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1

Cameron, Todd A., and Nicholas R. De Lay. "The Phosphorolytic Exoribonucleases Polynucleotide Phosphorylase and RNase PH Stabilize sRNAs and Facilitate Regulation of Their mRNA Targets." Journal of Bacteriology 198, no. 24 (2016): 3309–17. http://dx.doi.org/10.1128/jb.00624-16.

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ABSTRACT Gene regulation by base pairing between small noncoding RNAs (sRNAs) and their mRNA targets is an important mechanism that allows bacteria to maintain homeostasis and respond to dynamic environments. In Gram-negative bacteria, sRNA pairing and regulation are mediated by several RNA-binding proteins, including the sRNA chaperone Hfq and polynucleotide phosphorylase (PNPase). PNPase and its homolog RNase PH together represent the two 3′ to 5′ phosphorolytic exoribonucleases found in Escherichia coli ; however, the role of RNase PH in sRNA regulation has not yet been explored and reporte
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Subhadra, Bindu, Dianjun Cao, Roderick Jensen, Clayton Caswell, and Thomas J. Inzana. "Identification and initial characterization of Hfq-associated sRNAs in Histophilus somni strain 2336." PLOS ONE 18, no. 5 (2023): e0286158. http://dx.doi.org/10.1371/journal.pone.0286158.

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Small RNAs (sRNA), in association with the global chaperone regulator Hfq, positively or negatively regulate gene expression in bacteria. For this study, Histophilus somni sRNAs that bind to Hfq were identified and then partially characterized. The Hfq-associated sRNAs in H. somni were isolated and identified by co-immunoprecipitation using anti-Hfq antibody, followed by sRNA sequencing. Sequence analysis of the sRNA samples identified 100 putative sRNAs, out of which 16 were present in pathogenic strain 2336, but not in non-pathogenic strain 129Pt. Bioinformatic analyses suggested that the sR
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3

Barreto, Brittany, Elizabeth Rogers, Jun Xia, et al. "The Small RNA GcvB Promotes Mutagenic Break Repair by Opposing the Membrane Stress Response." Journal of Bacteriology 198, no. 24 (2016): 3296–308. http://dx.doi.org/10.1128/jb.00555-16.

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ABSTRACTMicrobes and human cells possess mechanisms of mutagenesis activated by stress responses. Stress-inducible mutagenesis mechanisms may provide important models for mutagenesis that drives host-pathogen interactions, antibiotic resistance, and possibly much of evolution generally. InEscherichia coli, repair of DNA double-strand breaks is switched to a mutagenic mode, using error-prone DNA polymerases, via the SOS DNA damage and general (σS) stress responses. We investigated small RNA (sRNA) clients of Hfq, an RNA chaperone that promotes mutagenic break repair (MBR), and found that GcvB p
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Raman, Vidhyavathi, Stacey A. Simon, Feray Demirci, Mayumi Nakano, Blake C. Meyers, and Nicole M. Donofrio. "Small RNA Functions Are Required for Growth and Development of Magnaporthe oryzae." Molecular Plant-Microbe Interactions® 30, no. 7 (2017): 517–30. http://dx.doi.org/10.1094/mpmi-11-16-0236-r.

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RNA interference (RNAi) is conserved in eukaryotic organisms, and it has been well studied in many animal and plant species and some fungal species, yet it is not well studied in fungal plant pathogens. In the rice blast fungus Magnaporthe oryzae, we examined small RNA (sRNA) and their biogenesis in the context of growth and pathogenicity. Through genetic and genomic analyses, we demonstrate that loss of a single gene encoding Dicer, RNA-dependent RNA polymerase, or Argonaute reduces sRNA levels. These three proteins are required for the biogenesis of sRNA-matching genome-wide regions (coding
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5

Yu, Yuen-Tsu N., Manuel Kleiner, and Gregory J. Velicer. "Spontaneous Reversions of an Evolutionary Trait Loss Reveal Regulators of a Small RNA That Controls Multicellular Development in Myxobacteria." Journal of Bacteriology 198, no. 23 (2016): 3142–51. http://dx.doi.org/10.1128/jb.00389-16.

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ABSTRACTLost traits can reevolve, but the probability of trait reversion depends partly on a trait's genetic complexity. Myxobacterial fruiting body development is a complex trait controlled by the small RNA (sRNA) Pxr, which blocks development under conditions of nutrient abundance. In developmentally proficient strains ofMyxococcus xanthus, starvation relaxes the inhibition by Pxr, thereby allowing development to proceed. In contrast, the lab-evolved strain OC does not develop because it fails to relay an early starvation signal that alleviates inhibition by Pxr. A descendant of OC, strain P
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6

Lambert, Marine, Sara Guellal, Jeffrey Ho, et al. "An Expanded Landscape of Unusually Short RNAs in 11 Samples from Six Eukaryotic Organisms." Non-Coding RNA 8, no. 3 (2022): 34. http://dx.doi.org/10.3390/ncrna8030034.

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Small RNA sequencing (sRNA-Seq) approaches unveiled sequences derived from longer non-coding RNAs, such as transfer RNA (tRNA) and ribosomal RNA (rRNA) fragments, known as tRFs and rRFs, respectively. However, rRNAs and RNAs shorter than 16 nt are often depleted from library preparations/sequencing analyses, although they may be functional. Here, we sought to obtain a complete repertoire of small RNAs by sequencing the total RNA from 11 samples of 6 different eukaryotic organisms, from yeasts to human, in an extended 8- to 30-nt window of RNA length. The 8- to 15-nt window essentially containe
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7

Grueso-Navarro, Elena, Leticia Rodríguez-Alcolado, Laura Arias-González, et al. "MicroRNAs in Plasma-Derived Extracellular Vesicles as Non-Invasive Biomarkers for Eosinophilic Esophagitis." International Journal of Molecular Sciences 26, no. 2 (2025): 639. https://doi.org/10.3390/ijms26020639.

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Eosinophilic esophagitis (EoE) is a chronic inflammatory esophageal disorder. The lack of non-invasive biomarkers currently results in dependency on endoscopy with biopsies for its diagnosis and monitoring. We aimed to identify potential non-invasive biomarkers using microRNAs (miRNAs) in plasma-derived extracellular vesicles (pEVs). This was a prospective single-center observational study of a discovery cohort of EoE patients (n = 26) with active disease (EoE.Basal) and after anti-inflammatory treatment (EoE.Post.tx) and control subjects (n = 16). Small-RNA-seq was performed to identify diffe
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8

Comstock, Laurie E. "Small RNAs Repress Expression of Polysaccharide Utilization Loci of Gut Bacteroides Species." Journal of Bacteriology 198, no. 18 (2016): 2396–98. http://dx.doi.org/10.1128/jb.00514-16.

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Bacteroidesspecies can metabolize numerous plant polysaccharides and host glycans present in the mammalian gut. The regulatory systems governing the induction of particular polysaccharide utilization loci when the cognate glycan is present are known, but how expression is repressed when a higher-priority glycan is present is largely unknown. In this issue of theJournal of Bacteriology, Cao et al. (J. Bacteriol. 198:2410–2418, 2016,http://dx.doi.org/10.1128/JB.00381-16) reveal a conserved mechanism inBacteroideswhereby antisense small RNAs (sRNA) repress expression of genes involved in utilizat
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9

Wibowo, Arif, Mas Tri Djoko Sunarno, Subagdja Subagdja, and Taufiq Hidayah. "KARAKTERISASI POPULASI IKAN PUTAK (Notopterus notopterus) MENGGUNAKAN ANALISIS KERAGAMAN FENOTIPIK DAN DAERAH 16 SRNA DNA MITOKONDRIA." Jurnal Penelitian Perikanan Indonesia 15, no. 1 (2017): 1. http://dx.doi.org/10.15578/jppi.15.1.2009.1-12.

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Penelitian ini dilakukan pada tahun 2006 di Sungai Ogan, Sungai Kelekar (anak Sungai Musi, Sumatera Selatan), perairan di Pulau Bangka serta Kota Bangun dan Tanah Ulu (Sungai Mahakam, Kalimantan Timur). Tujuannya adalah untuk menganalisis karakterisasi populasi ikan putak (Notopterus notopterus) dari berbagai habitat berdasarkan pada karakter fenotipik dan genetik serta untuk menentukan karakter morfologi sebagai pembeda utama, mendeterminasi jarak kemiripan antar populasi ikan putak dan untuk menganalisis apakah perbedaan morfologi antar populasi tersebut hanya sekedar fenotipik plastisity at
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10

Pannuri, Archana, Christopher A. Vakulskas, Tesfalem Zere, et al. "Circuitry Linking the Catabolite Repression and Csr Global Regulatory Systems of Escherichia coli." Journal of Bacteriology 198, no. 21 (2016): 3000–3015. http://dx.doi.org/10.1128/jb.00454-16.

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ABSTRACTCyclic AMP (cAMP) and the cAMP receptor protein (cAMP-CRP) and CsrA are the principal regulators of the catabolite repression and carbon storage global regulatory systems, respectively. cAMP-CRP controls the transcription of genes for carbohydrate metabolism and other processes in response to carbon nutritional status, while CsrA binds to diverse mRNAs and regulates translation, RNA stability, and/or transcription elongation. CsrA also binds to the regulatory small RNAs (sRNAs) CsrB and CsrC, which antagonize its activity. The BarA-UvrY two-component signal transduction system (TCS) di
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Cani, Alice, Carlotta Caterina Damanti, Federica Lovisa, et al. "Plasma S-Evs Mirnas in Pediatric B Lineage Leukemia: Transforming Factors of Bone Marrow Niche." Blood 142, Supplement 1 (2023): 4081. http://dx.doi.org/10.1182/blood-2023-188680.

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BACKGROUND Pediatric B-cell precursor acute lymphoblastic leukemia (BCP-ALL) and mature B-cell leukemia are characterized by dismal prognosis in case of relapse. Exosomes are crucial small extracellular vesicles (S-EVs) used by cells for intercellular communication, capable to modulate recipient cells through the release of small biological molecules (DNA, RNA, proteins). Tumor derived exosomes play an important role in leukemogenesis, disease progression, and organ invasion, due to their ability to induce molecular and functional changes within the bone marrow microenvironment (BME). Since li
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12

Mégroz, Marianne, Oded Kleifeld, Amy Wright, et al. "The RNA-Binding Chaperone Hfq Is an Important Global Regulator of Gene Expression in Pasteurella multocida and Plays a Crucial Role in Production of a Number of Virulence Factors, Including Hyaluronic Acid Capsule." Infection and Immunity 84, no. 5 (2016): 1361–70. http://dx.doi.org/10.1128/iai.00122-16.

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The Gram-negative bacteriumPasteurella multocidais the causative agent of a number of economically important animal diseases, including avian fowl cholera. NumerousP. multocidavirulence factors have been identified, including capsule, lipopolysaccharide (LPS), and filamentous hemagglutinin, but little is known about how the expression of these virulence factors is regulated. Hfq is an RNA-binding protein that facilitates riboregulation via interaction with small noncoding RNA (sRNA) molecules and their mRNA targets. Here, we show that aP. multocidahfqmutant produces significantly less hyaluron
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13

Prihandani, S. S., S. M. Noor, I. W. T. Wibawan, et al. "Polymerase chain reaction to confirm biochemically characterization method of Pasteurella multocida isolate from fatal cases of Septicaemia epizootica in Nusa Tenggara Timur." IOP Conference Series: Earth and Environmental Science 976, no. 1 (2022): 012046. http://dx.doi.org/10.1088/1755-1315/976/1/012046.

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Abstract Septicemia epizootica (SE) is a common fatal systemic disease in cattle and buffalo due to Pasteurella multocida serotype B:2 in South and Southeast Asia countries, including Indonesia. This infectious agent is generally considered an opportunistic pathogen and located in the nasopharynx of the animal. To support the disease’s diagnosis, an acceptable identification procedure must be established. This study was to confirm bovine P. multocida isolate that has been identified through biochemical approaches at the first step, with the Polymerase Chain Reaction method. The isolate was obt
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14

Xie, Xing, Chun Chen, and Xiong Fu. "Modulation Effects of Sargassum pallidum Extract on Hyperglycemia and Hyperlipidemia in Type 2 Diabetic Mice." Foods 12, no. 24 (2023): 4409. http://dx.doi.org/10.3390/foods12244409.

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The aim of this study was to investigate the antidiabetic effect of the extract from Sargassum pallidum (SPPE) on type 2 diabetes mellitus (T2DM) mice. SPPE treatment alleviated hyperglycemia, insulin resistance (IR), liver and pancreatic tissue damage, hyperlipidemia and hepatic oxidative stress resulting from T2DM. SPPE reversed phosphoenolpyruvate carboxylase (PEPCK) and hexokinase (HK) activities to improve gluconeogenesis and glycogen storage in the liver. Furthermore, SPPE modulated glucose metabolism by regulating the levels of mRNA expression involving the PI3K/Akt/FOXO1/G6pase/GLUT2 p
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15

Kuznetsova, Victoria E., Elena A. Matveeva, and Lyudmila A. Belovezhets. "THE PROSPECTS OF EASTERN SIBERIA Trichoderma FUNGI FOR PHYTOPATHO-GEN CONTROL." Siberian Journal of Life Sciences and Agriculture 16, no. 2 (2024): 213–38. http://dx.doi.org/10.12731/2658-6649-2024-16-2-831.

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Background. Throughout the world, the losses of agricultural products are associated with plant diseases, most often caused by microscopic fungi. Among the most effective methods to control these fungi is the use of various chemicals fungicides. However, the overutilization of such fungicides can induce negative implications. To address this challenge, alternatives methods of control using antagonist microbes are being developed. Of particular interest are fungi of the Trichoderma genus, which can be employed as biopreparations to protect crops against a number of diseases. Purpose. The study
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16

Lalaouna, D., S. Fochesato, M. Barakat, P. Ortet, and W. Achouak. "Multiple Transcription-Activating Sequences Regulate the RsmZ Regulatory Small RNA of Pseudomonas brassicacearum." Journal of Bacteriology 194, no. 18 (2012): 4888–93. http://dx.doi.org/10.1128/jb.00408-12.

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ABSTRACTThemutS-rpoSregion is known to be a highly polymorphic segment of the chromosome owing to horizontal gene transfer and evolutionary processes. InPseudomonas,mutS-fdxA-rsmZ-rpoSorganization is highly conserved, as well as the promoter region of the RsmZ small RNA (sRNA)-encoding gene. One exception to this conservation is inPseudomonas brassicacearum, where a 308-nucleotide (nt) sequence, predicted to form a hairpin structure in single-stranded DNA (ssDNA), is inserted between therpoSandrsmZgenes. Using MEME software, we identified nine consensus motifs in thersmZpromoter region of 16 s
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17

Bi, Y., K. Artola, T. Kurokura, T. Hytönen, and J. P. T. Valkonen. "First Report of Raspberry leaf blotch virus in Raspberries in Finland." Plant Disease 96, no. 8 (2012): 1231. http://dx.doi.org/10.1094/pdis-04-12-0368-pdn.

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Raspberry (Rubus idaeus L.) is a valuable and widely grown softfruit that is a host for 40 viruses and virus-like agents, of which many are not characterized at the molecular level. Recently, Raspberry leaf blotch virus (RLBV, putative emaravirus species) was described from raspberries (cv. Glen Ample) in the United Kingdom and Serbia. Plants displayed conspicuous yellow blotches on leaves and abnormal development of leaf hairs in the corresponding areas of the abaxial side (3). Similar symptoms were observed in ‘Glen Ample’ grown in protective plastic tunnels and open fields in the main berry
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18

Xie, Yanshe, Guangbin Liu, Xupeng Zang, et al. "Differential Expression Pattern of Goat Uterine Fluids Extracellular Vesicles miRNAs during Peri-Implantation." Cells 10, no. 9 (2021): 2308. http://dx.doi.org/10.3390/cells10092308.

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Early pregnancy failure occurs when a mature embryo attaches to an unreceptive endometrium. During the formation of a receptive endometrium, extracellular vesicles (EVs) of the uterine fluids (UFs) deliver regulatory molecules such as small RNAs to mediate intrauterine communication between the embryo and the endometrium. However, profiling of small RNAs in goat UFs’ EVs during pregnancy recognition (day 16) has not been carried out. In this study, EVs were isolated from UFs on day 16 of the estrous cycle or gestation. They were isolated by Optiprep™ Density G radient (ODG) and verified by tra
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Wang, Qingyi, Zhigang Zhao, Xiaotuo Zhang, et al. "Identifying microRNAs and Their Editing Sites in Macaca mulatta." Cells 8, no. 7 (2019): 682. http://dx.doi.org/10.3390/cells8070682.

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MicroRNAs (miRNAs) are small non-coding RNAs that are critical in post-transcriptional regulation. Macaca mulatta is an important nonhuman primate that is often used in basic and translational researches. However, the annotation of miRNAs in Macaca mulatta is far from complete, and there are no reports of miRNA editing events in Macaca mulatta, although editing may affect the biogenesis or functions of the miRNAs. To improve miRNA annotation and to reveal editing events of miRNAs in Macaca mulatta, we generated 12 small RNA profiles from eight tissues and performed comprehensive analysis of th
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Zhao, Xinchi, Guiyan Yang, Xiaoqiang Liu, Zhongdong Yu, and Shaobing Peng. "Integrated Analysis of Seed microRNA and mRNA Transcriptome Reveals Important Functional Genes and microRNA-Targets in the Process of Walnut (Juglans regia) Seed Oil Accumulation." International Journal of Molecular Sciences 21, no. 23 (2020): 9093. http://dx.doi.org/10.3390/ijms21239093.

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Walnut (Juglans regia) is known as a promising woody oil crop with abundant polyunsaturated fatty acids in its kernel. However, the regulation mechanism of walnut oil accumulation and fatty acid metabolism is still poorly understood, which restricted the breeding and genetic improvement of high-quality oil-bearing walnuts. To reveal the molecular mechanism of walnut oil accumulation, considering the potential regulation of microRNA (miRNA) in seed development, in this study, the oil content of walnut kernel on the 80th, 100th and 120th day after flowering (DAF) was tested and the corresponding
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21

Wang, Qiaoxin, Xiaohui Li, Hang Sha, Xiangzhong Luo, Guiwei Zou, and Hongwei Liang. "Identification of microRNAs in Silver Carp (Hypophthalmichthys molitrix) Response to Hypoxia Stress." Animals 11, no. 10 (2021): 2917. http://dx.doi.org/10.3390/ani11102917.

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Hypoxia is one of the serious stresses in fish culture, which can lead to physical and morphological changes, and cause injury and even death to fish. Silver carp (Hypophthalmichthys molitrix) is an important economic fish and widely distributed in China. MicroRNA is a kind of endogenous non-coding single-stranded small RNA, which is involved in cell development, and immune response and gene expression regulation. In this study, silver carp were kept in the closed containers for hypoxia treatment by spontaneous oxygen consumption. The samples of heart, brain, liver and gill were collected, and
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22

Bryant, Voris, Modoluwamu Idowu, Thomas H. Terrill, et al. "PSXI-28 Effect of pre-slaughter transport stress on carcass weight, rumen fermentation and bacterial community of growing goats." Journal of Animal Science 99, Supplement_3 (2021): 484. http://dx.doi.org/10.1093/jas/skab235.854.

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Abstract This study evaluated the effects of pre-slaughter transport stress on the rumen fermentation and bacterial community of growing meat goats. Fifty-four male Spanish growing meat goats (28.9 ± 4.5) were stratified by body weight and randomly assigned into three treatment groups (n = 18 per treatment; 1) No transport stress (CON), 2) Transport stress for 30 mins (LS), and 3) Transport stress for 3 h (HS). Blood samples were collected before and after transport to estimate plasma cortisol concentrations. At the end of the experiment, the goats were slaughtered to determine the carcass wei
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23

Phinney, Thomson R., Ali Alwadei, Gabriel Gabriel Wajnberg, et al. "Abstract B021: tRNA fragments extracted from patient plasma extra cellular vesicles display potential as biomarkers for high-grade glioma." Clinical Cancer Research 30, no. 21_Supplement (2024): B021. http://dx.doi.org/10.1158/1557-3265.liqbiop24-b021.

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Abstract Transfer RNA (tRNA) are non-coding RNAs that bring amino acids to ribosomes during translation. Recent studies have shown that cleaved fragments derived from tRNA (tRFs) serve regulatory roles in epigenetics, signaling, transcription, and translation. tRFs can be released from cells and can be found in extracellular vesicles to perform some aspects of paracrine signaling. Utilizing high throughput small RNA sequencing, we have identified over 750 uniquely or multi-mapped (6.9% of total sRNA mapped reads) tRFs in extracellular vesicles of high-grade glioma (IDH-WT) patient plasma. By p
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24

Colbert, Lauren Elizabeth, Andrea Delgado Medrano, Rebecca A. Previs, et al. "Association of changes in vaginal microbiome with oligoclonal T-cell expansion and early response to chemoradiation for cervical cancer." Journal of Clinical Oncology 36, no. 5_suppl (2018): 8. http://dx.doi.org/10.1200/jco.2018.36.5_suppl.8.

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8 Background: The composition of the vaginal microbiome has been shown to affect clearance of HPV virus and transformation to invasive cancer. Clinical studies correlating the vaginal microbiome with immune activation and response to cancer treatment are lacking. We profiled intratumoral T-cell clonality during radiation therapy and correlated it with the diversity of the vaginal flora. Methods: Thirty patients with newly diagnosed locally advanced cervical cancer were enrolled on a prospective study to characterize changes in the cervical microbiome during chemoradiation. Cervical samples wer
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25

Kopcho, Steven, Marina McDew-White, Wasifa Naushad, Mahesh Mohan, and Chioma M. Okeoma. "SIV Infection Regulates Compartmentalization of Circulating Blood Plasma miRNAs within Extracellular Vesicles (EVs) and Extracellular Condensates (ECs) and Decreases EV-Associated miRNA-128." Viruses 15, no. 3 (2023): 622. http://dx.doi.org/10.3390/v15030622.

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Background: This is Manuscript 1 of a two-part Manuscript of the same series. Here, we present findings from our first set of studies on the abundance and compartmentalization of blood plasma extracellular microRNAs (exmiRNAs) into extracellular particles, including blood plasma extracellular vesicles (EVs) and extracellular condensates (ECs) in the setting of untreated HIV/SIV infection. The goals of the study presented in this Manuscript 1 are to (i) assess the abundance and compartmentalization of exmiRNAs in EVs versus ECs in the healthy uninfected state, and (ii) evaluate how SIV infectio
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Vaknin, Yakir, Falk Hillmann, Rossana Iannitti, et al. "Identification and Characterization of a Novel Aspergillus fumigatus Rhomboid Family Putative Protease, RbdA, Involved in Hypoxia Sensing and Virulence." Infection and Immunity 84, no. 6 (2016): 1866–78. http://dx.doi.org/10.1128/iai.00011-16.

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Aspergillus fumigatusis the most common pathogenic mold infecting humans and a significant cause of morbidity and mortality in immunocompromised patients. In invasive pulmonary aspergillosis,A. fumigatusspores are inhaled into the lungs, undergoing germination and invasive hyphal growth. The fungus occludes and disrupts the blood vessels, leading to hypoxia and eventual tissue necrosis. The ability of this mold to adapt to hypoxia is regulated in part by the sterol regulatory element binding protein (SREBP) SrbA and the DscA to DscD Golgi E3 ligase complex critical for SREBP activation by prot
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Spanka, Daniel-Timon, and Gabriele Klug. "Maturation of UTR-Derived sRNAs Is Modulated during Adaptation to Different Growth Conditions." International Journal of Molecular Sciences 22, no. 22 (2021): 12260. http://dx.doi.org/10.3390/ijms222212260.

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Small regulatory RNAs play a major role in bacterial gene regulation by binding their target mRNAs, which mostly influences the stability or translation of the target. Expression levels of sRNAs are often regulated by their own promoters, but recent reports have highlighted the presence and importance of sRNAs that are derived from mRNA 3′ untranslated regions (UTRs). In this study, we investigated the maturation of 5′ and 3′ UTR-derived sRNAs on a global scale in the facultative phototrophic alphaproteobacterium Rhodobacter sphaeroides. Including some already known UTR-derived sRNAs like UpsM
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Li, Maoqing, Zichen Gao, Zewen He, et al. "Effects of Silicone Rubber on Rheological Properties and Aging Characteristics of Asphalt Binder." Polymers 16, no. 13 (2024): 1903. http://dx.doi.org/10.3390/polym16131903.

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Silicone rubber (SR) is a kind of polymer insulation material with excellent performance. With the service life of silicone rubber products reaching the limit, how to dispose of waste silicone rubber is an urgent problem to be solved. In this paper, silicone rubber-modified asphalt binder (SRMA) was prepared by SR and 90# base asphalt binder. The simulated short-term aging and long-term aging tests of SRMA were carried out using the thin film oven aging test (TFOT) and pressure aging vessel test (PAV). The rotary viscosity test and dynamic shear rheological test (DSR) were applied to the rheol
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Doder, Filip, Ines Blažeković, and Aleksandar Ovuka. "Kardiogeni šok u novorođenčeta kao posljedica supraventrikularne tahikardije." Medicina Fluminensis 58, no. 1 (2022): 72–75. http://dx.doi.org/10.21860/medflum2022_271162.

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Cilj: prikazati slučaj kardiogenog šoka koji se razvio kao posljedica dugotrajne atake supraventrikularne tahikardije u novorođenčeta u dobi od 16 dana. Prikaz slučaja: Muško novorođenče u dobi od 16 dana dovezeno je na hitni prijam Klinike za pedijatriju zbog odbijanja obroka te lošeg općeg stanja. Pri prijamu je blijedo, tahikardno, dispnoično, hladnih okrajina, produženog vremena rekapilarizacije, normotenzivno s oslabljenim perifernim arterijskim pulsacijama. Fizikalnim pregledom uočene su nad plućima bazalno krepitacije uz ritmičnu akciju srca frekvencije oko 250/min. Jetra se palpirala z
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Tian-ya, Li, Wu Xian-xin, Xu Xiao-feng, Wang Wan-lin, and Cao Yuan-yin. "Postulation of seedling stem rust resistance genes of Yunnan wheat cultivars in China." Plant Protection Science 52, No. 4 (2016): 242–49. http://dx.doi.org/10.17221/137/2015-pps.

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To determine stem rust resistance genes of wheat varieties in Yunnan province, 11 Chinese strains of Puccinia graminis f.sp. tritici with different virulence and 1 artificial mutant strain were used in 110 wheat varieties. The results indicated that among the 45 Sr genes, Sr31, Sr5, SrTmp, Sr30, Sr36, Sr8a, Sr11, Sr24, Sr29, Sr34, Sr9e, Sr26, Sr38, Sr47, and SrTt3 were characterised in 55 wheat varieties singly or in combination. Sr5, Sr31, SrTmp, Sr36, and Sr30 were contained in 17, 16, 5, 5, and 3 cultivars, respectively. Six cultivars contained Sr24 and/or Sr34. Three plant materials likely
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Schullian, Peter, Gregor Laimer, Edward Johnston, et al. "Reliability of Stereotactic Radiofrequency Ablation (SRFA) for Malignant Liver Tumors: Novice versus Experienced Operators." Biology 12, no. 2 (2023): 175. http://dx.doi.org/10.3390/biology12020175.

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Purpose: To compare the results of a novice with those of experienced interventional radiologists (IRs) for stereotactic radiofrequency ablation (SRFA) of malignant liver tumors in terms of safety, technical success, and local tumor control. Methods: A database, including all SRFA procedures performed in a single center between January 2011 and December 2018 was retrospectively analyzed. A total of 39 ablation sessions performed by a novice IR were compared to the results of three more experienced IRs. Comparative SRFA sessions were selected using propensity score matching considering tumor ty
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Chen, Qing-Yun, and Zai-Ming Qiu. "Studies on fluoroalkylation and fluoroalkoxylation. Part 16. Reactions of fluoroalkyl iodides with some nucleophiles by SRN1 mechanism." Journal of Fluorine Chemistry 35, no. 2 (1987): 343–57. http://dx.doi.org/10.1016/s0022-1139(00)85017-4.

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Guo, Zhi Zhuang, and Xiao Ling Wang. "Research on Very Low Resolution Face Image Reconstruction." Applied Mechanics and Materials 687-691 (November 2014): 3747–50. http://dx.doi.org/10.4028/www.scientific.net/amm.687-691.3747.

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The resolution of the face image in video may lower than 16*16 in environmental such as ultra long distance, poor illumination and so on, with the very low resolution (VLR) face image the existing face super-resolution (SR) methods do not perform well. In this paper, we proposes a new algorithms by learning the relationship between high-resolution (HR) image space and the VLR image space for face SR.A new constrain, new data constrain are design for reconstruct HR face image form VLR face image. The Experiment results show that the proposed method can recover a clear face image from the VLR fa
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Diallo, Idrissa, Jeffrey Ho, Marine Lambert, et al. "A tRNA-derived fragment present in E. coli OMVs regulates host cell gene expression and proliferation." PLOS Pathogens 18, no. 9 (2022): e1010827. http://dx.doi.org/10.1371/journal.ppat.1010827.

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RNA-sequencing has led to a spectacular increase in the repertoire of bacterial sRNAs and improved our understanding of their biological functions. Bacterial sRNAs have also been found in outer membrane vesicles (OMVs), raising questions about their potential involvement in bacteria-host relationship, but few studies have documented this issue. Recent RNA-Sequencing analyses of bacterial RNA unveiled the existence of abundant very small RNAs (vsRNAs) shorter than 16 nt. These especially include tRNA fragments (tRFs) that are selectively loaded in OMVs and are predicted to target host mRNAs. He
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KOSTERIN, OLEG E., and TOM KOMPIER. "Coeliccia rolandorum sp. nov. from eastern Cambodia and southern Vietnam, the eastern relative of C. kazukoae Asahina, 1984 (Odonata: Platycnemididae)." Zootaxa 4341, no. 4 (2017): 509. http://dx.doi.org/10.11646/zootaxa.4341.4.4.

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Coeliccia rolandorum sp. nov. is described from the eastern Cambodia (holotype: Cambodia, Mondulkiri Province, Buu Sraa Waterfall environs, 12°34' N 107°24' E, ~780 m a.s.l., 16 June 2014, RMNH) and southern and central Vietnam. It is related to C. kazukoae, which is known from the Cardamom and Sankamphaeng Mts., and replaces it in eastern Indochina. New distributional data on C. kazukoae are presented and its characters are discussed with respect to their change with age.
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Cao, Yanlu, Konrad U. Förstner, Jörg Vogel, and C. Jeffrey Smith. "cis-Encoded Small RNAs, a Conserved Mechanism for Repression of Polysaccharide Utilization in Bacteroides." Journal of Bacteriology 198, no. 18 (2016): 2410–18. http://dx.doi.org/10.1128/jb.00381-16.

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ABSTRACTBacteroidesis a major component of the human gut microbiota which has a broad impact on the development and physiology of its host and a potential role in a wide range of disease syndromes. The predominance of this genus is due in large part to expansion of paralogous gene clusters, termedpolysaccharideutilizationloci (PULs), dedicated to the uptake and catabolism of host-derived and dietary polysaccharides. The nutritive value and availability of polysaccharides in the gut vary greatly; thus, their utilization is hierarchical and strictly controlled. A typical PUL includes regulatory
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Hobbs, Errett C., Jillian L. Astarita, and Gisela Storz. "Small RNAs and Small Proteins Involved in Resistance to Cell Envelope Stress and Acid Shock in Escherichia coli: Analysis of a Bar-Coded Mutant Collection." Journal of Bacteriology 192, no. 1 (2009): 59–67. http://dx.doi.org/10.1128/jb.00873-09.

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ABSTRACT More than 80 small regulatory RNAs (sRNAs) and 60 proteins of 16 to 50 amino acids (small proteins) are encoded in the E scherichia coli genome. The vast majority of the corresponding genes have no known function. We screened 125 DNA bar-coded mutants to identify novel cell envelope stress and acute acid shock phenotypes associated with deletions of genes coding for sRNAs and small proteins. Nine deletion mutants (ssrA, micA, ybaM, ryeF, yqcG, sroH, ybhT, yobF, and glmY) were sensitive to cell envelope stress and two were resistant (rybB and blr). Deletion mutants of genes coding for
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Ejigu, Neway, Negussie Sarbecha, Kenbon Seyoum, et al. "Prevalence of low birth weight and associated factors in Ethiopia: An umbrella review of systematic review and meta-analyses." PLOS Global Public Health 5, no. 5 (2025): e0004556. https://doi.org/10.1371/journal.pgph.0004556.

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Low birth weight (LBW) is one of the major causes of neonatal mortality and morbidity in low and middle-income countries (LMICs). Despite the goal of reducing newborn morbidity and mortality by 2030, low-income countries, including Ethiopia, still confront major challenges. Although various systematic reviews and meta-analyses (SRMA) have been conducted on LBW in Ethiopia, there is notable variation among their findings. This umbrella review aimed to consolidate inconsistent findings into a single summary estimate, providing a robust synthesis of evidence from systematic reviews and meta-analy
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SELIMOVIĆ, Sead. "ROGATICA OD 1991. DO 2013. GODINE: PROMJENE U ETNIČKOJ STRUKTURI STANOVNIŠTVA POD UTJECAJEM RATA PROTIV BOSNE I HERCEGOVINE." Bastina sjeveroistocne Bosne 15, no. 15 (2023): 69–107. http://dx.doi.org/10.55258/issn.2232-7665.2023.15.69.

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Prije agresije na Republiku Bosnu i Hercegovinu, u Rogatici su zajedno živjeli Bošnjaci, Srbi, Hrvati, Jugoslaveni i Ostali. U ukupnom stanovništvu općine Rogatica bilo je 60,10% Bošnjaka, 38,18% Srba, 0,09% Hrvata, 0,85% Jugoslavena i 0,79% Ostalih. U gradu Rogatica, Bošnjaci su činili 63,72% ukupnog stanovništva, Srbi 33,32%, Hrvati 0,18%, Jugoslaveni 1,57% a Ostali 1,21%. Stanovnici ovoga područja bili su naviknuti na zajedništvo kroz obrazovni, privredni, kulturni i sportski život. Rukovodstvo Srpske Demokratske Stranke (SDS) težilo je da provede u djelo planove Slobodana Miloševića i Srps
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Zhao, Jing, Xiao Jun Xu, Qiang Zhan, Xuan Li, Shu Li Liu, and Tian Guo Li. "Study on Adsorption Characteristics of Klebsiella Biological Adsorbent to Leaded Wastewater." Advanced Materials Research 926-930 (May 2014): 4353–56. http://dx.doi.org/10.4028/www.scientific.net/amr.926-930.4353.

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A lead-resistant strains was isolated from activated sludge of the sewage treatment plant in Chenggong County, Kunming, which was identified as Klebsiella by 16 SrDNA. The wet microbial cells were used as adsorbent, we studied adsorption properties for water Pb (II) ions in water of sorbent. The results showed that when the adsorbent treated the waste water with Pb (II) ions, the pH was 5, the time was 60min, the temperature was 30°C, the equilibrium adsorption amount was 73.45mg/g; Adsorbent’s adsorption of Pb (II) ions can better fit Langmuir isotherm model. The FT-IR result presented that t
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Vargas-Arana, Gabriel, Claudia Merino-Zegarra, Marcos Riquelme-Penaherrera, et al. "Antihyperlipidemic and Antioxidant Capacities, Nutritional Analysis and UHPLC-PDA-MS Characterization of Cocona Fruits (Solanum sessiliflorum Dunal) from the Peruvian Amazon." Antioxidants 10, no. 10 (2021): 1566. http://dx.doi.org/10.3390/antiox10101566.

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Cocona fruits are a popular food and medicinal fruit used mainly in the Amazon and several countries of South America for the preparation of several food products such as drinks, jams and milk shakes. In this study five ecotypes of cocona native to Peru have been studied regarding their nutritional and antioxidants values plus antihyperlipidemic activities. Seventy bioactive compounds have been detected in Peruvian cocona ecotypes including several phenolic acids, aminoacids and flavonoids; of those six were spermidines, (peaks 1, 2, 25, 26, 38 and 39), thirteen were aminoacids, (peaks 3–9, 11
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Muñoz Cajiao, Carlos Alberto. "Aislamiento y caracterización molecular de bacterias fermentadoras tipo zymomonas mobilis, mediante la detección del gen pdc y secuenciación de los genes 16srdna y rpob." Ingeniería Química y Desarrollo 1, no. 1 (2021): 23–28. http://dx.doi.org/10.53591/iqd.v1i1.1255.

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El presente trabajo se basa en la identificación y caracterización molecular de los genes pdc (piruvato decarboxilasa), impulsores en la producción de alcohol etílico que se encuentran en las cepas aisladas de las bacterias Zymomonas mobilis, obtenidas del agave, que es un lixiviado de la planta cabuya, de la Provincia del Tungurahua, Ecuador. Para este fin, se hizo uso de la secuenciación de los genes 16 SrDNA y rpoB. Para la caracterización de las cepas aisladas mediante técnicas de cultivo, se empleó iniciadores universales del gen 16S del ARN ribosómico (16S rRNAF/R) y del gen de la ARN po
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Yu, Chunxiao, Ryan McClure, Kathleen Nudel, Nadine Daou, and Caroline Attardo Genco. "Characterization of the Neisseria gonorrhoeae Iron and Fur Regulatory Network." Journal of Bacteriology 198, no. 16 (2016): 2180–91. http://dx.doi.org/10.1128/jb.00166-16.

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ABSTRACTTheNeisseria gonorrhoeaeferricuptakeregulator (Fur) protein controls expression of iron homeostasis genes in response to intracellular iron levels. In this study, using transcriptome sequencing (RNA-seq) analysis of anN. gonorrhoeaefurstrain, we defined the gonococcal Fur and iron regulons and characterized Fur-controlled expression of an ArsR-like DNA binding protein. We observed that 158 genes (8% of the genome) showed differential expression in response to iron in anN. gonorrhoeaewild-type orfurstrain, while 54 genes exhibited differential expression in response to Fur. The Fur regu
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44

Bertelli, Fanny, Carey Meredith Suehs, Jean Pierre Mallet, et al. "Apnoea–hypopnoea indices determined via continuous positive airway pressure (AHI-CPAPflow) versus those determined by polysomnography (AHI-PSGgold): a protocol for a systematic review and meta-analysis." BMJ Open 11, no. 5 (2021): e044499. http://dx.doi.org/10.1136/bmjopen-2020-044499.

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Introduction To date, continuous positive airway pressure (CPAP) remains the cornerstone of obstructive sleep apnoea treatment. CPAP data describing residual sleep-disordered breathing events (ie, the CPAP-measured apnoea–hypopnoea indices (AHI-CPAPflow)) is difficult to interpret because it is an entirely different metric than the polysomnography (PSG) measured AHI gold standard (AHI-PSGgold). Moreover, manufacturer definitions for apnoea and hypopnoea are not only different from those recommended for PSG scoring, but also different between manufacturers. In the context of CPAP initiation and
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Davies, Adam, Monaghan W. Patrick, and Hogan Gerard. "Implementing an educational program to increase preoperative screening for obstructive sleep apnea using the stop-bang questionnaire." International Journal of Advanced Nursing Studies 5, no. 1 (2016): 56. http://dx.doi.org/10.14419/ijans.v5i1.5551.

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<p><strong>Background:</strong> Obstructive sleep apnea (OSA) is a potentially fatal disease process that has been linked to higher rates of morbidity and mortality as well as increased perioperative complications. OSA is characterized by repetitive pauses in breathing during sleep. Greater than 92% of women and 82% of men who are plagued by moderate to severe sleep apnea are undiagnosed and may go unrecognized in the perioperative setting. The gap between a high prevalence of undiagnosed OSA in the adult population and the low level of clinical recognition has been well-docu
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Goldman, Joshua, and Jennifer Beck. "YOUTH MARATHON TRAINING: INJURY EPIDEMIOLOGY & RISK FACTORS." Orthopaedic Journal of Sports Medicine 7, no. 3_suppl (2019): 2325967119S0006. http://dx.doi.org/10.1177/2325967119s00063.

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Background Youth participation in long distance running has increased yet little data exist about the injury patterns and safety of such activity. There are inherent training challenges in youth athletes that theoretically increase their risk of injury relative to their adult counterparts. These include their ongoing neuromuscular development and evolving gait, vulnerable growth open plates, increasing limb length that outpaces muscle growth, and reduced heat tolerance. Students Run LA (SRLA), now in its 30th year of operation, is a non-profit organization that mentors youth during an eight-mo
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Hajissa, Khalid, Md Asiful Islam, Siti Asma Hassan, Abdul Rahman Zaidah, Nabilah Ismail, and Zeehaida Mohamed. "Seroprevalence of SARS-CoV-2 Antibodies in Africa: A Systematic Review and Meta-Analysis." International Journal of Environmental Research and Public Health 19, no. 12 (2022): 7257. http://dx.doi.org/10.3390/ijerph19127257.

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A reliable estimate of SARS-CoV-2-specific antibodies is increasingly important to track the spread of infection and define the true burden of the ongoing COVID-19 pandemic. A systematic review and a meta-analysis were conducted with the objective of estimating the seroprevalence of SARS-CoV-2 infection in Africa. A systematic search of the PubMed, Scopus, Web of Science and Google Scholar electronic databases was conducted. Thirty-five eligible studies were included. Using meta-analysis of proportions, the overall seroprevalence of anti-SARS-CoV-2 antibodies was calculated as 16% (95% CI 13.1
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Dahal, Keshav, Khalil Kane, Fathey Sarhan, Bernard Grodzinski, and Norman P. A. Hüner. "Cold acclimation inhibits CO2-dependent stimulation of photosynthesis in spring wheat and spring rye." Botany 90, no. 6 (2012): 433–44. http://dx.doi.org/10.1139/b2012-007.

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We assessed the effects of short-term elevated CO2 on the light-saturated rates of photosynthesis (Asat) of spring (‘SR4A’, ‘Katepwa’) and winter (‘Musketeer’, ‘Norstar’) wheat ( Triticum aestivum L.) and rye ( Secale cereale L.) cultivars grown at ambient CO2 (380 µmol C·mol–1) at either 20/16 °C (nonacclimated, NA) or 5/5 °C (cold acclimated, CA). In spring wheat–rye, cold acclimation decreased CO2-stimulation of Asat by 45%–60% relative to NA controls following a short-term (80 h) shift of plants from ambient to elevated CO2 (700 µmol C·mol–1). In contrast, in winter wheat–rye, cold acclima
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Zhan, Chengxiu, Long Chen, Dandan Guo, et al. "An Intestinal Symbiotic Bacterial Strain of Oscheius chongmingensis Modulates Host Viability at Both Global and Post-Transcriptional Levels." International Journal of Molecular Sciences 23, no. 23 (2022): 14692. http://dx.doi.org/10.3390/ijms232314692.

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A rhabditid entomopathogenic nematode (EPN), Oscheius chongmingensis, has a stable symbiotic relationship with the bacterial strain Serratia nematodiphila S1 harbored in its intestines and drastically reduced viability when associated with a non-native strain (186) of the same bacterial species. This nematode is thus a good model for understanding the molecular mechanisms and interactions involved between a nematode host and a member of its intestinal microbiome. Transcriptome analysis and RNA-seq data indicated that expression levels of the majority (8797, 87.59%) of mRNAs in the non-native c
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Bojanovič, Klara, Isotta D'Arrigo, and Katherine S. Long. "Global Transcriptional Responses to Osmotic, Oxidative, and Imipenem Stress Conditions in Pseudomonas putida." Applied and Environmental Microbiology 83, no. 7 (2017). http://dx.doi.org/10.1128/aem.03236-16.

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ABSTRACTBacteria cope with and adapt to stress by modulating gene expression in response to specific environmental cues. In this study, the transcriptional response ofPseudomonas putidaKT2440 to osmotic, oxidative, and imipenem stress conditions at two time points was investigated via identification of differentially expressed mRNAs and small RNAs (sRNAs). A total of 440 sRNA transcripts were detected, of which 10% correspond to previously annotated sRNAs, 40% to novel intergenic transcripts, and 50% to novel transcripts antisense to annotated genes. Each stress elicits a unique response as fa
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