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1
Hu, Ming Ming, and Shu Xu. "Discussion on the Contents of 5S Activities in Universities." Advanced Materials Research 219-220 (March 2011): 1427–30. http://dx.doi.org/10.4028/www.scientific.net/amr.219-220.1427.
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Regarding the shortcomings of 5S activities mainly carried out by local in universities, this article puts forward the idea of the full implementation of 5S activities within the universities. From the classroom 5S, dormitories 5S, library 5S, laboratory 5S, etc, we made researches on the contents of 5S activities in universities, which can provide a better reference for the implementation of 5S activities in universities.
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Kmiec, E. B., and A. Worcel. "The positive transcription factor of the 5S RNA gene proteolyses during direct exchange between 5S DNA sites." Journal of Cell Biology 103, no. 3 (September 1, 1986): 673–81. http://dx.doi.org/10.1083/jcb.103.3.673.
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We have examined the association, dissociation, and exchange of the 5S specific transcription factor (TFIIIA) with somatic- and oocyte-type 5S DNA. The factor associates faster with somatic than with oocyte 5S DNA, and the rate of complex formation is accelerated by vector DNA. Once formed, the TFIIIA-5S DNA complex is stable for greater than 4 h in the absence of free 5S DNA, and its dissociation is identical for somatic and for oocyte 5S DNA. In the presence of free 5S DNA, the factor transfers promptly from the complex to the free 5S DNA site. Unexpectedly, the direct exchange of factor between 5S DNA sites leads to proteolysis at the C-terminal arm of TFIIIA.
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Randhawa, Jugraj Singh, and Inderpreet Singh Ahuja. "5S – a quality improvement tool for sustainable performance: literature review and directions." International Journal of Quality & Reliability Management 34, no. 3 (March 6, 2017): 334–61. http://dx.doi.org/10.1108/ijqrm-03-2015-0045.
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Purpose The purpose of this paper is to present the comprehensive literature review on 5S and suggest possible gaps from the point of view of researchers and practitioners. The manuscript presents the overview of 5S implementation and highlights the achievements realized from deployment of 5S initiatives for sustainable performance of organizations. Design/methodology/approach The paper critically examines the literature on 5S, analyzes and reviews it systematically. The study highlights the critical barriers and success factors for sustainable 5S implementation in the organizations in the competitive world. Findings 5S is an outstanding Japanese philosophy for the development of any type organization all over the world. This study bring out the concept of 5S, requirements for its holistic implementation, relationship with other lean tools, benefits, success factors and obstacles in 5S implementation. The significant contributions through 5S initiatives in the organization like production, quality, safety and effective utilization of workspace for the sustained organizational improvement have also been highlighted in the study. Practical implications The literature on assortment of 5S technique has been so far very limited. The present paper reviews large number of research publications related to 5S to highlight the significance of 5S philosophy in the sustainable organizational improvement across the world. It foregrounds the approach advised by the various researchers, practitioners and appraises censoriously the reason behind the demand of 5S program in the organization. The needful steps and obstacles are also foreground for the effective implementation of 5S in the organization. Originality/value The paper presents a comprehensive review of literature publications in the area of 5S and their assortment to develop an understanding of the significance and implementation of 5S in the organizations. The paper will be helpful or useful to researchers, safety executives, development professionals and managers in the organizations.
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Chipev, C. C., and A. P. Wolffe. "Chromosomal organization of Xenopus laevis oocyte and somatic 5S rRNA genes in vivo." Molecular and Cellular Biology 12, no. 1 (January 1992): 45–55. http://dx.doi.org/10.1128/mcb.12.1.45-55.1992.
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We describe the chromosomal organization of the major oocyte and somatic 5S RNA genes of Xenopus laevis in chromatin isolated from erythrocyte nuclei. Both major oocyte and somatic 5S DNA repeats are associated with nucleosomes; however, differences exist in the organization of chromatin over the oocyte and somatic 5S RNA genes. The repressed oocyte 5S RNA gene is protected from nuclease digestion by incorporation into a nucleosome, and the entire oocyte 5S DNA repeat is assembled into a loosely positioned array of nucleosomes. In contrast, the potentially active somatic 5S RNA gene is accessible to nuclease digestion, and the majority of somatic 5S RNA genes appear not to be incorporated into positioned nucleosomes. Evidence is presented supporting the stable association of transcription factors with the somatic 5S RNA genes. Histone H1 is shown to have a role both in determining the organization of nucleosomes over the oocyte 5S DNA repeat and in repressing transcription of the oocyte 5S RNA genes.
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Chipev, C. C., and A. P. Wolffe. "Chromosomal organization of Xenopus laevis oocyte and somatic 5S rRNA genes in vivo." Molecular and Cellular Biology 12, no. 1 (January 1992): 45–55. http://dx.doi.org/10.1128/mcb.12.1.45.
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We describe the chromosomal organization of the major oocyte and somatic 5S RNA genes of Xenopus laevis in chromatin isolated from erythrocyte nuclei. Both major oocyte and somatic 5S DNA repeats are associated with nucleosomes; however, differences exist in the organization of chromatin over the oocyte and somatic 5S RNA genes. The repressed oocyte 5S RNA gene is protected from nuclease digestion by incorporation into a nucleosome, and the entire oocyte 5S DNA repeat is assembled into a loosely positioned array of nucleosomes. In contrast, the potentially active somatic 5S RNA gene is accessible to nuclease digestion, and the majority of somatic 5S RNA genes appear not to be incorporated into positioned nucleosomes. Evidence is presented supporting the stable association of transcription factors with the somatic 5S RNA genes. Histone H1 is shown to have a role both in determining the organization of nucleosomes over the oocyte 5S DNA repeat and in repressing transcription of the oocyte 5S RNA genes.
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Bogenhagen, D. F. "Proteolytic footprinting of transcription factor TFIIIA reveals different tightly binding sites for 5S RNA and 5S DNA." Molecular and Cellular Biology 13, no. 9 (September 1993): 5149–58. http://dx.doi.org/10.1128/mcb.13.9.5149-5158.1993.
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Transcription factor IIIA (TFIIIA) employs an array of nine N-terminal zinc fingers to bind specifically to both 5S RNA and 5S DNA. The binding of TFIIIA to 5S RNA and 5S DNA was studied by using a protease footprinting technique. Brief treatment of free or bound TFIIA with trypsin or chymotrypsin generated fragments which were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Fragments retaining the N terminus of TFIIA were identified by immunoblotting with an antibody directed against the N terminus of TFIIIA. Proteolytic footprinting of TFIIIA complexed with 5S DNA derivatives reinforced other evidence that the three N-terminal zinc fingers of TFIIIA bind most tightly to 5S DNA. Proteolytic footprinting of TFIIIA in reconstituted 7S ribonucleoprotein particles revealed different patterns of trypsin sensitivity for TFIIIA bound to oocyte versus somatic 5S RNA. Trypsin cleaved TFIIIA between zinc fingers 3 and 4 more readily when the protein was bound to somatic 5S RNA than when it was bound to oocyte 5S RNA. A tryptic fragment of TFIIIA containing zinc fingers 4 through 7 remained tightly associated with somatic 5S RNA. Zinc fingers 4 through 7 may represent a tightly binding site for 5S RNA in the same sense that fingers 1 through 3 represent a tightly binding site for 5S DNA.
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Bogenhagen, D. F. "Proteolytic footprinting of transcription factor TFIIIA reveals different tightly binding sites for 5S RNA and 5S DNA." Molecular and Cellular Biology 13, no. 9 (September 1993): 5149–58. http://dx.doi.org/10.1128/mcb.13.9.5149.
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Transcription factor IIIA (TFIIIA) employs an array of nine N-terminal zinc fingers to bind specifically to both 5S RNA and 5S DNA. The binding of TFIIIA to 5S RNA and 5S DNA was studied by using a protease footprinting technique. Brief treatment of free or bound TFIIA with trypsin or chymotrypsin generated fragments which were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Fragments retaining the N terminus of TFIIA were identified by immunoblotting with an antibody directed against the N terminus of TFIIIA. Proteolytic footprinting of TFIIIA complexed with 5S DNA derivatives reinforced other evidence that the three N-terminal zinc fingers of TFIIIA bind most tightly to 5S DNA. Proteolytic footprinting of TFIIIA in reconstituted 7S ribonucleoprotein particles revealed different patterns of trypsin sensitivity for TFIIIA bound to oocyte versus somatic 5S RNA. Trypsin cleaved TFIIIA between zinc fingers 3 and 4 more readily when the protein was bound to somatic 5S RNA than when it was bound to oocyte 5S RNA. A tryptic fragment of TFIIIA containing zinc fingers 4 through 7 remained tightly associated with somatic 5S RNA. Zinc fingers 4 through 7 may represent a tightly binding site for 5S RNA in the same sense that fingers 1 through 3 represent a tightly binding site for 5S DNA.
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Nogaroto, Viviane, Larissa Glugoski, Orlando Moreira Filho, and Marcelo Ricardo Vicari. "rDNA 5S e rearranjos cromossômicos em Rineloricaria (Siluriformes: Loricariidae)." Semina: Ciências Biológicas e da Saúde 38, no. 1supl (February 16, 2018): 230. http://dx.doi.org/10.5433/1679-0367.2017v38n1suplp230.
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Dados citogenéticos de Loricariidae (Siluriformes) revelam uma extensiva variação de número diploide e plasticidade cromossômica no grupo. Nestes peixes, a localização in situ de sítios de rDNA 5S em regiões co-localizadas a sítios teloméricos intersticiais (ITS) evidenciaram o envolvimento de múltiplos clusters de rDNA 5S em rearranjos cromossômicos nas subfamílias Loricariinae e Ancistrinae. Vestígios de sequências (TTAGGG)n em sítios internos podem corresponder à locais de rearranjos cromossômicos e também são considerados como pontos quentes para quebras cromossômicas, os “sítios frágeis”. Este trabalho teve como objetivo a caracterização molecular de segmentos duplicados (SD) de rDNA 5S, discutir seu envolvimento como sítio frágil, além da sua localização in situ em duas populações de Rineloricaria latirostris. Rineloricaria latirostris (rio das Pedras) apresentou 2n = 46 cromossomos e 5 pares marcados com rDNA 5S, além de um par portador de ITS/rDNA 5S co-localizado, enquanto R. latirostris (rio Piumhi) apresentou 2n = 48 cromossomos, 2 pares com sítios para rDNA 5S e ausência de ITS. Fragmentos isolados de rDNA 5S de 222 pb mostraram identidade com rDNAs 5S de outras espécies, além disso um amplificado de 702 pb apresentou inserção de um segmento do TE hAT em sua sequência, clone rDNA 5S degenerado. Ensaios de dupla-FISH evidenciaram a co-localização de rDNA 5S/rDNA 5S degenerado, rDNA 5S/hAT e ITS/rDNA 5S em R. latirostris do rio das Pedras, enquanto na outra população estudada apenas marcações de rDNA 5S foram detectadas. Segundo nossos dados, a invasão do TE hAT no rDNA 5S gerou a região de SD rDNA 5S degenerado, a qual atuou como sítio frágil para quebras cromossômicas e fusão Robertsoniana (Rb) em Rineloricaria. Assim, a evidência do SD rDNA 5S em pontos de fusão Rb em outras espécies sugerem que esta região é um sítio frágil para genomas de Loricariidae e, poderia explicar parte dos eventos Robertsonianos neste grupo de peixes.APOIO: Fundação Araucária, CNPq.
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Steitz, J. A., C. Berg, J. P. Hendrick, H. La Branche-Chabot, A. Metspalu, J. Rinke, and T. Yario. "A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells." Journal of Cell Biology 106, no. 3 (March 1, 1988): 545–56. http://dx.doi.org/10.1083/jcb.106.3.545.
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A novel 5S RNA-protein (RNP) complex in human and mouse cells has been analyzed using patient autoantibodies. The RNP is small (approximately 7S) and contains most of the nonribosome-associated 5S RNA molecules in HeLa cells. The 5S RNA in the particle is matured at its 3' end, consistent with the results of in vivo pulse-chase experiments which indicate that this RNP represents a later step in 5S biogenesis than a previously described 5S*/La protein complex. The protein moiety of the 5S RNP has been identified as ribosomal protein L5, which is known to be released from ribosomes in a complex with 5S after various treatments of the 60S subunit. Indirect immunofluorescence indicates that the L5/5S complex is concentrated in the nucleolus. L5 may therefore play a role in delivering 5S rRNA to the nucleolus for assembly into ribosomes.
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Wich, Günter, Lionel Sibold, and August Böck. "Divergent Evolution of 5 S rRNA Genes in Methanococcus." Zeitschrift für Naturforschung C 42, no. 4 (April 1, 1987): 373–80. http://dx.doi.org/10.1515/znc-1987-0408.
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Abstract The organization of genes for 5S rRNA in the methanogenic archaebacterium Methanococcus (M .) voltae and their nucleotide sequences have been determined. M. voltae possesses three 5S rRNA genes, one of them is organized in an rRNA transcriptional unit coding for 16S-23S-5S rRNA. The other two are associated with seven tRNA genes in a putative transcriptional unit composed of 5′-tRNAThr-tRNAPro-tRNATyr-tRNALys - 5S rRNA-tRNAAsp-tRNALys - 5S rRNA-tRNAAsp-3′. Coding regions plus spacers of the tRNALys-5S rRNA-tRNAAsp block of this gene cluster occur twice with identical sequence. The 5S rRNA from this cluster displays considerable sequence divergence to the rRNA operon-linked 5S rRNA gene. Comparison of the M. voltae 5S rRNA sequences with those from M. vannielii revealed that the operon-linked genes on one hand and the tRNA-linked 5S genes on the other share a greater sequence homology than the two types of genes within each of the two organisms. This indicates an independent evolution of the two sets of 5S rRNA genes without selective pressure from other ribosomal components or, alternatively, lateral gene transfer.
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Samson, M. L., and M. Wegnez. "Bipartite structure of the 5S ribosomal gene family in a Drosophila melanogaster strain, and its evolutionary implications." Genetics 118, no. 4 (April 1, 1988): 685–91. http://dx.doi.org/10.1093/genetics/118.4.685.
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Abstract Knowledge of multigenic family organization should provide insight into their mode of evolution. Accordingly, we characterized the 5S ribosomal gene family in the Drosophila melanogaster strain ry506. The 5S genes in this strain display a striking HindIII restriction difference compared to the "standard" D. melanogaster 5S genes. The sequence of three ry506 5S genes was determined. We show that the HindIII restriction site heterogeneity within the ry506 5S family most probably results from the same point mutation, suggesting that a single 5S variant was propagated into the 5S cluster of this strain. Furthermore, we demonstrate that the structural organization of the 5S genes in ry506 is a bipartite structure, i.e., that about 40% of the 5S genes constitute a HindIII+/HindIII- mixed cluster, while those remaining constitute an homogeneous HindIII- cluster. The events which might lead to such an heterogeneous pattern are discussed from an evolutionary point of view.
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Wasko, Adriane Pinto, Cesar Martins, Jonathan M. Wright, and Pedro Manoel Galetti Jr. "Molecular organization of 5S rDNA in fishes of the genus Brycon." Genome 44, no. 5 (October 1, 2001): 893–902. http://dx.doi.org/10.1139/g01-067.
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There are few reports on the genomic organization of 5S rDNA in fish species. To characterize the 5S rDNA nucleotide sequence and chromosomal localization in the Neotropical fishes of the genus Brycon, 5S rDNA copies from seven species were generated by PCR. The nucleotide sequences of the coding region (5S rRNA gene) and the nontranscribed spacer (NTS) were determined, revealing that the 5S rRNA genes were highly conserved, while the NTSs were widely variable among the species analyzed. Moreover, two classes of NTS were detected in each species, characterized by base substitutions and insertionsdeletions. Using fluorescence in situ hybridization (FISH), two 5S rDNA chromosome loci that could be related to the two 5S rDNA NTS classes were observed in at least one of the species studied. 5S rDNA sequencing and chromosomal localization permitted the characterization of Brycon spp. and suggest a higher similarity among some of them. The data obtained indicate that the 5S rDNA can be an useful genetic marker for species identification and evolutionary studies.Key words: Brycon, FISH, nontranscribed spacer, nucleotide sequence, 5S rDNA.
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Choi, Young A., Ryutaro Tao, Keizo Yonemori, and Akira Sugiura. "Simultaneous Visualization of 5S and 45S rDNAs in Persimmon (Diospyros kaki) and Several Wild Relatives (Diospyros spp.) by Fluorescent in situ Hybridization (FISH) and MultiColor FISH (MCFISH)." Journal of the American Society for Horticultural Science 128, no. 5 (September 2003): 736–40. http://dx.doi.org/10.21273/jashs.128.5.0736.
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5S ribosomal DNA (rDNA) was visualized on the somatic metaphase chromosome of persimmon (Diospyros kaki) and ten wild Diospyros species by fluorescent in situ hybridization (FISH). The digoxigenin (DIG)-labeled 5S rDNA probe was hybridized onto the chromosomes and visualized by incubation with anti-DIG-fluorescein isothiocyanate (FITC). Strong signals of 5S rDNA probe were observed on several chromosomes of Diospyros species tested. Furthermore, multicolor FISH using 5S and 45S rDNA probes differently labeled with DIG and biotin, revealed separate localization of the two rDNA genes on different chromosomes of Diospyros species tested, suggesting that 5S and 45S rDNA sites can be used as chromosome markers in Diospyros. The number of 5S rDNA sites varied with the Diospyros species. More 5S rDNA sites were observed in four diploid species native to Southern Africa than in three Asian diploid species. The former had four or six 5S rDNA sites while the latter had two. Three Asian polyploidy species had four to eight 5S rDNA sites. Among the Asian species, the number of 5S rDNA sites seemed to increase according to ploidy level of species. These features of 5S rDNA sites were very similar to those of 45S rDNA sites in Diospyros. Phylogenetic relationship between D. kaki and wild species tested are discussed based on the number and chromosomal distribution of 5S and 45S rDNA.
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Peck, L. J., L. Millstein, P. Eversole-Cire, J. M. Gottesfeld, and A. Varshavsky. "Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro." Molecular and Cellular Biology 7, no. 10 (October 1987): 3503–10. http://dx.doi.org/10.1128/mcb.7.10.3503-3510.1987.
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An extract from whole oocytes of Xenopus laevis was shown to transcribe somatic-type 5S RNA genes approximately 100-fold more efficiently than oocyte-type 5S RNA genes. This preference was at least 10-fold greater than the preference seen upon microinjection of 5S RNA genes into oocyte nuclei or upon in vitro transcription in an oocyte nuclear extract. The approximately 100-fold transcriptional bias in favor of the somatic-type 5S RNA genes observed in vitro in the whole oocyte extract was similar to the transcriptional bias observed in developing Xenopus embryos. We also showed that in the whole oocyte extract, a promoter-binding protein required for 5S RNA gene transcription, TFIIIA, was bound both to the actively transcribed somatic-type 5S RNA gene and to the largely inactive oocyte-type 5S RNA genes. These findings suggest that the mechanism for the differential expression of 5S RNA genes during Xenopus development does not involve differential binding of TFIIIA to 5S RNA genes.
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Fukui, K., Y. Kamisugi, and F. Sakai. "Physical mapping of 5S rDNA loci by direct-cloned biotinylated probes in barley chromosomes." Genome 37, no. 1 (February 1, 1994): 105–11. http://dx.doi.org/10.1139/g94-013.
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5S rDNA loci have been mapped on barley chromosomes by in situ hybridization using five reciprocal translocation lines. Two kinds of DNA probes covering either the 5S rDNA coding region or the 5S rDNA coding and flanking noncoding regions were used. They were prepared by direct cloning from interphase nuclei and simultaneous direct labeling in PCR. Four 5S rDNA loci were detected in a haploid genome by the 5S rDNA coding region, whereas in addition, the four or six 5S rDNA related sites, depending on the variety used, were revealed by the probe covering the flanking region. The four 5S rDNA loci revealed and mapped on the barley chromosomes: 2 (2I), 3 (3I), 1 (7I), and 4 (4I) were designated 5SRrn-I1, 5SRrn-I2, 5SRrn-I3 and 5SRrn-I4, respectively, in descending order of copy number of 5S rRNA genes.Key words: Hordeum vulgare, 5S rDNA, in situ hybridization, direct cloning, direct labeling.
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Peck, L. J., L. Millstein, P. Eversole-Cire, J. M. Gottesfeld, and A. Varshavsky. "Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro." Molecular and Cellular Biology 7, no. 10 (October 1987): 3503–10. http://dx.doi.org/10.1128/mcb.7.10.3503.
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An extract from whole oocytes of Xenopus laevis was shown to transcribe somatic-type 5S RNA genes approximately 100-fold more efficiently than oocyte-type 5S RNA genes. This preference was at least 10-fold greater than the preference seen upon microinjection of 5S RNA genes into oocyte nuclei or upon in vitro transcription in an oocyte nuclear extract. The approximately 100-fold transcriptional bias in favor of the somatic-type 5S RNA genes observed in vitro in the whole oocyte extract was similar to the transcriptional bias observed in developing Xenopus embryos. We also showed that in the whole oocyte extract, a promoter-binding protein required for 5S RNA gene transcription, TFIIIA, was bound both to the actively transcribed somatic-type 5S RNA gene and to the largely inactive oocyte-type 5S RNA genes. These findings suggest that the mechanism for the differential expression of 5S RNA genes during Xenopus development does not involve differential binding of TFIIIA to 5S RNA genes.
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Khoryanton, Ampala, Sri Harmanto, and Ignatius W. Gunawan. "Assessment Standards for 5S Implementation on SMEs of Ship Component." Journal of Southwest Jiaotong University 56, no. 2 (April 30, 2021): 32–41. http://dx.doi.org/10.35741/issn.0258-2724.56.2.4.
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This article describes a new idea in applying the 5S concept (Seiri, Seiton, Seiso, Seiketsu, Shitsuke) to create a quality work environment for small and medium-sized enterprises of ship components. The success rate of implementing the 5S concept must be known based on assessing the effectiveness of the 5S concept application. Assessment of the effectiveness of the application of the 5S concept requires measurement indicators that are with the characteristics work environment of small and medium-sized enterprises of ship components. Determination of standard indicators for the implementation of 5S enabling to assessing the effectiveness of the implementation of 5S repeatedly and consistently. Indicators for the assessment of 5S effectiveness are obtained using the Delphi method. The results showed that the Delphi method obtained 37 indicators that can be used to assess the effectiveness of the implementation of 5S on small and medium-sized enterprises of ship components with an agreement of 75% to 100%. This assessment standard can be used in every phase of the 5S implementation, namely the initial implementation stage up to the system maturity stage. This assessment standard can also be used in similar small and medium-sized enterprises implementing 5S.
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Christian, Rio Surya. "PENERAPAN EVALUASI RINGKAS, RAPI, RESIK, RAWAT, RAJIN PT. INKA (PERSERO) MADIUN." Indonesian Journal of Occupational Safety and Health 7, no. 1 (October 31, 2018): 11. http://dx.doi.org/10.20473/ijosh.v7i1.2018.11-19.
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Sort, Set, Shine, Standardize, and Sustain (5S) program is an attempt to minimize or eliminate harm by creating a good workplace culture in the workplace. A comfortable working environment will improve efficiency, productivity, quality, and safety. The 5S program is one of the administrative controls used to prevent accidents. 5S implementation will be reached and applied with support from all sides involved, start from top management, middle management, and low management. The study aims to evaluate the application of 5S in PT. INKA (Persero) Madiun. This study was observational and descriptive research using cross sectional design. The results showed that the application of 5S at PT. INKA (Persero)Madiun had been done since the first time but there wasn’t 5S activity. In 2014, the company's policy on 5R implementation appear so that the 5S implementation was fully supported by the company. Evaluation of the 5S implementation involves 5 areas include open area and workshop area, that already apply 5S well. The conclusions of this study was implementation 5S at PT. INKA (Persero) Madiun need to increase and support from top level management. Keywords: set, shine, sort, standardize, sustain
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Drouin, Guy. "Expressed retrotransposed 5S rRNA genes in the mouse and rat genomes." Genome 43, no. 1 (February 1, 2000): 213–15. http://dx.doi.org/10.1139/g99-100.
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The analyses of previously described 5S rRNA gene sequences show that some of the expressed 5S rRNA genes present in the mouse and rat genomes were derived from the retrotransposition of 5S rRNA transcripts. These analyses demonstrate that new 5S rRNA gene copies can originate by retrotransposition and that some of these retrotranscribed genes are expressed. Key words: 5S ribosomal RNA genes, retrotransposition, retroposons.
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Gunawan, Muhkhammat Sahrul. "ANALISIS PENERAPAN 5R PADA PT SUKUN TRANSPORT LOGISTIK." Journal of Industrial Engineering and Technology 1, no. 2 (October 4, 2021): 27–37. http://dx.doi.org/10.24176/jointtech.v1i2.6495.
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5S yang dikembangkan di jepang, disebut 5S (Seiri, Seiton, Seiso, Seiketsu, dan Shitsuke) yang merupakan sarana mencapai Efisiensi, Produktivitas, Kualitas, dan Keselamatan Kerja. Pengambilan topik 5S pada laporan ini dilakukan berdasar penghayatan bahwa tenaga kerja plus budaya 5S merupakan harta utama bagi perusahaan. Tujuan dari laporan ini adalah untuk mengetahui kondisi area kerja di perusahaan PT Sukun Transport logistik dengan penerapan 5S. Kelebihan dari adanya implementasi tersebut antara lain membantu perusahaan dalam membenahi pola pikir pekerja terhadap pembiasaan 5S di area kerja. Sedangkan untuk kekurangan dari implementasinya adalah semua pihak harus melibatkan dirinya dalam penerapan 5S dengan cara meluangkan sebagian waktunya dalam menunjang kerapian dan kebersihan di area kerja. Penerapan 5S yang dilakukan oleh perusahaan adalah dengan melakukan pembuatan alur pelaksanaan 5S dan kebersihan terhadap lantai yang yang kurang bersih di area bengkel. Selain itu, perusahaan sudah melakukan pembuatan layout untuk area bengkel. Adapun rekomendasi perbaikan yang digunakan sebagai penunjang pelaksanaannya antara lain menyusun standarisasi dan prosedur pelaksanaan, melaksanakan training, menerapkan layout tetap, penetapan jadwal inspeksi, dan penentukan jadwal piket.
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MORENO-CAMPOS, RODRIGO, LUIS E. FLORENCIO-MARTÍNEZ, TOMÁS NEPOMUCENO-MEJÍA, SAÚL ROJAS-SÁNCHEZ, DANIEL E. VÉLEZ-RAMÍREZ, NORMA E. PADILLA-MEJÍA, ELISA FIGUEROA-ANGULO, REBECA MANNING-CELA, and SANTIAGO MARTÍNEZ-CALVILLO. "Molecular characterization of 5S ribosomal RNA genes and transcripts in the protozoan parasite Leishmania major." Parasitology 143, no. 14 (October 6, 2016): 1917–29. http://dx.doi.org/10.1017/s0031182016001712.
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SUMMARYEukaryotic 5S rRNA, synthesized by RNA polymerase III (Pol III), is an essential component of the large ribosomal subunit. Most organisms contain hundreds of 5S rRNA genes organized into tandem arrays. However, the genome of the protozoan parasite Leishmania major contains only 11 copies of the 5S rRNA gene, which are interspersed and associated with other Pol III-transcribed genes. Here we report that, in general, the number and order of the 5S rRNA genes is conserved between different species of Leishmania. While in most organisms 5S rRNA genes are normally associated with the nucleolus, combined fluorescent in situ hybridization and indirect immunofluorescence experiments showed that 5S rRNA genes are mainly located at the nuclear periphery in L. major. Similarly, the tandemly repeated 5S rRNA genes in Trypanosoma cruzi are dispersed throughout the nucleus. In contrast, 5S rRNA transcripts in L. major were localized within the nucleolus, and scattered throughout the cytoplasm, where mature ribosomes are located. Unlike other rRNA species, stable antisense RNA complementary to 5S rRNA is not detected in L. major.
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Gottlob-McHugh, S. G., M. Lévesque, K. MacKenzie, M. Olson, O. Yarosh, and D. A. Johnson. "Organization of the 5S rRNA genes in the soybean Glycine max (L.) Merrill and conservation of the 5S rDNA repeat structure in higher plants." Genome 33, no. 4 (August 1, 1990): 486–94. http://dx.doi.org/10.1139/g90-072.
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The 5S rRNA gene of the soybean Glycine max (L.) Merr. has been cloned on a 556-bp fragment of DNA and sequenced. This fragment contains two copies of the soybean 5S rDNA sequence, one intact and one truncated, separated by noncoding DNA. We have used this clone to investigate the organization of the 5S genes within the soybean genome and the extent of their methylation. Our results demonstrate that soybean 5S genes are clustered, organized into tandem repeats of 330 bp, and extensively methylated. Hybridization of the 5S sequence to Southern transfers of soybean DNA digested with BamHI reveals a striking ladderlike pattern. Hybridization of the soybean 5S sequence to a wide variety of plant DNAs results in similar patterns, suggesting that the 5S rDNA sequence, gene organization, and methylation pattern are conserved in many higher plants.Key words: 5S rDNA, sequence, methylation, soybean, repeat conservation.
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Stratiichuk, A. S., T. O. Derevenko, and Y. O. Tynkevych. "Organization of 5S rDNA repeated unit of Quercus imbricaria Michx." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 17, no. 2 (January 20, 2020): 179–86. http://dx.doi.org/10.7124/visnyk.utgis.17.2.1219.
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Aim. The 5S rDNA repeats represent a universal model for the investigation of molecular evolution of repeated sequences. Also, comparison of 5S rDNA was successfully applied for the elucidation of phylogenetic relationships between the closely related plant species. However, there is practically no data regarding the molecular organization of 5S rDNA repeats in members of the section Lobatae, one of the largest groups of the genus Quercus. Accordingly, our aim was to investigate the 5S rDNA organization for Q. imbricaria, a species that belongs to this section. Methods. DNA extraction, PCR amplification, cloning and sequencing. Results. A complete 5S rDNA repeat of Q. imbricaria was cloned and sequenced. It has been found that in the oak genome, the 5S rDNA coding region contains five nucleotide substitutions as compared to that in Arabidopsis. Nevertheless, the predicted secondary structure of the transcript retains all typical features of 5S rRNA. Presumptive sequence elements of the external promoter were identified in the IGS. Conclusions. The nucleotide substitutions that occur in the 5S rRNA during evolution appear to be compensatory, resulting in conservation of its secondary structure. Due to considerable differences among the species of different sections, the 5S rDNA IGS can be applied for the taxonomic studies in the genus Quercus. Keywords: 5S rDNA, molecular evolution, Quercus, Lobatae.
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Gupta, Shaman, and Sanjiv Kumar Jain. "An application of 5S concept to organize the workplace at a scientific instruments manufacturing company." International Journal of Lean Six Sigma 6, no. 1 (March 2, 2015): 73–88. http://dx.doi.org/10.1108/ijlss-08-2013-0047.
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Purpose – The purpose of this paper is to use the 5S tool to assist a small-scale manufacturing organization to become more productive and more efficient. Design/methodology/approach – A simple approach has been adopted to create the teams for implementing 5S. Cause-and-effect diagram has been studied for shop floor analysis. Later, four data collection methods have been used to ensure right implementation of the 5S. Findings – In the frames of this case study, it has been analyzed that implementation of “5S” resulted in overall improvement of the organization. With the implementation of “5S”, major benefits in the form of tool searching time have been achieved. Tool searching time from shop floor has been reduced from 30 minutes to 5 minutes. “5S” audit has been conducted in the organization. “5S” audit score has been increased from 7 (Week 1) to 55 (Week 20). Practical implications – 5S is a powerful tool and can be implemented in various industries whether micro, small, medium or large. Implementation of 5S has large horizontal development and can be implemented in all the workstations of an organization. Originality/value – The publications and case study presented in this paper will be useful to researchers, professionals and others concerned with this subject to understand the significance of 5S.
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Ishchenko, O. O., V. V. Kozub, and I. I. Panchuk. "Organization of 5S ribosomal DNA of Litchi chinensis Sonn." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 18, no. 1-2 (January 29, 2021): 3–8. http://dx.doi.org/10.7124/visnyk.utgis.18.1-2.1348.
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Aim. 5S ribosomal DNA (5S rDNA) represents a universal model for studying the evolution of repeated sequences in eukaryotic organisms. Taking into account that this region of the genome still remains almost undescribed in species of the family Sapindaceae, we investigated the molecular organization of a repeated unit of 5S rDNA in a member of this family, Litchi chinensis. Methods. PCR amplification, cloning and sequencing of 5S rDNA. Results. It was found that the length of the repeated unit of the 5S rDNA of L. chinensis is 321 -323 bp. The level of intragenomic similarity of 5S rDNA repeats is 87.1 %. Potential external elements of the RNA polymerase III promoter, which are localized in IGS, differ from those described for members of other families of angiosperms. Conclusions. In the genome of L. chinensis, at least two classes of 5S rDNA repeats are present, which differ in the sequence of external promoter elements.
Keywords: 5S rDNA, molecular evolution, Litchi chinensis, Sapindaceae.
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Darby, M. K., and K. E. Joho. "Differential binding of zinc fingers from Xenopus TFIIIA and p43 to 5S RNA and the 5S RNA gene." Molecular and Cellular Biology 12, no. 7 (July 1992): 3155–64. http://dx.doi.org/10.1128/mcb.12.7.3155-3164.1992.
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Zinc fingers are usually associated with proteins that interact with DNA. Yet in two oocyte-specific Xenopus proteins, TFIIA and p43, zinc fingers are used to bind 5S RNA. One of these, TFIIIA, also binds the 5S RNA gene. Both proteins have nine zinc fingers that are nearly identical with respect to size and spacing. We have determined the relative affinities of groups of zinc fingers from TFIIIA for both 5S RNA and the 5S RNA gene. We have also determined the relative affinities of groups of zinc fingers from p43 for 5S RNA. The primary protein regions for RNA and DNA interaction in TFIIIA are located at opposite ends of the molecule. All zinc fingers from TFIIIA participate in binding 5S RNA, but zinc fingers from the C terminus have the highest affinity. N-terminal zinc fingers are essential for binding the 5S RNA gene. In contrast, zinc fingers at the amino terminus of p43 are essential for binding 5S RNA.
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Darby, M. K., and K. E. Joho. "Differential binding of zinc fingers from Xenopus TFIIIA and p43 to 5S RNA and the 5S RNA gene." Molecular and Cellular Biology 12, no. 7 (July 1992): 3155–64. http://dx.doi.org/10.1128/mcb.12.7.3155.
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Zinc fingers are usually associated with proteins that interact with DNA. Yet in two oocyte-specific Xenopus proteins, TFIIA and p43, zinc fingers are used to bind 5S RNA. One of these, TFIIIA, also binds the 5S RNA gene. Both proteins have nine zinc fingers that are nearly identical with respect to size and spacing. We have determined the relative affinities of groups of zinc fingers from TFIIIA for both 5S RNA and the 5S RNA gene. We have also determined the relative affinities of groups of zinc fingers from p43 for 5S RNA. The primary protein regions for RNA and DNA interaction in TFIIIA are located at opposite ends of the molecule. All zinc fingers from TFIIIA participate in binding 5S RNA, but zinc fingers from the C terminus have the highest affinity. N-terminal zinc fingers are essential for binding the 5S RNA gene. In contrast, zinc fingers at the amino terminus of p43 are essential for binding 5S RNA.
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Bogdanov, Alexey A., Olga A. Dontsova, Svetlana S. Dokudovskaya, and Inna N. Lavrik. "Structure and function of 5S rRNA in the ribosome." Biochemistry and Cell Biology 73, no. 11-12 (December 1, 1995): 869–76. http://dx.doi.org/10.1139/o95-094.
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5S rRNA is a small RNA molecule that is a component of a ribosome from almost all living organisms. In this review, we discuss the biogenesis of 5S rRNA and its properties as an independent structural domain of a ribosome as well as the current concepts concerning the higher order structure of 5S rRNA in free state and in its complexes with ribosomal proteins and its folding in the ribosome. Special attention is paid to recent experimental approaches that have been useful in 5S rRNA studies. Our own data on topography of 5S rRNA in the ribosomes are discussed in detail. The hypothesis describing the possible functional role of 5S rRNA for ribosome functioning is discussed.Key words: 5S rRNA, ribosomes, 23S rRNA, site-directed chemical cross-linking, RNA folding.
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Specht, T., J. Wolters, and V. A. Erdmann. "Compilation of 5S rRNA and 5S rRNA gene sequences." Nucleic Acids Research 18, suppl (April 25, 1990): 2215–30. http://dx.doi.org/10.1093/nar/18.suppl.2215.
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Specht, T., J. Wolters, and V. A. Erdmann. "Compilation of 5S rRNA and 5S rRNA gene sequences." Nucleic Acids Research 19, suppl (April 25, 1991): 2189–91. http://dx.doi.org/10.1093/nar/19.suppl.2189.
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Wolters, J., and V. A. Erdmann. "Compilation of 5S rRNA and 5S rRNA gene sequences." Nucleic Acids Research 16, suppl (January 1, 1988): r1—r70. http://dx.doi.org/10.1093/nar/16.suppl.r1.
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Specht, T., M. Szymanski, M. Z. Barciszewska, J. Barciszewski, and V. A. Erdmann. "Compilation of 5S rRNA and 5S rRNA gene sequences." Nucleic Acids Research 25, no. 1 (January 1, 1997): 96–97. http://dx.doi.org/10.1093/nar/25.1.96.
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Wang, Lei, Martin Ciganda, and Noreen Williams. "Defining the RNA-Protein Interactions in the Trypanosome Preribosomal Complex." Eukaryotic Cell 12, no. 4 (February 8, 2013): 559–66. http://dx.doi.org/10.1128/ec.00004-13.
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ABSTRACT In eukaryotes, 5S rRNA is transcribed in the nucleoplasm and requires the ribosomal protein L5 to deliver it to the nucleolus for ribosomal assembly. The trypanosome-specific proteins P34 and P37 form a novel preribosomal complex with the eukaryotic conserved L5-5S rRNA complex in the nucleoplasm. Previous results suggested that P34 acts together with L5 to bridge the interaction with 5S rRNA and thus to stabilize 5S rRNA, an important role in the early steps of ribosomal biogenesis. Here, we have delineated the domains of the two protein components, L5 and P34, and regions of the RNA partner, 5S rRNA, that are critical for protein-RNA interactions within the complex. We found that the L18 domain of L5 and the N terminus and RNA recognition motif of P34 bind 5S rRNA. We showed that Trypanosoma brucei L5 binds the β arm of 5S rRNA, while P34 binds loop A/stem V of 5S rRNA. We demonstrated that 5S rRNA is able to enhance the association between the protein components of the complex, L5 and P34. Both loop A/stem V and the β arm of 5S rRNA can separately enhance the protein-protein association, but their effects are neither additive nor synergistic. Domains in the two proteins for protein-protein and protein-RNA interactions overlap or are close to each other. This suggests that 5S rRNA binding might cause conformational changes in L5 and P34 and might also bridge the interactions, thus enhancing binding between the protein partners of this novel complex.
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Qowim, Miftahul, Nina Aini Mahbubah, and M. Zainuddin Fathoni. "PENERAPAN 5S PADA DIVISI GUDANG (STUDI KASUS PT. SUMBER URIP SEJATI)." JUSTI (Jurnal Sistem dan Teknik Industri) 1, no. 1 (November 13, 2020): 49. http://dx.doi.org/10.30587/justicb.v1i1.2032.
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Dalam meningkatkan mutu, selalu ada banyak cara dan alat yang dapat digunakan, mutu untuk lingkungan tempat kerja 5S (Seiri, Seiton, Seiso, Seketsu, Shitsuke) merupakan salah salah satu metode yang telah efektif dalam manajemen penataan gudang. PT. sumber urip sejati telah mengimplementasikan pendekatan 5S pada seluruh devisi. Namun pada devisi gudang tiga tidak dilakukan evaluasi dan assessment 5S. untuk mengetahui hasil implementasi 5S, maka dilakukan evaluasi dan assesment 5S (Seiri, Seiton, Seiso, Seketsu, Shitsuke) pada divisi gudang tiga. selain hal tersebut peneliti juga memberikan usulan perbaikan penerapan 5S. Penelitian menggunakan pendekatan deskriptif kualitatif untuk mengevaluasi implementasi 5S.Hasil evaluasi dari penelitian diperoleh kategori evaluasi implementasi 5S adalah Seiri dalam kategori cukup dengan skor 59%, Seiton dalam kategori cukup dengan skor 49%, Seiso dalam kategori cukup dengan skor 48%, Seiketsu dalam kategori cukup dengan skor 50%, Shitsuke dalam kategori cukup dengan skor 44%.
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Nguyễn, Quỳnh Trúc. "KIẾN THỨC VỀ 5S VÀ MỘT SỐ YẾU TỐ LIÊN QUAN ĐẾN KIẾN THỨC 5S CỦA NHÂN VIÊN Y TẾ TẠI MỘT SỐ BỆNH VIỆN TUYẾN HUYỆN, THÀNH PHỐ CẦN THƠ, NĂM 2022." Tạp chí Y Dược học Cần Thơ, no. 50 (August 20, 2022): 150–56. http://dx.doi.org/10.58490/ctump.2022i50.137.
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Đặt vấn đề: Sàng lọc, Sắp xếp, Sạch sẽ, Săn sóc, Sẵn sàng (5S) là nền tảng của phương pháp chăm sóc sức khỏe tinh gọn. Hiện nay, 5S được áp dụng cho lĩnh vực chăm sóc sức khỏe như một phương pháp tổ chức và chuẩn hóa tại Bệnh viện. Mục tiêu nghiên cứu: Xác định tỷ lệ đáp ứng 5S và phân tích một số yếu tố liên quan đến kiến thức 5S của nhân viên y tế (NVYT) tại Bệnh viện tuyến huyện thuộc thành phố Cần Thơ. Đối tượng và phương pháp nghiên cứu: Nghiên cứu mô tả cắt ngang trên 164 NVYT hiện đang làm việc tại hai bệnh viện tuyến huyện, thành phố Cần Thơ năm năm 2020. Các khảo sát được thực hiện trực tuyến qua Google Form; các tiêu chí khảo sát và đánh giá sử dụng bảng kiểm 5S của Cơ quan Hợp tác Quốc tế Nhật Bản. Kết quả: Tỷ lệ NVYT có kiến thức đúng về 5S đạt 55,5%, Các yếu tố Sạch sẽ, Sàng lọc đạt tỷ lệ tương ứng 66,46% và 48,78%. Các yếu tố liên quan đến tỷ lệ kiến thức 5S đạt là giới tính, nhóm tuổi và lĩnh vực công tác. Nam giới có kiến thức 5S đạt bằng 0,43 lần so với nữ giới. Kiến thức 5S ở nhóm tuổi 40-49 tuổi gấp 7,22 lần nhóm dưới 30 tuổi. NVYT công tác khám chữa bệnh có kiến thức 5S đạt 1,96 lần so với nhân viên hành chính. Kết luận: Cần tăng cường nâng cao kiến thức 5S cho NVYT. Các giải pháp cải tiến 5S cần tập trung vào nhóm nhân viên hành chính, độ tuổi dưới 30 và nhóm tiêu chí Sàng lọc.
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Lysenko, Oleksandr, Dmytro Lebedev, Volodymyr Davydenko, and Anatoliy Miroshnychenko. "Secrets of successful implementation of 5S." Electronic Scientific Journal Intellectualization of Logistics and Supply Chain Management #1 2020, no. 14 (October 2022): 31–39. http://dx.doi.org/10.46783/smart-scm/2022-14-3.
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The article is devoted to the analysis of the possibilities of implementing 5S at the organization. The component of the 5S system is considered. The article describes the theoretical and practical aspects of the transition to the 5S system. Classical approaches are highlighted, the main reasons that lead to the occurrence of resource losses are considered. An analysis of the basic tools and methods that can be applied during the implementation of the transition to the 5S system was carried out. The possibilities of obtaining benefits from the introduction of the 5S system are considered. Approaches to using the system of organization and rationalization of the 5S workplace in practice were proposed. Recommendations for further research are provided.
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Martins, Cesar, and Pedro Manoel Galetti Jr. "Organization of 5S rDNA in species of the fish Leporinus: two different genomic locations are characterized by distinct nontranscribed spacers." Genome 44, no. 5 (October 1, 2001): 903–10. http://dx.doi.org/10.1139/g01-069.
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To address understanding the organization of the 5S rRNA multigene family in the fish genome, the nucleotide sequence and organization array of 5S rDNA were investigated in the genus Leporinus, a representative freshwater fish group of South American fauna. PCR, subgenomic library screening, genomic blotting, fluorescence in situ hybridization, and DNA sequencing were employed in this study. Two arrays of 5S rDNA were identified for all species investigated, one consisting of monomeric repeat units of around 200 bp and another one with monomers of 900 bp. These 5S rDNA arrays were characterized by distinct NTS sequences (designated NTS-I and NTS-II for the 200- and 900-bp monomers, respectively); however, their coding sequences were nearly identical. The 5S rRNA genes were clustered in two chromosome loci, a major one corresponding to the NTS-I sites and a minor one corresponding to the NTS-II sites. The NTS-I sequence was variable among Leporinus spp., whereas the NTS-II was conserved among them and even in the related genus Schizodon. The distinct 5S rDNA arrays might characterize two 5S rRNA gene subfamilies that have been evolving independently in the genome.Key words: 5S rDNA, 5S rRNA gene, nontranscribed spacer, Leporinus, fish.
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Faradilla, Arnes, Winnie Septiani, Nora Azmi, and Syafa Kansa. "PERANCANGAN RUANG KERJA DOSEN DAN PENATAAN DOKUMEN MENGGUNAKAN METODE 5S DI JURUSAN TEKNIK INDUSTRI, UNIVERSITAS TRISAKTI." J@ti Undip : Jurnal Teknik Industri 14, no. 2 (May 31, 2019): 81. http://dx.doi.org/10.14710/jati.14.2.81-86.
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Setiap Dosen memiliki tugas utama terkait dengan tridharma yaitu Pendidikan dan Pengajaran, Penelitian, Pengabdian Kepada Masyarakat (PKM), dan Penunjang Tridharma. Dokumen yang digunakan banyak dan harus disimpan dengan rapi di ruang kerja untuk keperluan kegiatan Dosen seperti Beban Kerja Dosen (BKD) dan kepangkatan. Hasil penelitian pendahuluan menunjukkan sebanyak 62,5% Dosen menyatakan kesulitan dalam menyimpan dokumen di ruang kerja dan sebanyak 81,25% menyatakan perlu adanya perbaikan di ruang kerja. Penelitian ini bertujuan untuk merancang ruang kerja Dosen dan penataan dokumen menggunakan metode 5S. Penelitian diawali dengan penguraian aktivitas Dosen dan pengelompokkan dokumen, perancangan ruang kerja dan fasilitas penyimpanan dokumen, identifikasi kondisi awal ruang kerja, penerapan 5S dan evaluasi hasil rancangan menggunakan Office 5S Audit Checklist. Penelitian ini menghasilkan penataan dokumen yang tersusun rapi berdasarkan prinsip 5S, rancangan almari dengan kriteria biaya yang tidak terlalu mahal, ukuran dan desain disesuaikan dengan ukuran ruangan dan dokumen yang akan disimpan. Hasil penilaian performansi 5S di ruang kerja Dosen sebelum penerapan 5S diperoleh skor rata-rata sebesar 56,83 dengan kategori rating fair, dan setelah penerapan 5S skor rata-rata menjadi 92 dengan kategori rating excellent yang menunjukan peningkatan sebesar 50%. Abstract[Planning Lecturer Working Room and Document Arrangement Using 5S Method In Department Industrial Engineering, Trisakti University] Every lecturer has main duty to fulfill Tridharma Perguruan Tinggi which are Education and Teaching, Research and PKM. All of the documents must be stored in order to easier the lecturer to necessity of BKD and raising the grade. The preliminary research about the lecture’s working room, the result stated that as much as 62.5% lectures have difficulties to store the documents in their working room. Several lecturers stated that the facilities such as board is unappropriate. Another 81.25% of lecturers stated is necessary to improve the system to store the documents their working room.The purpose of this research is to design the lecture’s working room and organize the documents using 5S method. The research is began with describing of lecturer activities and documents grouping, designing of lecturer’s room and facility of documents storage, initial identification of lecturer’s room, application of 5S method and evaluation of this application. This reseach has result of document structuring based on 5S method, document board which have criterion such as cheaper price, size and design appropriate with the room and documents will be stored. The result of 5S performance in lecturer’s room before using 5S method id 56.83 which is fair category, and after using 5S the score is increase become 92 which is excellent catogory. It can be conclude that there is increasing the score almost 50%.Keywords: ergonomic; office ergonomic; office 5S audit checklist; score 5S
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Oktafiani, Ilmiah Sholikhah, and Subiyantoro Subiyantoro. "Application of 5S Management in Improving the Quality of Furniture Production in Meubel Karya Agung of Ponorogo Regency." PALAPA 10, no. 1 (May 21, 2022): 45–60. http://dx.doi.org/10.36088/palapa.v10i1.1658.
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5S (Sort, Set in order, Shine, Standardize, dan Self Discipline) is a work culture that must be applied in all workplaces both in the formal and informal sectors. The implementation of 5S aims to make workers feel comfortable and safe while working. In addition, the application of 5S can make work more effective and efficient because it can eliminate wasted time due to more time to search for goods, work areas that look full due to excess equipment and un needed goods or dirty environments. Furniture Works In Semanding District, Kauman, Ponorogo is one of the informal sectors that have not implemented 5R. This is seen from the condition of the work area full of unnecessary equipment and goods, messy, and dirty. Therefore, researchers conducted research analysis of the application of 5S in Furniture Works. Purpose: The purpose of the research to know the application of the principle of 5S in Furniture Works. Method: This research uses qualitative design. The research was conducted with direct observation and using the 5S checklist. Research Results: The results showed that the application of 5S in Furniture Works is still not good. This is evident from the achievement of the 5S as a whole which is below 50%. Conclusion: The results of the study concluded that the implementation of 5S in Furniture Works is still lacking so it is necessary to conduct training on 5S and internal audits periodically.
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Guinta, D. R., and L. J. Korn. "Differential order of replication of Xenopus laevis 5S RNA genes." Molecular and Cellular Biology 6, no. 7 (July 1986): 2536–42. http://dx.doi.org/10.1128/mcb.6.7.2536-2542.1986.
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In Xenopus laevis there are two multigene families of 5S RNA genes: the oocyte-type 5S RNA genes which are expressed only in oocytes and the somatic-type 5S RNA genes which are expressed throughout development. The Xenopus 5S RNA replication-expression model of Gottesfeld and Bloomer (Cell 28:781-791, 1982) and Wormington et al. (Cold Spring Harbor Symp. Quant. Biol. 47:879-884, 1983) predicts that the somatic-type 5S RNA genes replicate earlier in the cell cycle than do the oocyte-type genes. Hence, the somatic-type 5S RNA genes have a competitive advantage in binding the transcription factor TFIIIA in somatic cells and are thereby expressed to the exclusion of the oocyte-type genes. To test the replication-expression model, we determined the order of replication of the oocyte- and somatic-type 5S RNA genes. Xenopus cells were labeled with bromodeoxyuridine, stained for DNA content, and then sorted into fractions of S phase by using a fluorescence-activated cell sorter. The newly replicated DNA containing bromodeoxyuridine was separated from the lighter, unreplicated DNA by equilibrium centrifugation and was hybridized with DNA probes specific for the oocyte- and somatic-type 5S RNA genes. In this way we found that the somatic-type 5S RNA genes replicate early in S phase, whereas the oocyte-type 5S RNA genes replicate late in S phase, demonstrating a key aspect of the replication-expression model.
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Srinivasan, Siddarth, Laura Hughes Ikuma, Mahmoud Shakouri, Isabelina Nahmens, and Craig Harvey. "5S impact on safety climate of manufacturing workers." Journal of Manufacturing Technology Management 27, no. 3 (April 4, 2016): 364–78. http://dx.doi.org/10.1108/jmtm-07-2015-0053.
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Purpose – 5S is a commonly used Lean tool that focusses on creating an organized work environment, but the effects of 5S on safety climate are not as well studied. The purpose of this paper is to determine the impact of a 5S event on safety climate. Design/methodology/approach – This pre-test post-test study examines the effect of implementing 5S on safety climate of the packaging area of a manufacturing plant. Two groups of employees (case and control groups) completed a safety climate questionnaire (Safety Climate Assessment Toolkit) prior to the 5S event, one month after, and two months after. Findings – Total safety climate significantly improved for the case group but remained unchanged for the control group over the study period. Specifically, management commitment and involvement dimensions of safety climate improved for the case group. Practical implications – These results show that two important aspects of safety climate (management commitment and involvement) can be significantly, positively influenced by successful 5S events, which may translate to improved safety overall. Originality/value – Prior literature on 5S speculates a positive impact of 5S on safety and safety climate, but this assertion is not well supported with empirical evidence. This study provides quantitative measurement of positive safety climate changes that resulted from a successful 5S event. The results provide additional incentive for management to continue 5S and other Lean activities with the possibility of also improving safety.
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Guinta, D. R., and L. J. Korn. "Differential order of replication of Xenopus laevis 5S RNA genes." Molecular and Cellular Biology 6, no. 7 (July 1986): 2536–42. http://dx.doi.org/10.1128/mcb.6.7.2536.
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In Xenopus laevis there are two multigene families of 5S RNA genes: the oocyte-type 5S RNA genes which are expressed only in oocytes and the somatic-type 5S RNA genes which are expressed throughout development. The Xenopus 5S RNA replication-expression model of Gottesfeld and Bloomer (Cell 28:781-791, 1982) and Wormington et al. (Cold Spring Harbor Symp. Quant. Biol. 47:879-884, 1983) predicts that the somatic-type 5S RNA genes replicate earlier in the cell cycle than do the oocyte-type genes. Hence, the somatic-type 5S RNA genes have a competitive advantage in binding the transcription factor TFIIIA in somatic cells and are thereby expressed to the exclusion of the oocyte-type genes. To test the replication-expression model, we determined the order of replication of the oocyte- and somatic-type 5S RNA genes. Xenopus cells were labeled with bromodeoxyuridine, stained for DNA content, and then sorted into fractions of S phase by using a fluorescence-activated cell sorter. The newly replicated DNA containing bromodeoxyuridine was separated from the lighter, unreplicated DNA by equilibrium centrifugation and was hybridized with DNA probes specific for the oocyte- and somatic-type 5S RNA genes. In this way we found that the somatic-type 5S RNA genes replicate early in S phase, whereas the oocyte-type 5S RNA genes replicate late in S phase, demonstrating a key aspect of the replication-expression model.
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DiBarra, Camilla. "5S—A Tool for Culture Change in Shipyards." Journal of Ship Production 18, no. 03 (August 1, 2002): 143–51. http://dx.doi.org/10.5957/jsp.2002.18.3.143.
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5S is a successful education/change management program that has been used in many industries to institute a culture of orderliness in the workplace. 5S programs are common in the automotive, aerospace and many other industries. The U.S. shipbuilding and repair industry has recently embraced 5S objectives despite the fact that shipyards view their operations as unique from other industries. A possible reason for this decision is the culture change leverage that a 5S program can provide. The elements of 5S that make it a powerful change program that is effective in a wide variety of industries are: simplicity of concept, commonsense approach, creation of a common purpose, employee driven changes, principle-based ideas, focused events, a structure for sustaining and structured continuous improvement. The author provides examples of specific 5S applications in ship repair as well as a discussion of the elements that make 5S an effective culture change tool.
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Devani, Vera. "Analisis Penerapan Konsep 5S di Bagian Proses Maintenance PT. Traktor Nusantara." Jurnal Teknik Industri: Jurnal Hasil Penelitian dan Karya Ilmiah dalam Bidang Teknik Industri 2, no. 2 (December 1, 2016): 113. http://dx.doi.org/10.24014/jti.v2i2.5095.
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Tujuan dari penelitian iniadalah untuk mengetahui persentase tanggapan positif dan negatif karyawan terhadap implementasi 5S, mengetahui masalah–masalah dalam penerapan 5S pada bagian proses maintenance, serta untuk menentukan proses pengendalian dan tindak lanjut penerapan 5S pada bagian proses maintenance di PT. Traktor Nusantara Pekanbaru. Metode yang digunakan dalam penelitian ini adalah Metode 5S. 5S adalah teknik untuk menjaga mutu lingkungan sebuah perusahaan atau institusi dengan cara mengembangkan keterorganisirannya, perlunya tempat kerja yang aman dan nyaman, pengelolaan tempat kerja, dan pentingnya peningkatan efisiensi dan produktifitas. Responden pada penelitian ini adalah seluruh mekanik yang bekerja pada bagian maintenance PT. Traktor Nusantara Pekanbaru. Hasil pengolahan data terhadap konsep 5S secara keseluruhan indikator, dapat diketahui bahwa rata-rata yaitu sebanyak 84,91% responden memberikan tanggapan positif. Hal ini mengindikasikan bahwa pada umumnya mekanik PT. Traktor Nusantara sudah baik dalam memahami dan mengaplikasikan konsep 5S. Namun masih terdapat sekelompok mekanik yang memiliki respon negatif yaitu sebesar 15,08%. Masalah-masalah dalam penerapan 5S, mekanik kurang optimal dalam hal menerapkan prinsip-prinsip Seiketsu (pemantapan). Untuk mengatasi hal tersebut perlu dilakukan pengkodean peralatan dan mengaruskan setiap karyawan untuk mengembalikan perlengkapan kerja yang sudah digunakan ketempat asal.
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Prawira, Atma Yudha, Yuwarni Rahayu, Mohammad Hamsal, and Humiras Hardi Purba. "A Case Study: How 5S Implementation Improves Productivity of Heavy Equipment in Mining Industry." Independent Journal of Management & Production 9, no. 4 (December 1, 2018): 1184. http://dx.doi.org/10.14807/ijmp.v9i4.826.
Full textAbstract:
This research aims to identify and present key concepts of 5S perspective. These findings link 5S to productivity improvement, which are aligned to an integrated maintenance system rather than maintenance system before. Data were collected from one of mining company in Indonesia. The data is concern in downtime unit, availability and productivity from one of heavy equipment, which is huge dumb truck. Then applying 5S method to decrease downtime unit, increase availability and at the end productivity of heavy equipment is increasing. The result will be compared between before and after 5S implementation. These findings demonstrate the importance of both the technical (visible) and philosophical (invisible) approaches required for each of the 5S components and are discussed in a team rather than cultural framework. The results indicate that 5S implementation may be a source of competitive advantage which can increase heavy equipment performance. The originality and value of the paper is come from the general understanding of the application of 5S and its use as an improvement tool at the system or process level. 5S within the context identified is the strategic platform for the managerial decisions required for the development of an integrated maintenance system.
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Morzycka-Wroblewska, E., E. U. Selker, J. N. Stevens, and R. L. Metzenberg. "Concerted evolution of dispersed Neurospora crassa 5S RNA genes: pattern of sequence conservation between allelic and nonallelic genes." Molecular and Cellular Biology 5, no. 1 (January 1985): 46–51. http://dx.doi.org/10.1128/mcb.5.1.46-51.1985.
Full textAbstract:
About 100 genes coding for 5S RNA in Neurospora crassa are dispersed throughout the genome (Selker et al., Cell 24:815-818, 1981; R. L. Metzenberg, J. N. Stevens, E. U. Selker, and E. Morzycka-Wroblewska, manuscript in preparation). The majority of them correspond to the most abundant species (alpha) of 5S RNA found in the cell. Gene conversion, gene transposition, or both may be responsible for the maintenance of sequence homogeneity (concerted evolution) of alpha-type 5S genes. To explore these possibilities, we isolated and characterized separate 5S regions from two distantly related laboratory strains of N. crassa. Restriction and sequence analyses revealed no differences in molecular location of allelic 5S genes between the two strains. However, the DNA sequences around the 5S genes are ca. 10% divergent. We concluded that transposition is not frequent enough to account for the concerted evolution of N. crassa alpha-5S genes. In contrast to sequence divergence in the flanking regions between the two strains, the 5S transcribed regions are identical (with one exception), suggesting that these genes are being corrected. We have found that flanking sequences of various N. crassa 5S genes within each strain are largely different. Thus, if the correction mechanism is based on gene conversion, it is limited to the transcribed regions of the genes. However, we did find a short region of consensus including the sequence TATA located 25 to 30 nucleotides preceding the position of transcription initiation. This region may be involved in the transcription of N. crassa 5S genes.
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Morzycka-Wroblewska, E., E. U. Selker, J. N. Stevens, and R. L. Metzenberg. "Concerted evolution of dispersed Neurospora crassa 5S RNA genes: pattern of sequence conservation between allelic and nonallelic genes." Molecular and Cellular Biology 5, no. 1 (January 1985): 46–51. http://dx.doi.org/10.1128/mcb.5.1.46.
Full textAbstract:
About 100 genes coding for 5S RNA in Neurospora crassa are dispersed throughout the genome (Selker et al., Cell 24:815-818, 1981; R. L. Metzenberg, J. N. Stevens, E. U. Selker, and E. Morzycka-Wroblewska, manuscript in preparation). The majority of them correspond to the most abundant species (alpha) of 5S RNA found in the cell. Gene conversion, gene transposition, or both may be responsible for the maintenance of sequence homogeneity (concerted evolution) of alpha-type 5S genes. To explore these possibilities, we isolated and characterized separate 5S regions from two distantly related laboratory strains of N. crassa. Restriction and sequence analyses revealed no differences in molecular location of allelic 5S genes between the two strains. However, the DNA sequences around the 5S genes are ca. 10% divergent. We concluded that transposition is not frequent enough to account for the concerted evolution of N. crassa alpha-5S genes. In contrast to sequence divergence in the flanking regions between the two strains, the 5S transcribed regions are identical (with one exception), suggesting that these genes are being corrected. We have found that flanking sequences of various N. crassa 5S genes within each strain are largely different. Thus, if the correction mechanism is based on gene conversion, it is limited to the transcribed regions of the genes. However, we did find a short region of consensus including the sequence TATA located 25 to 30 nucleotides preceding the position of transcription initiation. This region may be involved in the transcription of N. crassa 5S genes.
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Linares, Concha, Juan González, Esther Ferrer, and Araceli Fominaya. "The use of double fluorescence in situ hybridization to physically map the positions of 5S rDNA genes in relation to the chromosomal location of 18S–5.8S–26S rDNA and a C genome specific DNA sequence in the genus Avena." Genome 39, no. 3 (June 1, 1996): 535–42. http://dx.doi.org/10.1139/g96-068.
Full textAbstract:
A physical map of the locations of the 5S rDNA genes and their relative positions with respect to 18S–5.8S–26S rDNA genes and a C genome specific repetitive DNA sequence was produced for the chromosomes of diploid, tetraploid, and hexaploid oat species using in situ hybridization. The A genome diploid species showed two pairs of rDNA loci and two pairs of 5S loci located on both arms of one pair of satellited chromosomes. The C genome diploid species showed two major pairs and one minor pair of rDNA loci. One pair of subtelocentric chromosomes carried rDNA and 5S loci physically separated on the long arm. The tetraploid species (AACC genomes) arising from these diploid ancestors showed two pairs of rDNA loci and three pairs of 5S loci. Two pairs of rDNA loci and 2 pairs of 5S loci were arranged as in the A genome diploid species. The third pair of 5S loci was located on one pair of A–C translocated chromosomes using simultaneous in situ hybridization with 5S rDNA genes and a C genome specific repetitive DNA sequence. The hexaploid species (AACCDD genomes) showed three pairs of rDNA loci and six pairs of 5S loci. One pair of 5S loci was located on each of two pairs of C–A/D translocated chromosomes. Comparative studies of the physical arrangement of rDNA and 5S loci in polyploid oats and the putative A and C genome progenitor species suggests that A genome diploid species could be the donor of both A and D genomes of polyploid oats. Key words : oats, 5S rDNA genes, 18S–5.8S–26S rDNA genes, C genome specific repetitive DNA sequence, in situ hybridization, genome evolution.
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Chang, Yung-Chia, and Chuan-Yung Chen. "Prioritisation on 5S activities for a semiconductor wafer fabrication: an empirical study." International Journal of Quality & Reliability Management 31, no. 4 (April 1, 2014): 380–94. http://dx.doi.org/10.1108/ijqrm-01-2012-0003.
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Purpose – Semiconductor wafer fabrication (FAB) is recognized as one of the most complex manufacturing systems. A newly built FAB has to pass various audits from its customer before the customer's wafers are initially produced. 5S audit is one of them. In order to comply with customer (auditor) expectations toward 5S practice, this paper assists wafer fabrication managers in allocating the limited resources to places that are valued most by their customer. The paper aims to discuss these issues. Design/methodology/approach – This paper applied Yang's refined Kano model to Ho's 5S checklist to prioritise these checkpoints for a FAB. An empirical study based on Ho's 5S checklist from experienced internal auditors (respondents) to prioritise 5S activities was explored to justify its feasibility for a FAB and importance-satisfaction for customer. Findings – An empirical study in a case FAB demonstrated how the refined model prioritised 5S activities based on Ho's 5S checklist. The result of this study further showed that quality attributes possess different identities, which could offer management more framable scopes to implement 5S practice and sustain 5S scene management. Research limitations/implications – Since this empirical study was focused on a 300-mm wafer fabrication company, the results and findings may not generally explain other wafer-size fabrications. Practical implications – This study was applied to a real-world case of a newly built 300-mm semiconductor fabrication in Taiwan. It is a straightforward bridge to link a methodology in a practical manner to disseminate information to both researchers and practitioners. Originality/value – From the adoption of the refined Kano model, specific required check points for 5S practice are transformed from subjective, conceptual and linguistic practice to be identified, quantified and prioritised for semiconductor wafer fabrication under resources constraints to cater customer's 5S expectations and to generate more attention in building-up a much more robust scene management. This paper provided a systemic way to prioritise 5S activities for a semiconductor wafer fabrication.
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Singh, Kapil, Sabhyata Bhatia, and Malathi Lakshmikumaran. "Novel variants of the 5S rRNA genes in Eruca sativa." Genome 37, no. 1 (February 1, 1994): 121–28. http://dx.doi.org/10.1139/g94-015.
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The 5S ribosomal RNA (rRNA) genes of Eruca sativa were cloned and characterized. They are organized into clusters of tandemly repeated units. Each repeat unit consists of a 119-bp coding region followed by a noncoding spacer region that separates it from the coding region of the next repeat unit. Our study reports novel gene variants of the 5S rRNA genes in plants. Two families of the 5S rDNA, the 0.5-kb size family and the l-kb size family, coexist in the E. sativa genome. The 0.5-kb size family consists of the 5S rRNA genes (S4) that have coding regions similar to those of other reported plant 5S rDNA sequences, whereas the 1-kb size family consists of the 5S rRNA gene variants (S1) that exist as 1-kb BamHI tandem repeats. S1 is made up of two variant units (V1 and V2) of 5S rDNA where the BamHI site between the two units is mutated. Sequence heterogeneity among S4, V1, and V2 units exists throughout the sequence and is not limited to the noncoding spacer region only. The coding regions of V1 and V2 show approximately 20% dissimilarity to the coding regions of S4 and other reported plant 5S rDNA sequences. Such a large variation in the coding regions of the 5S rDNA units within the same plant species has been observed for the first time. Restriction site variation is observed between the two size classes of 5S rDNA in E. sativa. The noncoding spacers of the variants V1 and V2 that make up the 1-kb family lack the EcoRI site that is present in the 0.5-kb family. The sequence analysis indicates that V1 and V2 sequences are probably pseudogenes derived from functional 5S rRNA genes. The results also suggest that the two families exist as independent clusters at different locations in the E. sativa genome.Key words: 5S rRNA genes, crucifers, Eruca sativa, organization, sequence analysis.