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Relevant bibliographies by topics / 2D-Gel electrophoresis

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Academic literature on the topic '2D-Gel electrophoresis'

Author: Grafiati

Published: 4 June 2021

Last updated: 15 February 2022

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Journal articles on the topic "2D-Gel electrophoresis"

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Kaczmarek, K., B. Walczak, S. de Jong, and B. G. M. Vandeginste. "Matching 2D Gel Electrophoresis Images." Journal of Chemical Information and Computer Sciences 43, no. 3 (2003): 978–86. http://dx.doi.org/10.1021/ci0256337.

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Winz, M. L., K. Rohr, and S. Wörz. "Geometric Alignment of 2D Gel Electrophoresis Images." Methods of Information in Medicine 48, no. 04 (2009): 320–23. http://dx.doi.org/10.3414/me9229.

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Summary Objectives: 2D gel electrophoresis (2-DE) is the method of choice for analyzing protein expression in the field of proteomics, for example, comparing a reference with a test population. However, due to complex physical and chemical processes the locations of proteins generally vary in different 2-DE images. To cope with these variations, accurate geometric alignment of 2-DE images is important. Methods: We introduce a new elastic registration approach for 2-DE images, which is based on an analytic solution of the Navier equation using Gaussian elastic body splines (GEBS). With this approach cross-effects in elastic deformations can be handled, which is important for the registration of 2-DE images. In addition, landmark correspondences can be included to aid the registration in regions which are difficult to register using intensity information alone. Results: We have successfully applied our approach to register 2-DE gel images of different levels of complexity. In each case, gel images from a reference group are compared with a test group. To analyze the performance of our approach, we have carried out a quantitative evaluation of the registration results. Moreover, we have performed an experimental comparison with a previous elastic registration scheme. Conclusions: From the results we found that our approach is well-suited for the registration of 2-DE gel images of different levels of complexity and it turned out that the approach is superior to a previous hybrid scheme. Moreover, our approach is well-suited in a fully automatic setting and the performance can further be improved when landmark correspondences are available.

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Ji, H. "Preparation of Eukaryotic Lysates for 2D Gel Electrophoresis." Cold Spring Harbor Protocols 2006, no. 28 (2006): pdb.prot4571. http://dx.doi.org/10.1101/pdb.prot4571.

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Dlaska, Margit, Conrad Anderl, Wolfgang Eisterer, and Oliver E. Bechter. "Detection of Circular Telomeric DNA without 2D Gel Electrophoresis." DNA and Cell Biology 27, no. 9 (2008): 489–96. http://dx.doi.org/10.1089/dna.2008.0741.

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Horgan, Graham W. "Sample Size and Replication in 2D Gel Electrophoresis Studies." Journal of Proteome Research 6, no. 7 (2007): 2884–87. http://dx.doi.org/10.1021/pr070114a.

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Kaczmarek, K., B. Walczak, S. de Jong, and B. G. M. Vandeginste. "Feature Based Fuzzy Matching of 2D Gel Electrophoresis Images." Journal of Chemical Information and Computer Sciences 42, no. 6 (2002): 1431–42. http://dx.doi.org/10.1021/ci020266k.

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Natale, Massimo, Bernardetta Maresca, Paolo Abrescia, and Enrico M. Bucci. "Image Analysis Workflow for 2-D Electrophoresis Gels Based on ImageJ." Proteomics Insights 4 (January 2011): PRI.S7971. http://dx.doi.org/10.4137/pri.s7971.

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A number of commercial software packages are currently available to perform digital two-dimensional electrophoresis (2D-GE) gel analysis. However, both the high cost of the commercial packages and the unavailability of a standard data analysis workflow, have prompted several groups to develop freeware systems to perform certain steps of gel analysis. Unfortunately, to the best of our knowledge none of them offer a package that performs all the steps envisaged in a 2D-GE gel analysis. Here we describe an ImageJ-based procedure, able to manage all the steps of a 2D-GE gel analysis. ImageJ is a free available image processing and analysis application developed by National Institutes of Health (NIH) and widely used in different life sciences fields as medical imaging, microscopy, western blotting and PAGE. Nevertheless no one has yet developed a procedure enabled to compare spots on 2D-GE gels. We collected all used ImageJ tools in a plug-in that allows us to perform the whole 2D-GE analysis. To test it, we performed a set of 2D-GE experiments on plasma samples from 9 patients victims of acute myocardial infarction and 8 controls, and we compared the results obtained by our procedure to those obtained using a widely diffuse commercial package, finding similar performances.

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Johnson, D. Thor, Robert A. Harris, Stephanie French, Angel Aponte, and Robert S. Balaban. "Proteomic changes associated with diabetes in the BB-DP rat." American Journal of Physiology-Endocrinology and Metabolism 296, no. 3 (2009): E422—E432. http://dx.doi.org/10.1152/ajpendo.90352.2008.

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These studies were structured with the aim of utilizing emerging technologies in two-dimensional (2D) gel electrophoresis and mass spectrometry to evaluate protein expression changes associated with type 1 diabetes. We reasoned that a broad examination of diabetic tissues at the protein level might open up novel avenues of investigation of the metabolic and signaling pathways that are adversely affected in type 1 diabetes. This study compared the protein expression of the liver, heart, and skeletal muscle of diabetes-prone rats and matched control rats by semiquantitative liquid chromatography-mass spectrometry and differential in-gel 2D gel electrophoresis. Differential expression of 341 proteins in liver, 43 in heart, and 9 (2D gel only) in skeletal muscle was detected. These data were assembled into the relevant metabolic pathways affected primarily in liver. Multiple covalent modifications were also apparent in 2D gel analysis. Several new hypotheses were generated by these data, including mechanisms of net cytosolic protein oxidation, formaldehyde generation by the methionine cycle, and inhibition of carbon substrate oxidation via reduction in citrate synthase and short-chain acyl-CoA dehydrogenase.

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Xavier, Ilungo J., and George G. Khachatourians. "Heat-shock response of the entomopathogenic fungus Beauveria brongniartii." Canadian Journal of Microbiology 42, no. 6 (1996): 577–85. http://dx.doi.org/10.1139/m96-078.

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The heat-shock response of five strains of the entomopathogenic fungus Beauveria brongniartii was studied using two-dimensional (2D) gel electrophoresis. The fungal cells were heat shocked at 45 °C for 1 h and the total cellular protein was subjected to 2D gel electrophoresis. Proteins were separated in the first dimension using isoelectric focusing (pH range of 3.0–10) and in the second dimension by sodium dodecyl sulphate – polyacrylamide gel electrophoresis. More than 150 polypeptides for each strain were visualized by silver staining and have been assigned individual numbers as polypeptide coordinates. Analysis of the polypeptide map obtained by 2D gels indicated three patterns; several unique heat-shock proteins (HSPs) were (i) induced, (ii) enhanced, or (iii) repressed. Some of the HSPs induced by 45 °C were unique for each of the strains tested. Identification of heat-inducible protein synthesis or repression has ramifications for field survival and performance of entomopathogenic fungi. As well, the HSPs can be used as "signature proteins" for identification pruposes and this raises the possibility of using HSPs as a diagnostic tool applicable to other pest control fungi.Key words: heat-shock proteins, heat-shock response, two-dimensional electrophoresis, entomopathogenic fungi, Beauveria brongniartii.

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Cheng, Hao-Tsai, Sen-Yung Hsieh, Chang-Mu Sung, Betty Chien-Jung Pai, Nai-Jen Liu, and Carl PC Chen. "Optimizing Human Bile Preparation for Two-Dimensional Gel Electrophoresis." BioMed Research International 2016 (2016): 1–6. http://dx.doi.org/10.1155/2016/5185317.

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Aims. Bile is an important body fluid which assists in the digestion of fat and excretion of endogenous and exogenous compounds. In the present study, an improved sample preparation for human bile was established.Methods and Material. The method involved acetone precipitation followed by protein extraction using commercially available 2D Clean-Up kit. The effectiveness was evaluated by 2-dimensional electrophoresis (2DE) profiling quality, including number of protein spots and spot distribution.Results. The total protein of bile fluid in benign biliary disorders was 0.797 ± 0.465 μg/μL. The sample preparation method using acetone precipitation first followed by 2D Clean-Up kit protein extraction resulted in better quality of 2DE gel images in terms of resolution as compared with other sample preparation methods. Using this protocol, we obtained approximately 558 protein spots on the gel images and with better protein spots presentation of haptoglobin, serum albumin, serotransferrin, and transthyretin.Conclusions. Protein samples of bile prepared using acetone precipitation followed by 2D Clean-Up kit exhibited high protein resolution and significant protein profile. This optimized protein preparation protocol can effectively concentrate bile proteins, remove abundant proteins and debris, and yield clear presentation of nonabundant proteins and its isoforms on 2-dimensional electrophoresis gel images.

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Dissertations / Theses on the topic "2D-Gel electrophoresis"

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Chan, Hong-Lin. "A 2D-difference gel electrophoresis strategy for redox proteomics." Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1444604/.

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Post-genomic biomedical science requires quantitative proteomics. In most cases this involves differential protein expression analysis using matched pairs of simultaneously detectable labelling reagents for specific protein amino acids. In this thesis the development and optimisation of a novel cysteine labelling strategy, that is based on the use of iodoacetyl derivatives of Cy3 and Cy5 (ICy3/5) and 2D-difference gel electrophoresis (2D-DIGE) is described. The differentially labelled samples are separated on a single 2D gel and detected by multi-wavelength fluorescence scanning. The method is used to analyse standard proteins and then cell lysates to define the stoichiometry, sensitivity and specificity of this labelling technique. A comparative study of this new proteomic ICy dye protocol with the current NHS-Cy dye labelling system and methods that employ commonly used protein staining methods is described. The method is then used for cysteine labelling of proteins in non-reduced, denatured biological samples allowing accurate monitoring and sensitive detection of redox-dependent thiol modifications and expression level changes. The method is shown to be compatible with the use of MALDI mass spectrometry to identify proteins by analysis of trypsinised ICy labelled peptide digests. Using parallel sample analysis within single gels, the ICy-dye reagents were used to detect redox-, ErbB-2- and growth factor-dependent changes in a human mammary luminal epithelial cell system which was exposed to hydrogen peroxide or to growth factor stimulation. The conventional lysine labelling 2D-DIGE technique was also used in parallel to assess the new ICy labelling strategy for determination of the effects of oxidative stress on protein isoform levels. This study has revealed the identity of proteins involved in the response to oxidative stress and growth factor stimulation in the context of ErbB-2 growth factor receptor over-expression. In addition, this labelling strategy was also used to detect changes in thiol reactivity that follow the UV irradiation of plasma proteins as part of a study designed to evaluate the effect of UV disinfection on plasma product safety for clinical use.

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Brown, Andrew S. "Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Characterization of Decorin." Youngstown State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311873768.

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Pandey, Archana. "Proteome analysis of Pseudomonas putida KT2440 using 2D gel electrophoresis and LC/ESI-Q-TOF mass spectrometry /." Online version of thesis, 2007. https://ritdml.rit.edu/dspace/handle/1850/3848.

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Šopíková, Martina. "Změny proteinového profilu v průběhu sladování ječmene." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216437.

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This diploma thesis is focused on studies of changing of protein profile during barley malting. Substantial part of this work is devoted to the proteomics identification of barley proteins which change during malting and so become more stationary and they influence quality of beer (haze and foam in beer). For this experiment was used barley variety Jersey. In the theoretical part of this thesis there is information about beer, manufacturing of beer with description of important commodities for manufacturing of beer and information about barley malting and information about malting process. Next there is description of methods for separation of proteins (1D gel electrophoresis and 2D gel electrophoresis), MALDI TOF/TOF mass spectrometry and this use for the analysis and identification of proteins, the use of matrices and ways of the sample preparation. In the experimental part of this thesis there was carried out the optimisation of the dosage of sample for 1D gel electrophoresis and the optimisation of staining. The 15 % TRIS-HCl gel was the best, this gel was stained by Commassie Brilliant Blue G-250. For illustration of changes was made 2D gel electrophoresis. With help of method peptide mass fingerprinting and MS/MS protein of barley – protein Z, -amylase subtilisin inhibitor, -amylase a peroxidase were identificated. The analysis of barley extract intact proteins was carried out, this analysis was focused on changes of important barley protein LTP 1.

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Bartee, Eric Carter. "Discovery and characterization of a novel family of human ubiquitin ligases termed Membrane Associated RING-CH (MARCH) proteins." Oregon Health & Science University, 2007. http://content.ohsu.edu/u?/etd,629.

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Ph.D.<br>Molecular Microbiology and Immunology<br>Both poxviruses and γ2-herpesviruses share the K3-family of viral immune evasion proteins. These proteins are characterized by an amino-terminal RING-CH domain followed by two transmembrane domains. We analyzed several human homologues of the K3-family termed membrane-associated RING-CH (MARCH) proteins. All MARCH proteins localized to subcellular membranes while several reduced surface levels of known K3-family substrates. Thus, MARCH proteins appear to be structurally and functionally homologous to viral K3 proteins. One of the major challenges in determining the function of this family is the identification of their physiological substrates. To overcome this we created a quantitative proteomics approach which can be used to identify novel substrates for both the K3- and MARCH-families. Using stable isotope labeling by amino acids in cell culture, we compared the proteome of plasma membrane, golgi, and endoplasmic reticulum membranes in the presence and absence of K5 and MARCH-VIII. Quantitative mass spectrometric protein identification from these fractions revealed that CD316 (bone marrow stromal antigen 2), CD166 (activated leukocyte cell adhesion molecule) and syntaxin-4 were consistently underrepresented in the plasma membrane of K5 expressing cells, while CD44, CD81 (TAPA-1) and B-cell receptor-associated protein 31kDa (Bap31) were consistently underrepresented in the plasma membrane of MARCH-VIII expressing cells. Furthermore, downregulation of each of these proteins was independently confirmed. Our results both identify and characterize a novel family of human ubiquitin ligase enzymes and elucidate a novel technique which can analyze this family and be easily adapted to the analysis of other cellular enzymes viral immune modulators.

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Guterres, Sheila Barreto. "Busca de biomarcadores para esquizofrenia em plaquetas utilizando eletroforese diferencial em gel bidimensional (2D-DIGE) e espectrometria de massas." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/75/75132/tde-16112011-150931/.

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A esquizofrenia é uma doença crônica, grave e incapacitante que afeta cerca 24 milhões de pessoas em âmbito mundial. É caracterizada por uma desorganização no pensamento que prejudica a funcionalidade do indivíduo. Existem intervenções que são efetivas e contribuem para a diminuição da prevalência do transtorno, pois ajudam o portador a levar uma vida produtiva e integrada à sociedade, porém devem ser ministradas nos estágios iniciais da doença. No entanto, existe uma grande dificuldade em se diagnosticar a esquizofrenia precocemente devido a sua complexidade e às sutilezas dos seus sintomas apresentados antes do surgimento da psicose. O cérebro não é acessível a exames invasivos in vivo e por esse motivo a exploração de fluidos periféricos é de grande importância. As plaquetas e neurônios serotonérgicos possuem características bioquímicas e morfológicas em comum que possibilitam a comparação entre a estrutura e a função de ambos e, por causa dessa similaridade, muitos trabalhos utilizam plaquetas como modelo para o estudo de doenças neuropsiquiátricas, inclusive a esquizofrenia. A detecção precoce da esquizofrenia é um objeto de investigação atual e relevante não somente para revolucionar os meios atuais de diagnóstico, mas também para desenvolver novos tratamentos aplicados aos estágios iniciais da doença, diferenciar os subgrupos de doentes e monitorar as intervenções preventivas. A proposta do presente trabalho é fazer o estudo da expressão de proteínas em plaquetas de pacientes esquizofrênicos e controles com o objetivo de identificar proteínas candidatas a biomarcadores utilizando técnicas proteômicas quantitativas e confiáveis, como 2D-DIGE e a espectrometria de massas.<br>Schizophrenia is a disabling, serious, and chronic illness, which affects about 24 million people worldwide. It is characterized by a severe disorganization of the thoughts that harms the social life of patients becoming them dependent of the family and/or government. There are effective treatments that contribute to decrease the prevalence of the disorder because they improve the life and social conditions of the patients, but they are only advantageous if the intervention is made in the early stages of the disease. It is difficult to obtain early diagnosis due to the complexity of the disease and its insidious symptoms before the beginning of the psychosis. The brain is not easily accessed in vivo and, because of this, it is very important to study the peripheral tissues like blood, which makes the use of the platelets very interesting. Furthermore, platelets and serotonergic neurons share biochemical and morphological characteristics that allows the comparison between structure and function of both. From these similarities many authors has used platelets as a neuron model to study many neurodegenerative diseases including schizophrenia. The early detection of schizophrenia is a current and suitable goal, not only to improve the early diagnosis but also to develop new treatments, differentiate the subtypes, and monitor the preventive interventions. The purpose of this project is to do a comparative screening of expressed proteins in platelets from schizophrenics and controls with the objective of finding differently expressed proteins that could be candidates to biomarkers using 2D-DIGE and mass spectrometry.

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Woolard, Christopher Lee. "Identification of Potential Protein Biomarkers of Low Level Kidney Degradation." Wright State University / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=wright1247498817.

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Leung, Man Ching. "Identification of human hair follicle antigens targeted in the presumptive autoimmune hair follicle disorder Alopecia Areata and their potential functional relevance In Vitro. Methods development for isolation and identification of Alopecia Areata-relevant human hair follicle antigens using a proteomics approach and their functional assessment using an Ex Vivo hair follicle organ culture model." Thesis, University of Bradford, 2008. http://hdl.handle.net/10454/4330.

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Alopecia areata (AA) is a putative autoimmune hair loss disorder. It mainly affects the scalp hair but can also involve body hair, and can also affect the nail and the eye. While there are may be several lines of evidence to support the autoimmune basis of AA, there is still very little information on the hair follicle autoantigen(s) involved in its pathogenesis. In this project, serum antibodies (AA=10, control=10) were used to immunoprecipitate AA-relevant target antigens from normal human scalp hair follicle extracts. These immunoprecipitates were analysed by LC-MALDI-TOF/TOF mass spectrometry for target protein identification. This part of the project involved substantial methods development. Trichohyalin was immunoprecipitated by all AA sera, but by only 5 normal sera. Importantly, the mean Mascot scores of the AA group was significantly higher than the normal group (p=0.005). Keratin 16 was also identified from immunoprecipitates as another potential AA-relevant target antigen. Functional studies by ex vivo whole hair follicle organ culture using commercial antibodies to trichohyalin and keratin 16 significantly inhibited hair fibre elongation compared to controls. Indirect immunofluorescence studies revealed that AA sera contained higher immunoreactivity against normal human scalp anagen hair follicles compared to normal sera. Immunoreactivities were mainly in the outer root sheath and inner root sheath, and less so to the medulla and hair bulb matrix. Double immunofluorescence studies of AA and normal serum with anti-trichohyalin antibody (AE15) revealed co-localisation of 9 of the AA sera antibodies with trichohyalin in the inner root sheath (mostly in Henle's, less in Huxley's/inner root sheath cuticle), but only weakly in 3 normal sera. This study supports the involvement of an antibody response to anagen-specific hair follicles antigens in AA. Moreover, there may be some evidence that these antibodies may have a pathogenic role.

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Muchindu, Munkombwe. "Electrochemical ochratoxin a immunosensors based on polyaniline nanocomposites templated with amine- and sulphate-functionalised polystyrene latex beads." Thesis, University of the Western Cape, 2010. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_3815_1306752491.

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<p>Polyaniline nanocomposites doped with poly(vinylsulphonate) (PV-SO3 &minus<br>) and nanostructured polystyrene (PSNP) latex beads functionalized with amine (PSNP-NH2) and sulphate (PSNP-OSO3 &minus<br>) were prepared and characterised for use as nitrite electro-catalytic chemosensors and ochratoxin A immunosensors. The resultant polyaniline electrocatalytic chemosensors (PANI, PANI|PSNP-NH2 or PANI|PSNP-OSO3 &minus<br>) were characterized by cyclic voltammetry (CV), ultraviolet-visible (UV-Vis) spectroscopy and scanning electron microscopy (SEM). Brown-Anson analysis of the multi-scan rate CV responses of the various PANI films gave surface concentrations in the order of 10&minus<br>8 mol/cm. UV-vis spectra of the PANI films dissolved in dimethyl sulphoxide showed typical strong absorbance maxima at 480 and 740 nm associated with benzenoid p-p* transition and quinoid excitons of polyaniline, respectively. The SEM images of the PANI nanocomposite films showed cauliflower-like structures that were &lt<br>100 nm in diameter. When applied as electrochemical nitrite sensors, sensitivity values of 60, 40 and 30 &mu<br>A/mM with corresponding limits of detection of 7.4, 9.2 and 38.2 &mu<br>M NO2 &minus<br>, were obtained for electrodes, PANI|PSNP-NH2, PANI and PANI|PSNP-SO3 &minus<br>, respectively. Immobilisation of ochratoxin A antibody onto PANI|PSNP-NH2, PANI and PANI|PSNPSO3 - resulted in the fabrication of immunosensors.</p>

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Kierul, Kinga. "Comprehensive proteomic study of Bacillus amyloliquefaciens strain FZB42 and its response to plant root exudates." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://dx.doi.org/10.18452/16805.

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Bacillus amyloliquefaciens FZB42 ist ein frei lebendes Bakterium, das Pflanzenwurzeln besiedelt und das Pflanzenwachstum durch viele verschiedene Wirkmechanismen anregt. In dieser Arbeit wurden die molekularen Grundlagen dieser positiven Wirkungen, die dieses „Pflanzenwachstum fördernde Rhizobakterium“ (PGPR) auf seine Wirte ausübt, untersucht. Um den gegenseitigen Austausch von B. amyloliquefaciens und seinen Wirtspflanzen zu entschlüsseln, wurden umfangreiche Proteomstudien durchgeführt. Es wurden Referenzkarten der extrazellulären und zytosolischen Proteinfraktionen erstellt. Die größte Anzahl an ausgeschiedenen Proteinen konnte während der stationären Phase beobachtet werden. Die identifizierten extrazellulären Proteine gehören verschiedenen Funktionsklassen an, wobei die prominentesten Klassen am Kohlenhydrat-Abbau und den Transport von Molekülen durch die Zellwand beteiligt sind. Die zytosolischen Extrakte von Kulturen, die in 1C-Medium bzw. Mineralmedium angezogen wurden, und in der zweidimensionalen Gelelektrophorese (2 DE) aufgetrennt wurden, ergaben 461 und 245 verschiedene Protein-Einträge. Die erstellten Referenz-Karten wurden anschließend verwendet, um Proteine und Prozesse, in an der Interaktion mit Pflanzen beteiligt sind, zu identifizieren. Dafür wurden die Bakterien Wurzelexudaten von Mais (Zea mays L.) ausgesetzt. Die Proteine aus zwei Stämmen, denen die globalen Transkriptionsregulatoren (Degu, AbrB) und vier Sigma-Faktoren (SigB, SigM, SigV, und SigX) fehlen, wurden ebenfalls untersucht, um ihre Beteiligung an den bakteriellen Reaktionen auf die Wurzelausscheidungen zu analysieren. Zusammenfassend ist dies die erste Studie, die umfangreiche Proteomdaten von Gram-positiven PGPR präsentiert, wobei gleichzeitig die Veränderung der Expression von extrazellulären und zytoplasmatischen Proteinen, nach Zugabe von Wurzelexudaten, ausgewertet wurde.<br>Bacillus amyloliquefaciens strain FZB42 is a free-living bacterium that competitively colonizes plant roots and stimulates plant growth by many different modes of action. The molecular basis of singular beneficial effects that this Plant Growth-Promoting Rhizobacteria (PGPR) exert on their hosts have been studied. To decipher the molecular cross-talk of B. amyloliquefaciens and its’ host plants as a whole system, an extensive proteomic approach was performed. Reference maps of the extracellular and cytosolic protein fractions were established. The highest number of secreted proteins was observed during stationary growth phase. Identified extracellular proteins belong to different functional classes, with the most prominent classes involved in carbohydrate degradation and transportation of molecules across the cell wall. Cytosolic extracts obtained from cultures grown in 1C and minimal media subjected to the 2 Dimensional Electrophoresis (2 DE), revealed 461 and 245 different protein entries, respectively. Created reference maps were subsequently used to identify proteins and processes involved in the interaction with plants, prior to exposure of bacteria to maize (Zea mays L.) root exudates. The proteomics of two strains lacking expression of genes coding for global transcriptional regulators (degU, abrB) and four sigma factors (sigB, sigM, sigV, and sigX) were also inves-tigated, in order to analyse their involvement in bacterial responses to root exudates. In summary, this is the first study presenting comprehensive proteomics of Gram-positive PGPR, evaluating at the same time changes in protein expression caused by addition of root exudates at the extracellular and cytosolic level.

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Books on the topic "2D-Gel electrophoresis"

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Anton, Posch, ed. 2D PAGE: Sample preparation and fractionation. Humana Press, 2008.

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2D PAGE: Sample Preparation and Fractionation: Volume 1 (Methods in Molecular Biology). Humana Press, 2008.

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Posch, Anton. 2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology). Humana Press, 2008.

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Book chapters on the topic "2D-Gel electrophoresis"

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Meleady, Paula. "Two-Dimensional Gel Electrophoresis and 2D-DIGE." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7268-5_1.

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Naula, Christina, and Richard Burchmore. "2D Gel Electrophoresis Analysis of Leishmania Proteomes." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0294-2_34.

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Xia, Xuhua. "Bioinformatics and In Silico 2D Gel Electrophoresis." In Bioinformatics and the Cell. Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-90684-3_18.

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Simula, Maria Paola, Agata Notarpietro, Giuseppe Toffoli, and Valli De Re. "2-D Gel Electrophoresis: Constructing 2D-Gel Proteome Reference Maps." In Methods in Molecular Biology. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-61779-424-7_13.

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Freeman, Lita A. "Native–Native 2D Gel Electrophoresis for HDL Subpopulation Analysis." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-60327-369-5_17.

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Rabilloud, Thierry. "How to Use 2D Gel Electrophoresis in Plant Proteomics." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-631-3_4.

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Marczyk, Michal. "Processing 2D Gel Electrophoresis Images for Efficient Gaussian Mixture Modeling." In Advances in Intelligent Systems and Computing. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-60816-7_5.

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Viterbo, David, and Guy-Franck Richard. "Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9784-8_4.

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Meleady, Paula. "2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-289-2_9.

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Marczyk, Michal. "Improved Detection of 2D Gel Electrophoresis Spots by Using Gaussian Mixture Model." In Bioinformatics Research and Applications. Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-38782-6_24.

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Conference papers on the topic "2D-Gel electrophoresis"

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Wang, Weixing. "Spot identification on 2D electrophoresis gel images." In Fourth International Conference on Photonics and Imaging in Biology and Medicine, edited by Kexin Xu, Qingming Luo, Da Xing, Alexander V. Priezzhev, and Valery V. Tuchin. SPIE, 2006. http://dx.doi.org/10.1117/12.710884.

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Kostopoulou, Eirini, Eleni Zacharia, and Dimitris Maroulis. "Detection and segmentation in 2D gel electrophoresis images." In 2011 17th International Conference on Digital Signal Processing (DSP). IEEE, 2011. http://dx.doi.org/10.1109/icdsp.2011.6004967.

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Hoang, Minh-Tuan T., Hieu T. Huynh, Nguyen H. Vo, Yonggwan Won, and Jung-Ja Kim. "Two-Step Iterative Registration for 2D-Gel Electrophoresis Images." In 2007 IEEE International Conference on Research, Innovation and Vision for the Future. IEEE, 2007. http://dx.doi.org/10.1109/rivf.2007.369168.

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Desai, Shrinivas D., and S. D. Savitha. "Seeded Watershed Segmentation Based Proteomics for 2D-Gel Electrophoresis Images." In the Third International Symposium. ACM Press, 2015. http://dx.doi.org/10.1145/2791405.2791449.

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Zacharia, Eleni, Eirini Kostopoulou, Dimitris Maroulis, and Sophia Kossida. "A Spot Segmentation Approach for 2D Gel Electrophoresis Images Based on 2D Histograms." In 2010 20th International Conference on Pattern Recognition (ICPR). IEEE, 2010. http://dx.doi.org/10.1109/icpr.2010.622.

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Hoeflich, Christopher S., and Jason J. Corso. "Segmentation of 2D gel electrophoresis spots using a Markov random field." In SPIE Medical Imaging, edited by Josien P. W. Pluim and Benoit M. Dawant. SPIE, 2009. http://dx.doi.org/10.1117/12.811802.

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Worz, Stefan, Marie-Luise Winz, and Karl Rohr. "Geometric alignment of 2D gel electrophoresis images using physics-based elastic registration." In 2008 5th IEEE International Symposium on Biomedical Imaging (ISBI 2008). IEEE, 2008. http://dx.doi.org/10.1109/isbi.2008.4541201.

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"A NOVEL GAUSSIAN FITTING APPROACH FOR 2D GEL ELECTROPHORESIS SATURATED PROTEIN SPOTS." In International Conference on Bioinformatics Models, Methods and Algorithms. SciTePress - Science and and Technology Publications, 2012. http://dx.doi.org/10.5220/0003789803350338.

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Ijaz, Umer Zeeshan, Safee Ullah Chaudhary, Moon Sang Don, and Kyung Youn Kim. "Computational Strategies for Protein Quantitation in 2D Electrophoresis Gel Image Processor for Matlab." In 2007 Frontiers in the Convergence of Bioscience and Information Technologies. IEEE, 2007. http://dx.doi.org/10.1109/fbit.2007.95.

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Sudhakara, Sagar, Nikunj Patel, and V. M. Gadre. "Non-subsampled contourlet transform & coherent point drift based registration of 2D gel electrophoresis images." In 2017 2nd International Conference for Convergence in Technology (I2CT). IEEE, 2017. http://dx.doi.org/10.1109/i2ct.2017.8226162.

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