Relevant bibliographies by topics / 5' mRNA end

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers
  5. Reports

Academic literature on the topic '5' mRNA end'

Author: Grafiati
Published: 24 April 2022

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Journal articles on the topic "5' mRNA end"

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Chiang, Chiayn, Guang-Wu Chen, and Shin-Ru Shih. "Mutations at Alternative 5′ Splice Sites of M1 mRNA Negatively Affect Influenza A Virus Viability and Growth Rate." Journal of Virology 82, no. 21 (2008): 10873–86. http://dx.doi.org/10.1128/jvi.00506-08.

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ABSTRACT Different amino acid sequences of influenza virus proteins contribute to different viral phenotypes. However, the diversity of the sequences and its impact on noncoding regions or splice sites have not been intensively studied. This study focuses on the sequences at alternative 5′ splice sites on M1 mRNA. Six different mutations at the splice sites were introduced, and viral growth characteristics for those mutants generated by reverse genetics with 12 plasmids were examined, for which G12C (the G-to-C mutation at the first nucleotide of the intron for the mRNA3 5′ splice site), C51G
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Zhai, Li-ting, and Song Xiang. "mRNA quality control at the 5’ end." Journal of Zhejiang University SCIENCE B 15, no. 5 (2014): 438–43. http://dx.doi.org/10.1631/jzus.b1400070.

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Cheng, Hong, Kobina Dufu, Chung-Sheng Lee, Jeanne L. Hsu, Anusha Dias, and Robin Reed. "Human mRNA Export Machinery Recruited to the 5′ End of mRNA." Cell 127, no. 7 (2006): 1389–400. http://dx.doi.org/10.1016/j.cell.2006.10.044.

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Kowalska, Joanna, Maciej Lukaszewicz, Joanna Zuberek, Edward Darzynkiewicz, and Jacek Jemielity. "Phosphoroselenoate Dinucleotides for Modification of mRNA 5′ End." ChemBioChem 10, no. 15 (2009): 2469–73. http://dx.doi.org/10.1002/cbic.200900522.

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Liu, H., and M. Kiledjian. "Decapping the message: a beginning or an end." Biochemical Society Transactions 34, no. 1 (2006): 35–38. http://dx.doi.org/10.1042/bst0340035.

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Removal of the mRNA 5′ cap is an important step in the regulation of mRNA stability. mRNAs are degraded by at least two distinct exonucleolytic decay pathways, one from the 5′ end, and the second from the 3′ end. Two major cellular decapping enzymes have been identified, and each primarily functions in one of the two decay pathways. The Dcp2 decapping enzyme utilizes capped mRNA as substrate and hydrolyses the cap to release m7GDP (N7-methyl GDP), while a scavenger decapping enzyme, DcpS, utilizes cap dinucleotides or capped oligonucleotides as substrate and releases m7GMP (N7-methyl GMP). In
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Ahsan, B., T. L. Saito, S. i. Hashimoto, et al. "MachiBase: a Drosophila melanogaster 5'-end mRNA transcription database." Nucleic Acids Research 37, Database (2009): D49—D53. http://dx.doi.org/10.1093/nar/gkn694.

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Escors, David, Ander Izeta, Carmen Capiscol, and Luis Enjuanes. "Transmissible Gastroenteritis Coronavirus Packaging Signal Is Located at the 5′ End of the Virus Genome." Journal of Virology 77, no. 14 (2003): 7890–902. http://dx.doi.org/10.1128/jvi.77.14.7890-7902.2003.

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ABSTRACT To locate the transmissible gastroenteritis coronavirus (TGEV) packaging signal, the incorporation of TGEV subgenomic mRNAs (sgmRNAs) into virions was first addressed. TGEV virions were purified by three different techniques, including an immunopurification using an M protein-specific monoclonal antibody. Detection of sgmRNAs in virions by specific reverse transcription-PCRs (RT-PCRs) was related to the purity of virus preparations. Interestingly, virus mRNAs were detected in partially purified virus but not in virus immunopurified using stringent conditions. Analyses by quantitative
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Li, You, Man-Gen Song, and Megerditch Kiledjian. "Transcript-Specific Decapping and Regulated Stability by the Human Dcp2 Decapping Protein." Molecular and Cellular Biology 28, no. 3 (2007): 939–48. http://dx.doi.org/10.1128/mcb.01727-07.

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ABSTRACT mRNA decapping is a critical step in the control of mRNA stability and gene expression and is carried out by the Dcp2 decapping enzyme. Dcp2 is an RNA binding protein that must bind RNA in order to recognize the cap for hydrolysis. We demonstrate that human Dcp2 (hDcp2) preferentially binds to a subset of mRNAs and identify sequences at the 5′ terminus of the mRNA encoding Rrp41, a core subunit component of the RNA exosome, as a specific hDcp2 substrate. A 60-nucleotide element at the 5′ end of Rrp41 mRNA was identified and shown to confer more efficient decapping on a heterologous RN
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Stefanovic, B., C. Hellerbrand та D. A. Brenner. "Regulatory Role of the Conserved Stem-Loop Structure at the 5′ End of Collagen α1(I) mRNA". Molecular and Cellular Biology 19, № 6 (1999): 4334–42. http://dx.doi.org/10.1128/mcb.19.6.4334.

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ABSTRACT Three fibrillar collagen mRNAs, α1(I), α2(I), and α1(III), are coordinately upregulated in the activated hepatic stellate cell (hsc) in liver fibrosis. These three mRNAs contain sequences surrounding the start codon that can be folded into a stem-loop structure. We investigated the role of this stem-loop structure in expression of collagen α1(I) reporter mRNAs in hsc’s and fibroblasts. The stem-loop dramatically decreases accumulation of mRNAs in quiescent hsc’s and to a lesser extent in activated hsc’s and fibroblasts. The stem-loop decreases mRNA stability in fibroblasts. In activat
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Liu, Hudan, and Megerditch Kiledjian. "Scavenger Decapping Activity Facilitates 5′ to 3′ mRNA Decay." Molecular and Cellular Biology 25, no. 22 (2005): 9764–72. http://dx.doi.org/10.1128/mcb.25.22.9764-9772.2005.

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ABSTRACT mRNA degradation occurs through distinct pathways, one primarily from the 5′ end of the mRNA and the second from the 3′ end. Decay from the 3′ end generates the m7GpppN cap dinucleotide, which is subsequently hydrolyzed to m7Gp and ppN in Saccharomyces cerevisiae by a scavenger decapping activity termed Dcs1p. Although Dcs1p functions in the last step of mRNA turnover, we demonstrate that its activity modulates earlier steps of mRNA decay. Disruption of the DCS1 gene manifests a threefold increase of the TIF51A mRNA half-life. Interestingly, the hydrolytic activity of Dcs1p was essent
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Dissertations / Theses on the topic "5' mRNA end"

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Naas, Adrian Ertsås. "Optimzing the 5'-end of Coding Sequences in Recombinant mRNA to achieve high-level Expression in the Bacterium Escherichia coli." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for bioteknologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-18368.

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Recombinant protein production in Escherichia coli provides a cheap and efficient way of producing medically and industrially relevant proteins. Sequence features of individual genes and especially their 5’ terminal coding sequences act on the efficiency of gene expression by complex regulatory mechanisms which are still not fully understood. This study aimed to investigate the features of the 5’ coding region of recombinant mRNAs, and to optimize them for increased expression in E. coli. A previous study had found that a synonymous change of the bla reporter gene 2nd codon leads to
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Gulseren, Cakmakci Nihal Marzluff William F. "Histone mRNA translation in metazoans SLIP1 as the bridging factor between the 5' and 3' UTRs of the histone mRNA /." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2007. http://dc.lib.unc.edu/u?/etd,813.

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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2007.<br>Title from electronic title page (viewed Dec. 18, 2007). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Biology." Discipline: Biology; Department/School: Biology.
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Chao, Ping. "Molecular basis of PKR activation by Interferon-gamma (IFNγ) mRNA 5’-UTR". Thesis, University of Manchester, 2011. https://www.research.manchester.ac.uk/portal/en/theses/molecular-basis-of-pkr-activation-by-interferongamma-ifn-mrna-5utr(76530b0e-e02f-4d59-8d38-4a2ba46fe741).html.

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PKR is an interferon (IFN)-induced protein kinase that is activated by double-stranded RNA in a mechanism involving binding of the N-terminal domain of PKR, protein dimerization and autophosphorylation. PKR is a key component of the cellular antiviral response and plays critical roles in a variety of other cellular processes including signal transduction, growth and apoptosis. Once activated, PKR phosphorylates its substrate, translation initiation factor (eIF2) on its alpha subunit at Ser51, thereby leading to an inhibition of protein synthesis. The IFN-gamma mRNA 5’-UTR, an H-type pesudoknot
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Pietrow, Paulina. "Synteza podwójnie funkcjonalizowanych dinukleotydowych analogów końca 5’ mRNA (tzw. kapu)." Doctoral thesis, 2020. https://depotuw.ceon.pl/handle/item/3737.

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Informacyjny kwas rybonukleinowy organizmów eukariotycznych i niektórych wirusów posiada na swoim końcu 5’ unikalną strukturę zwaną kapem. Powstaje ona na wczesnym etapie transkrypcji i uczestniczy w wielu ważnych procesach takich jak splicing, poliadenylacja czy transport do cytoplazmy, gdzie pośredniczy we włączaniu mRNA w proces translacji. Kap przyczynia się również do ochrony przed degradacją enzymatyczną eukariotycznego mRNA. Analogi końca 5’ mRNA z uwagi na ich potencjalne zastosowanie terapeutyczne od lat poddaje się różnorakim modyfikacjom wpływającym na pełnione przez kap funkcje np.
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Kasprzyk, Renata. "Metody badania białek związanych z metabolizmem końca 5' mRNA oparte na nukleotydowych sondach molekularnych." Doctoral thesis, 2021. https://depotuw.ceon.pl/handle/item/3944.

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Renata Kasprzyk STRESZCZENIE ROZPRAWY DOKTORSKIEJ Metody badania białek związanych z metabolizmem końca 5’ mRNA oparte na nukleotydowych sondach molekularnych Informacyjny RNA (mRNA) jest makrocząsteczką reprezentującą kopię informacji genetycznej, która ulega translacji w celu produkcji białka. Koniec 5’ mRNA, tzw. kap jest nietypową strukturą nukleotydową zbudowaną z 7-metyloguanozyny połączonej mostkiem 5’-5’ trifosforanowym z pierwszym transkrybowanym nukleotydem. Ze względu na obecność łańcucha dodatniego w pierścieniu 7-metyloguanozyny kap specyficznie oddziałuje z wieloma białkami zaang
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Wojtczak, Anna. "Wykorzystanie zmian polaryzacji fluorescencji oraz innych metod spektrofluorymetrycznych do badania aktywności oraz inhibicji białek oddziałujących z końcem 5’ mRNA." Doctoral thesis, 2021. https://depotuw.ceon.pl/handle/item/4064.

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Struktura kapu (m7GpppN) znajdująca się na końcu 5’ mRNA poprzez specyficzne oddziaływanie z białkami wiążącymi kap warunkuje procesy składające się na ekspresję genów. Nieprawidłowa aktywność białek wiążących kap może prowadzić do zaburzeń ekspresji genów, a w konsekwencji do chorób nowotworowych lub neurodegeneracyjnych. Regulowanie aktywności białek wiążących kap może następować poprzez dostarczanie małocząsteczkowych ligandów (analogów kapu), które będą wiązać się z białkami, jednocześnie uniemożliwiając ich wiązanie z końcem 5’ mRNA. Z uwagi na potencjalne znaczenie terapeutyczne ligandów
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Ciechanowicz, Sylwia. "Analogi kapu zawierające ugrupowanie triazolowe w łańcuchu fosforanowym - synteza, wbudowanie do RNA oraz badania wpływu modyfikacji na funkcje końca 5'mRNA." Doctoral thesis, 2019. https://depotuw.ceon.pl/handle/item/3245.

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The structure of 5’ mRNA end, so called cap, participates in most important processes of RNA metabolism, mainly by interacting with proteins and protecting mRNA from premature degradation. Because of its crucial biological role it became the object of chemical modifications aimed at obtaining useful tools for selective modulation of cap-dependent processes. Particularly desired effect of such modifications is the enhancement of affinity to translation initiation factor, eIF4E, as its interaction with the structure of 5’ mRNA end is the prerequisite of the initiation of protein biosynthesis. Fo
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Daniel, Donald L. "A tail of two ends 5 ́and 3 ́modifications of mRNAs and their functional significance /." 1997. http://catalog.hathitrust.org/api/volumes/oclc/37571577.html.

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Gupton, Leodis Darren. "Differential uncoupling of 5' and 3' exonucleolytic activities as determined by mutational analysis of the Saccharomyces cerevisiae exoribonuclease, RAT1." Thesis, 2010. http://hdl.handle.net/2152/ETD-UT-2010-05-1058.

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Eukaryotic gene expression requires hundreds of proteins and several RNA factors to facilitate nuclear RNA processing. These RNA processing events include RNA transcription, pre-mRNA splicing, pre-ribosomal RNA (pre-rRNA) processing and trafficking of RNA to its proper location in the cell. As we learn more about the molecular details of the factors governing these highly coordinated processes it is becoming increasingly clear that a subset of factors participate in multiple RNA processing pathways to ensure faithful gene expression. Our work completes the characterization of the Abelson pr
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Book chapters on the topic "5' mRNA end"

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Bacher, M., P. Hofmann, and D. Gemsa. "Resolution of a Secondary Structure in an Unknown mRNA 5’ End." In Methods in DNA Amplification. Springer US, 1994. http://dx.doi.org/10.1007/978-1-4615-2530-1_8.

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Marzluff, Williams F., Zbigniew Dominski, Michael L. Whitfield, and Zeng-Feng Wang. "Identification of the Protein That Interacts with the 3′ End of Histone mRNA." In mRNA Formation and Function. Elsevier, 1997. http://dx.doi.org/10.1016/b978-012587545-5/50011-2.

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Komarova, Anastassia V., Michèle Brocard, and Katherine M. Kean. "The Case for mRNA 5′ and 3′ End Cross Talk During Translation in a Eukaryotic Cell." In Progress in Nucleic Acid Research and Molecular Biology. Elsevier, 2006. http://dx.doi.org/10.1016/s0079-6603(06)81009-3.

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Li, Bingnan, Sueli Marques, Jingwen Wang, and Vicent Pelechano. "Using TIF-Seq2 to investigate association between 5´ and 3´mRNA ends." In Methods in Enzymology. Elsevier, 2021. http://dx.doi.org/10.1016/bs.mie.2021.03.017.

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GEIGER, MARY JANE, MICHAEL BULL, DAVID D. ECKELS, and JACK GORSKI. "Amplification of Complementary DNA from mRNA with Unknown 5′ Ends by One-Way Polymerase Chain Reaction." In Recombinant DNA Methodology II. Elsevier, 1995. http://dx.doi.org/10.1016/b978-0-12-765561-1.50047-3.

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Stevens, Adam, Shahin Tajbakhsh, Andrew Whatmore, Melissa Westwood, and Peter Clayton. "The Correlation of Clinical Response to Growth Hormone with mRNA Expression Profiles in Turner Syndrome." In TRANSLATIONAL - Pediatric Endocrinology: Growth & Adrenal. The Endocrine Society, 2011. http://dx.doi.org/10.1210/endo-meetings.2011.part4.or14.or42-5.

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Geiger, Maryjane, Michael Bull, David D. Eckels, and Jack Gorski. "[22] Amplification of complementary DNA from mRNA with unknown 5′ ends by one-way polymerase chain reaction." In Methods in Enzymology. Elsevier, 1993. http://dx.doi.org/10.1016/0076-6879(93)18024-7.

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Conference papers on the topic "5' mRNA end"

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Jemielity, Jacek, Maciej Lukaszewicz, Joanna Kowalska, Jakub Czarnecki, Joanna Zuberek, and Edward Darżynkiewicz. "Synthesis and properties of dinucleotide cap analog for mRNA 5' end labeling." In XVth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2011. http://dx.doi.org/10.1135/css201112351.

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Cool, D. E., and R. T. A. MacGillivray. "CHARACTERIZATION OF THe HUMAN FACTOR XII GENE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642800.

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Surface activation of the plasma systems involved with coagulation, fibrinolysis, renin formation and kinin generation involves factor XII (Hageman factor). This protein is a 76,000 dalton glycoprotein which circulates in plasma as an inactive form of a serine protease. A human liver cDNA coding for factor XII was used to screen a human genomic phage library. Two overlapping clones were isolated, XHXII27 and XHXII76, and contain the entire gene for human factor XII. The gene is 13.5 Kbp in length and consists of 14 exons and 13 introns. The transcriptional start site of the mRNA was determined
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Beeler, D., L. Fritze, G. Soff, R. Jackman, and R. Rosenberg. "HUMAN THROMBOMODULIN cDNA:SEQUENCE AND TRANSLATED STRUCTURE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643967.

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A 750 bp bovine Thrombomodulin (TM) cDNA fragment was used as an hybridization probe to screen an oligo-dT primed Lambda gtll. cDNA library prepared from human umbilical vein endothelial cell mRNA. A 2.4 kb positive human clone was isolated which showed an 80% nucleotide sequence homology with bovine TM cDNA. This clone and a 550 bp fragment from its 5' end were used to further screen the oligo-dT primed library as well as randomly primed library prepared from the same mRNA. The cDNA clones obtained allow us to describe the overall structure of human TM and reveal that it is extremely similar
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MORENO-SOSA, María Tamara, Daiana Sthefania GARCIA, Rocío HEREDIA, Victor BITTAR-RIVERO-PEDROSA, and Juan Pablo MACKERN-OBERTI. "PRL-R ISOFORMS LONG / SHORT RATIO IS INCREASED IN SYSTEMIC LUPUS ERYTHEMATOSUS BLOOD MONONUCLEAR CELLS." In SOUTHERN BRAZILIAN JOURNAL OF CHEMISTRY 2021 INTERNATIONAL VIRTUAL CONFERENCE. DR. D. SCIENTIFIC CONSULTING, 2022. http://dx.doi.org/10.48141/sbjchem.21scon.21_abstract_mackern.pdf.

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Prolactin (PRL) displays several functions in the whole body by binding its receptor (PRL-R). Two main PRL-R isoforms are reported that differ in their capacity to trigger signaling pathways, PRL-R long isoform is the activation receptor, and the short isoform is the inhibitory oner. Although many autoimmune diseases display hyperprolactinemia, the role of each PRL-R isoform expression in autoimmunity remains unknown. This work aimed to correlate PRL-R isoforms expression in human peripheral blood mononuclear cells (PBMCs) of female Systemic Lupus Erythematosus patients and healthy controls. T
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El-fadl, Rihab, Nasser Rizk, Amena Fadel, and Abdelrahman El Gamal. "The Profile of Hepatic Gene Expression of Glucose Metabolism in Mice on High Fat Diet." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0213.

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Obesity is a growing problem worldwide, and recent data indicated that 20% of the populations would be obese. Obesity arises as a multifactorial disease caused by inherited traits that interact with lifestyle factors such as diet and physical activity. The liver plays an essential role in the gluco-regulation via regulating glucose, lipid and protein metabolism. The process of glucose metabolism is controlled by a range of molecular mechanisms and genes which affect the metabolism of the liver during intake of high fat diet (HFD). The objective of this research is to investigate the profile of
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Singh, Harinder J., Young-Tai Choi, and Norman M. Wereley. "Optimal Control of Vertically Stroking Crew Seats Employing Magnetorheological Energy Absorbers." In ASME 2009 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. ASMEDC, 2009. http://dx.doi.org/10.1115/smasis2009-1361.

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Nondimensional analyses of vertical stroking crew seats with adaptive nonlinear magnetorheological energy absorbers (MREA) and magnetorheological shock isolation (MRSI) were addressed in this study. Under consideration were single-degree-of-freedom vertically stroking seat systems consisting of a rigid occupant mass falling with prescribed initial impact velocity (sink rate). The governing equations of the vertical stroking crew seats were derived using nondimensional variables such as nondimensional stroke, velocity, acceleration and time constant, as well as nondimensional Bingham number (i.
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Pannekoek, H., M. Linders, J. Keijer, H. Veerman, H. Van Heerikhuizen, and D. J. Loskutoff. "THE STRUCTURE OF THE HUMAN ENDOTHELIAL PLASMINOGEN ACTIVATOR INHIBITOR (PAI-1) GENE: NON-RANDOM POSITIONING OF INTRONS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644767.

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The endothelium plays a crucial role in the regulation of the fibrinolytic process, since it synthesizes and secretes tissue-type plasminogen activator (t-PA) as well as the fast-acting plasminogen activator inhibitor (PAI-1). Molecular cloning of full-length PAI-1 cDNA, employing a human endothelial cDNA expression library, and a subsequent determination of the complete nucleotide sequence, allowed a prediction of the amino-acid sequence of the PAI-1 glycoprotein. It was observed that the amino-acid sequence is significantly homologous to those of members of the serine protease inhibitor ("Se
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Reports on the topic "5' mRNA end"

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Funkenstein, Bruria, and Cunming Duan. GH-IGF Axis in Sparus aurata: Possible Applications to Genetic Selection. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7580665.bard.

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Many factors affect growth rate in fish: environmental, nutritional, genetics and endogenous (physiological) factors. Endogenous control of growth is very complex and many hormone systems are involved. Nevertheless, it is well accepted that growth hormone (GH) plays a major role in stimulating somatic growth. Although it is now clear that most, if not all, components of the GH-IGF axis exist in fish, we are still far from understanding how fish grow. In our project we used as the experimental system a marine fish, the gilthead sea bream (Sparus aurata), which inhabits lagoons along the Mediter
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Aharoni, Asaph, Zhangjun Fei, Efraim Lewinsohn, Arthur Schaffer, and Yaakov Tadmor. System Approach to Understanding the Metabolic Diversity in Melon. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7593400.bard.

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Fruit quality is determined by numerous genetic factors that affect taste, aroma, ‎color, texture, nutritional value and shelf life. To unravel the genetic components ‎involved in the metabolic pathways behind these traits, the major goal of the project was to identify novel genes that are involved in, or that regulate, these pathways using correlation analysis between genotype, metabolite and gene expression data. The original and specific research objectives were: (1) Collection of replicated fruit from a population of 96 RI lines derived from parents distinguished by great diversity in frui
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