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Journal articles on the topic '16S rDNA sequence of S. marcescens'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Rascoe, J., M. Berg, U. Melcher, et al. "Identification, Phylogenetic Analysis, and Biological Characterization of Serratia marcescens Strains Causing Cucurbit Yellow Vine Disease." Phytopathology® 93, no. 10 (2003): 1233–39. http://dx.doi.org/10.1094/phyto.2003.93.10.1233.

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A serious vine decline of cucurbits known as cucurbit yellow vine disease (CYVD) is caused by rod-shaped bacteria that colonize the phloem elements. Sequence analysis of a CYVD-specific polymerase chain reaction (PCR)-amplified 16S rDNA product showed the microbe to be a γ-proteobacterium related to the genus Serratia. To identify and characterize the bacteria, one strain each from watermelon and zucchini and several noncucurbit-derived reference strains were subjected to sequence analysis and biological function assays. Taxonomic and phylogenetic placement was investigated by analysis of the

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Zhang, Q., R. Weyant, A. G. Steigerwalt, et al. "Genotyping of Serratia marcescens Strains Associated with Cucurbit Yellow Vine Disease by Repetitive Elements-Based Polymerase Chain Reaction and DNA-DNA Hybridization." Phytopathology® 93, no. 10 (2003): 1240–46. http://dx.doi.org/10.1094/phyto.2003.93.10.1240.

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The bacterium that causes cucurbit yellow vine disease (CYVD) has been placed in the species Serratia marcescens based on 16S rDNA and groE sequence analysis. However, phenotypic comparison of the organism with S. marcescens strains isolated from a variety of ecological niches showed significant heterogeneity. In this study, we compared the genomic DNA of S. marcescens strains from different niches as well as type strains of other Serratia spp. through repetitive elements-based polymerase chain reaction (rep-PCR) and DNA-DNA hybridization. With the former, CYVD strains showed identical banding

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3

Zhong, Baozhu, Chaojun Lv, Wenlian Li, Chaoxu Li, and Tuo Chen. "Virulence of entomopathogenic bacteria Serratia marcescens against the red palm weevil, Rhynchophorus ferrugineus (Olivier)." PeerJ 11 (December 1, 2023): e16528. http://dx.doi.org/10.7717/peerj.16528.

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Background The red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier), is an important quarantine pest, which has caused serious economic losses in various palm species, such as coconut, oil palm and date palm. Finding effective biocontrol resources is important for the control of this pest and the protection of palm crops. Methods A pathogenic strain HJ-01 was isolated from infected and dead pupa of Tenebrio molitor using tissue separation method. The HJ-01 strain was streak cultured and purified, and its morphological, physiological, biochemical characteristics, and 16S rDNA homology wer

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Wick, R. L., J. Lerner, S. D. Pair, J. Fletcher, F. Mitchell, and B. D. Bruton. "Detection of Cucurbit Yellow Vine Disease in Squash and Pumpkin in Massachusetts." Plant Disease 85, no. 9 (2001): 1031. http://dx.doi.org/10.1094/pdis.2001.85.9.1031c.

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Commercial plantings of summer squash in Charlemont, Franklin County, MA, were decimated in 1999 by 100% incidence of a yellowing disease resembling cucurbit yellow vine disease (CYVD) (1). Both plantings were established in the same field during the third week of May, one with transplants and the second by direct-seeding. Each planting consisted of four 30-m rows each of yellow zucchini (Cucurbita pepo cv. Gold Rush), summer squash (C. pepo cv. Seneca Prolific), and zucchini (C. pepo cv. Condor). Crops were produced organically and pyrethrum was used to control a high infestation of squash bu

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Mayar Hezam, Abbas, and Ahmed Majeed Abd Zaid. "Serratia marcescens isolated from newborn meningitis in the Iraqi city of Diwaniyah: Molecular characterization." Sumer 2 8, CSS 2 (2023): 1–8. http://dx.doi.org/10.21931/rb/css/2023.08.02.94.

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Our study was conducted to detect virulence genes in Serratia marcescens. It has many virulence genes that cause nosocomal infections in immunocompromised persons and neonates. A total of 24/100 (24%) S. marcescens were obtained from neonates suffering from meningitis, and they were identified using culture characteristics biochemical- tests and confirmed by Polymerase chain reaction (PCR) technique, using the 16S rRNA gene. All virulence factors, including the fimA gene that encodes type-1 fimbria, the bsmB gene that encodes exo polysaccharide production, and ampC that encodes ß-lactamase enz

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Deng, Cheng Xun, Mei Yue, Ling Wang, and Yu Hui Li. "Molecular Identification of a Strain Acidithiobacillus Ferrooxidans and its Biological Characteristics." Advanced Materials Research 402 (November 2011): 3–6. http://dx.doi.org/10.4028/www.scientific.net/amr.402.3.

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A strain of Acidithiobacillus ferrooxidans designated S t1 was isolated and identified from the acid mine drainage of Tianmashan Coal Mine, Tongling county, Anhui province. Its morphological, physiological and biochemical characters, growth conditions of pH value and temperature, 16S rDNA sequence and the phylogenetic tree was studied. Results showed that the bacteria strains from different sites have divergence at the curve of pH and Eh values during the enriching culture. The isolated strain S t1 grew in a pH rang from 2.0 to 3.0 and temperature 25 to 35°C with over 98％similarity toAcidithio

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Ma, Xiping, Xianzhu Li, Wanlong Li, Di Wang, Chengbin Xu, and Xuelian Meng. "Identification and characterization of a cold-adapted and halotolerant nitrobenzene-degrading bacterium." RSC Advances 5, no. 98 (2015): 80276–82. http://dx.doi.org/10.1039/c5ra14280f.

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Niño, Marjohn C., Jerome H. Ruiz, and Maria Lima D. Pascual. "Identification and Characterisation of Endophytic Bacteria in Rice Bean (Vigna umbellata)." Journal of Tropical Biodiversity and Biotechnology 10, no. 2 (2025): jtbb14798. https://doi.org/10.22146/jtbb.14798.

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Rice bean (Vigna umbellata) is an underutilised legume with unexplored endophytic microbiome. Identification and characterisation of these endophytes are critical in understanding their roles in plant’s growth, health, and productivity. In this study, twelve morphologically and biochemically distinct bacterial endophytes were isolated from rice bean roots. Sequence analysis of the 16S rDNA of the isolates revealed that members of Proteobacteria, including Stenotrophomonas sp., Shinella sp., Roseomonas sp., Pantoea dispersa, and Serratia marcescens dominated the root tissues of rice bean. The r

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Coombs, Justin T., and Christopher M. M. Franco. "Isolation and Identification of Actinobacteria from Surface-Sterilized Wheat Roots." Applied and Environmental Microbiology 69, no. 9 (2003): 5603–8. http://dx.doi.org/10.1128/aem.69.9.5603-5608.2003.

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ABSTRACT This is the first report of filamentous actinobacteria isolated from surface-sterilized root tissues of healthy wheat plants (Triticum aestivum L.). Wheat roots from a range of sites across South Australia were used as the source material for the isolation of the endophytic actinobacteria. Roots were surface-sterilized by using ethanol and sodium hypochlorite prior to the isolation of the actinobacteria. Forty-nine of these isolates were identified by using 16S ribosomal DNA (rDNA) sequencing and found to belong to a small group of actinobacterial genera including Streptomyces, Microb

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Rome, Sophie, Jean-Claude Cleyet-Marel, Luis A. Materon, Philippe Normand, and Brigitte Brunel. "Rapid identification of Medicago nodulating strains by using two oligonucleotide probes complementary to 16S rDNA sequences." Canadian Journal of Microbiology 43, no. 9 (1997): 854–61. http://dx.doi.org/10.1139/m97-124.

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Symbiotic bacteria associated with the Medicago genus are separated into two closely related species named Sinorhizobium meliloti and Sinorhizobium medicae. To discriminate rapidly between these two bacterial species, two 15-base DNA probes, 16Smfs and 16Smed, were designed from the alignment of 16S rDNA sequences to differentiate S. meliloti from S. medicae. Their specificities were evaluated by dot-blot hybridization experiments on 25 reference strains representing 13 species of Rhizobium and Sinorhizobium, and by comparison with all 16S rDNA sequences available in the GenBank data base. No

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11

Korobov, V. V., E. Yu Zhurenko, N. V. Zharikova, T. R. Iasakov, and Т. V. Markusheva. "Treatment of Phenol-Containing Wastewater by the Bacterial Strain Serratia marcescens MT9." Ecology and Industry of Russia 26, no. 2 (2022): 39–43. http://dx.doi.org/10.18412/1816-0395-2022-2-39-43.

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The features of the phenol and 2,4-dichlorophenol-degrading strain S. marcescens MT9, isolated from the soil of the Ufa (Republic of Bashkortostan) a major producer of organic chemistry products in Russia, are described. Identification of the strain was performed according to the cultural, morphological, physiological, biochemical, morphometric features, as well as the results of the comparative analysis of the 16S rRNA gene sequence. The growth of S. marcescens MT9 in the batch culture with phenol and 2,4-dichlorophenol as sole source of carbon and energy in concentration 100 mg/l was studied

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12

Tajbakhsh, Mercedeh, Babak Noory Nayer, Kamyar Motavaze, et al. "Phylogenetic relationship of Salmonella enterica strains in Tehran, Iran, using 16S rRNA and gyrB gene sequences." Journal of Infection in Developing Countries 5, no. 06 (2011): 465–72. http://dx.doi.org/10.3855/jidc.1504.

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Introduction: We assessed whether 16S rDNA and gyrB gene sequences, alone or combined, were suitable for determining the phylogenetic relationship among Salmonella enterica strains isolated from Tehran, Iran. Patients over five years of age enrolled in an acute diarrheal surveillance project in Tehran province between May 2004 and October 2006 were selected as our study group. Methodology: 16S ribosomal DNA (rDNA) and gyrB genes from 40 Salmonella isolates obtained from patients with acute diarrhea were sequenced and the data was used to generate phylogenetic trees that facilitated isolate com

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13

Doi, Yohei, Keiko Yokoyama, Kunikazu Yamane, et al. "Plasmid-Mediated 16S rRNA Methylase in Serratia marcescens Conferring High-Level Resistance to Aminoglycosides." Antimicrobial Agents and Chemotherapy 48, no. 2 (2004): 491–96. http://dx.doi.org/10.1128/aac.48.2.491-496.2004.

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ABSTRACT Serratia marcescens S-95, which displayed an unusually high degree of resistance to aminoglycosides, including kanamycins and gentamicins, was isolated in 2002 from a patient in Japan. The resistance was mediated by a large plasmid which was nonconjugative but transferable to an Escherichia coli recipient by transformation. The gene responsible for the aminoglycoside resistance was cloned and sequenced. The deduced amino acid sequence of the resistance gene shared 82% identity with RmtA, which was recently identified as 16S rRNA methylase conferring high-level aminoglycoside resistanc

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Wijanarka, Wijanarka, Sri Pujiyanto, and Budi Raharjo. "Identifikasi Molekular Bakteri Glukanolitik Indigenous KE-B6 dari Saluran Pencernaan Bekicot (Achatina fulica)." JURNAL BIOLOGI PAPUA 11, no. 2 (2019): 64–71. http://dx.doi.org/10.31957/jbp.877.

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Glucanolitic bacteria are bacteria that have the ability to break down glucan into glucose monomer units. The ability of the bacteria is caused by the presence of glucanase enzymes. The choice of glucanase derived from bacteria is based on the ability and speed of bacterial growth in terms of producing glucanase enzymes. The presence of bacteria and protozoa in the digestive tract symbiotic with each other to digest cellulose or concomitant materials . Based on the ability of the way of life to digest forage and leaf litter, it is suspected that snails (Achatina fulica) have the ability to pro

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15

Bhadra, Bhaskar, Pradosh Roy, and Ranadhir Chakraborty. "Serratia ureilytica sp. nov., a novel urea-utilizing species." International Journal of Systematic and Evolutionary Microbiology 55, no. 5 (2005): 2155–58. http://dx.doi.org/10.1099/ijs.0.63674-0.

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A Gram-negative, rod-shaped, urea-dissolving and non-spore-forming bacterium, designated strain NiVa 51T, was isolated from water of the River Torsa in Hasimara, Jalpaiguri district, West Bengal, India. On the basis of 16S rRNA gene sequence similarity, strain NiVa 51T was shown to belong to the γ-Proteobacteria and to be related to Serratia marcescens subsp. sakuensis (98·35 %) and S. marcescens subsp. marcescens (98·30 %); however, strain NiVa 51T exhibited only 43·7 % similarity to S. marcescens by DNA–DNA hybridization. The G+C content of the genomic DNA of the isolate was 60 mol%. Both bi

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Texeira, D. C., J. Ayres, E. W. Kitajima, et al. "First Report of a Huanglongbing-Like Disease of Citrus in Sao Paulo State, Brazil and Association of a New Liberibacter Species, “Candidatus Liberibacter americanus”, with the Disease." Plant Disease 89, no. 1 (2005): 107. http://dx.doi.org/10.1094/pd-89-0107a.

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Huanglongbing (HLB) (ex-greening) is one of the most serious diseases of citrus. The causal agent is a noncultured, sieve tube-restricted α-proteobacterium, “Candidatus Liberibacter africanus” in Africa and “Candidatus Liberibacter asiaticus” in Asia (2). The disease has never been reported from the American continent. However, Diaphorina citri, the Asian psyllid vector of HLB, is found in South, Central, and North America (Florida and Texas). Early in 2004, leaf and fruit symptoms resembling those of HLB were observed in several sweet orange orchards near the city of Araraquara, Sao Paulo Sta

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Nogales, Balbina, Edward R. B. Moore, Enrique Llobet-Brossa, Ramon Rossello-Mora, Rudolf Amann, and Kenneth N. Timmis. "Combined Use of 16S Ribosomal DNA and 16S rRNA To Study the Bacterial Community of Polychlorinated Biphenyl-Polluted Soil." Applied and Environmental Microbiology 67, no. 4 (2001): 1874–84. http://dx.doi.org/10.1128/aem.67.4.1874-1884.2001.

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ABSTRACT The bacterial diversity assessed from clone libraries prepared from rRNA (two libraries) and ribosomal DNA (rDNA) (one library) from polychlorinated biphenyl (PCB)-polluted soil has been analyzed. A good correspondence of the community composition found in the two types of library was observed. Nearly 29% of the cloned sequences in the rDNA library were identical to sequences in the rRNA libraries. More than 60% of the total cloned sequence types analyzed were grouped in phylogenetic groups (a clone group with sequence similarity higher than 97% [98% for Burkholderia andPseudomonas-ty

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Gomes, Eliane Aparecida, Sílvia Neto Jardim, Claudia Teixeira Guimarães, Isabel Regina Prazeres de Souza, and Elizabeth de Oliveira. "Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants." Pesquisa Agropecuária Brasileira 39, no. 1 (2004): 61–65. http://dx.doi.org/10.1590/s0100-204x2004000100009.

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The objective of this work was to characterize the genetic variability of phytoplasma and Spiroplasma kunkelii isolated from maize plants showing symptoms of stunt collected from different Brazilian geographic regions. A DNA fragment of 500 base pairs (bp) was amplified from the spiralin gene in S. kunkelii and one fragment of 1,200 bp was generated from 16S rDNA gene in phytoplasma. The partial sequences of the spiralin gene showed similarity of 98% among the isolates of S. kunkelii analyzed. These sequences were compared with the sequence of the spiralin gene from other Spiroplasma species d

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Layton, A. C., P. N. Karanth, C. A. Lajoie, et al. "Quantification of Hyphomicrobium Populations in Activated Sludge from an Industrial Wastewater Treatment System as Determined by 16S rRNA Analysis." Applied and Environmental Microbiology 66, no. 3 (2000): 1167–74. http://dx.doi.org/10.1128/aem.66.3.1167-1174.2000.

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ABSTRACT The bacterial community structure of the activated sludge from a 25 million-gal-per-day industrial wastewater treatment plant was investigated using rRNA analysis. 16S ribosomal DNA (rDNA) libraries were created from three sludge samples taken on different dates. Partial rRNA gene sequences were obtained for 46 rDNA clones, and nearly complete 16S rRNA sequences were obtained for 18 clones. Seventeen of these clones were members of the beta subdivision, and their sequences showed high homology to sequences of known bacterial species as well as published 16S rDNA sequences from other a

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Valiunas, D., R. Jomantiene, A. Ivanauskas, et al. "First Report of a New Phytoplasma Subgroup, 16SrIII-T, Associated with Decline Disease Affecting Sweet and Sour Cherry Trees in Lithuania." Plant Disease 93, no. 5 (2009): 550. http://dx.doi.org/10.1094/pdis-93-5-0550b.

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During July 2007, sweet (Prunus avium) and sour cherry (P. cerasus) trees exhibiting disease symptoms suggestive of possible phytoplasma infection were observed in a large orchard in the Kaunas Region of Lithuania. Samples of leaf tissue were collected from 13 sweet cherry trees that were affected by a decline disease (designated cherry decline, ChD) characterized by symptoms that included leaf reddening and premature leaf drop and two sour cherry trees exhibiting proliferation of branches and nonseasonal flowering. To assess the diseased trees for phytoplasma infection, DNA was extracted with

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Zhang, Chun Hong, Dong Dong Xiao, Pei Shi Sun, Ping Zou, and Qiu Ling Chen. "Screening, Identification and Characterization of a Cyanoethylated Wastepaper-Degrading Bacteria." Applied Mechanics and Materials 316-317 (April 2013): 349–53. http://dx.doi.org/10.4028/www.scientific.net/amm.316-317.349.

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A cyanoethylated wastepaper-degrading bacterial strain S11 was screened from the surface humus soil in the forest of Yunnan University. The bacterium was identified through analysis of morphological, biological and biochemical features and 16S rRNA sequence. S11 enjoyed above 99% homology with Ochrobactrum sp.CRRI29’s 16S rDNA sequence, therefore the baterium belonged to Ochrobactrum sp. The research on degradation features of S11 revealed that S11 bacterium could effectively degrade cyanoethylated wastepaper under 35°C, pH 11.0, 25% inoculum size and 50g/L substrate concentration; under the o

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Stunžėnas, Virmantas, Romualda Petkevičiūtė, and Gražina Stanevičiūtė. "Phylogeny of Sphaerium solidum (Bivalvia) based on karyotype and sequences of 16S and ITS1 rDNA." Open Life Sciences 6, no. 1 (2011): 105–17. http://dx.doi.org/10.2478/s11535-010-0101-6.

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AbstractThe present work represents the first karyological and molecular characterisation of Sphaerium solidum, a rare European clam. Specimens of S. solidum were collected in Lithuania and Hungary. The modal diploid chromosome number found in both populations was 2n=30. Small, biarmed B chromosomes were found in 42.3% of cells studied in clams from Lithuania and in 11.8% of cells in clams from Hungary. Comparative analysis revealed no significant (P<0.05) interspecific differences in chromosome morphology of S. solidum and that of previously studied S. corneum. DNA sequence analyses of S.

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Munyaneza, J. E., V. G. Sengoda, J. M. Crosslin, J. A. Garzón-Tiznado, and O. G. Cardenas-Valenzuela. "First Report of “Candidatus Liberibacter solanacearum” in Tomato Plants in México." Plant Disease 93, no. 10 (2009): 1076. http://dx.doi.org/10.1094/pdis-93-10-1076a.

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Tomato (Solanum lycopersicum) plants exhibiting symptoms resembling those of permanent yellowing disease (known in Mexico as “permanente del tomate”) that is commonly associated with phytoplasmas (1) were observed in tomato fields in Sinaloa, México in March 2009. Plant symptoms also resembled those caused by “Candidatus Liberibacter solanacearum” infection (2). Affected plants showed an overall chlorosis, severe stunting, leaf cupping, purple discoloration of veins, excessive branching of axillary shoots, and leaf scorching (1,2). Symptom incidence ranged from 18 to 40%. To investigate whethe

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Sandanayaka, W. R. M., V. A. Davis, S. D. G. Marshall, and J. G. Charles. "Detecting and controlling Serratia marcescens in a laboratory colony of Mastrus ridens." New Zealand Plant Protection 68 (January 8, 2015): 445. http://dx.doi.org/10.30843/nzpp.2015.68.5853.

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Mastrus ridens (Hymenoptera Ichneumonidae) is an ectoparasitoid of cocooning Cydia pomonella (codling moth CM) larvae and has been introduced into New Zealand in a classical biocontrol programme Large numbers of M ridens are currently being reared in the laboratory on diapausing CM larvae for release into applegrowing regions Within the rearing colony a bacterial epizootic was discovered that killed both CM larvae and developing M ridens DNA from infected CM larvae was amplified using bacterial 16s rDNA primers with sequencing identifying the bacterium as Serratia marcescens a common species a

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Komaki, Hisayuki, and Tomohiko Tamura. "Differences at Species Level and in Repertoires of Secondary Metabolite Biosynthetic Gene Clusters among Streptomyces coelicolor A3(2) and Type Strains of S. coelicolor and Its Taxonomic Neighbors." Applied Microbiology 1, no. 3 (2021): 573–85. http://dx.doi.org/10.3390/applmicrobiol1030037.

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Streptomyces coelicolor A3(2) is used worldwide for genetic studies, and its complete genome sequence was published in 2002. However, as the whole genome of the type strain of S. coelicolor has not been analyzed, the relationship between S. coelicolor A3(2) and the type strain is not yet well known. To clarify differences in their biosynthetic potential, as well as their taxonomic positions, we sequenced whole genomes of S. coelicolor NBRC 12854T and type strains of its closely related species—such as Streptomyces daghestanicus, Streptomyces hydrogenans, and Streptomyces violascens—via PacBio.

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Li, Z. N., H. Min, Y. Yan, Z. Zhao, W. J. Wu, and Y. F. Wu. "First Report of Syringa oblata and S. reticulata Leafroll Disease in China." Plant Disease 93, no. 3 (2009): 322. http://dx.doi.org/10.1094/pdis-93-3-0322c.

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Syringa oblata is an important ornamental tree widely grown in China. In September of 2008, S. oblata plants exhibiting symptoms of leafroll and yellowing were found in a garden on the Northwest A&F University campus. Samples were collected from this site. Total DNA was extracted from 0.5 g of phloem tissue from leaf midribs and stems of each sample. DNA samples were analyzed with a nested PCR assay using phytoplasma 16S rDNA universal primers R16mF2/R16mR1 followed by specific primers R16F2n/R16R2 (1), which amplified a 1,452- and 1,246-bp product, respectively. We tested all 30 lilac sam

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Siddiqui, Huma, Karin Lagesen, Alexander J. Nederbragt, Lars M. Eri, Stig L. Jeansson, and Kjetill S. Jakobsen. "Pathogens in Urine from a Female Patient with Overactive Bladder Syndrome Detected by Culture-independent High Throughput Sequencing: A Case Report." Open Microbiology Journal 8, no. 1 (2014): 148–53. http://dx.doi.org/10.2174/1874285801408010148.

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Introduction:Overactive bladder syndrome (OAB) is described as urgency, with or without urgency incontinence. A range of medical conditions shares the symptoms of OAB, however the diagnosis is contingent on the exclusion of urinary tract infection (UTI). Knowing that urine dipstick and routine culture of bacteria can miss UTI diagnosis caused by low-count bacteriuria or “difficult-to-culture” pathogens, we examined a case of OAB with a culture-independent approach.Case presentation:A 61-year-old Norwegian female with a long history of urinary symptoms and a diagnosis of OAB was selected as a s

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Rachman, Cinta, Petia Kabadjova, Rosica Valcheva, Hervé Prévost, and Xavier Dousset. "Identification of Carnobacterium Species by Restriction Fragment Length Polymorphism of the 16S-23S rRNA Gene Intergenic Spacer Region and Species-Specific PCR." Applied and Environmental Microbiology 70, no. 8 (2004): 4468–77. http://dx.doi.org/10.1128/aem.70.8.4468-4477.2004.

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ABSTRACT The genus Carnobacterium is currently divided into the following eight species: Carnobacterium piscicola, C. divergens, C. gallinarum, C. mobile, C. funditum, C. alterfunditum, C. inhibens, and C. viridans. An identification tool for the rapid differentiation of these eight Carnobacterium species was developed, based on the 16S-23S ribosomal DNA (rDNA) intergenic spacer region (ISR). PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of this 16S-23S rDNA ISR was performed in order to obtain restriction profiles for all of the species. Three PCR amplicons, which were desi

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Pellegrin, Véronique, Stefan Juretschko, Michael Wagner, and Gilles Cottenceau. "Morphological and Biochemical Properties of aSphaerotilus sp. Isolated From Paper Mill Slimes." Applied and Environmental Microbiology 65, no. 1 (1999): 156–62. http://dx.doi.org/10.1128/aem.65.1.156-162.1999.

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ABSTRACT Four strains of filamentous bacteria were isolated from slimes collected in different paper mill factories. Morphological and physiological characterization of the isolates indicated an affiliation with the genus Sphaerotilus. However, while the physiological properties of the isolates were almost identical, pronounced physiological differences between the isolates andSphaerotilus natans DSM 6575T, DSM 565, and DSM 566 with respect to their ability to metabolize complex polysaccharides, sugars, polyalcohols, or organic acids as carbon sources were detected. In contrast to the analyzed

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Wu, Yeying, Yalan Feng, Mingyang Xue, et al. "Isolation and Identification of Staphylococcus saprophyticus from Diseased Hybrid Sturgeon." Fishes 8, no. 5 (2023): 250. http://dx.doi.org/10.3390/fishes8050250.

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Hybrid sturgeon is an important economic fish species in China. In 2021, a bacterium was isolated from the liver and kidneys of freshwater-farmed hybrid sturgeon in Yichang City, Hubei Province, causing a disease with high mortality and surface bleeding. Through morphological observation, 16S rDNA gene sequence analysis, pathogenicity, an antimicrobial sensitivity test, as well as serum physiological and biochemical analysis, it was identified as Staphylococcus saprophyticus and named E702. The 16S rDNA gene sequence of E702 is highly homologous to S. saprophyticus in GenBank. Phylogenetic ana

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TAWONG, WITTAYA, PONGSANAT PONGCHAROEN, TOMOHIRO NISHIMURA, and WEERACHAI SAIJUNTHA. "Siamcapillus rubidus gen. et sp. nov. (Oculatellaceae), a novel filamentous cyanobacterium from Thailand based on molecular and morphological analyses." Phytotaxa 558, no. 1 (2022): 33–52. http://dx.doi.org/10.11646/phytotaxa.558.1.2.

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Cyanobacterial diversity has been extensively studied; however, scant knowledge exists concerning genetic data and higher accuracy for tropical cyanobacterial diversity. In particular, the existence of unnamed monophyletic genera within the Oculatellaceae requires further investigation. Here, two novel strains (NUACC13 and NUACC14) were isolated from a cyanobacterial mat covering border soil of a small freshwater pond in Phitsanulok Province, Thailand. A polyphasic approach combining genetic (16S rDNA and rpoC1 gene phylogenies, evolutionary distance and ITS secondary structures) and morpholog

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Ahmad, Fazuriana, Mohd Izuan Effendi Halmi, Baskaran Gunasekaran, Wan Lutfi Wan Johari, Mohd Yunus Shukor, and Mohd Arif Syed. "Inhibitive Bacterial MTT Assay for River Monitoring of Heavy Metals." Bioremediation Science and Technology Research 1, no. 1 (2013): 1–7. http://dx.doi.org/10.54987/bstr.v1i1.7.

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 An inhibitive assay for metals using a MTT-bacterium assay system is presented. The assay is based on the ability of respiring bacteria to reduce the tetrazolium dye MTT. Out of the 13 metals screened, silver, mercury and copper exhibit strong inhibition to bacterial respiratory activity. The calculated IC50s for silver, mercury and copper were 0.068, 0.092 and 0.517 mg/L, respectively. The IC50 values obtained for these heavy metals are comparable and in certain cases lower than existing bioassay methods such as Daphnia magna, rainbow trout, Microtoxâ„¢ and papain assays. Works on real

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Foissac, Xavier, Jean-Luc Danet, Leyla Zreik, et al. "Cloning of the spoT Gene of “CandidatusPhlomobacter fragariae” and Development of a PCR-Restriction Fragment Length Polymorphism Assay for Detection of the Bacterium in Insects." Applied and Environmental Microbiology 66, no. 8 (2000): 3474–80. http://dx.doi.org/10.1128/aem.66.8.3474-3480.2000.

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ABSTRACT Marginal chlorosis is a new disease of strawberry in which the uncultured phloem-restricted proteobacterium “CandidatusPhlomobacter fragariae” is involved. In order to identify the insect(s) vector(s) of this bacterium, homopteran insects have been captured. Because a PCR test based on the 16S rRNA gene (rDNA) applied to these insects was unable to discriminate between “P. fragariae” and other insect-associated proteobacteria, isolation of “P. fragariae” genes other than 16S rDNA was undertaken. Using comparative randomly amplified polymorphic DNAs, an amplicon was specifically amplif

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Stefan, Andrei, Jannick Van Cauwenberghe, Craita Maria Rosu, et al. "Nodules of Medicago spp. Host a Diverse Community of Rhizobial Species in Natural Ecosystems." Agronomy 14, no. 9 (2024): 2156. http://dx.doi.org/10.3390/agronomy14092156.

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Biological nitrogen fixation by rhizobia-nodulated legumes reduces the dependence on synthetic nitrogen fertilizers. Identification of locally adapted rhizobia may uncover economically valuable strains for sustainable agriculture. This study investigated the diversity and symbiotic potential of rhizobia associated with three Medicago species from Eastern Romania’s ecosystems. Phenotypic screening ensured that only rhizobial species were retained for molecular characterization. 16S rDNA sequencing clustered the isolates into four distinct groups: Sinorhizobium meliloti, Sinorhizobium medicae, R

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Wanner, Leslie A. "A New Strain of Streptomyces Causing Common Scab in Potato." Plant Disease 91, no. 4 (2007): 352–59. http://dx.doi.org/10.1094/pdis-91-4-0352.

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Common scab is a serious disease of potatoes (Solanum tuberosum) and other root and tuber crops, affecting the quality and market value of these crops. The disease is caused by gram-positive soil bacteria in the genus Streptomyces. A new common scab-causing streptomycete was isolated from scabby potatoes originating in southeastern Idaho. Research has supported a model of horizontal transfer of pathogenicity determinants among streptomycetes, and the new strain has hallmarks of the recently characterized Streptomyces pathogenicity island (PAI); it has genes encoding the synthetase for the path

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Gumtow, R. L., A. A. Khan, A. M. Bocsanczy, J. M. F. Yuen, A. J. Palmateer, and D. J. Norman. "First Report of a Leaf Spot Disease of Golden Dewdrop (Duranta erecta) Caused by Pseudomonas cichorii and a Xanthomonas Species in Florida." Plant Disease 97, no. 6 (2013): 836. http://dx.doi.org/10.1094/pdis-12-12-1117-pdn.

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Duranta erecta (Verbenaceae) is used extensively in southern states as an ornamental shrub and has replaced boxwood as the most common short hedge accenting flower beds. Over the past 2 years, during warm wet periods, dark necrotic leaf spots have been observed on golden dewdrop plants in Florida. Isolations from these spots on Difco nutrient agar (NA) consistently yielded two types of bacterial colonies that were not always simultaneously present: 1) round butyrous, bright yellow and 2) flat cream-colored. Both were 2 mm in size after 48 h, gram-negative, and produced a hypersensitivity react

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Zhang, Yinjun, Jialin Chen, Changsheng Chen, and Shijin Wu. "Isolation of a Bacillus Aryabhattai Strain for the Resolution of (R, S)-Ethyl Indoline-2-Carboxylate to Produce (S)-Indoline-2-Carboxylic Acid." Catalysts 9, no. 2 (2019): 206. http://dx.doi.org/10.3390/catal9020206.

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The strain screened from sludge can selectively hydrolyze (S)-ethyl indoline-2-carboxylate to produce (S)-indoline-2-carboxylic acid. It was identified as the Bacillus aryabhattai strain based on its morphology, metabolism, and 16S rDNA sequence analysis. Glucose and yeast powder were used as the best carbon and nitrogen sources to cultured cells with an initial pH of seven. Subsequently, we optimized the key parameters for selective hydrolysis. Finally, when the substrate concentration had reached 3%, with a 35 °C water bath, a pH of seven, and a speed of 600 rpm, the e.e.p value attained 96%

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Guo, F. L., H. Y. Zhang, X. M. Yu, W. Q. Zhao, D. Q. Liu, and C. Goyer. "First Report of Streptomyces galilaeus Associated with Common Scab in China." Plant Disease 98, no. 5 (2014): 683. http://dx.doi.org/10.1094/pdis-07-13-0699-pdn.

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During a survey of potato scab pathogens in China from 2003 to 2012, a new pathogen was found in Shanxi and Neimenggu provinces. The incidence was approximately 20% of all recovered strains. The lesions caused by the pathogen were slightly raised and similar to those caused by Streptomyces scabies (3). Lesions were excised (approximately 10 mm3) from 40 infected tubers, surface-disinfested with 0.3% NaOCl for 30 s, rinsed in sterile water three times, cut into 5 mm3, then sliced into 1-mm pieces, and plated on water agar amended with ampicillin (50 μg/ml). Plates were incubated at 28°C in the

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Munyaneza, J. E., V. G. Sengoda, J. M. Crosslin, J. A. Garzón-Tiznado, and O. G. Cardenas-Valenzuela. "First Report of “Candidatus Liberibacter solanacearum” in Pepper Plants in México." Plant Disease 93, no. 10 (2009): 1076. http://dx.doi.org/10.1094/pdis-93-10-1076b.

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Bell pepper (Capsicum annuum) plants exhibiting symptoms that resembled those of potato psyllid (Bactericera cockerelli Sulc) damage and “Candidatus Liberibacter solanacearum” infection (2) were observed in a pepper field in La Cruz de Elota, Sinaloa, México in March 2009, with an infection rate of 1.5%. Plants exhibited chlorotic or pale green apical growth and leaf cupping, sharp tapering of the leaf apex, shortened internodes, and an overall stunting (2). Total DNA was extracted from the top whole leaf tissue of nine symptomatic and five asymptomatic pepper plants with cetyltrimethylammoniu

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Mien, Pham Thi, Nguyen Kim Hanh, Vo Hai Thi, Nguyen Minh Hieu, and Dao Viet Ha. "Bacteria associated with soft coral from Mot island - Nha Trang bay and their antimicrobial activities." Tạp chí Khoa học và Công nghệ biển 20, no. 2 (2020): 209–19. http://dx.doi.org/10.15625/1859-3097/20/2/15089.

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Microbial communities associated with invertebrates had been considered as a new source of bioactive compounds. The soft coral associated bacteria in Mot island, Nha Trang bay were isolated, extracted and assessed for antagonistic activity against human and coral pathogens, the strongly active strains were identified by 16S rRNA analysis. The soft coral associated bacterium SCN10 had abcd antibacterial pattern which was named for inhibition towards Bacillus subtilis (pattern a), Escherichia coli (pattern b), Salmonella typhimurium (pattern c) and Serratia marcescens (pattern d). It was the nea

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Abdellatif, Aya A. M., Abdel-Rahman Tahany M.A, Sayed M.A, Ibrahim Dina S.S., and Elmaghraby M.M.K. "ACTIVITY OF SERRATIA SPP. AND BACILLUS SPP. AS BIOCONTROL AGENTS AGAINST MELOIDOGYNE INCOGNITA INFECTING TOMATO." Pakistan Journal of Biotechnology 18, no. (2-3) (2021): 37–47. http://dx.doi.org/10.34016/pjbt.2021.18.2/3.37.

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Background: Tomato )Solanum lycopersicum( is one of the most popular vegetable crops in Egypt. It is affected by a wide range of pests; prominent among them is root-knot nematode (RKN) (Meloidogyne spp.) which is considered as one of the major limiting factors affecting tomato productions worldwide, especially in Egypt. Aim of study: The present study was performed to evaluate the quantitative and qualitative chitionolytic potentials of Serratia, Bacillus megaterium and B. subtilis as well as their nematicidal effect against Meloidogyne incognita under both in vitro and in vivo conditions. Met

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Abdul-Latif, Rafal Moayad, and Amel Hussaein Mussa. "Bioactivity of Rhizospheric Acinetobacter baumannii Siderophore Combined with Antibiotics Against Lower Respiratory Tract Pathogenic Bacteria." Nature Environment and Pollution Technology 24, S1 (2025): 285–91. https://doi.org/10.46488/nept.2024.v24is1.021.

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The study focused on extracting and purifying siderophore produced by Acinetobacter baumannii isolated from rhizospheric soil in Baghdad city and evaluating its bioactivity both independently and in combination with selected antibiotics. Bacterial identification was performed using CHROM agar, biochemical, and physiological tests, with confirmation via PCR amplification of the 16S rDNA housekeeping gene. The siderophore was extracted using ethyl acetate after culturing the bacteria in succinate broth and was purified through HPLC, detected at a wavelength of 403 nm. A total of 38 bacterial iso

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Palero, Ferran, Guillermo Guerao, Paul F. Clark, and Pere Abelló. "Scyllarus arctus (Crustacea: Decapoda: Scyllaridae) final stage phyllosoma identified by DNA analysis, with morphological description." Journal of the Marine Biological Association of the United Kingdom 91, no. 2 (2010): 485–92. http://dx.doi.org/10.1017/s0025315410000287.

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Advanced stages of Scyllarus phyllosoma larvae were collected by demersal trawling during fishery research surveys in the western Mediterranean Sea in 2003–2005. Nucleotide sequence analysis of the mitochondrial 16S rDNA gene allowed the final-stage phyllosoma of Scyllarus arctus to be identified among these larvae. Its morphology is described and illustrated. This constitutes the second complete description of a Scyllaridae phyllosoma with its specific identity being validated by molecular techniques (the first was S. pygmaeus). These results also solved a long lasting taxonomic anomaly of se

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Ferreira, T., C. A. van Reenen, P. Tailliez, S. Pagès, A. P. Malan, and L. M. T. Dicks. "First report of the symbiotic bacterium Xenorhabdus indica associated with the entomopathogenic nematode Steinernema yirgalemense." Journal of Helminthology 90, no. 1 (2014): 108–12. http://dx.doi.org/10.1017/s0022149x14000583.

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AbstractThe entomopathogenic nematode Steinernema yirgalemense is considered a promising agent in the biocontrol of insects. However, little is known about the bacteria living in symbiosis with the nematode. In this study, we have identified the only available bacterial strain (157-C) isolated from S. yirgalemense, as a member of the species Xenorhabdus indica. Identification was based on 16S rDNA, recA, dnaN, gltX, gyrB and infB gene sequence analyses. The relatedness of strain 157-C to the type strain of X. indica (DSM 17 382) was confirmed with DNA–DNA hybridization. The phenotypic characte

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Sun, Yu Ying, Shu Jun Wang, and Ji Quan Zhang. "Cloning and Recombinant Expression of Chitosanase Gene from Bacillus Sp. S-1." Advanced Materials Research 343-344 (September 2011): 994–99. http://dx.doi.org/10.4028/www.scientific.net/amr.343-344.994.

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In this work, we report the characterization of a chitosanase-producing bacterium isolated from soil. This strain was grouped under the genus Bacillus by virtue of its morphological, physiological properties and 16S rDNA gene sequence and named it Bacillus sp. S-1. According to the information of chitosanase full-length sequences deposited in NCBI, a pair of degenerated primes was designed and a partial sequence of chitosanase gene was obtained by polymerase chain reaction (PCR) using Bacillus sp. S-1 genome DNA as the template. A genome walking library was constructed followed as the protocol

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Nedashkovskaya, Olga I., Makoto Suzuki, Marc Vancanneyt, et al. "Salegentibacter holothuriorum sp. nov., isolated from the edible holothurian Apostichopus japonicus." International Journal of Systematic and Evolutionary Microbiology 54, no. 4 (2004): 1107–10. http://dx.doi.org/10.1099/ijs.0.02987-0.

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Strain KMM 3524T was isolated from the holothurian Apostichopus japonicus living in the Sea of Japan. The bacterial strain was pigmented, non-motile, Gram-negative, strictly aerobic and oxidase-, catalase- and β-galactosidase-positive. From the results of 16S rDNA sequence analysis, strain KMM 3524T was found to be related closely to Salegentibacter salegens (98·1 %). DNA–DNA homology between strains KMM 3524T and S. salegens DSM 5424T was 38 %; this showed clearly that the holothurian isolate KMM 3524T belongs to a novel species of the genus Salegentibacter for which the name Salegentibacter

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Ohike, Tatsuya, Tetsuya Matsukawa, Masahiro Okanami, Shin’ichiro Kajiyama, and Takashi Ano. "Biological Control Potential of Streptomyces sp. AR10 Producing Albocycline Isolated from Soil around Ant Nest." Journal of Agricultural Science 10, no. 3 (2018): 54. http://dx.doi.org/10.5539/jas.v10n3p54.

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Fifty actinomycetes were isolated from fifteen soil samples and were screened for their antagonism against fungal plant pathogens by dual culture assay, and one of the strain named AR10 was shown to be most effective in suppression of growth of plant pathogen. An antifungal compound of AR10 was extracted, and purified by TLC and HPLC. As a result of NMR and LC-MS analysis, the antifungal compound was identified as albocycline. AR10 suppressed Rhizoctonia damping-off of cucumber in infection control assay. The 16S rDNA sequence of AR10 shows high sequence similarity to those of genus Streptomyc

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Lee, Ing-Ming, Kristi D. Bottner, Joseph E. Munyaneza, et al. "Carrot Purple Leaf: A New Spiroplasmal Disease Associated with Carrots in Washington State." Plant Disease 90, no. 8 (2006): 989–93. http://dx.doi.org/10.1094/pd-90-0989.

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During the growing seasons of 2003 and 2004, a disease occurred in several carrot crops in south central Washington with symptoms suggestive of infection by phytopathogenic mollicutes (phytoplasmas and spiroplasmas). In the fall, many affected carrot plants exhibited extensive purple or yellow-purple leaf discoloration, general stunting of shoots and taproots, and formation of bunchy, fibrous secondary roots. For detection of the putative causal agents, polymerase chain reaction (PCR) assays were performed using primers specific to phytoplasmas as well as primers specific to plant-pathogenic s

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Urbanaviciene, L., R. Jomantiene, and R. E. Davis. "First Report of Barley as Host of a Phytoplasma Belonging to Group 16SrI, Subgroup B, and Ribosomal Protein Subgroup rpI-B in Lithuania." Plant Disease 89, no. 3 (2005): 339. http://dx.doi.org/10.1094/pd-89-0339a.

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Numerous diseased plants of barley (Hordeum vulgaris L.) exhibiting twisted, abnormally thin and yellowed awns, reduced spikelets, and general stunting and yellowing were observed in fields in the Vilnius and Kaisiadorys regions of Lithuania. The possible association of a phyto-plasma with the disease, termed barley deformation (BaDef), was assessed using polymerase chain reaction (PCR). Three phytoplasma universal primer pairs (P1/P7, R16F2n/R16R2, and rpF1/rpR1) (1,2,4) were employed to amplify ribosomal (r) RNA gene (rDNA) and ribosomal protein (rp) gene sequences. Template DNA extractions

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Acinas, Silvia G., Josefa Antón, and Francisco Rodríguez-Valera. "Diversity of Free-Living and Attached Bacteria in Offshore Western Mediterranean Waters as Depicted by Analysis of Genes Encoding 16S rRNA." Applied and Environmental Microbiology 65, no. 2 (1999): 514–22. http://dx.doi.org/10.1128/aem.65.2.514-522.1999.

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ABSTRACT In a previous study (S. G. Acinas, F. Rodrı́guez-Valera, and C. Pedrós-Alió, FEMS Microbiol. Ecol. 24:27–40, 1997), community fingerprinting by 16S rDNA restriction analysis applied to Mediterranean offshore waters showed that the free-living pelagic bacterial community was very different from the bacterial cells aggregated or attached to particles of more than about 8 μm. Here we have studied both assemblages at three depths (5, 50, and 400 m) by cloning and sequencing the 16S rDNA obtained from the same samples, and we have also studied the samples by scanning electron microscopy

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