Relevant bibliographies by topics / 378 bp DNA fragment

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Academic literature on the topic '378 bp DNA fragment'

Author: Grafiati
Published: 3 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "378 bp DNA fragment"

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WU, Z., I. NAGANO, E. POZIO, and Y. TAKAHASHI. "Polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) for the identification of Trichinella isolates." Parasitology 118, no. 2 (1999): 211–18. http://dx.doi.org/10.1017/s0031182098003679.

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In the present study, polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis was developed to identify 5 species (Trichinella spiralis, Trichinella britovi, Trichinella nativa, Trichinella nelsoni and Trichinella pseudospiralis) and 3 phenotypes of uncertain taxonomic status (Trichinella T5, T6, and T8). Eleven restriction endonucleases were used to restrict 3 DNA fragments (1) a 2800 bp fragment of the 43 kDa excretory–secretory (E–S) protein gene, (2) a 1250 bp fragment amplified with the primer pair SB147A and (3) a 372 bp fragment amplified with the primer p
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Abdel-Rahman, S. M., A. M. Elmaghraby, and A. S. Haggag. "Identification and differentiation among chicken’s, duck’s, quail’s, rabbit’s and turkey's meat using PCR-RFLP technique." Biotehnologija u stocarstvu 31, no. 1 (2015): 101–8. http://dx.doi.org/10.2298/bah1501101a.

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PCR-RFLP technique was developed for identification and differentiation among chicken?s, duck?s, quail?s, rabbit?s and turkey's meat. DNA from small amount of muscles (0.05 g) was extracted and a region of mitochondrial DNA (cytochrome-b gene) in chicken, duck, quail, rabbit and turkey was amplified by PCR. Fragment length of the PCR product was 371 bp in chicken, 374 bp in duck and rabbit and 377 bp in both quail and turkey. Six nucleotides different makes it difficult to differentiate among these five species-specific meat. For differentiation, three different restriction enzymes (DdeI, MspI
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Hawa, N. S., J. L. H. O'Riordan, and S. M. Farrow. "Binding of 1,25-dihydroxyvitamin D3 receptors to the 5′-flanking region of the bovine parathyroid hormone gene." Journal of Endocrinology 142, no. 1 (1994): 53–60. http://dx.doi.org/10.1677/joe.0.1420053.

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Abstract To further define the binding site for receptors for 1,25(OH)2D3 (VDR) in the bovine PTH gene and to study the interactions of transcription factors with VDR, Southwestern and gel shift assays were used. Data from the former indicated binding of VDR to DNA fragments spanning the regions −451 to −348 bp and −668 to −452 bp. Studies using gel shift assays confirmed binding to the −451 to −348 bp fragment and specificity was shown by using excess concentrations of unlabelled −451 to −348 bp fragment to compete for binding, whereas excess unlabelled −347 to +50 bp did not compete. Binding
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Chang, S., ME Reid, J. Conboy, YW Kan, and N. Mohandas. "Molecular characterization of erythrocyte glycophorin C variants." Blood 77, no. 3 (1991): 644–48. http://dx.doi.org/10.1182/blood.v77.3.644.644.

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Abstract Human erythrocyte glycophorin C plays a functionally important role in maintaining erythrocyte shape and regulating membrane mechanical stability. Immunochemical and serologic studies have identified a number of glycophorin C variants that include the Yus, Gerbich, and Webb phenotypes. We report here the molecular characterization of these variants. Amplification of glycophorin C mRNA from the Yus phenotype, using two oligonucleotide primers that span the coding domain, generated a 338-bp fragment compared with a 395-bp fragment generated by amplification of normal glycophorin C mRNA.
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Chang, S., ME Reid, J. Conboy, YW Kan, and N. Mohandas. "Molecular characterization of erythrocyte glycophorin C variants." Blood 77, no. 3 (1991): 644–48. http://dx.doi.org/10.1182/blood.v77.3.644.bloodjournal773644.

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Human erythrocyte glycophorin C plays a functionally important role in maintaining erythrocyte shape and regulating membrane mechanical stability. Immunochemical and serologic studies have identified a number of glycophorin C variants that include the Yus, Gerbich, and Webb phenotypes. We report here the molecular characterization of these variants. Amplification of glycophorin C mRNA from the Yus phenotype, using two oligonucleotide primers that span the coding domain, generated a 338-bp fragment compared with a 395-bp fragment generated by amplification of normal glycophorin C mRNA. Sequenci
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Demeke, T., A. Laroche, and D. A. Gaudet. "A DNA marker for the Bt-10 common bunt resistance gene in wheat." Genome 39, no. 1 (1996): 51–55. http://dx.doi.org/10.1139/g96-007.

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The Bt-10 bunt gene confers resistance to most races of the common bunt fungi, Tilletia tritici and T. laevis. The RAPD technique, employing a total of 965 decamer primers, was used to identify polymorphic markers between resistant (BW553) and susceptible ('Neepawa') near-isogenic lines. Primer 196 (5′ CTC CTC CCC C 3′) produced a 590 base pair (bp) reproducible fragment only in the resistant near-isogenic line. The 590-bp DNA fragment was present in all the 22 wheat cultivars known to carry the Bt-10 resistance gene and also in 15 resistant F2 lines obtained from a cross between the resistant
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Luo, Chao-Xi, Kerik D. Cox, Achour Amiri, and Guido Schnabel. "Occurrence and Detection of the DMI Resistance-Associated Genetic Element ‘Mona’ in Monilinia fructicola." Plant Disease 92, no. 7 (2008): 1099–103. http://dx.doi.org/10.1094/pdis-92-7-1099.

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Sterol demethylation inhibitor (DMI) fungicide resistance in isolates of Monilinia fructicola from Georgia has been linked to overexpression of the MfCYP51 gene and a corresponding 65-bp genetic element ‘Mona’ inserted in the upstream region of MfCYP51. In this study, a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to detect the Mona element. Fourteen DMI fungicide-resistant (DMI-R) and six DMI fungicide-sensitive (DMI-S) isolates from Georgia, six DMI-R and 11 DMI-S isolates from South Carolina, seven DMI-R and nine DMI-S isolates from New
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8

Voevodin, A., E. Samilchuk, J. Allan, J. Rogers, and S. Broussard. "Simian T-lymphotropic virus type 1 (STLV-1) infection in wild yellow baboons (Papio hamadryas cynocephalus) from Mikumi National Park, Tanzania." Virology 228, no. 2 (1997): 350–59. https://doi.org/10.5281/zenodo.13533307.

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(Uploaded by Plazi for the Bat Literature Project) Serum and peripheral blood leukocytes from wild yellow baboons (Papio hamadryas cynocephalus) were tested for the presence of STLV-1-specific antibodies and proviral DNA. Fourteen of 30 sera tested positive by radioimmunoprecipitation assay (RIPA) with HTLV-1. Among 36 DNA samples tested by PCR 15 were positive by double nested PCR for a fragment of the STLV-1 env gene, the most sensitive assay among PCR tests employed. Of 30 animals that were tested both serologically and by PCR in only 1 case were the results discordant (PCR-positive, antibo
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Voevodin, A., E. Samilchuk, J. Allan, J. Rogers, and S. Broussard. "Simian T-lymphotropic virus type 1 (STLV-1) infection in wild yellow baboons (Papio hamadryas cynocephalus) from Mikumi National Park, Tanzania." Virology 228, no. 2 (1997): 350–59. https://doi.org/10.5281/zenodo.13533307.

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(Uploaded by Plazi for the Bat Literature Project) Serum and peripheral blood leukocytes from wild yellow baboons (Papio hamadryas cynocephalus) were tested for the presence of STLV-1-specific antibodies and proviral DNA. Fourteen of 30 sera tested positive by radioimmunoprecipitation assay (RIPA) with HTLV-1. Among 36 DNA samples tested by PCR 15 were positive by double nested PCR for a fragment of the STLV-1 env gene, the most sensitive assay among PCR tests employed. Of 30 animals that were tested both serologically and by PCR in only 1 case were the results discordant (PCR-positive, antibo
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Harju, Susanna, та Kenneth R. Peterson. "In Vivo and In Vitro Analysis of an Aγ-Globin Gene Silencer in Human β-Yac Transgenic Mice." Blood 104, № 11 (2004): 1220. http://dx.doi.org/10.1182/blood.v104.11.1220.1220.

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Abstract Autonomous silencing of gene expression is one mechanism operative in the control of human β-like globin gene switching and is best exemplified by the ε-globin gene. Experiments using variously truncated Aγ-globin genes linked to LCR sequences suggested that a region of the Aγ-globin gene between −730 to −378 relative to the mRNA CAP site may function as an adult stage-specific silencer element. A 5.4 Kb marked Aγ-globin gene (Aγm) inserted between LCR 5′HS1 and the ε-globin gene in a β-YAC (Aγm 5′ ε β-YAC) was silenced in transgenic mice during adult definitive erythropoiesis, even i
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Book chapters on the topic "378 bp DNA fragment"

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"Introduction." In DNA Fingerprinting, edited by Lorne t. Kirby. Oxford University Press, 1993. http://dx.doi.org/10.1093/oso/9780716770015.003.0004.

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DNA identification analysis, identity testing, profiling, fingerprinting, typing, or genotyping refers to the characterization of one or more relatively rare features of an individuals’s genome or hereditary makeup. Every human, lower animal, and sexually reproduced plant has a characteristic phenotype or physical appearance because each possesses a unique hereditary composition. The exception to this rule is identical twins, who possess the same unique genotype but, owing to the consequences of complex developmental events, have subtly different phenotypes. The DNA of any individual is identi
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Niessen, M. L., and R. F. Vogel. "Monitoring of trichothecene producing Fusarium species in brewing cereals using a group specific Polymerase Chain Reaction." In European Brewery Convention. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780199636907.003.0007.

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Abstract A pair of PCR primers (Tox5-1 and Tox5-2) was designed and a PCR set up and optimized. With the primer pair used a 658 bp fragment was amplified from DNA isolated from Fusarium species described as producers of trichothecene mycotoxins. Non-trichothecene producers, cereals, and bacteria gave no signal in the assay. Trichothecene producers were detected in samples of contaminated malts. Results showed that the method developed is group specific for the detection of all Fusarium species capable of producing trichothecenes. The method may be used for quality monitoring in the brewing and
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Leiβner, Christian E. W., Martin L. Niessen, and Rudi F. Vogel. "Nachweis von Fusarium graminearum und Fusarium culmorum unter Verwendung einer sektionsspezifischen Polymerase-kettenreaktion." In European Brewery Convention. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780199636907.003.0008.

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Abstract Fusarium graminearum, F. culmorum, F. avenaceum, and Microdochium nivale are the most serious funga.l contaminants found in cereal grain. F. graminearum and F. culmorum may produce mycotoxins and have been found to be closely linked to the induction of gushing in beer. A pair of PCR primers was designed to amplify a 288 bp fragment from the DNA isolated from fusaria of the Discolor section. This PCR selectively detects F. graminearum and F. culmorum in sample material without being sensitive to other organisms present. The method described can be used in quality control in breweries a
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Guichard, Annabel, Evelyne Bergeret, and Ruth Griffin-Shea. "Rotund Rac-GAP." In Guidebook to the Sinall GTPases. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780198599456.003.0077.

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Abstract The RnRac-GAP coding sequence, originally named pc1.7 and now renamed rnRac-GAP(l), was isolated (Agnel et al. 1989) by screening a cDNA library in Xgt10 prepared from pupae 5-7.5 days after egg laying (Poole etal. 1985) with a genomic fragment from the cloned rn region. Comparison of rnRac-GAP(l) with the corresponding genomic DNA showed the presence of a 482-bp intron at the 5’-end. Sequence and restriction map analysis of the insert of a second cDNA clone, originally named pc1.7d and now renamed rnRac-GAP(2), showed that the two inserts differed only at their 3’-ends.
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Wuster, W., G. M. Salomao, R. S. Thorpe, et al. "Systematics of the Bothrops atrox complex: new insights from multivariate analysis and mitochondrial DNA sequence information." In Venomous Snakes. Oxford University PressOxford, 1996. http://dx.doi.org/10.1093/oso/9780198549864.003.0008.

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Abstract We use multivariate analysis of morphological characters and comparative sequencing of a 520 bp fragment of the cytochrome b gene (mitochondrial DNA) to investigate patterns of geographic variation in the Bothrops atrox species complex, and the population phylogeny of the group in parts of South America. Populations conventionally assigned to B. atrox and B. moojeni constitute morphologically distinct groupings, with a zone of phenetically intermediate specimens where their ranges meet. Bothrops marajoensis, B. isabelae, B. leucurus and B. pradoi are poorly differentiated or undiffere
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"Red Snapper: Ecology and Fisheries in the U.S. Gulf of Mexico." In Red Snapper: Ecology and Fisheries in the U.S. Gulf of Mexico, edited by JOHN R. GOLD and ERIC SAILLANT. American Fisheries Society, 2007. http://dx.doi.org/10.47886/9781888569971.ch13.

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<em>Abstract</em>.—Allelic variation at 19 nuclear-encoded microsatellite loci and haplotype variation in a 590 bp protein-coding fragment of mitochondrial (mt)DNA were assayed among Gulf red snapper sampled from four cohorts at each of three offshore localities (12 samples total) in the northern Gulf of Mexico. Significant heterogeneity in allele and genotype distributions among samples was detected at four microsatellites; six of seven ‘significant’ pairwise comparisons between samples revealed the heterogeneity to be temporal rather than spatial. Nested-clade analysis of mtDNA v
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Conference papers on the topic "378 bp DNA fragment"

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Chen, P. C., D. S. Park, B. H. You, et al. "A High Throughput Microfluidic Thermal Reactor." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-13130.

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A high throughput microfluidic system including a 96 continuous flow (CF) thermal reactors and a multi-zone thermal control system was designed and fabricated. An infrared camera (IR) was used to analyze and verify the uniformity of the temperature distribution. Temperature variations from the nominal values were ±2°C in the denaturation zone and ±1°C in the renaturation and extension zones. Six different DNA fragments, with lengths ranging from 99 bp to 997 bp, were obtained from a λ-DNA template, each with a distinct renaturation temperature. As an initial demonstration of the biochemical pe
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Daly, John, and Mark Davies. "A Quantitative Free Convection DNA Amplifier." In ASME/JSME 2007 Thermal Engineering Heat Transfer Summer Conference collocated with the ASME 2007 InterPACK Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/ht2007-32381.

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The Polymerase Chain Reaction (PCR) has been used extensively to amplify targeted nucleic acids for many applications in molecular biology and, increasingly, in medical diagnostics. Outlined in this paper is a PCR device which takes account of the advantages offered by free convection. The design is, in it fundamental format a time-wise isothermal well-based thermocycler. A temperature gradient induced across the well causes convection forces to circulate the sample through the required temperatures necessary for amplification. Quantitative amplification is demonstrated with real time measurem
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Daly, John, and Mark Davies. "A Natural Convection DNA Amplifier." In ASME 4th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2006. http://dx.doi.org/10.1115/icnmm2006-96244.

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Natural convection is the driver of innumerable natural world phenomena. Within the laboratory, it offers simplified geometries and flow structures without the need for auxiliary flow inducement, thereby greatly reducing the risk of external contamination within biomedical applications. Outlined in this paper is a polymerase chain reaction (PCR) device which takes advantage of these distinct qualities. PCR has become synonymous with DNA amplification in molecular biology laboratories throughout the world, and at the heart of PCR is thermal cycling. Commonly PCR is accomplished utilising a thre
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Nishino, M., T. Nishimura, H. Naka, S. Mikami, A. Yoshioka, and H. Fukui. "CARRIER DETECTION IN JAPANESE FAMILIES WITH HAEMOPHILIA A USING FACTOR VIII GENE PROBE(F8A) AND ST 14-1 PROBE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644009.

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Recently, the gene structure for human F.VIII protein was clarified, and F.VIII DNA probes have been used for carrier detection and prenatal diagnosis ofhaemophilia A. In order to make sure that the phenomena are universal, we have analysed the RFLPs of F.VIII gene in 16 Japanese families with haemophilia A, including a female haemophiliac case, using an intragenic F.VIII DNA probe(F8A) and an extragenic(linked) DNA probe(Stl4-1).The probe F8A revealed two variant bands after digestion by Bel I. Of normal 60 X chromosomes (females) examined, about 85% bore the 879-bp fragment and 15%the 1165-b
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G. V., Izotova, Vlasenko P. G., Kashinskaya E. N., and Solovyev M. M. "IDENTIFICATION OF DIPLOSTOMOSIS PATHOGENS OF PREDOMINATING FISH SPECIES OF SIBERIA USING DNA-BARCODING APPROACH." In II INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE "DEVELOPMENT AND MODERN PROBLEMS OF AQUACULTURE" ("AQUACULTURE 2022" CONFERENCE). DSTU-Print, 2022. http://dx.doi.org/10.23947/aquaculture.2022.53-55.

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Metacercariae of the genus Diplostomum are localized in the eyes and the brain of freshwater fish and cyclostomata species causing different types of diplostomosis. Hyperinvasion by these parasites lead to spatial disorientation, changing in feeding activity, lowering the growth rate, etc. Species identification of Diplostomum spp. based on the morphological features is difficult due to the lack and severity of them. In present study, species diversity of the genus Diplostomum metacercariae parasitizing in fishes of Chany, Baunt and Teletskoye lakes is defined according to the DNA-barcoding ap
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Chelucci, C., H. J. Hassan, R. Guerriero, A. Leonardi, G. Mattia, and C. Peschle. "POLYMORPHIC SITES IN FACTOR X GENE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643836.

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The structure of factor X gene has been analyzed by Southern blot in 5 subjects with factor X deficiency.Genomic DNA was digested with 8 different endonucleases and hybridized with a cDNA probe. The congenital deficiency observed in these patients is not apparently due to a major deletion or rearrangement. Since the gene locus is grossly intact, the disease presumably results from point mutation(s) not identified by the utilized endonucleases.Our study was also focused on the presence of polymorphic site(s) in the factor X gene locus. Analysis of 50 normal subjects allowed to identify several
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R. Abduwahhab ALHEANY, Aya. "GENOTYPING AND SEROPREVALENCE OF TOXOPLASMA GONDII IN ABORTION WITH ELEVATION OF INTERLEUKIN-6 LEVEL." In IV.International Scientific Congress of Pure,Appliedand Technological Sciences. Rimar Academy, 2022. http://dx.doi.org/10.47832/minarcongress4-12.

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Toxoplasmosis is a harmful microorganism that resides inside the cells of the host and produces serious symptoms such as abortion. A total of (48) blood samples were taken from aborted women in this study, with (40) healthy persons serving as a control group. Between the 15th of January and the 30th of September 2021, the patients were treated at Al-Karkh Maternity Hospital. The infection rate was highest in the age range (18-25) years for both the infected women and the control group, according to the findings. Among aborted mothers with Toxoplasmosis, the IgG antibody positivity rate was 40
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Huber, P., J. Dalmon, M. Laurent, G. Courtois, D. Thevenon та G. Marguerie. "CHARACTERIZATION OFTHE 5’FLANKING REGION FOR THE HUMAN FIBRINOGEN β GENE". У XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642889.

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Fibrinogen is coded by three separate genes located in a 50kb region of chromosome 4 and organized in a α - β - γ orientation with an inversion of the gene 3- A human genomic library was constructed using the EMBL4 phage and screened with cDNA probes coding for human fibrinogen Aα, Bβ and γ chains. Clones, covering the fibrinogen locus,were identified, and their organization was analyzed by means of hybridization and restriction mapping. Among these clones one recombinant phage containing the β gene and large 5’ and 3’ -flanking sequences was isolated.To identify the regulatory sequences Dpstr
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