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Relevant bibliographies by topics / 16S rDNA sequencing of E. aerogenes

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Academic literature on the topic '16S rDNA sequencing of E. aerogenes'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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Journal articles on the topic "16S rDNA sequencing of E. aerogenes"

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Saha, Mihir Lal, Mist Dilara Akter, Tahsin Khan, Aneesa Ansari, and Mohammad Nurul Islam. "Bacterial load and multi‐drug resistance patterns of some ready‐to‐eat street foods of Dhaka city." Dhaka University Journal of Biological Sciences 27, no. 1 (2018): 27–36. http://dx.doi.org/10.3329/dujbs.v27i1.46408.

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Bacterial load and drug resistance pattern associated with some ready-to-eat (RTE) street foods such as Chatpoti, Fuchka, Singara, Panipuri, Ghugni-muri, Chola and water of Dhaka South City Corporation were investigated. Most of the samples were found to be contaminated and the bacterial load ranged from 2.4 × 104 - 9.2 × 106, 1.2 × 103 - 7.3 × 105 and 1.1 × 103 - 1.6 × 106 cfu/g of aerobic heterotrophic, coliform bacteria and Staphylococcus, respectively. The highest coliform load (7.3 × 105 cfu/ml) was found in the water of Gulistan. The highest aerobic heterotrophic bacteria (9.2 × 106 cfu/

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HWANG, CHIU-CHU, PEI-HUI TSENG, YI-CHEN LEE, et al. "Determination of Histamine in Japanese Spanish Mackerel (Scomberomorus niphonius) Meat Implicated in a Foodborne Poisoning." Journal of Food Protection 82, no. 10 (2019): 1643–49. http://dx.doi.org/10.4315/0362-028x.jfp-19-111.

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ABSTRACT An incident of foodborne poisoning causing illness in seven victims due to ingestion of fried Japanese Spanish mackerel (JS mackerel; Scomberomorus niphonius) meat occurred in September 2014 in Hualien County, eastern Taiwan. Of the two suspected fish meats, one raw sample contained 3,318 ppm of histamine and one fried sample contained 1,906 ppm of histamine, levels which are greater than the potential hazard action level (500 ppm) in most illness cases. Given the allergy-like symptoms of the victims and the high histamine content in the suspected fish samples, this foodborne poisonin

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Aldeewan, Ali, Nawres Jaber, Mohanad Abdulhameed, and Basil Abbas. "Microbial risk assessment of dairy products from retail marketplaces in Basrah province, Iraq." Open Veterinary Journal 14, no. 3 (2024): 779. http://dx.doi.org/10.5455/ovj.2024.v14.i3.4.

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Background: Milk-borne bacteria cause degradation of milk products and constitute a significant risk to public health. Aim: The objectives of present study are to determine the microbiological quality of dairy products and to investigate pathogenic microorganisms. Methods: A total of 60 samples of raw milk, homemade cheese and yogurt were randomly selected from different retail marketplaces in Basrah. The bacteriological and biochemical tests were utilised to identify the pathogens in dairy samples, as well as the molecular technique was used as accurate diagnostic test. Results: The prevalenc

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Hashavya, S., I. Gross, A. Michael-Gayego, N. Simanovsky, and R. Lamdan. "The efficacy of 16S ribosomal DNA sequencing in the diagnosis of bacteria from blood, bone and synovial fluid samples of children with musculoskeletal infections." Journal of Children's Orthopaedics 12, no. 2 (2018): 204–8. http://dx.doi.org/10.1302/1863-2548.12.170049.

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Background Musculoskeletal infections are among the most common bacterial infections in children leading to hospitalization, invasive procedures and prolonged antibiotic administration. Blood, synovial and sometimes tissue cultures are essential for the diagnosis and treatment of musculoskeletal infections; 16S ribosomal DNA (rDNA) sequencing is a novel diagnostic tool for the detection of bacteria. While the yield of 16S rDNA sequencing in synovial fluid was previously assessed, data regarding the efficacy of this method from blood samples or partially treated children with suspected musculos

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Reischl, U., K. Feldmann, L. Naumann, et al. "16S rRNA Sequence Diversity in Mycobacterium celatum Strains Caused by Presence of Two Different Copies of 16S rRNA Gene." Journal of Clinical Microbiology 36, no. 6 (1998): 1761–64. http://dx.doi.org/10.1128/jcm.36.6.1761-1764.1998.

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Direct sequencing of the 16S rRNA gene (16S rDNA) ofMycobacterium celatum isolates showed ambiguities, suggesting heterogeneity. Cloned 16S rDNA yielded two copies of the gene, which differed by insertion of a thymine at position 214 and by additional mismatches. Restriction fragment length polymorphism analysis confirmed the presence of two copies of 16S rDNA within the bacterial chromosome.

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Drancourt, Michel, Claude Bollet, Antoine Carlioz, Rolland Martelin, Jean-Pierre Gayral, and Didier Raoult. "16S Ribosomal DNA Sequence Analysis of a Large Collection of Environmental and Clinical Unidentifiable Bacterial Isolates." Journal of Clinical Microbiology 38, no. 10 (2000): 3623–30. http://dx.doi.org/10.1128/jcm.38.10.3623-3630.2000.

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Some bacteria are difficult to identify with phenotypic identification schemes commonly used outside reference laboratories. 16S ribosomal DNA (rDNA)-based identification of bacteria potentially offers a useful alternative when phenotypic characterization methods fail. However, as yet, the usefulness of 16S rDNA sequence analysis in the identification of conventionally unidentifiable isolates has not been evaluated with a large collection of isolates. In this study, we evaluated the utility of 16S rDNA sequencing as a means to identify a collection of 177 such isolates obtained from environmen

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Liao, Feng, Yilan Xia, Wenpeng Gu, Xiaoqing Fu, and Bing Yuan. "Comparative analysis of shotgun metagenomics and 16S rDNA sequencing of gut microbiota in migratory seagulls." PeerJ 11 (November 3, 2023): e16394. http://dx.doi.org/10.7717/peerj.16394.

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Background Shotgun metagenomic and 16S rDNA sequencing are commonly used methods to identify the taxonomic composition of microbial communities. Previously, we analysed the gut microbiota and intestinal pathogenic bacteria configuration of migratory seagulls by using 16S rDNA sequencing and culture methods. Methods To continue in-depth research on the gut microbiome and reveal the applicability of the two methods, we compared the metagenome and 16S rDNA amplicon results to further demonstrate the features of this animal. Results The number of bacterial species detected by metagenomics graduall

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Chatellier, Sonia, Nathalie Mugnier, Françoise Allard, et al. "Comparison of two approaches for the classification of 16S rRNA gene sequences." Journal of Medical Microbiology 63, no. 10 (2014): 1311–15. http://dx.doi.org/10.1099/jmm.0.074377-0.

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The use of 16S rRNA gene sequences for microbial identification in clinical microbiology is accepted widely, and requires databases and algorithms. We compared a new research database containing curated 16S rRNA gene sequences in combination with the lca (lowest common ancestor) algorithm (RDB-LCA) to a commercially available 16S rDNA Centroid approach. We used 1025 bacterial isolates characterized by biochemistry, matrix-assisted laser desorption/ionization time-of-flight MS and 16S rDNA sequencing. Nearly 80 % of isolates were identified unambiguously at the species level by both classificat

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Kim, Daeyoo, Gil Young Park, Dong Yeon Lee, Dong-Oh Lee, and Youngsik Yoon. "Nanopore 16S Amplicon Sequencing Enhances the Understanding of Pathogens in Medically Intractable Diabetic Foot Infections." Foot & Ankle Orthopaedics 7, no. 4 (2022): 2473011421S0072. http://dx.doi.org/10.1177/2473011421s00723.

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Category: Diabetes Introduction/Purpose: Diabetic foot infections (DFIs) cause substantial morbidity and mortality. The mainstay of the treatment is empiric antibiotics and surgical debridement in severe cases. Methods: In this study, we performed nanopore 16S rDNA sequencing from the debridement specimens of DFIs. Fifty-four surgical debridement specimens obtained from 45 patients with medically intractable DFI were included. The 16S rDNA PCR was performed on each specimen, and Nanopore sequencing was performed for up to 3 h. The reads were aligned to the BLAST database, and the results were

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Wang, Haiyin, Pengcheng Du, Juan Li, et al. "Comparative analysis of microbiome between accurately identified 16S rDNA and quantified bacteria in simulated samples." Journal of Medical Microbiology 63, no. 3 (2014): 433–40. http://dx.doi.org/10.1099/jmm.0.060616-0.

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Although 16S rRNA gene (rDNA) sequencing is the gold standard for categorizing bacteria or characterizing microbial communities its clinical utility is limited by bias in metagenomic studies, in either the experiments or the data analyses. To evaluate the efficiency of current metagenomic methods, we sequenced seven simulated samples of ten bacterial species mixed at different concentrations. The V3 region of 16S rDNA was targeted and used to determine the distribution of bacterial species. The number of target sequences in individual simulated samples was in the range 1–1000 to provide a bett

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Dissertations / Theses on the topic "16S rDNA sequencing of E. aerogenes"

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Ziegler, Katie. "Phylogenetic Analysis of a Group of Enteric Bacteria Based on 16S rDNA Gene Sequencing." Miami University Honors Theses / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1111684418.

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Perim, Júlia Elídia de Lima. "Diversidade microbiana envolvida na ciclagem do nitrogênio em solos de cultivo de cana-de-açúcar no estado de São Paulo." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-28112016-175102/.

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O Brasil é o maior produtor mundial de cana-de-açúcar, sendo o Estado de São Paulo responsável por mais de 50% do total desta produção. Esta cultura tem um enorme impacto sobre a agricultura brasileira e devido a isto, uma melhor compreensão da composição da comunidade microbiana relacionada a ciclagem do nitrogênio (N) nestes solos é de grande importância para o aprimoramento no cultivo da cana-de-açúcar. No entanto, pouco se sabe sobre a relação entre grupos microbianos e essa cultura. Este trabalho visou determinar variações nas comunidades microbianas que participam das transformações do N

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ANTONIO, Cec?lia de Souza. "Ocorr?ncia de bact?rias endof?ticas associadas a variedades de cana-de-a??car cultivadas nos estados: Alagoas e Pernambuco." Universidade Federal Rural do Rio de Janeiro, 2010. https://tede.ufrrj.br/jspui/handle/jspui/1535.

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Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-04-18T20:10:31Z No. of bitstreams: 1 2010 - Cec?lia de Souza Ant?nio.pdf: 1613209 bytes, checksum: e560fdd88ef8707b79c6d4b0120730ec (MD5)<br>Made available in DSpace on 2017-04-18T20:10:31Z (GMT). No. of bitstreams: 1 2010 - Cec?lia de Souza Ant?nio.pdf: 1613209 bytes, checksum: e560fdd88ef8707b79c6d4b0120730ec (MD5) Previous issue date: 2010-04-20<br>CAPES<br>The sugar cane is one of the major agricultural products in Brazil. The crop is able to associate with diazotrophic bacteria (fix nitrogen from the air), that may be located

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CARVALHO, José Roberto Santo de. "Ecologia microbiana de reatores UASB submetidos a diferentes condições de operação para tratar efluente têxtil." Universidade Federal de Pernambuco, 2016. https://repositorio.ufpe.br/handle/123456789/20169.

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Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2017-07-31T14:41:08Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação_Mestrado_Roberto_Carvalho_2016.pdf: 4128480 bytes, checksum: d7d2a90680981c944fb1c8f51356fdf0 (MD5)<br>Made available in DSpace on 2017-07-31T14:41:08Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação_Mestrado_Roberto_Carvalho_2016.pdf: 4128480 bytes, checksum: d7d2a90680981c944fb1c8f51356fdf0 (MD5) Previous issue date: 2016-06-28<br

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Fogler, Kendall Wilson. "Effect of Soil Amendments from Antibiotic Treated Cows on Antibiotic Resistant Bacteria and Genes Recovered from the Surfaces of Lettuce and Radishes: Field Study." Thesis, Virginia Tech, 2018. http://hdl.handle.net/10919/92587.

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Cattle are commonly treated with antibiotics that may survive digestion and promote antibiotic resistance when manure or composted manure is used as a soil amendment for crop production. This study was conducted to determine the effects of antibiotic administration and soil amendment practices on microbial diversity and antibiotic resistance of bacteria recovered from the surfaces of lettuce and radishes grown using recommended application rates. Vegetables were planted in field plots amended with raw manure from antibiotic-treated dairy cows, composted-manure from cows with different historie

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Torres, Adalgisa Ribeiro. "Diversidade genética de enterobactérias endofíticas de diferentes hospedeiros e colonização de Catharantus roseus por endófitos expressando o gene gfp." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-03062005-152149/.

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Bactérias endofíticas são aquelas que habitam o interior de tecidos vegetais, sem causar dano aparente aos mesmos, além de desempenhar funções importantes no processo de adaptação das plantas. Especial interesse tem sido dado a tais bactérias devido ao seu potencial no controle biológico. Por isso, é muito importante estudar a diversidade genética de endófitos, além de avaliar o impacto da introdução de endófitos geneticamente modificados no ambiente. Estudos vêm sendo feitos nesse sentido, mas não com bactérias endofíticas da família Enterobacteriaceae. Desta forma, o presente trabalho tev

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Bonilla, German Andres Estrada. "Efeito da inoculação de bactérias mobilizadoras de fósforo na compostagem e no desenvolvimento da cana-de-açúcar." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/11/11140/tde-29092015-164939/.

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A indústria sucroenérgetica gera grande quantidade de resíduos, sendo a torta de filtro e as cinzas os principais resíduos sólidos. Uma das tecnologias desenvolvidas para o manejo destes resíduos é a compostagem. A aplicação do composto tem mostrado efeitos positivos na cultura da cana-de-açúcar no que diz respeito à fertilização fosfatada. Os objetivos do presente trabalho foram: I. Observar o comportamento das comunidades bacterianas durante a compostagem e o efeito da inoculação de estirpes bacterianas mobilizadoras de fósforo sobre a disponibilidade de fósforo no composto final; II. Avalia

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Kendall, Joshua Robert Allen. "Soil Microbial Ecology Associated with Disease Control of Fusarium oxysporum f. sp.Cucumerinum in Cucumis sativus Cultivation." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1438262404.

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Kanasi, Eleni. "Molecular analysis of the oral microbiota of dental diseases." Doctoral thesis, Umeå : Oral Microbiology, Department of Odontology, Umeå University, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1911.

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Kock, Martha Magdalena. "Characterization and identification of some indigenous Rhizobia using 16S rDNA sequence analysis." Diss., 1999. http://hdl.handle.net/2263/30155.

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ENGLISH : The use of different characteristics (the polyphasic approach) to describe bacterial taxa is a prerequisite for a stable classification. The taxonomy of root- and stem-nodulating rhizobia is in a state of transition. As more legumes are studied, new species and genera of rhizobia are described. It is important to study the indigenous South African rhizobia, as without them a complete rhizobial taxonomy is not possible. Furthermore, strains with superior nitrogen fixation abilities may be discovered. Indigenous strains better adapted to the harsh South African environment are possible

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Book chapters on the topic "16S rDNA sequencing of E. aerogenes"

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Oskay, Mustafa. "Optimization of Colony Polymerase Chain Reaction for the Identification of Actinobacteria by Sequencing of the 16S rDNA." In Protocols of Actinomycetes. CRC Press, 2024. http://dx.doi.org/10.1201/9781003398400-36.

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Urumbil, Sithara K. "METAGENOMICS THROUGH NEXT GENERATION SEQUENCING- A LEAP IN THE EXPLORATION OF THE HIDDEN WORLD OF ENDOPHYTIC MICROBIAL COMMUNITY." In Recent Trends in Endophyte Research. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/nbennurch265.

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Endophytes are microbes residing in plant tissue and they are clandestine masters of plant growth regulation. Quantifying taxon frequency and community membership of this microflora will allow us to assess their potential at unprecedented depth. The generation of highly accurate genomic data with advanced computational tools points to application-based perspectives of these phytomicrobes. Whole metagenome analysis and metagenome analysis of 16S rDNA sequences are indispensable technologies to explore this valuable resource of microorganisms. Applications of metagenomic analysis in the field of

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Wen, Feifei, Kui Huang, Junjie Xu, and Wenqi Huang. "Bacterial 16s rDNA diversity in the gut of Eisenia fetida revealed by metagenomics and high-throughput sequencing technology." In Earthworm Technology in Organic Waste Management. Elsevier, 2024. http://dx.doi.org/10.1016/b978-0-443-16050-9.00001-3.

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Azwai, S. M., E. A. Alfallani, S. K. Abolghait, et al. "Isolation and Molecular Identification of Vibrio spp. by Sequencing of 16S rDNA from Seafood, Meat and Meat Products in Libya: A Descriptive Study." In Innovations in Microbiology and Biotechnology Vol. 4. Book Publisher International (a part of SCIENCEDOMAIN International), 2022. http://dx.doi.org/10.9734/bpi/imb/v4/1949a.

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Poole Sophie, Singhrao Sim K., Chukkapalli Sasanka, et al. "Active Invasion of Porphyromonas gingivalis and Infection-Induced Complement Activation in ApoE." In Advances in Alzheimer’s Disease. IOS Press, 2017. https://doi.org/10.3233/978-1-61499-706-1-119.

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Periodontal disease is a polymicrobial inflammatory disease that leads to chronic systemic inflammation and direct infiltration of bacteria/bacterial components, which may contribute to the development of Alzheimer's disease. ApoE&minus;/&minus;mice were orally infected (n=12) with Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, and Fusobacterium nucleatum as mono-and polymicrobial infections. ApoE&minus;/&minus;mice were sacrificed following 12 and 24 weeks of chronic infection. Bacterial genomic DNA was isolated from all brain tissues except for the F. nu

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"indicate the kinds of microorganisms present in the sample (Maidak et 1997). The technique therefore provides a list of 16S rRNA sequences as surrogate identification of individuals from the sample. The advantage of 16S rDNA sequencing is that it gives an unambiguous indication of the organisms in the community. The disadvantage of this protocol is that it is costly." In Recent Advances in Marine Biotechnology, Vol. 8. CRC Press, 2003. http://dx.doi.org/10.1201/9781482279986-15.

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Conference papers on the topic "16S rDNA sequencing of E. aerogenes"

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Le Borgne, S., J. Jan, J. M. Romero, and M. Amaya. "Impact of Molecular Biology Techniques on the Detection and Characterization of Microorganisms and Biofilms Involved in MIC." In CORROSION 2002. NACE International, 2002. https://doi.org/10.5006/c2002-02461.

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Abstract Recently, the application of molecular techniques has greatly improved our knowledge of microbial diversity and distribution in environmental samples. In this paper, we have reviewed the molecular biology techniques that can be used to study microorganisms and biofilms involved in the MIC phenomenon. These techniques include 16S ribosomal DNA (16S rDNA) gene based techniques. In particular, 16S rDNA sequencing, the construction of 16S rDNAs libraries, Fluorescence In Situ Hybridization (FISH), Denaturing Gradient Gel Electrophoresis (DGGE) and Random Amplification of Polymorphic DNA (

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De Paula, Renato, Cruz St Peter, Ian Alex Richardson, et al. "DNA Sequencing of Oilfield Samples: Impact of Protocol Choices on the Microbiological Conclusions." In CORROSION 2018. NACE International, 2018. https://doi.org/10.5006/c2018-11662.

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Abstract In the last decade, molecular microbiology techniques have significantly expanded the understanding of the resident microflora in hydrocarbon reservoirs and production systems. These methods have been steadily accepted by the industry and are widely viewed as accurate, comprehensive and highly valuable tools that augment or may eventually replace conventional methods. The resulting information has helped operators and service companies to develop better monitoring programs, assess risks and tailor mitigation strategies to control undesired microbial activities in wells, flowlines and

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Le Borgne, S., J. Jan, J. L. Garcia Villalobos, M. Amaya, and J. M. Romero. "Initial Developments of a Database of Bacteria Involved in Microbiologically Influenced Corrosion." In CORROSION 2004. NACE International, 2004. https://doi.org/10.5006/c2004-04058.

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Abstract An initial database of bacteria involved in MIC is proposed. It is composed of bacteria detected by Molecular Biology techniques through the sequencing of their 16S rDNA genes in bio films, consortia and samples collected in sites potentially affected by MIC problems. The bacteria were not isolated and their participation in MIC should be evaluated. The use of such data is discussed and future directions are also proposed to continue this work.

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Bartling, Craig, Angela Minard-Smith, Kate H. Kucharzyk, Jennifer Busch-Harris, and Larry Mullins. "Interpreting Omic Data for Microbially Influenced Corrosion: Lessons from a Case Study Involving a Seawater Injection System." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09445.

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Abstract Microbiologically influenced corrosion (MIC) is a significant source of pitting corrosion affecting oil and gas pipelines, wells, and a variety of surface facilities. Understanding of MIC is greatly enhanced through DNA and protein sequencing technologies. This paper highlights the need to understand the methods used to generate the data, the data quality, and the limitations associated with data interpretation through a case study involving the metagenomics and proteomic analysis of pig envelope debris and seawater samples from various locations within a seawater injection system sus

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Bakhash, Rama Bakhash, Farzana Sulaiman, Mashael Al-Shafai, and Annalisa Terranegra. "The Microbiome and Epigenome Profile in Pediatric Type 1 Diabetes in Qatar." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0202.

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This study focused on Qatar’s pediatric population that has witnessed a steep increase in the incidence of the disease. In order to understand this, we analyzed the blood and stool samples of a pilot group of 21 T1D subjects (age 6-12 years old) for the microbiome composition, Short Chain Fatty Acid (SCFA) levels and methylation profiles using 16s rDNA sequencing, gas Chromatography and Infinium methylation assay respectively.

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