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Dissertations / Theses on the topic '16S rRNA gen'

Author: Grafiati
Published: 4 June 2021
Last updated: 9 February 2022

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1

Büttner, Christian [Verfasser], and Matthias [Akademischer Betreuer] Noll. "Charakterisierung der anaeroben Gärung verschiedener Ausgangssubstrate mittels 16S rRNA Gen Amplikonsequenzierung und Metaproteomanalytik / Christian Büttner ; Betreuer: Matthias Noll." Bayreuth : Universität Bayreuth, 2021. http://d-nb.info/1236630467/34.

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2

Romero, Condori Pedro Eduardo. "Diversidad y estructura genética de Bostryx scalariformis (Mollusca, Gastropoda) en base a polimorfismos del gen mitocondrial 16S rRNA." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2008. https://hdl.handle.net/20.500.12672/882.

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Bostryx scalariformis (Mollusca, Gastropoda) es un molusco terrestre endémico de las lomas costeras de la costa central del Perú que habita principalmente las zonas arenosas. Sólo se encuentran individuos vivos en las siguientes lomas del departamento de Lima: Ancón, Pasamayo, Lachay y Cerro de Agua. Además, posee morfotipos geográficamente aislados, uno en Ancón-Pasamayo y el otro en Lachay-Cerro de Agua. La fragmentación de su hábitat y la disminución en su tamaño poblacional pueden haber conllevado al decremento en su diversidad genética y una diferenciación genética entre sus poblaciones.
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3

Compte, Port Sergi. "Factors affecting the distribution, abundance and diversity of uncultured archaeal groups in freshwater sediments." Doctoral thesis, Universitat de Girona, 2018. http://hdl.handle.net/10803/663927.

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Archaea are abundant in extreme environments but they are also a prevalent component of microbial communities in soils, marine or freshwater plankton and sediments. Many studies highlighted the prominent role of marine sedimentary Archaea in global nutrient cycles. Less information is available, however, on the diversity, abundance and ecological role of Archaea in freshwater sediments. A serious pitfall for the study of Archaea is their low cultivability under laboratory-controlled conditions thus constraining the progress towards the complete understanding of their metabolic capabilities and
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4

Casas, Reyes Andrea. "Caracterizacion metagenomica asociada a corrosion en un pozo petrolero." Tesis de Licenciatura, Universidad Autónoma del Estado de México, 2018. http://hdl.handle.net/20.500.11799/68483.

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Según los resultados presentados para poder conocer el perfil taxonómico de las muestras de agua obtenidas de un pozo petrolero ubicado en Tabasco utilizando dos distintas plataformas de secuenciación masiva, fue posible conocer mediante dos perspectivas un análisis más completo de la comunidad que se encuentra en dicho pozo, ya que si hubieran sido utilizadas técnicas más básicas de biología molecular e incluso haber recurrido únicamente al uso de técnicas de cultivos bacterianos, no hubiera sido posible conocer más a fondo la comunidad analizada en este trabajo. Por otro lado, los OTUs en
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5

Varna, Klaidas. "Pienarūgščių bakterijų paieška ir jų identifikavimas migruojančių didžiųjų ančių (Anas platyrhynchos) žarnyne naudojant dalinių 16S rRNR geno sekų analizę ir kultivavimu paremtus metodus." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2007~D_20090908_194033-88862.

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Pienarūgščių bakterijų paieška ir jų identifikavimas migruojančių didžiųjų ančių (Anas platyrhynchos) žarnyne naudojant dalinių 16S rRNR geno sekų analizę ir kultivavimu paremtus metodus Klaidas VARNA Vilniaus Universiteto Ekologijos Institutas, Hidrobiontų Ekologijos ir Fiziologijos Laboratorija bei Populiacinės Genetikos Laboratorija, Akademijos-2, Vilnius-21, 08412, Lietuva. Šiame tyrime pavasarinių ir rudeninių didžiųjų ančių (Anas platyrhynchos) migrantų iš Nemuno deltos virškinamojo trakto pieno rūgšties bakterijų įvairovė buvo ištirta naudojant molekulinius metodus (polimerazės grandini
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6

Grešová, Katarína. "Klasifikace bakterií do taxonomických kategorií na základě vlastností 16s rRNA." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2020. http://www.nusl.cz/ntk/nusl-417246.

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The main goal of this thesis was to design and implement a tool that would be able to classify the sequences of the 16S rRNA gene into taxonomic categories using the properties of the 16S rRNA gene. The created tool analyzes all input sequences simultaneously, which differs from common classification approaches, which classify input sequences individually. This tool relies on the fact that bacteria contain several copies of the 16S rRNA gene, which may differ in sequence. The main contribution of this work is design, implementation and evaluation of the capabilities of this tool. Experiments h
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7

Silveira, Érico Leandro da [UNESP]. "Identificação de comunidades bacterianas de solo por seqüenciamento do gene 16S rRNA." Universidade Estadual Paulista (UNESP), 2004. http://hdl.handle.net/11449/94868.

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Made available in DSpace on 2014-06-11T19:27:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2004-04-07Bitstream added on 2014-06-13T19:14:40Z : No. of bitstreams: 1 silveira_el_me_jabo.pdf: 655008 bytes, checksum: ac46de019a2703894b3c1bc37633b43c (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Métodos tradicionais de isolamento e cultivo limitam análises da diversidade microbiana no meio ambiente, pois acredita - se que aproximadamente 10% desses microrganismos possam ser cultivados. A ecologia microbiana molecular teve recentes progressos através da con
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8

Silveira, Érico Leandro da. "Identificação de comunidades bacterianas de solo por seqüenciamento do gene 16S rRNA /." Jaboticabal : [s.n.], 2004. http://hdl.handle.net/11449/94868.

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Resumo: Métodos tradicionais de isolamento e cultivo limitam análises da diversidade microbiana no meio ambiente, pois acredita - se que aproximadamente 10% desses microrganismos possam ser cultivados. A ecologia microbiana molecular teve recentes progressos através da construção de bibliotecas metagenômicas, o que constitui uma poderosa abordagem para explorar a diversidade microbiana de solo fornecendo inclusive dados sobre os microrganismos não cultiváveis desse habitat. Este trabalho teve por objetivo comparar e estimar a diversidade de comunidades bacterianas, em solos de duas áreas, send
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9

Kim, Min Seok. "AN INTEGRATED INVESTIGATION OF RUMINAL MICROBIAL COMMUNITIES USING 16S rRNA GENE-BASED TECHNIQUES." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1316383870.

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10

Thorson, Mary Leah. "Characterization of the structure and function of a Bacteroides thetaiotaomicron 16S rRNA promoter." Thesis, Virginia Tech, 2003. http://hdl.handle.net/10919/42962.

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The bacteroides group is a subdivision in the <I>Cytophaga-Flavobacterium-Bacteroides</I> phylum. This group is as phylogenetically distinct from other Gram-negative enterics, including <I>Escherichia coli</I>, as they are from Gram-positive organisms. Furthermore, there is no cross expression between genes of <I>E. coli</I> and <I>Bacteroides</I> species. It is thought that this difference in gene expression lies in part at the level of transcription initiation and is due to the sequences within the promoter region itself. A putative consensus sequence for <I>Bacteroides</I> promoters has
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11

Balitzki-Korte, Beate. "Speziesidentifizierung mittels vergleichender Sequenzanalyse des mitochondrialen 12S-rRNA-Gens." Diss., lmu, 2004. http://nbn-resolving.de/urn:nbn:de:bvb:19-22998.

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12

Kam, Sin-yee. "Application of 16S rRNA gene sequencing in laboratory diagnosis of mycobacteria other than tuberculosis." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31971052.

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13

Aiemsum-ang, Porntipa. "Isolation, Systematics and Screening of Members of the Streptomyces violaceusniger 16S rRNA Gene Clade." Thesis, University of Newcastle Upon Tyne, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.506611.

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14

金倩儀 and Sin-yee Kam. "Application of 16S rRNA gene sequencing in laboratory diagnosis of mycobacteria other than tuberculosis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31971052.

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15

Duangmal, Kannika. "Systematics and comparative genomics of members of the Streptomyces violaceoruber 16S rRNA gene clade." Thesis, University of Newcastle Upon Tyne, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.403656.

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16

Lanyon, Clare. "16S rRNA gene sequence analysis of non-methanogenic Archaea in a hypereutrophic freshwater lake." Thesis, University of Liverpool, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.402322.

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17

Valešová, Nikola. "Bioinformatický nástroj pro klasifikaci bakterií do taxonomických kategorií na základě sekvence genu 16S rRNA." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2019. http://www.nusl.cz/ntk/nusl-403138.

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Tato práce se zabývá problematikou automatizované klasifikace a rozpoznávání bakterií po získání jejich DNA procesem sekvenování. V rámci této práce je navržena a popsána nová metoda klasifikace založená na základě segmentu 16S rRNA. Představený princip je vytvořen podle stromové struktury taxonomických kategorií a používá známé algoritmy strojového učení pro klasifikaci bakterií do jedné ze tříd na nižší taxonomické úrovni. Součástí práce je dále implementace popsaného algoritmu a vyhodnocení jeho přesnosti predikce. Přesnost klasifikace různých typů klasifikátorů a jejich nastavení je prozko
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18

De, Moors Anick. "Comparison of gene organization in the region that surrounds the 16S rRNA gene in seven different Sulfolobales." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ32533.pdf.

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19

Pereira, Juliana Vianna. "Comparação por análise molecular da diversidade bacteriana da saliva de pacientes com diferentes índices de higiene bucal." Universidade Federal do Amazonas, 2009. http://tede.ufam.edu.br/handle/tede/3064.

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Made available in DSpace on 2015-04-20T12:31:09Z (GMT). No. of bitstreams: 1 Juliana Vianna Pereira.pdf: 4570056 bytes, checksum: c660fca0452deacfa10d23ee9bc79a2b (MD5) Previous issue date: 2009-08-26<br>The complex microbiota of the oral cavity has been intensively studied and saliva is characterized by microorganisms which colonize different regions of mouth, such as tongue, supragengival and subgingival biofilm. Considering this, the purpose of this study was to evaluate the bacterial diversity of the saliva of patients with different levels of oral hygiene according to the Silness,
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20

Hermansson, Anna. "Ammonia-oxidising bacteria in soil : studies of diversity and abundance using 16S rRNA gene analysis /." Linköping : Univ, 2001. http://www.bibl.liu.se/liupubl/disp/disp2001/tek712s.pdf.

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21

Medau, Raphael. "Diversidade de bactérias Burkholderia em solo de Terra Preta Arqueológica da Amazônia por análise em gel de poliacrilamida com gradiente desnaturante (DGGE) e sequenciamento." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-31032008-144434/.

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Dentre os vários microrganismos que fazem parte de microecossistemas de solos, as bactérias do gênero Burkholderia apresentam-se de interesse por possuírem amplo potencial agrícola e biotecnológico. São portadores de uma infinidade de características, como por exemplo: promotoras de crescimento em plantas com a fixação biológica do nitrogênio e produção de fitormônios, supressores de algumas doenças, biorremediadores, agentes de biocontrole e produtores de biopolímeros. Descrito por Yabuuchi et. al. (1992), o gênero ainda precisa ser melhor caracterizado, pois sua taxonomia vem sendo modificad
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Ng, Ho-yin Ricky, and 吳浩然. "Identification of anaerobic, non-sporulating, Gram-positive bacilli from blood cultures by 16S rRNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B44670424.

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23

Kumar, Yashawant. "Mapping metabolites to the phylogeny of strains belonging to the 'Streptomyces violaceusniger' 16S rRNA gene clade." Thesis, University of Newcastle Upon Tyne, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.442218.

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24

Oliveira, Rosangela Claret de. "Isolamento de ureaplasma e micoplasma do trato reprodutivo de ovinos e caprinos e tipificação genotípica por meio da PFGE e seqüenciamento do gene 16S rRNA." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-12012009-105144/.

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Micoplasmas e ureaplasmas têm sido isolados de pequenos ruminantes, mas existem poucos estudos. O Mycoplasma ovine/caprine sorotipo 11, conhecido como cepa 2D, ainda não classificado como espécie, vem sendo relacionado a casos de vulvovaginite e problemas reprodutivos em ovinos e caprinos. Os ureaplasmas apresentam a habilidade em induzirem doenças no trato genital, confirmada por inoculações experimentais de isolados obtidos de animais doentes em animais saudáveis, resultando em moderada granularidade e hiperemia na vulva. Estes ureaplasmas não receberam ainda a designação de espécie, sendo a
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25

Del, Valle Luis J., Michael L. Jaramillo, Miguel Talledo, et al. "Development of a 16S rRNA PCR-RFLP Assay for Bartonella Identification: Applicability in the Identification of Species Involved in Human Infections." Horizon Research Publishing, 2014. http://hdl.handle.net/10757/322342.

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Abstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic
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Winkler, Thorben [Verfasser]. "Das intestinale bakterielle Mikrobiom in der Herzinsuffizienz: eine vergleichende Untersuchung mittels Sequenzierung des 16S rRNA Gens / Thorben Winkler." Kiel : Universitätsbibliothek Kiel, 2019. http://d-nb.info/1182313515/34.

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27

Terceti, Mateus de Souza. "Diversidade bacteriana do gene 16S rRNA em carvão pirogênico de Terra Preta Antropogênica da Amazônia Central e Oriental." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-18122009-143646/.

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A Terra Preta Antropogênica (TPA) tem essa denominação porque é encontrada em sítios arqueológicos, onde viveram grupos pré-históricos e é considerada um dos solos mais férteis do mundo. Nela é encontrada grande quantidade de material deixado por grupos indígenas como fragmentos cerâmicos, artefatos líticos, e especialmente carvão pirogênico. Estudos realizados com o carvão pirogênico verificaram que ele aumenta a capacidade de trocas catiônicas nesses solos. Por meio de microscopia de fluorencência, foi observada a presença de microrganismos habitando esse carvão, no entanto, não se sabe quai
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Pereira, Thays Benites Camargo. "Identificação de diversidade genética em fitoplasmas com base na análise molecular dos gene 16S rRNA, SecY e Proteína Ribossomal." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/11/11135/tde-07012016-175356/.

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Os fitoplasmas são organismos procariotos sem parede celular, que habitam as células do floema das plantas e são transmitidos, principalmente, por cigarrinhas sugadoras de floema. Este patógeno é responsável por causar doenças em diversas espécies vegetais, provocando a expressão de diversos sintomas, entre os quais podem ser destacados a redução do tamanho foliar, amarelecimento das folhas, superbrotamento de ramos e enfezamento da planta hospedeira. Entre os anos de 2013 e 2014, plantas de três espécies vegetais com sintomas característicos de infecção causada por fitoplasmas foram observada
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PAMPLONA, NETO Christóvam. "Filogenia molecular dos xenarthra (Mammalia): análise do grupo cingulata a partir de sequências nucleotídicas do gene mitocondrial rRNA 16S." Universidade Federal do Pará, 2007. http://repositorio.ufpa.br/jspui/handle/2011/4232.

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Submitted by Edisangela Bastos (edisangela@ufpa.br) on 2013-09-05T15:05:32Z No. of bitstreams: 2 license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Dissertacao_FilogeniaMolecularXenarthra.pdf: 988627 bytes, checksum: 1659c92e0cee29ebde40865fb2285a63 (MD5)<br>Approved for entry into archive by Ana Rosa Silva(arosa@ufpa.br) on 2013-09-10T13:28:57Z (GMT) No. of bitstreams: 2 license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Dissertacao_FilogeniaMolecularXenarthra.pdf: 988627 bytes, checksum: 1659c92e0cee29ebde40865fb2285a63 (MD5)<br>Made avail
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Al, Masalma Mouhamad. "Molecular and cultural analysis of the bacterial flora associated with brain abscesses." Thesis, Aix-Marseille 2, 2011. http://www.theses.fr/2011AIX20663/document.

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Les abcès cérébraux sont des infections potentiellement mortelles, entraînant souvent des séquelles graves. La prise en charge médicale en reste empirique en raison d’un manque de connaissance approfondie des microorganismes responsables de cette condition. Dans la plupart des laboratoires microbiologiques, le diagnostic d’abcès cérébral est basé sur la culture du pus recueilli chirurgicalement. Malheureusement, cette procédure a de nombreuses limites et ne permet l’identification que d’une petite partie de la population microbienne en cause. L’amplification par PCR et le séquençage du gène co
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Yamaguti, Mauricio. "Isolamento de micoplasma de suínos com problemas respiratórios e tipificação dos isolados pela PFGE e seqüenciamento do gene 16S rRNA." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-29102009-091226/.

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As doenças respiratórias são responsáveis, na suinocultura, por grandes perdas econômicas e entre os agentes destacam-se os micoplasmas. Foram coletadas 126 amostras de muco nasal/tonsilar, e fragmentos de 78 pulmões, 2 de traquéia e 2 de tonsila. No isolamento foi utilizado o meio FRIIS modificado, na identificação, a Multiplex-PCR e na tipificação, a PFGE e sequenciamento do gene 16S rRNA. Foram obtidos 59 isolados identificados como M. hyopneumoniae (1,70%), M. flocculare (3,40%) e M. hyorhinis (94,90%). A PFGE dos isolados de M. hyorhinis, resultou em 10 e 9 perfis com a enzima AvaI e XhoI
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Yeung, Shiu-yan, and 楊兆恩. "Update and evaluation of 16SpathDB, an automated comprehensive database for identification of medically important bacteria by 16S rRNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/193552.

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Identification of pathogens is one of the important duties of clinical microbiology laboratory. Traditionally, phenotypic tests are used to identify the bacteria. However, due to some limitations of the phenotypic tests, the bacteria may not be identified sometimes and cannot be identified promptly. 16S rRNA gene sequencing is a rapid and accurate method to achieve this target. It is especially useful for identify rare or slow growing bacteria. However, the interpretation of the 16S rRNA gene sequencing result is one of the challenging duties to laboratory technicians and microbiologists. Apar
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Fisher, Marc Lewis. "Comparison of Subterranean Termite (Rhinotermitidae: Reticulitermes) Gut Bacterial Diversity Within and Between Colonies and to Other Termite Species Using Molecular Techniques (ARDRA and 16S rRNA Gene Sequencing)." Diss., Virginia Tech, 2006. http://hdl.handle.net/10919/27379.

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Termites are known to harbor within their gut a diverse assemblage of symbiotic microorganisms. Little work has been done, however, to describe the diversity and function of the bacteria in the economically important eastern subterranean termite, Reticulitermes flavipes. The first object of this study was to characterize the bacterial diversity in the gut of R. flavipes using amplified rDNA restriction analysis (ARDRA) and 16S rRNA gene sequencing. It was determined that ARDRA was an effective technique for characterizing the diversity of the termite gut microbiota. Of the 512 clones analyz
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Frolovienė, Jurga. "Šunų babeziozės sukėlėjo Babesia canis 18S rRNR geno sekų palyginamoji analizė." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140611_143551-28885.

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Šunų babeziozė – tai liga, greitai plintanti visame pasaulyje, kurią sukelia pirmuonys, priklausantys Babesia genčiai. Ligos sukėlėjus platina Dermocentor, Rhipicephalus, Haemaphysalis genties erkės. Lietuvoje paplitę Dermacentor reticulatus erkės - pagrindiniai Babesia canis vektoriai. B. canis patenka į raudonąsias kraujo ląsteles (eritrocitus) ir jas suardo. Kasmet sergamumas šunų babezioze visoje Europoje bei Lietuvoje stipriai auga. Darbo tikslas buvo įvertinti šunų užsikrėtimą Babesia canis patogenais ir identifikuoti Babesia canis porūšius ir padermes, panaudojant molekulinius tyrimo me
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Calus, Szymon Tomasz. "Evaluation of nanopore-based sequencing technology for gene marker based analysis of complex microbial communities : method development for accurate 16S rRNA gene amplicon sequencing." Thesis, University of Glasgow, 2018. http://theses.gla.ac.uk/41086/.

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Nucleic acid sequencing can provide a detailed overview of microbial communities in comparison with standard plate-culture methods. Expansion of high-throughput sequencing (HTS) technologies and reduction in analysis costs has allowed for detailed exploration of various habitats with use of amplicon, metagenomics, and metatranscriptomics approaches. However, due to a capital cost of HTS platforms and requirements for batch analysis, genomics-based studies are still not being used as a standard method for the comprehensive examination of environmental or clinical samples for microbial character
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Renvoisé, Aurélie. "Applicabilité de la PCR "universelle" 16S comme outil d'identification et de détection bactérienne en laboratoire hospitalier de bactériologie." Thesis, Aix-Marseille, 2012. http://www.theses.fr/2012AIXM5016/document.

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La PCR universelle ciblant le gène codant pour l'ARNr 16S à l'aide d'amorces universelles, a d'abord été développée pour des études phylogénétiques. En effet ce gène est universellement retrouvé chez les bactéries et sa fonction est conservée. Ainsi, il peut servir d'« horloge moléculaire » pour mesurer les distances phylogénétiques entre les différentes espèces bactériennes. La PCR universelle a ensuite été appliquée en microbiologie clinique dans deux domaines distincts : la détection et l'identification bactériennes. Dans ce travail, nous avons évalué l'applicabilité de la PCR « universelle
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Thi, Tuyen Do, Quyen Le Dinh, Thi Quyen Dinh, and Cuong Pham Van. "Isolation and identification of marine bacteria from marine mud in Vietnam with antimicrobial activity." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-99508.

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Seventeen bacterial strains were isolated from 9 marine mud samples from the inshore environments of the East Sea. Four bacterial strains showed an inhibition against all tested microorganisms Staphylococcus aureus ATCC10832, Escherichia coli JM109, and Fusarium oxysporum. 16S rRNA sequences of four bacterial strains were obtained by PCR using specific primers. PCR products were cloned into E. coli DH5a using pJET1.2 blunt vector. The recombinant plasmids were sequenced and the lengths of these 16S rRNA sequences were ~930bp. The 16S rRNA sequence from the four bacterial DB1.2, DB1.2.3, DB4.2
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Sánchez, Arroyo Alicia 1989. "Metabarcoding of the 18S rRNA gene to uncover new molecular biodiversity in Metazoa and unicellular Opisthokonta." Doctoral thesis, Universitat Pompeu Fabra, 2019. http://hdl.handle.net/10803/667270.

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One of the main evolutionary transitions is the origin of animal and fungal multicellularity from single-cell ancestors. To address this change, it is necessary to study the extant unicellular relatives of animals (Metazoa) and fungi, all of them forming the Opisthokonta clade. Recent data have shown that adding more taxa to the Opisthokonta tree can dramatically change our view on this transition. However, the real diversity of these organisms is mostly unknown. Therefore, the main goal of this Ph.D. was to analyse the molecular diversity of both microbial opisthokonts as well as animals, sea
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39

Souza, Roberto Antonio de. "Identificação de linhagens atípicas de Yersinia spp. por métodos moleculares." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/60/60135/tde-26052009-161112/.

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O gênero Yersinia compreende 12 espécies. Y. enterocolitica, Y. pseudotuberculosis e Y. pestis são patógenos de vários animais, incluindo os humanos. Y. aldovae, Y. bercovieri, Y. frederiksenii, Y. intermedia, Y. kristensenii, Y. aleksiciae, Y. mollaretti e Y. rhodei são encontradas sobretudo no meio ambiente e alimentos e consideradas, usualmente, como bactérias oportunistas não-patogênicas e Y. ruckeri é um importante patógeno de peixes. Usualmente, as linhagens de Yersinia são classificadas em espécies de acordo com suas características bioquímicas. O Laboratório Nacional de Referência em Y
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Costa, Poliene Martins. "Caracterização da comunidade bacteriana associada ao trato intestinal de Spodoptera frugiperda provenientes de diferentes dietas." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-26042016-104030/.

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A importância da praga Spodoptera frugiperda (Lepidoptera: Noctuidae) deve-se não somente aos danos provocados às lavouras de milho, mas a capacidade de se alimentar de uma ampla variedade de famílias de plantas. Lagartas desta espécie são capazes de se adaptar a dietas contendo inibidores de peptidase de soja (IPS). Há uma hipótese de que a microbiota intestinal neste inseto poderia estar envolvida com estes mecanismos de adaptação. Neste contexto, um dos objetivos do trabalho foi verificar se estas bactérias poderiam alterar a expressão gênica de serino peptidases e atividade enzimática nest
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Dobbler, Priscila Caroline Thiago. "Baixa diversidade e sucessão microbiana anormal estão associadas à enterocolite necrosante em recém-nascidos prematuros." Universidade Federal do Pampa, 2017. http://dspace.unipampa.edu.br:8080/xmlui/handle/riu/1608.

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Submitted by Ana Damasceno (ana.damasceno@unipampa.edu.br) on 2017-06-07T18:12:48Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Baixa diversidade e sucessão microbiana anormal estão associadas à enterocolite necrosante em recém-nascidos prematuros.pdf: 1587508 bytes, checksum: c407e4cf94f25b2272a7d25213f72873 (MD5)<br>Made available in DSpace on 2017-06-07T18:12:48Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Baixa diversidade e sucessão microbiana anormal estão associadas à enteroc
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Wright, André-Denis G. "Molecular phylogeny of the endosymbiotic ciliates, Litostomatea, trichostomatia, of vertebrate animals inferred from 18s rRNA gene sequences." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/NQ31910.pdf.

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43

Garcia, Carlos Eduardo. "Isolamento e identificação de actinobacterias em solos de terra preta antropogenica (TPA) da Amazonia Central por ARDRA e sequenciamento do gene 16S rRNA." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256616.

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Orientadores: Fumio Yokota, Tasi Siu Mui<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos<br>Made available in DSpace on 2018-08-05T16:40:59Z (GMT). No. of bitstreams: 1 Garcia_CarlosEduardo_D.pdf: 3424911 bytes, checksum: 6338d2ede1846761521ec0cfb4c5e1ec (MD5) Previous issue date: 2006<br>Doutorado<br>Doutor em Ciência de Alimentos
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Nobrega, Letícia Maria Menezes 1983. "Estudo da diversidade bacteriana de canais radiculares infectados em casos de abscesso apical agudo por cultura, clonagem e sequenciamento do gene 16S rRNA." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290434.

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Orientador: Brenda Paula Figueiredo de Almeida Gomes<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba<br>Made available in DSpace on 2018-08-22T06:43:53Z (GMT). No. of bitstreams: 1 Nobrega_LeticiaMariaMenezes_D.pdf: 2252800 bytes, checksum: 31910f0e934110c7e863efa8d0e293da (MD5) Previous issue date: 2012<br>Resumo: Abscessos apicais agudos são condições frequentes na urgência endodôntica. São infecções polimicrobianas que apresentam variações entre indivíduos, e afetam o sistema de canais radiculares e tecidos periapicais. Associações bacterian
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Rezende, Lilian Ribeiro. "Caracterização molecular da comunidade bacteriana em rebanhos leiteiros com mastite subclínica." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11139/tde-10082016-130206/.

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A mastite bovina é considerada a doença de maior impacto nos rebanhos leiteiros, exercendo efeito econômico negativo sobre a produtividade e perdas significativas à indústria de laticínios. Tendo em vista os impactos na sanidade animal e os prejuízos econômicos acarretados, o objetivo deste estudo foi caracterizar de forma mais abrangente a comunidade microbiana presente em rebanhos leiteiros com mastite subclínica utilizado o sequenciamento parcial do gene 16S ribossomal RNA (rRNA). Especificamente, foram caracterizadas as comunidades bacterianas presentes em amostras de leite vindas de três
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Wakeman, Jane A. "Human ribosomal RNA : primary structure analysis of the 28S rRNA gene and preliminary studies on the distribution of pseudouridine residues in the 18S and 28S rRNA molecules." Thesis, University of Liverpool, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317264.

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Mestrinelli, Fabiana. "Isolamento e caracterização de microrganismo envolvido na desnitrificação autrófica pela oxidação de sulfeto em reator vertical de leito fixo." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/18/18138/tde-19112010-150533/.

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O presente estudo teve como objetivo avaliar a comunidade envolvida na desnitrificação autotrófica pela oxidação de sulfetos, aplicada ao pós-tratamento de efluentes anaeróbios. O enriquecimento da comunidade bacteriana e da comunidade desnitrificante autotrófica foi realizado a partir de amostras da biomassa coletadas de três reatores verticais de leito fixo operados em condições distintas, sendo, redução autotrófica de nitrato, redução autotrófica de nitrito e redução autotrófica de nitrato com excesso de sulfeto. Após a determinação da melhor condição de enriquecimento, a cultura foi purifi
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48

Mulheron, Rebecca. "Microbial Community Assembly found with Sponge Orange Band Disease in Xestospongia muta (Giant Barrel Sponge)." NSUWorks, 2014. http://nsuworks.nova.edu/occ_stuetd/18.

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The giant barrel sponge, Xestospongia muta is an iconic and essential species of the coral reefs in South Florida. The sponge has primary roles providing ecosystem services and creating unique habitats for diverse microbial communities. On April 27, 2012 an outbreak of Sponge Orange Band Disease (SOB) was detected off the coast of South Florida. The disease begins with sponge bleaching, followed by mesohyl or “mesohyl” necrosis and often total mesohyl disintegration. Sampling from two diseased populations at Boynton Beach and Fort Lauderdale, FL took place on May 11th and May 29th, 2012. Each
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49

Erram, Dinesh. "Characterization of the larval habitat of Culicoides sonorensis (Diptera: Ceratopogonidae) with emphasis on the significance of animal manure and the associated bacterial community." Diss., Kansas State University, 2016. http://hdl.handle.net/2097/32573.

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Doctor of Philosophy<br>Department of Entomology<br>Ludek Zurek<br>The larval stages of Culicoides sonorensis Wirth and Jones, a confirmed vector of bluetongue and epizootic hemorrhagic disease viruses affecting ruminants in North America, have been observed to occur typically in animal waste enhanced muds. In this dissertation, I studied the larval development (first instar to adult stage) and oviposition (four-choice assays) of C. sonorensis on sterilized mud (autoclaved) enriched with manure of different farm animal species (dairy cattle, beef cattle, sheep, goats, pigs, horses, white-taile
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Cersosimo, Laura M., Melissa L. Bainbridge, Jana Kraft, and André-Denis G. Wright. "Influence of periparturient and postpartum diets on rumen methanogen communities in three breeds of primiparous dairy cows." BIOMED CENTRAL LTD, 2016. http://hdl.handle.net/10150/614742.

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Background: Enteric methane from rumen methanogens is responsible for 25.9 % of total methane emissions in the United States. Rumen methanogens also contribute to decreased animal feed efficiency. For methane mitigation strategies to be successful, it is important to establish which factors influence the rumen methanogen community and rumen volatile fatty acids (VFA). In the present study, we used next-generation sequencing to determine if dairy breed and/or days in milk (DIM) (high-fiber periparturient versus high-starch postpartum diets) affect the rumen environment and methanogen community
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