Academic literature on the topic '45s rRNA'

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Journal articles on the topic "45s rRNA"

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Dyomin, Alexander, Svetlana Galkina, Arina Ilina, and Elena Gaginskaya. "Single Copies of the 5S rRNA Inserted into 45S rDNA Intergenic Spacers in the Genomes of Nototheniidae (Perciformes, Actinopterygii)." International Journal of Molecular Sciences 24, no. 8 (2023): 7376. http://dx.doi.org/10.3390/ijms24087376.

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In the vast majority of Animalia genomes, the 5S rRNA gene repeats are located on chromosomes outside of the 45S rDNA arrays of the nucleolar organiser (NOR). We analysed the genomic databases available and found that a 5S rDNA sequence is inserted into the intergenic spacer (IGS) between the 45S rDNA repeats in ten species of the family Nototheniidae (Perciformes, Actinopterigii). We call this sequence the NOR-5S rRNA gene. Along with Testudines and Crocodilia, this is the second case of a close association between four rRNA genes within one repetitive unit in deuterostomes. In both cases, NO
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Delorme-Hinoux, Valérie, Assane Mbodj, Sophie Brando, et al. "45S rDNA Diversity In Natura as One Step towards Ribosomal Heterogeneity in Arabidopsis thaliana." Plants 12, no. 14 (2023): 2722. http://dx.doi.org/10.3390/plants12142722.

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The keystone of ribosome biogenesis is the transcription of 45S rDNA. The Arabidopsis thaliana genome contains hundreds of 45S rDNA units; however, they are not all transcribed. Notably, 45S rDNA units contain insertions/deletions revealing the existence of heterogeneous rRNA genes and, likely, heterogeneous ribosomes for rRNAs. In order to obtain an overall picture of 45S rDNA diversity sustaining the synthesis of rRNAs and, subsequently, of ribosomes in natura, we took advantage of 320 new occurrences of Arabidopsis thaliana as a metapopulation named At66, sampled from 0 to 1900 m of altitud
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Arai, Taiga, Kensuke Ishiguro, Satoshi Kimura, Yuriko Sakaguchi, Takeo Suzuki, and Tsutomu Suzuki. "Single methylation of 23S rRNA triggers late steps of 50S ribosomal subunit assembly." Proceedings of the National Academy of Sciences 112, no. 34 (2015): E4707—E4716. http://dx.doi.org/10.1073/pnas.1506749112.

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Ribosome biogenesis requires multiple assembly factors. In Escherichia coli, deletion of RlmE, the methyltransferase responsible for the 2′-O-methyluridine modification at position 2552 (Um2552) in helix 92 of the 23S rRNA, results in slow growth and accumulation of the 45S particle. We demonstrate that the 45S particle that accumulates in ΔrlmE is a genuine precursor that can be assembled into the 50S subunit. Indeed, 50S formation from the 45S precursor could be promoted by RlmE-mediated Um2552 formation in vitro. Ribosomal protein L36 (encoded by rpmJ) was completely absent from the 45S pre
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Harrington, C. A., and D. M. Chikaraishi. "Transcription of spacer sequences flanking the rat 45S ribosomal DNA gene." Molecular and Cellular Biology 7, no. 1 (1987): 314–25. http://dx.doi.org/10.1128/mcb.7.1.314-325.1987.

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The transcriptional activity of spacer sequences flanking the rat 45S ribosomal DNA (rDNA) gene were studied. Nascent RNA labeled in in vitro nuclear run-on reactions hybridized with both 5' and 3' spacer regions. The highest level of hybridization was seen with an rDNA fragment containing tandem repeats of a 130-base-pair sequence upstream of the 45S rRNA initiation site. Synthesis of RNA transcripts homologous to this internally repetitious spacer region was insensitive to high levels of alpha-amanitin, suggesting that it is mediated by RNA polymerase I. Analysis of steady-state RNA showed t
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Harrington, C. A., and D. M. Chikaraishi. "Transcription of spacer sequences flanking the rat 45S ribosomal DNA gene." Molecular and Cellular Biology 7, no. 1 (1987): 314–25. http://dx.doi.org/10.1128/mcb.7.1.314.

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The transcriptional activity of spacer sequences flanking the rat 45S ribosomal DNA (rDNA) gene were studied. Nascent RNA labeled in in vitro nuclear run-on reactions hybridized with both 5' and 3' spacer regions. The highest level of hybridization was seen with an rDNA fragment containing tandem repeats of a 130-base-pair sequence upstream of the 45S rRNA initiation site. Synthesis of RNA transcripts homologous to this internally repetitious spacer region was insensitive to high levels of alpha-amanitin, suggesting that it is mediated by RNA polymerase I. Analysis of steady-state RNA showed t
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Dunn, Sianadh, Olivia Lombardi, and Victoria H. Cowling. "c-Myc co-ordinates mRNA cap methylation and ribosomal RNA production." Biochemical Journal 474, no. 3 (2017): 377–84. http://dx.doi.org/10.1042/bcj20160930.

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The mRNA cap is a structure added to RNA pol II transcripts in eukaryotes, which recruits factors involved in RNA processing, nuclear export and translation initiation. RNA guanine-7 methyltransferase (RNMT)–RNA-activating miniprotein (RAM), the mRNA cap methyltransferase complex, completes the basic functional mRNA cap structure, cap 0, by methylating the cap guanosine. Here, we report that RNMT–RAM co-ordinates mRNA processing with ribosome production. Suppression of RNMT–RAM reduces synthesis of the 45S ribosomal RNA (rRNA) precursor. RNMT–RAM is required for c-Myc expression, a major regul
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Rabanal, Fernando A., Viktoria Nizhynska, Terezie Mandáková, et al. "Unstable Inheritance of 45S rRNA Genes in Arabidopsis thaliana." G3: Genes|Genomes|Genetics 7, no. 4 (2017): 1201–9. http://dx.doi.org/10.1534/g3.117.040204.

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Stec, Michael J., David L. Mayhew, and Marcas M. Bamman. "The effects of age and resistance loading on skeletal muscle ribosome biogenesis." Journal of Applied Physiology 119, no. 8 (2015): 851–57. http://dx.doi.org/10.1152/japplphysiol.00489.2015.

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The hypertrophic response to resistance training is generally attenuated with aging; yet the mechanisms regulating this phenomenon are largely unknown. Several studies to date have shown blunted translational efficiency following acute resistance exercise in older adults; however, the effects on translational capacity (i.e., ribosome biogenesis) have not yet been examined. Thus the purpose of this study was to examine changes in markers of ribosome biogenesis following an acute bout of resistance loading (RL; 9 sets × 10 repetitions of knee extensions) in younger (Y; n = 14; 39.2 ± 4.1 yr) and
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Locati, Mauro D., Johanna F. B. Pagano, Farah Abdullah, et al. "Identifying small RNAs derived from maternal- and somatic-type rRNAs in zebrafish development." Genome 61, no. 5 (2018): 371–78. http://dx.doi.org/10.1139/gen-2017-0202.

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rRNAs are non-coding RNAs present in all prokaryotes and eukaryotes. In eukaryotes there are four rRNAs: 18S, 5.8S, 28S, originating from a common precursor (45S), and 5S. We have recently discovered the existence of two distinct developmental types of rRNA: a maternal-type, present in eggs and a somatic-type, expressed in adult tissues. Lately, next-generation sequencing has allowed the discovery of new small-RNAs deriving from longer non-coding RNAs, including small-RNAs from rRNAs (srRNAs). Here, we systemically investigated srRNAs of maternal- or somatic-type 18S, 5.8S, 28S, with small-RNA
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Sergeeva, Olga, Philipp Sergeev, Pavel Melnikov, Tatiana Prikazchikova, Olga Dontsova, and Timofei Zatsepin. "Modification of Adenosine196 by Mettl3 Methyltransferase in the 5’-External Transcribed Spacer of 47S Pre-rRNA Affects rRNA Maturation." Cells 9, no. 4 (2020): 1061. http://dx.doi.org/10.3390/cells9041061.

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Ribosome biogenesis is among the founding processes in the cell. During the first stages of ribosome biogenesis, polycistronic precursor of ribosomal RNA passes complex multistage maturation after transcription. Quality control of preribosomal RNA (pre-rRNA) processing is precisely regulated by non-ribosomal proteins and structural features of pre-rRNA molecules, including modified nucleotides. However, many participants of rRNA maturation are still unknown or poorly characterized. We report that RNA m6A methyltransferase Mettl3 interacts with the 5′ external transcribed spacer (5′ETS) of the
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Dissertations / Theses on the topic "45s rRNA"

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Kurata, Naoko. "Sea Surface Microlayer Microbial Observation System." NSUWorks, 2012. http://nsuworks.nova.edu/occ_stuetd/188.

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Chapter 2 The sea surface microlayer is a biogenic thin layer, comprising less than one millimeter of the ocean surface. This surface layer has gained much attention due to its dampening effect on ocean capillary ripples. The chemistry of the air-sea interface has been studied for decades; however, the structure and function of the marine bacterial community within the sea surface microlayer are still understudied. Although various sea surface microlayer sampling techniques were developed over the past decades, aseptic bacterial sampling in the open ocean is a rather challenging task. In this
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Ortiz-Ortiz, Marianyoly. "Kartchner Caverns: Habitat Scale Community Diversity and Function in a Carbonate Cave." Diss., The University of Arizona, 2012. http://hdl.handle.net/10150/265356.

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This dissertation examines the microbial and functional diversity in Kartchner Caverns, a limestone cave in Arizona, USA. Kartchner is highly oligotrophic due to the lack of photosynthesis and the limited inputs of organic material from the surface. This characteristic poses a challenge for microbial life in the cave. The first objective of this work was to evaluate the bacterial richness, diversity and taxonomic composition of speleothems surfaces within Kartchner Caverns in order to gain insight into the distribution patterns associated with these communities. Secondly, the metabolic str
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Patel, Jignasa. "Meta-Transcriptome Profiles of the Marine Sponge, Axinella corrugata and its Microbial Consortia: A Pyrosequencing Approach." NSUWorks, 2012. http://nsuworks.nova.edu/occ_stuetd/174.

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Marine micro-organisms are important components of various biogeochemical cycles, complex food webs and ecological niches. Metagenomic sequencing can provide rapid profile of metabolic activities within the sponge and resident microbes. However, the study of metatranscriptomes from sponges using high throughput sequencing technology has only recently begun. Through this study we isolated, characterized and compared metatranscriptome profiles of Axinella corrugata host and sponge-specific microbial communities using 454 pyrosequencing technology. Four cDNA libraries (two eukaryotic and two prok
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Raoelijaona, Raivoniaina. "Compréhension des rôles des complexes Nob1/Pno1 et RPS14/Cinap dans la maturation cytoplasmique de la petite sous-unité ribosomique (pré-40S) chez les eucaryotes." Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0221/document.

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Les ribosomes sont des complexes nucléoproétiques responsables de la traduction. Chez les eucaryotes, la biogenèse du ribosome est un processus complexe très régulé qui fait intervenir un nombre important de facteurs d’assemblages (~200). La construction d’un ribosome est initiée dans le nucléole puis continue dans le nucléoplasme et se termine dans le cytoplasme. La maturation cytoplasmique de la petite sous-unité ribosomale implique la dissociation séquentielle des facteurs d’assemblage tardifs et la maturation finale de l’ARNr 18S. Ce processus est catalysé par l’endonucléase Nob1 qui assur
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Walton, Charles. "Bacterial Communities Associated with Healthy and Diseased Acropora cervicornis (Staghorn Coral) Using High-Throughput Sequencing." NSUWorks, 2017. http://nsuworks.nova.edu/occ_stuetd/449.

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Coral diseases were first noted in the 1960s and 1970s and have had major impacts globally on coral reef community structures. In the Caribbean, a major outbreak of white band disease has been considered responsible for the drastic decline of Caribbean Acroporids since the 1970s. In addition to white band disease, another more recently described condition known as rapid tissue loss (RTL) has had major impacts on Acropora cervicornis populations, specifically offshore Broward County Southeast Florida. While these diseases have contributed to the population decline, determining their etiologies
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Dzidic, Majda. "Microbiota development and mucosal IgA responses during childhood in health and allergic disease." Doctoral thesis, Universitat Politècnica de València, 2019. http://hdl.handle.net/10251/125479.

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[ES] Antecedentes: Los patrones de colonización microbiana alterados durante la infancia pueden ser en parte responsables del aumento de enfermedades alérgicas en los países desarrollados. La microbiota intestinal difiere en composición y diversidad durante los primeros meses de vida en niños que luego desarrollan o no una enfermedad alérgica. Sin embargo, poco se sabe sobre la importancia de las respuestas inmunitarias tempranas de la mucosa a la microbiota intestinal en el desarrollo de alergias infantiles. Además, los estudios con respecto al efecto protector de la microbiota de la leche ma
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Pinder, Shaun. "Detecting Changes in the Gut Microbiome following Human Biotherapy via Pyrosequencing of the 16S rRNA Gene." Thesis, 2013. http://hdl.handle.net/10214/6578.

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Human biotherapy (HBT) or fecal transplants have been shown to be an effective treatment for patients with recurrent Clostridium difficile infection (CDI). This study examines the microbial populations present in CDI patients pre- and post-HBT by extracting bacterial DNA from stool samples and performing pyrosequencing of the 16S rRNA gene. We then compared these microbial populations to those of the donors. We examined 19 pairs of patient samples, of which 14 were clinically cured of CDI, and 5 patients were failures. The successful treatment of CDI was associated with an increase in diversit
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Hu, Ping. "Effects of Oilseed Meals and Isothiocyanates (ITCS) on Phymatotrichopsis omnivora (Cotton Root Rot) and Soil Microbial Communities." Thesis, 2012. http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-10932.

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The meals from many oilseed crops contain biocidal chemicals that are known to inhibit the growth and activity of several soil pathogens, though little is known concerning impacts on whole soil microbial communities. We investigated the effect of oilseed meals (SMs) from both brassicaceous plants, including mustard and camelina, as well as non-brassicaceous plants, including jatropha and flax, on P. omnivora (the casual agent of cotton root rot) in Branyon clay soil (at 1 and 5% application rates). We also investigated the effect of SMs from camelina, jatropha, flax, and wheat straw on microbi
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Dukunde, Amélie. "Phylogenetic and functional diversity of soil prokaryotic communities in temperate deciduous forests with different tree species." Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E61A-A.

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Peters, Fabian. "Entwicklung von Lanthanoid-Tags für die biomolekulare NMR-Spektroskopie." Doctoral thesis, 2010. http://hdl.handle.net/11858/00-1735-0000-0006-B095-9.

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Book chapters on the topic "45s rRNA"

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Rabanal, Fernando A. "Characterization of the Different Levels of Variation in 45S rRNA Genes." In Plant Gametogenesis. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2253-7_24.

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Remonsellez, F., F. Galleguillos, Sonestie Janse van Rensburg, et al. "Monitoring of Microbial Community Inhabiting a Low-Grade Copper Sulphide Ore by Quantitative Real-Time PCR Analysis of 16S rRNA Genes." In Advanced Materials Research. Trans Tech Publications Ltd., 2007. http://dx.doi.org/10.4028/0-87849-452-9.539.

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Ndung’u Mwangi, Harrison, and Francis Jackim Mulaa. "rRNA Platform Technology for Drug Discovery Methods for Identifying Ligands That Target Plasmodium RNA Structural Motifs." In Plasmodium Species and Drug Resistance. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.98776.

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Determining the structure of the P. falciparum40s leads to better understanding of the structural basis for its protein-synthesizing roles in the cell. This enables researchers in the field of drug development to run in silico ligand screening experiments using the solved P. falciparum 40S structure as a target against a library of potential anti-malarial compounds. Drug leads identified through this method can lead to further biochemical and In vitro binding studies with the ultimate goal of developing new class of anti-malarial drugs. The use of structure prediction and modeling technologies
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Sánchez, Olga, Isabel Ferrera, Jose González, and Jordi Mas. "Assessing Bacterial Diversity in a Seawater-processing Wastewater Treatment Plant by 454-pyrosequencing of the16S rRNA and amoA Genes." In Environmental Engineering and Activated Sludge Processes. Apple Academic Press, 2016. http://dx.doi.org/10.1201/b19902-10.

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Brøndsted, Lone, and Karin Hammer. "Phages of Lactococcus lactis." In The Bacteriophages. Oxford University PressNew York, NY, 2005. http://dx.doi.org/10.1093/oso/9780195148503.003.0036.

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Abstract Lactococci are Gram-positive mesophilic bacteria with low G-C content that belong to the group of lactic acid bacteria. They are aerotolerant and live by means of fermentation, as they are lacking a respiratory chain, and the main end product during fermentation is lactic acid. In nature lactococci are mainly found on plant material (100). Some lactococci have apparently adapted to multiply in milk and contain a plasmid-encoded milk protease and enzymes responsible for catabolism of lactose. Lactococcus lactis is used world-wide as starter culture for large-scale milk fermentations pr
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"Assessing Bacterial Diversity in a Seawater-processing Wastewater Treatment Plant by 454-pyrosequencing of the 16S rRNA and amoA Genes." In Environmental Engineering and Activated Sludge Processes. Apple Academic Press, 2016. http://dx.doi.org/10.1201/b19902-13.

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"- Assessing Bacterial Diversity in a Seawater-Processing Wastewater Treatment Plant by 454-Pyrosequencing of the 16S rRNA and amoA Genes." In Bioremediation of Wastewater. Apple Academic Press, 2016. http://dx.doi.org/10.1201/b18618-11.

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Conference papers on the topic "45s rRNA"

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Alabbas, Faisal M., Anthony E. Kakpovbia, John R. Spear, Brajendra Mishra, and David L. Olson. "Utilization of 454 Pyrosequencing of 16S rRNA for Biodiversity Investigations of Crude Oil Systems." In CORROSION 2014. NACE International, 2014. https://doi.org/10.5006/c2014-3827.

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Abstract Microbiologically influenced corrosion (MIC) is a major problem that impacts crude oil production, transportation and storage infrastructures. Indigenous microorganisms that naturally reside in oil reservoirs are able to induce localized changes in the aqueous environment and lead to catastrophic damages. The study herein applies molecular techniques to investigate the microbial communities associated with corrosion products collected from crude oil pipelines. Small subunit ribosomal rRNA gene pyrosequencing was used to identify microbial communities present in each system. The result
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