Relevant bibliographies by topics / 1D SDS-PAGE

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic '1D SDS-PAGE'

Author: Grafiati
Published: 4 June 2021
Last updated: 18 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic '1D SDS-PAGE.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "1D SDS-PAGE"

1

Oliveros, Carolina Vega, Carolina Chegwin Angarita, and Harold Dubán Ardila Barrantes. "Condiciones para el análisis de proteínas del micelio de Lentinula edodes obtenido por fermentación en estado líquido." Revista Colombiana de Química 48, no. 3 (2019): 3–12. http://dx.doi.org/10.15446/rev.colomb.quim.v48n3.74843.

Full text
Abstract:
Lentinula edodes es una seta comestible con potencial para el desarrollo de nutraceúticos. Sin embargo, son incipientes los trabajos enfocados en su producción biotecnológica y el desarrollo de herramientas analíticas que permitan profundizar en su composición. En esta investigación se estudió la producción de biomasa del hongo en el tiempo mediante fermentación en estado líquido y se seleccionaron las condiciones que permiten la obtención de extractos para la aplicación de herramientas para análisis proteómicos. Los métodos de extracción de proteínas, ácido tricloroacético (TCA)-Acetona y TCA-Acetona-Fenol, fueron comparados en términos del rendimiento de extracción y los perfiles de separación usando electroforesis en 1D (SDS-PAGE) y 2D (IEF-SDS PAGE). Se determinó que a los 10 días de crecimiento se obtiene la mayor producción de biomasa y proteína total. La extracción con TCA-Acetona-Fenol presentó un mayor rendimiento, mayor resolución y número de bandas en la electroforesis 1D. En 2DE los dos métodos permitieron la extracción de proteínas con puntos isoeléctricos en el rango de pH 3-10, pero el método TCA-Acetona-Fenol conllevó a una extracción diferencial, favoreciendo el rango de 33 a 113 kDa. Estos resultados se constituyen en una primera aplicación de técnicas de separación electroforética para futuros estudios proteómicos
APA, Harvard, Vancouver, ISO, and other styles
2

Kottahachchi, D. U., T. R. Ariyaratne, and G. A. U. Jayasekera. "Mass Spectrometry Based Analysis of Erythrocyte Membrane Associated Proteins in Chronic Myeloid Leukemia Patients in Sri Lanka." International Letters of Chemistry, Physics and Astronomy 38 (September 2014): 74–86. http://dx.doi.org/10.18052/www.scipress.com/ilcpa.38.74.

Full text
Abstract:
The research reported in this paper was conducted to analyze erythrocyte membrane associated proteins (ERMBPs) of some of chronic myeloid leukemia (CML) patients and selected individuals of Sri Lanka employing one dimensional sodium dodecyl sulphate poly acrylamide gel electrophoresis (1D-SDS-PAGE) combined with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI–TOF-MS). Erythrocyte membranes from blood were isolated by osmotic lysis, centrifugation and washings. ERMBPs were separated on 1D-SDS-PAGE, visualized by silver staining and the separated protein bands dissected out from the gel and were subjected to digestion by proteolytic enzyme, trypsin, and the resulting peptide mixture was analyzed by MALDI-TOF-MS. Resulting experimental peptide mass values were analyzed by peptide mass fingerprint (PMF) technique. From this analysis 10 ERMBPs including α and β spectrin, ankyrin, band 3, band 4.1, band 4.2, band 7, dematin, actin, 55 KDa erythrocyte membrane protein were identified accurately with their primary structure information. The study was able to provide some evidence for Cathepsin associated cleavage of Band 3 anion transport protein in CML patients reported previously. In addition we were able to detect changes in gel bands between healthy controls and CML patients around the area of 20 kDa in the 1 D-SDS-PAGE. It was identified as nuclear protein Dbf 4 related factor 1 isoform 2. Although erythrocytes are devoid of nuclei, such unexpected nuclear proteins have been identified in previous research. We were successful in identifying several human ERMBPs with available resources. As the identified proteins were known to be related to pathology of some of the hematological diseases, this methodology could be extended to detect the protein changes in erythrocyte membrane protein associated diseases. Therefore, this initial research would at some point lead to discovery of biomarkers to these hematological diseases in Sri Lanka.
APA, Harvard, Vancouver, ISO, and other styles
3

Lawrence, Gary W., and J. Oliver Dolly. "Multiple forms of SNARE complexes in exocytosis from chromaffin cells: effects of Ca2+, MgATP and botulinum toxin type A." Journal of Cell Science 115, no. 3 (2002): 667–73. http://dx.doi.org/10.1242/jcs.115.3.667.

Full text
Abstract:
The changes that SNAREs undergo during exocytosis were studied in permeabilised chromaffin cells treated with Ca2+, MgATP or botulinum neurotoxin A. High-resolution 2D SDS-PAGE revealed multiple SDS-resistant SNARE complexes having a wide range of sizes and in which SNAP-25 and syntaxin predominate over synaptobrevin. Their formation increased upon Ca2+-stimulated exocytosis; notably, the 2D protocol proved much superior to 1D SDS-PAGE for the detection of large complexes and revealed that for forms with relative molecular mass greater than 100,000 stimulated induction was more significant than for smaller species. MgATP enhanced Ca2+-triggered catecholamine release but reduced the content of complexes. By contrast, botulinum neurotoxin type A inhibited exocytosis and altered the stoichiometry of the SNAP-25:syntaxin binary association, without lowering its abundance. The individual SNAREs were protected against trypsin proteolysis to varying extents in binary and ternary complexes of different sizes, suggestive of distinct folding intermediates. Our data suggest that Ca2+ triggers an early stage of SNARE complex formation causing an accumulation of partially folded intermediates, especially of binary forms, as well as their maturation into smaller, more protease resistant states. In addition, botulinum neurotoxin A inhibits exocytosis by perturbing the syntaxin:SNAP-25 ratio in binary intermediates.
APA, Harvard, Vancouver, ISO, and other styles
4

Dotlačil, L., E. Gregová, J. Hermuth, Z. Stehno, and J. Kraic. "Diversity of HMW-Glu Alleles and Evaluation of their Effects on some Characters in Winter Wheat Landraces and Old Cultivars." Czech Journal of Genetics and Plant Breeding 38, No. 3-4 (2012): 109–16. http://dx.doi.org/10.17221/6244-cjgpb.

Full text
Abstract:
Earliness, morphological and agronomic characters and grain quality were studied in 123 European landraces and old cultivars of winter wheat in three-year field experiments. Simultaneously, HMW Glu-alleles were identified in these cultivars by means of SDS-PAGE. Within this set of cultivars 224 Glu-lines (with occurrence over 5% in the cultivar) were identified carrying 3 different allelic combinations at 1A, 10 combinations at 1B and 3 combinations at 1D chromosomes, respectively. Relatively rare were alleles 2* at 1A and 3+12 at 1D as well as alleles 8, 6, 9, 7, 13+16 and 17+ 18 at 1B. Allele 20 at 1B was identified only in cultivars from DNK, CHE and EST. Allele 2* at 1A locus was found mainly in cultivars from Eastern, South-Eastern and Central Europe. Allelic combination 17+18 at 1B was also characteristic of cultivars from Central Europe. However, the gluten patterns themselves were not a sufficient tool for geographic characterisation of cultivars. The composition of Glu-alleles influenced the earliness of cultivars (alleles 2* at 1A, 17+ 18 and 6 at 1B and 3+12 at 1D). Spike length was positively affected by allele 1 at 1A and number of spikelets per spike by alleles 2+12 et 1D chromosome. Allele 2* was also associated with lower grain weight per spike. Crude protein content was decreased in cultivars where GS at 1A locus was absent (0). The value of gluten index was considerably higher (59.2) in cultivars bearing allelic combination 5+10 at 1D. A number of alleles affected the values of SDS micro-sedimentation test.
APA, Harvard, Vancouver, ISO, and other styles
5

Cathro, Peter, Peter McCarthy, Peter Hoffmann, and Peter Zilm. "Isolation and identification of Enterococcus faecalis membrane proteins using membrane shaving, 1D SDS/PAGE, and mass spectrometry." FEBS Open Bio 6, no. 6 (2016): 586–93. http://dx.doi.org/10.1002/2211-5463.12075.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Marin, Frédéric, Françoise Immel, Nolwenn Trinkler, and Danièle Gaspard. "Staining SDS-PAGE Gels of Skeletal Matrices after Western Blot: A Way to Improve their Sharpness." Key Engineering Materials 672 (January 2016): 215–21. http://dx.doi.org/10.4028/www.scientific.net/kem.672.215.

Full text
Abstract:
Denaturing 1D electrophoresis on acrylamide gels, also referred as SDS-PAGE, is a classical technique for fractionating and visualizing the macromolecular constituents of matrices associated to calcified tissues. This technique has been widely used in association with the subsequent silver nitrate staining. But because matrices associated to calcified tissues are very often glycosylated and constituted of numerous polydisperse macromolecules, the obtained pattern is frequently ‘smeary’ and discrete bands, when present on the gel, are often blurred and thickened. In this paper, we present a simple protocol that can circumvent this drawback and ‘clean’ the gels. In short, after the classical migration step of the matrix macromolecules, the gel is electro-blotted on a PVDF membrane, similarly to a Western blot, but for a shorter time (partial transfer, i.e., one hour or less). It is subsequently stained with silver nitrate. The likely effect of the transfer is to partly remove polydisperse macromolecules and to ‘sharpen’ the discrete bands. We think that this extra-step may improve in several cases the gel pictures, particularly when they are blurred. We illustrate this phenomenon with two examples taken from brachiopod and mollusc shell matrices.
APA, Harvard, Vancouver, ISO, and other styles
7

Yang, F. P., L. H. Wang, J. W. Wang, et al. "Characterisation of high- and low-molecular-weight glutenin subunit genes in Chinese winter wheat cultivars and advanced lines using allele-specific markers and SDS-PAGE." Crop and Pasture Science 61, no. 1 (2010): 84. http://dx.doi.org/10.1071/cp09164.

Full text
Abstract:
Wheat end-use product quality is highly influenced by the composition and quantity of high- and low-molecular-weight glutenin subunits (HMW-GS and LMW-GS). In the present study, 224 Chinese wheat cultivars and advanced lines were characterised for the HMW-GS and LMW-GS with allele-specific PCR markers and sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that 56 cultivars (25.0%) carried the allele Glu-D1-1d (Dx5), while 80 cultivars (35.7%) with the allele Glu-B1-2a (By8) produced a 527-bp specific band. Fourteen genotypes (6.3%) with the allele Glu-B1e (Bx20) yielded a 701-bp amplicon with the marker Mar and a 753-bp specific PCR fragment with the marker ZSBy9aF1/R3. Glu-B1h (Bx14+By15) was present in only 1 genotype, and 2 cultivars contained the allele Glu-B1f (Bx13+By16) identified with the marker ZSBy9F2/R2. Four genotypes (1.8%) with the allele Glu-B1-1d (Bx6) gave 695-bp and 830-bp bands, and 5 genotypes (2.2%) with the allele Glu-B1i (Bx17+By18) amplified a 659-bp fragment using the marker Bx. One hundred and six cultivars (47.3%) had the allele Glu-B1-2b (By9), amplifying a 663-bp fragment with the marker ZSBy9aF1/R3; 34 genotypes (15.8%) contained the allele Glu-B3d, generating a 662-bp PCR fragment with the marker gluB3d. Fifteen cultivars (7.0%) with the allele Glu-B3b yielded 1570-bp and 750-bp PCR amplicons with the markers gluB3b and gluB3bef, respectively. The allele Glu-B3h was found in 7 cultivars, generating a 1022-bp PCR fragment with the marker gluB3h. The genotypes detected by SDS-PAGE were mostly consistent with those identified by molecular markers, confirming the utility of the molecular markers. The information for the HMW-GS and LMW-GS in Chinese wheat cultivars will be useful in wheat breeding programs.
APA, Harvard, Vancouver, ISO, and other styles
8

Burgess, Treena, Nicholas Malajczuk, and Bernard Dell. "Variation in Pisolithus based on basidiome and basidiospore morphology, culture characteristics and analysis of polypeptides using 1D SDS-PAGE." Mycological Research 99, no. 1 (1995): 1–13. http://dx.doi.org/10.1016/s0953-7562(09)80309-2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Yao, Hui-peng, Lin Chen, Xingwei Xiang, Ai-qin Guo, Xing-meng Lu, and Xiao-feng Wu. "Proteomics identification and annotation of proteins of a cell line of Bombyx mori, BmN cells." Bioscience Reports 30, no. 3 (2010): 209–15. http://dx.doi.org/10.1042/bsr20090045.

Full text
Abstract:
A cell line is an important experimental platform for biological sciences as it can basically reflect the biology of its original organism. In this study, we firstly characterized the proteome of cultured BmN cells, derived from Bombyx mori. Total 1478 proteins were identified with two or more peptides by using 1D (one-dimensional) SDS/PAGE and LTQ-Orbitrap. According to the gene ontology annotation, these proteins presented diverse pI values and molecular masses, involved in various molecular functions, including catalytic activity, binding, molecular transducer activity, motor activity, transcription regulator activity, enzyme regulator activity and antioxidant activity. Some proteins related to virus infection were also identified. These results provided us with useful information to understand the molecular mechanism of B. mori as well as antiviral immunity.
APA, Harvard, Vancouver, ISO, and other styles
10

Wöhlbrand, Lars, Hanna S. Ruppersberg, Christoph Feenders, Bernd Blasius, Hans-Peter Braun, and Ralf Rabus. "Analysis of membrane-protein complexes of the marine sulfate reducer Desulfobacula toluolica Tol2 by 1D blue native-PAGE complexome profiling and 2D blue native-/SDS-PAGE." PROTEOMICS 16, no. 6 (2016): 973–88. http://dx.doi.org/10.1002/pmic.201500360.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "1D SDS-PAGE"

1

Mofokeng, Henrietta Refiloe. "Identification of a transducin (beta)-like 3 protein as a potential biomarker of prediabetes from rat urine using proteomics." Thesis, University of the Western Cape, 2010. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_4144_1361870602.

Full text
Abstract:
<p>Obesity is a globally increasing disease particularly in developing countries and among children. It is mainly caused by intake of diets high in fat and the lack of physical activity. Obesity is a risk factor for diseases such as type II diabetes, high blood pressure, high cholesterol and certain cancers. Prediabetes is a condition where blood glucose levels are above normal but have not&nbsp<br>reached those of diabetes. It is difficult to diagnose, as there are no signs or symptoms. Some type II diabetes patients bear no symptoms at all and the disease is discovered late. Proteomics is a field that can provide opportunities for early diagnosis of diseases through biomarker discovery. The early diagnosis of diabetes can assist in the prevention and treatment of diabetes. Therefore there is a need for the early diagnosis of diabetes. Twenty Wistar rats were used. The rats were initially fed a CHOW diet, which is the standard balanced diet for rats, for 4 weeks. The rats were then divided into 2 groups of 10 where 1 group was fed CHOW and another was fed a high fat (HF) diet in order to induce obesity. The two groups were fed their respective diets for 18 weeks. Rats were weighed. Rats were placed in metabolic chambers and 24 hour urine samples were collected. Ketone levels were measured by Ketostix. Urine proteins were precipitated by acetone, quantified and separated on both the 1D SDS-PAGE and the 2D SDS-PAGE. Protein expression changes between CHOW and HF fed rats were determined and identified using MALDI-TOF mass spectrometry. Protein spots intensities increased and decreased between the CHOW and HF fed rats. Transducin (beta)-like 3 was identified as the only differentially expressed protein, which might serve as a potential biomarker for prediabetes.</p>
APA, Harvard, Vancouver, ISO, and other styles
2

Cakici, Ozgur. "Biochemical And Genetic Characterization Of Halobacterium Salinarium Strain Isolated From Tuz Lake In Central Anatolia." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/3/12604752/index.pdf.

Full text
Abstract:
In this study, a halophilic archaea Halobacterium salinarium TG13 which is isolated from Tuz Lake in Central Anatolia was characterized biochemically and genetically. Halobacterium salinarium DSM3754 and Halobacterium salinarium S9 strains were used as a reference strain through the experiments. In biochemical characterization<br>total protein profiles of strains was compared by using 1D SDS PAGE. Total protein profile of the isolated strain has shown differences. The SDS-PAGE profile of the purified purple membrane showed only single band by coomassie staining. Molecular weight and pI values of the protein isolated from Halobacterium salinarium TG13 and Halobacterium salinarium S9 were estimated by 2D SDS-PAGE as 22 kD and 5.4, respectively. Photoactivity of purple membrane of the strains was investigated. pH change of the purple membranes were observed upon illumination. This protein might be corresponded to bacteriorhodopsin. In genetical characterization<br>polymorphism of genomic DNA of strains was scanned with RAPD-PCR. Plasmid DNA profiles of strains was determined to make use of RFLP technique. RAPD-PCR and RFLP analyses have shown that Halobacterium salinarium TG13 is different strain from reference Halobacterium salinarium strains (H.s. S9 and H.s. DSM3754).
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "1D SDS-PAGE"

1

Prieto, DaRue A., Gordon Whitely, Donald J. Johann, and Josip Blonder. "Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7558-7_4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Brunelle, Julie L., and Rachel Green. "One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)." In Methods in Enzymology. Elsevier, 2014. http://dx.doi.org/10.1016/b978-0-12-420119-4.00012-4.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "1D SDS-PAGE"

1

Ramaswamy, Gomathy, Bing Wu, and Ursula MacEvilly. "CODES: A computerised approach to the analysis and management of 1D SDS PAGE gel protein image information." In 2009 Second International Conference on the Applications of Digital Information and Web Technologies (ICADIWT). IEEE, 2009. http://dx.doi.org/10.1109/icadiwt.2009.5273881.

Full text
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic '1D SDS-PAGE' for particular source types:
Journal articles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography