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Journal articles on the topic '16S rdna of pseudomonas fluorescens'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Teshome, Befekadu, Eleni Belay, Barkelegne Mengesha, Zerihun Tsegaye, Edwin K. Akley, and Awuku Frederick. "Selection and identification of Pseudomonas and Bacillus Rhizobacteria with Bioinoculant potential for Sorghum." International Journal of Science, Technology, Engineering & Mathematics 5, no. 2 (2025): 38–59. https://doi.org/10.53378/ijstem.353191.

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This study aimed to select and identify elite strains of plant growth-promoting rhizobacteria (PGPR) among phosphate-solubilizing bacteria (PSB) conserved at the Ethiopian Biodiversity Institute. In-vitro screening for plant growth-promoting traits and abiotic stress tolerance identified 12 promising PGPR isolates. These strains exhibited phosphate solubilization (index 1.8–3.2), auxin production, nitrogen fixation, and ammonia production. All isolates showed drought tolerance, and some exhibited salt tolerance (up to 10% NaCl). 16S rDNA sequencing identified Pseudomonas frederiksbergensis, Ps

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Rangel-Castro, J. Ignacio, Jolanta J. Levenfors, and Eric Danell. "Physiological and genetic characterization of fluorescentPseudomonasassociated withCantharellus cibarius." Canadian Journal of Microbiology 48, no. 8 (2002): 739–48. http://dx.doi.org/10.1139/w02-062.

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Fluorescent Pseudomonas spp. isolated from fruiting bodies (FB) of Cantharellus cibarius were characterized physiologically and genetically and were compared with fluorescent Pseudomonas from forest soil and with sequences from the GenBank database. Pseudomonas spp. from FB differed physiologically from isolates from soil lacking FB and had some similarities with the strains obtained from soil underneath the FB. Analyses of the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) patterns and partial sequencing analysis of the 16S-rDNA region indicated that the b

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Fu, Yan-Mei, Hong-Bin Liu, and Xiao-Qin Wu. "Diversity and Function of Endo-Bacteria in Bursaphelenchus xylophilus from Pinus massoniana Lamb. in Different Regions." Forests 11, no. 5 (2020): 487. http://dx.doi.org/10.3390/f11050487.

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The pine wood nematode (PWN) Bursaphelenchus xylophilus is the pathogen that causes pine wilt disease (PWD), a devastating forest disease. PWN-associated bacteria may play a role in PWD. However, little is known about the endo-bacteria in PWN. We analyzed the diversity of endo-bacteria in nine isolates of PWNs from Pinus massoniana Lamb. in nine epidemic areas from three Chinese provinces by high-throughput sequencing of 16S rDNA and isolated and identified culturable endo-bacteria through construction of a 16S rDNA phylogenetic tree and Biolog microbial identification. We also examined the ef

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Huang, Zhong Hua, Xue Pu, Hui Zhang, Fang Yuan, and Zheng Li Liu. "Biodegradation of 2, 4-Dinitrotoluene by Pseudomonas fluorescens sp. Isolated from a Nitrobenzene Contaminated Soil." Advanced Materials Research 610-613 (December 2012): 1233–36. http://dx.doi.org/10.4028/www.scientific.net/amr.610-613.1233.

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A 2,4-Dinitrotoluene degrading bacterium was isolated from sludge contaminated by DNT and identiﬁed as a member of Pseudomonas fluorescens sp. based on 16S rDNA sequence. Under growth substrates limited conditions, the strain was enriched with 2,4-DNT as its sole source of carbon and energy in the groundwater environment. Results indicate the isolate grew optimally at 12(±4)°C and initial pH 7.5 in the mineral salts medium supplemented 2,4-DNT with 40mg/L, thus showing potential for the remediation of 2,4-DNT contaminated groundwater.

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Agbo, Ejiofor C., Obiora R. Ejinaka, Obiorah E. Okechukwu, Obeta M. Uchejeso, Jwanse I. Rinpan, and Ifeoma E. Lote-Nwaru. "Molecular Identification of Biofilm-forming Pseudomonas aeruginosa Isolates from Noncritical Surfaces of a Tertiary Healthcare Center in Abia State, Southeast Nigeria." Journal of Biochemistry, Microbiology and Biotechnology 10, no. 1 (2022): 25–30. http://dx.doi.org/10.54987/jobimb.v10i1.659.

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The presence of biofilm-forming Pseudomonas aeruginosa on noncritical surfaces in a hospital has been attributed to healthcare-associated infections (HAIs) leading to multidrug-resistant (MDR) infections. This study is aimed at identifying the molecular characteristics of Biofilm forming Pseudomonas aeruginosa found on non-critical surfaces in a hospital environment. Samples were collected using the swab technique from different noncritical surfaces surrounding hospitalized patients in different wards namely sphygmomanometers, thermometers, bed rails etc. Bacterial analysis was performed by us

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Cho, Jae-Chang, and James M. Tiedje. "Biogeography and Degree of Endemicity of Fluorescent Pseudomonas Strains in Soil." Applied and Environmental Microbiology 66, no. 12 (2000): 5448–56. http://dx.doi.org/10.1128/aem.66.12.5448-5456.2000.

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ABSTRACT Fluorescent Pseudomonas strains were isolated from 38 undisturbed pristine soil samples from 10 sites on four continents. A total of 248 isolates were confirmed as Pseudomonas sensu stricto by fluorescent pigment production and group-specific 16S ribosomal DNA (rDNA) primers. These isolates were analyzed by three molecular typing methods with different levels of resolution: 16S rDNA restriction analysis (ARDRA), 16S-23S rDNA intergenic spacer-restriction fragment length polymorphism (ITS-RFLP) analysis, and repetitive extragenic palindromic PCR genomic fingerprinting with a BOX primer

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Lucon, Cleusa Maria Mantovanello, Milena Apetito Akamatsu, and Ricardo Harakava. "Promoção de crescimento e controle de tombamento de plântulas de pepino por rizobactérias." Pesquisa Agropecuária Brasileira 43, no. 6 (2008): 691–97. http://dx.doi.org/10.1590/s0100-204x2008000600004.

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O objetivo deste trabalho foi avaliar o efeito de rizobactérias, no crescimento de plântulas de pepino e no controle de tombamento, causado por Pythium aphanidermatum. Foram realizados em laboratório ensaios de: degradação de 1-aminociclopropano-1-carboxilato (ACC); colonização das raízes de plântulas de pepino; e pareamento de culturas. A identificação dos melhores isolados foi feita pela determinação das seqüências do gene 16S rDNA. Trinta e sete isolados, dos 165 testados, aumentaram a massa de matéria seca das plantas de pepino em até 63%. Desses, somente um isolado (N13 - Pseudomonas fluo

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Khezrinejad, Nabi, Gholam Khodakaramian, and Fatemeh Shahryari. "Characterization of potential plant growth-promoting rhizobacteria isolated from sunflower (Helianthus annuus L.) in Iran." Biologia Futura 70, no. 4 (2019): 268–77. http://dx.doi.org/10.1556/019.70.2019.30.

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Purpose This study aims to characterize plant growth-promoting rhizobacteria (PGPR) in sunflowers growing in different locations at North West of Iran. Materials and methods Sunflower plants were collected from different regions of West Azarbaijan, and rhizospheric bacterial strains were isolated and screened for PGP traits. Identification and characterization of the PGPR were conducted based on 16s rDNA sequences and phenotypic analysis, the strains clustered for genetic diversity by rep-PCR method. Results Among the 80 bacterial isolates, 20 showed PGP traits and were selected for other pote

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Bodilis, Josselin, and Sylvie Barray. "Molecular evolution of the major outer-membrane protein gene (oprF) of Pseudomonas." Microbiology 152, no. 4 (2006): 1075–88. http://dx.doi.org/10.1099/mic.0.28656-0.

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The major outer-membrane protein of Pseudomonas, OprF, is multifunctional. It is a non-specific porin that plays a role in maintenance of cell shape, in growth in a low-osmolarity environment, and in adhesion to various supports or molecules. OprF has been studied extensively for its utility as a vaccine component, its role in antimicrobial drug resistance, and its porin function. The authors have previously shown important differences between the OprF and 16S rDNA phylogenies: Pseudomonas fluorescens isolates split into two quite separate clusters, probably according to their ecological niche

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Guo, Chang Hong, Xu Han Shi, and Jia Li Liu. "Isolation of ACC Deaminase-Containing Plant Growth-Promoting Rhizobacteria from Porrum Rhizasphere Soil." Advanced Materials Research 356-360 (October 2011): 240–43. http://dx.doi.org/10.4028/www.scientific.net/amr.356-360.240.

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A bacterial strain 3-1 which could use ACC as the sole nitrogen sources was isolated from Chinese chives rhizosphere soil. The strain was identified as Pseudomonas fluorescent, according to the results of morphology, physiology and the phylogenetical analyses of 16S rDNA sequence. The ACC deaminase activity of Pseudomonas fluorescent 3-1 strain had high level of ACC deaminase activity, 2.63±0.12 µmol α-KA • (mg Pr •h)-1 . The procedure described here was relatively straight forward to isolate and characterize new PGPR strains. This bacterium may be used to facilitate the growth of Porrum.

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KAHALA, MINNA, LUCIA BLASCO, and VESA JOUTSJOKI. "Molecular Characterization of Spoilage Bacteria as a Means To Observe the Microbiological Quality of Carrot." Journal of Food Protection 75, no. 3 (2012): 523–32. http://dx.doi.org/10.4315/0362-028x.jfp-11-185.

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This study characterized the bacteria causing decay of carrots during storage and marketing. Spoilage strains were identified by 16S–amplified rDNA restriction analysis and intergenic transcribed spacer–PCR–restriction fragment length polymorphism (ITS-PCR-RFLP). Genotypic fingerprinting by RFLP–pulsed-field gel electrophoresis was used to assess the genetic diversity of the isolates. A total of 252 Pseudomonas isolates from carrots were identified and classified into eight separate groups. Most strains belonged to group A (Pseudomonas fluorescens, Pseudomonas marginalis, and Pseudomonas veron

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Abo-Elyousr, Kamal A. M., Maged M. Saad, Adel D. Al-Qurashi, Omer H. M. Ibrahim, and Magdi A. A. Mousa. "Management of Cumin Wilt Caused by Fusarium oxysporum Using Native Endophytic Bacteria." Agronomy 12, no. 10 (2022): 2510. http://dx.doi.org/10.3390/agronomy12102510.

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Eleven endophytic bacteria were isolated from the roots of cumin, a medicinal plant grown in Assiut Province, Egypt. The strains KAU2025 and KAU2022 exhibited the highest inhibition rate against Fusarium oxysporum (FOC7) (64%) in dual culture test and were selected for further studies. KAU2022 and KAU2025 were identified as Brevibacillus parabrevis and Pseudomonas fluorescens, respectively, based on 16S rDNA sequence analysis. The isolates increased the seed germination of cumin by 46.6% compared to that in the control infected with the pathogen F. oxysporum (FOC7). Additionally, the biocontro

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Said, Layla Abdulhamed, Sawsan Saeed Hasan, and Saad L. Hamed. "Identification of Clinical Pseudomonas spp. by VITEK 2 Compact System and Species-specific Polymerase Chain Reaction Assay for Identification of Pseudomonas aeruginosa." International Journal of Drug Delivery Technology 10, no. 03 (2020): 383–88. http://dx.doi.org/10.25258/ijddt.10.3.14.

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The objective of this study was to isolate, identify, and diagnose Pseudomonas spp. from different clinical sources in Baghdad, Iraq. VITEK 2 compact system identification gram-negative bacteria (ID gNB) cards were used to confirm the identification. Polymerase chain reaction (PCR) technique and sequencing were used for recognition of the 16S rDNA gene, by two pairs of primers, universal primers (930 bp fragments) for recognition of Pseudomonas spp., and Pseudomonas aeruginosa specific species (PASS) primers (956 bp fragments) for differentiation of P. aeruginosa from other species. Amplified

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McSpadden Gardener, Brian B., and David M. Weller. "Changes in Populations of Rhizosphere Bacteria Associated with Take-All Disease of Wheat." Applied and Environmental Microbiology 67, no. 10 (2001): 4414–25. http://dx.doi.org/10.1128/aem.67.10.4414-4425.2001.

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ABSTRACT Take-all, caused by Gaeumannomyces graminis var. tritici, is one of the most important fungal diseases of wheat worldwide. Knowing that microbe-based suppression of the disease occurs in monoculture wheat fields following severe outbreaks of take-all, we analyzed the changes in rhizosphere bacterial communities following infection by the take-all pathogen. Several bacterial populations were more abundant on diseased plants than on healthy plants, as indicated by higher counts on a Pseudomonas-selective medium and a higher fluorescence signal in terminal restriction fragment length pol

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Joshi, Bhavesh M., Mrugesh M. Patel, Rudra B. Parmar, N. K. Singh, and P. V. Tapre. "Molecular Characterization of Plant Growth Promoting Pseudomonas from Rhizosphere of Euphorbia caducifolia Haines." International Journal of Bio-resource and Stress Management 13, no. 11 (2022): 1240–51. http://dx.doi.org/10.23910/1.2022.3262.

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A study was conducted during kharif 2020 at the Department of Microbiology, Chimanbhai Patel College of Agriculture, S. D. Agricultural University for molecular characterization of plant growth promoting Pseudomonas isolates from rhizosphere of Euphorbia caducifolia. Twenty Pseudomonas isolates (designated as PsEc1 to PsEc20) were axenically isolated from rhizospheric soil sample of Euphorbia caducifolia using Pseudomonas Isolation Agar Base (a selective medium). These isolates were tested gram negative, rod shaped, and oxidase positive. All 20 isolates were positive for utilization of maltose

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Mawad, Asmaa M. M., Wael S. Abdel-Mageed, and Abd El-Latif Hesham. "Quantification of Naphthalene Dioxygenase (NahAC) and Catechol Dioxygenase (C23O) Catabolic Genes Produced by Phenanthrene-Degrading Pseudomonas fluorescens AH-40." Current Genomics 21, no. 2 (2020): 111–18. http://dx.doi.org/10.2174/1389202921666200224101742.

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Background: Petroleum polycyclic aromatic hydrocarbons (PAHs) are known to be toxic and carcinogenic for humans and their contamination of soils and water is of great environmental concern. Identification of the key microorganisms that play a role in pollutant degradation processes is relevant to the development of optimal in situ bioremediation strategies. Objective: Detection of the ability of Pseudomonas fluorescens AH-40 to consume phenanthrene as a sole carbon source and determining the variation in the concentration of both nahAC and C23O catabolic genes during 15 days of the incubation

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Milijasevic-Marcic, Svetlana, Biljana Todorovic, Milos Stepanovic, et al. "Monitoring of bacterial diseases of Agaricus bisporus in Serbia." Pesticidi i fitomedicina 31, no. 1-2 (2016): 29–35. http://dx.doi.org/10.2298/pif1602029m.

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Monitoring of button mushroom bacterial diseases was conducted to estimate the presence and identity of mycopathogenic bacteria and to determine the predominant bacterial pathogen in Serbia. Samples were collected from mushroom farms during 2006- 2010 and also from fresh markets during 2014-2015. The collected samples showed either symptoms of brown blotch or different degrees of brown discoloration on caps and stalks of Agaricus bisporus resembling bacterial infection. The presence of bacterial droplets on gills was not recorded. The isolated bacteria were Gram-negative and fluorescent on Kin

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Singh, Ruby, O. Colin Stine, David L. Smith, John K. Spitznagel, Mohamed E. Labib, and Henry N. Williams. "Microbial Diversity of Biofilms in Dental Unit Water Systems." Applied and Environmental Microbiology 69, no. 6 (2003): 3412–20. http://dx.doi.org/10.1128/aem.69.6.3412-3420.2003.

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ABSTRACT We investigated the microbial diversity of biofilms found in dental unit water systems (DUWS) by three methods. The first was microscopic examination by scanning electron microscopy (SEM), acridine orange staining, and fluorescent in situ hybridization (FISH). Most bacteria present in the biofilm were viable. FISH detected the β and γ, but not the α, subclasses of Proteobacteria. In the second method, 55 cultivated biofilm isolates were identified with the Biolog system, fatty acid analysis, and 16S ribosomal DNA (rDNA) sequencing. Only 16S identified all 55 isolates, which represente

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Landa, Blanca B., Dmitri M. Mavrodi, Linda S. Thomashow, and David M. Weller. "Interactions Between Strains of 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens in the Rhizosphere of Wheat." Phytopathology® 93, no. 8 (2003): 982–94. http://dx.doi.org/10.1094/phyto.2003.93.8.982.

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Strains of fluorescent Pseudomonas spp. that produce the antibiotic 2,4-diacetylphoroglucinol (2,4-DAPG) are among the most effective rhizobacteria controlling diseases caused by soilborne pathogens. The genotypic diversity that exists among 2,4-DAPG producers can be exploited to improve rhizosphere competence and biocontrol activity. Knowing that D-genotype 2,4-DAPG-producing strains are enriched in some take-all decline soils and that P. fluorescens Q8r1-96, a representative D-genotype strain, as defined by whole-cell repetitive sequence-based polymerase chain reaction (rep-PCR) with the BOX

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Jami Al-Ahmadi, Majid, Abbas Mohammadi, and Esmaeil Salehi Kohabadi. "Characterization of Bacteria Isolated from the Saffron (Crocus sativus L.) Rhizosphere." Journal of Horticultural Research 25, no. 2 (2017): 5–14. http://dx.doi.org/10.1515/johr-2017-0017.

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Abstract One purpose of assessing the soil alive and active community is the identification of beneficial bacteria to use them as biological fertilizers, replacing or supplementing synthetic fertilizers. Such biofertilizers are predicted for the sustainability of agricultural production, especially for low input systems such as saffron fields. The aim of this work was to isolate and identify saffron rhizobacteria and to evaluate their possible effects on saffron growth. During 2013/14, some bacteria were isolated from the rhizosphere of the saffron plantations of different age in Gol village,

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Marques, Eder, Rafaela CF Borges, and Carlos H. Uesugi. "Identification and pathogenicity of Pseudomonas cichorii associated with a bacterial blight of gerbera in the Federal District." Horticultura Brasileira 34, no. 2 (2016): 244–48. http://dx.doi.org/10.1590/s0102-053620160000200015.

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ABSTRACT: In 2013, leaf samples of gerbera plants showing symptoms of bacterial blight were collected in cut-flower high tunnels, in the settlement of núcleo rural alexandre gusmão, located in brazlândia, distrito federal, brazil. seven isolates obtained were subjected to phenotypic and molecular characterization, including pathogenicity tests, lopat, and partial sequencing of the 16s rdna gene. all isolates were gram-negative, aerobic, oxidase-positive, produced fluorescent pigment, induced hypersensitivity in tobacco leaves, used sorbitol and glutamate and were pathogenic to 24 different pla

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Ali, Avin O., and Tavga S. Rashid. "Biocontrol Activities of Olive Endophytic Bacteria Isolates Against Pseudomonas savastanoi." Polytechnic Journal 12, no. 2 (2023): 114–20. http://dx.doi.org/10.25156/ptj.v12n2y2022.pp114-120.

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Background and objectives: Olive knot disease caused by the phytopathogenic bacterium Pseudomonas savastanoi affects olive cultivation in both quality and quantity. Endophytic bacteria are candidates for biocontrol agents according to previous research. Methods: In this study we collected samples (leaves and galls) randomly for pathogen and endophytic bacteria isolation from the different olive growing areas around Erbil including (Qucha Blbas, Grdasor, Sami Abdulrahman Park, and College of Agricultural Engineering Sciences) on June - October 2021. Results: Forty-six isolates of endophytic bac

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Hassanshahian, Mehdi. "Isolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils." Iranian Journal of Toxicology 10, no. 3 (2016): 13–20. http://dx.doi.org/10.32598/ijt.10.4.357.1.

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Background: Organic agricultural pesticides are so stable in ecosystems and biosphere. These compounds affect soil conditions and agricultural products. The aim of this study was isolation of diazinon-degrading bacteria from Kerman pistachio orchards, Southeastern Iran. Methods: Diazinon-degrading bacteria were enriched in Bushnell-Hass medium. Identification and sequencing of prevalent degrading strains was performed using PCR based on amplifying of 16s rDNA. Results: The soil of pistachio orchard has some degrading bacteria that are suitable for elimination of diazinon from soil and environm

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Neykova, I., and S. Shilev. "Compost and Beneficial Pseudomonas Populations Promote Enzyme Activity, Amino Acids and Polymers Utilization Patterns in Heavy Metal Contaminated Soils." Acta Microbiologica Bulgarica 40, no. 1 (2024): 84–96. http://dx.doi.org/10.59393/amb24400111.

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PGPR-assisted phytoremediation is a biotechnology offering opportunities to improve the state of contaminated soil ecosystems. The present study has investigated the potential of six beneficial isolates tolerant to Cd, Pb, and Zn and identified through 16S rDNA sequential analysis as Bacillus cereus, Pseudomonas putida, and Pseudomonas fluorescens to stimulate spinach growth and soil microbial community development in heavy metal-contaminated soil with or without the addition of compost. We found improved plant growth in all treatments with compost, but especially applying consortiums of isola

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Li, Binbin, Shuji Liu, Xiaoting Chen, et al. "Dynamic Changes in the Microbial Composition and Spoilage Characteristics of Refrigerated Large Yellow Croaker (Larimichthys crocea) during Storage." Foods 12, no. 21 (2023): 3994. http://dx.doi.org/10.3390/foods12213994.

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The quality changes, dynamic changes in microbial composition, and diversity changes in large yellow croaker (Larimichthys crocea) during 4 °C refrigeration were studied using 16S rDNA high-throughput sequencing technology, and the total viable count (TVC), total volatile basic nitrogen (TVB-N), and thiobarbituric acid-reactive substances (TBARS) were determined. The results revealed a consistent increase in TVC, TVB-N, and TBARS levels over time. On the 9th day, TVC reached 7.43 lg/(CFU/g), while on the 15th day, TVB-N exceeded the upper limit for acceptable quality, reaching 42.56 mg/100 g.

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Moretti, C., R. Fakhr, and R. Buonaurio. "Calendula officinalis: A New Natural Host of Pseudomonas viridiflava in Italy." Plant Disease 96, no. 2 (2012): 285. http://dx.doi.org/10.1094/pdis-08-11-0691.

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In November 2010, small necrotic spots surrounded by chlorotic halos, which sometimes enlarged and coalesced to form large dead areas, were observed on leaves of marigold (Calendula officinalis L.) plants grown in the Medieval Garden at the Agricultural Faculty of Perugia (central Italy). Cream-colored bacterial colonies were consistently isolated on nutrient agar (NA) from the diseased leaf tissues. Four representative selected strains, which were gram negative, fluorescent on King's medium B, and had oxidative but not fermentative metabolism, were subjected to a pathogenicity test by inocula

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LAGUERRE, G., L. RIGOTTIER-GOIS, and P. LEMANCEAU. "Fluorescent Pseudomonas species categorized by using polymerase chain reaction (PCR)/restriction fragment analysis of 16S rDNA." Molecular Ecology 3, no. 5 (1994): 479–87. http://dx.doi.org/10.1111/j.1365-294x.1994.tb00126.x.

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Yang, Ming-Ming, Dmitri V. Mavrodi, Olga V. Mavrodi, et al. "Biological Control of Take-All by Fluorescent Pseudomonas spp. from Chinese Wheat Fields." Phytopathology® 101, no. 12 (2011): 1481–91. http://dx.doi.org/10.1094/phyto-04-11-0096.

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Take-all disease of wheat caused by the soilborne fungus Gaeumannomyces graminis var. tritici is one of the most important root diseases of wheat worldwide. Bacteria were isolated from winter wheat from irrigated and rainfed fields in Hebei and Jiangsu provinces in China, respectively. Samples from rhizosphere soil, roots, stems, and leaves were plated onto King's medium B agar and 553 isolates were selected. On the basis of in vitro tests, 105 isolates (19% of the total) inhibited G. graminis var. tritici and all were identified as Pseudomonas spp. by amplified ribosomal DNA restriction analy

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Mølbak, Lars, Tine Rask Licht, Thomas Kvist, Niels Kroer, and Sigrid Rita Andersen. "Plasmid Transfer from Pseudomonas putida to the Indigenous Bacteria on Alfalfa Sprouts: Characterization, Direct Quantification, and In Situ Location of Transconjugant Cells." Applied and Environmental Microbiology 69, no. 9 (2003): 5536–42. http://dx.doi.org/10.1128/aem.69.9.5536-5542.2003.

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ABSTRACT The transfer of the plasmids pJKJ5 and TOL (pWWO) from Pseudomonas putida to the indigenous bacterial community on alfalfa sprouts was studied. Tagging with fluorescent protein markers allowed direct quantification of the introduced donor bacteria and of indigenous bacteria that had received the plasmids. The sprouts were observed for 9 days; during this time alfalfa seeds, inoculated with donor bacteria, developed to edible and subsequently decaying sprouts. The first transconjugants were detected on day 6 after donor inoculation and occurred at frequencies of 3.4 × 10−4 and 2.0 × 10

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Gumtow, R. L., A. A. Khan, A. M. Bocsanczy, J. M. F. Yuen, A. J. Palmateer, and D. J. Norman. "First Report of a Leaf Spot Disease of Golden Dewdrop (Duranta erecta) Caused by Pseudomonas cichorii and a Xanthomonas Species in Florida." Plant Disease 97, no. 6 (2013): 836. http://dx.doi.org/10.1094/pdis-12-12-1117-pdn.

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Duranta erecta (Verbenaceae) is used extensively in southern states as an ornamental shrub and has replaced boxwood as the most common short hedge accenting flower beds. Over the past 2 years, during warm wet periods, dark necrotic leaf spots have been observed on golden dewdrop plants in Florida. Isolations from these spots on Difco nutrient agar (NA) consistently yielded two types of bacterial colonies that were not always simultaneously present: 1) round butyrous, bright yellow and 2) flat cream-colored. Both were 2 mm in size after 48 h, gram-negative, and produced a hypersensitivity react

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Koike, Steven T., Jeri D. Barak, Diana M. Henderson, and Robert L. Gilbertson. "Bacterial Blight of Leek: A New Disease in California Caused by Pseudomonas syringae." Plant Disease 83, no. 2 (1999): 165–70. http://dx.doi.org/10.1094/pdis.1999.83.2.165.

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During 1996 and 1997, a new and damaging disease of leek (Allium porrum) was observed on greenhouse-produced transplants and field-grown plants in California. Symptoms were water-soaked lesions at leaf tips, which eventually expanded down the length of the leaf and resulted in brown, elongated, stripe-like lesions with yellow margins. Diseased leaves eventually wilted. A blue fluorescent pseudomonad was consistently recovered from lesions, and biochemical and physiological tests indicated that it was Pseudomonas syringae. Pathogenicity tests established that representative strains of this P. s

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Faltin, Franziska, Jana Lottmann, Rita Grosch, and Gabriele Berg. "Strategy to select and assess antagonistic bacteria for biological control ofRhizoctonia solaniKühn." Canadian Journal of Microbiology 50, no. 10 (2004): 811–20. http://dx.doi.org/10.1139/w04-063.

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A screening strategy was developed to assess the potential of plant-associated bacteria to control diseases caused by Rhizoctonia solani Kühn. About 434 already characterized antagonistic bacterial strains isolated from diverse plant species and microenvironments were evaluated for biocontrol and plant growth promotion by a hierarchical combination of assays. Analyzing in vitro antagonism towards different Rhizoctonia isolates resulted in a selection of 20 potential biocontrol agents. The strains were characterized by their antagonistic mechanisms in vitro as well as their production of the pl

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Cai, Xianghai, Siqi Chen, Hong Yang, et al. "Biodegradation of waste greases and biochemical properties of a novel lipase from Pseudomonas synxantha PS1." Canadian Journal of Microbiology 62, no. 7 (2016): 588–99. http://dx.doi.org/10.1139/cjm-2015-0641.

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A lipase-producing bacterial strain was isolated from oil-well-produced water in Shengli oilfield (Shandong province, China) and was identified as Pseudomonas synxantha by 16S rDNA sequence analysis (named Pseudomonas synxantha PS1). Strain PS1 showed a maximum lipase activity of 10.8 U/mL after culturing for 48 h at 30 °C, with lactose (4 g/L) as carbon source, tryptone (8 g/L) as nitrogen source, olive oil (0.5%, v/v) as inductor, and the initial pH 8.0. Meanwhile, the lipase gene from P. synxantha PS1 was cloned and expressed in Escherichia coli BL21 with the vector pET28a. The novel gene (

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Zhou, L. H., Y. Han, G. H. Ji, Z. S. Wang, and F. Liu. "First Report of Bacterial Leaf Spot Disease Caused by Pseudomonas syringae pv. syringae on Panax notoginseng." Plant Disease 97, no. 5 (2013): 685. http://dx.doi.org/10.1094/pdis-11-12-1047-pdn.

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Panax notoginseng is a species that produces a rare type of Chinese herbal medicine and is cultivated primarily in Yunnan Province. P. notoginseng has a 3-year-long crop cycle before harvest. A new bacterial disease was observed on P. notoginseng plants in the Wenshan Mountain area of Yunnan in 2012. The disease affected primarily leaves. Symptoms were circular or irregular brown leaf spots, surrounded by a yellow halo, located on the edges of the leaves. Eight creamy white pigmented, rounded strains were isolated consistently from leaf spots on Luria-Bertani agar (LB) medium, incubated at 28°

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Godebo, Ashebir T., James J. Germida, and Fran L. Walley. "Isolation, identification, and assessment of soil bacteria as biocontrol agents of pea root rot caused by Aphanomyces euteiches." Canadian Journal of Soil Science 100, no. 3 (2020): 206–16. http://dx.doi.org/10.1139/cjss-2019-0133.

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Aphanomyces euteiches is a soil-borne pathogen that causes root rot of pea and can significantly affect pea production in western Canada. This study aimed to isolate and identify soil bacteria with antagonistic activity towards A. euteiches mycelial and zoospore developmental stages under in vitro conditions and assess their potential as biocontrol agents against aphanomyces root rot in field pea under growth chamber conditions. In vitro screening of soil bacteria identified 184 antagonistic isolates, including 22 from an existing culture collection. Mean mycelial growth inhibition zones range

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Yu, S. M., and Y. H. Lee. "First Report of Pseudomonas cichorii Associated with Leaf Spot on Soybean in South Korea." Plant Disease 96, no. 1 (2012): 142. http://dx.doi.org/10.1094/pdis-08-11-0653.

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Unusual symptoms were observed on 5% of the soybean (Glycine max (L.) Merill) plants in commercial fields in South Korea in September 2008 and 2009. The lesions were at first water soaked, then enlarged and turned dark brown or black, often with concentric white rings and sometimes surrounded by a bright yellow halo. Most lesions were roughly circular to irregular. Six bacteria were isolated on tryptic soy agar (TSA) media from plants of soybean cv. Daewon. The isolate JBC1 formed colonies that were whitish to greenish, circular with convex elevation, and unable to grow anaerobically or at 37°

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Ha, Thi Quyen, and Thi Thu Ha CHU. "Selection of nitrogen fixation and phosphate solubilizing bacteria from cultivating soil samples of Hung Yen province in Vietnam." Journal of Vietnamese Environment 12, no. 2 (2020): 162–68. http://dx.doi.org/10.13141/jve.vol12.no2.pp162-168.

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   The nitrogen fixing bacteria (NFB) and phosphate solubilizing bacteria (PSB) are used widely for producing of microbiological fertilizers. This study aims to seek nitrogen-fixing and phosphate-solubilizing bacteria strains to add to the collection of candidate strains for producing single and multi-function microbiological fertilizers. From 40 soil samples of 8 fields for cultivating rice, medicinal plants and vegetables, 15 NFB strains and 12 PSB strains were isolated and determined the ability of fixing nitrogen and solubilizing inorganic phosphate compound through creat

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Rudi, Knut, Signe L. Flateland, Jon Fredrik Hanssen, Gunnar Bengtsson, and Hilde Nissen. "Development and Evaluation of a 16S Ribosomal DNA Array-Based Approach for Describing Complex Microbial Communities in Ready-To-Eat Vegetable Salads Packed in a Modified Atmosphere." Applied and Environmental Microbiology 68, no. 3 (2002): 1146–56. http://dx.doi.org/10.1128/aem.68.3.1146-1156.2002.

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ABSTRACT There is a clear need for new approaches in the field of microbial community analyses, since the methods used can be severely biased. We have developed a DNA array-based method that targets16S ribosomal DNA (rDNA), enabling the direct detection and quantification of microorganisms from complex communities without cultivation. The approach is based on the construction of specific probes from the 16S rDNA sequence data retrieved directly from the communities. The specificity of the assay is obtained through a combination of DNA array hybridization and enzymatic labeling of the construct

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Khabbaz, Salah Eddin, and Pervaiz A. Abbasi. "Isolation, characterization, and formulation of antagonistic bacteria for the management of seedlings damping-off and root rot disease of cucumber." Canadian Journal of Microbiology 60, no. 1 (2014): 25–33. http://dx.doi.org/10.1139/cjm-2013-0675.

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Antagonistic bacteria are common soil inhabitants with potential to be developed into biofungicides for the management of seedling damping-off, root rot, and other soil-borne diseases of various crops. In this study, antagonistic bacteria were isolated from a commercial potato field and screened for their growth inhibition of fungal and oomycete pathogens in laboratory tests. The biocontrol potential of the 3 most effective antagonistic bacteria from the in vitro tests was evaluated against seedling damping-off and root rot of cucumber caused by Pythium ultimum. Based on phenotypic characteris

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Sharma, Garima, Shweta Dang, Sanjay Gupta, and Reema Gabrani. "IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF BACTERIA HAVING ANTIMICROBIAL AND ANTIBIOFILM ACTIVITY." International Journal of Pharmacy and Pharmaceutical Sciences 8, no. 10 (2016): 111. http://dx.doi.org/10.22159/ijpps.2016v8i10.12338.

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<p><strong>Objective:</strong> The aim of the current study was to isolate and identify the bacteriocinogenic strain exhibiting broad range antimicrobial activity and antibiofilm activity from soil of animal farms.</p><p><strong>Methods:</strong> In the current study, bacterial strains were isolated from soil of twelve different regions of animal farm all over India and screened for antimicrobial activity against<em> Staphylococcus epidermidis</em>, <em>Micrococcus luteus, Pseudomonas fluorescence</em> and <em>Escherichia

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R.A., Neware, Bhagat Y.S., Ambadkar C.V., et al. "Morphological, Biochemical and Molecular Characterization of Plant Growth Promoting Rhizobacteria with Synergistic Effect against Fusarium oxysporum." Journal of Advances in Biology & Biotechnology 27, no. 11 (2024): 295–311. http://dx.doi.org/10.9734/jabb/2024/v27i111613.

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Currently, plant growth-promoting rhizosphere bacteria (PGPR) are heavily exploited as microbial inoculants in agricultural production among which Pseudomonas sp. and Bacillus sp. are widely used in plant growth promotion and disease control as excellent inoculum strains. Plant growth-promoting rhizobacteria has the potential to be used in agriculture to promote plant growth and health through various mechanisms. Therefore, identifying novel strains tailored to specific agricultural requirements continues to be a key area of research. In the present research work, the strains of PGPR isolated

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González, A. J., G. González-Varela, and F. J. Gea. "Brown Blotch Caused by Pseudomonas tolaasii on Cultivated Pleurotus eryngii in Spain." Plant Disease 93, no. 6 (2009): 667. http://dx.doi.org/10.1094/pdis-93-6-0667b.

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In 2006, symptoms of brown blotch were observed on cultivated Pleurotus eryngii (king oyster mushroom) in Castilla-La Mancha (Spain). Subsequently, between January and May of 2008, brown blotch affected 39.75% of the blocks of substrate cultivated, resulting in a considerable loss of production. Symptoms observed were principally characterized by a yellowish brown-to-orangish color, first of the cap and then of the stalk. Some samples also showed a slightly concave cap. From samples collected from four different king oyster mushroom farms, a fluorescent gram-negative bacterium was recovered on

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Negoro, Eiju, Hiromichi Iwasaki, Katunori Tai, Satoshi Ikegaya, Mitsunobu Shimadzu, and Takanori Ueda. "Utility of PCR Amplification and DNA Microarray Hybridization in 16S rDNA for Rapid Diagnosis of Bacteremia Associated with Hematological Malignancies." Blood 114, no. 22 (2009): 1635. http://dx.doi.org/10.1182/blood.v114.22.1635.1635.

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Abstract Abstract 1635 Poster Board I-661 Introduction The rapid diagnosis of bacteremia is important for patient management and the choice of antimicrobial and antifungal therapy, especially in hematological malignancies. We describe a rapid detection and identification system that uses universal PCR primers and DNA microarray hybridization compared to the conventional culture method. This new DNA-microarray method could be performed within only 24 hours. Eighty-four microorganism probes are spotted onto a microarray plate containing most causal clinical pathogens (Patent No.WO2003/106676). W

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Balaž, J., R. Iličić, S. Maširević, D. Jošić, and S. Kojić. "First Report of Pseudomonas syringae pv. syringae Causing Bacterial Leaf Spots of Oil Pumpkin (Cucurbita pepo) in Serbia." Plant Disease 98, no. 5 (2014): 684. http://dx.doi.org/10.1094/pdis-07-13-0714-pdn.

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Oil pumpkin (Cucurbita pepo L.) is commonly used for oil production, mainly in central and eastern Europe (1). In Serbia, it grows only in the north (Vojvodina Province), up to 1,500 ha. In June 2008, typical bacterial spot symptoms (dark green, water-soaked, transparent and greasy spots with yellow margins) were observed for the first time, cultivated at the experimental fields near Backi Petrovac. Since then, bacterial spots were regularly observed on oil pumpkin in the beginning of the growing seasons and during rainy weather, with disease incidence ranging from 5 to 20%. Bacteria isolated

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Lee, Y. A., Y. Y. Shiao, and C. P. Chao. "First Report of Burkholderia cepacia as a Pathogen of Banana Finger-Tip Rot in Taiwan." Plant Disease 87, no. 5 (2003): 601. http://dx.doi.org/10.1094/pdis.2003.87.5.601a.

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Banana finger-tip rot is an endemic minor disease in Taiwan. Diseased fingers are recognized externally by their distorted shape, often being narrow at the tip, smaller, and curved out of line with the other fingers. Parts of the pulp within the finger appear slightly gelatinous and yellow. Frequently, a brown discoloration is evident at the tip of the flower end. Although Pseudomonas sp. was reported to be a causal pathogen, the identity of the species was not determined (1). Isolations from diseased fingers consistently yielded bacterial colonies that were whitish-yellow on Luria-Bertani aga

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46

Lamichhane, J. R., G. M. Balestra, and L. Varvaro. "Severe Outbreak of Bacterial Canker Caused by Clavibacter michiganensis subsp. michiganensis on Tomato in Central Italy." Plant Disease 95, no. 2 (2011): 221. http://dx.doi.org/10.1094/pdis-09-10-0635.

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From May to July 2010, severe outbreaks of bacterial canker of tomato (Solanum lycopersicum L.) were observed in 16 fields in the Province of Viterbo, central Italy. Cultivars affected were Uno Rosso, Peto 1296, UG 812, UG 822, and Podium. Disease incidence ranged from 70 to 100% and was highest for Uno Rosso followed by UG 812, UG 822, Peto 1296, and Podium. Leaf symptoms initially appeared as interveinal, pale green, water-soaked areas that quickly turned yellow-brown to necrotic, resembling sunburn. Infected parts of the plants began to wilt and then die. Light yellow-to-brown streaks or ca

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Xu, L. H., G. L. Xie, B. Li, B. Zhu, F. S. Xu, and J. Qian. "First Report of Pear Blossom Blast Caused by Pseudomonas syringae pv. syringae in China." Plant Disease 92, no. 5 (2008): 832. http://dx.doi.org/10.1094/pdis-92-5-0832c.

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In the spring of 2006, a new bacterial disease was noted in pear orchards near Hangzhou, Zhejiang Province, China. The disease caused severe blossom blast on pears (Pyrus pyrifolia; cv. Cuiguan). Early symptoms of the disease included blackening of the calyx end of developing fruit, blackening of blossom clusters while leaves of affected blossom clusters appeared normal, or death of clusters consisting of both blossoms and leaves. Later, tips of twigs turned dark brown and died. No bacterial ooze was observed. Twelve bacterial isolates were recovered from ten samples of buds and blossoms. Six

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48

Balestra, G. M., A. Mazzaglia, and A. Rossetti. "Outbreak of Bacterial Blossom Blight Caused by Pseudomonas viridiflava on Actinidia chinensis Kiwifruit Plants in Italy." Plant Disease 92, no. 12 (2008): 1707. http://dx.doi.org/10.1094/pdis-92-12-1707a.

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During the springs from 2006 to 2008, a new disease was observed on 4- to 5-year-old Actinidia chinensis (cv. Jin Tao) trees in different commercial kiwifruit-production areas in northern Italy (Lombardy). Initially, disease occurrence was sporadic but later became widespread. Symptoms on flowers appeared as a dark brown rot of anthers, filaments, sepals, and whole buds. Blossoms abscised prematurely from buds. Symptoms appeared as confluent brown spots often present on rolled margins. Bacteria were isolated from symptomatic tissue on nutrient agar medium supplemented with 5% sucrose. The isol

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Martín-Sanz, A., J. L. Palomo, M. Pérez de la Vega, and C. Caminero. "First Report of Bacterial Blight Caused by Pseudomonas viridiflava on Pea in Spain." Plant Disease 94, no. 1 (2010): 128. http://dx.doi.org/10.1094/pdis-94-1-0128a.

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Because production of dry peas (Pisum sativum L.) is increasing in Spain, disease surveys were carried out from 2004 to 2006 in Castilla y Leon, the largest pea-producing region. In May of 2004, a leaf and stem blight caused an estimated 25% loss in yield in pea (cv. Messire) fields in El Cerrato (Palencia). Bacteria were isolated on King's B medium from 10 symptomatic plants from different fields (3). Thirty gram-negative isolates produced fluorescent, yellowish mucoid colonies. All isolates showed oxidative glucose metabolism on Hugh-Leifson medium and were levan and oxidase negative, potato

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Bull, C. T., A. I. Huerta, and S. T. Koike. "First Report of Blossom Blight of Strawberry (Fragaria × ananassa) Caused by Pseudomonas marginalis." Plant Disease 93, no. 12 (2009): 1350. http://dx.doi.org/10.1094/pdis-93-12-1350b.

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In 2003, a new disease was observed on commercial strawberries (Fragaria × ananassa Duch.) grown in multiple fields in Watsonville, CA. Initial symptoms consisted of brown lesions on the undersides of the sepals of strawberry flowers. The lesions coalesced and spread to upper sepal surfaces and anther bases. No leaf symptoms were observed. Fields affected with this disease appeared to have a greater number of deformed fruit, though incidence data were not collected. A gram-negative, blue-green fluorescent pseudomonad was isolated from lesions on King's medium B agar from both sepals and anther

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