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Journal articles on the topic '2D-DIGE ( Two Dimensional Differential In Gel Electrophoresis)'

Author: Grafiati
Published: 10 March 2023
Last updated: 31 July 2025

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1

Ercan, Huriye, Ulrike Resch, Felicia Hsu, et al. "A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection." Cells 12, no. 5 (2023): 747. http://dx.doi.org/10.3390/cells12050747.

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Proteomics is an indispensable analytical technique to study the dynamic functioning of biological systems via different proteins and their proteoforms. In recent years, bottom-up shotgun has become more popular than gel-based top-down proteomics. The current study examined the qualitative and quantitative performance of these two fundamentally different methodologies by the parallel measurement of six technical and three biological replicates of the human prostate carcinoma cell line DU145 using its two most common standard techniques, label-free shotgun and two-dimensional differential gel e
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Oliva, Karen, Gillian Barker, Clyde Riley, et al. "The effect of pre-existing maternal obesity on the placental proteome: two-dimensional difference gel electrophoresis coupled with mass spectrometry." Journal of Molecular Endocrinology 48, no. 2 (2012): 139–49. http://dx.doi.org/10.1530/jme-11-0123.

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Our aim was to study the protein expression profiles of placenta obtained from lean and obese pregnant women with normal glucose tolerance at the time of term Caesarean section. We used two-dimensional difference gel electrophoresis (2D-DIGE), utilising narrow-range immobilised pH gradient strips that encompassed the broad pH range of 4–5 and 5–6, followed by MALDI-TOF mass spectrometry of selected protein spots. Western blot and quantitative RT-PCR (qRT-PCR) analyses were performed to validate representative findings from the 2D-DIGE analysis. Eight proteins were altered (six down-regulated a
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Tajima, Takashi, Fusako Kito, Akihiko Yoshida, Akira Kawai, and Tadashi Kondo. "Calreticulin as A Novel Potential Metastasis-Associated Protein in Myxoid Liposarcoma, as Revealed by Two-Dimensional Difference Gel Electrophoresis." Proteomes 7, no. 2 (2019): 13. http://dx.doi.org/10.3390/proteomes7020013.

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Myxoid liposarcoma (MLS) is a mesenchymal malignancy. To identify innovate seeds for clinical applications, we examined the proteomes of primary tumor tissues from 10 patients with MLS with different statuses of postoperative metastasis. The protein expression profiles of tumor tissues were created, and proteins with differential expression associated with postoperative metastasis were identified by two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry. The validation was performed using specific antibodies and in vitro analyses. Using 2D-DIGE, we observed 1726 protein
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Dhingra, V., Q. Li, A. B. Allison, D. E. Stallknecht, and Z. F. Fu. "Proteomic Profiling and Neurodegeneration in West-Nile-Virus-Infected Neurons." Journal of Biomedicine and Biotechnology 2005, no. 3 (2005): 271–79. http://dx.doi.org/10.1155/jbb.2005.271.

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West Nile virus, a mosquito-borne flavivirus, is a human, equine, and avian pathogen. High-resolution two-dimensional differential-gel electrophoresis (2D-DIGE) was used to characterize protein expression in primary rat neurons and to examine the proteomic profiling to understand the pathogenesis of West-Nile-associated meningoencephalitis. Three pH ranges, 3–10, 4–7, and 5–6, were used to analyze the protein spots. The proteins are labeled with fluorescent dyes Cy3 and Cy5 before being separated on the basis of charge and size respectively on a two-dimensional platform. About 55 proteins show
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Di Carli, Mariasole, Anita Zamboni, Mario Enrico Pè, et al. "Two-Dimensional Differential in Gel Electrophoresis (2D-DIGE) Analysis of Grape Berry Proteome during Postharvest Withering." Journal of Proteome Research 10, no. 2 (2011): 429–46. http://dx.doi.org/10.1021/pr1005313.

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Wang, X., A. Kaya, and E. Memili. "195 SPERMATOZOAL PROTEIN MARKERS FOR ANGUS BULL FERTILITY." Reproduction, Fertility and Development 23, no. 1 (2011): 198. http://dx.doi.org/10.1071/rdv23n1ab195.

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Fertility is one of the most economically important traits controlling animal reproduction. Despite its significant economic impact, there are no reliable markers to predict semen quality. The objective of this study was to identify spermatozoal proteins associated with bull fertility, the ability of the sperm to fertilize the oocyte and support embryonic development. To accomplish our objectives, we isolated total spermatozoal proteins from 4 Angus bulls with different fertility phenotypes. Next, differentially expressed proteins were determined using two-dimensional differential in-gel elect
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Orenes-Piñero, Esteban, Marta Cortón, Pilar González-Peramato, et al. "Searching Urinary Tumor Markers for Bladder Cancer Using a Two-Dimensional Differential Gel Electrophoresis (2D-DIGE) Approach." Journal of Proteome Research 6, no. 11 (2007): 4440–48. http://dx.doi.org/10.1021/pr070368w.

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Subong, Bryan John J., Arturo O. Lluisma, Rhodora V. Azanza, and Lilibeth A. Salvador-Reyes. "Differentiating Two Closely Related Alexandrium Species Using Comparative Quantitative Proteomics." Toxins 13, no. 1 (2020): 7. http://dx.doi.org/10.3390/toxins13010007.

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Alexandrium minutum and Alexandrium tamutum are two closely related harmful algal bloom (HAB)-causing species with different toxicity. Using isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics and two-dimensional differential gel electrophoresis (2D-DIGE), a comprehensive characterization of the proteomes of A. minutum and A. tamutum was performed to identify the cellular and molecular underpinnings for the dissimilarity between these two species. A total of 1436 proteins and 420 protein spots were identified using iTRAQ-based proteomics and 2D-DIGE, resp
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Martínez-Gomariz, M., M. L. HernÁez, D. GutiÉrrez, P. XimÉnez-EmbÚn, and G. PrÉstamo. "Proteomic Analysis by Two-Dimensional Differential Gel Electrophoresis (2D DIGE) of a High-Pressure Effect in Bacillus cereus." Journal of Agricultural and Food Chemistry 57, no. 9 (2009): 3543–49. http://dx.doi.org/10.1021/jf803272a.

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10

Gemoll, Timo, Svitlana Rozanova, Christian Röder, et al. "Protein Profiling of Serum Extracellular Vesicles Reveals Qualitative and Quantitative Differences after Differential Ultracentrifugation and ExoQuick™ Isolation." Journal of Clinical Medicine 9, no. 5 (2020): 1429. http://dx.doi.org/10.3390/jcm9051429.

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Solid tumor biopsies are the current standard for precision medicine. However, the procedure is invasive and not always feasible. In contrast, liquid biopsies, such as serum enriched for extracellular vesicles (EVs) represent a non-invasive source of cancer biomarkers. In this study, we compared two EV isolation methods in the context of the protein biomarker detection in inflammatory bowel disease (IBD) and colorectal cancer (CRC). Using serum samples of a healthy cohort as well as CRC and IBD patients, EVs were isolated by ultracentrifugation and ExoQuick™ in parallel. EV associated protein
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Huang, Cheng-Yi, Ko-Chao Lee, Shui-Yi Tung, et al. "2D-DIGE-MS Proteomics Approaches for Identification of Gelsolin and Peroxiredoxin 4 with Lymph Node Metastasis in Colorectal Cancer." Cancers 14, no. 13 (2022): 3189. http://dx.doi.org/10.3390/cancers14133189.

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Background/Aims: A combination of fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry approach was used to search for potential markers for prognosis and intervention of colorectal cancer (CRC) at different stages of lymph node metastasis (LMN). This quantitative proteomic survey aimed to investigate the LNM-associated proteins and evaluate the clinicopathological characteristics of these target proteins in CRC from stage I to stage IV. Methods: Sixteen CRC cases were categorized into paired non-
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Repetto, Ombretta, Valli De Re, Paolo Giuffrida, et al. "Proteomics signature of autoimmune atrophic gastritis: towards a link with gastric cancer." Gastric Cancer 24, no. 3 (2021): 666–79. http://dx.doi.org/10.1007/s10120-020-01148-3.

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Abstract Background Autoimmune atrophic gastritis (AAG) is a chronic disease that can progress to gastric cancer (GC). To better understand AAG pathology, this proteomics study investigated gastric proteins whose expression levels are altered in this disease and also in GC. Methods Using two-dimensional difference gel electrophoresis (2D-DIGE), we compared protein maps of gastric corpus biopsies from AAG patients and controls. Differentially abundant spots (|fold change|≥ 1.5, P < 0.01) were selected and identified by LC–MS/MS. The spots were further assessed in gastric antrum biopsies from
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Ferreira, Tamara Aparecida Reis, Hélida Monteiro de Andrade, Paulo Madureira de Pádua, et al. "Identification of potential biomarkers for systemic lupus erythematosus diagnosis using two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry." Autoimmunity 50, no. 4 (2017): 247–56. http://dx.doi.org/10.1080/08916934.2017.1344975.

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Sabha, Bassam H., Afshan Masood, Ibrahim O. Alanazi, et al. "Comparative Analysis of Milk Fat Globular Membrane (MFGM) Proteome between Saudi Arabia Camelus dromedary Safra and Wadha Breeds." Molecules 25, no. 9 (2020): 2146. http://dx.doi.org/10.3390/molecules25092146.

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Camel milk is traditionally known to have medicinal properties and many potential health benefits. Natural milk contains many soluble proteins and nanoparticles, such as a milk fat globule membrane (MFGM), a three-layered membrane covering of milk fat globule mainly composed of proteins and lipids, which plays an important role in human health. MFGM proteins account for 1%–4% of total milk proteins, and their nutritive value and distribution depends on the different breeds. The differential composition of these membrane proteins among different camel breeds has not been explored. The current s
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Zheng, Dawei, Limin Xu, Lebo Sun, et al. "Comparison of the Ventricle Muscle Proteome between Patients with Rheumatic Heart Disease and Controls with Mitral Valve Prolapse: HSP 60 May Be a Specific Protein in RHD." BioMed Research International 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/151726.

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Objective. Rheumatic heart disease (RHD) is a serious autoimmune heart disease. The present study was aimed at identifying the differentially expressed proteins between patients with RHD and controls with mitral valve prolapse.Methods. Nine patients with RHD and nine controls with mitral valve prolapsed were enrolled for this study. Two-dimensional difference in-gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were performed.Results. A total of 39 protein spots with differential expressions were identified between the
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Visker, Joseph R., Lawrence J. Dangott, Eric C. Leszczynski, and David P. Ferguson. "Postnatal Growth Restriction in Mice Alters Cardiac Protein Composition and Leads to Functional Impairment in Adulthood." International Journal of Molecular Sciences 21, no. 24 (2020): 9459. http://dx.doi.org/10.3390/ijms21249459.

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Postnatal growth restriction (PGR) increases the risk for cardiovascular disease (CVD) in adulthood, yet there is minimal mechanistic rationale for the observed pathology. The purpose of this study was to identify proteomic differences in hearts of growth-restricted and unrestricted mice, and propose mechanisms related to impairment in adulthood. Friend leukemia virus B (FVB) mouse dams were fed a control (CON: 20% protein), or low-protein (LP: 8% protein) isocaloric diet 2 weeks before mating. LP dams produce 20% less milk, inducing growth restriction. At birth (postnatal; PN1), pups born to
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Jedmowski, Christoph, Ahmed Ashoub, Tobias Beckhaus, Thomas Berberich, Michael Karas, and Wolfgang Brüggemann. "Comparative Analysis of Sorghum bicolor Proteome in Response to Drought Stress and following Recovery." International Journal of Proteomics 2014 (October 1, 2014): 1–10. http://dx.doi.org/10.1155/2014/395905.

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The adaptive response of Sorghum bicolor landraces from Egypt to drought stress and following recovery was analyzed using two-dimensional difference gel electrophoresis, 2D-DIGE. Physiological measurements and proteome alterations of accession number 11434, drought tolerant, and accession number 11431, drought sensitive, were compared to their relative control values after drought stress and following recovery. Differentially expressed proteins were analysed by Matrix assisted laser desorption ionisation time-of-flight mass spectrometry, MALDI-TOF-MS. Alterations in protein contents related to
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Castillejo, Ma Ángeles, Mónica Fernández-Aparicio, and Diego Rubiales. "Proteomic analysis by two-dimensional differential in gel electrophoresis (2D DIGE) of the early response of Pisum sativum to Orobanche crenata." Journal of Experimental Botany 63, no. 1 (2011): 107–19. http://dx.doi.org/10.1093/jxb/err246.

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Li, Fei, Ding-nan Chen, Cheng-wu He, et al. "Identification of urinary Gc-globulin as a novel biomarker for bladder cancer by two-dimensional fluorescent differential gel electrophoresis (2D-DIGE)." Journal of Proteomics 77 (December 2012): 225–36. http://dx.doi.org/10.1016/j.jprot.2012.09.002.

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Abdelmegid, Shaimaa, David Kelton, Jeff Caswell, and Gordon Kirby. "Proteomic 2D-DIGE Analysis of Milk Whey from Dairy Cows with Staphylococcus aureus Mastitis Reveals Overexpression of Host Defense Proteins." Microorganisms 8, no. 12 (2020): 1883. http://dx.doi.org/10.3390/microorganisms8121883.

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Bovine mastitis remains a primary focus of dairy cattle disease research due to its considerable negative economic impact on the dairy industry. Subclinical mastitis (SCM), commonly caused by Staphylococcus aureus, lacks overt clinical signs and the diagnosis is based on bacteriological culture and somatic cell counts of milk, both of which have limitations. The main objective of this study was to identify, characterize and quantify the differential abundance of milk whey proteins from cows with S. aureus SCM compared to whey from healthy cows. Using two-dimensional differential gel electropho
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Chen, Yi-Wen, Hsiu-Chuan Chou, Szu-Ting Lin, et al. "Cardioprotective Effects of Quercetin in Cardiomyocyte under Ischemia/Reperfusion Injury." Evidence-Based Complementary and Alternative Medicine 2013 (2013): 1–16. http://dx.doi.org/10.1155/2013/364519.

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Quercetin, a polyphenolic compound existing in many vegetables, fruits, has antiinflammatory, antiproliferation, and antioxidant effect on mammalian cells. Quercetin was evaluated for protecting cardiomyocytes from ischemia/reperfusion injury, but its protective mechanism remains unclear in the current study. The cardioprotective effects of quercetin are achieved by reducing the activity of Src kinase, signal transducer and activator of transcription 3 (STAT3), caspase 9, Bax, intracellular reactive oxygen species production, and inflammatory factor and inducible MnSOD expression. Fluorescence
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Hu, Jian Da, Min-Hui Lin, Xin-Ji Chen, Ting-Bo Liu, and Lian-Huang Lv. "Differential Protein Expressions Between Leukemia HL-60 and Adriamycin-Resistant HL-60 Cell Lines by Comparative Preteomic Analysis." Blood 112, no. 11 (2008): 5052. http://dx.doi.org/10.1182/blood.v112.11.5052.5052.

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Abstract Resistance is a major problem of chemotherapy failure in acute leukemia. Although multi-drug resistance is an important factor, the exact mechanisms of resistance remain to be clarified. With the aim of better understanding the protein involvement in development of resistance mechanisms, a comparative proteomic analysis——two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) was used to compare differential protein expression profiles in adriamycin-resistant acute myeloid leukemia (AML) HL-60/ADR and sensitive HL-60 cell line. Total cellular proteins extracted from HL-
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Cazenave, Jean-Pierre, Philippe Ohlmann, Hervé Isola, and Christian Gachet. "Photochemical Pathogen Inactivation Treatment of Human Plasma (amotosalen + UVA) Has No Major Impact on the Protein expression pattern Assessed by a 2-DIGE Proteomic Assay." Blood 112, no. 11 (2008): 1994. http://dx.doi.org/10.1182/blood.v112.11.1994.1994.

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Abstract Background: Photochemical treatment (PCT) using amotosalen HCl and UVA light (3 J/cm2: 320 – 400 nm) inactivates pathogens and leukocytes in therapeutic single donor apheresis plasma prepared within 8 hr of collection (INTERCEPTTM [I-FFP], Cerus Europe, Amersfoort, Netherlands). Clinical trials demonstrated efficacy and safety supporting a Class III CE mark and AFSSAPS (Medicinal Products Agency of France) registration of I-FFP for primary therapeutic indications including: congenital and acquired coagulopathies and TTP. Although no evidence of immune response to neo-antigens has been
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Capaci, Valeria, Giorgio Arrigoni, Lorenzo Monasta, et al. "Phospho-DIGE Identified Phosphoproteins Involved in Pathways Related to Tumour Growth in Endometrial Cancer." International Journal of Molecular Sciences 24, no. 15 (2023): 11987. http://dx.doi.org/10.3390/ijms241511987.

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Endometrial cancer (EC) is the most common gynecologic malignancy of the endometrium. This study focuses on EC and normal endometrium phosphoproteome to identify differentially phosphorylated proteins involved in tumorigenic signalling pathways which induce cancer growth. We obtained tissue samples from 8 types I EC at tumour stage 1 and 8 normal endometria. We analyzed the phosphoproteome by two-dimensional differential gel electrophoresis (2D-DIGE), combined with immobilized metal affinity chromatography (IMAC) and mass spectrometry for protein and phosphopeptide identification. Quantities o
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Johnson, D. Thor, Robert A. Harris, Stephanie French, Angel Aponte, and Robert S. Balaban. "Proteomic changes associated with diabetes in the BB-DP rat." American Journal of Physiology-Endocrinology and Metabolism 296, no. 3 (2009): E422—E432. http://dx.doi.org/10.1152/ajpendo.90352.2008.

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These studies were structured with the aim of utilizing emerging technologies in two-dimensional (2D) gel electrophoresis and mass spectrometry to evaluate protein expression changes associated with type 1 diabetes. We reasoned that a broad examination of diabetic tissues at the protein level might open up novel avenues of investigation of the metabolic and signaling pathways that are adversely affected in type 1 diabetes. This study compared the protein expression of the liver, heart, and skeletal muscle of diabetes-prone rats and matched control rats by semiquantitative liquid chromatography
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Benabdelkamel, Hicham, Afshan Masood, Meshail Okla, Mohammed Y. Al-Naami, and Assim A. Alfadda. "A Proteomics-Based Approach Reveals Differential Regulation of Urine Proteins between Metabolically Healthy and Unhealthy Obese Patients." International Journal of Molecular Sciences 20, no. 19 (2019): 4905. http://dx.doi.org/10.3390/ijms20194905.

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Metabolic dysfunction associated with obesity threatens to inundate health care resources by increasing the incidences of obesity-related diseases. The aim of the present study was to investigate the changes in the urinary proteome of 18 individuals classified into metabolically healthy obese (MHO) and metabolically unhealthy obese (MUHO) patients. Proteome analysis was performed using the two-dimensional difference in gel electrophoresis (2D-DIGE) coupled with mass spectrometry (MS). Upon analysis, a total of 54 proteins were found to be affected with ≥1.5-fold change (ANOVA, p ≤ 0.05), of wh
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Capitanio, Daniele, Pietro Barbacini, Beatrice Arosio, et al. "Can Serum Nitrosoproteome Predict Longevity of Aged Women?" International Journal of Molecular Sciences 21, no. 23 (2020): 9009. http://dx.doi.org/10.3390/ijms21239009.

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Aging is characterized by increase in reactive oxygen (ROS) and nitrogen (RNS) species, key factors of cardiac failure and disuse-induced muscle atrophy. This study focused on serum nitroproteome as a trait of longevity by adopting two complementary gel-based techniques: two-dimensional differential in gel electrophoresis (2-D DIGE) and Nitro-DIGE coupled with mass spectrometry of albumin-depleted serum of aged (A, n = 15) and centenarian (C, n = 15) versus young females (Y, n = 15). Results indicate spots differently expressed in A and C compared to Y and spots changed in A vs. C. Nitro-DIGE
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Buttacavoli, Miriam, Nadia Ninfa Albanese, Elena Roz, Ida Pucci-Minafra, Salvatore Feo, and Patrizia Cancemi. "Proteomic Profiling of Colon Cancer Tissues: Discovery of New Candidate Biomarkers." International Journal of Molecular Sciences 21, no. 9 (2020): 3096. http://dx.doi.org/10.3390/ijms21093096.

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Colon cancer is an aggressive tumor form with a poor prognosis. This study reports a comparative proteomic analysis performed by using two-dimensional differential in-gel electrophoresis (2D-DIGE) between 26 pooled colon cancer surgical tissues and adjacent non-tumoral tissues, to identify potential target proteins correlated with carcinogenesis. The DAVID functional classification tool revealed that most of the differentially regulated proteins, acting both intracellularly and extracellularly, concur across multiple cancer steps. The identified protein classes include proteins involved in cel
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Merkley, Mark A., Paul M. Weinberger, Lana L. Jackson, Robert H. Podolsky, Jeffrey R. Lee, and William S. Dynan. "2D-DIGE proteomic characterization of head and neck squamous cell carcinoma." Otolaryngology–Head and Neck Surgery 141, no. 5 (2009): 626–32. http://dx.doi.org/10.1016/j.otohns.2009.08.011.

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Objective: Identify proteins that are differentially expressed between head and neck squamous cell cancer (HNSCC) and patient-matched normal adjacent tissue, and validate findings in a separate patient cohort. Study Design: Cross-sectional study of surgical specimens. Setting: Tertiary care academic medical center. Subjects and Methods: Laser capture microdissection and two-dimensional difference gel electrophoresis were used previously to establish proteomic profiles for tumor and normal adjacent tissue from 14 patients. Here, significance analysis of microarray was used to rank candidate bio
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Corzett, Todd H., Imola K. Fodor, Megan W. Choi, et al. "Statistical Analysis of Variation in the Human Plasma Proteome." Journal of Biomedicine and Biotechnology 2010 (2010): 1–12. http://dx.doi.org/10.1155/2010/258494.

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Quantifying the variation in the human plasma proteome is an essential prerequisite for disease-specific biomarker detection. We report here on the longitudinal and individual variation in human plasma characterized by two-dimensional difference gel electrophoresis (2-D DIGE) using plasma samples from eleven healthy subjects collected three times over a two week period. Fixed-effects modeling was used to remove dye and gel variability. Mixed-effects modeling was then used to quantitate the sources of proteomic variation. The subject-to-subject variation represented the largest variance compone
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Repetto, Ombretta, Federica Lovisa, Caterina Elia, et al. "Proteomic Exploration of Plasma Exosomes and Other Small Extracellular Vesicles in Pediatric Hodgkin Lymphoma: A Potential Source of Biomarkers for Relapse Occurrence." Diagnostics 11, no. 6 (2021): 917. http://dx.doi.org/10.3390/diagnostics11060917.

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Exosomes and other small extracellular vesicles (EVs) are potential sources of cancer biomarkers. Plasma-derived EVs have not yet been studied in pediatric Hodgkin lymphoma (HL), for which predictive biomarkers of relapse are greatly needed. In this two-part proteomic study, we used two-dimensional difference gel electrophoresis (2D-DIGE) followed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) to analyze EV proteins of plasma collected at diagnosis from children with nodular sclerosis HL, relapsed or not. EVs isolated using membrane affinity had radii ranging from 20 to 130 nm an
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van Zanten, Gabriella C., Nadja Sparding, Avishek Majumder, Sampo J. Lahtinen, Birte Svensson та Susanne Jacobsen. "The Differential Proteome of the ProbioticLactobacillus acidophilusNCFM Grown on the Potential Prebiotic Cellobiose Shows Upregulation of Twoβ-Glycoside Hydrolases". BioMed Research International 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/347216.

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Probiotics, prebiotics, and combinations thereof, that is, synbiotics, are known to exert beneficial health effects in humans; however interactions between pro- and prebiotics remain poorly understood at the molecular level. The present study describes changes in abundance of different proteins of the probiotic bacteriumLactobacillus acidophilusNCFM (NCFM) when grown on the potential prebiotic cellobiose as compared to glucose. Cytosolic cell extract proteomes after harvest at late exponential phase of NCFM grown on cellobiose or glucose were analyzed by two dimensional difference gel electrop
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Kratochwill, Klaus, Thorsten O. Bender, Anton M. Lichtenauer, et al. "Cross-Omics Comparison of Stress Responses in Mesothelial Cells Exposed to Heat- versus Filter-Sterilized Peritoneal Dialysis Fluids." BioMed Research International 2015 (2015): 1–12. http://dx.doi.org/10.1155/2015/628158.

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Recent research suggests that cytoprotective responses, such as expression of heat-shock proteins, might be inadequately induced in mesothelial cells by heat-sterilized peritoneal dialysis (PD) fluids. This study compares transcriptome data and multiple protein expression profiles for providing new insight into regulatory mechanisms. Two-dimensional difference gel electrophoresis (2D-DIGE) based proteomics and topic defined gene expression microarray-based transcriptomics techniques were used to evaluate stress responses in human omental peritoneal mesothelial cells in response to heat- or fil
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Li, Shenjie, Wei Xiang, Junjie Tian, et al. "Bone Marrow-Derived Mesenchymal Stem Cells Differentially Affect Glioblastoma Cell Proliferation, Migration, and Invasion: A 2D-DIGE Proteomic Analysis." BioMed Research International 2021 (February 11, 2021): 1–13. http://dx.doi.org/10.1155/2021/4952876.

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Bone marrow-derived mesenchymal stem cells (BM-MSCs) display high tumor tropism and cause indirect effects through the cytokines they secrete. However, the effects of BM-MSCs on the biological behaviors of glioblastoma multiforme remain unclear. In this study, the conditioned medium from BM-MSCs significantly inhibited the proliferation of C6 cells ( P < 0.05 ) but promoted their migration and invasion ( P < 0.05 ). Two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) proteomic analysis revealed 17 proteins differentially expressed in C6 cells exposed to the BM-MSC-condi
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Gao, Liyan, Aili Wang, Xiaohui Li, et al. "Wheat quality related differential expressions of albumins and globulins revealed by two-dimensional difference gel electrophoresis (2-D DIGE)." Journal of Proteomics 73, no. 2 (2009): 279–96. http://dx.doi.org/10.1016/j.jprot.2009.09.014.

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Brechlin, Peter, Olaf Jahn, Petra Steinacker, et al. "Cerebrospinal fluid-optimized two-dimensional difference gel electrophoresis (2-D DIGE) facilitates the differential diagnosis of Creutzfeldt-Jakob disease." PROTEOMICS 8, no. 20 (2008): 4357–66. http://dx.doi.org/10.1002/pmic.200800375.

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Wang, Ruomei, Jisu Wu, Xiong Deng, Dongmiao Liu, and Yueming Yan. "Drought-responsive protein identification in developing grains of a wheat–Haynaldia villosa 6VS/6AL translocation line." Crop and Pasture Science 69, no. 12 (2018): 1182. http://dx.doi.org/10.1071/cp18303.

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Drought is a widespread abiotic stress that has a detrimental effect on both yield and quality of wheat. Discovery and utilisation of drought-resistant gene resources from wheat-related species may help to mitigate effects of drought and decrease yield loss. In this study, we used a comparative proteome approach to identify potential drought-resistance proteins from a wheat (Triticum aestivum L.)–Haynaldia villosa (L.) Schur 6VS/6AL translocation line. Drought experiments showed that introgression of the H. villosa 6VS chromosome short arm into common wheat cultivar Yangmai 5 through 6VS/6AL t
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Manivannan, Bhagyashree, Pisana Rawson, T. William Jordan, et al. "Identification of Cytokeratin 18 as a Biomarker of Mouse and Human Hepatosplenic Schistosomiasis." Infection and Immunity 79, no. 5 (2011): 2051–58. http://dx.doi.org/10.1128/iai.01214-10.

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ABSTRACTPreviously, we demonstrated unique protein expression patterns in 20-week-Schistosoma mansoni-infected CBA/J mice with moderate splenomegaly syndrome (MSS) or hypersplemomegaly syndrome (HSS). To better understand the development of severe pathology, we compared the two-dimensional differential in-gel electrophoresis (2D-DIGE) proteomic signatures of livers from uninfected mice and mice infected for 6, 8, 12, or 20 weeks and found significant changes in collagen isoforms, interleukin-2 (IL-2), cytokeratin 18, hydroxyproline,S. mansoniphosphoenolpyruvate carboxykinase, major urinary pro
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Fuhrmann, Dominik C., Michaela Tausendschön, Ilka Wittig, et al. "Inactivation of Tristetraprolin in Chronic Hypoxia Provokes the Expression of Cathepsin B." Molecular and Cellular Biology 35, no. 3 (2014): 619–30. http://dx.doi.org/10.1128/mcb.01034-14.

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Macrophages play important roles in many diseases and are frequently found in hypoxic areas. A chronic hypoxic microenvironment alters global cellular protein expression, but molecular details remain poorly understood. Although hypoxia-inducible factor (HIF) is an established transcription factor allowing adaption to acute hypoxia, responses to chronic hypoxia are more complex. Based on a two-dimensional differential gel electrophoresis (2D-DIGE) approach, we aimed to identify proteins that are exclusively expressed under chronic but not acute hypoxia (1% O2). One of the identified proteins wa
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Takeda, K., M. Tasai, S. Akagi, et al. "62 COMPARATIVE PROTEOMIC ANALYSIS OF LIVER MITOCHONDRIA DERIVED FROM DECEASED NEWBORN CLONED CALVES AND ADULT CLONES BY TWO-DIMENSIONAL DIFFERENTIAL GEL ELECTROPHORESIS." Reproduction, Fertility and Development 23, no. 1 (2011): 137. http://dx.doi.org/10.1071/rdv23n1ab62.

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Aberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. The inability to establish functional interactions between donor nucleus and recipient mitochondria is also likely responsible for developmental deficiency. However, an understanding of the expressed proteins in cattle is lacking. In the present study, alterations in mitochondrial protein levels between somatic cell nuclear transferred (SCNT) and control animals (mostly produced by AI) were investigated. Nuclear transfer was performed using donor cells prepared from cumulus cells (B1), ear
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Masood, Afshan, Hicham Benabdelkamel, Assim A. Alfadda, et al. "Proteomic Profiling Reveals Novel Molecular Insights into Dysregulated Proteins in Established Cases of Rheumatoid Arthritis." Proteomes 13, no. 3 (2025): 32. https://doi.org/10.3390/proteomes13030032.

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Background: Rheumatoid arthritis (RA) is a chronic autoimmune disorder that predominantly affects synovial joints, leading to inflammation, pain, and progressive joint damage. Despite therapeutic advancements, the molecular basis of established RA remains poorly defined. Methods: In this study, we conducted an untargeted plasma proteomic analysis using two-dimensional differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in samples from RA patients and healthy controls in the discovery phase. Results: Signific
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42

Rosso, Marina, Lara Lapyckyj, María José Besso, et al. "Characterization of the molecular changes associated with the overexpression of a novel epithelial cadherin splice variant mRNA in a breast cancer model using proteomics and bioinformatics approaches: identification of changes in cell metabolism and an increased expression of lactate dehydrogenase B." Cancer & Metabolism 7, no. 1 (2019): 5. https://doi.org/10.1186/s40170-019-0196-9.

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<strong>Background: </strong>Breast cancer (BC) is the most common female cancer and the leading cause of cancer death in women worldwide. Alterations in epithelial cadherin (E-cadherin) expression and functions are associated to BC, but the underlying molecular mechanisms have not been fully elucidated. We have previously reported a novel human E-cadherin splice variant (E-cadherin variant) mRNA. Stable transfectants in MCF-7 human BC cells (MCF7Ecadvar) depicted fibroblast-like cell morphology, E-cadherin wild-type downregulation, and other molecular changes characteristic of the epithelial-
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Martínez-Aguilar, Mayeli M., Diana I. Aparicio-Bautista, Eric G. Ramírez-Salazar, et al. "Serum Proteomic Analysis Reveals Vitamin D-Binding Protein (VDBP) as a Potential Biomarker for Low Bone Mineral Density in Mexican Postmenopausal Women." Nutrients 11, no. 12 (2019): 2853. http://dx.doi.org/10.3390/nu11122853.

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Osteoporosis is a skeletal disease mainly affecting women over 50 years old and it represents a serious public health problem because of the high socioeconomic burden. This disease is characterized by deterioration of bone microarchitecture, low bone mineral density (BMD), and increased risk of fragility fractures. This study aimed to identify serum useful proteins as biomarkers for the diagnosis and/or prognosis of osteoporosis and fracture risk. We collected 446 serum samples from postmenopausal women aged ≥45 years old. Based on the BMD measurement, we classified the participants into three
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Insenser, María, Nuria Vilarrasa, Joan Vendrell, and Héctor F. Escobar-Morreale. "Remission of Diabetes Following Bariatric Surgery: Plasma Proteomic Profiles." Journal of Clinical Medicine 10, no. 17 (2021): 3879. http://dx.doi.org/10.3390/jcm10173879.

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Bariatric surgery restores glucose tolerance in many, but not all, severely obese subjects with type 2 diabetes (T2D). We aimed to evaluate the plasma protein profiles associated with the T2D remission after obesity surgery. We recruited seventeen women with severe obesity submitted to bariatric procedures, including six non-diabetic patients and eleven patients with T2D. After surgery, diabetes remitted in 7 of the 11 patients with T2D. Plasma protein profiles at baseline and 6 months after bariatric surgery were analyzed by two-dimensional differential gel electrophoresis (2D-DIGE) and matri
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von Löhneysen, Katharina, Thomas M. Scott, Katrin Soldau, Xiuling Xu, and Jeffrey S. Friedman. "Assessment of the Red Cell Proteome of Young Patients with Unexplained Hemolytic Anemia by Two-Dimensional Differential In-Gel Electrophoresis (DIGE)." PLoS ONE 7, no. 4 (2012): e34237. http://dx.doi.org/10.1371/journal.pone.0034237.

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Thamaga, L., O. Ruzvidzo, and T. B. Dikobe. "Morphological and Proteomic Evaluation of Zea Mays in Response to Osmotic Stress." Open Biotechnology Journal 15, no. 1 (2021): 19–26. http://dx.doi.org/10.2174/1874070702115010019.

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Introduction: Drought is the main abiotic stress responsible for crop loss worldwide. Maize (Zea mays L.) is a widely grown drought-sensitive crop used as a staple food by the growing population. Therefore, it is imperative to assess the molecular mechanisms behind drought response and tolerance in maize. Transcriptomic profiling of abiotic stress responsive pathways in various crops appeared to be an unreliable approach due to post-transcriptional modifications, while there is limited published data on molecular mechanisms of osmotic-stress response in maize. Hence our study aimed at profilin
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Martin, Petra, Sinead Noonan, Blathnaid Nolan, et al. "Characterization of serum proteome in patients with metastatic colorectal cancer responsive and nonresponsive to bevacizumab using 2 d-DIGE." Journal of Clinical Oncology 30, no. 4_suppl (2012): 484. http://dx.doi.org/10.1200/jco.2012.30.4_suppl.484.

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484 Background: Treatment of patients with metastatic colorectal cancer includes chemotherapy and a monoclonal antibody (cetuximab or bevacizumab). Patients who have k-ras mutated tumors are given bevacizumab. However, no biomarker exists to determine those patients who will respond to this targeted treatment. The objective of this study was to investigate the differential protein expression between patients who do and do not respond to bevacizumab and also compare this with normal controls. Methods: Serum from 24 patients diagnosed with metastatic colorectal cancer and 11 normal controls were
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Glancy, Brian, and Robert S. Balaban. "Protein composition and function of red and white skeletal muscle mitochondria." American Journal of Physiology-Cell Physiology 300, no. 6 (2011): C1280—C1290. http://dx.doi.org/10.1152/ajpcell.00496.2010.

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Red and white muscles are faced with very different energetic demands. However, it is unclear whether relative mitochondrial protein expression is different between muscle types. Mitochondria from red and white porcine skeletal muscle were isolated with a Percoll gradient. Differences in protein composition were determined using blue native (BN)-PAGE, two-dimensional differential in gel electrophoresis (2D DIGE), optical spectroscopy, and isobaric tag for relative and absolute quantitation (iTRAQ). Complex IV and V activities were compared using BN-PAGE in-gel activity assays, and maximal mito
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Futcher, B., G. I. Latter, P. Monardo, C. S. McLaughlin, and J. I. Garrels. "A Sampling of the Yeast Proteome." Molecular and Cellular Biology 19, no. 11 (1999): 7357–68. http://dx.doi.org/10.1128/mcb.19.11.7357.

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ABSTRACT In this study, we examined yeast proteins by two-dimensional (2D) gel electrophoresis and gathered quantitative information from about 1,400 spots. We found that there is an enormous range of protein abundance and, for identified spots, a good correlation between protein abundance, mRNA abundance, and codon bias. For each molecule of well-translated mRNA, there were about 4,000 molecules of protein. The relative abundance of proteins was measured in glucose and ethanol media. Protein turnover was examined and found to be insignificant for abundant proteins. Some phosphoproteins were i
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Zhuang, Zhengping, Meng Qi, Jie Li, et al. "Proteomic identification of glutamine synthetase as a differential marker for oligodendrogliomas and astrocytomas." Journal of Neurosurgery 115, no. 4 (2011): 789–95. http://dx.doi.org/10.3171/2011.5.jns11451.

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Object Astrocytomas and oligodendrogliomas are primary CNS tumors that remain a challenge to differentiate histologically because of their morphological variability and because there is a lack of reliable differential diagnostic markers. To identify proteins that are differentially expressed between astrocytomas and oligodendrogliomas, the authors analyzed the proteomic expression patterns and identified uniquely expressed proteins in these neoplasms. Methods Proteomes of astrocytomas and oligodendrogliomas were analyzed using 2D gel electrophoresis and subsequent computerized gel analysis to
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