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Journal articles on the topic '342.25: 35.076.12(043.2)'

Author: Grafiati

Published: 30 May 2022

Last updated: 31 July 2025

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1

Li, Bing, Jinqin Liu, Shiqiang Qu, et al. "Colony-Forming Unit Cell (CFU-C) Assays in Myelodysplastic Syndrome." Blood 126, no. 23 (2015): 2874. http://dx.doi.org/10.1182/blood.v126.23.2874.2874.

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Abstract Introduction: The myelodysplastic syndromes (MDS) are a group of clonal diseases derived from hematopoietic stem cells (HSC). Colony-forming unit cell (CFU-C) assay is an effective method to study the number and the function of HSC in vitro. In this study, we focus on the characteristics and the prognostic value of CFU-C in patients with MDS. Patients and Method: CFU-C assays were performed according to the protocol of MethoCultTM H4435 Enriched (STEMCELL Technologies). A colony was defined as an aggregate of >40 cells. Clusters consisted of 4 to 40 cells. 560 consecutive newly-dia

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Abu-Khalaf, Maysa, Fnu Nikita, Ayako Shimada, et al. "Abstract P4-11-32: Change in body mass index in breast cancer survivors." Cancer Research 82, no. 4_Supplement (2022): P4–11–32—P4–11–32. http://dx.doi.org/10.1158/1538-7445.sabcs21-p4-11-32.

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Abstract Background: Obesity is associated with an increased risk of breast cancer recurrence and poor survival. Obesity rate in adults in the city of Philadelphia is high, with non-Hispanic blacks and Hispanics having the highest rates. We sought to evaluate changes in body mass index (BMI) in breast cancer survivors within the first 2 years from initial encounter for a breast cancer (BC) diagnosis (dx), and investigate factors that may correlate with a change in BMI. Methods: We identified 5,423 BC patients (pts) in our electronic medical record, (1/2015-present), using ICD-10 code C50.X. We

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Oran, Betul, Richard E. Champlin, Jorge E. Cortes, et al. "Allogeneic Hematopoietic Stem Cell Transplantation (HCT) in First Remission Improves Outcome Irrespective of FLT3-ITD Allelic Burden Among Patients with Acute Myeloid Leukemia and FLT3-ITD Mutation." Blood 124, no. 21 (2014): 2531. http://dx.doi.org/10.1182/blood.v124.21.2531.2531.

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Abstract Presence of internal tandem duplication in the FMS-like tyrosine kinase 3 gene(s) (FLT3-ITD) is associated with poor outcome among patients with cytogenetically normal acute myelogenous leukemia (CN-AML). Patients carrying a higher FLT3-ITD allelic burden have worse outcome. Allogeneic stem cell transplantation (SCT) in first remission (CR1) is reported to improve survival in this poor risk patients. We investigated the impact of FLT3-ITD allelic burden, number of FLT3-ITD mutations and SCT with a matched related or unrelated donor in CR1 in newly diagnosed FLT3-ITD mutated intermedia

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Glueck, C. J., H. I. Glueck, L. Mieczkowski, T. Tracy, J. Speirs, and D. Stroop. "Familial High Plasminogen Activator Inhibitor with Hypofibrinolysis, a New Pathophysiologic Cause of Osteonecrosis?" Thrombosis and Haemostasis 69, no. 05 (1993): 460–65. http://dx.doi.org/10.1055/s-0038-1651633.

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SummaryIn a 29 year old white male with osteonecrosis of both hips and a shoulder, and in his family, we measured basal and stimulated (10 min cuff venous occlusion at 100 mgHg) fibrinolytic activity to determine whether low fibrinolytic activity might be heritable and etiologically associated with osteonecrosis. The proband’s basal tPA-Fx was low, 0.08 IU/ml (normal 0.11-1.94), tPA-Ag was normal (11.6 ng/ml), plasminogen activator inhibitor activity (PAI-Fx) was very high, 119 U/ml (normal 3.5-27), as was his plasminogen activator inhibitor antigen (PAI-Ag), 202 ng/ml (normal 3.2-37.1). The p

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Rabaglio, Manuela, Daniel Dietrich, Bernhard Scheibe, et al. "Abstract P4-01-25: Safety analysis after 11 years of follow-up of the randomized phase III trial SAKK22/99: upfront chemotherapy in advanced HER2 positive breast cancer." Cancer Research 83, no. 5_Supplement (2023): P4–01–25—P4–01–25. http://dx.doi.org/10.1158/1538-7445.sabcs22-p4-01-25.

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Abstract Background: The SAKK 22/99 is a phase III randomized clinical trial launched by the Swiss Group for Clinical Cancer Research and the European Institute of Oncology in Milan in 99 for women with HER2-positive advanced breast cancer (ABC). 175 patients were randomized 1:1 from Sept 99 to Jan 2013 to receive first-line trastuzumab (T) alone followed at disease progression by the combination with chemo (Arm A) vs the upfront combination of T and chemo (Arm B). The results were published in 2017 (O. Pagani et al Ann Onc 28: 305–312, 2017). The outcome was similar for sequential T-chemo or

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Fahey, Margaret C., Marion E. Hare, Gerald W. Talcott, et al. "Characteristics Associated With Participation in a Behavioral Weight Loss Randomized Control Trial in the U.S. Military." Military Medicine 184, no. 3-4 (2018): e120-e126. http://dx.doi.org/10.1093/milmed/usy199.

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Abstract Introduction Effective recruitment and subsequent enrollment of diverse populations is often a challenge in randomized controlled trials, especially those focused on weight loss. In the civilian literature, individuals identified as racial and ethnic minorities, men, and younger and older adults are poorly represented in weight loss interventions. There are limited weight loss trials within military populations, and to our knowledge, none reported participant characteristics associated with enrollment. There may be unique motives and barriers for active duty personnel for enrollment i

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Sasaki, Koji, Ildefonso Ismael Rodriguez-Rivera, Hagop M. Kantarjian, et al. "Correlation of Lymphocyte Count with Treatment Response to Tyrosine Kinase Inhibitors in Newly Diagnosed Chronic Myeloid Leukemia in Chronic Phase." Blood 124, no. 21 (2014): 4538. http://dx.doi.org/10.1182/blood.v124.21.4538.4538.

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Abstract Background: Total lymphocyte count (TLC) has been shown to correlate with outcomes in patients (pts) with acute leukemia. The clinical correlation to TLC in pts with chronic myeloid leukemia in chronic phase (CML-CP) who were treated with a tyrosine-kinase inhibitor (TKI) is unclear. Methods: Lymphocyte data in pts with newly diagnosed CML-CP who were enrolled in consecutive or parallel clinical trials with front-line imatinib (IM), nilotinib (Nilo), or dasatinib (Dasa) were collected at the time of diagnosis, and 3 and 6 months (M) after the start of TKI. Relative lymphocytrosis (RLC

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Arora, Sankalp, Musa Yilmaz, Wei-Ying Jen, et al. "Characteristics and Outcomes of Patients (pts) with Acute Myeloid Leukemia (AML) and FLT3-Tyrosine Kinase Domain (TKD) Mutations." Blood 144, Supplement 1 (2024): 841. https://doi.org/10.1182/blood-2024-204042.

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Background: FLT3-TKD mutations are present in 7-10% of newly diagnosed AML. However, its prognostic impact remains poorly defined, especially in the current treatment landscape, wherein FLT3-inhibitors (FLT3i) and venetoclax (VEN) are often used in frontline regimens. In one of the largest contemporary series, we describe the characteristics and outcomes of frontline FLT3-TKD AML, including impact of frontline treatment and allogeneic stem cell transplant (allo SCT). Methods: Retrospective analysis including all pts with AML who received frontline AML therapy at MDACC, Houston from 01/2012 - 1

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Van Weelderen, Romy E., Kim Klein, Bianca F. Goemans, et al. "Outcome of (Novel) Subgroups in 1257 Pediatric Patients with KMT2A-Rearranged Acute Myeloid Leukemia (AML) and the Significance of Minimal Residual Disease (MRD) Status: A Retrospective Study By the I-BFM-SG." Blood 136, Supplement 1 (2020): 26–27. http://dx.doi.org/10.1182/blood-2020-136064.

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Introduction Outcome of KMT2A-rearranged (KMT2A-r) pediatric AML (pAML) is in general poor with a 5-year probability of event-free survival (5y-pEFS) and overall survival (5y-pOS) of 44% and 56%, respectively (Balgobind et al., 2009). However, over the past decades, the heterogeneity of KMT2A-r pAML has emerged, showing differences in outcome between subgroups based on translocation partners. The predictive value of MRD in KMT2A-r pAML is undefined. This retrospective study aimed to confirm the outcome of pediatric KMT2A subgroups (Balgobind et al., 2009) in a more recent era and to study the

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Vachhani, Pankit, Jennifer Repp, JE Hamer-Maansson, Evan M. Braunstein, Valkal Bhatt, and Haifa Kathrin Al-Ali. "Clinical Outcomes in Patients with Myelofibrosis Treated with Ruxolitinib and Anemia-Supporting Medications." Blood 144, Supplement 1 (2024): 4546. https://doi.org/10.1182/blood-2024-203924.

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Introduction: Patients with myelofibrosis (MF) often present with anemia, and dose-dependent anemia is a known consequence of treatment with the Janus kinase (JAK)1/JAK2 inhibitor ruxolitinib. Current guidelines recommend erythropoiesis-stimulating agents (ESAs) and danazol as options to manage anemia in this population. However, data are lacking regarding the clinical outcomes of patients with MF treated with ruxolitinib and these agents. This post hoc analysis of the phase 3 JUMP trial, the largest ruxolitinib trial to date, evaluated treatment patterns and clinical outcomes of ruxolitinib-t

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Prin Felix, Laura, Maxime Jullien, Amandine Le Bourgeois, et al. "Treosulfan Vs Busulfan As Part of Clofarabine Based Reduced Intensity Conditioning Regimen before Allotransplant for Myeloid Malignancies." Blood 144, Supplement 1 (2024): 4882. https://doi.org/10.1182/blood-2024-203266.

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Introduction: Clofarabine-busulfan based reduced intensity conditioning (RIC) regimens have been proved to be efficient in providing good survivals in adults allotransplanted for myeloid malignancies. Recently, treosulfan replacing busulfan as part of a fludarabine/busulfan RIC regimen was shown to provide better survival thanks to a lower non relapse mortality in older or comorbid allotransplanted patients with acute myeloid leukaemia (AML) or myelodysplastic syndrome (Beelen, Lancet Haematology 2019). Here we provide the results of allotransplant after using a clofarabine/treosulfan RIC regi

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Bringhen, Sara, Alessandra Larocca, Davide Rossi, et al. "Efficacy and Safety of Once Weekly Bortezomib In Multiple Myeloma Patients." Blood 116, no. 21 (2010): 3029. http://dx.doi.org/10.1182/blood.v116.21.3029.3029.

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Abstract Abstract 3029 Background. In a recent phase 3 trial, bortezomib–melphalan – prednisone–thalidomide followed by maintenance treatment with bortezomib–thalidomide (VMPT-VT) demonstrated superior efficacy compared with VMP. Peripheral neuropathy (PN) was the most important dose limiting toxicity. To decrease neurologic toxicities, the protocol was amended and patients in both arms received once-weekly instead of the initial twice-weekly bortezomib infusions. This post-hoc analysis assessed the impact of bortezomib dose-modification schedule on clinical outcomes and safety. Methods. Patie

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M., N. Abubacker, and Mehala T. "Physico-chemical analysis of textile dye effluent using microbial consortia mediated degradation process." Biolife 5, no. 1 (2022): 60–67. https://doi.org/10.5281/zenodo.7359415.

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<strong>ABSTRACT</strong> Physico-chemical analysis of textile dye effluent have shown higher concentrations of total solids, total suspended solids, total dissolved solids, biological oxygen demand, chemical oxygen demand, dissolved oxygen, total hardness, carbonate, bicarbonate alkalinities, chloride, calcium, magnesium, sodium, sulphate, zinc, chromium, copper and lead was found to be above permissible levels of WHO standards which ensure the presence of pollutant overloaded in the textile effluent. Bacterial consortia was developed with <em>Bacillus subtilis </em>(NCBT 012), <em>Clostridiu

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Bashonga, Bishobibiri Alexis, Sande Eric, Ntakimazi Gaspard, and Kahindo Charles. "Bird Ecology in the Ruzizi Delta, Northern End of Lake Tanganyika in Burundi and in the Democratic Republic of Congo." Biolife 11, no. 1 (2023): 12–20. https://doi.org/10.5281/zenodo.7686228.

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<strong>Abstract</strong> Bird ecology in the Ruzizi Delta was investigated during the months of April, July and October 2019-2021. Three species of water birds were the most prominent, the Cattle Egret (Ardeidae), Bubulcus ibis (Linnaeus, 1758) «Héron garde boeufs», the Black Crake (Rallidae) Amaurornis flavirostris (Swainson, 1837) «Râle à bec jaune» and the Africana Jacana (Jacanidae) Actophilornis africanus (Gmelin, 1789) «Jacana a poitrine dorée». The objective pursued by the research is the sustainable conservation of birds b

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Du, Mengxi, and Fang Fang Zhang. "Dietary Supplement Use Among Adult Cancer Survivors in the United States." FASEB Journal 31, S1 (2017). http://dx.doi.org/10.1096/fasebj.31.1_supplement.168.6.

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BackgroundMany cancer patients initiated supplement use after cancer diagnosis and yet the effect of supplement use on survival has not been established. The trend of and factors associated with dietary supplement use in cancer survivors warrant further investigation.MethodsWe examined the trend of dietary supplemental use in 1,902 adult cancer survivors who participated in the National Health and Nutrition Examination Survey (NHANES) from 1999 to 2012, and compared the frequency of supplement use in cancer survivors to that of 3,804 individuals with no history of cancer who were matched to th

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McLeod, Charlie, Michael Dymock, Katie Flanagan, et al. "P-98. The Platform Trial In COVID-19 priming and BOOsting (PICOBOO): the immunogenicity, reactogenicity, and safety of different COVID-19 vaccinations administered as a second booster (fourth dose) in BNT162b2 primed individuals aged 18-< 50 and 50-< 70 years old." Open Forum Infectious Diseases 12, Supplement_1 (2025). https://doi.org/10.1093/ofid/ofae631.305.

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Abstract Background PICOBOO is a randomised, adaptive trial evaluating the immunogenicity, reactogenicity, and safety of COVID-19 booster strategies. Here, we report data for second boosters among individuals aged 18-&lt; 50 and 50-&lt; 70 years old primed with BNT162b2 (18-&lt; 50y-BNT162b2 and 50-&lt; 70y-BNT162b2, respectively) until Day (D) 84. CONSORT diagram for participants recruited to the 18-&lt;50y-BNT162b2 (A) and 50-&lt;70y-BNT162b2 (B) strata for second booster vaccines. Participants were excluded from subsequent analyses if they were infected with COVID-19

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sprotocols. "Synthesis of nNOS-Capon interaction inhibitors: ZLc-002 and its derivatives." December 31, 2014. https://doi.org/10.5281/zenodo.13641.

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Authors: Ting-You Li, Lei Chang, Yu Ma & Dong-Ya Zhu ### Abstract nNOS-Capon interaction is involved in anxiety disorder, disrupting the nNOS-Capon interaction has been demonstrated to show anxiolytic-like effect. In this protocol, N-(2-carbomethoxyacetyl)-D-valine methyl ester, a nNOS-Capon interaction inhibitor, we named it ZLc-002, was prepared by condensation of D-valine methyl ester hydrochloride with methyl malonyl chloride. The synthesis of some analogues of ZLc-002 was also described. ### Introduction Anxiety disorders are highly prevalent psychiatric diseases (1). Selective seroto

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sprotocols. "Preparation of materials for flexizyme reactions and genetic code reprogramming." January 24, 2015. https://doi.org/10.5281/zenodo.14305.

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Authors: Yuki Goto, Takayuki Katoh & Hiroaki Suga ### Abstract Genetic code reprogramming is a method for the reassignment of arbitrary codons from proteinogenic amino acids to non-proteinogenic ones, and thus specific sequences of non-standard peptides can be ribosomally expressed according to their mRNA templates. We have developed a protocol that facilitates the genetic code reprogramming using flexizymes integrated with a custom-made in-vitro translation apparatus, referred to as the flexible in-vitro translation (FIT) system. Flexizymes are flexible tRNA acylation ribozymes that enabl

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Dana O. Kareem, Ahmed MS. Amin, Ayman M. Mustafa, et al. "Tracing Hematological Shifts in Pregnancy: How Anemia and Thrombocytopenia Evolve Across Trimesters." Barw Medical Journal 3, no. 2 (2025). https://doi.org/10.58742/bmj.v3i2.171.

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Abstract Introduction Given pregnancy's significant impact on hematological parameters, monitoring these changes across trimesters is crucial. This study aims to evaluate hematological profiles in pregnant women, primarily focusing on the prevalence of anemia and thrombocytopenia throughout the different trimesters. Methods This retrospective cross-sectional study was conducted at Smart Health Tower from March to December 2024, with ethical approval from Kscien Organization. Pregnant women aged 18-45 years in any trimester were included, excluding those with pre-existing hematological disorder

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sprotocols. "A simple improvement of the conventional cryopreservation for human ES and iPS cells." December 31, 2014. https://doi.org/10.5281/zenodo.13659.

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Authors: Midori Ozawa, Yutaka Ozawa, Masashi Iemura, Arihiro Kohara, Kana Yanagihara & Miho K Furue ### Abstract In this study, a simple method for the cryopreservation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells is proposed. It is based on the conventional slow-freezing method with 10% DMSO and modified mainly in a thawing protocol without specific equipment or reagents. Recovery rate of the cells cryopreserved by this method was equally high, which is comparable to that of the cells frozen by the vitrification method. In the case of vitrification method, it requ

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sprotocols. "A protocol for embryonic stem cell derivation by somatic cell nuclear transfer into human oocytes." December 31, 2014. https://doi.org/10.5281/zenodo.13657.

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Authors: Dieter Egli & Gloryn Chia ### Abstract Here we describe detailed methods that allowed us to derive embryonic stem cell lines by nuclear transfer of fibroblasts from a newborn and from a type 1 diabetic adult. The protocol is based on the insight that 1) agents for cell fusion can act as potent mediators of oocyte activation by compromising maintaining plasma membrane integrity; minimizing the concentration at which they are used, and at least transiently remove calcium from the medium can improve developmental potential. 2) and on the insight that an efficient stimulus for oocyte

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sprotocols. "The use of Optical Magnetic Twisting Cytometry and Flourescence Resonance Energy Transfer to quantify force-induced protein dissociation in the nucleus of a living cell." January 10, 2015. https://doi.org/10.5281/zenodo.13873.

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Authors: Yeh-Chuin Poh & Ning Wang ### Abstract Mechanical forces are known to play a significant role in biological processes. These forces can be transmitted to the cell through the cytoskeletal filament network, inducing different biochemical responses within the cytoplasm. Although there have been ample reports showing that cytoplasmic enzymes can be directly activated by a local stress on the cell surface via integrins, there has been no evidence that mechanical forces can directly alter nuclear functions without intermediate biochemical cascades. Recently, we showed evidence that for

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sprotocols. "High Resolution Melting Analysis for fast and cheap polymorphism screening of marine populations." January 14, 2015. https://doi.org/10.5281/zenodo.13940.

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Authors: Anne-Leila Meistertzheim, Isabelle Calves, Sébastien Artigaud, Carolyn S. Friedman, Christine Paillard, Jean Laroche & Claude Ferec ### Abstract This protocol permits the mutation scanning of PCR products by high-resolution DNA melting analysis requiring the inclusion of a saturating intercalating dye in the PCR mix without labelled probe. During a scanning process, fluorescent melting curves of PCR amplicons are analyzed. Mutations modifying melting curve shapes, are allowed to be further characterized by sequencing because melting is not destructive. The method detects on small

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Dolomatov, S.I., та W. Zukow. "Эпигенетика почек = Kidneys epigenetics". 7 липня 2019. https://doi.org/10.5281/zenodo.3270754.

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<strong>Dolomatov S.I., Zukow W. </strong><strong>Эпигенетика почек</strong><strong> = Kidney</strong><strong>s</strong><strong> epigenetics</strong><strong>. </strong><strong>RSW. Radom,</strong><strong> 144 </strong><strong>p. ISBN </strong><strong>9780359774524</strong><strong>.</strong><strong> DOI </strong><strong>http://dx.doi.org/10.5281/zenodo.3270699</strong><strong> PBN Poland </strong><strong>https://pbn.nauka.gov.pl/sedno-webapp/works/917606</strong>           <strong>Radomska Szkoła Wyższa w Radomiu, Radom, Poland</strong>         &nbsp

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Ameku, W.A., V.N. Ataide, E.T. Costab, et al. "A pencil-lead immunosensor for the rapid electrochemical measurement of anti-Diphtheria Toxin antibodies." October 30, 2021. https://doi.org/10.5281/zenodo.5628477.

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<strong>Abstract: </strong>Diphtheria is a vaccine-preventable disease, yet immunization can wane over time to non-protective levels. We have developed a low-cost, miniaturized electroanalytical biosensor to quantify anti-diphtheria toxin (DTx) immunoglobulin G (IgG) antibody to minimize the risk for localized outbreaks. Two epitopes specific to DTx and recognized by antibodies generated post-vaccination were selected to create a bi-epitope peptide, biEP, by synthesizing the epitopes in tandem. The biEP peptide was conjugated to the surface of a pencil-lead electrode (PLE) integrated into a po

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sprotocols. "Reprogramming human amniotic fluid stem cells to functional pluripotency by manipulation of culture conditions." January 9, 2015. https://doi.org/10.5281/zenodo.13860.

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Authors: Dafni Moschidou & Pascale V Guillot ### Abstract Pluripotent stem cells have potential applications in regenerative medicine, disease modelling and drug screening. Induced pluripotent stem (iPS) cells have first been generated from fibroblasts using retroviral insertion of OCT4A, SOX2, c-MYC and KLF4. Since then, a number of methods have been developed to avoid the random integration of ectopic factors in the genome and the low efficiency of the process. Those include alternative integrating, non-integrating, excisable and DNA-free systems, but they all present challenges that pre

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