculture 10, no. 7 (2020): 272. http://dx.doi.org/10.3390/agriculture10070272.

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The rising cost of inorganic fertilizers, coupled with their adverse effects on soil conditions, has resulted in increasing interest in organic amendments. The objective of the present study was to evaluate the effect of organic amendments (OAs) with different C/N ratios on nitrogen use efficiency (NUE) and recovery rate, as well as on the growth and yield of Zea mays and soil properties. A precise pot experiment was conducted on a low-fertile, sandy-loam soil, and the dynamics of nitrogen (N) were also analyzed by the A-value method, using 15N tracer. The plant height of the treatment groups
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33

Lyu, Xiaochen, Ming Li, Xin Li, et al. "Assessing the Systematic Effects of the Concentration of Nitrogen Supplied to Dual-Root Systems of Soybean Plants on Nodulation and Nitrogen Fixation." Agronomy 10, no. 6 (2020): 763. http://dx.doi.org/10.3390/agronomy10060763.

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The specific mechanisms by which nitrogen affects the nodulation and nitrogen fixation of soybean plants are unclear. Investigating the relationship between nitrogen, nodulation and nitrogen fixation can provide new insights for the rational and proper use of nitrogen fertilizer in soybean plants. In this study, we grafted soybean roots to construct a dual-root system with a single nodulated side. Experiment I was performed at the third trifoliate leaf to initial seed filling (V3-R3) growth stages (for 30 days) for long-term nitrogen supply, and Experiment II was performed at the third trifoli
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Tan, Zhongxin, Zhixiong Ye, Limei Zhang, and Qiaoyun Huang. "Application of the 15N tracer method to study the effect of pyrolysis temperature and atmosphere on the distribution of biochar nitrogen in the biomass–biochar-plant system." Science of The Total Environment 622-623 (May 2018): 79–87. http://dx.doi.org/10.1016/j.scitotenv.2017.11.341.

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35

Chien, P. F., K. Smith, P. W. Watt, C. M. Scrimgeour, D. J. Taylor, and M. J. Rennie. "Protein turnover in the human fetus studied at term using stable isotope tracer amino acids." American Journal of Physiology-Endocrinology and Metabolism 265, no. 1 (1993): E31—E35. http://dx.doi.org/10.1152/ajpendo.1993.265.1.e31.

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Before elective cesarean delivery (4 h), we infused L-[1-13C]leucine and L-[15N]phenylalanine into the maternal circulation and measured enrichment and concentration of amino acids and carbon dioxide in cord blood of six normal human fetuses at delivery. There were net fetal uptakes of leucine (2.22 +/- 0.29 mumol.kg-1.min-1) and phenylalanine (0.80 +/- 0.11 mumol.kg-1.min-1) with net outputs of CO2 (6.11 +/- 1.12 ml.kg-1.min-1) and the transamination product of leucine, alpha-ketoisocaproate (1.04 +/- 0.32 mumol.kg-1.min-1). Fetal amino acid oxidation accounted for a substantial proportion of
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36

Peng, Xuefeng, and David L. Valentine. "Diversity and N2O Production Potential of Fungi in an Oceanic Oxygen Minimum Zone." Journal of Fungi 7, no. 3 (2021): 218. http://dx.doi.org/10.3390/jof7030218.

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Fungi in terrestrial environments are known to play a key role in carbon and nitrogen biogeochemistry and exhibit high diversity. In contrast, the diversity and function of fungi in the ocean has remained underexplored and largely neglected. In the eastern tropical North Pacific oxygen minimum zone, we examined the fungal diversity by sequencing the internal transcribed spacer region 2 (ITS2) and mining a metagenome dataset collected from the same region. Additionally, we coupled 15N-tracer experiments with a selective inhibition method to determine the potential contribution of marine fungi t
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Guo, Lijun, Jiusheng Li, Yanfeng Li, and Di Xu. "Nitrogen Availability of Sewage Effluent to Maize Compared to Synthetic Fertilizers under Surface Drip Irrigation." Transactions of the ASABE 61, no. 4 (2018): 1365–77. http://dx.doi.org/10.13031/trans.12779.

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Abstract. Field experiments on maize ( L.) under surface drip irrigation were carried out during two consecutive growing seasons to examine the influence of water quality (groundwater and secondary sewage effluent) and nitrogen (N) application rate on growth and yield, N agronomic efficiency, and N availability of effluent. Pot experiments were conducted using the 15N isotope tracer method and the fertilizer equivalence (FE) approach to provide direct evidence of the effluent N availability derived from the field experiments. Four N application rates ranging from 0 to 180 kg ha-1 for field exp
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Yamagishi, Takao, Mio Takeuchi, Yuichiro Wakiya, and Miyoko Waki. "Distribution and characterization of anammox in a swine wastewater activated sludge facility." Water Science and Technology 67, no. 10 (2013): 2330–36. http://dx.doi.org/10.2166/wst.2013.102.

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Anaerobic ammonium oxidation (anammox) is a novel biological nitrogen removal process that oxidizes NH4+ to N2 with NO2− as an electron acceptor. The purpose of this study was to examine the potential activity and characteristics of anammox in a conventional swine wastewater treatment facility, which uses an activated sludge system consisting of three cascade aeration tanks equipped with ceramic support material. Anammox activity was estimated by a 15N tracer assay method and was detected in all the sludge and biofilm samples in each aeration tank. Biofilm taken from the third aeration tank, i
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Rasmussen, Camilla Ruø, Kristian Thorup-Kristensen, and Dorte Bodin Dresbøll. "The effect of drought and intercropping on chicory nutrient uptake from below 2 m studied in a multiple tracer setup." Plant and Soil 446, no. 1-2 (2019): 543–61. http://dx.doi.org/10.1007/s11104-019-04348-8.

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Abstract Aims We tested if chicory acquires nutrients from soil layers down to 3.5 m depth and whether the deep nutrient uptake increases as a result of drought or intercropping with ryegrass and black medic. We also tested whether application of the trace elements Cs, Li, Rb, Sr and Se, as tracers, result in similar uptake rates. Methods The methodological tests were carried out in a pilot experiment where the tracers were applied to 1 m depth in lucerne and red beet grown in tube rhizotrons. The dynamics of deep nutrient uptake in chicory was studied in large 4 m deep rhizoboxes. A drought w
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Poupin, Nathalie, Jean-François Huneau, François Mariotti, Daniel Tomé, Cécile Bos, and Hélène Fouillet. "Isotopic and modeling investigation of long-term protein turnover in rat tissues." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 304, no. 3 (2013): R218—R231. http://dx.doi.org/10.1152/ajpregu.00310.2012.

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Fractional synthesis rates (FSR) of tissue proteins (P) are usually measured using labeled amino acid (AA) tracer methods over short periods of time under acute, particular conditions. By combining the long-term and non-steady-state 15N labeling of AA and P tissue fractions with compartmental modeling, we have developed a new isotopic approach to investigate the degree of compartmentation of P turnover in tissues and to estimate long-term FSR values under sustained and averaged nutritional and physiological conditions. We measured the rise-to-plateau kinetics of nitrogen isotopic enrichments (
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Carter, C., S. Owen, Z. He, et al. "DETERMINATION OF PROTEIN SYNTHESIS IN RAINBOW TROUT, ONCORHYNCHUS MYKISS, USING A STABLE ISOTOPE." Journal of Experimental Biology 189, no. 1 (1994): 279–84. http://dx.doi.org/10.1242/jeb.189.1.279.

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It has been suggested (Houlihan, 1991) that the consumption of 1 g of protein in a variety of species of fish stimulates the synthesis of, approximately, an equal amount of protein. Although synthesis of protein may account for as much as 40 % of the whole-animal oxygen consumption (Lyndon et al. 1992), only about 30 % of the synthesized proteins are retained as growth (Houlihan et al. 1988; Carter et al. 1993a,b). Thus, one focus of attention is the potential advantage gained by fish in allocating a considerable proportion of assimilated energy to protein turnover in contrast to relatively lo
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Rafii, Mahroukh, Jane M. McKenzie, Susan A. Roberts, George Steiner, Ronald O. Ball, and Paul B. Pencharz. "In vivo regulation of phenylalanine hydroxylation to tyrosine, studied using enrichment in apoB-100." American Journal of Physiology-Endocrinology and Metabolism 294, no. 2 (2008): E475—E479. http://dx.doi.org/10.1152/ajpendo.00604.2007.

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Phenylalanine hydroxylation is necessary for the conversion of phenylalanine to tyrosine and disposal of excess phenylalanine. Studies of in vivo regulation of phenylalanine hydroxylation suffer from the lack of a method to determine intrahepatocyte enrichment of phenylalanine and tyrosine. apoB-100, a hepatic export protein, is synthesized from intrahepatocyte amino acids. We designed an in vivo multi-isotope study, [15N]phenylalanine and [2H2]tyrosine to determine rates of phenylalanine hydroxylation from plasma enrichments in free amino acids and apoB-100. For independent verification of ap
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Redaelli, E., L. Bizzocchi, P. Caselli, et al. "14N/15N ratio measurements in prestellar cores with N2H+: new evidence of 15N-antifractionation." Astronomy & Astrophysics 617 (September 2018): A7. http://dx.doi.org/10.1051/0004-6361/201833065.

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Context. The 15N fractionation has been observed to show large variations among astrophysical sources, depending both on the type of target and on the molecular tracer used. These variations cannot be reproduced by the current chemical models. Aims. Until now, the 14N/15N ratio in N2H+ has been accurately measured in only one prestellar source, L1544, where strong levels of fractionation, with depletion in 15N, are found (14N/15N ≈ 1000). In this paper, we extend the sample to three more bona fide prestellar cores, in order to understand if the antifractionation in N2H+ is a common feature of
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Kulkarni, Madhura V., Amy J. Burgin, Peter M. Groffman, and Joseph B. Yavitt. "Direct flux and 15N tracer methods for measuring denitrification in forest soils." Biogeochemistry 117, no. 2-3 (2013): 359–73. http://dx.doi.org/10.1007/s10533-013-9876-7.

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Yi, Chang, Xinchong Shi, Donglan Yu, et al. "The combination of 13N-ammonia and 18F-FDG PET/CT in the identification of metabolic phenotype of primary human brain tumors." Nuklearmedizin 58, no. 03 (2019): 272–78. http://dx.doi.org/10.1055/a-0835-5746.

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Abstract Aim This pilot study made a preliminary attempt to distinguish different metabolic phenotypes of primary human brain tumors with dual tracer 13N-ammonia and 18F-FDG. Methods 74 patients were included in this study including 12 benign meningiomas (B-MEN), 4 malignant meningiomas (M-MEN), 15 low-grade gliomas (LGG), 32 high-grade gliomas (HGG) and 11 primary central nervous system lymphomas (PCNSL). All patients underwent 13N-ammonia and 18F-FDG PET imaging. Semi-quantification analysis by tumor-to-gray matter (T/G) ratios was used for the evaluation of tracer uptakes. After the calcula
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Zeng, Xian Yang, Zuo He Chi, and Ming Guang Zheng. "Calibration for Gas Flowmeter with Tracer Gas Dilution Method." Advanced Materials Research 204-210 (February 2011): 2154–57. http://dx.doi.org/10.4028/www.scientific.net/amr.204-210.2154.

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The principle of calibration for gas flowmeter with tracer gas dilution method is introduced, and experiments on calibration for gas flowmeter in a 0.300m internal diameter and 90 degree bent pipe are presented, which CO and air were selected as tracer gas and gas stream. The sampling points were downstream of the bend pipe around 9D (D is the internal diameter of pipe),12D and 15D, respectively. The results show that the radial and axial mixing between tracer gas and air have been uniformed downstream of the bend pipe at the sampling point of 12D and 15D when the flow rate above 3.2m/s, and t
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Li, Fei, Junzhi Wang, Min Fang, et al. "Isotopologues of dense gas tracers in nearby infrared bright galaxies." Monthly Notices of the Royal Astronomical Society 494, no. 1 (2020): 1095–113. http://dx.doi.org/10.1093/mnras/staa676.

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ABSTRACT We present 1 and 3 mm observations of the 13C- and 15N-bearing isotopologues of dense gas tracers towards eight nearby infrared-bright galaxies. With the Institut de Radioastronomie Millimétrique 30-m telescope, we observed the J = 1–0 transitions of H13CN, HC15N, H13CO+, HN13C, and H15NC towards M 82, NGC 3079, IC 694, Mrk 231, and NGC 6240. The J = 3–2 transition of H13CN was obtained in M 82, NGC 3079, NGC 3504, NGC 4418, NGC 6240, and NGC 6946, using the 10-m Submillimeter Telescope (SMT). We report the first detections of HN13C J = 1–0 and H13CN J = 3–2 in M 82, and H13CN J = 3–2
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Vicini, Paolo, Jeffrey J. Zachwieja, Kevin E. Yarasheski, Dennis M. Bier, Andrea Caumo, and Claudio Cobelli. "Glucose production during an IVGTT by deconvolution: validation with the tracer-to-tracee clamp technique." American Journal of Physiology-Endocrinology and Metabolism 276, no. 2 (1999): E285—E294. http://dx.doi.org/10.1152/ajpendo.1999.276.2.e285.

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Recently, a new method, based on a two-compartment minimal model and deconvolution [A. Caumo and C. Cobelli. Am. J. Physiol 264 ( Endocrinol. Metab.. 37): E829–E841, 1993; P. Vicini, G. Sparacino, A. Caumo, and C. Cobelli. Comput. Meth. Prog. Biomed. 52: 147–156, 1997], has been proposed to estimate endogenous glucose production (EGP) from labeled intravenous glucose tolerance test (IVGTT) data. Our aim here is to compare this EGP profile with that independently obtained with the reference method, based on the tracer-to-tracee ratio (TTR) clamp. An insulin-modified (0.03 U/kg body wt infused o
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Pabst, C. H. M., A. Hacar, J. R. Goicoechea та ін. "[C II] 158 μm line emission from Orion A I. A template for extragalactic studies?" Astronomy & Astrophysics 651 (липень 2021): A111. http://dx.doi.org/10.1051/0004-6361/202140804.

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Context. The [C II] 158 μm fine-structure line is one of the dominant coolants of the neutral interstellar medium. It is hence one of the brightest far-infrared (FIR) emission lines and can be observed not only in star-forming regions throughout the Galaxy, but also in the diffuse interstellar medium and in distant galaxies. [C II] line emission has been suggested to be a powerful tracer of star formation. Aims. We aim to understand the origin of [C II] emission and its relation to other tracers of interstellar gas and dust. This includes a study of the heating efficiency of interstellar gas a
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Chai, Yu, and Dong Mei Shang. "The Improvement of Maximum Power Point Tracker Algorithm in Photovoltaic Power System." Applied Mechanics and Materials 157-158 (February 2012): 377–80. http://dx.doi.org/10.4028/www.scientific.net/amm.157-158.377.

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Maximum power tracking is one of most important methods to improve efficiency of Photovoltaic power system. By simulating and analyzing the photovoltaic cell model,using Boost circuit to achieve maximum power tracking. Based on the analysis of the constant-voltage method, perturbation and observation method, then combining these two methods,I Put forward the constant-voltage method combined with perturbation and observation method, and has carried on the simulation.
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