Relevant bibliographies by topics / 16S Rdna Sequencing of P. Aeruginosa

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Academic literature on the topic '16S Rdna Sequencing of P. Aeruginosa'

Author: Grafiati
Published: 3 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "16S Rdna Sequencing of P. Aeruginosa"

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Iliyasu, M. Y., R. A. Bamanga, A. F. Umar, E. B. Agbo, and A. Uba. "16S rDNA Sequencing analysis in identification of some multidrug resistant (MDR) bacterial isolates from clinical specimens." Nigerian Journal of Biotechnology 36, no. 2 (2020): 158–66. http://dx.doi.org/10.4314/njb.v36i2.16.

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Accurate identification of bacterial pathogens from clinical specimens and Multidrug Resistant (MDR) characterization is a key to empirical therapy. Twelve (12) bacteria isolates from blood, urine and faecal samples were selected based on the ability to grow on Luria Bertani (LB) agar medium containing 100μg/ml ampicillin, identified by 16S rDNA PCR and sequencing. Identified isolates tagged; U01, U02, U03, U04, S08, U10 and U11 were from urine specimens, S05, S06, S07 and S12 from stool, while B09 was from blood. The isolates were screened for MDR pattern according to Kirby-Bauer disc diffusi
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Said, Layla Abdulhamed, Sawsan Saeed Hasan, and Saad L. Hamed. "Identification of Clinical Pseudomonas spp. by VITEK 2 Compact System and Species-specific Polymerase Chain Reaction Assay for Identification of Pseudomonas aeruginosa." International Journal of Drug Delivery Technology 10, no. 03 (2020): 383–88. http://dx.doi.org/10.25258/ijddt.10.3.14.

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The objective of this study was to isolate, identify, and diagnose Pseudomonas spp. from different clinical sources in Baghdad, Iraq. VITEK 2 compact system identification gram-negative bacteria (ID gNB) cards were used to confirm the identification. Polymerase chain reaction (PCR) technique and sequencing were used for recognition of the 16S rDNA gene, by two pairs of primers, universal primers (930 bp fragments) for recognition of Pseudomonas spp., and Pseudomonas aeruginosa specific species (PASS) primers (956 bp fragments) for differentiation of P. aeruginosa from other species. Amplified
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P, Beulah Rose Rani,, and Sheeba Rajakumari, DV. "Prevalence and Characterization of Disease-Causing Bacteria Isolated from Pigeons (Columba livia) in India." UTTAR PRADESH JOURNAL OF ZOOLOGY 45, no. 18 (2024): 699–704. http://dx.doi.org/10.56557/upjoz/2024/v45i184487.

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Pigeons harbour and spread numerous zoonotic diseases, including bacterial diseases caused by pathogenic bacterial communities. Pigeons are the hosts for various bacteria, including Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and Streptococcus pyogenes. In the present study, pigeon (Columba livia) from farms and houses were subjected to determine the major causative bacterial pathogens. The samples were collected from the oral cavity, cloaca and ocular regions of pigeons. The distribution of bacteria was tested using the plate count agar method, and
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Arefin, Md Shamsul, Meftahul Jannat Mitu, Shomaia Yasmin Mitu, et al. "Mutational alterations in the QRDR regions associated with fluoroquinolone resistance in Pseudomonas aeruginosa of clinical origin from Savar, Dhaka." PLOS ONE 20, no. 2 (2025): e0302352. https://doi.org/10.1371/journal.pone.0302352.

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Bacterial DNA gyrase and topoisomerase IV are the major targets of quinolone antibiotic, and mutational alterations in quinolone resistance determining regions (QRDR) serve as major mechanism of resistance in most bacterial species, including P. aeruginosa. The present investigation was aimed to study the molecular mechanism of fluoroquinolone resistance among clinical P. aeruginosa isolated from Dhaka, Bangladesh, including alterations in target sites of the antimicrobial action. Laboratory collection of 53 P. aeruginosa were subjected to conventional cultural and biochemical characterization
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Mahgoub, Samir A., Shaza Y. A. Qattan, Salma S. Salem, et al. "Characterization and Biodegradation of Phenol by Pseudomonas aeruginosa and Klebsiella variicola Strains Isolated from Sewage Sludge and Their Effect on Soybean Seeds Germination." Molecules 28, no. 3 (2023): 1203. http://dx.doi.org/10.3390/molecules28031203.

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Phenols are very soluble in water; as a result, they can pollute a massive volume of fresh water, wastewater, groundwater, oceans, and soil, negatively affecting plant germination and animal and human health. For the detoxification and bioremediation of phenol in wastewater, phenol biodegradation using novel bacteria isolated from sewage sludge was investigated. Twenty samples from sewage sludge (SS) were collected, and bacteria in SS contents were cultured in the mineral salt agar (MSA) containing phenol (500 mg/L). Twenty colonies (S1 up to S20) were recovered from all the tested SS samples.
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Joshi, Bhavesh M., Mrugesh M. Patel, Rudra B. Parmar, N. K. Singh, and P. V. Tapre. "Molecular Characterization of Plant Growth Promoting Pseudomonas from Rhizosphere of Euphorbia caducifolia Haines." International Journal of Bio-resource and Stress Management 13, no. 11 (2022): 1240–51. http://dx.doi.org/10.23910/1.2022.3262.

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A study was conducted during kharif 2020 at the Department of Microbiology, Chimanbhai Patel College of Agriculture, S. D. Agricultural University for molecular characterization of plant growth promoting Pseudomonas isolates from rhizosphere of Euphorbia caducifolia. Twenty Pseudomonas isolates (designated as PsEc1 to PsEc20) were axenically isolated from rhizospheric soil sample of Euphorbia caducifolia using Pseudomonas Isolation Agar Base (a selective medium). These isolates were tested gram negative, rod shaped, and oxidase positive. All 20 isolates were positive for utilization of maltose
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7

Berebon, Dinebari P., Somtochukwu A. Evurani, Anthony A. Attama, et al. "Antimicrobial profiles of probiotic lactic acid bacteria isolated from Nigerian fermented spice condiment Okpeye (<i>Prosopis africana</i>)." Tropical Journal of Pharmaceutical Research 24, no. 2 (2025): 241–48. https://doi.org/10.4314/tjpr.v24i2.12.

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Purpose: To investigate the antimicrobial profile of lactic acid bacteria (LAB), also called friendly bacteria or probiotics, found in Nigerian fermented spice condiment Okpeye (Prosopis africana). Methods: The LAB was isolated by enrichment and serial dilution. Biochemical characterization of isolates was done using standard microbiological protocols and determined using 16S rDNA gene sequencing. The survival of LAB in low pH 2.5 and 0.3 % bile salt media were evaluated. Using the agar well diffusion assay, the cell-free supernatant (CFS) standardized to 0.5 McFarland was evaluated for antiba
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Buthaina, H. J., and K. A. Ahmed. "Genetic Features of Pseudomonas Aeruginosa Isolated from Skin Infections." Лабораторная диагностика. Восточная Европа 14, no. 1 (2025): 52–62. https://doi.org/10.34883/pi.2025.14.1.004.

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Introduction. The Gram-negative opportunistic pathogen Pseudomonas aeruginosa, is frequently employed in research on virulence and bacterial resistance. It is a source of nosocomial infections including burns, and surgical site infections. Burn and wound injuries disrupt the skin’s natural defenses, allowing P. aeruginosa to colonize more easily and cause infection due to exposure to matrix proteins and inflammatory factors. Purpose. To perform a genetic analysis of P. aeruginosa isolates in various skin samples (burn and wound), from immunocompromised patients in intensive care units in Nasir
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Rawi, Nurul Najihah, Mujahidah Mohd Ramzi, Nor Izzati Abd Rahman, et al. "Antifouling Potential of Ethyl Acetate Extract of Marine Bacteria Pseudomonas aeruginosa Strain RLimb." Life 13, no. 3 (2023): 802. http://dx.doi.org/10.3390/life13030802.

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Biofouling is defined as the excessive colonization process of epibiotic organisms, ranging from microfoulers to macrofoulers, on any submerged surface in water. Previous research has attempted to explore the antifouling activity of bacterial isolates due to the biofouling problems occurring worldwide. One solution is to inhibit the early stage of fouling using secondary metabolites produced by marine bacteria. This study aims to determine the antifouling activities of the marine microorganism P. aeruginosa and to characterize the bacteria isolated as a potential anti-biofouling agent. The bac
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Saidmurad, Mustafa Azm, Sawsan Sajid Al-Jubori та Mohamed Faraj Edbeib. "Prevalence of Metallo β-Lactamase Genes Among MDR Pseudomonas <i>Aeruginosa</i> Isolated from Different Sources in Baghdad". Al-Mustansiriyah Journal of Science 35, № 3 (2024): 48–57. http://dx.doi.org/10.23851/mjs.v35i3.1470.

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Background: Pseudomonas aeruginosa is a gram-negative apportunistic pathogen that has ability to couse different type of infections and charecterised by their high resistance to commonly used antibiotics via different stratiges. Objective: The objective of this study is to detect the prevalence rate of MβL gene among MDR Pseudomonas aeruginosa. Methods: Forty clinical isolates of Pseudomonas aeruginosa were obtained from different hospitials in Baghdad/Iraq. Their identification was confirmed by using 16s rDNA as a housekeeping gene (reference gene). Specific primers were used to detect 3 type
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Book chapters on the topic "16S Rdna Sequencing of P. Aeruginosa"

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Poole Sophie, Singhrao Sim K., Chukkapalli Sasanka, et al. "Active Invasion of Porphyromonas gingivalis and Infection-Induced Complement Activation in ApoE." In Advances in Alzheimer’s Disease. IOS Press, 2017. https://doi.org/10.3233/978-1-61499-706-1-119.

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Periodontal disease is a polymicrobial inflammatory disease that leads to chronic systemic inflammation and direct infiltration of bacteria/bacterial components, which may contribute to the development of Alzheimer's disease. ApoE&amp;minus;/&amp;minus;mice were orally infected (n=12) with Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, and Fusobacterium nucleatum as mono-and polymicrobial infections. ApoE&amp;minus;/&amp;minus;mice were sacrificed following 12 and 24 weeks of chronic infection. Bacterial genomic DNA was isolated from all brain tissues except for the F. nu
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