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Dissertations / Theses on the topic '2D-PAGE'

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Published: 4 June 2021

Last updated: 25 July 2025

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1

Brown, Andrew S. "Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Characterization of Decorin." Youngstown State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311873768.

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2

Queiroz, João Vitor de. "Estratégias bioanalíticas para caracterização de biomarcadores de exposição ao mercúrio em Arapaima gigas e Serrasalmus rhombeus do rio Madeira/bacia amazônica." Botucatu, 2017. http://hdl.handle.net/11449/150276.

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Orientador: Pedro de Magalhães Padilha<br>Resumo: Este trabalho apresenta os resultados de proteínas associadas ao mercúrio em amostras de tecido muscular e hepático de pirarucu (Arapaima gigas) e piranha preta (Serrasalmus rhombeus) oriundos do complexo hidrelétrico de Jirau, na Bacia do Rio Madeira, região amazônica do Brasil. O proteôma do músculo e fígado dessas espécies foi obtido por eletroforese bidimensional em gel de poliacrilamida (2D PAGE). O mercúrio presente nos spots proteicos foi determinado por espectrometria de absorção atômica em forno de grafite (GFAAS) após mineralização ác

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3

Cerbino, Maria Rosa. "ABORDAGEM METALOPROTEÔMICA DO MERCÚRIO EM LEITE MATERNO DE COMUNIDADES DA BACIA AMAZÔNICA - BRASIL." Pontifícia Universidade Católica de Goiás, 2016. http://tede2.pucgoias.edu.br:8080/handle/tede/3556.

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Submitted by admin tede (tede@pucgoias.edu.br) on 2016-11-30T18:10:51Z No. of bitstreams: 1 MARIA ROSA CERBINO.pdf: 2627014 bytes, checksum: 98680184bb60e636de01507e3f1eeec4 (MD5)<br>Made available in DSpace on 2016-11-30T18:10:51Z (GMT). No. of bitstreams: 1 MARIA ROSA CERBINO.pdf: 2627014 bytes, checksum: 98680184bb60e636de01507e3f1eeec4 (MD5) Previous issue date: 2016-04-12<br>Mercury is a potentially toxic element with a wide distribution on the Amazonian environment. This metal is dangerous and responsible for environmental contaminations and human intoxications as it is capable to bi

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4

Nadaf, Somayyeh. "Analyse protéomique et transcriptomique de la maturation folliculaire." Thesis, Tours, 2010. http://www.theses.fr/2010TOUR4037.

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La compréhension des mécanismes cellulaires et moléculaires qui sont mis en jeu au moment de la croissance et la maturation pré-ovulatoire induite par la LH, permettra de définir des marqueurs de qualité et de maturité du follicule destiné à ovuler, et ainsi de mieux anticiper le moment de l’ovulation. L’objectif majeur de cette thèse était d’identifier certain des facteurs régulateur impliqués dans la maturation folliculaire par deux approches globales d’analyse protéomique et transcriptomique. La première étude a permis d’établir pour la première fois les cartes protéiques du liquide follicu

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Fulton, Benjamin L. "2D-PAGE Analysis of Myocardial Collagen in Male and Female Spontaneously Hypertensive Rats." Connect to resource online, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1219668882.

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6

Schmitz, Gabriela Justamante Handel. "Análise comparativa dos proteomas das raízes tuberosas de mandioca (Manihot esculenta Crantz) de variedades de mesa e indústria." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/9/9131/tde-28082015-154641/.

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A mandioca (Manihot esculenta Crantz) é uma das principais culturas do mundo, havendo grande variabilidade genética. As variedades são classificadas com base na palatabilidade e toxicidade das raízes, em mansas ou doces e bravas ou amargas. Apesar da importância, o potencial da mandioca é pouco explorado, não sendo conhecidos, em nível molecular, os elementos determinantes para as suas características. Assim, pretendeu-se identificar, empregando a 2D-PAGE, proteínas que possam estar associadas com as diferenças físico-químicas das raízes tuberosas de variedades de mesa (IAC 576-70 e IAC 06-01)

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7

MILLI, Alberto. "2D-page coupled to mass spectrometry for proteomic analysis of human, microbial and plant samples." Doctoral thesis, Università degli Studi di Verona, 2009. http://hdl.handle.net/11562/337513.

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Il progetto di dottorato è stato condotto nel Laboratorio di Proteomica del Dipartimento di Biotecnologie dell’Università di Verona. Per la stesura di tale progetto sono state instaurate collaborazioni con alcuni laboratori, sia interni allo stesso Dipartimento sia appartenenti ad altre Università o Istituti di Ricerca. In particolare, per quanto riguarda lo studio su cellule di cancro colorettale trattate con un nuovo chemiofarmaco, il Laboratorio del Prof. Zunino (Fondazione IRCCS, Istituto Nazionale dei Tumori, Milano) ha fornito tutti i campioni biologici, mentre il gruppo del Prof.

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8

Vieira, José Cavalcante Souza. "Investigação de metalotioneínas em peixes da região de Jirau - bacia do Rio Madeira - Rondônia." Botucatu, 2017. http://hdl.handle.net/11449/150736.

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Orientador: Pedro de Magalhães Padilha<br>Resumo: Devido a sua grande concentração de nutrientes, tais como proteínas, vitaminas e minerais, o peixe é considerado um dos alimentos mais saudáveis que se pode encontrar na natureza. No entanto, a ingestão de peixes é considerada a forma predominante de via de exposição do ser humano ao mercúrio (Hg), principalmente para as populações que vivem às margens dos rios, onde o peixe constitui a principal fonte de proteína. Na tentativa de elucidar os mecanismos de toxicidade das espécies mercuriais, o teor desse metal tem sido estudado intensamente pel

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9

Lonardoni, Francesco. "Discovery and quantification of proteins of biological relevance through differential proteomics and biosensing." Thesis, Cranfield University, 2012. http://dspace.lib.cranfield.ac.uk/handle/1826/8676.

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Medical diagnosis is the process of attempting to determine and/or identify a possible disease or disorder. This process is revealed by biomarkers, defined by The Food and Drug Administration (FDA) as “characteristics that are objectively measured and evaluated as indicators of normal biologic processes, pathogenic processes, or pharmacologic responses to a therapeutic intervention”. The process of biomarker discovery has been boosted in the last years by proteomics, a research discipline that takes a snapshot of the entire wealth of proteins in an organism/ tissue/ cell/ body fluid. An implem

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10

Mabiya, Thembeka. "Development of a plum chromosome doubling method and proteomics and biochemical characterization." University of the Western Cape, 2015. http://hdl.handle.net/11394/4875.

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>Magister Scientiae - MSc<br>Chromosome doubling has become an important tool in breeding programmes as it offers the ability of introducing novel traits into existing plants. Doubled haploid plants are highly valued by both consumers and breeders as these plants usually show larger flower, leaves and fruit, thus making them more marketable. Marianna open pollinated plum rootstocks’ adaptability to different soil types and moisture conditions has been favoured in polyploidy studies as parental material in breeding programmes. The potential of the microtubule depolymerizing herbicide (oryzalin)

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11

Mahlatsi, Tladi Abram. "Characterization of heavy metal tolerant bacterial plasmids isolated from a platinum mine tailings dam / by Tladi Abram Mahlatsi." Thesis, North-West University, 2012. http://hdl.handle.net/10394/9780.

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The development of metal-tolerance and antibiotic resistance in bacteria may be caused by metals polluting a particular environment. During mining and mineral processing activities, large quantities of metals are deposited into the soil. These high concentrations of metals are evolutionary pressures selecting for microorganisms tolerant to these metals. Metaltolerance maybe conferred to these organisms by mobile genetic elements such as plasmids. This study describes the characteristics of plasmids isolated from various bacteria that displayed an ability to withstand high metal concentrations.

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RIBEIRO, Isadora Louise Alves da Costa. "Proteômica de cana-de-açúcar em condição de estresse hídrico." Universidade Federal de Pernambuco, 2010. https://repositorio.ufpe.br/handle/123456789/6830.

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Made available in DSpace on 2014-06-12T18:07:42Z (GMT). No. of bitstreams: 2 arquivo785_1.pdf: 5265393 bytes, checksum: 53f0041ef3eeb76392e1850d05f10e31 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2010<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>O aumento da produção de cana-de-açúcar, decorrente da crescente demanda por etanol e biocombustíveis, implica na necessidade de obter variedades mais produtivas e adaptadas a fatores ambientais limitantes, como a seca. Assim, torna-se necessário o conhecimento dos ge

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Okoli, Arinze Stanley Medical Sciences Faculty of Medicine UNSW. "Molecular studies of the response of Helicobacter hepaticus to bile, and the effect of Helicobacter bilis on human hepatoma cells." Publisher:University of New South Wales. Medical Sciences, 2009. http://handle.unsw.edu.au/1959.4/43379.

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Enterohepatic Helicobacter species (EHS) are emerging infectious disease agents. Infection of the enterohepatobiliary tract of several mammals by this group of bacteria results in various pathological disorders. The availability of the Helicobacter hepaticus sequenced and annotated genome, allowed molecular characterisation of the responses of H. hepaticus to host factors such as bile. The adaptation/responses of the bacterium to bovine, porcine and human bile were investigated using proteomics and transcriptomics. Ninety-one different proteins were identified in the responses of H. hepaticus

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14

Iyer, Radhika. "RADIATION INDUCED DIFFERENTIAL EXPRESSION OF PROTEINS IN THE INTESTINE OF EGFR COMPROMISED MICE." Ohio University / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1133463186.

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15

Mofokeng, Henrietta Refiloe. "Identification of a transducin (beta)-like 3 protein as a potential biomarker of prediabetes from rat urine using proteomics." Thesis, University of the Western Cape, 2010. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_4144_1361870602.

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<p>Obesity is a globally increasing disease particularly in developing countries and among children. It is mainly caused by intake of diets high in fat and the lack of physical activity. Obesity is a risk factor for diseases such as type II diabetes, high blood pressure, high cholesterol and certain cancers. Prediabetes is a condition where blood glucose levels are above normal but have not&nbsp<br>reached those of diabetes. It is difficult to diagnose, as there are no signs or symptoms. Some type II diabetes patients bear no symptoms at all and the disease is discovered late. Proteomics is a

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Cakici, Ozgur. "Biochemical And Genetic Characterization Of Halobacterium Salinarium Strain Isolated From Tuz Lake In Central Anatolia." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/3/12604752/index.pdf.

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In this study, a halophilic archaea Halobacterium salinarium TG13 which is isolated from Tuz Lake in Central Anatolia was characterized biochemically and genetically. Halobacterium salinarium DSM3754 and Halobacterium salinarium S9 strains were used as a reference strain through the experiments. In biochemical characterization<br>total protein profiles of strains was compared by using 1D SDS PAGE. Total protein profile of the isolated strain has shown differences. The SDS-PAGE profile of the purified purple membrane showed only single band by coomassie staining. Molecular weight and pI values

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17

Santos, Gabriel de Oliveira. "Indução da ovulação com hcg e acetato de deslorelina altera o perfil proteico do líquido folicular de éguas." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/96909.

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O líquido folicular é o microambiente do oócito durante sua maturação in vivo que é, em parte constituído por exsudato do soro sanguíneo e por substâncias produzidas localmente, que estão relacionados com a atividade metabólica das células ovarianas. Tais substâncias podem ser essenciais para a proliferação e diferenciação das células somáticas bem como na maturação e posterior fertilização de um oócito competente. A busca por biomarcadores capazes de predizer a saúde de um folículo ou a capacidade do oócito em se tornar um embrião saudável é objeto de estudo na medicina reprodutiva humana e v

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Dill, Brian D. "Identification of Chlamydial Iron-Responsive Proteins during Intracellular Growth." Digital Commons @ East Tennessee State University, 2008. https://dc.etsu.edu/etd/1955.

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Chlamydia trachomatis is an obligate intracellular bacterium and the most prevalent cause of bacterial sexually transmitted disease. Genital chlamydial infections, marked by chronic, intense inflammation, can lead to genital tissue scarring and infertility and is a contributing factor to development of pelvic inflammatory disease and ectopic pregnancy. Iron is required as a cofactor for numerous highly conserved pathways, and nearly all studied organisms rely on iron for growth. In response to iron restriction, the chlamydial developmental cycle arrests at the intracellular reticulate body sta

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19

Bittarello, Alis Correia. "Estudo de biomarcadores de mercúrio em peixes da amazônia por meio da metalômica e análise do estresse oxidativo." Botucatu, 2017. http://hdl.handle.net/11449/151174.

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Orientador: Pedro de Magalhães Padilha<br>Resumo: O mercúrio é um metal tóxico, de distribuição ubíqua, com capacidade para bioacumulação e biomagnificação, que provoca alterações em biomoléculas importantes no metabolismo e contribui para o estabelecimento do estresse oxidativo em organismos aquáticos. Logo, o presente estudo teve por objetivo identificar e avaliar possíveis biomarcadores proteicos e/ou enzimáticos da toxicidade do mercúrio em peixes da região amazônica, por meio do estudo metaloproteômico e avaliação do estresse oxidativo. Foram utilizadas metodologias de fracionamento e ide

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20

VILELA, Romel Duarte. "Análise fisiológica e proteômica do meristema apical da cana-de-açúcar (Saccharum spp) sob aplicação de cálcio." Universidade Federal de Pernambuco, 2016. https://repositorio.ufpe.br/handle/123456789/17916.

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Submitted by Natalia de Souza Gonçalves (natalia.goncalves@ufpe.br) on 2016-09-23T12:51:56Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Tese Romel Vilela PPGG-UFPE.pdf: 4096664 bytes, checksum: e8f0113723a098ef7897962a79be3500 (MD5)<br>Made available in DSpace on 2016-09-23T12:51:56Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Tese Romel Vilela PPGG-UFPE.pdf: 4096664 bytes, checksum: e8f0113723a098ef7897962a79be3500 (MD5) Previous issue date: 2016-03-03<br>Na cana-de-açúcar, a c

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SANTIAGU, MODUTHAGUM SOOSAI ADAIKALA INFENTA. "EXPLORING BACTERIAL MOLECULAR FACTORS MODULATING THE SYMBIOTIC INTERACTIONS WITH INSECTS." Doctoral thesis, Università degli Studi di Milano, 2015. http://hdl.handle.net/2434/347400.

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Many of the cellular mechanisms underlying host responses to pathogens have been well conserved during evolution. Homeostatic interactions between insects and commensal microbes are widespread in nature. Commensal microbes have many roles in the biology and lifecycle of most insect species, affecting different aspects of their life. The recently recognized acetic acid bacteria (AAB) are the most abundant microorganisms in the insects and also metabolically linked to one another. These symbionts establish close interactions with their animal host, including insects and mosquitoes. In particular

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22

Poobalane, Saravanane. "Aeromonas hydrophila vaccine development using immunoproteomics." Thesis, University of Stirling, 2007. http://hdl.handle.net/1893/195.

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Aeromonas hydrophila is an opportunistic pathogen that causes a wide range of symptoms and diseases in fish. Development of a commercial vaccine has been problematic due to the heterogenicity between isolates of A. hydrophila. A new approach using immunoproteomics was used in this study to try to develop a vaccine that would protect against a wide range of A. hydrophila strains. The virulence of 14 isolates of A. hydrophila from different geographical regions was determined in common carp (Cyprinus carpio) indicating that 6 isolates were virulent, while 8 isolates were avirulent. Expression of

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23

Brooks, Geoffrey Lance. "Coelomic Fluid Protein Profile in Earthworms Following Bacterial Challenge." Thesis, University of North Texas, 2006. https://digital.library.unt.edu/ark:/67531/metadc5476/.

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Proteomic techniques were used to evaluate the protein profile of the earthworm, (Lumbricus terrestris), following a bacterial challenge. One control group received no injection; a second control group received injections of phosphate buffer solution (PBS). The experimental group received injections of PBS containing (Aeromonas hydrophila). After incubation for 12 hours at 20°C, coelomic fluid was collected from each group for analysis by 2-D electrophoresis. There were significant differences in spot appearance and density between control and experimental groups. Sixteen spots showed a two-

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24

Ngara, Rudo. "A proteomic analysis of drought and salt stress responsive proteins of different sorghum varieties." Thesis, University of the Western Cape, 2009. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_1434_1334579378.

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<p>This study reports on a proteomic analysis of sorghum proteomes in response to salt and hyperosmotic stresses. Two-dimensional gel electrophoresis (2DE) in combination with mass spectrometry (MS) was used to separate, visualise and identify sorghum proteins using both sorghum cell suspension cultures and whole plants. The sorghum cell suspension culture system was used as a source of culture filtrate (CF) proteins. Of the 25 visualised CBB stained CF spots, 15 abundant and well-resolved spots were selected for identification using a combination of MALDI-TOF and MALDI-TOFTOF MS, and database

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Magalhães, Ilídio Miguel Teixeira. "Proteome of biofilm produced by a S. pseudintermedius strain." Master's thesis, Universidade de Aveiro, 2014. http://hdl.handle.net/10773/14291.

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Mestrado em Bioquímica<br>Staphylococcus pseudintermedius is an opportunistic pathogenic bacterium responsible for most skin and post-surgical infections in dogs. The number of bacterial strains resistant to β-lactam antibiotics is increasing and are the major challenges now faced by veterinary medicine. Bacteria that produce biofilm are more resistant to treatment and thus, the production of this structure is already considered a virulence factor. In a biofilm, bacteria are embedded in a matrix of extracellular polymeric substances (EPS) some of which are proteins. With the objective to know

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Malheiros, Jessica Moraes. "Caracterização, quantificação e expressão de proteínas estruturais e regulatórias do tecido muscular esquelético e suas relações com as características de qualidade da carne de bovinos Nelore (Bos indicus)." Universidade Estadual Paulista (UNESP), 2018. http://hdl.handle.net/11449/153865.

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Brosson, Damien. "Analyse protéomique et caractérisation de nouvelles protéines de paroi chez Encephalitozoon cuniculi." Phd thesis, Clermont-Ferrand 2, 2006. http://tel.archives-ouvertes.fr/docs/00/68/86/92/PDF/2006CLF21636.pdf.

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La microsporidie Encephalitozoon cuniculi, parasite intracellulaire obligatoire, pathogène de l'homme, est responsable d'infections opportunistes chez des sujets immunodéprimés. Sa spore est protégée par une épaisse paroi protéo-chitineuse pour laquelle peu de données sur les constituants protéiques sont disponibles. Dans ces travaux, nous avons décrit le protéome exprimé dans les stades tardifs de développement d'E. Cuniculi. Grâce à des extractions protéiques séquentielles et une double stratégie d'analyse protéomique, après électrophorèse et "Shotgun", 177 protéines différentes ont pû être

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28

KOČOVÁ, Pavlína. "Sledování exprese proteinů v savčích buňkách infikovaných virem klíšťové encefalitidy." Master's thesis, 2017. http://www.nusl.cz/ntk/nusl-317333.

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This study is focused on changes in protein expression in a glioblastoma cell line during infection with tick-borne encephalitis virus. Newly synthesized proteins were distinguished from previously synthesized proteins using bioorthogonal chemistry (BONCAT method) to observe changes in protein synthesis. Labelled proteins were visualized using two-dimensional PAGE and western blotting followed by Click reaction on membrane. Differences in protein pattern between control and infected cells were observed.

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LONARDONI, Francesco. "Discovery and quantification of proteins of biological relevance through differential proteomics and biosensing." Doctoral thesis, 2012. http://hdl.handle.net/11562/397149.

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La linea generale su cui si sviluppa il mio progetto di dottorato è la ricerca e misurazione quantitativa di biomarcatori, ed è inserito nel più ampio campo della diagnostica medica. Un biomarcatore è definito in modo chiaro dalla Food and Drug Administration statunitense come “una caratteristica che sia oggettivamente misurabile e valutabile come indicatore di processi fisiologici o patologici o di risposta a trattamento con farmaci”. Il primo progetto di ricerca di questo periodo di dottorato consiste in un’analisi proteomica differenziale su siero di cordone ombelicale e fluido amniotic

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McCabe, Shauna. "Characterization and expression patterns of five Winter Rye ??-1,3-endoglucanases and their role in cold acclimation." Thesis, 2007. http://hdl.handle.net/10012/2689.

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Winter rye produces ice-modifying antifreeze proteins upon cold treatment. Two of these antifreeze proteins are members of the large, highly conserved, ??-1,3-endoglucanase family. This project was designed to identify glucanase genes that are expressed during cold acclimation, wounding, pathogen infection, drought or treatment with the phytohormones ethylene and MeJa. Additionally, a more detailed proteomic analysis was to be carried out to evaluate the glucanase content of the apoplast of cold-acclimated (CA) winter rye. Results of 2D SDS-PAGE analysis revealed that non-acclimated whole le

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Weber, Arnim. "Transkriptom- und Proteom-Analysen von Escherichia coli unter hyperosmotischen Stressbedingungen und biochemische Charakterisierung von UspG." Doctoral thesis, 2003. https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2003112611.

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32

-Ju, Chun, and 陳君茹. "1.Post-translational modification on protein by glyoxal and methylglyoxal2.Characterization contain 3-nitrotyrine protein in human urine by 2D-PAGE and LC/NSI/MS/MS." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/55606096158017229679.

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碩士<br>國立中正大學<br>化學所<br>95<br>1. The non-enzymatic conjugated addition product of glucose or aldehyde derivatives and glycation reaction of protein are the main cause of vascular complications of diabetes. If the concentration of glucose remains at high level in the body, the amount of ?dicarbonyl compounds, such as glyoxal and methylglyoxal, also increases. Glyoxal is obtained from glucose and amino acid oxidation, lipid peroxidation; methylglyoxal is mainly from glucose degradation intermediate product: G-3-P self-decompose. These reactive ?dicarbonyl compounds react with protein, lipids and

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Duchoslav, Miloš. "Proteomická a funkční charakterizace izoforem PsbO." Master's thesis, 2012. http://www.nusl.cz/ntk/nusl-310407.

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PsbO (manganese-stabilizing protein) is the largest extrinsic protein of photosystem II, located on the lumen side of photosystem. It is present in all known oxyphototrophic organisms. PsbO facilitates photosynthetic water splitting, which takes place in an oxygen evolving center (Mn4CaO5 cluster) of photosystem II. This work is focused on PsbO of higher plants and its isoforms, particularly their evolution and functions. Bioinformatic analyses revealed that majority of higher plants express exactly two psbO isoforms. A phylogenetic tree of PsbO sequences has an unusual topology. The two paral

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Rachfall, Nicole. "Translational control by the ribosomal protein Asc1p/Cpc2p in Saccharomyces cerevisiae." Thesis, 2010. http://hdl.handle.net/11858/00-1735-0000-0006-AE00-4.

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