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1
Wahyudin, Agus, Y. Yuwariah, Fiky Yulianto W, and A. F. Kevin A. "Respons Tanaman Jagung (Zea mays L.) Hibrida Akibat Jarak Tanam Berbeda Pada Sistem Tatam Legowo (2:1) Dan Jenis Pupuk Organik Di Inceptisols Jatinangor." Paspalum: Jurnal Ilmiah Pertanian 6, no. 1 (April 1, 2018): 20. http://dx.doi.org/10.35138/paspalum.v6i1.74.
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The objective of this research was to find the best combination of plant spacing and organic fertilizer on the growth and yield of maize (Zea mays L.) Pertiwi-3 in Jatinangor. The experiment was carried outin Ciparanje experimental field on November 2016 to March 2017. The experiment used was Randomized Block Design (RBD) which consisted of six treatments and four replications, which were the plant spacing 20cmx20xmx75cm + 5t/ha compost, 25cmx25cmx75cm + 5t/ha compost, 30cmx30cmx75cm + 5t/ha compost, 20cmx20cmx75cm + 5t/ha cow manure, 25cmx25cmx75cm + 5t/ha cow manure and 30cmx30cmx75cm + 5t/ha cow manure. The result of the experiment showed that the best result of 100 dry seeds weight was in the treatment of plant spacing 20cmx20cmx75cm + 5t/ha cow manure with a weight of 45,19g. The highest score of Leaf Area Index (LAI) was in the treatment of plant spacing 20cmx20cmx75cm + 5t/ha cow manure score of 2,92 was not significantly different from the treatment of plant spacing 20cmx20cmx75cm + 5t/ha compost with score of 2,82.
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Kim, Young-Ok, Hee Jeong Kong, Sooyeon Park, So-Jung Kang, Kyung-Kil Kim, Dae Yeon Moon, Tae-Kwang Oh, and Jung-Hoon Yoon. "Paracoccus fistulariae sp. nov., a lipolytic bacterium isolated from bluespotted cornetfish, Fistularia commersonii." International Journal of Systematic and Evolutionary Microbiology 60, no. 12 (December 1, 2010): 2908–12. http://dx.doi.org/10.1099/ijs.0.021808-0.
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A Gram-stain-negative, non-motile, non-spore-forming and short rod- or rod-shaped bacterial strain, designated 22-5T, was isolated from a bluespotted cornetfish, Fistularia commersonii, and subjected to taxonomic study. Strain 22-5T grew optimally at 30 °C and in the presence of 2–5 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 22-5T belonged to the genus Paracoccus and joined the cluster comprising Paracoccus homiensis DD-R11T and Paracoccus zeaxanthinifaciens ATCC 21588T, with which strain 22-5T exhibited 97.4 and 96.9 % 16S rRNA gene sequence similarity, respectively. Strain 22-5T exhibited 94.0–96.6 % 16S rRNA gene sequence similarity with the other type strains of species of the genus Paracoccus. Strain 22-5T contained Q-10 as the predominant menaquinone and C18 : 1 ω7c as the predominant fatty acid. In this study, P. zeaxanthinifaciens KCTC 22688T also contained Q-10 as the predominant isoprenoid quinone. The DNA G+C content of strain 22-5T was 63.6 mol%. Strain 22-5T exhibited 44 and 32 % DNA–DNA relatedness to P. homiensis KACC 11518T and P. zeaxanthinifaciens KCTC 22688T, respectively. On the basis of phenotypic, phylogenetic and genetic data, strain 22-5T is considered to represent a novel species of the genus Paracoccus, for which the name Paracoccus fistulariae sp. nov. is proposed. The type strain is 22-5T (=KCTC 22803T =CCUG 58401T).
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Mantovani, Vilma, Paolo Garagnani, Paola Selva, Cesare Rossi, Simona Ferrari, Marinella Cenci, Nilla Calza, Vincenzo Cerreta, Donata Luiselli, and Giovanni Romeo. "Simple Method for Haplotyping the Poly(TG) Repeat in Individuals Carrying the IVS8 5T Allele in the CFTR Gene." Clinical Chemistry 53, no. 3 (March 1, 2007): 531–33. http://dx.doi.org/10.1373/clinchem.2006.074807.
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Abstract Background: The 5T allele of the polyT tract located within intron 8 of the cystic fibrosis transmembrane conductance regulator (CFTR) gene is a variant that in trans with a severe CFTR mutation can result in normal phenotype, congenital bilateral absence of vas deferens (CBAVD), or mild cystic fibrosis. The 5T allele has been associated with the skipping of exon 9, a process that seems to be influenced by an adjacent 9–13TG tandem repeat. The 12- or 13TG repeats are often associated with an abnormal phenotype. We present here a single-step method for direct haplotyping of the TG repeats in 5T carriers. Method: The method is based on a single-step PCR, using a fluorescently labeled forward primer and a reverse allele-specific primer matching the 5T allele. We validated the test in 30 control samples of known 5T-poly(TG) haplotype and then used this method to evaluate 57 clinical samples. Results: The expected TG genotypes were obtained for all 5T control samples, and no nonspecific amplification of either the 7T or 9T alleles was detected. In our 5T-positive collection 9 of 9 (100%) CBAVD patients, 6 of 12 (50.0%) chronic pancreatitis patients, and 12 of 36 (33.3%) individuals undergoing assisted reproduction showed 5T-12TG haplotype. Conclusions: Our method is an accurate, specific, and simple tool to characterize the 5T poly(TG) haplotype. Our results confirm the high frequency of 5T-12TG in CBAVD patients and do not preclude a potential effect also in pancreatitis. This assay can be useful in assessment of the disease risk in 5T carriers.
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Yang, He, Jian Hai Yue, and Jian Yan. "5T Information Fusion System Based on Train Technology Scheme Design." Applied Mechanics and Materials 599-601 (August 2014): 1229–32. http://dx.doi.org/10.4028/www.scientific.net/amm.599-601.1229.
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On the train parts of 5T system at present all kinds of fault real-time monitoring the relevance of the weak, poor information sharing, centralized monitoring problem not in time, the system can achieve 5T effective integration of information system, information real-time delivery, 5T information fusion and 5T information fusion integrated query and statistical analysis and other functions through the acquisition of 5T system data, to train vehicle as the object of management, in order to train car number as the only connection identifier, fusion 5T information resources, to establish a global view of train vehicle safety monitoring information model. To improve the level of security early warning and fault analysis ability is of great significance.
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SIAD, M. B., Z. EL JOUAD, A. KHELIL, A. MOHAMMED KRARROUBI, S. MORSLI, G. NECULQUEO, M. ADDOU, J. C. BERNÈDE, and L. CATTIN. "COMPARISON OF PERFORMANCES OF ORGANIC PHOTOVOLTAIC CELLS USING SubPc AS CENTRAL AMBIPOLAR LAYER IN TERNARY STRUCTURES AND AS ELECTRON ACCEPTOR IN BINARY STRUCTURES." Surface Review and Letters 27, no. 07 (December 11, 2019): 1950184. http://dx.doi.org/10.1142/s0218625x19501841.
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We compare the performances of organic photovoltaic cells (OPVCs) based on binary and ternary planar heterojunctions. The organic active layers are pentathiophene (5T), subphthalocyanine (SubPc) and fullerene (C60). SubPc being an ambipolar material we used it either as electron acceptor in binary OPVCs or as central layer in ternary cells in order to increase the efficiency of OPVCs using 5T as electron donor. So, the different OPVC configurations were 5T/C60, 5T/SubPc and 5T/SubPc/C60. The effect of the different organic layer thicknesses on the device performances was studied. In order to understand the behavior of the different OPVC configurations, we proceeded with a morphological study. The influence of the high roughness of the 5T layer on the OPVCs performances is discussed. The best OPVCs performances are obtained with the binary structure 5T/SubPc. Its maximum efficiency corresponds to an increase of 50% compared to the OPVC based on the couple 5T/C60. External Quantum Efficiency measurements show that both layers participate to the current generation. The efficiency increase is mainly due to the increase of the open circuit voltage ([Formula: see text]). In the case of ternary OPVCs, [Formula: see text] is limited by the band structure of 5T and C60, moreover, the efficiency is also limited by the poor charge collection efficiency of the ternary structure and the series resistance of the three stacked organic layers.
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Yoon, Jung-Hoon, So-Jung Kang, Soo-Young Lee, Jung-Sook Lee, and Tae-Kwang Oh. "Kangiella geojedonensis sp. nov., isolated from seawater." International Journal of Systematic and Evolutionary Microbiology 62, Pt_3 (March 1, 2012): 511–14. http://dx.doi.org/10.1099/ijs.0.029314-0.
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A Gram-stain-negative, non-motile, non-spore-forming bacterial strain, YCS-5T, was isolated from seawater off the southern coast of Korea. Strain YCS-5T grew optimally at 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain YCS-5T fell within the clade comprising Kangiella species. Strain YCS-5T exhibited 16S rRNA gene sequence similarity values of 96.6, 95.7 and 97.9 % to the type strains of Kangiella koreensis, Kangiella aquimarina and Kangiella japonica, respectively, and less than 89.8 % to strains of other species used in the phylogenetic analysis. Strain YCS-5T contained Q-8 as the predominant ubiquinone and iso-C17 : 0, iso-C15 : 0, iso-C11 : 0 3-OH and iso-C17 : 1ω9c as the major fatty acids. The polar lipid profile of strain YCS-5T was similar to that of K. koreensis SW-125T, with phosphatidylglycerol and an unidentified aminolipid as major polar lipids. The DNA G+C content was 47 mol%. The mean DNA–DNA relatedness value between strain YCS-5T and K. japonica JCM 16211T was 12 %. Differential phenotypic properties and the phylogenetic and genetic distinctiveness of strain YCS-5T demonstrated that this strain is distinguishable from other Kangiella species. On the basis of the data presented, strain YCS-5T is considered to represent a novel species of the genus Kangiella, for which the name Kangiella geojedonensis sp. nov. is proposed; the type strain is YCS-5T ( = KCTC 23420T = CCUG 60526T).
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Yoon, Jung-Hoon, Yong-Taek Jung, and Jung-Sook Lee. "Loktanella litorea sp. nov., isolated from seawater." International Journal of Systematic and Evolutionary Microbiology 63, Pt_1 (January 1, 2013): 175–80. http://dx.doi.org/10.1099/ijs.0.039198-0.
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A Gram-negative, non-motile, rod-shaped bacterial strain, designated DPG-5T, was isolated from seawater of the South Sea, Korea and subjected to a study using a polyphasic taxonomic approach. Strain DPG-5T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. Neighbour-joining phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DPG-5T fell within the clade comprising members of the genus Loktanella , and formed a cluster with the type strains of Loktanella rosea , Loktanella maricola , Loktanella koreensis and Loktanella tamlensis , with which it exhibited highest 16S rRNA gene sequence similarity values of 96.7, 96.5, 96.2 and 96.7 %, respectively. The 16S rRNA gene sequence similarity values between strain DPG-5T and the type strains of the other species of the genus Loktanella were in the range of 94.4–96.0 %. The DNA G+C content of strain DPG-5T was 57.6 mol%. Strain DPG-5T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c and 11-methyl C18 : 1ω7c as the major fatty acids. The major polar lipids found in strain DPG-5T were phosphatidylcholine and phosphatidylglycerol. Differential phenotypic properties, together with the phylogenetic distinctiveness, showed that strain DPG-5T is differentiated from other species of the genus Loktanella . On the basis of the data presented, strain DPG-5T is considered to represent a novel species of the genus Loktanella , for which the name Loktanella litorea sp. nov. is proposed. The type strain is DPG-5T ( = KCTC 23883T = CCUG 62113T).
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He, Hairong, Chongxi Liu, Junwei Zhao, Wenjun Li, Tong Pan, Lingyu Yang, Xiangjing Wang, and Wensheng Xiang. "Streptomyces zhaozhouensis sp. nov., an actinomycete isolated from candelabra aloe (Aloe arborescens Mill)." International Journal of Systematic and Evolutionary Microbiology 64, Pt_4 (April 1, 2014): 1096–101. http://dx.doi.org/10.1099/ijs.0.056317-0.
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A novel endophytic actinomycete, designated strain NEAU-LZS-5T, was isolated from the leaf of candelabra aloe (Aloe arborescens Mill) and characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that strain NEAU-LZS-5T belongs to the genus Streptomyces and exhibited 99.51 and 97.37 % similarity to Streptomyces sedi YIM 65188T and Streptomyces specialis GW41-1564T, respectively, whereas low similarity values (<97 %) distinguished strain NEAU-LZS-5T from all other species of the genus Streptomyces with validly published names. Two tree-making algorithms also supported the position that strain NEAU-LZS-5T formed a distinct clade with Streptomyces sedi YIM 65188T and Streptomyces specialis GW41-1564T. However, levels of DNA–DNA relatedness between strain NEAU-LZS-5T and Streptomyces sedi YIM 65188T and Streptomyces specialis GW41-1564T were 45.59 and 31.90 %, respectively. A comparative study between strain NEAU-LZS-5T and the type strains of closest related species of the genus Streptomyces revealed that it differed from them in morphological, physiological and biochemical characteristics. Therefore, strain NEAU-LZS-5T represents a novel species of the genus Streptomyces , for which the name Streptomyces zhaozhouensis sp. nov. is proposed. The type strain is NEAU-LZS-5T ( = CGMCC 4.7095T = DSM 42101T).
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Park, Sooyeon, So-Jung Kang, Tae-Kwang Oh, and Jung-Hoon Yoon. "Roseivivax lentus sp. nov., isolated from a tidal flat sediment, and emended description of the genus Roseivivax Suzuki et al. 1999." International Journal of Systematic and Evolutionary Microbiology 60, no. 5 (May 1, 2010): 1113–17. http://dx.doi.org/10.1099/ijs.0.014795-0.
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A Gram-negative-staining, aerobic, non-motile and rod-shaped bacterial strain, S5-5T, was isolated from a tidal flat sediment at Saemankum on the west coast of Korea and subjected to a polyphasic taxonomic investigation. Strain S5-5T grew optimally at pH 7.5–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. It did not produce bacteriochlorophyll a. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain S5-5T is phylogenetically closely related to the genus Roseivivax, joining the cluster comprising the two recognized Roseivivax species. The 16S rRNA gene sequence similarity between strain S5-5T and members of the genus Roseivivax was in the range 95.0–96.7 %. Strain S5-5T contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c and 11-methyl C18 : 1 ω7c as the major fatty acids. The DNA G+C content was 68.2 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain S5-5T could be differentiated from Roseivivax species. On the basis of the data presented, strain S5-5T is considered to represent a novel species of the genus Roseivivax, for which the name Roseivivax lentus sp. nov. is proposed. The type strain is S5-5T (=KCTC 22708T =CCUG 57755T).
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Shen, Liang, Yongqin Liu, Zhengquan Gu, Tandong Yao, Baiqing Xu, Ninglian Wang, Nianzhi Jiao, Hongcan Liu, and Yuguang Zhou. "Arcticibacter eurypsychrophilus sp. nov., isolated from ice core." International Journal of Systematic and Evolutionary Microbiology 65, Pt_2 (February 1, 2015): 639–43. http://dx.doi.org/10.1099/ijs.0.066365-0.
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A Gram-stain-negative, rod-shaped, non-flagellated bacterium, strain MJ9-5T, was isolated from ice core of Muji Glacier. Colonies of strain MJ9-5T were pink, convex and round on R2A agar. Strain MJ9-5T grew between −1 to 25 °C with an optimum growth temperature of 10–15 °C. The strain tolerated 0–1.2 % (w/v) NaCl with an optimum of 1 %. The major cellular fatty acids of strain MJ9-5T were iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C1 6 : 1ω7c). The G+C content of the genomic DNA was 38.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ9-5T was related to members of the genus Arcticibacter . On the basis of the phenotypic characteristics and phylogenetic analysis, a novel species of this genus, Arcticibacter eurypsychrophilus sp. nov., is proposed. The type strain is MJ9-5T ( = KCTC 42008T = JCM 19862T).
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Iino, Takao, Koji Mori, Takashi Itoh, Takuji Kudo, Ken-ichiro Suzuki, and Moriya Ohkuma. "Description of Mariniphaga anaerophila gen. nov., sp. nov., a facultatively aerobic marine bacterium isolated from tidal flat sediment, reclassification of the Draconibacteriaceae as a later heterotypic synonym of the Prolixibacteraceae and description of the family Marinifilaceae fam. nov." International Journal of Systematic and Evolutionary Microbiology 64, Pt_11 (November 1, 2014): 3660–67. http://dx.doi.org/10.1099/ijs.0.066274-0.
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A mesophilic, chemoheterotrophic bacterium, strain Fu11-5T, was isolated from tidal-flat sediment from Tokyo Bay, Chiba, Japan. Cells of strain Fu11-5T were facultatively aerobic, Gram-negative, non-sporulating, non-motile and rod-shaped (1.9–6.9 µm long). Strain Fu11-5T grew optimally at 35–37 °C and pH 6.5–7.0 and with 1–2 % (w/v) NaCl. Oxygen and l-cysteine were used as an alternative electron acceptor and donor, respectively. Strain Fu11-5T also grew fermentatively on some pentoses, hexoses and disaccharides and soluble starch. Succinic acid was the major end product from d-glucose. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain Fu11-5T was affiliated with the order Bacteroidales , and its nearest neighbours were members of the genera Meniscus , Prolixibacter , Sunxiuqinia , Mangrovibacterium and Draconibacterium, with 87–91 % sequence similarity. Cell morphology, optimum growth temperature and utilization of sugars of strain Fu11-5T distinguished the strain from phylogenetically related bacteria. On the basis of its phenotypic features and phylogenetic position, a novel genus and species are proposed to accommodate strain Fu11-5T, with the name Mariniphaga anaerophila gen. nov., sp. nov. The type strain of Mariniphaga anaerophila is strain Fu11-5T ( = JCM 18693T = NBRC 109408T = DSM 26910T). We also propose to combine the family Draconibacteriaceae into the family Prolixibacteraceae as a later heterotypic synonym and to place the distinct sublineage of the genus Marinifilum in the family Marinifilaceae fam. nov.
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Kim, Young-Ok, Sooyeon Park, Bo-Hye Nam, Ji-Min Park, Dong-Gyun Kim, and Jung-Hoon Yoon. "Roseovarius scapharcae sp. nov., isolated from ark shell Scapharca broughtonii." International Journal of Systematic and Evolutionary Microbiology 65, Pt_12 (December 1, 2015): 4695–700. http://dx.doi.org/10.1099/ijsem.0.000633.
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A Gram-stain-negative, non-motile, aerobic and ovoid or rod-shaped bacterium, designated MA4-5T, was isolated from ark shell (Scapharca broughtonii) collected from the South Sea, South Korea. The novel strain grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees based on 16S rRNA gene sequences showed that strain MA4-5T forms a coherent cluster with the type strains of Roseovarius albus, Roseovarius aestuarii and Roseovarius nubinhibens, sharing 97.0–99.2 % sequence similarity. It exhibited 16S rRNA gene sequence similarity of 93.1–96.1 % to the type strains of other Roseovarius species. Strain MA4-5T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain MA4-5T were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain MA4-5T was 53.8 mol% and its mean DNA–DNA relatedness values with the type strains of R. albus, R. aestuarii and R. nubinhibens were 11–26 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, indicated that strain MA4-5T is separate from recognized species of the genus Roseovarius. On the basis of the data presented, strain MA4-5T is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius scapharcae sp. nov. is proposed. The type strain is MA4-5T ( = KCTC 42703T = NBRC 111226T).
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Shimane, Yasuhiro, Shuhei Nagaoka, Hiroaki Minegishi, Masahiro Kamekura, Akinobu Echigo, Yuji Hatada, Takashi Ito, and Ron Usami. "Natronoarchaeum philippinense sp. nov., a haloarchaeon isolated from commercial solar salt." International Journal of Systematic and Evolutionary Microbiology 63, Pt_3 (March 1, 2013): 920–24. http://dx.doi.org/10.1099/ijs.0.042549-0.
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A Gram-staining-negative, pleomorphic, aerobic, halophilic archaeon, designated strain 294-194-5T, was isolated in Japan from commercial solar salt imported from the Philippines. Colonies of strain 294-194-5T were translucent and red. Strain 294-194-5T was able to grow at 20–50 °C (optimum, 37–45 °C), with 14–30 % (w/v) NaCl (optimum, 18 %), and at pH 6.5–8.5 (optimum, pH 8.0). MgCl2 was not required for growth. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain 294-194-5T was most closely related to Natronoarchaeum mannanilyticum YSM-123T (96.8–97.1 % sequence similarities). The major polar lipids of the novel strain were the C20C20 and C20C25 derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester and the same glycolipids (disulfated diglycosyl diether and one unidentified glycolipid) as detected in N. mannanilyticum YSM-123T. The DNA G+C content of strain 294-194-5T was 63.0 mol%. The DNA–DNA relatedness values between the novel strain and N. mannanilyticum YSM-123Twere 46.5 % and 48.5 % (reciprocal). Based on these data, strain 294-194-5T represents a novel species of the genus Natronoarchaeum , for which the name Natronoarchaeum philippinense sp. nov. is proposed. The type strain is 294-194-5T ( = JCM 16593T = CECT 7630T).
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Nunoura, Takuro, Hanako Oida, Masayuki Miyazaki, Yohey Suzuki, Ken Takai, and Koki Horikoshi. "Marinitoga okinawensis sp. nov., a novel thermophilic and anaerobic heterotroph isolated from a deep-sea hydrothermal field, Southern Okinawa Trough." International Journal of Systematic and Evolutionary Microbiology 57, no. 3 (March 1, 2007): 467–71. http://dx.doi.org/10.1099/ijs.0.64640-0.
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A novel thermophilic and sulfur-reducing heterotrophic bacterium, strain TFS10-5T, was isolated from a deep-sea hydrothermal field in Yonaguni Knoll IV, Southern Okinawa Trough. Cells of strain TFS10-5T were motile rods, 1.5–5 μm in length and 0.5–0.8 μm in width. Strain TFS10-5T was an obligately anaerobic heterotroph and sulfur-reduction stimulated growth. Growth was observed between 30 and 70 °C (optimum at 55–60 °C), pH 5.0–7.4 (optimum at pH 5.5–5.8), 1.0–5.5 NaCl % (optimum at 3.0–3.5 %). The fatty acid content was C16 : 0 (71.0 %), C16 : 1 (6.0 %), C18 : 0 (21.4 %) and C18 : 1 (1.6 %). The G+C content of the genomic DNA was 28 mol%. 16S rRNA gene sequence analysis indicated that strain TFS10-5T belongs to the genus Marinitoga. Based on the physiological and phylogenetic features of the new isolate, strain TFS10-5T represents a novel species in the genus Marinitoga for which the name Marinitoga okinawensis sp. nov. is proposed. The type strain is TFS10-5T (=JCM 13303T=DSM 17373T).
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Lee, Keun Chul, Kwang Kyu Kim, Jong-Shik Kim, Dae-Shin Kim, Suk-Hyung Ko, Seung-Hoon Yang, and Jung-Sook Lee. "Cohnella collisoli sp. nov., isolated from lava forest soil." International Journal of Systematic and Evolutionary Microbiology 65, Pt_9 (September 1, 2015): 3125–30. http://dx.doi.org/10.1099/ijsem.0.000388.
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A novel bacterial strain, NKM-5T, was isolated from soil of a lava forest in Nokkome Oreum, Jeju, Republic of Korea. Cells of strain NKM-5T were Gram-stain-positive, motile, endospore-forming, rod-shaped and oxidase- and catalase-positive. Strain NKM-5T contained anteiso-C15 : 0 and iso-C16 : 0 as the major fatty acids; menaquinone-7 (MK-7) as the predominant isoprenoid quinone; diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phosphatidylglycerol, an unidentified phospholipid and three unidentified aminophospholipids as the polar lipids; and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content was 48.3 mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain NKM-5T was most closely related to Cohnella lupini RLAHU4BT (96.9 % sequence similarity) and fell into a clade in the genus Cohnella. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain NKM-5T represents a novel species of the genus Cohnella, for which the name Cohnella collisoli sp. nov. is proposed. The type strain is NKM-5T ( = KCTC 33634T = CECT 8805T).
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Jin, Chun-Zhi, Ye Zhuo, Xuewen Wu, So-Ra Ko, Taihua Li, Feng-Jie Jin, Chi-Yong Ahn, Hee-Mock Oh, Hyung-Gwan Lee, and Long Jin. "Genomic and Metabolic Insights into Denitrification, Sulfur Oxidation, and Multidrug Efflux Pump Mechanisms in the Bacterium Rhodoferax sediminis sp. nov." Microorganisms 8, no. 2 (February 15, 2020): 262. http://dx.doi.org/10.3390/microorganisms8020262.
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This genus contains both phototrophs and nonphototrophic members. Here, we present a high-quality complete genome of the strain CHu59-6-5T, isolated from a freshwater sediment. The circular chromosome (4.39 Mbp) of the strain CHu59-6-5T has 64.4% G+C content and contains 4240 genes, of which a total of 3918 genes (92.4%) were functionally assigned to the COG (clusters of orthologous groups) database. Functional genes for denitrification (narGHJI, nirK and qnor) were identified on the genomes of the strain CHu59-6-5T, except for N2O reductase (nos) genes for the final step of denitrification. Genes (soxBXAZY) for encoding sulfur oxidation proteins were identified, and the FSD and soxF genes encoding the monomeric flavoproteins which have sulfide dehydrogenase activities were also detected. Lastly, genes for the assembly of two different RND (resistance-nodulation division) type efflux systems and one ABC (ATP-binding cassette) type efflux system were identified in the Rhodoferax sediminis CHu59-6-5T. Phylogenetic analysis based on 16S rRNA sequences and Average Nucleotide Identities (ANI) support the idea that the strain CHu59-6-5T has a close relationship to the genus Rhodoferax. A polyphasic study was done to establish the taxonomic status of the strain CHu59-6-5T. Based on these data, we proposed that the isolate be classified to the genus Rhodoferax as Rhodoferax sediminis sp. nov. with isolate CHu59-6-5T.
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Jung, Yong-Taek, Sooyeon Park, Jung-Sook Lee, and Jung-Hoon Yoon. "Erythrobacter lutimaris sp. nov., isolated from a tidal flat sediment." International Journal of Systematic and Evolutionary Microbiology 64, Pt_12 (December 1, 2014): 4184–90. http://dx.doi.org/10.1099/ijs.0.067728-0.
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A Gram-stain-negative, non-motile, coccoid- or oval-shaped bacterial strain, designated S-5T, belonging to the class Alphaproteobacteria , was isolated from a tidal flat sediment of the Yellow Sea, Korea and was subjected to a polyphasic taxonomic study. Strain S-5T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. Neighbour-joining analysis based on 16S rRNA gene sequences showed that strain S-5T fell within the clade comprising the species of the genus Erythrobacter , clustering with the type strains of Erythrobacter pelagi , Erythrobacter citreus and Erythrobacter seohaensis with which it exhibited the highest 16S rRNA gene sequence similarity (96.0–96.7 %). The DNA G+C content was 66.0 mol%. Strain S-5T contained Q-10 as the predominant ubiquinone and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C17 : 1ω6c as the major fatty acids. The major polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, an unidentified glycolipid and two unidentified lipids. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain S-5T is distinguishable from other species of the genus Erythrobacter . On the basis of the data presented, strain S-5T is considered to represent a novel species of the genus Erythrobacter , for which the name Erythrobacter lutimaris sp. nov. is proposed. The type strain is S-5T ( = KCTC 42109T = CECT 8624T).
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Xu, Lian, Hai-Tao Wang, Jin-Xin Zhang, Hui Zhang, Shuai Wang, and Ji-Quan Sun. "Flavobacterium alkalisoli sp. nov., isolated from rhizosphere soil of Suaeda salsa." International Journal of Systematic and Evolutionary Microbiology 70, no. 6 (June 1, 2020): 3888–98. http://dx.doi.org/10.1099/ijsem.0.004255.
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A Gram-negative, strictly aerobic, gliding motility, none-spore forming, yellow, rods bacterial strain, designated XS-5T, was isolated from rhizosphere soil of Suaeda salsa, in Tumd Right Banner, Inner Mongolia, PR China. A phylogenetic tree based on the 16S rRNA gene sequences and the phylogenomic tree both showed that strain XS-5T clustered with Flavobacterium beibuense F44-8T (shared 97.2 % of 16S rRNA gene similarity) and Flavobacterium rakeshii FCS-5T (97.6 %), and shared <96.0 % of 16S rRNA gene similarities with all other type strains. Strain XS-5T contained MK-6 as the major respiratory quinone. Its major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and an unidentified lipid; and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 0, iso-C15 : 0 3-OH, Summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c), and Summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl). The genome consisted of a 3 985 855 bp circular chromosome, with a G+C content of 37.9 mol%, predicting 3616 coding sequences genes, 45 tRNA genes and three rRNA operons. The average nucleotide identity, amino acid identity and digital DNA–DNA hybridization values of strain XS-5T to F. beibuense F44-8T and F. rakeshii FCS-5T were 79.2 and 79.2 %, 81.7 and 81.6 %, 22.3 and 22.2 %, respectively. The results of phylogenetic, physiological and biochemical tests allowed the discrimination of strain XS-5T from its phylogenetic relatives. Flavobacterium alkalisoli sp. nov. is therefore proposed with strain XS-5T (=CGMCC 1.17077T=KCTC 72459T) as the type strain.
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Alabi, A. S., G. S. Oladipo, B. C. Didia, E. O. Aigbogun, and K. A. Akintunde. "Homo sapiens are bilaterally symmetrical but not with toe length and toe-length ratios among Nigerians." Anatomy Journal of Africa 6, no. 3 (November 30, 2017): 1024–32. http://dx.doi.org/10.4314/aja.v6i3.163506.
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The concept of bilateral symmetry in organisms involves the division of the body plan along a plane that splits the animal's body into right and left sides that are mirror images of each other. However can this be true for toe lengths and ratios? The present study evaluates the symmetric differences in toe length and toe-length ratios among the three major ethnic groups in Nigeria. A total of 1574 adult Nigerian Hausas (664), Igbos (420) and Yorubas (490) of equal sex were included in the study. Aged between 18-65 years, were randomly selected from various states. Written informed consent was taken from each participant, sample size was determined by proportion, using Cochran formulae for infinite population. A digital Vernier caliper was used to obtain direct linear measurements of the toe length of both feet; hallux (1T), second toe (2T), third toe (3T), fourth toe (4T), and the fifth toe (5T). Ten (10) possible toe-length ratios were also determined and named as follows; 1T/2T, 1T/3T, 1T/4T, 1T/5T, 2T/3T, 2T/4T, 2T/5T, 3T/4T, 3T/5T, and 4T/5T. Data were analyzed using SPSS (IBM®Armonk, New York, USA) and Minitab V17 (Minitab® Inc. State College, Pennsylvania) statistical software. Paired-sample t-test was used to evaluate symmetry; Pearson’s Correlation was used for inter-prediction of the toes and ratios. Confidence level was set at 95%; as P-values ≤0.05 were considered significant. The result showed that some of the toe length of both feet were significantly assymetrical among the studied ethnic groups; Hausa males (1T df=1.35 and 5T df=0.55; P<0.05) and females (1T and 3T; (1T df=0.27, 3T df=0.47) P<0.001), Igbo males (1T, 3T and 4T(1T df=0.47, 3T, df=-0.53, 4T df=-0.58) ; P<0.05 and females 1T and 3T(1T df=0.40, T3 df=-0.64) ; P<0.01) and Yoruba males(1T, 2T, 3T and 5T(1T df=1.29, 2T df=0.47, 3T df=0.15, and 5T df=0.55,); P<0.001) and females (1T and 5T(1T df=0.77; 5T df=0.23) ;P<0.001). The toe-length ratios also displayed symmetrical differences for Nigerian male population; 1T:2T [t=3.78, P<0.001], 1T:3T [t=6.27, P<0.001], 1T:4T [t=6.04, P<0.001], 1T:5T [t=2.43, P=0.015], 2T:3T [t=2.23, P=0.026], 3T:5T [t=-3.78, P<0.001] and 4T:5T [t=-3.77, P<0.001].For the female population, all ratios were not significantly different except for1T:4T [t=2.56, P=0.011]. However, both feet displayed significant positive association for co-estimation of the corresponding toe length and toe-length ratios (r-values ranging from 0.20-0.70 at P<0.05).Ethnic and sex specific asymmetry were observed in the toe length and toe-length ratios; thus suggesting that anthropometrically, organisms are not completely bilaterally symmetrical.Keywords: Asymmetry, Bilateral symmetry, Toe length, Toe-length ratio, Ethnic groups
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Cui, Yingshun, Sung-Geun Woo, Jangho Lee, Sahastranshu Sinha, Myung-Suk Kang, Long Jin, Kwang Kyu Kim, Joonhong Park, Myungjin Lee, and Sung-Taik Lee. "Nocardioides daeguensis sp. nov., a nitrate-reducing bacterium isolated from activated sludge of an industrial wastewater treatment plant." International Journal of Systematic and Evolutionary Microbiology 63, Pt_10 (October 1, 2013): 3727–32. http://dx.doi.org/10.1099/ijs.0.047043-0.
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A Gram-reaction-positive, rod-shaped, non-spore-forming bacterium (strain 2C1-5T) was isolated from activated sludge of an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, the closest phylogenetic relatives were the type strains of Nocardioides nitrophenolicus (98.6 % similarity), N. kongjuensis (98.5 %), N. caeni (98.4 %), N. simplex (98.3 %), N. aromaticivorans (98.1 %) and N. ginsengisoli (97.5 %); the phylogenetic distance from other species with validly published names within the genus Nocardioides was greater than 3 %. Strain 2C1-5T was characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and iso-C16 : 0, C16 : 0 and C17 : 1ω6c as the major fatty acids. The G+C content of the genomic DNA was 74.9 mol%. These chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain 2C1-5T to the genus Nocardioides . The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 2C1-5T from existing species with validly published names. Therefore, strain 2C1-5T represents a novel species of the genus Nocardioides , for which the name Nocardioides daeguensis sp. nov. is proposed, with the type strain 2C1-5T ( = JCM 17460T = KCTC 19799T).
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Kim, Byung-Chun, Haryoung Poo, Kang Hyun Lee, Mi Na Kim, Doo-Sang Park, Hyun Woo Oh, Jin Man Lee, and Kee-Sun Shin. "Simiduia areninigrae sp. nov., an agarolytic bacterium isolated from sea sand." International Journal of Systematic and Evolutionary Microbiology 62, Pt_4 (April 1, 2012): 906–11. http://dx.doi.org/10.1099/ijs.0.031153-0.
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During a study intended to screen for agar-degrading bacteria, strain M2-5T was isolated from black sand off the shore of Jeju Island, Republic of Korea. Strain M2-5T exhibited agarase activity; the β-agarase gene of the isolate had 62 % amino acid sequence identity to the β-agarase gene of Microbulbifer thermotolerans JAMB A94T. The isolate was closely related to members of the genus Simiduia but was clearly discernible from reported Simiduia species, based on a polyphasic analysis. Cells of strain M2-5T were Gram-negative, catalase- and oxidase-positive, motile rods. The DNA G+C content was 53.3 mol%. The predominant isoprenoid quinone was Q-8. The major cellular fatty acids were C17 : 1ω8c (25.9 %), summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c; 17.2 %) and C17 : 0 (15.0 %). Phylogenetic analysis using 16S rRNA gene sequences showed that strain M2-5T had 96.6 % gene sequence similarity to Simiduia agarivorans SA1T, the most closely related type strain of the genus Simiduia . These results suggest that strain M2-5T represents a novel species in the genus Simiduia , for which the name Simiduia areninigrae sp. nov. is proposed; the type strain is M2-5T ( = KCTC 23293T = NCAIM B 02424T).
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Kim, Kwang Kyu, Keun Chul Lee, and Jung-Sook Lee. "Patulibacter ginsengiterrae sp. nov., isolated from soil of a ginseng field, and an emended description of the genus Patulibacter." International Journal of Systematic and Evolutionary Microbiology 62, Pt_3 (March 1, 2012): 563–68. http://dx.doi.org/10.1099/ijs.0.032052-0.
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A novel actinobacterial strain, designated P4-5T, was isolated from soil of a ginseng field located in Geumsan County, Republic of Korea. Cells of strain P4-5T were Gram-stain-positive, oxidase- and catalase-positive, motile, short rods and the strain produced creamy white colonies on trypticase soy agar. The isolate contained demethylmenaquinone 7 (DMK-7) as the predominant isoprenoid quinone, C18 : 1ω9c and anteiso-C15 : 0 as major fatty acids, diphosphatidylglycerol, phosphatidylglycerol and several unknown lipids in the polar lipid profile, galactose, glucose, mannose, arabinose, xylose (trace) and rhamnose as cell-wall sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content of strain P4-5T was 74.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain P4-5T was related most closely to Patulibacter minatonensis KV-614T and Patulibacter americanus CP177-2T (98.4 and 98.2 % similarity, respectively) and that it formed a separate lineage in the genus Patulibacter. Combined phenotypic and DNA–DNA hybridization data supported the conclusion that strain P4-5T represents a novel species of the genus Patulibacter, for which the name Patulibacter ginsengiterrae sp. nov. is proposed. The type strain is P4-5T ( = KCTC 19427T = CECT 7603T). An emended description of the genus Patulibacter is also provided.
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Liu, Ying, Jing-Hua Jin, Hong-Can Liu, and Zhi-Pei Liu. "Dokdonella immobilis sp. nov., isolated from a batch reactor for the treatment of triphenylmethane dye effluent." International Journal of Systematic and Evolutionary Microbiology 63, Pt_4 (April 1, 2013): 1557–61. http://dx.doi.org/10.1099/ijs.0.042002-0.
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A Gram-staining-negative, non-endospore-forming, non-flagellated, non-motile and rod-shaped bacterium, designated strain LM2-5T, was isolated from activated sludge in a sequencing batch reactor used for the treatment of triphenylmethane dye effluent. The taxonomy of strain LM2-5T was studied by phenotypic, chemotaxonomic and phylogenetic methods. Strain LM2-5T was aerobic, heterotrophic and positive for oxidase but negative for catalase activity. It grew at 16–37 °C (optimum 25 °C) and at pH 5.0–8.5 (optimum between pH 6.5 and pH 7.0). NaCl was not obligatory for growth but was tolerated at concentrations up to 2.0 % (w/v) NaCl. The novel strain formed yellow colonies on trypticase soy agar. Cells of strain LM2-5T were rods that measured 0.3–0.5 µm in width and 3.0–5.0 µm in length. The predominant respiratory quinone was Q-8. The major fatty acids were iso-C15 : 0 and iso-C17 : 1ω9c. The genomic DNA G+C content was 66.7 mol%. In phylogenetic analyses based on 16S rRNA gene sequences, strain LM2-5T clustered with members of the genus Dokdonella and appeared most closely related to Dokdonella koreensis DS-123T (96.4 % sequence similarity), Dokdonella fugitiva A3T (96.1 %), Dokdonella soli KIS28-6T (95.7 %) and Dokdonella ginsengisoli Gsoil 191T (95.7 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain LM2-5T was considered to represent a novel species of the genus Dokdonella , for which the name Dokdonella immobilis is proposed. The type strain is LM2-5T ( = CGMCC 1.7659T = JCM 15763T).
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Chen, Wen-Ming, Che-Chia Yang, Ceshing Sheu, Soon-Wo Kwon, and Shih-Yi Sheu. "Flavobacterium ichthyis sp. nov., isolated from a fish pond." International Journal of Systematic and Evolutionary Microbiology 70, no. 9 (September 1, 2020): 5075–86. http://dx.doi.org/10.1099/ijsem.0.004384.
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Bacterial strain NST-5T, isolated from a fish pond in Taiwan, was characterized using a polyphasic taxonomy approach. Results of phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain NST-5T formed a phylogenetic lineage in the genus Flavobacterium . Analysis of 16S rRNA gene sequences showed that strain NST-5T showed the highest similarity to Flavobacterium enshiense DK69T (94.9 %), Flavobacterium ahnfeltiae 10Alg 130T (94.8 %) and Flavobacterium vireti THG-SM1T (94.8 %). Strain NST-5T showed 68.9–72.5% average nucleotide identity and 19.1–23.7% digital DNA–DNA hybridization identity with the type strains of other close related Flavobacterium species. Cells of the strain were Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped and formed yellow colonies. Optimal growth occurred at 30 °C, pH 7 and with 0.5% NaCl. Strain NST-5T contained iso-C15:0, C15:0 and iso-C16:0 as the predominant fatty acids. The major hydroxyl fatty acids were iso-C16:0 3-OH and iso-C17:0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, three uncharacterized aminophospholipids, two uncharacterized phospholipids and one uncharacterized aminolipid. The major polyamine was homospermidine. The major isoprenoid quinone was MK-6. The DNA G+C content of the genomic DNA was 35.5 mol%. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain NST-5T should be classified as a novel species of the genus Flavobacterium , for which the name Flavobacterium ichthyis sp. nov. is proposed. The type strain is NST-5T (=BCRC 81198T=LMG 31341T).
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Yoon, Jung-Hoon, So-Jung Kang, and Tae-Kwang Oh. "Reclassification of Marinococcus albus Hao et al. 1985 as Salimicrobium album gen. nov., comb. nov. and Bacillus halophilus Ventosa et al. 1990 as Salimicrobium halophilum comb. nov., and description of Salimicrobium luteum sp. nov." International Journal of Systematic and Evolutionary Microbiology 57, no. 10 (October 1, 2007): 2406–11. http://dx.doi.org/10.1099/ijs.0.65003-0.
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A Gram-positive, non-motile, coccoid-shaped, non-spore-forming halophilic bacterial strain, BY-5T, was isolated from a marine solar saltern in Korea and its taxonomic position was investigated by using a polyphasic approach. The novel strain grew optimally at 37 °C and in the presence of 10 % (w/v) NaCl. Strain BY-5T had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-7 as the predominant menaquinone and anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 0 and iso-C17 : 0 as the major fatty acids. The DNA G+C content was 47.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BY-5T formed a coherent cluster with Bacillus halophilus and Marinococcus albus. Strain BY-5T exhibited 16S rRNA gene sequence similarity values of 98.7 and 97.4 % to the type strains of B. halophilus and M. albus, respectively. Strain BY-5T was distinguished from B. halophilus and M. albus by several phenotypic properties and DNA–DNA relatedness data. On the basis of the combined chemotaxonomic and phylogenetic data, it is proposed that M. albus, B. halophilus and strain BY-5T should be placed in a new genus as three separate species. Marinococcus albus and Bacillus halophilus are reclassified in a new genus, Salimicrobium gen. nov., as Salimicrobium album comb. nov. and Salimicrobium halophilum comb. nov., respectively. The type species of the new genus is Salimicrobium album. Strain BY-5T (=KCTC 3989T=CIP 108918T) is placed in the genus Salimicrobium as a novel species Salimicrobium luteum sp. nov.
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Sheu, Shih-Yi, Yang-Shun Lin, and Wen-Ming Chen. "Flavobacterium squillarum sp. nov., isolated from a freshwater shrimp culture pond, and emended descriptions of Flavobacterium haoranii , Flavobacterium cauense , Flavobacterium terrae and Flavobacterium aquatile." International Journal of Systematic and Evolutionary Microbiology 63, Pt_6 (June 1, 2013): 2239–47. http://dx.doi.org/10.1099/ijs.0.046425-0.
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A bacterial strain, designated CMJ-5T, was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain CMJ-5T were Gram-stain-negative, strictly aerobic, non-motile, yellow-pigmented rods surrounded by a thick capsule. Growth occurred at 20–35 °C (optimum, 30 °C), at pH 6.5–8.0 (optimum, pH 7.0) and with 0–0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-5T belonged to the genus Flavobacterium and was related most closely to Flavobacterium haoranii LQY-7T with sequence similarity of 94.4 %. Strain CMJ-5T contained iso-C15 : 0 (37.5 %), iso-C17 : 0 3-OH (13.4 %), iso-C15 : 0 3-OH (13.1 %) and iso-C15 : 1 G (11.2 %) as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. The G+C content of the genomic DNA was 42.4 mol%. On the basis of phylogenetic inference and phenotypic data, strain CMJ-5T should be classified as a representative of a novel species, for which the name Flavobacterium squillarum sp. nov. is proposed. The type strain is CMJ-5T ( = BCRC 80405T = LMG 26890T = KCTC 23915T). Emended descriptions of Flavobacterium haoranii , Flavobacterium cauense , Flavobacterium terrae and Flavobacterium aquatile are also proposed.
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Han, Ji-Hye, Tae-Su Kim, Yochan Joung, Mi Na Kim, Kee-Sun Shin, Taeok Bae, and Seung Bum Kim. "Nocardioides endophyticus sp. nov. and Nocardioides conyzicola sp. nov., isolated from herbaceous plant roots." International Journal of Systematic and Evolutionary Microbiology 63, Pt_12 (December 1, 2013): 4730–34. http://dx.doi.org/10.1099/ijs.0.054619-0.
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Two Gram-stain-positive, non-motile, non-spore-forming, rod-shaped actinobacterial strains were isolated from the surface-sterilized roots of mugwort (Artemisia princeps) and horse-weed (Conyza canadensis), and subjected to taxonomic characterization. 16S rRNA gene sequence analysis indicated that the isolates, designated MWE 3-5T and HWE 2-02T, should be placed in the genus Nocardioides of the family Nocardioidaceae . The strains were closely related to Nocardioides hankookensis DS-30T, which exhibited 16S rRNA gene sequence similarity values of 97.99 and 99.09 % with strains MWE 3-5T and HWE 2-02T, respectively. The genome relatedness of N. hankookensis DS-30T with strain MWE 3-5T was 35.8 %, and that with strain HWE 2-02T was 36.4 %, whereas that between the two isolates was 43.2 %. Strains MWE 3-5T and HWE 2-02T possessed MK-8(H4) as the major isoprenoid quinone, and ll-diaminopimelic acid in the cell-wall peptidoglycan. The main fatty acids were iso-C16 : 0, iso-C17 : 0 and C18 : 1ω9c for strain MWE 3-5T and iso-C16 : 0, 10-methyl C18 : 0 and C18 : 1ω9c for strain HWE 2-02T. Based on phenotypic, genotypic and phylogenetic studies, the following two novel species are proposed: Nocardioides endophyticus sp. nov. (type strain, MWE 3-5T = KCTC 29122T = JCM 18532T) and Nocardioides conyzicola sp. nov. (type strain, HWE 2-02T = KCTC 29121T = JCM 18531T).
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Aslam, Zubair, Ju Hyoung Lim, Wan-Taek Im, Muhammad Yasir, Young Ryun Chung, and Sung-Taik Lee. "Salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood." International Journal of Systematic and Evolutionary Microbiology 57, no. 3 (March 1, 2007): 633–38. http://dx.doi.org/10.1099/ijs.0.64586-0.
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A novel, moderately halophilic, Gram-positive coccus, designated strain S2R53-5T, was isolated from jeotgal, a traditional Korean fermented seafood. The organism was strictly aerobic, non-motile, non-sporulating and catalase- and oxidase-positive. Strain S2R53-5T grew in the presence of 0.5–15 % (w/v) NaCl and at pH 6.5–11.0, with optimum growth at 5 % (w/v) NaCl and pH 7.0. The temperature range for growth was 20.0–30.0 °C, with an optimum temperature of 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S2R53-5T belongs to the family Staphylococcaceae and was most closely related to Salinicoccus roseus DSM 5351T (96.8 % gene sequence similarity), Salinicoccus hispanicus DSM 5352T (96.1 %), Salinicoccus alkaliphilus T8T (95.2 %) and Jeotgalicoccus halotolerans YKJ-101T (95.1 %). The genomic DNA G+C content was 47.0 mol%, which is in the range of 46–51 mol% that is characteristic for the genus Salinicoccus. Levels of DNA–DNA relatedness between strain S2R53-5T and S. roseus DSM 5351T, S. hispanicus DSM 5352T and S. alkaliphilus KCTC 13928T were 32.2, 15.4 and 4.6 %, respectively. Chemotaxonomic data (major menaquinone, MK-6; major fatty acids, iso-C15 : 0 and anteiso-C15 : 0; cell-wall murein type, Lys and Gly) and 16S rRNA gene sequence analysis supported the affiliation of strain S2R53-5T with the genus Salinicoccus. The combined evidence from the low DNA–DNA relatedness, physiological, biochemical and other genotypic data indicate that strain S2R53-5T clearly represents a novel species of the genus Salinicoccus, for which the name Salinicoccus jeotgali sp. nov. is proposed. The type strain is S2R53-5T (=KCTC 13030T=LMG 23640T).
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Chen, Wen-Ming, Shwu-Harn Yang, Chiu-Chung Young, and Shih-Yi Sheu. "Arcicella rigui sp. nov., isolated from water of a wetland, and emended descriptions of the genus Arcicella , Arcicella aquatica , Arcicella rosea and Arcicella aurantiaca." International Journal of Systematic and Evolutionary Microbiology 63, Pt_1 (January 1, 2013): 134–40. http://dx.doi.org/10.1099/ijs.0.037242-0.
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A bacterial strain, designated NSW-5T, was isolated from a water sample taken from Niao-Song Wetland Park in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain NSW-5T were strictly aerobic, Gram-stain-negative, non-motile and polymorphic, being straight, vibrioid, curved and spiral-shaped rods surrounded by a thick capsule and forming light pink-coloured colonies. Some rings consisting of several cells were present. Growth occurred at 10–40 °C (optimum, 25 °C), with 0–3.0 % NaCl (optimum, 0 %) and at pH 6.0–8.0 (optimum, pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NSW-5T belonged to the genus Arcicella with sequence similarities of 98.6, 98.0 and 97.3 % with Arcicella aquatica NO-502T, Arcicella rosea TW5T and Arcicella aurantiaca TNR-18T, respectively. The predominant cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 20.8 %), C16 : 0 (14.6 %), iso-C15 : 0 (13.8 %), C16 : 1ω5c (12.5 %) and C18 : 0 (11.4 %), and the only respiratory quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized glycolipids, aminolipids, phospholipids and aminophospholipids. The DNA G+C content of strain NSW-5T was 44.1 mol%. The DNA–DNA relatedness of strain NSW-5T with respect to recognized species of the genus Arcicella was less than 70 %. On the basis of phylogenetic inference and phenotypic data, strain NSW-5T should be classified as a representative of a novel species, for which the name Arcicella rigui sp. nov. is proposed. The type strain is NSW-5T ( = KCTC 23307T = BCRC 80260T). Emended descriptions of the genus Arcicella and of Arcicella aquatica , Arcicella rosea and Arcicella aurantiaca are also proposed.
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Cirilli, N., R. Munaretto, F. Perticaroli, A. Bruni, N. Baiocco, and B. Fabrizzi. "P005 Lessons from 5T;TG12." Journal of Cystic Fibrosis 19 (June 2020): S56. http://dx.doi.org/10.1016/s1569-1993(20)30342-8.
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Kim, Soo-Jin, Hang-Yeon Weon, Yi-Seul Kim, Rangasamy Anandham, Young-Ah Jeon, Seung-Beom Hong, and Soon-Wo Kwon. "Cohnella yongneupensis sp. nov. and Cohnella ginsengisoli sp. nov., isolated from two different soils." International Journal of Systematic and Evolutionary Microbiology 60, no. 3 (March 1, 2010): 526–30. http://dx.doi.org/10.1099/ijs.0.013581-0.
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Two aerobic, Gram-positive, rod-shaped bacterial strains, 5YN10-14T and GR21-5T, were isolated from the Yongneup wetland and ginseng soil in Korea, respectively. The two strains formed ellipsoidal or oval spores positioned centrally or paracentrally in swollen sporangia. On the basis of 16S rRNA gene sequence analysis, these strains were related to members of the genus Cohnella. 16S rRNA gene sequence similarity between strains 5YN10-14T and GR21-5T was 95.9 %. Strains 5YN10-14T and GR21-5T showed, respectively, 94.3 and 95.2 % 16S rRNA gene sequence similarity to Cohnella thermotolerans CCUG 47242T, 94.6 and 94.4 % to Cohnella hongkongensis HKU3T, 94.7 and 94.7 % to Cohnella laeviribosi RI-39T, and 95.4 and 94.8 % to Cohnella phaseoli GSPC1T. The major fatty acids of strain 5YN10-14T were anteiso-C15 : 0 (51.1 %), iso-C16 : 0 (18.5 %) and C16 : 0 (13.2 %), and the major fatty acids of strain GR21-5T were anteiso-C15 : 0 (48.9 %), iso-C16 : 0 (15.0 %) and iso-C15 : 0 (12.2 %). The two strains contained menaquinone with seven isoprene units (MK-7) as the predominant quinone, and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as major polar lipids; however, strain 5YN10-14T also contained lysylphosphatidylglycerol as a major polar lipid, whereas strain GR21-5T had an unknown aminophospholipid as another major polar lipid. The DNA G+C contents of strains 5YN10-14T and GR21-5T were 58.8 and 61.3 mol%, respectively. Based on the results of the phylogenetic and phenotypic data presented, it was concluded that the two strains represent two novel species of the genus Cohnella, for which the names Cohnella yongneupensis sp. nov. (type strain 5YN10-14T=KACC 11768T=DSM 18998T) and Cohnella ginsengisoli sp. nov. (type strain GR21-5T=KACC 11771T=DSM 18997T) are proposed.
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Kwon, Kae Kyoung, Hee-Soon Lee, Sung Hyun Yang, and Sang-Jin Kim. "Kordiimonas gwangyangensis gen. nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (Kordiimonadales ord. nov.) in the ‘Alphaproteobacteria’." International Journal of Systematic and Evolutionary Microbiology 55, no. 5 (September 1, 2005): 2033–37. http://dx.doi.org/10.1099/ijs.0.63684-0.
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A marine bacterium, designated strain GW14-5T, capable of degrading high-molecular-mass polycyclic aromatic hydrocarbons was isolated from the sediments of Gwangyang Bay, Republic of Korea, after enrichment culture for 2 years with a mixture of benzo[a]pyrene and pyrene. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate forms a phyletic lineage that is distinct from the seven known orders within the ‘Alphaproteobacteria’. 16S rRNA gene sequence similarity of strain GW14-5T to all recognized bacterial species was not greater than 92 %. The dominant fatty acids of the isolate were i-17 : 1 (46·2 %), i-15 : 0 (15·1 %) and i-17 : 0 (12·6 %). The major respiratory quinone was MK-5, and the DNA G+C content was 39·3 mol%. Cells of strain GW14-5T were Gram-negative, motile, catalase-positive, oxidase-positive and weakly halophilic. Glucose, N-acetylglucosamine and maltose were utilized as sole carbon sources. The strain was positive for β-glucosidase activity. Optimal growth of strain GW14-5T was at pH 7·0 and 37–40 °C and required the presence of 2 % (w/v) NaCl. On the basis of this evidence, strain GW14-5T represents a novel genus and species in the ‘Alphaproteobacteria’ for which the name Kordiimonas gwangyangensis gen. nov., sp. nov. is proposed. The novel order Kordiimonadales is proposed for the distinct phyletic line represented by the genus Kordiimonas. The type strain is GW14-5T (=KCCM 42021T=JCM 12864T).
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Zhang, Lei, Ju-Sheng Gao, Shuang Zhang, Rizwan Ali Sheirdil, Xiu-Cheng Wang, and Xiao-Xia Zhang. "Paenibacillus rhizoryzae sp. nov., isolated from rice rhizosphere." International Journal of Systematic and Evolutionary Microbiology 65, Pt_9 (September 1, 2015): 3053–59. http://dx.doi.org/10.1099/ijs.0.000376.
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A Gram-stain-positive, endospore-forming, rod-shaped bacterium, designated 1ZS3-5T, was isolated from rice rhizosphere in Hunan Province, PR China. The isolate was identified as a member of the genus Paenibacillus on the basis of phenotypic characteristics and phylogenetic inference analysis. The 16S rRNA and rpoB gene (β-subunit of bacterial RNA polymerase) sequences were closely related to those of Paenibacillus taihuensis CGMCC 1.10966T with similarities of 97.2 % and 89.7 %, respectively. The DNA–DNA hybridization value between 1ZS3-5T and P. taihuensis CGMCC 1.10966T was 33.4 %. The DNA G+C content of 1ZS3-5T was 47.5 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, unidentified aminophospholipid and unknown phospholipid. The predominant respiratory quinone was MK-7. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0. Based on these results, 1ZS3-5T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus rhizoryzae sp. nov. is proposed. The type strain is 1ZS3-5T ( = ACCC 19782T = DSM 29322T).
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Zhang, Xi-Ying, Ang Liu, Chang Liu, Hai Li, Guo-Wei Li, Zhong Xu, Xiu-Lan Chen, Bai-Cheng Zhou, and Yu-Zhong Zhang. "Arenitalea lutea gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from intertidal sand." International Journal of Systematic and Evolutionary Microbiology 63, Pt_8 (August 1, 2013): 2853–58. http://dx.doi.org/10.1099/ijs.0.049304-0.
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A yellow, rod-shaped, Gram-negative, facultatively aerobic, gliding bacterium, designed strain P7-3-5T, was isolated from intertidal sand of the Yellow Sea, China. Analysis of 16S rRNA gene sequences revealed that strain P7-3-5T formed a distinct lineage within the family Flavobacteriaceae , sharing 94.2–96.9 % sequence similarity with type strains of species of the most closely related genera, including Hyunsoonleella , Jejuia , Marinivirga and Algibacter . The strain grew at 4–40 °C and with 0.5–5.0 % (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed gelatin and DNA. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G and anteiso-C15 : 0 and the major respiratory quinone was MK-6. Polar lipids included phosphatidylethanolamine (PE), three unidentified aminolipids (AL1–3) and four unidentified lipids (L1–4). The genomic DNA G+C content of strain P7-3-5T was 32.1 mol%. Data from this polyphasic study suggest that strain P7-3-5T represents a novel species in a new genus in the family Flavobacteriaceae , for which the name Arenitalea lutea gen. nov., sp. nov. is proposed. The type strain of Arenitalea lutea is P7-3-5T ( = CGMCC 1.12213T = KACC 16457T).
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Yoon, Jung-Hoon, So-Jung Kang, and Tae-Kwang Oh. "Polaribacter dokdonensis sp. nov., isolated from seawater." International Journal of Systematic and Evolutionary Microbiology 56, no. 6 (June 1, 2006): 1251–55. http://dx.doi.org/10.1099/ijs.0.63820-0.
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A Gram-negative, non-motile, non-spore-forming, slightly halophilic bacterial strain, DSW-5T, was isolated from seawater off Dokdo, Korea, and subjected to a polyphasic taxonomic study. It grew optimally at 25–28 °C and in the presence of 2 % (w/v) NaCl. Strain DSW-5T contained MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C15 : 1 and iso-C15 : 0 3-OH as the major fatty acids. The major polar lipids detected were phosphatidylethanolamine, three unidentified phospholipids and an amino-group-containing lipid. The DNA G+C content was 30.0 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DSW-5T was most closely related to the genus Polaribacter. Similarity values between the 16S rRNA gene sequences of strain DSW-5T and the type strains of recognized Polaribacter species were in the range 96.2–96.8 %. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain DSW-5T (=KCTC 12392T=DSM 17204T) was classified in the genus Polaribacter as the type strain of a novel species, for which the name Polaribacter dokdonensis sp. nov. is proposed.
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Wang, Long, Yan Liu, Yanan Wang, Xiaofeng Dai, and Xiao-Hua Zhang. "Celeribacter manganoxidans sp. nov., a manganese-oxidizing bacterium isolated from deep-sea sediment of a polymetallic nodule province." International Journal of Systematic and Evolutionary Microbiology 65, Pt_11 (November 1, 2015): 4180–85. http://dx.doi.org/10.1099/ijsem.0.000558.
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A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped, manganese-oxidizing bacterial strain, designated DY2–5T, was isolated from surface sediment of Pacific Clarion-Clipperton Fracture Zone (CCFZ). Growth occurred at 0–37 °C (optimum 28 °C), pH 6.5–9.0 (optimum pH 7.0–7.5) and in the presence of 1–11 % (w/v) NaCl (optimum 3–4 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel strain was most closely related to Celeribacter halophilus ZXM137T with 96.13 % sequence similarity, and had 16S rRNA gene sequence similarities in the range 93.89–95.87 % with other species of the genus Celeribacter. The dominant fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The polar lipids of strain DY2–5T comprised phosphatidylglycerol, phosphatidylcholine and two unknown aminolipids. The major respiratory quinone was ubiquinone–10 (Q–10). The DNA G+C content of strain DY2–5T was 64.8 mol%. On the basis of the phenotypic, genotypic and physiological evidence, strain DY2–5T represents a novel species of the genus Celeribacter, for which the name Celeribacter manganoxidans sp. nov. is proposed. The type strain is DY2–5T ( = JCM 19384T = KCTC 32473T).
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Xiao, Na, Yongqin Liu, Xiaobo Liu, Zhengquan Gu, Nianzhi Jiao, Hongcan Liu, Yuguang Zhou, and Liang Shen. "Blastomonas aquatica sp. nov., a bacteriochlorophyll-containing bacterium isolated from lake water." International Journal of Systematic and Evolutionary Microbiology 65, Pt_5 (May 1, 2015): 1653–58. http://dx.doi.org/10.1099/ijs.0.000153.
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Yellow or orange-to-brown pigmented, ovoid or rod-shaped, Gram-negative staining, aerobic strains PE 4-5T and N5-10 m-1 were isolated from brackish water in Lake Peng Co and fresh to brackish water in Lake Namtso on the Tibetan Plateau, China. Bacteriochlorophyll a was produced by the isolates. The predominant cellular fatty acids were C16 : 1, C17 : 1 and C18 : 1 unsaturated fatty acids, C17 : 1ω6c (55.3 %), C17 : 1ω8c (13.0 %) and C18 : 1ω7c (10.4 %) for PE 4-5T and C18 : 1ω7c (54.7 %) and C16 : 1ω7c (18.0 %) for N5-10 m-1. The polar lipid profiles of strains PE 4-5T and N5-10 m-1 were composed of diphosphatidylglycerol, phosphatidylcholine (not detected in N5-10 m-1), phosphatidyldimethylethanolamine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, sphingoglycolipid and an unknown phospholipid. The predominant respiratory quinone was ubiquinone Q10 and the DNA G+C content was 66.0 mol% for both strains. The16S rRNA gene sequence of strain PE 4-5T shared 99.0 % similarity with that of N5-10 m-1, and 97.56 % similarity with those of Blastomonas natatoria LMG 17322T and Blastomonas ursincola DSM 9006T, respectively. The DNA–DNA hybridization relatedness between strains PE 4-5T and N5-10 m-1 was 79.0±1.0 %, but below 70 % with the type strains in the genus Blastomonas . Based on the variability of phylogenetic and phenotypic characteristics, the isolates should be classified as representatives of a novel species of the genus Blastomonas ; the name Blastomonas aquatica sp. nov. is proposed. The type strain is PE 4-5T ( = JCM 30179T = CGMCC 1.12851T).
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Lai, Qiliang, Liping Wang, Yuhui Liu, Yuanyuan Fu, Huanzi Zhong, Baojiang Wang, Liang Chen, Jianning Wang, Fengqin Sun, and Zongze Shao. "Alcanivorax pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium." International Journal of Systematic and Evolutionary Microbiology 61, no. 6 (June 1, 2011): 1370–74. http://dx.doi.org/10.1099/ijs.0.022368-0.
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A taxonomic study was carried out on a novel bacterial strain, designated W11-5T, which was isolated from a pyrene-degrading consortium enriched from deep-sea sediment of the Pacific Ocean. The isolate was Gram-reaction-negative and oxidase- and catalase-positive. Growth was observed in 0.5–12 % (w/v) NaCl and at 10–42 °C. On the basis of 16S rRNA gene sequence analysis, strain W11-5T was shown to belong to the genus Alcanivorax with a close relation to A. dieselolei B-5T (93.9 % 16S rRNA sequence similarity), A. balearicus MACL04T (93.1 %), A. hongdengensis A-11-3T (93.1 %), A. borkumensis SK2T (93.0 %), A. venustensis ISO4T (93.0 %) and A. jadensis T9T (92.9 %). Similarities between the gyrB gene sequences of W11-5T and other species of the genus Alcanivorax were between 76.8 and 80.8 %. The principal fatty acids were C12 : 0 3-OH (8.0 %), C16 : 0 (29.1 %) and C18 : 1ω7c (27.4 %). The G+C content of the chromosomal DNA was 60.8 mol%. Based on its morphology, physiology and fatty acid composition as well as the results of 16S rRNA and gyrB gene sequence analyses, strain W11-5T ( = MCCC 1A00474T = CCTCC AB 208236T = LMG 25514T) represents a novel species of the genus Alcanivorax, for which the name Alcanivorax pacificus sp. nov. is proposed.
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Singh, Neha, Melissa M. Kendall, Yitai Liu, and David R. Boone. "Isolation and characterization of methylotrophic methanogens from anoxic marine sediments in Skan Bay, Alaska: description of Methanococcoides alaskense sp. nov., and emended description of Methanosarcina baltica." International Journal of Systematic and Evolutionary Microbiology 55, no. 6 (November 1, 2005): 2531–38. http://dx.doi.org/10.1099/ijs.0.63886-0.
Full textAbstract:
Three novel strains of methylotrophic methanogens were isolated from Skan Bay, Alaska, by using anaerobic cultivation techniques. The water was 65 m deep at the sampling site. Strains AK-4 (=OCM 774), AK-5T (=OCM 775T=DSM 17273T) and AK-9 (=OCM 793) were isolated from the sulfate-reducing zone of the sediments. Each of the strains was a non-motile coccus and occurred singly. Cells grew with trimethylamine as a catabolic substrate and strain AK-4 could also catabolize methanol. Yeast extract and trypticase peptones were not required for growth, but their addition to the culture medium slightly stimulated growth. Each of the strains grew at temperatures of 5–28 °C; they were slight halophiles and grew fastest in the neutral pH range. Analysis of the 16S rRNA gene sequences indicated that strain AK-4 was most closely related to Methanosarcina baltica. DNA–DNA hybridization studies showed 88 % relatedness, suggesting that strain AK-4 represents a novel strain within this species. Strains AK-5T and AK-9 had identical 16S rRNA gene sequences that were most closely related to the sequence of Methanococcoides burtonii (99·8 % sequence similarity). DNA–DNA hybridization studies showed that strains AK-5T and AK-9 are members of the same species (88 % relatedness value), but strain AK-5T had a DNA–DNA relatedness value of only 55 % to Methanococcoides burtonii. This indicates that strains AK-5T and AK-9 should be considered as members of a novel species in the genus Methanococcoides. We propose the name Methanococcoides alaskense sp. nov., with strain AK-5T (=OCM 775T=DSM 17273T) as the type strain.
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Yabe, Shuhei, Yoshifumi Aiba, Yasuteru Sakai, Masaru Hazaka, and Akira Yokota. "Thermogemmatispora onikobensis gen. nov., sp. nov. and Thermogemmatispora foliorum sp. nov., isolated from fallen leaves on geothermal soils, and description of Thermogemmatisporaceae fam. nov. and Thermogemmatisporales ord. nov. within the class Ktedonobacteria." International Journal of Systematic and Evolutionary Microbiology 61, no. 4 (April 1, 2011): 903–10. http://dx.doi.org/10.1099/ijs.0.024877-0.
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Two thermophilic, Gram-stain-positive, sporulating bacterial strains, which formed branched vegetative and aerial mycelia, were isolated from fallen leaves sampled from geothermal soils and designated ONI-1T and ONI-5T. Strain ONI-1T grew at 50–74 °C, with optimum growth at 60–65 °C, and strain ONI-5T grew at 45–74 °C, with optimum growth at 60–65 °C. The pH range for growth of the strains was pH 4.6–8.0, with optimum growth at pH 7.0. The DNA G+C contents of strains ONI-1T and ONI-5T were 60.2 and 58.1 mol%, respectively. The major fatty acid was iso-C17 : 0 and the major menaquinone was MK-9(H2). The cell walls of the strains contained glutamic acid, serine, glycine, histidine, alanine and ornithine. The polar lipids consisted of phosphatidylinositol, phosphatidylglycerol and a glycolipid. The cell-wall sugar was rhamnose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains belong to the class Ktedonobacteria and that strains ONI-1T and ONI-5T are most closely related to Thermosporothrix hazakensis SK20-1T (85.3 and 84.5 % sequence similarity, respectively). 16S rRNA gene sequence similarity between the two strains was 96.6 %. Based on the phenotypic features and phylogenetic position, we propose that strains ONI-1T and ONI-5T constitute a novel genus containing two novel species, for which we propose the names Thermogemmatispora onikobensis gen. nov., sp. nov. (the type species; type strain ONI-1T = JCM 16817T = KCTC 19768T) and Thermogemmatispora foliorum sp. nov. (type strain ONI-5T = JCM 16818T = KCTC 19767T), within the new family Thermogemmatisporaceae fam. nov. and order Thermogemmatisporales ord. nov.
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Kim, Byung-Yong, Hang-Yeon Weon, Sylvie Cousin, Seung-Hee Yoo, Soon-Wo Kwon, Seung-Joo Go, and Erko Stackebrandt. "Flavobacterium daejeonense sp. nov. and Flavobacterium suncheonense sp. nov., isolated from greenhouse soils in Korea." International Journal of Systematic and Evolutionary Microbiology 56, no. 7 (July 1, 2006): 1645–49. http://dx.doi.org/10.1099/ijs.0.64243-0.
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Two yellow-pigmented, Gram-negative, rod-shaped bacterial strains, GH1-10T and GH29-5T, were isolated from greenhouse soils in Korea. 16S rRNA gene sequence analysis indicated that these strains were related to members of the genus Flavobacterium. Strain GH1-10T was most closely related to Flavobacterium psychrolimnae and Flavobacterium denitrificans, with sequence similarities of 95.9 and 95.2 %, respectively. Strain GH29-5T was most closely related to ‘Flavobacterium saliodium’, F. denitrificans and Flavobacterium frigoris, with sequence similarities of 94.3, 92.5 and 92.5 %, respectively. The major cellular fatty acids of GH1-10T were iso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c) and iso-C17 : 0 3-OH, and those of GH29-5T were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 3-OH. Both strains contained menaquinone with six isoprene units (MK-6) as the sole quinone. The DNA G+C contents of GH1-10T and GH29-5T were 35 and 39 mol%, respectively. Based on the phylogenetic and phenotypic data presented, it is concluded that the two bacteria represent two separate novel species of the genus Flavobacterium. The names proposed to accommodate these organisms are Flavobacterium daejeonense sp. nov., with type strain GH1-10T (=KACC 11422T=DSM 17708T), and Flavobacterium suncheonense sp. nov., with type strain GH29-5T (=KACC 11423T=DSM 17707T).
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Wu, Chun-Yuan, Li Zhuang, Shun-Gui Zhou, Fang-Bai Li, and Jian He. "Corynebacterium humireducens sp. nov., an alkaliphilic, humic acid-reducing bacterium isolated from a microbial fuel cell." International Journal of Systematic and Evolutionary Microbiology 61, no. 4 (April 1, 2011): 882–87. http://dx.doi.org/10.1099/ijs.0.020909-0.
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A novel halotolerant, alkaliphilic, humic acid-reducing bacterium, designated MFC-5T, was isolated from a microbial fuel cell that was fed continuously with artificial wastewater (pH 10.0). Cells were Gram-positive-staining, facultatively anaerobic, non-fermentative, non-motile rods and had a G+C content of 59.0 mol%. Microbial growth was observed with <13 % (w/v) NaCl (optimum 10 %), at pH 7.0–11.0 (optimum pH 9.0) and at 25–45 °C (optimum 37 °C). Strain MFC-5T was active in the anaerobic reduction of a humic acid analogue, anthraquinone-2,6-disulphonate, with lactate, formate, acetate, ethanol or sucrose as the electron donor. The major cellular fatty acids were C18 : 1ω9c (42.68 %), C16 : 0 (33.69 %), C18 : 0 (7.56 %), C17 : 1ω8c (5.14 %) and C17 : 0 (3.39 %). Phylogenetic analysis demonstrated that strain MFC-5T displayed >3 % 16S rRNA gene sequence divergence from its closest relatives. Based on phenotypic, genetic and phylogenetic analysis, a novel species, Corynebacterium humireducens sp. nov., is proposed. The type strain is MFC-5T ( = NBRC 106098T = CGMCC 2452T = DSM 45392T).
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Yan, Jian, Jian Hai Yue, and He Yang. "Study on the Fusion of the Train 5T System." Applied Mechanics and Materials 618 (August 2014): 480–83. http://dx.doi.org/10.4028/www.scientific.net/amm.618.480.
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Vehicle operation safety monitoring system (5T system) is the important means to guarantee the safe operation of the vehicle under the new situation of mixed passenger and freight rail transportation, speed, vehicles overloaded. The system using a variety of advanced motion detection technology, information processing technology and network technology to achieve real-time vehicle dynamic monitoring of running status. However, various types of failure of vehicle components are interacting and interrelated, in practical applications, there is a strong demand for information sharing, centralized monitoring and comprehensive analysis. Based on the actual research of Datong-Qinhuangdao line, a set of building methods of 5T information fusion are proposed, laid the foundation for the promotion of 5T information fusion.
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Zhao, Xue Quan, Li Guan, Yi Ping Zhong, Ping Liu, and Wen Ji Deng. "Influence of Morphology of Vacuum-Evaporated Oligothiophene Derivative Films on Oganic Photovoltaic Performance." Solid State Phenomena 181-182 (November 2011): 320–23. http://dx.doi.org/10.4028/www.scientific.net/ssp.181-182.320.
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In order to understand the influence of morphology of vacuum-evaporated conjugated organic thin film on organic photovoltaic performance, oligothiophene derivatives, 2,3,4,5-tetrathienylthiophene (X-5T) and 2,5-dithienyl-3,4-di ((2,2') bithiophene-5yl) thiophene (X-7T), were prepared. The thermal behaviour and morphologies of vacuum-deposited X-5T and X-7T films were examined by differential scanning calorimetry (DSC) and x-ray diffraction (XRD). The influence of morphologies of vacuum-evaporated X-5T and X-7T films on organic photovoltaic performance was hen investigated. It was found that vacuum-evaporated oligothiophene derivative films possessing crystalline morphology could be beneficial to improve organic photovoltaic performance of device by promoting forward interfacial electron transfer and transport.
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Maschio, M., Z. Cannioto, M. Morgutti, and F. Poli. "7 GT repetition on 5T allele." Journal of Cystic Fibrosis 6 (June 2007): S2. http://dx.doi.org/10.1016/s1569-1993(07)60008-3.
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Salaffi, Fausto, Marco Di Carlo, Marina Carotti, and Sonia Farah. "The Patient-Reported Outcomes Thermometer–5-Item Scale (5T-PROs): Validation of a New Tool for the Quick Assessment of Overall Health Status in Painful Rheumatic Diseases." Pain Research and Management 2018 (October 23, 2018): 1–16. http://dx.doi.org/10.1155/2018/3496846.
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Objective. To investigate the construct validity, reliability (internal consistency and retest reliability), and feasibility of the patient-reported outcomes thermometer–5-item scale (5T-PROs), a new tool to measure overall health status in patients with painful chronic rheumatic diseases such as rheumatoid arthritis (RA), psoriatic arthritis (PsA), axial spondyloarthritis (axialSpA), and fibromyalgia (FM). Methods. Consecutive patients have been involved in this study. The following analyses were performed to establish the validity of the 5T-PROs: (1) principal component factor analysis was used to identify the presence of a relatively small number of underlying latent factors than can be used to represent relations among sets of many variables; (2) Cronbach’s alpha was calculated as an indicator of internal consistency; and (3) Pearson product-moment correlations were conducted to assess the convergent validity. The 5T-PROs was also administered a second time (two weeks after the initial administration) to a subset of sample (n = 426) to allow for calculation of test-retest reliability. We used the intraclass correlation coefficient (ICC) as an estimate of test-retest reliability. Additionally, discriminant validity was tested using analysis of variance (ANOVA) with Bonferroni post hoc multiple comparisons, in different disease conditions. Feasibility was analyzed by the time taken in completing the 5T-PROs and the proportion of patients able to complete the 5 item. Results. 1,199 patients (572 with RA, 251 with axialSpA, 150 with PsA, and 226 with FM) were examined. The mean age was 55.7 (standard deviation: 13.1; range: 20 to 80) years. Factor analysis yielded two factors which accounted for 62.54% of the variance of the 5T-PROs. The first factor “Symptom Summary Score” (35.57% of the variance) revealed a good internal consistency (alpha = 0.88); the internal consistency of the second factor “Psychological Summary Score” (26.97% of the variance) was moderate (alpha = 0.69). The reliability of the whole instrument was good (alpha = 0.82). A very high correlation was obtained between Symptom Summary Score and SF-36 PCS and between pain thermometer intensity and SF-36 bodily pain. For all five items and summary scale scores of the SF-36, there was strong evidence that the mean rank of the scores differs significantly between the groups (Kruskal–Wallis tests, p<0.001). Discriminant validity, assessed by comparing the 5T-PRO dimensions in patients with different states of disease activity, showed that the 5T-PROs show moderate association with the presence of comorbidities. It was also noted that it was inversely correlated (p=0.01) to years of formal education. Conclusion. The 5T-PROs is easily administered, reliable and a valid instrument for evaluating the extensive multidimensional impact associated with chronic painful rheumatic conditions.
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Lin, Shih-Yao, Asif Hameed, You-Cheng Liu, Cheng-Zhe Wen, Wei-An Lai, Yi-Han Hsu, and Chiu-Chung Young. "Bacillus lycopersici sp. nov., isolated from a tomato plant (Solanum lycopersicum L.)." International Journal of Systematic and Evolutionary Microbiology 65, Pt_7 (July 1, 2015): 2085–90. http://dx.doi.org/10.1099/ijs.0.000223.
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A Gram-stain-positive, rod-shaped, endospore-forming bacterium (designated strain CC-Bw-5T) was isolated from chopped tomato stems. The isolate grew at 20–40 °C, pH 6.0–8.0 and tolerated 6 % (w/v) NaCl. The most closely related strains in terms of 16S rRNA gene sequence similarity were Bacillus isabeliae (95.3 %) and Bacillus oleronius (95.3 %). The G+C content of the genomic DNA was 37.2 ± 3.6 mol%. Strain CC-Bw-5T was determined to possess C16 : 0, iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 as predominant fatty acids. The polar lipid profile consisted of predominant amounts of diphosphatidylglycerol, and moderate-to-trace amounts of phosphatidylethanolamine, phosphatidylglycerol, two unidentified glycolipids, an unidentified phospholipid and an unidentified phosphoglycolipid. The cell-wall peptidoglycan contained meso-diaminopimelic acid; menaquinone (MK-7) was the predominant respiratory quinone. According to distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-Bw-5T is proposed to represent a novel species within the genus Bacillus for which the name Bacillus lycopersici sp. nov. is proposed. The type strain is CC-Bw-5T ( = BCRC 80623T = JCM 19140T).
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Xu, Hongxiu, Lijing Jiang, Shaoneng Li, Xiang Zeng, and Zongze Shao. "Mameliella atlantica sp. nov., a marine bacterium of the Roseobacter clade isolated from deep-sea sediment of the South Atlantic Ocean." International Journal of Systematic and Evolutionary Microbiology 65, Pt_7 (July 1, 2015): 2255–59. http://dx.doi.org/10.1099/ijs.0.000248.
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A taxonomic study was carried out on strain L6M1-5T, which was isolated from deep-sea sediment collected from the South Atlantic Ocean. The isolate was Gram-reaction-negative, oxidase-negative and catalase-weakly positive. Growth was observed in the presence of 0.5–15 % (w/v) NaCl (optimum 3–5 %), at 10–41 °C (optimum 28–30 °C), and pH 5.0–10.5 (optimum pH 7.0). The principal fatty acids were summed feature8 (C18 : 1ω7c/ω6c) (84.2 %), C18 : 0 (6.3 %), C12 : 1 3-OH (3.2 %) and C16 : 0 (2.7 %). The polar lipid profile comprised phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, two unknown phospholipids and one unknown lipid. Ubiquinone-10 was the major quinone. The G+C content of the genomic DNA was 66.0 mol %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain L6M1-5T belonged to the genus Mameliella and shared 95.8 % sequence similarity with Mameliella alba JLT354-WT. The combined genotypic and phenotypic data show that strain L6M1-5T represents a novel species of the genus Mameliella, for which the name Mameliella. atlantica sp. nov. is proposed. The type strain is L6M1-5T ( = MCCC 1A07531T = JCM 30230T).
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Zhang, Na, Wenfu Wu, Yujia Wang, and Shuyao Li. "Hazard Analysis of Traditional Post-Harvest Operation Methods and the Loss Reduction Effect Based on Five Time (5T) Management: The Case of Rice in Jilin Province, China." Agriculture 11, no. 9 (September 13, 2021): 877. http://dx.doi.org/10.3390/agriculture11090877.
Full textAbstract:
Traditional post-harvest operation methods applied in rice fields lack advanced management knowledge and technology, which has led to post-harvest losses. We proposed the concept of Five Time (5T) management for the first time. 5T management divides the whole life cycle of rice into different growth time interval to complete process management. This paper mainly introduces the management of rice grain period, that is, the post-harvest management period, including the operation process management of harvesting, field stacking, drying, warehousing, and storing. In 2019, our research team formulated the 5T management method, which considers the entire post-harvest process, and carried out a pilot application of this method at the Jilin Rice Industry Alliance of Jilin Province. Moreover, to promote the 5T management method, our research team carried out follow-up experiments in rice production enterprises and found severe post-harvest rice losses. This paper combined a large number of literature and the basic theory research of rice post-harvest to analyze the traditional methods for post-harvest processing and the associated rice losses. By implementing the 5T management method, 4.33% of losses incurred during the T1 harvesting period could be recovered. In the T2 field period, drying rice within 48 h after harvesting could reduce losses by 2.5%. In the T3 drying period, the loss rate could be reduced by 1.6% if traditional drying methods were replaced by mechanical drying and by 0.6% if cyclic drying was implemented to prevent over-drying. In the T5 storage period, the loss rate of 7% could be reduced by adopting advanced grain storage technologies such as low-temperature storage. Overall, the rice loss rate could be reduced by 15.43%, which is equivalent to a yield of 32.68 million tonnes of rice. The important factors in each period are strictly controlled in the 5T management method to prevent the post-harvest losses caused by flawed concepts and improper management and to increase the amount of usable fertile land.
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Elia, Jlenia, Rossella Mazzilli, Michele Delfino, Maria Piane, Cristina Bozzao, Vincenzo Spinosa, Luciana Chessa, and Fernando Mazzilli. "Impact of Cystic Fibrosis Transmembrane Regulator (CFTR) gene mutations on male infertility." Archivio Italiano di Urologia e Andrologia 86, no. 3 (September 30, 2014): 171. http://dx.doi.org/10.4081/aiua.2014.3.171.
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Objective. The aim of this study was to evaluate the prevalence of most common mutations and intron 8 5T (IVS8-5T) polymorphism of CFTR gene in Italian: a) azoospermic males; b) non azoospermic subjects, male partners of infertile couples enrolled in assisted reproductive technology (ART) programs. Material and methods. We studied 242 subjects attending our Andrology Unit (44 azoospermic subjects and 198 non azoospermic subjects, male partners of infertile couples enrolled in ART programs). Semen analysis, molecular analysis for CFTR gene mutations and genomic variant of IVS8-5T polymorphic tract, karyotype and chromosome Y microdeletions, hormonal profile (LH, FSH, Testosterone) and seminal biochemical markers (fructose, citric acid and L-carnitine) were carried out. Results. The prevalence of the common CFTR mutations and/or the IVS8-5T polymorphism was 12.9% (4/31 cases) in secretory azoospermia, while in obstructive azoospermia was 84.6% (11/13 cases; in these, the most frequent mutations were the F508del, R117H and W1282X). Regarding the non azoospermic subjects, the prevalence of the CFTR and/or the IVS8-5T polymorphism was 11.1% (11/99 cases) in severe dyspermia, 8.1% (6/74 cases) in moderate dyspermia and finally 4.0% (1/25 cases) in normospermic subjects. Conclusions. This study confirms the highly significant prevalence of CFTR mutations in males with bilateral absence of the vas deferens or ejaculatory ducts obstruction compared with subjects with secretory azoospermia. Moreover, the significant prevalence of mutations in severely dyspermic subjects may suggest the possible involvement of CFTR even in the spermatogenic process. This could explain the unsatisfactory recovery of sperm from testicular fine needle aspiration in patients affected by genital tract blockage.