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Journal articles on the topic '4-methylumbelliferyl'

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Published: 3 June 2025

Last updated: 1 August 2025

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1

Van Roey, P., та J. M. Salerno. "Structure of 4-methylumbelliferyl-β-D-glucopyranoside". Acta Crystallographica Section C Crystal Structure Communications 44, № 5 (1988): 865–67. http://dx.doi.org/10.1107/s0108270188000472.

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2

Bhat, K. M., A. J. Hay, M. Claeyssens та T. M. Wood. "Study of the mode of action and site-specificity of the endo-(1→4)-β-d-glucanases of the fungus Penicillium pinophilum with normal, 1-3H-labelled, reduced and chromogenic cello-oligosaccharides". Biochemical Journal 266, № 2 (1990): 371–78. http://dx.doi.org/10.1042/bj2660371.

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The modes of action of the five major endo-(1→4)-beta-D-glucanases (I, II, III, IV and V) purified from Penicillium pinophilum cellulase were compared by h.p.l.c. analysis, with normal, 1-3H-labelled and reduced cello-oligosaccharides and 4-methylumbelliferyl glycosides as substrates. Significant differences were observed in the preferred site of cleavage even when substrates with the same number of glycosidic bonds were compared. Thus, although endoglucanase I was unable to attack normal cello-oligosaccharides shorter than degree of polymerization 6, it hydrolysed reduced cellopentaose to yie

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3

Szweda, R., U. Spohr, R. U. Lemieux, D. Schindler, D. F. Bishop та R. J. Desnick. "Synthesis of 4-methylumbelliferyl glycosides for the detection of α- and β-D-galactopyranosaminidases". Canadian Journal of Chemistry 67, № 9 (1989): 1388–91. http://dx.doi.org/10.1139/v89-213.

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Reaction at room temperature of either 3,4,6-tri-O-acetyl-2-azido-2-deoxy-α- or -β-D-galactopyranosyl chloride with a twofold excess of 4-methylumbelliferone and silver trifluoromethanesulfonate in dichloromethane containing an equimolar amount of.sym-collidine yielded 4-methylumbelliferyl tri-O-acetyl-2-azido-2-deoxy-α-D-galactopyranoside in 33% yield. The β anomer was formed in 20 and 10% yields, respectively. Reduction of the azido group, acetylation followed by de-O-acetylation, provided the desired 4-methylumbelliferyl 2-acetamido-2-deoxy-α- and -β-D-galactopyranosides (N-acetyl-α- and -β

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4

Beccari, T., C. Emiliani, R. Hosseini, A. Orlacchio та J. L. Stirling. "Intermediate forms of human β-N-acetylhexosaminidase lack activity towards 4-methylumbelliferyl β-N-acetylglucosaminide 6-sulphate". Biochemical Journal 244, № 3 (1987): 801–4. http://dx.doi.org/10.1042/bj2440801.

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4-Methylumbelliferyl beta-N-acetylglucosaminide 6-sulphate was purified from a mixture containing its unsulphated precursor. The substrate was used to test for the presence of functional alpha-subunits in ‘intermediate’ forms of human beta-N-acetylhexosaminidase in samples of normal and pregnancy serum and in extracts of placenta and lymphocytes from a patient with common acute lymphoblastic leukaemia. Intermediate forms in these samples had no activity towards 4-methylumbelliferyl beta-N-acetylglucosaminide 6-sulphate, indicating that they lack alpha-subunits.

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5

Nagy, Nadine, Irina Gurevich, Hedwich F. Kuipers, et al. "4-Methylumbelliferyl glucuronide contributes to hyaluronan synthesis inhibition." Journal of Biological Chemistry 294, no. 19 (2019): 7864–77. http://dx.doi.org/10.1074/jbc.ra118.006166.

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6

Honda, Y., M. Kirihata, T. Fukamizo, S. Kaneko, K. Tokuyasu, and R. Brzezinski. "Chitosanase-Catalyzed Hydrolysis of 4-Methylumbelliferyl -Chitotr ioside." Journal of Biochemistry 126, no. 3 (1999): 470–74. http://dx.doi.org/10.1093/oxfordjournals.jbchem.a022475.

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7

Zeng, Xiaoxiong, Yi Sun та Hirotaka Uzawa. "Efficient Enzymatic Synthesis of 4-methylumbelliferyl N-acetyllactosaminide and 4-methylumbelliferyl sialyl N -acetyllactosaminides Employing β-D-galactosidase and Sialyltransferases". Biotechnology Letters 27, № 19 (2005): 1461–65. http://dx.doi.org/10.1007/s10529-005-1310-3.

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8

Hassan, Abdullah A., and Stefan Oscarson. "Facile anomer-oriented syntheses of 4-methylumbelliferyl sialic acid glycosides." Organic & Biomolecular Chemistry 19, no. 30 (2021): 6644–49. http://dx.doi.org/10.1039/d1ob00877c.

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9

OHTA, NAOKO, TOSHIHISA YOTSUYANAGI, and KEN IKEDA. "Degradation of 4'-methylumbelliferyl 4-guanidinobenzoate catalyzed by human serum albumin." CHEMICAL & PHARMACEUTICAL BULLETIN 36, no. 6 (1988): 2152–57. http://dx.doi.org/10.1248/cpb.36.2152.

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10

Vaneechoutte, M., G. Verschraegen, G. Claeys, and P. Flamen. "Rapid identification of Branhamella catarrhalis with 4-methylumbelliferyl butyrate." Journal of Clinical Microbiology 26, no. 6 (1988): 1227–28. http://dx.doi.org/10.1128/jcm.26.6.1227-1228.1988.

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11

Fiksdal, L., I. Tryland, and H. Nelis. "Rapid detection of coliform bacteria and influence of non-target bacteria." Water Science and Technology 35, no. 11-12 (1997): 415–18. http://dx.doi.org/10.2166/wst.1997.0769.

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Enzymatic hydrolysis of fluorogenic substrates (4-methylumbelliferyl-β-D-galactoside, 4-methylumbelliferyl-β-D-glucuronide) has been used for rapid (25min) detection of indicators of faecal water pollution, i.e. coliform and thermotolerant coliform bacteria. In the present work, enzymatic activities and different groups of bacteria (i.e. thermotolerant coliforms, coliforms, β-galactosidase positive and β-glucuronidase positive bacteria) from sewage effluent and polluted river water have been investigated. Ratios of the order of 1:10 between coliforms and β-galactosidase positive bacteria were

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12

Beccari, T., A. Orlacchio та J. L. Stirling. "Identification of β-N-acetylhexosaminidase A in mouse tissues with the fluorigenic substrate 4-methylumbelliferyl-β-N-acetylglucosamine 6-sulphate". Biochemical Journal 252, № 2 (1988): 617–20. http://dx.doi.org/10.1042/bj2520617.

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beta-N-Acetylhexosaminidase from mouse tissue was separated into its constituent isoenzymes on DEAE-cellulose and its activity was monitored with 4-methylumbelliferyl-beta-N-acetylglucosamine and 4-methylumbelliferyl-beta-N-acetylglucosamine 6-sulphate. Forms corresponding to the human isoenzymes A (acidic), B (basic) and an ‘intermediate’ form were present in mouse liver and spleen, whereas in kidney the B and ‘intermediate’ forms predominated, with A present only as a minor component. In brain the ‘intermediate’, A and C activities were detected. Testis had predominantly A activity, whereas

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13

Olsson, M., A. Syk, and R. Wollin. "Identification of Salmonellae with the 4-methylumbelliferyl caprilate fluorescence test." Journal of Clinical Microbiology 29, no. 11 (1991): 2631–32. http://dx.doi.org/10.1128/jcm.29.11.2631-2632.1991.

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14

Freeman, Stuart J., Prema Shankaran, Leonhard S. Wolfe, and John W. Callahan. "Phosphatidylcholine and 4-methylumbelliferyl phosphorylcholine hydrolysis by purified placental sphingomyelinase." Canadian Journal of Biochemistry and Cell Biology 63, no. 4 (1985): 272–77. http://dx.doi.org/10.1139/o85-040.

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We present evidence which indicates that highly purified placental acid sphingomyelinase hydrolyses [14C]phosphatidylcholine ([14C]PC) and the synthetic phosphodiester 4-methylumbelliferyl phosphorylcholine (4-MUPC). Hydrolysis was achieved by phospholipase C phosphodiesterase action. Of the several detergents tested, sodium taurocholate alone was necessary for PC hydrolysis, while 4-MUPC was hydrolysed independent of any detergent requirement. The pH optima for the reactions were 4.6–4.8 for PC hydrolysis and 4.8–5.0 for 4-MUPC hydrolysis. As with sphingomyelin hydrolysis, degradation of both

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15

Van Lieshout, J., M. Faijes, J. Nieto, J. Van Der Oost та A. Planas. "Hydrolase and glycosynthase activity of endo-1,3-β-glucanase from the thermophilePyrococcus furiosus". Archaea 1, № 4 (2004): 285–92. http://dx.doi.org/10.1155/2004/731548.

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Pyrococcus furiosuslaminarinase (LamA, PF0076) is an endo-glycosidase that hydrolyzes β-1,3-gluco-oligosaccharides, but not β-1,4-gluco-oligosaccharides. We studied the specificity of LamA towards small saccharides by using 4-methylumbelliferyl β-glucosides with different linkages. Besides endo-activity, wild-type LamA has some exo-activity, and catalyzes the hydrolysis of mixed-linked oligosaccharides (Glcβ4Glcβ3Glcβ-MU (Glc = glucosyl, MU = 4-methylumbelliferyl)) with both β-1,4 and β-1,3 specificities. The LamA mutant E170A had severely reduced hydrolytic activity, which is consistent with

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16

Emiliani, C., F. Falzetti, A. Orlacchio та J. L. Stirling. "Treatment of HL-60 cells with dimethyl sulphoxide inhibits the formation of β-N-acetylhexosaminidase S". Biochemical Journal 272, № 1 (1990): 211–15. http://dx.doi.org/10.1042/bj2720211.

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beta-N-Acetylhexosaminidase of HL-60 cells was separated into two main forms, A and S, by chromatography on DEAE-cellulose. Analysis of developmental changes in the isoenzyme pattern was complicated by the fact that the specific activity of beta-N-acetylhexosaminidase underwent a 6-fold change during the normal growth cycle. Two other lysosomal enzymes, beta-galactosidase and alpha-mannosidase, behaved similarly. Induction of differentiation of HL-60 cells with dimethyl sulphoxide at a low cell density (3 x 10(5) cells/ml) had a greater effect on the abundance of alpha-subunits of beta-N-acety

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17

Shadix, Lois C., Michele E. Dunnigan та Eugene W. Rice. "Detection of Escherichia coli by the nutrient agar plus 4-methylumbelliferyl β-D-glucuronide (MUG) membrane filter method". Canadian Journal of Microbiology 39, № 11 (1993): 1066–70. http://dx.doi.org/10.1139/m93-161.

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A two-step membrane filter procedure was evaluated to determine the ability to differentiate Escherichia coli from other coliform bacteria recovered from water. M-Endo LES agar incubated at 35 °C for 24 ± 2 h was used as the initial isolation medium. Membranes containing coliform colonies were transferred to nutrient agar plus 4-methylumbelliferyl β-D-glucuronide (MUG) and incubated for an additional 4 h at 35 °C. Escherichia coli colonies were distinguished by fluorescence when viewed under a long-wavelength ultraviolet light. A total of 119 MUG-positive colonies were isolated from 15 water s

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18

Ding, Haixin, Qiang Xiao, Jiameng Tian та ін. "Practical Synthesis of the Fluorogenic Enzyme Substrate 4-Methylumbelliferyl α-l-Idopyranosiduronic Acid". Synlett 31, № 11 (2020): 1083–86. http://dx.doi.org/10.1055/s-0040-1708021.

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A practical and concise synthesis of 4-methylumbelliferyl α-l-idopyranosiduronic acid, a fluorogenic enzyme substrate diagnostic for α-l-iduronidase, was accomplished. It features successive radical bromination and radical reduction of easily accessible methyl 4-methylumbelliferyl-2,3,4-tri-O-acetyl-β-d-glucouronate in four steps with 28% overall yield.

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19

Barth, M. G. M., and P. D. Bridge. "4-Methylumbelliferyl substituted compounds as fluorogenic substrates for fungal extracellular enzymes." Letters in Applied Microbiology 9, no. 5 (1989): 177–79. http://dx.doi.org/10.1111/j.1472-765x.1989.tb00318.x.

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20

Shea Miller, S., R. G. Fulcher, and Illimar Altosaar. "Evaluation of 4-methylumbelliferyl heptanoate as a substrate for oat lipase." Journal of Cereal Science 10, no. 1 (1989): 61–68. http://dx.doi.org/10.1016/s0733-5210(89)80035-9.

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21

Al-Kady, Ahmed S., El-Sadat I. Ahmed, M. Gaber, Mohamed M. Hussein, and El-Zeiny M. Ebeid. "Kinetics of catalyzed hydrolysis of 4-methylumbelliferyl caprylate (MUCAP) salmonella reagent." Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 79, no. 5 (2011): 1540–45. http://dx.doi.org/10.1016/j.saa.2011.05.013.

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22

Ramkumar, R., A. Surolia, and S. K. Podder. "Energetics of carbohydrate binding by a 14 kDa S-type mammalian lectin." Biochemical Journal 308, no. 1 (1995): 237–41. http://dx.doi.org/10.1042/bj3080237.

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The thermodynamics of the binding of derivatives of galactose and lactose to a 14 kDa beta-galactoside-binding lectin (L-14) from sheep spleen has been studied in 10 nM phosphate/150 mM NaCl/10 mM beta-mercaptoethanol buffer, pH 7.4, and in the temperature range 285-300 K using titration calorimetry. The single-site binding constants of various sugars for the lectin were in the following order: N-acetyl-lactosamine thiodigalactoside > 4-methylumbelliferyl lactoside > lactose > 4-methylumbelliferyl alpha-D-galactoside > methyl-alpha-galactose > methyl-beta-galactose. Reactions we

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23

Markina, N. A., та Ya V. Voznyi. "A short synthesis of 4-deoxy-4-fluoroglucosaminides: Methylumbelliferyl N-acetyl-4-deoxy-4-fluoro-β-D-glucosaminide". Russian Journal of Bioorganic Chemistry 34, № 4 (2008): 475–79. http://dx.doi.org/10.1134/s1068162008040122.

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24

Adcock, Philip W., and Christopher P. Saint. "Rapid Confirmation of Clostridium perfringens by Using Chromogenic and Fluorogenic Substrates." Applied and Environmental Microbiology 67, no. 9 (2001): 4382–84. http://dx.doi.org/10.1128/aem.67.9.4382-4384.2001.

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ABSTRACT The use of 4-methylumbelliferyl phosphate (MUP) andortho-nitrophenyl-β-d-galactopyranoside (ONPG) for the identification of Clostridium perfringenswas investigated. A liquid assay containing both MUP and ONPG was a highly specific alternative method for C. perfringensconfirmation, reducing incubation time from 48 to only 4 h. The assay solution is easy to prepare, does not require anaerobic conditions for use, and has an extended shelf life.

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25

Luyten, G. P., A. T. Hoogeveen, and H. Galjaard. "A fluorescence staining method for the demonstration and measurement of lysosomal enzyme activities in single cells." Journal of Histochemistry & Cytochemistry 33, no. 9 (1985): 965–68. http://dx.doi.org/10.1177/33.9.3926869.

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A cytochemical fluorescence method is described that makes possible simple, rapid, and specific demonstration and measurement of the activities of a wide variety of lysosomal enzymes in single cells using 4-methylumbelliferyl derivatives as substrates. The validity of the method and a number of applications using normal and mutant human cells are presented.

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26

Shadix, Lois C., та Eugene W. Rice. "Evaluation of β-glucuronidase assay for the detection of Escherichia coli from environmental waters". Canadian Journal of Microbiology 37, № 12 (1991): 908–11. http://dx.doi.org/10.1139/m91-157.

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The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on β-glucuronidase activity, using the fluorogenic substrate 4-methylumbelliferyl β-D-glucuronide (MUG). This study was conducted to determine whether β-glucuronidase negative E. coli were present in significant numbers in environmental waters. Two hundred and forty E. coli cultures were isolated from 12 water samples collected from differen

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27

Thompson, Hayley, Karen A. Homer, Susmitha Rao, Veronica Booth, and Arthur H. F. Hosie. "An Orthologue of Bacteroides fragilis NanH Is the Principal Sialidase in Tannerella forsythia." Journal of Bacteriology 191, no. 11 (2009): 3623–28. http://dx.doi.org/10.1128/jb.01618-08.

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ABSTRACT Sialidase activity is a putative virulence factor of the anaerobic periodontal pathogen Tannerella forsythia, but it is uncertain which genes encode this activity. Characterization of a putative sialidase, SiaHI, by others, indicated that this protein alone may not be responsible for all of the sialidase activity. We describe a second sialidase in T. forsythia (TF0035), an orthologue of Bacteroides fragilis NanH, and its expression in Escherichia coli. Sialidase activity of the expressed NanH was confirmed by using 2′-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid as a substrate.

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28

Tian, Jiameng, Qianqian Ning, Haixin Ding, Jiang Bai та Qiang Xiao. "Synthesis of Enzyme Substrate 6-Chloro-4-methylumbelliferyl-α-L-idopyranosiduronic Acid". Chinese Journal of Organic Chemistry 40, № 1 (2020): 215. http://dx.doi.org/10.6023/cjoc201908008.

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29

Imamura, Akihiro, Hiromune Ando, Hideharu Ishida та Makoto Kiso. "Di-tert-butylsilylene-Directed α-Selective Synthesis of 4-Methylumbelliferyl T-Antigen". Organic Letters 7, № 20 (2005): 4415–18. http://dx.doi.org/10.1021/ol051592z.

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30

Fiksdal, L., M. Pommepuy, A. Derrien, and M. Cormier. "Production of 4-methylumbelliferyl heptanoate hydrolase by Escherichia coli exposed to seawater." Applied and Environmental Microbiology 55, no. 9 (1989): 2424–27. http://dx.doi.org/10.1128/aem.55.9.2424-2427.1989.

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31

Bussink, Anton P., Marri Verhoek, Jocelyne Vreede, et al. "Common G102S polymorphism in chitotriosidase differentially affects activity towards 4-methylumbelliferyl substrates." FEBS Journal 276, no. 19 (2009): 5678–88. http://dx.doi.org/10.1111/j.1742-4658.2009.07259.x.

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32

Huang, G. "Synthesis and stability assay of 4-methylumbelliferyl (1→3)-β-D-pentaglucoside". Journal of Enzyme Inhibition and Medicinal Chemistry 24, № 2 (2008): 453–56. http://dx.doi.org/10.1080/14756360802188545.

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33

Kirsch, Gilbert, Miguel López-López, Alexander Balbuzano-Deus та ін. "A New Synthetic Route to 4-Methylumbelliferyl-β-d-glucopyranosiduronic Acid (MUG)". Synlett 2007, № 04 (2007): 0649–51. http://dx.doi.org/10.1055/s-2007-967972.

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34

Urano, Tetsumei, Shoko Urano, and Francis J. Castellino. "Reaction of tissue-type plasminogen activator with 4-methylumbelliferyl-p-guanidinobenzoate hydrochloride." Biochemical and Biophysical Research Communications 150, no. 1 (1988): 45–51. http://dx.doi.org/10.1016/0006-291x(88)90484-6.

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35

Wang, Lai-Xi, Nadejda V. Pavlova, Su-Chen Li, Yu-Teh Li, and Yuan C. Lee. "A fluorometric assay of ceramide glycanase with 4-methylumbelliferyl ?-d-lactoside derivatives." Glycoconjugate Journal 13, no. 3 (1996): 359–65. http://dx.doi.org/10.1007/bf00731468.

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36

Dalton, Maurice T., David J. M. Haldane та June MacDonald. "Rapid identification of Candida albicans using 4-methylumbelliferyl N-acetyl-β-galactosaminide". Diagnostic Microbiology and Infectious Disease 12, № 6 (1989): 521–23. http://dx.doi.org/10.1016/0732-8893(89)90087-4.

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37

Anthony, Frank A., Dennis L. Merat, and Wai Yiu Cheung. "A spectrofluorimetric assay of calmodulin-dependent protein phosphatase using 4-methylumbelliferyl phosphate." Analytical Biochemistry 155, no. 1 (1986): 103–7. http://dx.doi.org/10.1016/0003-2697(86)90232-0.

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38

Marsh, Jane, та A. H. Fensom. "4-methylumbelliferyl α-N-acetylglucosaminidase activity for diagnosis of Sanfilippo B disease". Clinical Genetics 27, № 3 (2008): 258–62. http://dx.doi.org/10.1111/j.1399-0004.1985.tb00217.x.

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39

Huang, G. "A Facile Synthesis of 4-Methylumbelliferyl (1-->3)-β-D-Pentaglucoside." Letters in Drug Design & Discovery 5, no. 6 (2008): 362–63. http://dx.doi.org/10.2174/157018008785777298.

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40

Oh, Se-Wook, and Dong-Hyun Kang. "Fluorogenic Selective and Differential Medium for Isolation of Enterobacter sakazakii." Applied and Environmental Microbiology 70, no. 9 (2004): 5692–94. http://dx.doi.org/10.1128/aem.70.9.5692-5694.2004.

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ABSTRACT 4-Methylumbelliferyl-α-d-glucoside, the fluorogenic substrate of α-glucosidase, was used as a selective marker to develop a differential medium for Enterobacter sakazakii. This bacterium showed strong fluorogenic characteristics clearly distinguishable from other microorganisms. On the basis of reducing background noise, an optimum basal medium and nitrogen source were selected. Incubation conditions were optimized.

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41

Unc, Adrian, Julie Gardner, and Susan Springthorpe. "Recovery of Escherichia coli from Soil after Addition of Sterile Organic Wastes." Applied and Environmental Microbiology 72, no. 3 (2006): 2287–89. http://dx.doi.org/10.1128/aem.72.3.2287-2289.2006.

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ABSTRACT Laboratory batch tests indicate that addition of sterile municipal sewage biosolids to clay soil from four depths increases the numbers of Escherichia coli isolates recoverable in EC-MUG broth (EC broth with 4-methylumbelliferyl-β-glucuronide). This effect was most marked for the deeper soil layers, with increases of about 2.6 orders of magnitude in E. coli most probable number.

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42

Tada, Y., S. Sekiguchi, F. Ito, and Y. Eto. "4-Methylumbelliferyl Lipase in Human and Mouse Brain: A Possible Localization in Myelin." Journal of Neurochemistry 46, no. 1 (1986): 140–43. http://dx.doi.org/10.1111/j.1471-4159.1986.tb12936.x.

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43

Mitra, D., та M. Sarkar. "Physicochemical studies of binding of 4-methylumbelliferyl β-d-galactopyranoside to cold agglutinin". Biochemical Journal 262, № 1 (1989): 357–60. http://dx.doi.org/10.1042/bj2620357.

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The fluorescence of 4-methylumbelliferyl beta-D-galactopyranoside (MeUmbGalp) was quenched in the presence of cold agglutinin, showing that there was binding between MeUmbGalp and cold agglutinin. That binding was saccharide-specific. By using this quenching phenomenon, the association constants (Ka) of the binding of cold agglutinin at different temperatures (10 degrees C and 15 degrees C) to MeUmbGalp and also the number of binding sites were calculated. The Ka values were found to be 2.63 x 10(3) M-1 at 10 degrees C and 1.58 x 10(3) M-1 at 15 degrees C. Though there is a change in Ka values

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44

Broadhead, D. M., та J. Butterworth. "Pompe's disease: Diagnosis in kidney and leucocytes using 4-methylumbelliferyl-α-D-glucopyranoside". Clinical Genetics 13, № 6 (2008): 504–10. http://dx.doi.org/10.1111/j.1399-0004.1978.tb01206.x.

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45

DURANT, PAMELA J., MARILYN S. BARTLETT, MARGARET M. SHAW, SHERRY F. QUEENER, and JAMES W. SMITH. "Demonstration of Esterase Activity in Pneumocystis carinii by Cleavage of 4-Methylumbelliferyl Substrates." Journal of Eukaryotic Microbiology 43, no. 5 (1996): 45S. http://dx.doi.org/10.1111/j.1550-7408.1996.tb04981.x.

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46

Berthelot, Karine, та Francis M. Delmotte. "Purification and Characterization of an α-Glucosidase from Rhizobium sp. (Robinia pseudoacacia L.) Strain USDA 4280". Applied and Environmental Microbiology 65, № 7 (1999): 2907–11. http://dx.doi.org/10.1128/aem.65.7.2907-2911.1999.

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ABSTRACT A novel α-glucosidase with an apparent subunit mass of 59 ± 0.5 kDa was purified from protein extracts of Rhizobium sp. strain USDA 4280, a nodulating strain of black locust (Robinia pseudoacacia L), and characterized. After purification to homogeneity (475-fold; yield, 18%) by ammonium sulfate precipitation, cation-exchange chromatography, hydrophobic chromatography, dye chromatography, and gel filtration, this enzyme had a pI of 4.75 ± 0.05. The enzyme activity was optimal at pH 6.0 to 6.5 and 35°C. The activity increased in the presence of NH4 +and K+ ions but was inhibited by Cu2+

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Miller, Morten, Ansa Palojärvi, Andrea Rangger, Morten Reeslev, and Annelise Kjøller. "The Use of Fluorogenic Substrates To Measure Fungal Presence and Activity in Soil." Applied and Environmental Microbiology 64, no. 2 (1998): 613–17. http://dx.doi.org/10.1128/aem.64.2.613-617.1998.

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ABSTRACT Our objective was to determine if 4-methylumbelliferyl-labelled enzyme substrates could be used to detect and quantify specific components of chitinase and cellulase activities as specific indicators of the presence and activity of fungal biomass. The fluorogenic substrates 4-methylumbelliferyl (MUF)N-acetyl-β-d-glucosaminide and MUF β-d-lactoside were used for the detection and quantification of β-N-acetylglucosaminidase (EC 3.2.1.30 ) (NAGase) and endo 1,4-β-glucanase (EC 3.2.1.4 )/cellobiohydrolase (EC3.2.1.91 ) (CELase), respectively. Culture screenings on solid media showed a wid

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Wang, L. X., N. O. Keyhani, S. Roseman, and Y. C. Lee. "4-Methylumbelliferyl glycosides of N-acetyl 4-thiochito-oligosaccharides as fluorogenic substrates for chitodextrinase from Vibrio furnissii." Glycobiology 7, no. 6 (1997): 855–60. http://dx.doi.org/10.1093/glycob/7.6.855.

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Yang, Xiao-Feng, Zhuan Su, Chenghai Liu, Haiping Qi, and Minglei Zhao. "A thiol-selective fluorogenic probe based on the cleavage of 4-methylumbelliferyl-2’,4’,6’-trinitropheyl ether." Analytical and Bioanalytical Chemistry 396, no. 7 (2010): 2667–74. http://dx.doi.org/10.1007/s00216-010-3475-4.

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Garcia-Sastre, A., E. Villar, J. C. Manuguerra, C. Hannoun, and J. A. Cabezas. "Activity of influenza C virus O-acetylesterase with O-acetyl-containing compounds." Biochemical Journal 273, no. 2 (1991): 435–41. http://dx.doi.org/10.1042/bj2730435.

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Influenza C virus (strain C/Johannesburg/1/66) was grown, harvested, purified and used as source for the enzyme O-acetylesterase (N-acyl-O-acetylneuraminate O-acetylhydrolase; EC 3.1.1.53). This activity was studied and characterized with regard to some new substrates. The pH optimum of the enzyme is around 7.6, its stability at different pH values shows a result similar to that of the pH optimum, and its activity is well maintained in the pH range from 7.0 to 8.5 (all these tests were performed with 4-nitrophenyl acetate as substrate). Remarkable differences were found in the values of both K

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