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Dissertations / Theses on the topic '16S rDNA gene sequencing'

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Published: 2 June 2025

Last updated: 31 July 2025

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1

Ziegler, Katie. "Phylogenetic Analysis of a Group of Enteric Bacteria Based on 16S rDNA Gene Sequencing." Miami University Honors Theses / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1111684418.

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2

Torres, Adalgisa Ribeiro. "Diversidade genética de enterobactérias endofíticas de diferentes hospedeiros e colonização de Catharantus roseus por endófitos expressando o gene gfp." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-03062005-152149/.

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Bactérias endofíticas são aquelas que habitam o interior de tecidos vegetais, sem causar dano aparente aos mesmos, além de desempenhar funções importantes no processo de adaptação das plantas. Especial interesse tem sido dado a tais bactérias devido ao seu potencial no controle biológico. Por isso, é muito importante estudar a diversidade genética de endófitos, além de avaliar o impacto da introdução de endófitos geneticamente modificados no ambiente. Estudos vêm sendo feitos nesse sentido, mas não com bactérias endofíticas da família Enterobacteriaceae. Desta forma, o presente trabalho tev

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3

Fogler, Kendall Wilson. "Effect of Soil Amendments from Antibiotic Treated Cows on Antibiotic Resistant Bacteria and Genes Recovered from the Surfaces of Lettuce and Radishes: Field Study." Thesis, Virginia Tech, 2018. http://hdl.handle.net/10919/92587.

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Cattle are commonly treated with antibiotics that may survive digestion and promote antibiotic resistance when manure or composted manure is used as a soil amendment for crop production. This study was conducted to determine the effects of antibiotic administration and soil amendment practices on microbial diversity and antibiotic resistance of bacteria recovered from the surfaces of lettuce and radishes grown using recommended application rates. Vegetables were planted in field plots amended with raw manure from antibiotic-treated dairy cows, composted-manure from cows with different historie

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4

ARAÚJO, Livia Caroline Alexandre de. "Caracterização molecular das linhagens de Zymomonas mobilis da coleção de micro-organismos UFPEDA." Universidade Federal de Pernambuco, 2014. https://repositorio.ufpe.br/handle/123456789/17179.

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Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2016-06-29T12:07:11Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Dissertação Livia Araujo.pdf: 1983609 bytes, checksum: cbba526737c10f6743b3fd015372721a (MD5)<br>Made available in DSpace on 2016-06-29T12:07:11Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Dissertação Livia Araujo.pdf: 1983609 bytes, checksum: cbba526737c10f6743b3fd015372721a (MD5) Previous issue date: 2014-02-20<br>CAPEs<br>Zymomonas mobilis vem de

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5

Tam, Man-wah. "Identification of bacterial pathogens by 16S ribosomal RNA gene sequencing." Click to view the E-thesis via HKUTO, 2002. http://sunzi.lib.hku.hk/hkuto/record/B31970783.

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6

Li, Kwan-hing. "Identification of bacterial pathogens by 16S ribosomal RNA gene sequencing." Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B31971982.

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7

Li, Kwan-hing, and 李群卿. "Identification of bacterial pathogens by 16S ribosomal RNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31971982.

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8

譚文華 and Man-wah Tam. "Identification of bacterial pathogens by 16S ribosomal RNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B31970783.

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9

Netto, Osmar Vaz de Carvalho. "Identificação de bactérias contaminantes de fermento de cachaça por seqüenciamento do gene 16S rDNA." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-08082007-164157/.

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A cachaça é uma bebida típica brasileira produzida a partir da destilação do caldo de canade- açúcar fermentado principalmente por Saccharomyces cerevisiae. Grande parte da produção nacional é artesanal, e não há uma preocupação por parte dos produtores quanto ao controle microbiológico da fermentação. Este trabalho objetivou caracterizar a comunidade bacteriana contaminante do fermento utilizado na produção de cachaça. Foram coletadas quatro amostras em um alambique artesanal. A primeira (NA) foi coletada um ano anterior às outras três e utilizada como controle. As restantes foram coletadas a

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10

鄧莉莉 and Lee-lee Jade Teng. "Identification of thermo-tolerant campylobacter fetus by 16S ribosomalRNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B3196994X.

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11

Perim, Júlia Elídia de Lima. "Diversidade microbiana envolvida na ciclagem do nitrogênio em solos de cultivo de cana-de-açúcar no estado de São Paulo." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-28112016-175102/.

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O Brasil é o maior produtor mundial de cana-de-açúcar, sendo o Estado de São Paulo responsável por mais de 50% do total desta produção. Esta cultura tem um enorme impacto sobre a agricultura brasileira e devido a isto, uma melhor compreensão da composição da comunidade microbiana relacionada a ciclagem do nitrogênio (N) nestes solos é de grande importância para o aprimoramento no cultivo da cana-de-açúcar. No entanto, pouco se sabe sobre a relação entre grupos microbianos e essa cultura. Este trabalho visou determinar variações nas comunidades microbianas que participam das transformações do N

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12

招紹裘 and Siu-kau Chiu. "Granulicatella, abiotrophia, and gemella bacteremia characterized by 16S ribosomal RNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B3197045X.

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13

Teng, Lee-lee Jade. "Identification of thermo-tolerant campylobacter fetus by 16S ribosomal RNA gene sequencing." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B2334023X.

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14

Chiu, Siu-kau. "Granulicatella, abiotrophia, and gemella bacteremia characterized by 16S ribosomal RNA gene sequencing." Hong Kong : University of Hong Kong, 2002. http://sunzi.lib.hku.hk/hkuto/record.jsp?B25151605.

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15

Lostak, Tracy Karon. "Genetic variation in the 16s mitochondrial rDNA gene from Texas and Oklahoma populations of Amblyomma maculatum." [College Station, Tex. : Texas A&M University, 2008. http://hdl.handle.net/1969.1/ETD-TAMU-2957.

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16

Lesack, Kyle. "Nomenclature errors in public 16s rDNA gene databases : strategies to improve the accuracy of sequence annotations." Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/62456.

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Obtaining an accurate representation of the microorganisms present in microbial ecosystems presents a considerable challenge. Microbial communities are typically highly complex, and may consist of a variety of differentially abundant bacteria, archaea, and microbial eukaryotes. The targeted sequencing of the 16S rDNA gene has become a standard method for profiling membership and biodiversity of microbial communities, as the bacterial and archaeal community members may be profiled directly, without any intermediate culturing steps. These studies rely upon specialized 16S rDNA gene reference dat

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17

Al, Masalma Mouhamad. "Molecular and cultural analysis of the bacterial flora associated with brain abscesses." Thesis, Aix-Marseille 2, 2011. http://www.theses.fr/2011AIX20663/document.

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Les abcès cérébraux sont des infections potentiellement mortelles, entraînant souvent des séquelles graves. La prise en charge médicale en reste empirique en raison d’un manque de connaissance approfondie des microorganismes responsables de cette condition. Dans la plupart des laboratoires microbiologiques, le diagnostic d’abcès cérébral est basé sur la culture du pus recueilli chirurgicalement. Malheureusement, cette procédure a de nombreuses limites et ne permet l’identification que d’une petite partie de la population microbienne en cause. L’amplification par PCR et le séquençage du gène co

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18

陳賢良 and Yin-leung Chan. "Identification of bacteria with ambiguous biochemical profiles by 16S ribosomal RNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B3197028X.

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19

Chan, Yin-leung. "Identification of bacteria with ambiguous biochemical profiles by 16S ribosomal RNA gene sequencing." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk:8888/cgi-bin/hkuto%5Ftoc%5Fpdf?B23373209.

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20

ANTONIO, Cec?lia de Souza. "Ocorr?ncia de bact?rias endof?ticas associadas a variedades de cana-de-a??car cultivadas nos estados: Alagoas e Pernambuco." Universidade Federal Rural do Rio de Janeiro, 2010. https://tede.ufrrj.br/jspui/handle/jspui/1535.

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Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-04-18T20:10:31Z No. of bitstreams: 1 2010 - Cec?lia de Souza Ant?nio.pdf: 1613209 bytes, checksum: e560fdd88ef8707b79c6d4b0120730ec (MD5)<br>Made available in DSpace on 2017-04-18T20:10:31Z (GMT). No. of bitstreams: 1 2010 - Cec?lia de Souza Ant?nio.pdf: 1613209 bytes, checksum: e560fdd88ef8707b79c6d4b0120730ec (MD5) Previous issue date: 2010-04-20<br>CAPES<br>The sugar cane is one of the major agricultural products in Brazil. The crop is able to associate with diazotrophic bacteria (fix nitrogen from the air), that may be located

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21

Kam, Sin-yee. "Application of 16S rRNA gene sequencing in laboratory diagnosis of mycobacteria other than tuberculosis." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31971052.

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22

Sin, Chin-hung. "Identification of streptococci from pigs in Hong Kong using 16S ribosomal RNA gene sequencing /." View the Table of Contents & Abstract, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36434371.

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23

金倩儀 and Sin-yee Kam. "Application of 16S rRNA gene sequencing in laboratory diagnosis of mycobacteria other than tuberculosis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31971052.

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24

Sin, Chin-hung, and 冼展雄. "Identification of streptococci from pigs in Hong Kong using 16S ribosomal RNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B45010900.

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25

Calus, Szymon Tomasz. "Evaluation of nanopore-based sequencing technology for gene marker based analysis of complex microbial communities : method development for accurate 16S rRNA gene amplicon sequencing." Thesis, University of Glasgow, 2018. http://theses.gla.ac.uk/41086/.

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Nucleic acid sequencing can provide a detailed overview of microbial communities in comparison with standard plate-culture methods. Expansion of high-throughput sequencing (HTS) technologies and reduction in analysis costs has allowed for detailed exploration of various habitats with use of amplicon, metagenomics, and metatranscriptomics approaches. However, due to a capital cost of HTS platforms and requirements for batch analysis, genomics-based studies are still not being used as a standard method for the comprehensive examination of environmental or clinical samples for microbial character

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26

CARVALHO, José Roberto Santo de. "Ecologia microbiana de reatores UASB submetidos a diferentes condições de operação para tratar efluente têxtil." Universidade Federal de Pernambuco, 2016. https://repositorio.ufpe.br/handle/123456789/20169.

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Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2017-07-31T14:41:08Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação_Mestrado_Roberto_Carvalho_2016.pdf: 4128480 bytes, checksum: d7d2a90680981c944fb1c8f51356fdf0 (MD5)<br>Made available in DSpace on 2017-07-31T14:41:08Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação_Mestrado_Roberto_Carvalho_2016.pdf: 4128480 bytes, checksum: d7d2a90680981c944fb1c8f51356fdf0 (MD5) Previous issue date: 2016-06-28<br

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27

Carvalho, Elen Bethleen de Souza, and 92-99187-9362. "Estudo da microbiota da rizosfera do guaranazeiro (Paullinia cupana Kunth var.sorbilis (Mart.) Ducke) pelo sequenciamento do gene 16S rDNA." Universidade Federal do Amazonas, 2015. http://tede.ufam.edu.br/handle/tede/5832.

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Submitted by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2017-08-24T13:58:37Z No. of bitstreams: 2 Tese - Elen B. S. Carvalho.pdf: 1542638 bytes, checksum: 747fad508eb1974ab20e86fd0cfa2904 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)<br>Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2017-08-24T13:58:51Z (GMT) No. of bitstreams: 2 Tese - Elen B. S. Carvalho.pdf: 1542638 bytes, checksum: 747fad508eb1974ab20e86fd0cfa2904 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8

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Ng, Ho-yin Ricky, and 吳浩然. "Identification of anaerobic, non-sporulating, Gram-positive bacilli from blood cultures by 16S rRNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B44670424.

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29

Patuzzi, Ilaria. "16S rRNA gene sequencing sparse count matrices: a count data simulator and optimal pre-processing pipelines." Doctoral thesis, Università degli studi di Padova, 2018. http://hdl.handle.net/11577/3426369.

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The study of microbial communities has deeply changed since it was firstly introduced in the 17th century. In the late 1970s, a breakthrough in the way bacterial communities were studied was brought by the discovery that ribosomal RNA (rRNA) genes could be used as molecular markers to perform organisms classification. Some decades later, the advent of DNA sequencing technology revolutionized the study of microbial communities, permitting a culture-independent view on the overall community contained within a sample. Today, one of the most widely used approaches for microbial communities profili

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30

MUNDELL, J. NICOLE. "PHYLOGENETIC ANALYSIS OF KENTUCKY STRAINS OF XYLELLA FASTIDIOSA." UKnowledge, 2005. http://uknowledge.uky.edu/gradschool_theses/406.

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Phytopathogenic bacterium, Xylella fastidiosa, causes a number of economically important diseases, including Pierces disease (PD) of grape and bacterial leaf scorch (BLS) of a number of landscape trees. In Kentucky (KY), BLS affects a number of shade trees including many oak and maple species. In 2001, PD was diagnosed in grapevines in western KY. Xylella fastidiosa is also detected in many asymptomatic landscape plants and grasses. It was the goal of this research to identify hosts of X. fastidiosa around KY and use phylogenetic analysis to compare sequences of the 16S rDNA and gyrase B (gyrB

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31

Scuderi, Maria Cristina. "Identificazione molecolare di lattobacilli umani ed epidemiologia delle resistenze." Thesis, Universita' degli Studi di Catania, 2011. http://hdl.handle.net/10761/306.

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Quarantacinque ceppi di lattobacilli umani sono stati identificati mediante tecniche molecolari (16S rDNA PCR-RFLP; multiplex PCR 16S-ITS-23S rDNA e regione fiancheggiante la 23S rDNA, multiplex PCR sul gene tuf). Le attivita' in vitro verso differenti classi antibiotiche (beta-lattamici, macrolidi, lincosammidi, fluorochinoloni, glicopeptidi e aminoglicosidi) sono state determinate mediante test di microdiluizione in terreno liquido e diffusione in terreno solido. E' stato effettuato lo studio genotipico della resistenza ai chinoloni mediante amplificazione e sequenziamento delle QRDR (Quinol

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32

Santos, Viviane Piccin dos. "Filogenia molecular de cianobactérias baseada em sequências do 16S-23S-ITS rDNA e PC-IGS : investigação de transferência lateral do PC /." Rio Claro : [s.n.], 2011. http://hdl.handle.net/11449/87867.

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Orientador: Maria do Carmo Bittencourt de Oliveira<br>Banca: Luiz Henrique Zanini Branco<br>Banca: Mariana Cabral de Oliveira<br>Resumo: As cianobactérias apresentam uma ampla variabilidade fenotípica e ecológica. Porém, esta variabilidade, muitas vezes, não corresponde à sua diversidade genética. Assim, o uso de marcadores moleculares é fundamental para os estudos de filogenia neste grupo. Entretanto, a filogenia molecular enfrenta um desafio na seleção dos marcadores devido à ocorrência relativamente frequente da transferência de genes de forma lateral entre os procariotos. Em cianobactérias

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33

胡國良 and Kwok-leung Wu. "In silico analysis of 16S ribosomal RNA gene sequencing based methods for identification of medically important Gram-positive rods." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B40721802.

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Wu, Kwok-leung. "In silico analysis of 16S ribosomal RNA gene sequencing based methods for identification of medically important Gram-positive rods." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B40721802.

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35

Jackson, Matthew Albert. "Novel approaches to analysing 16S ribosomal RNA gene sequencing profiles of the gut microbiota in human health research." Thesis, King's College London (University of London), 2018. https://kclpure.kcl.ac.uk/portal/en/theses/novel-approaches-to-analysing-16s-ribosomal-rna-gene-sequencing-profiles-of-the-gut-microbiota-in-human-health-research(87401bf4-e566-429f-94f7-40cf9d8a3fef).html.

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There is an increasing body of research associating the gut microbiome with human health. This has been fuelled by the development of untargeted techniques to profile whole microbial communities. A common approach is to sequence the 16S rRNA gene as a marker for taxonomic quantification. However, the results of such analyses are of limited use. The high-dimensional sequencing data are typically reduced to operational taxonomic units (OTUs), which are purely analytical units with no direct biological interpretation. Furthermore, experimental and analytical variation can lead to poor reproducibi

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Santos, Viviane Piccin dos [UNESP]. "Filogenia molecular de cianobactérias baseada em sequências do 16S-23S-ITS rDNA e PC-IGS: investigação de transferência lateral do PC." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/87867.

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Made available in DSpace on 2014-06-11T19:23:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-17Bitstream added on 2014-06-13T19:28:52Z : No. of bitstreams: 1 santos_vp_me_rcla.pdf: 692384 bytes, checksum: 87577700198a680d7662c5fe0990efe7 (MD5)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>As cianobactérias apresentam uma ampla variabilidade fenotípica e ecológica. Porém, esta variabilidade, muitas vezes, não corresponde à sua diversidade genética. Assim, o uso de marcadores moleculares é fundamental para os estudos de filogenia neste grupo. Entretanto, a fi

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37

Bergström, Jennie. "Nested PCR for distinguishing Haemophilus haemolyticus from Haemophilus influenzae and Cloning and expression of fragmented Moraxella catarrhalis IgD-binding protein in E. coli." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8014.

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<p>ABSTRACT</p><p>Nontypable Haemophilus influenzae is a common cause of otitis, sinusitis and conjunctivitis. It is the most common bacterial pathogen associated with chronic obstructive pulmonary disease (COPD). Studies have shown that nonpathogenic Haemophilus haemolyticus are often mistaken for Haemophilus influenzae due to an absent hemolytic reaction on blood agar. Distinguishing H. haemolyticus from H. influenzae is important to prevent unnecessary antibiotic use, and to understand the role of H. influenzae in clinical infections. In this study, PCR-primers for amplifying 16S rDNA seque

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Danckert, Nathan Peter. "Characterising the intestinal microbiome of Australian aquacultured abalone." Thesis, The University of Sydney, 2019. https://hdl.handle.net/2123/21643.

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Abalone are marine molluscs that are highly prized seafood delicacies. Australia produces more than 1,000 metric tonnes of abalone from aquaculture. Abalone aquaculture research priorities are to improve the growth rates and reduce the mortalities of abalone in aquaculture. Currently, little is known about the microbial communities associated with Australian abalone aquaculture; however, pathogens are associated with high mortality rates in the summer months. By utilising the 16S rRNA gene and high-throughput sequencing, the broad aim of this thesis was to characterise the microbiomes of Austr

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39

Leung, Po-shan. "In silico analysis of 16S ribosomal RNA gene sequencing based methods for identification of medically important Gram-positive cocci /." View the Table of Contents & Abstract, 2007. http://sunzi.lib.hku.hk/hkuto/record/B38348159.

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Leung, Po-shan, and 梁寶珊. "In silico analysis of 16S ribosomal RNA gene sequencing based methods for identification of medically important Gram-positive cocci." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B45011382.

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Yeung, Shiu-yan, and 楊兆恩. "Update and evaluation of 16SpathDB, an automated comprehensive database for identification of medically important bacteria by 16S rRNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/193552.

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Identification of pathogens is one of the important duties of clinical microbiology laboratory. Traditionally, phenotypic tests are used to identify the bacteria. However, due to some limitations of the phenotypic tests, the bacteria may not be identified sometimes and cannot be identified promptly. 16S rRNA gene sequencing is a rapid and accurate method to achieve this target. It is especially useful for identify rare or slow growing bacteria. However, the interpretation of the 16S rRNA gene sequencing result is one of the challenging duties to laboratory technicians and microbiologists. Apar

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Varna, Klaidas. "Pienarūgščių bakterijų paieška ir jų identifikavimas migruojančių didžiųjų ančių (Anas platyrhynchos) žarnyne naudojant dalinių 16S rRNR geno sekų analizę ir kultivavimu paremtus metodus." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2007~D_20090908_194033-88862.

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Pienarūgščių bakterijų paieška ir jų identifikavimas migruojančių didžiųjų ančių (Anas platyrhynchos) žarnyne naudojant dalinių 16S rRNR geno sekų analizę ir kultivavimu paremtus metodus Klaidas VARNA Vilniaus Universiteto Ekologijos Institutas, Hidrobiontų Ekologijos ir Fiziologijos Laboratorija bei Populiacinės Genetikos Laboratorija, Akademijos-2, Vilnius-21, 08412, Lietuva. Šiame tyrime pavasarinių ir rudeninių didžiųjų ančių (Anas platyrhynchos) migrantų iš Nemuno deltos virškinamojo trakto pieno rūgšties bakterijų įvairovė buvo ištirta naudojant molekulinius metodus (polimerazės grandini

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Fisher, Marc Lewis. "Comparison of Subterranean Termite (Rhinotermitidae: Reticulitermes) Gut Bacterial Diversity Within and Between Colonies and to Other Termite Species Using Molecular Techniques (ARDRA and 16S rRNA Gene Sequencing)." Diss., Virginia Tech, 2006. http://hdl.handle.net/10919/27379.

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Termites are known to harbor within their gut a diverse assemblage of symbiotic microorganisms. Little work has been done, however, to describe the diversity and function of the bacteria in the economically important eastern subterranean termite, Reticulitermes flavipes. The first object of this study was to characterize the bacterial diversity in the gut of R. flavipes using amplified rDNA restriction analysis (ARDRA) and 16S rRNA gene sequencing. It was determined that ARDRA was an effective technique for characterizing the diversity of the termite gut microbiota. Of the 512 clones analyz

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Moore, Joe Dallas. "Probing Nano-Specific Interactions Between Bacteria and Antimicrobial Nanoparticles Using Microbial Community Changes and Gene Expression." Research Showcase @ CMU, 2017. http://repository.cmu.edu/dissertations/1069.

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Antimicrobial engineered nanomaterials (ENM) are increasingly incorporated into products despite limited understanding of the interactions between ENMs and bacteria that lead to toxic impacts. The hazard posed by increasing environmental release of antimicrobial ENMs is also poorly characterized. The overall objective of this thesis is to inform questions about the types of interactions that lead to an ENM inducing bacterial toxicity. Many antimicrobial ENMs are soluble, and the ion plays an important role in their toxicity. Some believe that, beyond release of ions, ENM toxicity is expected t

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O'Reilly, Guzene. "Assessment of the physico-chemical and microbiological quality of household water in the Vaalharts irrigation scheme, South Africa / G. O'Reilly." Thesis, North-West University, 2012. http://hdl.handle.net/10394/9190.

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Water quality in the Vaalharts region in the Northern Cape Province, South Africa, decreased over the past few years and there was a need for the microbiological and physico-chemical assessment. This problem was identified through discussions with Vaalharts Water (Vaalharts Water User Association) in 2010 when the issue of the impact of deteriorating water quality on drinking water production was raised. It was thus important to investigate concerns of the water users association pertaining to water quality issues. The aim of this study was to assess the physico-chemical and microbiological qu

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Sjöholm, Billie, and Babak Bahrami. "Prevalence and Identification of Lactobacillus Species Isolated from Infected Root Canals by MALDI-TOF Mass Spectrometry, 16S rRNA Gene Sequencing and API 50 CHL." Thesis, Umeå universitet, Tandläkarutbildning, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-97871.

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Lactobacillus, a part of the commensal oral microflora, is frequently found in infected root canals but is not considered to be an endodontic pathogen. Lactobacilli have proven to be difficult to identify on species level with biochemical and gene sequencing methods. MALDI-TOF is a new identification method and to our knowledge it has not been used on lactobacilli from infected root canals. The aims of this study were to evaluate the prevalence of lactobacilli in infected root canals and to examine if MALDI-TOF is a suitable method for identifying lactobacilli species. In the retrospective stu

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Souza, Roberto Antonio de. "Identificação de linhagens atípicas de Yersinia spp. por métodos moleculares." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/60/60135/tde-26052009-161112/.

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O gênero Yersinia compreende 12 espécies. Y. enterocolitica, Y. pseudotuberculosis e Y. pestis são patógenos de vários animais, incluindo os humanos. Y. aldovae, Y. bercovieri, Y. frederiksenii, Y. intermedia, Y. kristensenii, Y. aleksiciae, Y. mollaretti e Y. rhodei são encontradas sobretudo no meio ambiente e alimentos e consideradas, usualmente, como bactérias oportunistas não-patogênicas e Y. ruckeri é um importante patógeno de peixes. Usualmente, as linhagens de Yersinia são classificadas em espécies de acordo com suas características bioquímicas. O Laboratório Nacional de Referência em Y

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Aviles, Bryan. "Influence of Physiological State, Prolonged Dry Storage, and Passage through Simulated Digestion on the Survival and Gene Expression of Salmonella enterica sv. Tennessee." Thesis, Virginia Tech, 2012. http://hdl.handle.net/10919/77002.

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Salmonella enterica serotypes have been linked to outbreaks associated with low water activity foods. The ability of biofilm forming pathogens, such as Salmonella, to survive thermal and chemical processes is improved; it is unclear if biofilms will also improve survival to desiccation and gastric stresses. The purpose of this study was to quantify the effect of physiological state (planktonic versus biofilm) and prior exposure to desiccation on Salmonella survival and gene expression after passage through an in-vitro digestion model. Cells of Salmonella enterica serotype Tennessee were depos

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Abbasabadi, Mahsa. "Microbial colonisation of low-quality fodders in the bovine rumen." Thesis, Queensland University of Technology, 2022. https://eprints.qut.edu.au/236799/1/221128_PhD%2BThesis_Mahsa%2BAbbasabadi.pdf.

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Sugarcane bagasse has a recalcitrant structure and is considered low-quality fodder. This thesis used thermochemical processes to convert sugarcane bagasse into nutritionally enhanced animal feed. Thermochemical treatments were developed at pilot scale to destruct bagasse structure and improve its digestibility. The digestibility and microbial colonisation of untreated and treated sugarcane bagasse samples were evaluated in the bovine rumen. Alkaline thermochemical treatment improved the digestibility of bagasse and changed the microbial communities that were involved in the degradation of bag

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Yamaguti, Mauricio. "Isolamento de micoplasma de suínos com problemas respiratórios e tipificação dos isolados pela PFGE e seqüenciamento do gene 16S rRNA." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-29102009-091226/.

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As doenças respiratórias são responsáveis, na suinocultura, por grandes perdas econômicas e entre os agentes destacam-se os micoplasmas. Foram coletadas 126 amostras de muco nasal/tonsilar, e fragmentos de 78 pulmões, 2 de traquéia e 2 de tonsila. No isolamento foi utilizado o meio FRIIS modificado, na identificação, a Multiplex-PCR e na tipificação, a PFGE e sequenciamento do gene 16S rRNA. Foram obtidos 59 isolados identificados como M. hyopneumoniae (1,70%), M. flocculare (3,40%) e M. hyorhinis (94,90%). A PFGE dos isolados de M. hyorhinis, resultou em 10 e 9 perfis com a enzima AvaI e XhoI

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