Academic literature on the topic '45S ribosomal RNA genes (45S rDNA)'

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Journal articles on the topic "45S ribosomal RNA genes (45S rDNA)"

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Delorme-Hinoux, Valérie, Assane Mbodj, Sophie Brando, et al. "45S rDNA Diversity In Natura as One Step towards Ribosomal Heterogeneity in Arabidopsis thaliana." Plants 12, no. 14 (2023): 2722. http://dx.doi.org/10.3390/plants12142722.

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The keystone of ribosome biogenesis is the transcription of 45S rDNA. The Arabidopsis thaliana genome contains hundreds of 45S rDNA units; however, they are not all transcribed. Notably, 45S rDNA units contain insertions/deletions revealing the existence of heterogeneous rRNA genes and, likely, heterogeneous ribosomes for rRNAs. In order to obtain an overall picture of 45S rDNA diversity sustaining the synthesis of rRNAs and, subsequently, of ribosomes in natura, we took advantage of 320 new occurrences of Arabidopsis thaliana as a metapopulation named At66, sampled from 0 to 1900 m of altitud
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Tran, Trang Thi Quynh, Trang Hien Do, Tung The Pham, et al. "Hypermethylation at 45S rDNA promoter in cancers." PLOS ONE 20, no. 1 (2025): e0311085. https://doi.org/10.1371/journal.pone.0311085.

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The ribosomal genes (rDNA genes) encode 47S rRNA which accounts for up to 80% of all cellular RNA. At any given time, no more than 50% of rDNA genes are actively transcribed, and the other half is silent by forming heterochromatin structures through DNA methylation. In cancer cells, upregulation of ribosome biogenesis has been recognized as a hallmark feature, thus, the reduced methylation of rDNA promoter has been thought to support conformational changes of chromatin accessibility and the subsequent increase in rDNA transcription. However, an increase in the heterochromatin state through rDN
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Gokhman, Vladimir E., and Valentina G. Kuznetsova. "Structure and Evolution of Ribosomal Genes of Insect Chromosomes." Insects 15, no. 8 (2024): 593. http://dx.doi.org/10.3390/insects15080593.

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Currently, clusters of 45S and 5S ribosomal DNA (rDNA) have been studied in about 1000 and 100 species of the class Insecta, respectively. Although the number of insect species with known 45S rDNA clusters (also referred to as nucleolus-organizing regions, or NORs) constitutes less than 0.1 percent of the described members of this enormous group, certain conclusions can already be drawn. Since haploid karyotypes with single 45S and 5S rDNA clusters predominate in both basal and derived insect groups, this character state is apparently ancestral for the class Insecta in general. Nevertheless, t
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Bi, Ke, James P. Bogart, and Jinzhong Fu. "A populational survey of 45S rDNA polymorphism in the Jefferson salamander Ambystoma jeffersonianum revealed by fluorescence in situ hybridization (FISH)." Current Zoology 55, no. 2 (2009): 145–49. http://dx.doi.org/10.1093/czoolo/55.2.145.

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Abstract The chromosomal localization of 45S ribosomal RNA genes in Ambystoma jeffersonianum was determined by fluorescence in situ hybridization with 18S rDNA fragment as a probe (FISH-rDNA). Our results revealed the presence of rDNA polymorphism among A. jeffersonianum populations in terms of number, location and FISH signal intensity on the chromosomes. Nine rDNA cytotypes were found in ten geographically isolated populations and most of them contained derivative rDNA sites. Our preliminary study provides strong indication of karyotypic diversification of A. Jeffersonianum that is demonstra
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Michalak, Katarzyna, Sebastian Maciak, Young Bun Kim, et al. "Nucleolar dominance and maternal control of 45S rDNA expression." Proceedings of the Royal Society B: Biological Sciences 282, no. 1820 (2015): 20152201. http://dx.doi.org/10.1098/rspb.2015.2201.

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Using a system of interspecies hybrids, trihybrids, and recombinants with varying proportions of genomes from three distinct Xenopus species, we provide evidence for de novo epigenetic silencing of paternal 45S ribosomal ribonucleic acid (rRNA) genes and their species-dependent expression dominance that escapes transcriptional inactivation after homologous recombination. The same pattern of imprinting is maintained in the offspring from mothers being genetic males (ZZ) sex-reversed to females, indicating that maternal control of ribosomal deoxyribonucleic acid (rDNA) expression is not sex-chro
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De, Bustos Alfredo. "Comparative FISH mapping of 45S and 5S rDNA in the genus Gambierdiscus advances understanding of the cytogenetic diversity and mitosis of dinoflagellates." European Journal of Phycology 57 (September 28, 2021): 264–76. https://doi.org/10.1080/09670262.2021.1964608.

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Dinoflagellates are protists with exceptionally large genomes and chromosomes that lack nucleosomes. To date, information on their genome organization is scarce. Studies investigating the distribution of ribosomal RNA genes (rDNA) in plants and animals by fluorescence in situ hybridization (FISH) have provided important information to elucidate the genome organization and evolution of many species. In the present study, FISH was used to analyse the chromosomal organization of 45S and 5S rDNA and the extent of rDNA variation among five species of Gambierdiscus (G. australes, G. excentricus
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Abirached-Darmency, Mona, Emilce Prado-Vivant, Liudmila Chelysheva, and Thomas Pouthier. "Variation in rDNA locus number and position among legume species and detection of 2 linked rDNA loci in the model Medicago truncatula by FISH." Genome 48, no. 3 (2005): 556–61. http://dx.doi.org/10.1139/g05-015.

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Within Fabaceae, legume species have a variable genome size, chromosome number, and ploidy level. The genome distribution of ribosomal genes, easily detectable by fluorescent in situ hybridization (FISH), is a good tool for anchoring physical and genetic comparative maps. The organisation of 45S rDNA and 5S loci was analysed by FISH in the 4 closely related species: Pisum sativum, Medicago truncatula, Medicago sativa (2 diploid taxa), and Lathyrus sativus. The 2 types of rDNA arrays displayed interspecific variation in locus number and location, but little intraspecific variation was detected.
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Dyomin, Alexander, Svetlana Galkina, Arina Ilina, and Elena Gaginskaya. "Single Copies of the 5S rRNA Inserted into 45S rDNA Intergenic Spacers in the Genomes of Nototheniidae (Perciformes, Actinopterygii)." International Journal of Molecular Sciences 24, no. 8 (2023): 7376. http://dx.doi.org/10.3390/ijms24087376.

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In the vast majority of Animalia genomes, the 5S rRNA gene repeats are located on chromosomes outside of the 45S rDNA arrays of the nucleolar organiser (NOR). We analysed the genomic databases available and found that a 5S rDNA sequence is inserted into the intergenic spacer (IGS) between the 45S rDNA repeats in ten species of the family Nototheniidae (Perciformes, Actinopterigii). We call this sequence the NOR-5S rRNA gene. Along with Testudines and Crocodilia, this is the second case of a close association between four rRNA genes within one repetitive unit in deuterostomes. In both cases, NO
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Coluccia, Elisabetta, Federica Deidda, Cinzia Lobina, et al. "Chromosome Mapping of 5S Ribosomal Genes in Indo-Pacific and Atlantic Muraenidae: Comparative Analysis by Dual Colour Fluorescence In Situ Hybridisation." Genes 11, no. 11 (2020): 1319. http://dx.doi.org/10.3390/genes11111319.

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The Muraenidae is one of the largest and most complex anguilliform families. Despite their abundance and important ecological roles, morays are little studied, especially cytogenetically, and both their phylogenetic relationships and the taxonomy of their genera are controversial. With the aim of extending the karyology of this fish group, the chromosomal mapping of the 5S ribosomal gene family was performed on seven species belonging to the genera Muraena and Gymnothorax from both the Atlantic and Pacific oceans. Fluorescence in situ hybridisation (FISH) experiments were realized using specie
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Choi, Young A., Ryutaro Tao, Keizo Yonemori, and Akira Sugiura. "Simultaneous Visualization of 5S and 45S rDNAs in Persimmon (Diospyros kaki) and Several Wild Relatives (Diospyros spp.) by Fluorescent in situ Hybridization (FISH) and MultiColor FISH (MCFISH)." Journal of the American Society for Horticultural Science 128, no. 5 (2003): 736–40. http://dx.doi.org/10.21273/jashs.128.5.0736.

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5S ribosomal DNA (rDNA) was visualized on the somatic metaphase chromosome of persimmon (Diospyros kaki) and ten wild Diospyros species by fluorescent in situ hybridization (FISH). The digoxigenin (DIG)-labeled 5S rDNA probe was hybridized onto the chromosomes and visualized by incubation with anti-DIG-fluorescein isothiocyanate (FITC). Strong signals of 5S rDNA probe were observed on several chromosomes of Diospyros species tested. Furthermore, multicolor FISH using 5S and 45S rDNA probes differently labeled with DIG and biotin, revealed separate localization of the two rDNA genes on differen
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Dissertations / Theses on the topic "45S ribosomal RNA genes (45S rDNA)"

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ARROYO, MARTINEZ HUGO ABELARDO 616509, and MARTINEZ HUGO ABELARDO ARROYO. "Determinación del cariotipo y mapeo físico de los genes 5s y 45s rDNA en Tigridia pavonia variedad Dulce." Tesis de maestría, Universidad Autónoma del Estado de México, 2016. http://hdl.handle.net/20.500.11799/65264.

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Tigridia pavonia (Lf) DC es una especie cuyas características morfológicas le confieren un gran potencial ornamental. Debido a la heterogeneidad morfológica de los individuos de esta especie, en el 2001 se describieron nueve variedades botánicas. Entre ellas Dulce presenta la menor formación de semillas lo cual podría significar la presencia de cambios cromosómicos. El establecimiento de las características citogenéticas de una especie es de gran interés para estudios taxonómicos y en programas de mejoramiento genético ya que facilita la selección de especies parentales más cercanas. Aunado a
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