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Journal articles on the topic '2-mercaptoethanol'

Author: Grafiati

Published: 4 June 2021

Last updated: 17 June 2025

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1

Lal, Manohar, and David A. Armstrong. "The radiation chemistry of 2-mercaptoethanol." Canadian Journal of Chemistry 63, no. 1 (1985): 30–32. http://dx.doi.org/10.1139/v85-005.

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Hydrogen, hydrogen sulphide, mercaptoethanol disulphide, and ethanol yields from the radiolysis of 2-mercaptoethanol are consistent with the same mechanism postulated for other sulphydryl-containing molecules. In all cases studied gH in neutral solution appears to be enhanced (gH = 0.7–0.9 molecule per 100 eV). However, there is no basis for attributing this to reaction [10] which was postulated in earlier work:[Formula: see text]but is not supported by independent data from H atom studies.

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2

Casassas, E., C. Ariño, and M. Esteban. "Cathodic stripping voltammetry of 2-mercaptoethanol." Analytica Chimica Acta 176 (1985): 113–19. http://dx.doi.org/10.1016/s0003-2670(00)81638-4.

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3

González, S., P. Carro, A. Hdez Creus, and A. Arévalo. "Adsorption of 2-mercaptoethanol and In(III)-2-mercaptoethanol on mercury electrodes in HClO4 + NaClO4 medium." Journal of Electroanalytical Chemistry and Interfacial Electrochemistry 238, no. 1-2 (1987): 367–81. http://dx.doi.org/10.1016/0022-0728(87)85186-0.

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4

BUTSUGAN, Hitonari, Hiroaki SHIMIZU, Nobuyuki NAKAYAMA, and Fujito NEMOTO. "Reaction of perfluoro(2-methyl-2-pentene) with 2-mercaptoethanol." NIPPON KAGAKU KAISHI, no. 10 (1989): 1772–77. http://dx.doi.org/10.1246/nikkashi.1989.1772.

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5

Afşar, Hüseyin, Reşat Apak, and İzzet Tor. "Spectrophotometric determination of selenium with 2-mercaptoethanol." Analyst 114, no. 10 (1989): 1319–21. http://dx.doi.org/10.1039/an9891401319.

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6

Sperker, S., G. Gsaller, H. Marek, and G. Gritzner. "Solvent properties of ethanediol and 2-mercaptoethanol." Journal of Solution Chemistry 17, no. 1 (1988): 35–46. http://dx.doi.org/10.1007/bf00651851.

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7

Paul-Pletzer, Kalanethee, and Jerome Parness. "Elimination of Keratin Contaminant from 2-Mercaptoethanol." Analytical Biochemistry 289, no. 1 (2001): 98–99. http://dx.doi.org/10.1006/abio.2000.4949.

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8

KIMURA, MUTSUMI, YUJI YAMAGUCHI, TOSHIKI KOYAMA, KENJI HANABUSA, and HIROFUSA SHIRAI. "Catalytic Oxidation of 2-Mercaptoethanol by Cationic Water-soluble Phthalocyaninatocobalt(II) Complexes." Journal of Porphyrins and Phthalocyanines 01, no. 04 (1997): 309–13. http://dx.doi.org/10.1002/(sici)1099-1409(199710)1:4<309::aid-jpp36>3.0.co;2-r.

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The cationic amphiphilic cobalt(II) phthalocyanines have been prepared, and are characterized by UV-absorption spectra in water and organic solvents and in their mixtures. The monomer–dimer transformation equilibrium was affected by solvent polarity and the length of the alkyl chains in the amphiphilic parts. All complexes are efficient catalysts for the oxidation of 2-mercaptoethanol in the presence of dioxygen. The positive charge around the complex increases the acceleration of the dissociation of 2-mercaptoethanol under neutral pH. The catalytic activity is affected by the length of the alkyl chain; consequently the stability of aggregation between cobalt(II) phthalocyanines, as catalysts for the oxidation of 2-mercaptoethanol, can be changed.

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9

Pleska, Alexander, Iva Klichová, and Jindřich Pytela. "Grafting of Hydroxy-Terminated Polybutadiene with 2-Mercaptoethanol." Collection of Czechoslovak Chemical Communications 67, no. 12 (2002): 1899–910. http://dx.doi.org/10.1135/cccc20021899.

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A method for functionalization of hydroxy-terminated polybutadiene with 2-sulfanylethan-1-ol (2-mercaptoethanol) leading to a controlled increase in the OH functionality of the polymer was developed. The polybutadiene used contained about 65% of unsaturation in the form of 1,2-vinyl groups. The reaction was carried out without using volatile solvents. The radical addition of 2-sulfanylethan-1-ol on the polymer proceeded quantitatively and the products were free of the mercaptan odour. A series of functionalized polybutadienes were synthesized and characterized by their physical and chemical properties. The differential scanning calorimetry and thermogravimetric analysis measurements revealed that the functionalization improves the stability of the polybutadiene to thermooxidation. The obtained polymers were used for the preparation of polyurethane elastomers. The effect of the increased functionality of the polybutadiene on mechanical properties, thermal behavior and hydrolytic resistance of the elastomers was assessed.

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10

Miller, Benjamin J., Mivsam Yekutiel, A. Helena Sodergren, et al. "Overtone Spectra of 2-Mercaptoethanol and 1,2-Ethanedithiol." Journal of Physical Chemistry A 114, no. 48 (2010): 12692–700. http://dx.doi.org/10.1021/jp9112798.

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11

Goren, H. Joseph, and M. Fridkin. "THIOLYSIS OF Nim-2, 4-DINITROPHENYL-HISTIDINE PEPTIDES WITH 2-MERCAPTOETHANOL." International Journal of Peptide and Protein Research 11, no. 1 (2009): 1–8. http://dx.doi.org/10.1111/j.1399-3011.1978.tb02814.x.

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12

Gallagher, Clare N., та Reuben E. Huber. "Stabilities of uncomplemented and complemented M15 β-galactosidase (Escherichia coli) and the relationship to α-complementation". Biochemistry and Cell Biology 77, № 2 (1999): 109–18. http://dx.doi.org/10.1139/o99-025.

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M15 β-galactosidase (Escherichia coli) is a mutant form of β-galactosidase having residues 11-41 deleted. It is an inactive dimer but can be complemented to the active tetrameric form by the addition of a peptide containing the deleted residues. The activities of uncomplemented and complemented M15 β-galactosidases decreased starting at 42°C-uncomplemented over a narrow temperature range, complemented over a broad range. This is because uncomplemented protein is a simple dimer while complemented is a mix of interacting oligomers at high temperatures. The effects of added components on stability and α-complementation are best explained by binding effects on equilibria between native forms and forms susceptible to inactivation. Mg2+ stabilized complemented protein but destabilized uncomplemented protein (10× less Mg2+ was needed for complemented protein). α-Complementation increased somewhat at low Mg2+ but decreased at high Mg2+. These effects can be explained by differential Mg2+ binding to the native and susceptible forms. The enhancement of both stability and α-complementation by Na+ can be explained by preferential binding of Na+ to the native forms of both the uncomplemented and complemented proteins. Low 2-mercaptoethanol concentrations stabilized uncomplemented M15 β-galactosidase, but high concentrations destabilized it. All concentrations destabilized complemented M15 β-galactosidase. α-Complementation was enhanced by 2-mercaptoethanol. Thus, there is a correlation between stability of the uncomplemented protein and α-complementation at low 2-mercaptoethanol owing to interactions with native forms. The lack of correlation at higher 2-mercaptoethanol probably results from precipitation by 2-mercaptoethanol. In contrast to irreversible thermal inactivation, differences in reversible stability in urea were small. This suggests that quaternary structure and Mg2+ and Na+ sites are lost at low urea concentrations and are unimportant at the urea concentrations that result in reversible denaturation. Key words: β-galactosidase, α-complementation, stability.

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13

Duc, Michel, Bernard Boutevin, and Bruno Ameduri. "Unexpected Radical Telomerisation of Vinylidene Fluoride with 2-Mercaptoethanol." Molecules 26, no. 11 (2021): 3082. http://dx.doi.org/10.3390/molecules26113082.

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The radical telomerisation of vinylidene fluoride (VDF) with 2-mercaptoethanol as chain transfer agent (CTA) was studied to synthesise fluorinated telomers which bear a hydroxy end-group, such as H(VDF)nS(CH2)2OH, under thermal (di-tert-butyl peroxide as the initiator) or photochemical initiations. A careful structural study of a typical H-VDF-S(CH2)2OH telomer was performed by 1H and 19F NMR spectroscopy. These analytical methods allowed us to explore the selective addition of the thiyl radical onto the hydrogenated side of VDF, and the telomer containing one VDF unit was obtained selectively. Surprisingly, for higher [VDF]o initial concentrations, a monoadduct telomer was produced as well as PVDF homopolymer. This feature was related to the fast consumption of the CTA. The kinetics of radical telomerisation led to a quite high transfer constant of the CTA (40 at 140 °C) that evidences the formation of a monoadduct as the only telomer formed.

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14

Koshcheev, Borislav V., Roman A. Bredikhin, Alexander M. Maksimov, Vyacheslav E. Platonov, and Vladimir V. Shelkovnikov. "Transformations of perfluorotoluene by the action of 2-mercaptoethanol." Arkivoc 2019, no. 5 (2019): 89–95. http://dx.doi.org/10.24820/ark.5550190.p010.747.

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15

Casassas, E., C. Arino, M. Esteban, and C. Müller. "Anodic behaviour of 2-mercaptoethanol at a mercury electrode." Analytica Chimica Acta 206 (1988): 65–74. http://dx.doi.org/10.1016/s0003-2670(00)80831-4.

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16

Juszkiewicz, Adam, Mirosława Kot, and Wiesława Zaborska. "Calorimetric study of inhibition of urease by 2-mercaptoethanol." Thermochimica Acta 320, no. 1-2 (1998): 45–52. http://dx.doi.org/10.1016/s0040-6031(98)00397-9.

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17

Datskos, Panos G., and Isidor Sauers. "Detection of 2-mercaptoethanol using gold-coated micromachined cantilevers." Sensors and Actuators B: Chemical 61, no. 1-3 (1999): 75–82. http://dx.doi.org/10.1016/s0925-4005(99)00251-8.

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18

Georgoussi, Zafiroula, and Theodore G. Sotiroudis. "Inhibition of sarcoplasmic reticulum Ca2+-ATPase by 2-mercaptoethanol." Biochemical and Biophysical Research Communications 126, no. 3 (1985): 1196–200. http://dx.doi.org/10.1016/0006-291x(85)90312-2.

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19

Engelbrecht, F., G. Braun, V. Connor, M. Downham, J. A. Whitworth та D. W. Taylor. "Partial characterization of 2-β-mercaptoethanol-soluble surface-associated antigens of Onchocerca volvulus". Parasitology 102, № 3 (1991): 437–44. http://dx.doi.org/10.1017/s0031182000064416.

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Antigens were extracted from the epicuticle/cuticle of intact female Onchocerca volvulus using 2% 2-β-mercaptoethanol and 1% SDS. In Western blot analysis a human infection serum selected for its high antibody titre against whole worm homogenates did not recognize any component solubilized by 1% SDS. However, the same serum did bind at least 7 antigens among the material extracted with 2-β-mercaptoethanol. These antigens have apparent molecular weights (Mr) of: 15 000, 18 000, 28 000, 78 000, 98 000, 120 000 and 200 000. In ELISA using this preparation as target antigen, 151 out of 153 human infection sera gave positive results. An Onchocerca-specific IgG1 monoclonal antibody, designated Cam1, recognized the 28 000 Mr antigen, which is the most prominent antigen detected by Western blot analysis using human infection sera. In ELISA, using material affinity-purified with Cam1 as target antigen, 149 out of 153 human infection sera gave a positive IgG response. From a cDNA library three expressing clones were isolated with a rabbit serum raised against 2-β-mercaptoethanol solubilized material. One of these clones was recognized by the monoclonal antibody Cam1.

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20

Pacharra, Marlene, Michael Schäper, Stefan Kleinbeck, Meinolf Blaszkewicz, Oliver T. Wolf, and Christoph van Thriel. "Stress lowers the detection threshold for foul-smelling 2-mercaptoethanol." Stress 19, no. 1 (2015): 18–27. http://dx.doi.org/10.3109/10253890.2015.1105212.

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21

Asfari, M., D. Janjic, P. Meda, G. Li, P. A. Halban, and C. B. Wollheim. "Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines." Endocrinology 130, no. 1 (1992): 167–78. http://dx.doi.org/10.1210/endo.130.1.1370150.

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22

Reid, Nellone, and Robert Barat. "Kinetic Analysis of Hydroxide-Catalyzed, Aerobic Oxidation of 2-Mercaptoethanol." Chemical Engineering Communications 202, no. 9 (2014): 1231–34. http://dx.doi.org/10.1080/00986445.2014.907568.

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23

Mahmoud, Waleed E., Amal M. Al-Amri, and S. J. Yaghmour. "Low temperature synthesis of CdSe capped 2-mercaptoethanol quantum dots." Optical Materials 34, no. 7 (2012): 1082–86. http://dx.doi.org/10.1016/j.optmat.2012.01.001.

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24

Sonnet, Philip E., and Gordon G. Moore. "Thiol esters of 2-mercaptoethanol and 3-mercapto-1,2-propanediol." Lipids 24, no. 8 (1989): 743–45. http://dx.doi.org/10.1007/bf02535215.

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25

Mahmudov, Kamran T., Xudayar I. Hasanov, Abel M. Maharramov, et al. "A hexanuclear metallacrown palladium(II) cluster derived from 2-mercaptoethanol." Inorganic Chemistry Communications 29 (March 2013): 37–39. http://dx.doi.org/10.1016/j.inoche.2012.12.012.

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26

Muralidhara Rao, B., and A. Nityananda Shetty. "Complexometric determination of cadmium using 2-Mercaptoethanol as masking reagent." Mikrochimica Acta 130, no. 1-2 (1998): 103–4. http://dx.doi.org/10.1007/bf01254597.

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27

Al-Matar, Ali Kh, Bassam El-Eswed, and Maha F. Tutunji. "Kinetics of omeprazole degradation in the presence of 2-mercaptoethanol." International Journal of Chemical Kinetics 40, no. 6 (2008): 352–58. http://dx.doi.org/10.1002/kin.20307.

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28

Stiborová, Marie, and Sylva Leblová. "Effect of heterocyclic compounds and 2-mercaptoethanol on rape alcohol dehydrogenase." Collection of Czechoslovak Chemical Communications 52, no. 8 (1987): 2107–13. http://dx.doi.org/10.1135/cccc19872107.

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Rape alcohol dehydrogenase (ADH) is inhibited by heterocyclic compounds with a five- to six-membered ring (imidazole, pyrazole, 4-methylpyrazole, 3-methylpyrazole, pyridine, nicotine amide) and by o-phenanthroline, a heterocyclic, polycyclic compound. Pyrazole and its derivatives, imidazole and pyridine, are competitive inhibitors with respect to ethanol. Nicotine amide and o-phenanthroline behave as mixed inhibitors (competitive – noncompetitive) with respect to the substrate. The addition of Zn2+-ions to the reaction medium interferes with the competition by o-phenanthroline. 4-Methylpyrazole and pyrazole are the strongest inhibitors of rape ADH. 2-Mercaptoethanol is an inhibitor of rape ADH which competes with ethanol and the coenzyme. The reversible inhibition by mercaptoethanol changes into the irreversible inactivation of the enzyme. The binding of inhibitors to the zinc atom present in the molecule of rape ADH and the localization of the metal in the enzyme protein have been studied.

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29

Sugama, Kazushige, Yuziro Namba, Masakazu Hatanaka, and Masao Hanaoka. "2-Mercaptoethanol Acts as a Potentiating Factor of Interleukin-2-Dependent Lymphocyte Proliferation." Microbiology and Immunology 31, no. 7 (1987): 691–700. http://dx.doi.org/10.1111/j.1348-0421.1987.tb03130.x.

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30

Sugama, Kazushige, Yuziro Namba, Masakazu Hatanaka, and Masao Hanaoka. "2-Mercaptoethanol acts at the restricted stage of interleukin-2 dependent lymphocyte proliferation." Journal of Cellular Physiology 131, no. 1 (1987): 23–28. http://dx.doi.org/10.1002/jcp.1041310105.

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31

Gasanov, H., A. Azizova, and Sh. Kasumov. "SULFOBRIDGED POLYNUCLEAR COMPLEXES OF PLATINUM (II) AND PALLADIUM (II)." Sciences of Europe, no. 115 (April 24, 2023): 21–27. https://doi.org/10.5281/zenodo.7857908.

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When the platinum (II) and palladium (II) salts interact with ligands such as cystamine-(mercamine) HSCH<sub>2</sub>CH<sub>2</sub>NH<sub>2</sub> and 2-mercaptoethanol HSCH2CH2OH under certainconditions, polynuclear complexes of the compositions are obtained: [Pt<sub>6</sub>(SCH<sub>2</sub>CH<sub>2</sub>NH<sub>2</sub>)<sub>8</sub>]Cl<sub>4</sub>.5H<sub>2</sub>O and [Pd<sub>6</sub>(SCH<sub>2</sub>CH<sub>2</sub>OH)8]Cl<sub>4</sub>. In a comparative study of the IR and X-ray spectra of synthesized complexes and ligands, as well as the results of X-ray diffraction studies, it was established that sulfur atoms of 2-mercaptoethanol occupy a bridge positionwith mixed coordination of ligands in the palladium complex. In the platinum (II) complex bidentate coordination of ligands is realized through sulfur and nitrogen atoms.

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32

Liu, Feng, Ying Tang, Yongqing Kuang, et al. "An activatable fluorescent probe with an ultrafast response and large Stokes shift for live cell bioimaging of hypochlorous acid." RSC Advances 6, no. 109 (2016): 107910–15. http://dx.doi.org/10.1039/c6ra22686h.

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33

Nabiyouni, Gholamreza, Parviz Boroojerdian, Kambiz Hedayati, and Davood Ghanbari. "A Simple Method for Synthesis of PbS Nanoparticles Using 2-Mercaptoethanol as the Capping Agent." High Temperature Materials and Processes 31, no. 6 (2012): 723–25. http://dx.doi.org/10.1515/htmp-2011-0155.

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AbstractLead sulfide nanoparticles were synthesized at room temperature via a simple chemical reaction. In this synthesis, 2-mercaptoethanolwas used as the capping agent and sodium sulfide was used as a sulfur source. The products were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), ultraviolet-visible (UV-vis) spectroscopy and Fourier transform infrared (FT-IR) spectroscopy Electron microscopy study showed that without using a capping agent the bulk PbS is obtained, while adding the mercaptoethanol leads to production of nanoparticles. We found that the electronic absorption spectra as well as the particle sizes depend on the used capping agents. Two exitonic peaks with a large blue shift were observed when mercaptoethanol was used.

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34

Attia, Tamer Z., Mohamed Elnady, and Sayed M. Derayea. "A highly sensitive fluorimetric protocol based on isoindole formation for determination of gabapentin." RSC Advances 9, no. 51 (2019): 29942–48. http://dx.doi.org/10.1039/c9ra06164a.

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A new, simple, highly sensitive and selective spectrofluorimetric method was developed for determination of gabapentin through its derivatization with o-phthalaldehyde in the presence of 2-mercaptoethanol.

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35

Ren, Gang, Qiuyan Hao, Jing Mao, et al. "Ultrafast fabrication of nickel sulfide film on Ni foam for efficient overall water splitting." Nanoscale 10, no. 36 (2018): 17347–53. http://dx.doi.org/10.1039/c8nr05494k.

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36

Mateu-de-Antonio, E. M., M. Martin, and M. Soler. "Use of indirect enzyme-linked immunosorbent assay with hot saline solution extracts of a variant (M—) strain of Brucella canis for diagnosis of brucellosis in dogs." American Journal of Veterinary Research 54, no. 7 (1993): 1043–46. http://dx.doi.org/10.2460/ajvr.1993.54.07.1043.

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Summary An elisa, using hot saline solution extracts (hss) of a less-mucoid variant (M—) strain of Brucella canis as antigen, was developed for detection of antibodies against B canis in dogs. The test was applied to 177 field serum samples previously tested by use of the 2-mercaptoethanol rapid slide agglutination test, 2-mercaptoethanol-tube agglutination test, and agar gel immunodiffusion containing hss and cytoplasmic antigens of B canis. Results indicated that this elisa seems to be highly specific (95.6%) and slightly less sensitive (93.8%). The hss obtained from B canis wild-type RM 6/66 also have been used, but in our study, it seemed to be unsuitable for use in elisa because of the high background values observed for sera with negative test results.

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37

Ahmed. "Comparing of Routine 2 Mercaptoethanol (2ME) and Coombs Wright Plus 2ME." American Journal of Infectious Diseases 8, no. 1 (2012): 1–4. http://dx.doi.org/10.3844/ajidsp.2012.1.4.

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38

KATOW, Shigetaka, and Akira SUGIURA. "CONFORMATIONAL CHANGE OF RUBELLA VIRUS SPIKE PROTEINS INDUCED BY 2-MERCAPTOETHANOL." Japanese Journal of Medical Science and Biology 41, no. 3 (1988): 109–15. http://dx.doi.org/10.7883/yoken1952.41.109.

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39

NOSAKA, YOSHIO, HARUO SHIGENO, and TOMOMI IKEUCHI. "FORMATION STEPS OF CdS CLUSTERS IN AQUEOUS SOLUTION CONTAINING 2-MERCAPTOETHANOL." Surface Review and Letters 03, no. 01 (1996): 1209–13. http://dx.doi.org/10.1142/s0218625x96002187.

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Growing steps of CdS clusters in aqueous solution containing HOCH 2CH2 SH (2-mercaptoethanol, RSH) was investigated by means of stopped flow optical spectroscopy and proton NMR spectroscopy. When the amount of RS − was 4 times larger than Cd 2+ ions at alkaline pH, the growing cluster showed the change in the absorption peak via 242, 260, 280, 308, and 315 nm. Initial three peaks were assigned to [ Cd 1( SR )4]2−, [ S 1 Cd 4(SR)12]6−, and [ S 1 Cd 8( SR )16]2−, respectively. Among them, the cluster at 280 nm was very stable, comparable with the reported thiophenolate cluster [ S 1 Cd 8( SPh )16]2−. The relationship between the size and the absorption maximum for ultrasmall CdS particles reported was continuous to that obtained for the clusters in the present study.

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40

YANG, YI, HONG-RUI WANG, and HAI-MENG ZHOU. "Kinetics of inhibition of aminoacylase activity by dithiothreitol or 2-mercaptoethanol." International Journal of Peptide and Protein Research 48, no. 6 (2009): 532–38. http://dx.doi.org/10.1111/j.1399-3011.1996.tb00872.x.

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41

Nagasawa, Herbert T., Jonathan F. Cohen, and William B. Rathbun. "MIXED DISULFIDES OF L-CYSTEINE AND ITS DERIVATIVES WITH 2-MERCAPTOETHANOL." Organic Preparations and Procedures International 28, no. 2 (1996): 237–41. http://dx.doi.org/10.1080/00304949609356530.

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42

Harsing, Laszlo G., Hideo Nagashima, E. Sylvester Vizi, and Deryck Duncalf. "Electrochemical determination of histamine derivatized with o-phthalaldehyde and 2-mercaptoethanol." Journal of Chromatography B: Biomedical Sciences and Applications 383 (January 1986): 19–26. http://dx.doi.org/10.1016/s0378-4347(00)83436-2.

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43

Muralidhara Rao, B. "Indirect Complexometric Determination of Bismuth Using 2-Mercaptoethanol as Masking Agent." Microchimica Acta 132, no. 1 (1999): 101–3. http://dx.doi.org/10.1007/s006040050049.

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44

AXELSSON, JAN-AXEL, BENGT KÄLLÉN, OLLE NILSSON, and GLAES TROPÉ. "EFFECT OF 2-MERCAPTOETHANOL ON THE MIXED LEUKOCYTE REACTION IN MAN." Acta Pathologica Microbiologica Scandinavica Section C Immunology 84C, no. 5 (2009): 390–96. http://dx.doi.org/10.1111/j.1699-0463.1976.tb00046.x.

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45

Retamal, Bernardo A., Mariana E. Vaschetto, and JoséH Zagal. "Catalytic electro-oxidation of 2-mercaptoethanol using cobalt phthalocyanine + poly(2-chloroaniline) modified electrodes." Journal of Electroanalytical Chemistry 431, no. 1 (1997): 1–5. http://dx.doi.org/10.1016/s0022-0728(97)00087-9.

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46

Guan, Hui-Ping, Bing-Hao Luo, and Chang-Ming Hu. "Study of the nucleophilic reaction of 2-mercaptoethanol with 1-fluoroalkyl-2-iodo-alkenes." Journal of Fluorine Chemistry 85, no. 2 (1997): 141–45. http://dx.doi.org/10.1016/s0022-1139(97)00074-2.

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47

Knezevic, Marija, Lynne Katsikas, and Ivanka Popovic. "The synthesis and characterization of 2-mercaptoethyl methacrylate." Chemical Industry 59, no. 11-12 (2005): 321–23. http://dx.doi.org/10.2298/hemind0512321k.

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The synthesis of 2-mercaptoethyl methacrylate from methacrylic acid and 2-mercaptoethanol by etherification using acetyl chloride as catalyst was optimized. The purity of the obtained product was controlled by gas chromatography and its identity confirmed by H-NMR and FTIR spectroscopy. 2-Mercapto-ethyl methacrylate could find application as a chain transfer agent in radical polymerizations.

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48

Moreno de Pedri, Eliane Cristina, Catiane Dos Santos Braga, Carlos Alberto Da Cunha Oliveira, Auana Vicente Tiago, and Ana Aparecida Bandini Rossi. "AVALIAÇÃO DE DOIS MÉTODOS DE TRITURAÇÃO FOLIAR DE Acianthera ciliata (Orchidaceae) PARA EXTRAÇÃO DE DNA." Nativa 8, no. 1 (2020): 97. http://dx.doi.org/10.31413/nativa.v8i1.7252.

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Abstract:

O estabelecimento de protocolo de extração de DNA de espécies vegetais é uma técnica empregada para a obtenção de um DNA puro e de qualidade. Diante disso, objetivou-se neste estudo padronizar um protocolo para a extração de DNA da espécie Acianthera ciliata, visando posteriores estudos de diversidade genética. Foram testados dois métodos de trituração do tecido foliar, sendo eles: Tampão STE e nitrogênio líquido. Para cada método de trituração foram testadas duas concentrações de β-mercaptoetanol (0% e 2%). Os dois métodos utilizados, foram eficientes na extração do DNA genômico de A. ciliata. As amostras extraídas com 0% de β-mercaptoetanol, para os dois métodos, STE e nitrogênio líquido, apresentaram menor quantidade de DNA quando comparado com as amostras extraídas com 2% de β-mercaptoetanol. Os dois primers testados amplificaram regiões do genoma de A. ciliata. Para a extração de DNA de A. ciliata indica-se a utilização de CTAB 5% no tampão de extração e β-mercaptoetanol a 2%. Os iniciadores ISSR foram eficientes na amplificação e são recomendados para estudos de diversidade genética de A. ciliata.Palavras-chave: diversidade genética; CTAB; marcadores moleculares; orquídeas. EVALUATION OF TWO MACERATION METHODS IN Acianthera ciliata (Orchidaceae) LEAVES FOR DNA EXTRACTION ABSTRACT: The establishment of DNA extraction protocol for plant species is a technique employed to obtain pure and good quality DNA. In this study, we standardized a protocol for the extraction of DNA of the species Acianthera ciliata, aiming studies of genetic diversity subsequently. Two maceration methods for foliar tissue were tested, and they were STE buffer and liquid nitrogen. Two concentrations of β-mercaptoethanol (0% and 2%) were tested for each method. The two methods used were efficient for genomic DNA extraction of A. ciliata. In both methods the samples extracted using 0% of β-mercaptoethanol, they presented lesser amount of DNA than the samples extracted using 2% of β-mercaptoethanol. The two tested primers amplified genomic regions of A. ciliata. For the DNA extraction of A. ciliata, we indicated the use of CTAB 5% in the extraction buffer as well as β-mercaptoethanol to 2%. The ISSR primers were efficient in amplification and thus they are indicated for studies of genetic diversity of A. ciliata.Keywords: genetic diversity; CTAB; molecular markers; orchids.

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Falah, Iip Izul, Triyono Triyono, and Catur Atmaji. "DETERMINATION OF CONSECUTIVE REACTION RATE CONSTANTS BETWEEN GLYCINE AND ISOLEUCINE WITH o-PHTHALDIALDEHYDE AND 2-MERCAPTOETHANOL FROM SINGLE EXPERIMENT DATA." Indonesian Journal of Chemistry 11, no. 1 (2011): 21–25. http://dx.doi.org/10.22146/ijc.21414.

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Kinetics study of chemical reaction is important in producing optimum product, designing a reactor, analyzing a compound, and many other purposes. This work presents the result of study of consecutive pseudo first order reaction between glycine and isoleucine with o-phthaldialdehyde and 2-mercaptoethanol in borate buffer solution at pH=9.0. Continuous detection was used to follow the change of fluorescence intensity of formed isoindole during the reaction. Although concentrations of initial compound and its derivative were not known; by using iteration method, the reaction constants could be determined with high precision from single experiment data. Result of the work showed that reaction rate constants k1 and k2 for consecutive reaction of glycine with excess of o-phthaldialdehyde and 2-mercaptoethanol were 18.75x10-3 and 26.70x10-5 s-1, respectively; whereas for isoleucine were 6.06x10-3 and 12.59x10-5 s-1, respectively.

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Streinz, Ludvík, Bohumír Koutek, and David Šaman. "An Effective Synthesis of 1,3-Oxathiolanes." Collection of Czechoslovak Chemical Communications 62, no. 4 (1997): 665–71. http://dx.doi.org/10.1135/cccc19970665.

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2-Alkyl- or 2,2-dialkyl-1,3-oxathiolanes can be effectively prepared from aldehydes or ketones and 2-mercaptoethanol, with triisopropylsilyl triflate as a catalyst. The reaction is over within minutes and, despite the fact that water is nor removed during the reaction, the yields of products are high.

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