Relevant bibliographies by topics / 3xFLAG-tag

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  1. Journal articles
  2. Dissertations / Theses

Academic literature on the topic '3xFLAG-tag'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "3xFLAG-tag"

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Minh Thu, Doan, Nguyen Thi Minh Viet, and Pham Thi Kim Lien. "Detection of protein stoichiometric phosphorylation using Phos-tag SDS-PAGE." Vietnam Journal of Biotechnology 17, no. 4 (2020): 645–49. http://dx.doi.org/10.15625/1811-4989/17/4/13785.

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Protein phosphorylation plays an important role in many cellular signalings which are relating to many diseases. Therefore, a variety of biochemical techniques has been developed to study protein phosphorylation in cells. Protein phosphorylation has traditionally been detected by radioisotope phosphate labeling of proteins with radioactive ATP. Phosphorylation site-specific antibodies are now available for the analysis of phosphorylation status at target sites. However, these antibodies cannot be used to detect unidentified phosphorylation sites. Recently, the Phos-tag technology has been deve
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Ueda, Minoru, Yoshiyuki Manabe, and Makoto Mukai. "The high performance of 3XFLAG for target purification of a bioactive metabolite: A tag combined with a highly effective linker structure." Bioorganic & Medicinal Chemistry Letters 21, no. 5 (2011): 1359–62. http://dx.doi.org/10.1016/j.bmcl.2011.01.038.

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Klein, Gracjana, Pawel Wojtkiewicz, Daria Biernacka, Anna Stupak, Patrycja Gorzelak, and Satish Raina. "Identification of Substrates of Cytoplasmic Peptidyl-Prolyl Cis/Trans Isomerases and Their Collective Essentiality in Escherichia Coli." International Journal of Molecular Sciences 21, no. 12 (2020): 4212. http://dx.doi.org/10.3390/ijms21124212.

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Protein folding often requires molecular chaperones and folding catalysts, such as peptidyl-prolyl cis/trans isomerases (PPIs). The Escherichia coli cytoplasm contains six well-known PPIs, although a requirement of their PPIase activity, the identity of their substrates and relative enzymatic contribution is unknown. Thus, strains lacking all periplasmic and one of the cytoplasmic PPIs were constructed. Measurement of their PPIase activity revealed that PpiB is the major source of PPIase activity in the cytoplasm. Furthermore, viable Δ6ppi strains could be constructed only on minimal medium in
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4

Okamoto, Yusuke, Masako Abe, Akiko Itaya, et al. "hnRNP U and DDX47 Are Novel FANCD2 Interactors That May Help to Resolve R-Loops during Mild Replication Stress." Blood 132, Supplement 1 (2018): 1301. http://dx.doi.org/10.1182/blood-2018-99-112364.

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Abstract Background: Fanconi anemia proteins, encoded by at least 22genes (FANCA-W), constitute the Interstrand Cross Link (ICL) repair pathway. While FANCD2 is a master regulator of ICL repair, it accumulates at common fragile sites (CFS) during mild replication stress stimulated by low-dose Aphidicolin (APH) treatment. A recent study indicated that FANCD2 is required for efficient genome replication across the CFS regions. FANCD2 is also implicated in the regulation of R-loops levels. R-loops, which consist of DNA: RNA hybrids and displaced single-stranded DNA, are physiologically relevant i
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5

Chang, Chan-Jung, Andriana Kotini, Julie Teruya-Feldstein, Omar Abdel-Wahab, Robert Bradley, and Eirini P. Papapetrou. "Isogenic iPSC Models of SRSF2-Mutant Myelodysplastic Syndrome Capture Disease Phenotypes, Splicing Defects and Drug Responses." Blood 128, no. 22 (2016): 962. http://dx.doi.org/10.1182/blood.v128.22.962.962.

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Abstract A major recent advance for the biology of Myelodysplastic Syndrome (MDS) was the discovery of recurrent mutations in genes encoding splicing factors (SFs). SRSF2 is a SF that binds to RNA sequences called exonic splicing enhancers (ESEs) to promote inclusion of exons containing these motifs. Mutations of SRSF2 are found in 20-30% of MDS patients, are associated with adverse prognosis and are almost always heterozygous missense mutations (P95 L/R/H). This strongly suggests a gain or alteration of function mechanism, corroborated by recent findings by us and others in a knockin mouse mo
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Yuno, Miyuki, Shoko Nagata, Toshitsugu Fujita, and Hodaka Fujii. "MSCV-based retroviral plasmids expressing 3xFLAG-Sp-dCas9 for enChIP analysis." Biology Methods and Protocols 6, no. 1 (2021). http://dx.doi.org/10.1093/biomethods/bpab013.

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Abstract Engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) is a technology for purifying specific genomic regions to facilitate identification of their associated molecules, including proteins, RNAs, and other genomic regions. In enChIP, the target genomic region is tagged with engineered DNA-binding molecules, for example, a variant of the clustered regularly interspaced short palindromic repeats (CRISPR) system consisting of a guide RNA (gRNA) and a catalytically inactive form of Cas9 (dCas9). In this study, to increase the flexibility of enChIP and expand the r
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7

Rozen-Gagnon, Kathryn, Soon Yi, Eliana Jacobson, Sasha Novack, and Charles M. Rice. "A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines." Scientific Reports 11, no. 1 (2021). http://dx.doi.org/10.1038/s41598-020-80436-5.

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AbstractAedes (Ae.) aegypti and Ae. albopictus mosquitoes transmit arthropod-borne diseases around the globe, causing ~ 700,000 deaths each year. Genetic mutants are valuable tools to interrogate both fundamental vector biology and mosquito host factors important for viral infection. However, very few genetic mutants have been described in mosquitoes in comparison to model organisms. The relative ease of applying CRISPR/Cas9-based gene editing has transformed genome engineering and has rapidly increased the number of available gene mutants in mosquitoes. Yet, in vivo studies may not be practic
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Dissertations / Theses on the topic "3xFLAG-tag"

1

Åström, Miranda. "Search for the Argonaute protein that governs miRNA regulation in Dictyostelium discoideum." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-432967.

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MicroRNAs are small non-coding RNAs that regulate gene expression through RNA interference. These small RNAs enact gene silencing by forming a RNA-inducing silencing complex together with the effector protein Argonaute. The function of the Argonautes in the social amoeba Dictyostelium discoideum is not yet fully understood. In this study, we look closer at Argonaute B by investigating if it is possible to extract the protein from the cells by the addition of a polypeptide protein tag called 3xFlag. At the same time, we also look into if Argonaute B is important for cell growth. Sequences of th
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