Log in

Relevant bibliographies by topics / 3' untranslated region (3'UTR) / Journal articles

To see the other types of publications on this topic, follow the link: 3' untranslated region (3'UTR).

Journal articles on the topic '3' untranslated region (3'UTR)'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic '3' untranslated region (3'UTR).'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Pan, FengHua, XueXiang Liu, JuFang Huang, and Wei Tian. "Characterization of 3′untranslated region (3′UTR) of the MICB gene." Human Immunology 74, no. 6 (2013): 746–50. http://dx.doi.org/10.1016/j.humimm.2013.01.028.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

López de Silanes, Isabel, María Paz Quesada, and Manel Esteller. "Aberrant Regulation of Messenger RNA 3′-Untranslated Region in Human Cancer." Analytical Cellular Pathology 29, no. 1 (2007): 1–17. http://dx.doi.org/10.1155/2007/586139.

Full text

Abstract:

The messenger RNA 3′-untranslated region (3′UTR) is emerging as critically important in regulating gene expression at posttranscriptional levels. The 3′UTR governs gene expression via orchestrated interactions between mRNA structural components (cis-elements) and specific trans-acting factors (RNA-binding proteins and non-coding RNAs). Alterations in any of these components can lead to disease. Here, we review the mutations in 3′UTR regulatory sequences as well as the aberrant levels, subcellular localization, and posttranslational modifications of trans-acting factors that can promote or enha

APA, Harvard, Vancouver, ISO, and other styles

3

Tanguay, R. L., and D. R. Gallie. "Translational efficiency is regulated by the length of the 3' untranslated region." Molecular and Cellular Biology 16, no. 1 (1996): 146–56. http://dx.doi.org/10.1128/mcb.16.1.146.

Full text

Abstract:

All polyadenylated mRNAs contain sequence of variable length between the coding region and the poly(A) tail. Little has been done to establish what role the length of the 3' untranslated region (3'UTR) plays in posttranscriptional regulation. Using firefly luciferase (luc) reporter mRNA in transiently transfected Chinese hamster ovary (CHO) cells, we observed that the addition of a poly(A) tail increased expression 97-fold when the length of the 3'UTR was 19 bases but that its stimulatory effect was only 2.3-fold when the length of the 3'UTR was increased to 156 bases. The effect of the luc 3'

APA, Harvard, Vancouver, ISO, and other styles

4

Polacek, Charlotta, and A. Michael Lindberg. "Genetic characterization of the coxsackievirus B2 3′ untranslated region." Journal of General Virology 82, no. 6 (2001): 1339–48. http://dx.doi.org/10.1099/0022-1317-82-6-1339.

Full text

Abstract:

The secondary structure of the 3′ untranslated region (3′UTR) of picornaviruses is thought to be important for the initiation of negative-strand RNA synthesis. In this study, genetic and biological analyses of the 3′ terminus of coxsackievirus B2 (CVB2), which differs from other enteroviruses due to the presence of five additional nucleotides prior to the poly(A) tail, is reported. The importance of this extension was investigated using a 3′UTR mutant lacking the five nucleotides prior to the poly(A) tail and containing two point mutations. The predicted secondary structure within the 3′UTR of

APA, Harvard, Vancouver, ISO, and other styles

5

Wang, Wen Yi, Wei Tian, Xue Xiang Liu, and Li Xin Li. "HLA-G coding region and 3′untranslated region (3′UTR) in two Chinese Han populations." Immunology Letters 176 (August 2016): 65–71. http://dx.doi.org/10.1016/j.imlet.2016.05.017.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Cybulsky, Andrey V., Tomoko Takano, Joan Papillon, et al. "The 3′-untranslated region of the Ste20-like kinase SLK regulates SLK expression." American Journal of Physiology-Renal Physiology 292, no. 2 (2007): F845—F852. http://dx.doi.org/10.1152/ajprenal.00234.2006.

Full text

Abstract:

Ste20-like kinase, SLK, a germinal center kinase found in kidney epithelial cells, signals to promote apoptosis. Expression of SLK mRNA and protein and kinase activity are increased during kidney development and recovery from ischemic acute renal failure. The 3′-untranslated region (3′-UTR) of SLK mRNA contains multiple adenine and uridine-rich elements, suggesting that 3′-UTR may regulate mRNA stability. This was confirmed in COS cell transient transfection studies, which showed that expression of the SLK open-reading frame plus 3′-UTR mRNA was reduced by 35% relative to the open-reading fram

APA, Harvard, Vancouver, ISO, and other styles

7

Black, B. L., J. Lu, and E. N. Olson. "The MEF2A 3' untranslated region functions as a cis-acting translational repressor." Molecular and Cellular Biology 17, no. 5 (1997): 2756–63. http://dx.doi.org/10.1128/mcb.17.5.2756.

Full text

Abstract:

Myocyte enhancer factor 2 (MEF2) proteins serve as important muscle transcription factors. In addition, MEF2 proteins have been shown to potentiate the activity of other cell-type-specific transcription factors found in muscle and brain tissue. While transcripts for MEF2 factors are widely expressed in a variety of cells and tissues, MEF2 proteins and binding activity are largely restricted to skeletal, smooth, and cardiac muscle and to brain. This disparity between MEF2 protein and mRNA expression suggests that translational control may play an important role in regulating MEF2 expression. In

APA, Harvard, Vancouver, ISO, and other styles

8

Kim, Hye-Ran, Myung-Geun Shin, Sun-Seog Kweon, et al. "The Prohibitin 3′ Untranslated Region Polymorphisms Are Associated with AML Susceptibility." Blood 114, no. 22 (2009): 5038. http://dx.doi.org/10.1182/blood.v114.22.5038.5038.

Full text

Abstract:

Abstract Abstract 5038 The prohibitin gene, which is located on human chromosome 17q21 encodes a certain regulatory RNA molecule responsible for inhibitory effects and the respective tumor suppressor function. Common C/T and A/G polymorphisms occur at nucleotide 10701 and 10730 respectively in the 3' untranslated region (UTR) of the prohibitin gene. Recent findings indicate that presence of at least one mutant allele within the 3' UTR prohibitin gene polymorphism causes inactivation of bioactive RNA, resulting in loss of its pro-apoptotic function and subsequent risk for malignant growth. Thes

APA, Harvard, Vancouver, ISO, and other styles

9

Newburn, Laura R., Baodong Wu, and K. Andrew White. "Investigation of Novel RNA Elements in the 3′UTR of Tobacco Necrosis Virus-D." Viruses 12, no. 8 (2020): 856. http://dx.doi.org/10.3390/v12080856.

Full text

Abstract:

RNA elements in the untranslated regions of plus-strand RNA viruses can control a variety of viral processes including translation, replication, packaging, and subgenomic mRNA production. The 3′ untranslated region (3′UTR) of Tobacco necrosis virus strain D (TNV-D; genus Betanecrovirus, family Tombusviridae) contains several well studied regulatory RNA elements. Here, we explore a previously unexamined region of the viral 3′UTR, the sequence located upstream of the 3′-cap independent translation enhancer (3′CITE). Our results indicate that (i) a long-range RNA–RNA interaction between an intern

APA, Harvard, Vancouver, ISO, and other styles

10

Leathers, V., R. Tanguay, M. Kobayashi, and D. R. Gallie. "A phylogenetically conserved sequence within viral 3' untranslated RNA pseudoknots regulates translation." Molecular and Cellular Biology 13, no. 9 (1993): 5331–47. http://dx.doi.org/10.1128/mcb.13.9.5331.

Full text

Abstract:

Both the 68-base 5' leader (omega) and the 205-base 3' untranslated region (UTR) of tobacco mosaic virus (TMV) promote efficient translation. A 35-base region within omega is necessary and sufficient for the regulation. Within the 3' UTR, a 52-base region, composed of two RNA pseudoknots, is required for regulation. These pseudoknots are phylogenetically conserved among seven viruses from two different viral groups and one satellite virus. The pseudoknots contained significant conservation at the secondary and tertiary levels and at several positions at the primary sequence level. Mutational a

APA, Harvard, Vancouver, ISO, and other styles

11

Leathers, V., R. Tanguay, M. Kobayashi, and D. R. Gallie. "A phylogenetically conserved sequence within viral 3' untranslated RNA pseudoknots regulates translation." Molecular and Cellular Biology 13, no. 9 (1993): 5331–47. http://dx.doi.org/10.1128/mcb.13.9.5331-5347.1993.

Full text

Abstract:

Both the 68-base 5' leader (omega) and the 205-base 3' untranslated region (UTR) of tobacco mosaic virus (TMV) promote efficient translation. A 35-base region within omega is necessary and sufficient for the regulation. Within the 3' UTR, a 52-base region, composed of two RNA pseudoknots, is required for regulation. These pseudoknots are phylogenetically conserved among seven viruses from two different viral groups and one satellite virus. The pseudoknots contained significant conservation at the secondary and tertiary levels and at several positions at the primary sequence level. Mutational a

APA, Harvard, Vancouver, ISO, and other styles

12

Hennessy, S. W., B. A. Frazier, D. D. Kim, et al. "Complete thrombospondin mRNA sequence includes potential regulatory sites in the 3' untranslated region." Journal of Cell Biology 108, no. 2 (1989): 729–36. http://dx.doi.org/10.1083/jcb.108.2.729.

Full text

Abstract:

The nucleotide sequence of human thrombospondin (TS) mRNA has been determined from human fibroblast and endothelial cDNAs. The sequence of 5802 bp begins 110 bp upstream from the initiator codon and includes the entire 3' untranslated region (UTR) of the mRNA. The coding region (3510 bp) specifies a protein of 1170 amino acids with all of the known features of the TS subunit (Frazier, W. A. 1987. J. Cell Biol. 105:625-632). The long 3' UTR of 2166 nucleotides is extremely A/T-rich, particularly in the latter half. It contains 37 TATT or ATTT(A) sequences that have been suggested as mediators o

APA, Harvard, Vancouver, ISO, and other styles

13

Lee, Daniel Y., Tatiana Shatseva, Zina Jeyapalan, William W. Du, Zhaoqun Deng, and Burton B. Yang. "A 3′-Untranslated Region (3′UTR) Induces Organ Adhesion by Regulating miR-199a* Functions." PLoS ONE 4, no. 2 (2009): e4527. http://dx.doi.org/10.1371/journal.pone.0004527.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Qi, Ting, Yuming Xu, Tong Zhou, and Wanjun Gu. "The Evolution of G-quadruplex Structure in mRNA Untranslated Region." Evolutionary Bioinformatics 17 (January 2021): 117693432110351. http://dx.doi.org/10.1177/11769343211035140.

Full text

Abstract:

The RNA G-quadruplex (rG4) is a kind of non-canonical high-order secondary structure with important biological functions and is enriched in untranslated regions (UTRs) of protein-coding genes. However, how rG4 structures evolve is largely unknown. Here, we systematically investigated the evolution of RNA sequences around UTR rG4 structures in 5 eukaryotic organisms. We found universal selection on UTR sequences, which facilitated rG4 formation in all the organisms that we analyzed. While G-rich sequences were preferred in the rG4 structural region, C-rich sequences were selectively not preferr

APA, Harvard, Vancouver, ISO, and other styles

15

Smith, Spenser S., Catherine B. Kessler, Vikram Shenoy, Clifford J. Rosen, and Anne M. Delany. "IGF-I 3′ Untranslated Region: Strain-Specific Polymorphisms and Motifs Regulating IGF-I in Osteoblasts." Endocrinology 154, no. 1 (2013): 253–62. http://dx.doi.org/10.1210/en.2012-1476.

Full text

Abstract:

Reduced IGF-I is associated with low bone mass in humans and mice. C3H/He/J (C3H) mice have higher skeletal IGF-I and greater bone mass than C57BL/6J (B6). We hypothesized that strain-related genotypic differences in Igf1 affected skeletal function. The Igf1 coding region is nonpolymorphic, but its 3′ untranslated region (UTR) is polymorphic between C3H and B6. Luciferase-Igf1 3′ UTR reporter constructs showed that these polymorphic regions did not affect UTR function. IGF-I splice variants give rise to a common mature IGF-I peptide, but different E peptides. We identified two splice products,

APA, Harvard, Vancouver, ISO, and other styles

16

Shao, Yvonne, та Faramarz Ismail-Beigi. "Control of Na+-K+-ATPase β1-subunit expression: role of 3′-untranslated region". American Journal of Physiology-Cell Physiology 286, № 3 (2004): C580—C585. http://dx.doi.org/10.1152/ajpcell.00117.2003.

Full text

Abstract:

Using in vitro translation and cell transfection assays, we previously demonstrated that the Na+-K+-ATPase β1 mRNA species containing its longest 3′-untranslated region (UTR) exhibited the lowest translational efficiency. Here, employing deletions and in vivo expression assays, using direct injection of plasmids into rat ventricular myocardium, we identified a 143-nt segment located in the distal 3′-UTR of β1 mRNA that was associated with decreased luciferase expression; interestingly, this segment contains three AUUUA motifs. Using RNA-protein binding assays and UV cross-linking of cRNA with

APA, Harvard, Vancouver, ISO, and other styles

17

MARTIGNETTI, LOREDANA, ANDREI ZINOVYEV, and EMMANUEL BARILLOT. "IDENTIFICATION OF SHORTENED 3′ UNTRANSLATED REGIONS FROM EXPRESSION ARRAYS." Journal of Bioinformatics and Computational Biology 10, no. 02 (2012): 1241001. http://dx.doi.org/10.1142/s0219720012410016.

Full text

Abstract:

Cancer cells have been recently shown to express high level of short 3′UTR isoforms that can escape miRNA-mediated regulation. We present here a computational procedure for systematically identifying shortened 3′UTRs by Affymetrix 3′ microarrays. The advantage of this technology compared to more recent and promising ones such as exon arrays and RNA-Seq is that, giving the relatively small cost, already existing datasets in public databases include a considerably higher number of experiments. Moreover, the design of Affymetrix Gene Chips is well-suited for 3′UTR analysis of a large number of ge

APA, Harvard, Vancouver, ISO, and other styles

18

Moseley, P. L., E. S. Wallen, J. D. McCafferty, S. Flanagan, and J. A. Kern. "Heat stress regulates the human 70-kDa heat-shock gene through the 3'-untranslated region." American Journal of Physiology-Lung Cellular and Molecular Physiology 264, no. 6 (1993): L533—L537. http://dx.doi.org/10.1152/ajplung.1993.264.6.l533.

Full text

Abstract:

Cells respond to a variety of stresses by synthesizing a family of proteins termed heat-shock proteins (HSP). Recently, the 3'-untranslated regions (UTRs) of some mRNAs have been shown to be important in the posttranscriptional regulation of protein production. Therefore, we hypothesized that heat could regulate HSP70 production through the HSP70 3'-UTR, in addition to its known effects on transcription. To test this hypothesis, cells were transfected with either a plasmid containing sequences encoding the human HSP70 or beta-globin 3'-untranslated region placed downstream of a chloramphenicol

APA, Harvard, Vancouver, ISO, and other styles

19

Song, Yutong, Peter Friebe, Eleni Tzima, Christiane Jünemann, Ralf Bartenschlager, and Michael Niepmann. "The Hepatitis C Virus RNA 3′-Untranslated Region Strongly Enhances Translation Directed by the Internal Ribosome Entry Site." Journal of Virology 80, no. 23 (2006): 11579–88. http://dx.doi.org/10.1128/jvi.00675-06.

Full text

Abstract:

ABSTRACT The positive-strand RNA genome of the hepatitis C virus (HCV) is flanked by 5′- and 3′-untranslated regions (UTRs). Translation of the viral RNA is directed by the internal ribosome entry site (IRES) in the 5′-UTR, and subsequent viral RNA replication requires sequences in the 3′-UTR and in the 5′-UTR. Addressing previous conflicting reports on a possible function of the 3′-UTR for RNA translation in this study, we found that reporter construct design is an important parameter in experiments testing 3′-UTR function. A translation enhancer function of the HCV 3′-UTR was detected only a

APA, Harvard, Vancouver, ISO, and other styles

20

Wilson, Gerald M., Yue Sun, Jeremy Sellers, et al. "Regulation of AUF1 Expression via Conserved Alternatively Spliced Elements in the 3′ Untranslated Region." Molecular and Cellular Biology 19, no. 6 (1999): 4056–64. http://dx.doi.org/10.1128/mcb.19.6.4056.

Full text

Abstract:

ABSTRACT The A+U-rich RNA-binding factor AUF1 exhibits characteristics of atrans-acting factor contributing to the rapid turnover of many cellular mRNAs. Structural mapping of the AUF1 gene and its transcribed mRNA has revealed alternative splicing events within the 3′ untranslated region (3′-UTR). In K562 erythroleukemia cells, we have identified four alternatively spliced AUF1 3′-UTR variants, including a population of AUF1 mRNA containing a highly conserved 107-nucleotide (nt) 3′-UTR exon (exon 9) and the adjacent downstream intron (intron 9). Functional analyses using luciferase–AUF1 3′-UT

APA, Harvard, Vancouver, ISO, and other styles

21

Romero, Tammy A., Ebenezer Tumban, Jeongwon Jun, William B. Lott, and Kathryn A. Hanley. "Secondary structure of dengue virus type 4 3′ untranslated region: impact of deletion and substitution mutations." Journal of General Virology 87, no. 11 (2006): 3291–96. http://dx.doi.org/10.1099/vir.0.82182-0.

Full text

Abstract:

Several studies have generated computer-based predictions of secondary structure of the 3′ untranslated region (UTR) of Dengue virus (DEN); however, experimental verification of the formation of these structures in vitro is lacking. This study assessed the congruence of Mfold predictions of secondary structure of the core region of the DEN type 4 3′ UTR with nuclease maps of this region. Maps and predictions were largely consistent. Maps supported the existence of previously predicted pseudoknots and identified putative regions of dynamic folding. Additionally, this study investigated previous

APA, Harvard, Vancouver, ISO, and other styles

22

Yocupicio-Monroy, Rosa Martha E., Fernando Medina, Jorge Reyes-del Valle, and Rosa M. del Angel. "Cellular Proteins from Human Monocytes Bind to Dengue 4 Virus Minus-Strand 3′ Untranslated Region RNA." Journal of Virology 77, no. 5 (2003): 3067–76. http://dx.doi.org/10.1128/jvi.77.5.3067-3076.2003.

Full text

Abstract:

ABSTRACT The synthesis of plus and minus RNA strands of several RNA viruses requires as a first step the interaction of some viral regulatory sequences with cellular and viral proteins. The dengue 4 virus genome, a single-stranded, positive-polarity RNA, is flanked by two untranslated regions (UTR) located in the 5′ and 3′ ends. The 3′UTR in the minus-strand RNA [3′UTR (−)] has been thought to function as a promoter for the synthesis of plus-strand RNA. To study the initial interaction between this 3′UTR and cellular and viral proteins, mobility shift assays were performed, and four ribonucleo

APA, Harvard, Vancouver, ISO, and other styles

23

Bittolo, Tamara, Federico Pozzo, Riccardo Bomben, et al. "Mutations at 3' Untranslated Region (3'UTR) of NOTCH1 Are Associated with Low CD20 Expression Levels in Chronic Lymphocytic Leukemia." Blood 128, no. 22 (2016): 306. http://dx.doi.org/10.1182/blood.v128.22.306.306.

Full text

Abstract:

Abstract Background. In chronic lymphocytic leukemia (CLL), NOTCH1 mutations associate with clinical resistance to anti-CD20 immunotherapy in FCR combination (Stilgenbauer et al., Blood, 2014, Dal Bo et al., AHO, 2014), that can be ascribed to a NOTCH1 mutation-driven repression of CD20 levels by HDACs (Pozzo et al., Leukemia, 2016). Recently, novel recurrent mutations have been identified in the 3'untranslated region of NOTCH1 (3'UTR NOTCH1 mutations), determining a novel splicing event within the last NOTCH1 exon (Puente et al., Nature, 2015), leading to an impaired degradation of NOTCH1 pro

APA, Harvard, Vancouver, ISO, and other styles

24

Kawai, Masanobu, Anne M. Delany, Carla B. Green, Martin L. Adamo, and Clifford J. Rosen. "Nocturnin Suppresses Igf1 Expression in Bone by Targeting the 3′ Untranslated Region of Igf1 mRNA." Endocrinology 151, no. 10 (2010): 4861–70. http://dx.doi.org/10.1210/en.2010-0407.

Full text

Abstract:

IGF-I is an anabolic factor that mediates GH and PTH actions in bone. Expression of skeletal Igf1 differs for inbred strains of mice, and Igf expression levels correlate directly with bone mass. Previously we reported that peroxisome proliferator-activated receptor-γ2 activation in bone marrow suppressed Igf1 expression and that peroxisome proliferator-activated receptor-γ2 activation-induced Nocturnin (Noc) expression, a circadian gene with peak expression at light offset, which functions as a deadenylase. In 24-h studies we found that Igf1 mRNA exhibited a circadian rhythm in femur with the

APA, Harvard, Vancouver, ISO, and other styles

25

Samur, Mehmet K., Irtisha Singh, Lee Shih-Han, et al. "3' Untranslated Region (UTR) Alterations Are Frequently Targeted By MM-Related Mirnas and Affects the Clinical Outcome." Blood 128, no. 22 (2016): 4447. http://dx.doi.org/10.1182/blood.v128.22.4447.4447.

Full text

Abstract:

Abstract More than half of human genes use alternative cleavage and polyadenylation to generate alternative 3' untranslated region (3'UTR) isoforms that play a role in gene expression regulation. The 3' untranslated region (3'UTR) of mRNA contains elements that play regulatory roles in polyadenylation, localization, translation efficiency, and mRNA stability. Although relative contributions of different regulatory mechanisms remain poorly understood, several recent studies showed that alterations in 3'UTRs might affect protein localization as well as their interactions with other proteins. Her

APA, Harvard, Vancouver, ISO, and other styles

26

LORENZINI, Erna C., and Immo E. SCHEFFLER. "Co-operation of the 5′ and 3′ untranslated regions of ornithine decarboxylase mRNA and inhibitory role of its 3′ untranslated region in regulating the translational efficiency of hybrid RNA species via cellular factor(s)." Biochemical Journal 326, no. 2 (1997): 361–67. http://dx.doi.org/10.1042/bj3260361.

Full text

Abstract:

The 5′ untranslated region (UTR) has an inhibitory role in the translatability of ornithine decarboxylase (ODC) mRNA and of hybrid mRNA species, whereas the ODC 3′ UTR causes a partial release of this inhibition. We designed experiments to explore whether the co-operation between ODC 5′ UTR and 3′ UTR in the translational regulation is due to a direct interaction of those sequences or whether it is mediated by their interaction with cellular factor(s). We stably transfected Chinese hamster ovary (CHO)-K1 cells and transiently transfected COS-1 cells with expression vectors carrying different c

APA, Harvard, Vancouver, ISO, and other styles

27

LÖVKVIST WALLSTRÖM, Eva, Koichi TAKAO, Anna WENDT, Cristina VARGIU, Hong YIN, and Lo PERSSON. "Importance of the 3′ untranslated region of ornithine decarboxylase mRNA in the translational regulation of the enzyme." Biochemical Journal 356, no. 2 (2001): 627–34. http://dx.doi.org/10.1042/bj3560627.

Full text

Abstract:

Translational regulation of ornithine decarboxylase (ODC), which catalyses the first step in the biosynthesis of polyamines, appears to be an important mechanism in the strong feedback control as well as in the hypotonic induction of the enzyme. However, the exact mechanisms are not yet understood. The ODC mRNA has long 5′ and 3′ untranslated regions (UTRs) which may be involved in the translational control of the enzyme. In the present study we have used a series of stable transfectants of Chinese Hamster ovary cells expressing ODC mRNAs with various truncations in the 5′ and 3′ UTRs to inves

APA, Harvard, Vancouver, ISO, and other styles

28

Ashley, William W., та Brenda Russell. "Tenotomy decreases reporter protein synthesis via the 3′-untranslated region of the β-myosin heavy chain mRNA". American Journal of Physiology-Cell Physiology 279, № 1 (2000): C257—C265. http://dx.doi.org/10.1152/ajpcell.2000.279.1.c257.

Full text

Abstract:

We tested the hypothesis that the β-myosin heavy chain (β-MHC) 3′-untranslated region (UTR) mediates decreased protein expression after tenotomy of the rat soleus. We also tested the hypothesis that decreased protein expression is the result of RNA-protein interactions within the 3′-UTR. β-MHC was chosen for study because of its critical role in the function of postural muscles such as soleus. Adult rat soleus muscles were directly injected with luciferase (LUC) reporter constructs containing either the β-MHC or SV40 3′-UTR. After 48 h of tenotomy, there was no significant effect on LUC expres

APA, Harvard, Vancouver, ISO, and other styles

29

Zhao, Junxing, Jianming Qiu, Sadikshya Aryal, Jennifer L. Hackett, and Jingxin Wang. "The RNA Architecture of the SARS-CoV-2 3′-Untranslated Region." Viruses 12, no. 12 (2020): 1473. http://dx.doi.org/10.3390/v12121473.

Full text

Abstract:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the current COVID-19 pandemic. The 3′ untranslated region (UTR) of this β-CoV contains essential cis-acting RNA elements for the viral genome transcription and replication. These elements include an equilibrium between an extended bulged stem-loop (BSL) and a pseudoknot. The existence of such an equilibrium is supported by reverse genetic studies and phylogenetic covariation analysis and is further proposed as a molecular switch essential for the control of the viral RNA polymerase binding. Here, we report the SARS

APA, Harvard, Vancouver, ISO, and other styles

30

Lowen, Anice C., and Richard M. Elliott. "Mutational Analyses of the Nonconserved Sequences in the Bunyamwera Orthobunyavirus S Segment Untranslated Regions." Journal of Virology 79, no. 20 (2005): 12861–70. http://dx.doi.org/10.1128/jvi.79.20.12861-12870.2005.

Full text

Abstract:

ABSTRACT Bunyamwera virus (BUNV) is the prototype of the genus Orthobunyavirus and the family Bunyaviridae. BUNV has a tripartite genome of negative-sense RNA composed of small (S), medium (M), and large (L) segments. Partially complementary untranslated regions (UTRs) flank the coding region of each segment. The terminal 11 nucleotides of these UTRs are conserved between the three segments and throughout the genus, while the internal regions are unique to each segment and largely nonconserved between different viruses. To investigate the functions of the UTR sequences, we constructed a series

APA, Harvard, Vancouver, ISO, and other styles

31

Thomas, Marc A., Darren M. Preece, and Jacqueline M. Bentel. "Androgen regulation of the prostatic tumour suppressor NKX3.1 is mediated by its 3′ untranslated region." Biochemical Journal 425, no. 3 (2010): 575–83. http://dx.doi.org/10.1042/bj20091109.

Full text

Abstract:

The homeodomain transcription factor NKX3.1 is a prostate-specific tumour suppressor, expression of which is reduced or undetectable in the majority of metastatic prostate tumours. In the normal prostate and in prostate cancer cells, NKX3.1 expression is under tight androgenic control that we have shown to be mediated by its ~2.5 kb 3′UTR (3′ untranslated region). Reporter deletion analysis of the NKX3.1 3′UTR identified three regions that were transactivated by DHT (5α-dihydrotestosterone) in the AR (androgen receptor)-expressing prostate cancer cell line LNCaP. Reversal of DHT effects by the

APA, Harvard, Vancouver, ISO, and other styles

32

Lai, Ching-Fang, Chih-Ying Chen, and Lo-Chun Au. "Comparison between the Repression Potency of siRNA Targeting the Coding Region and the 3′-Untranslated Region of mRNA." BioMed Research International 2013 (2013): 1–5. http://dx.doi.org/10.1155/2013/637850.

Full text

Abstract:

Small interfering RNAs (siRNAs) are applied for post-transcriptional gene silencing by binding target mRNA. A target coding region is usually chosen, although the3′-untranslated region (3′-UTR) can also be a target. This study elucidates whether the coding region or3′-UTR elicits higher repression. pFLuc and pRLuc are two reporter plasmids. A segment ofFLucgene was PCR-amplified and inserted behind the stop codon of theRLucgene of the pRLuc. Similarly, a segment ofRLucgene was inserted behind the stop codon ofFLuc. Two siFLuc and two siRLuc were siRNAs designed to target the central portions o

APA, Harvard, Vancouver, ISO, and other styles

33

Jiang, Yong, Xiang-Sheng Xu та J. Eric Russell. "A Nucleolin-Binding 3′ Untranslated Region Element Stabilizes β-Globin mRNA In Vivo". Molecular and Cellular Biology 26, № 6 (2006): 2419–29. http://dx.doi.org/10.1128/mcb.26.6.2419-2429.2006.

Full text

Abstract:

ABSTRACT The normal expression of human β globin is critically dependent upon the constitutively high stability of its encoding mRNA. Unlike with α-globin mRNA, the specific cis-acting determinants and trans-acting factors that participate in stabilizing β-globin mRNA are poorly described. The current work uses a linker-scanning strategy to identify a previously unknown determinant of mRNA stability within the β-globin 3′ untranslated region (3′UTR). The new determinant is positioned on an mRNA half-stem opposite a pyrimidine-rich sequence targeted by αCP/hnRNP-E, a factor that plays a critica

APA, Harvard, Vancouver, ISO, and other styles

34

Williams, Gwyn D., Ruey-Yi Chang, and David A. Brian. "A Phylogenetically Conserved Hairpin-Type 3′ Untranslated Region Pseudoknot Functions in Coronavirus RNA Replication." Journal of Virology 73, no. 10 (1999): 8349–55. http://dx.doi.org/10.1128/jvi.73.10.8349-8355.1999.

Full text

Abstract:

ABSTRACT Secondary and tertiary structures in the 3′ untranslated region (UTR) of plus-strand RNA viruses have been postulated to function as control elements in RNA replication, transcription, and translation. Here we describe a 54-nucleotide (nt) hairpin-type pseudoknot within the 288-nt 3′ UTR of the bovine coronavirus genome and show by mutational analysis of both stems that the pseudoknotted structure is required for the replication of a defective interfering RNA genome. The pseudoknot is phylogenetically conserved among coronaviruses both in location and in shape but only partially in nu

APA, Harvard, Vancouver, ISO, and other styles

35

THEKKUMKARA, Thomas J., and Stuart L. LINAS. "Evidence for involvement of 3'-untranslated region in determining angiotensin II receptor coupling specificity to G-protein." Biochemical Journal 370, no. 2 (2003): 631–39. http://dx.doi.org/10.1042/bj20020960.

Full text

Abstract:

The mRNA 3′-untranslated region (3′-UTR) of many genes has been identified as an important regulator of the mRNA transcript itself as well as the translated product. Previously, we demonstrated that Chinese-hamster ovary-K1 cells stably expressing angiotensin receptor subtypes (AT1A) with and without 3′-UTR differed in AT1A mRNA content and its coupling with intracellular signalling pathways. Moreover, RNA mobility-shift assay and UV cross-linking studies using the AT1A 3′-UTR probe identified a major mRNA-binding protein complex of 55kDa in Chinese-hamster ovary-K1 cells. In the present study

APA, Harvard, Vancouver, ISO, and other styles

36

Zhou, G. L., Y. Cao, Y. Z. Xin, Y. F. Song, and H. G. Jin. "Alternative polyadenylation and polymorphisms of 3'untranslated regions of bovine BBOX1 gene." Czech Journal of Animal Science 63, No. 5 (2018): 188–94. http://dx.doi.org/10.17221/105/2016-cjas.

Full text

Abstract:

l-Carnitine, a key element in fatty acid metabolism and energy production, is biosynthesized from gamma-butyro-betaine by the catalysis of gamma-butyrobetaine hydroxylase (BBOX1). We cloned three different 3'untranslated regions (3'UTRs) alternative polyadenylation (APA) transcripts of the BBOX1 gene with different 3'UTR length (GenBank Accession Nos. KX431577, KX431578, KX431579). Two polymorphisms, NM_001101881.2: g.1797_1798insTGC and g.1935T&gt;C, were revealed in 3'UTR of BBOX1 gene. They created or disrupted a restriction site for endonuclease BbvI and HincII, respectively. Moreover,

APA, Harvard, Vancouver, ISO, and other styles

37

Goebel, Scott J., Jill Taylor, and Paul S. Masters. "The 3′ cis-Acting Genomic Replication Element of the Severe Acute Respiratory Syndrome Coronavirus Can Function in the Murine Coronavirus Genome." Journal of Virology 78, no. 14 (2004): 7846–51. http://dx.doi.org/10.1128/jvi.78.14.7846-7851.2004.

Full text

Abstract:

ABSTRACT The 3′ untranslated region (3′ UTR) of the genome of the severe acute respiratory syndrome coronavirus can functionally replace its counterpart in the prototype group 2 coronavirus mouse hepatitis virus (MHV). By contrast, the 3′ UTRs of representative group 1 or group 3 coronaviruses cannot operate as substitutes for the MHV 3′ UTR.

APA, Harvard, Vancouver, ISO, and other styles

38

Lucena-Silva, Norma, Veridiana Sales Barbosa de Souza, Renan Garcia Gomes, et al. "HLA-G 3′ Untranslated Region Polymorphisms Are Associated with Systemic Lupus Erythematosus in 2 Brazilian Populations." Journal of Rheumatology 40, no. 7 (2013): 1104–13. http://dx.doi.org/10.3899/jrheum.120814.

Full text

Abstract:

Objective.HLA-G has well recognized tolerogenic properties in physiological and nonphysiological conditions. The 3′ untranslated region (3′UTR) of theHLA-Ggene has at least 3 polymorphic sites (14-bpINS/DEL, +3142C/G, and +3196C/G) described as associated with posttranscriptional influence on messenger RNA production; however, only the 14-bpINS/DEL and +3142C/G sites have been studied in systemic lupus erythematosus (SLE).Methods.We investigated theHLA-G3′UTR polymorphic sites (14-bpINS/DEL, +3003C/T, +3010C/G, +3027A/C, +3035C/T, +3142C/G, +3187A/G, and +3196C/G) in 190 Brazilian patients wit

APA, Harvard, Vancouver, ISO, and other styles

39

Nanbu, R., P. A. Menoud, and Y. Nagamine. "Multiple instability-regulating sites in the 3' untranslated region of the urokinase-type plasminogen activator mRNA." Molecular and Cellular Biology 14, no. 7 (1994): 4920–28. http://dx.doi.org/10.1128/mcb.14.7.4920.

Full text

Abstract:

In LLC-PK1 cells urokinase-type plasminogen activator (uPA) mRNA has a short half-life. It is stabilized by inhibition of protein synthesis and by downregulation of protein kinase C (PKC). In the present study on uPA mRNA metabolism, we focused our attention on the 3' untranslated region (3'UTR) of the uPA mRNA, as this region is long and highly conserved among several mammalian species, including mice and humans. To investigate the possible role of the 3'UTR of uPA mRNA in mRNA metabolism, we inserted this region into the 3'UTR of the rabbit beta-globin gene that is linked to the cytomegalovi

APA, Harvard, Vancouver, ISO, and other styles

40

Nanbu, R., P. A. Menoud, and Y. Nagamine. "Multiple instability-regulating sites in the 3' untranslated region of the urokinase-type plasminogen activator mRNA." Molecular and Cellular Biology 14, no. 7 (1994): 4920–28. http://dx.doi.org/10.1128/mcb.14.7.4920-4928.1994.

Full text

Abstract:

In LLC-PK1 cells urokinase-type plasminogen activator (uPA) mRNA has a short half-life. It is stabilized by inhibition of protein synthesis and by downregulation of protein kinase C (PKC). In the present study on uPA mRNA metabolism, we focused our attention on the 3' untranslated region (3'UTR) of the uPA mRNA, as this region is long and highly conserved among several mammalian species, including mice and humans. To investigate the possible role of the 3'UTR of uPA mRNA in mRNA metabolism, we inserted this region into the 3'UTR of the rabbit beta-globin gene that is linked to the cytomegalovi

APA, Harvard, Vancouver, ISO, and other styles

41

Serrano, Paula, Miguel Rodriguez Pulido, Margarita Sáiz, and Encarnacion Martínez-Salas. "The 3′ end of the foot-and-mouth disease virus genome establishes two distinct long-range RNA–RNA interactions with the 5′ end region." Journal of General Virology 87, no. 10 (2006): 3013–22. http://dx.doi.org/10.1099/vir.0.82059-0.

Full text

Abstract:

The untranslated regions (UTRs) of the foot-and-mouth disease virus (FMDV) genome contain multiple functional elements. In the 5′ UTR, the internal ribosome entry site (IRES) element governs cap-independent translation initiation, whereas the S region is presumably involved in RNA replication. The 3′ UTR, composed of two stem–loops and a poly(A) tract, is required for viral infectivity and stimulates IRES activity. Here, it was found that the 3′ end established two distinct strand-specific, long-range RNA–RNA interactions, one with the S region and another with the IRES element. These interact

APA, Harvard, Vancouver, ISO, and other styles

42

Gritsun, T. S., and E. A. Gould. "The 3′ untranslated regions of Kamiti River virus and Cell fusing agent virus originated by self-duplication." Journal of General Virology 87, no. 9 (2006): 2615–19. http://dx.doi.org/10.1099/vir.0.81950-0.

Full text

Abstract:

Previously, it was shown that the 3′ untranslated region (3′UTR) of Kamiti River virus (KRV) is nearly twice as long as the 3′UTR of other flaviviruses (1208 nucleotides compared with 730 nucleotides for the longest 3′UTR of any virus in the Tick-borne encephalitis virus species). Additionally, KRV and the closely related Cell fusing agent virus (CFAV) were shown to contain two short, almost perfect repeat sequences of 67 nucleotides. However, the construction of a robust comparative nucleotide alignment has now revealed that the double-length 3′UTR and the direct repeats resulted from the vir

APA, Harvard, Vancouver, ISO, and other styles

43

Mutsuro-Aoki, Hiromi, Hiroshi Teramura, Ryoko Tamukai, et al. "Dissection of a rice OsMac1 mRNA 5’ UTR to uncover regulatory elements that are responsible for its efficient translation." PLOS ONE 16, no. 7 (2021): e0253488. http://dx.doi.org/10.1371/journal.pone.0253488.

Full text

Abstract:

The untranslated regions (UTRs) of mRNAs are involved in many posttranscriptional regulatory pathways. The rice OsMac1 mRNA has three splicing variants of the 5’ UTR (UTRa, UTRb, and UTRc), which include a CU-rich region and three upstream open reading frames (uORFs). UTRc contains an additional 38-nt sequence, termed sp38, which acts as a strong translational enhancer of the downstream ORF; reporter analysis revealed translational efficiencies >15-fold higher with UTRc than with the other splice variants. Mutation analysis of UTRc demonstrated that an optimal sequence length of sp38, rathe

APA, Harvard, Vancouver, ISO, and other styles

44

LIEGRO, Carlo M. Di, Marianna BELLAFIORE, José M. IZQUIERDO, Anja RANTANEN, and José M. CUEZVA. "3′-Untranslated regions of oxidative phosphorylation mRNAs function in vivo as enhancers of translation." Biochemical Journal 352, no. 1 (2000): 109–15. http://dx.doi.org/10.1042/bj3520109.

Full text

Abstract:

Recent findings have indicated that the 3´-untranslated region (3´-UTR) of the mRNA encoding the β-catalytic subunit of the mitochondrial H+-ATP synthase has an in vitro translation-enhancing activity (TEA) [Izquierdo and Cuezva, Mol. Cell. Biol. (1997) 17, 5255–5268; Izquierdo and Cuezva, Biochem. J. (2000) 346, 849–855]. In the present work, we have expressed chimaeric plasmids that encode mRNA variants of green fluorescent protein in normal rat kidney and liver clone 9 cells to determine whether the 3´-UTRs of nuclear-encoded mRNAs involved in the biogenesis of mitochondria have an intrinsi

APA, Harvard, Vancouver, ISO, and other styles

45

Marzbany, Marzieh, and Mahsa Rasekhian. "Effect of encephalomyocarditis virus 3'Untranslated region on GFP transient expression: implications in recombinant protein production." Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas 19, no. 6 (2020): 542–54. http://dx.doi.org/10.37360/blacpma.20.19.6.38.

Full text

Abstract:

The enrichment of therapeutic protein production yield in mammalian cell cultures by modulating mRNA stability is a fairly new strategy in biotechnological applications. Here, we describe the application of 3′-untranslated region (3′UTR) from RNA viral genome to modulate mRNA stability. The data obtained showed that the use of the 3 'UTR sequence of the encephalomyocarditis virus (EMCV 3'UTR) downstream of the target gene was not able to significantly modulate the free energy density indicators of the RNA. However, the sequence influenced the stability of the mRNA (and, therefore, the amount o

APA, Harvard, Vancouver, ISO, and other styles

46

Blum, Juliana L., Allen M. Samarel, and Ruben Mestril. "Phosphorylation and binding of AUF1 to the 3′-untranslated region of cardiomyocyte SERCA2a mRNA." American Journal of Physiology-Heart and Circulatory Physiology 289, no. 6 (2005): H2543—H2550. http://dx.doi.org/10.1152/ajpheart.00545.2005.

Full text

Abstract:

Experimental animals and patients with cardiac hypertrophy and heart failure display abnormally slowed myocardial relaxation, which is associated with downregulation of sarco(endo)plasmic reticulum calcium ATPase 2a (SERCA2a), the cardiomyocyte sarcoplasmic reticulum Ca2+ pump. We previously showed that SERCA2a downregulation can be simulated in cultured neonatal rat ventricular myocytes (NRVM) by treatment with the hypertrophic agonist phorbol myristate acetate (PMA) or by overexpression of the novel protein kinase C (PKC) isoenzymes PKCδ and PKCε. PKC activation, in turn, decreased SERCA2a p

APA, Harvard, Vancouver, ISO, and other styles

47

Yang, Weining, and Albert J. M. Yee. "Versican 3′-untranslated region (3′UTR) promotes dermal wound repair and fibroblast migration by regulating miRNA activity." Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1843, no. 7 (2014): 1373–85. http://dx.doi.org/10.1016/j.bbamcr.2014.02.015.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Matsushita, Shoko, Kentaro Suzuki, Yukiko Ogino, et al. "Androgen Regulates Mafb Expression Through its 3′UTR During Mouse Urethral Masculinization." Endocrinology 157, no. 2 (2015): 844–57. http://dx.doi.org/10.1210/en.2015-1586.

Full text

Abstract:

Abstract External genitalia are prominent organs showing hormone-dependent sexual differentiation. Androgen is an essential regulator of masculinization of the genital tubercle, which is the anlage of external genitalia. We have previously shown that v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B (MAFB) is an androgen-inducible regulator of embryonic urethral masculinization in mice. However, it remains unclear how androgen regulates Mafb expression. The current study suggests that the Mafb 3′ untranslated region (UTR) is an essential region for its regulation by androgen. We i

APA, Harvard, Vancouver, ISO, and other styles

49

NURY, David, Hervé CHABANON, Marilyne LEVADOUX-MARTIN, and John HESKETH. "An eleven nucleotide section of the 3′-untranslated region is required for perinuclear localization of rat metallothionein-1 mRNA." Biochemical Journal 387, no. 2 (2005): 419–28. http://dx.doi.org/10.1042/bj20040630.

Full text

Abstract:

Localization of mRNAs provides a novel mechanism for synthesis of proteins close to their site of function. MT1 (metallothionein-1) is a small, metal-binding protein that is largely cytoplasmic but which can be found in the nucleus. The localization of rat MT1 requires the perinuclear localization of its mRNA by a mechanism dependent on the 3′-UTR (3′-untranslated region). The present study investigates the nature of this mRNA localization signal using Chinese-hamster ovary cells transfected with gene constructs in which either MT1 or the globin coding region is linked to different sequences f

APA, Harvard, Vancouver, ISO, and other styles

50

Meredith, Janet M., Jonathan B. Rohll, Jeffrey W. Almond, and David J. Evans. "Similar Interactions of the Poliovirus and Rhinovirus 3D Polymerases with the 3′ Untranslated Region of Rhinovirus 14." Journal of Virology 73, no. 12 (1999): 9952–58. http://dx.doi.org/10.1128/jvi.73.12.9952-9958.1999.

Full text

Abstract:

ABSTRACT We showed previously that a human rhinovirus 14 (HRV14) 3′ untranslated region (3′ UTR) on a poliovirus genome was able to replicate with nearly wild-type kinetics (J. B. Rohll, D. H. Moon, D. J. Evans, and J. W. Almond, J. Virol 69:7835–7844, 1995). This enabled the HRV14 single 3′ UTR stem-loop structure to be studied in combination with a sensitive reporter system, poliovirus FLC/REP, in which the capsid coding region is replaced by an in-frame chloramphemicol acetyltransferase (CAT) gene. Using such a construct, we identified a mutant (designated mut4), in which the structure and

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!