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Maninggolang, Alfrida ,., Jeany Sh Polii-Mandang, and Wenny ,. Tilaar. "PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO." AGRI-SOSIOEKONOMI 14, no. 1 (2018): 439. http://dx.doi.org/10.35791/agrsosek.14.1.2018.19730.
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This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.
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., Junairiah, Artifa Rachmah, Yosephine Sri Wulan Manuhara, Ni’matuzahroh ., Lilis Sulistyorini, and Surahmaida . "Pengaruh Hormon Indole Butyric Acid (IBA) dan 6-Benzyl Amino Purin (BAP) terhadap Induksi Kalus Piper betle L. var Nigra." Journal of Pharmacy and Science 4, no. 2 (2019): 85–90. http://dx.doi.org/10.53342/pharmasci.v4i2.141.
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ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh kombinasi konsentrasi zat pengatur tumbuh IBA (Indole Butyric Acid) dan BAP (6-Benzyl Amino Purine) yang paling baik untuk induksi kalus sirih hitam (Piper betle L.). Penelitian ini menggunakan rancangan acak lengkap dengan 25 perlakuan dan setiap perlakuan memiliki 6 ulangan sehingga terdapat 150 unit ekperimen. Pada tahap kultur kalus dilakukan dengan menambahkan zat pengatur tumbuh IBA dan BAP ke dalam medium Murashige and Skoog (MS). Hasil uji tersebut menunjukkan bahwa zat pengatur tumbuh IBA dan BAP dengan kombinasi konsentrasi berbeda berpengaruh terhadap waktu induksi kalus, berat segar dan berat kering kalus sirih hitam. Hasil penelitian menunjukkan waktu tercepat pembentukan kalus pada IBA 2,0 mg/L dan BAP 2,0 mg/L yaitu 10 hari. Berat segar dan berat kering tertinggi pada IBA 2,0 mg/L dan 2,0 mg/L yaitu 0,8507 gram untuk berat segar dan 0,0769 untuk berat kering. Warna kalus adalah putih kehijauan dengan tekstur kompak dan remah.Kata kunci: Induksi kalus, Piper betle L., Indole Butyric Acid, 6-Benzyl Amino Purine.ABSTRACTThe purpose of this research to determine the effect of the combination concentration of growth regulators IBA (Indole Butyric Acid) and BAP (6-Benzyl Amino Purine) was best for callus induction black betel (Piper betle L.). This research used completely randomized design with 25 treatments and 6 replicates of each treatment, hence there were 150 experimental units. At this stage of callus culture was done by adding the growth regulators IBA and BAP into Murashige and Skoog (MS). The test results showed that plant growth regulators IBA and BAP in combination with different concentrations of influence on callus induction time, fresh weight and dry weight callus Piper betle L. The results showed the fastest time of callus formation at IBA 2,0 mg/L and BAP 2,0 mg/L at 10 days. Fresh weight and dry weight of the highest in the IBA 2,0 mg/L and BAP 2,0 mg/L were 0,8507 grams and 0,0769 grams fresh weight to dry weight. The color of callus was white greenish with compact and friable texture.Keywords: Callus induction, Piper betle L., Indole Butyric Acid, 6-Benzyl Amino Purine.
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Karend, Azzah Hanun Abas, Nintya Setiari, Yulita Nurchayati, and Fatimah Azzahra Ahmad Rashid. "Somatic Embryo Enhancing of Phalaenopsis amabilis (L.) Blume Orchid with 6-Benzyl Amino Purine (BAP)." Jurnal Riset Biologi dan Aplikasinya 7, no. 1 (2025): 88–98. https://doi.org/10.26740/jrba.v7n1.p88-98.
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Phalaenopsis amabilis (L.) Blume is an orchid with high economic value because of its beautiful white flowers, yellow labellum, and long stems. Its existence in nature is threatened with extinction, so efforts are needed to cultivate and preserve P. amabilis, one of which is through somatic embryos, which generally require Plant Growth Regulators (PGRs) in the form of 6-Benzyl Amino Purine (BAP). 6-Benzyl Amino Purine (BAP) is used because it is one of the cytokinin hormones proven to induce somatic embryos. This study aims to examine and determine the use of the right BAP concentration to propagate P. amabilis through somatic embryo induction. The explants used were P. amabilis protocorms; because protocorms produced a greater number of somatic embryos than leaf explants, the development of the explants was observed every week for 2 months. This study used a single factor Completely Randomized Design (CRD) in the form of BAP concentrations of 0, 1, 2, 3, and 4 ppm with 20 replications. The results showed that the most optimal treatment for the propagation of P. amabilis somatic embryos was on media with an average number of somatic embryos at a concentration of BAP 3 ppm (65.00) embryo/explain.
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SWARNAM, SWARNABH, and AFAQ MAJID WANI. "STANDARDIZATION OF MICROPROPAGATION TECHNIQUE OF MULBERRY (MORUS ALBA L.)." Asian Journal of Microbiology, Biotechnology & Environmental Sciences 25, no. 04 (2023): 744–47. http://dx.doi.org/10.53550/ajmbes.2023.v25i04.025.
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A standardization of protocol for rapid multiplication of mulberry (Morus alba L.) was established in vitro using nodal explant excised from hydroponically grown mature mulberry plants. Shoot initiation was induced on Murashige and Skoog (MS) medium supplemented with different concentrations of (BAP) 6- Benzyl amino purine. Effect of different concentration of 6- Benzyl amino purine was studied on multiplication of shoot, revealed that elongated shoots (2-3 cm) were cultured on half strength MS medium supplemented with (IBA) indole-3-butyric acid. Higher percentage of shoot initiation (90%) was observed on MS medium supplemented with 2 mg/l 6-bezyl amino purine. Initiated shoots gave a higher average number of shoots and elongation was observed when it was subcultured on MS medium supplemented with 1.4 mg/l 6-bezyl amino purine. A maximum percentage of root formation (70%) was observed when elongated shoots were subcultured on half strength MS medium containing 1.3 mg/l indole-3-butyric acid. Rooted plants transferred to pots containing sterile FYM, garden soil and sand (1:1:1) for acclimatization in green house showed (75%) survival capacity.
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Muchsin, Muchfa Eprilia, Ateng Supriatna, Ayuni Adawiyah, and Adisty Virakawugi Darniwa. "The Effect of Various Concentration BAP (6-Benzyl Amino Purine) on Orchid Growth (Macodes petola (Blume) Lindl.) In-Vitro." BERKALA SAINSTEK 10, no. 1 (2022): 25. http://dx.doi.org/10.19184/bst.v10i1.27091.
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Macodes petola (Blume) Lindl. has an attractive of leaves veins motif with letters pattern, so it has a high economic value. Tissue culture technique is reported as the best way of plant propagation in short time. BAP (6-Benzyl Amino Purine) has a role in stimulating shoot growth with a certain concentration. The purpose of this study was to determine the effect of Various Concentration BAP (6-Benzyl Amino Purine) on Orchid Growth (M petola (Blume) Lindl.) in vitro. This study used a completely randomized design (CRD) with 4 concentration BAP levels (0; 0.5; 1; 1.5 ppm) with 3 replication. Based on the results of the study, it showed that up to 60 DAP (Day After Planting) the composition of the media with a concentration of 0 ppm BAP had a higher average value for the increase in the stem height, number of shoots and number of roots, namely 1.77±0.798 cm, 2.4 ± 1,528 shoot and 2.4 ± 2,082 root. In contrast to the concentration of 1.5 ppm at this concentration it has a low average value the parameters of stem height, number of shoots and number of roots.
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Budisantoso, Iman, Mona Indriani, and Kamsinah Kamsinah. "Effect of BAP (6-Benzyl Amino Purine) Concentration on Growth Micro Cutting of Nepenthes ampullaria." Biosaintifika: Journal of Biology & Biology Education 10, no. 3 (2018): 678–83. http://dx.doi.org/10.15294/biosaintifika.v10i3.15718.
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Conventionally, cultivations of Nepenthes are conducted by using seeds, cutting, and filial separation. However, there are many obstacles come both from time and technical aspect. In vitro culture is an alternative way for cultivating N. ampullaria (Jack,). One of a technique of in vitro culture is micro cutting. BAP (6-benzyl amino purine) growth regulator could be added to optimize the growth of N. ampullaria microcutting. The purpose of this research was to determine the effect of BAP on the growth of Nepenthes microcutting. This research was done experimentally using Completely Randomized Design (CRD). BAP treatment consisted of 5 concentrations: 0; 0.5; 1; 1.5; 2 (ppm), each treatment were multiplied 4 times. The parameters observed were: a time of bud initiation, time of root initiation, total of leaves, total of new buds, total of roots, length of leave, length of root, and height of bud. The data obtained were analyzed with ANOVA (Analisis of Variance) and continued with 5% and 1% LSD (Least Significant Different) test. The result showed that addition of BAP affected the growth of N. ampullaria microcutting in total leaves, length of leave, and total of buds. LSD test proved that 0.57 ppm of BAP was optimal concentration to increase total buds, whit the value reached of 3.86. Here, we found that BAP can be utilized to enhanche N. ampullaria growth on in vitro culture. The benefit of this study is to conserve N. ampullaria in vitro using BAP at 0.57 ppm.
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Khozin, Mohammad Nur, Muhammad Dima Say Mona, Parawita Dewanti, Widya Kristyanti Putri, Sigit Soeparjono, and Didik Pudji Restanto. "Multiplikasi Tunas Tembakau Secara In Vitro Menggunakan Benzyl Amino Purine Dan Furfuryl Amino Purine Melalui Metode Thin Cell Layer." Al-Kauniyah: Jurnal Biologi 18, no. 2 (2025): 349–61. https://doi.org/10.15408/kauniyah.v18i2.40649.
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Tembakau sebagai bahan baku pembuatan rokok mempunyai nilai ekonomi dan ekspor yang tinggi, namun permasalahan perbanyakan secara konvensional sering menghasilkan keturunan yang heterogen dan beberapa komoditas introduksi seringkali mengalami pertumbuhan yang tidak normal pada fase pembibitan sehingga pemenuhan kebutuhan bahan tanam yang seragam sering menjadi kendala. Kultur in vitro dapat menjadi alternatif dalam perbanyakan bahan tanam yang relatif seragam dan tahan terhadap perubahan lingkungan. Penggunaan zat pengatur tumbuh (ZPT) utamanya golongan sitokinin seperti benzyl amino purine (BAP) dan kinetin sangat mendukung pada multiplikasi tunas tembakau. Penelitian ini bertujuan untuk mengetahui konsentrasi BAP dan kinetin yang optimal pada multiplikasi tunas tembakau. Penelitian menggunakan rancangan acak lengkap (RAL) faktorial dengan faktor BAP (0, 2, 3, dan 4 ppm) dan faktor kinetin (0, 3, dan 4 ppm). Hasil penelitian menunjukkan interaksi BAP dan kinetin berpengaruh sangat nyata terhadap kedinian eksplan bertunas, jumlah tunas, dan daun dengan perlakuan terbaik. Konsentrasi BAP 3 ppm + kinetin 4 ppm yang menginduksi tunas pada 8,3 HST; jumlah tunas 81,3; dan jumlah daun 142,3 helai. Penggunaan BAP berpengaruh nyata terhadap kedinian eksplan berkalus dengan perlakuan terbaik pada konsentrasi 3 ppm BAP yang menginduksi kalus pada 10,78 HST. Kinetin berpengaruh sangat nyata terhadap kedinian eksplan bertunas dengan perlakuan terbaik P2M2 yaitu 4 ppm, yang menginduksi tunas pada 8,3 HST. Kesimpulannya perlakuan BAP 3 ppm + 4 ppm kinetin merupakan perlakuan terbaik.
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Jamil, Fauzi Awaludin, Angga Adriana Imansyah, and Melissa Syamsiah. "PENGARUH GA3 DAN BAP TERHADAP PERBANYAKAN TUNAS GERBERA JAMENSONII SECARA IN VITRO." Pro-STek 3, no. 1 (2021): 1. http://dx.doi.org/10.35194/prs.v3i1.1461.
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Tanaman Gerbera (Gerbera Jamensonii) merupakan tanaman hias yang diminati serta memiliki permintaan yang tinggi. Teknologi kultur jaringan merupakan teknologi yang tepat untuk memperoleh hasil percepatan benih yang berkualitas dengan penambahan ZPT (Zat Pengatur Tumbuh). ZPT yang digunakan adalah kombinasi GA (Asam Giberelat) dan BAP (Benzyl Amino Purine). Penelitian ini dilaksanakan pada bulan Juli sampai Agustus 2020, bertempat di Balai Penelitian Tanaman Hias (BALITHI) Segunung. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi GA (Asam Giberelat) dan BAP (Benzyl Amino Purine) terhadap multiplikasi tanaman Gerbera. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) faktorial yang terdiri dari 4 taraf perlakuan dengan masing masing perlakuan terdapat 3 sampel. Parameter penelitian ini meliputi, jumlah tunas, jumlah daun dan panjang daun. Hasil penelitian menunjukan bahwa pemberian G1S1 (GA 3 ppm dan BAP 0,5 ppm) merupakan perlakuan yang paling baik yang mampu menstimulus jumlah tunas dan jumlah daun Gerbera (Gerbera Jamensonii).
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Yanti, Defila, and Mayta Novaliza Isda. "Shoots Induction of nodes (Citrus microcarpa Bunge.) with addition 6- Benzyl Amino Purine (BAP) by In Vitro." Biospecies 14, no. 1 (2021): 53–58. http://dx.doi.org/10.22437/biospecies.v14i1.11192.
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Kasturi orange (Citrus microcarpa Bunge.) is a medicinal plant that contains chemical compounds from secondary metabolites. Limitations of the seed and slow rate of growth is a challenge in the Kasturi orange plant propagation. Propagation of shoots in the kasturi orange can be produced quickly with shoots induction in vitro. This study aims investigate the influence and determine the concentration of BAP in inducing shoots from nodes kasturi orange explants in vitro. This research was conducted at the Integrated Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, The University of Riau from March - September 2020 using completely randomized design (CRD) which consists of 6 (six) treatments than is control BAP (1,2,3,4,5 mg / L). Results showed BAP had a significantly affect the number of shoots and shoot length. The treatment of 1 mg / L BAP produces the highest number of shoots that 2.50. The highest callus growth was in the treatment of 5 mg / L BAP. The shoot appears fastest time is 12.00 day after planting (DAP) contained in the treatment of 2 mg/L BAP.
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Iram, Bashir, A. Kaloo Zahoor, Tun Nisa Chishti Nahida, Rashid Sabreena, and Sayeed Nusrath. "Effect of 6, Benzyl amino purine (BAP) on callus production and shoot regeneration in Bunium persicum B.Fedtsch." International Journal of BioSciences and Technology (IJBST) ISSN: 0974-3987 11, no. 5 (2018): 46–51. https://doi.org/10.5281/zenodo.1489335.
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<strong>Title:</strong> Effect of 6, Benzyl amino purine (BAP) on callus production and shoot regeneration in Bunium persicum B.Fedtsch <strong>Authors & Afffiliation:</strong> Iram Bashir1, Zahoor A. Kaloo2, Nahida Tun Nisa Chishti3, Sabreena Rashid4, Nusrath Sayeed5 1, 2, 4 Plant Tissue Culture Research Laboratory, Department of Botany, University of Kashmir, Hazratbal Srinagar-190006 Jammu & Kashmir, INDIA 3, 5 Government College for Women, M.A Road Srinagar-190001 Jammu & Kashmir, INDIA mir.irambashir@gmail.com, zakallu@yahoo.com, nahidtn@gmail.com, sababhat80@gmail.com, gochi8@gmail.com <strong>ABSTRACT</strong> Various concentrations of BAP were used in order to find out the optimum concentration of this growth substance for callus production as well as for shoot regeneration in Bunium persicum. The optimum concentration of BAP for callus production was BAP 1mg/l, in terms of average number of days taken for callus production (6 days), amount of callus produced and percent culture response (100%). However, BAP at a concentrationof 4 mg/l was found optimum for shoot regeneration. <strong>URL:</strong> http://www.ijbst.org/Home/papers-published/ijbst-2018-volume-11-issue-5
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Harahap, Fauziyah, Kenari Br Sinuraya, Syarifuddin Syarifuddin, et al. "The Effect of IAA and BAP on Root Induction of Cattleya Orchids." JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS 9, no. 2 (2023): 387–97. http://dx.doi.org/10.36987/jpbn.v9i2.4481.
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Orchid is an ornamental plant commodity that has an important meaning in international trade. Until now, orchids are still the center of attention of farmers and lovers of ornamental plants, because of their bright potential as cut flowers and potted plants. The popular and widespread type of orchid is Cattleya sp. The Cattleya orchid is also called the queen of orchids because of its colorful flowers. Cattleya sp. orchids take a long time to propagate by seed, about 4 to 7 years, so other methods are needed to deal with them. Tissue culture is a method for isolating plant parts such as cells, tissues or organs, and cultivating them in an aseptic environment. The purpose of this study was to determine the optimal interaction of growth regulators indole acetic acid (IAA), benzyl amino purine (BAP), and their interactions with the root induction of Cattleya sp. Cytokinins interact with auxin in determining the direction of cell differentiation. In this research method, differences in concentrations of indole acetic acid (IAA: 0, 2, 4, 6 ppm) and concentrations of benzyl amino purine (BAP: 0, 0, 5, 1 ppm) were carried out. Parameters observed were the number of roots and root length. Data were analyzed descriptively and inferentially using ANOVA. The highest number of roots was obtained in the treatment medium containing 0.5 ppm BAP and 6 ppm IAA, which was 6.33. The highest root length was found in the B0I4 treatment (BAP 0 + IAA 4 ppm). The results of the analysis of the interaction variance between the IAA treatment and the BAP treatment were 0.005, meaning that there was a significant effect of the combination of IAA and BAP on the leaf length of Cattleya sp.
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Walangadi, Farhana Rahmatia, Jusna Ahmad, Devi Bunga Pagalla, Novri Youla Kandowangko, and Febriyanti Febriyanti. "Effect of BAP and NAA on Callus Emergence Time of Dumbaya Young Leaf Explants in Vitro." Jurnal Biologi Tropis 25, no. 2 (2025): 1903–11. https://doi.org/10.29303/jbt.v25i2.9045.
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Momordica cochinchinensis, locally known as Dumbaya in Gorontalo Province, is a traditional medicinal plant with underutilized potential due to the extremely hard morphology of its seed coat. This physical barrier limits the penetration of water, air, and nutrients, thereby reducing the success rate of seed germination and plant propagation. The challenge of propagating plants that are difficult to reproduce sexually, such as dumbaya, can be addressed through various approaches, one of which is asexual reproduction using tissue culture techniques. This study aims to address propagation constraints by applying tissue culture techniques supplemented with Plant Growth Regulators (PGRs), specifically BAP (Benzyl Amino Purine) and NAA (Naphthalene Acetic Acid), which are critical factors in successful in vitro plant regeneration. The combination of Benzyl Amino Purine (BAP) and Naphthalene Acetic Acid (NAA) is a commonly used Plant Growth Regulator (PGR) for inducing callus formation and organogenesis, where BAP is effective in stimulating shoot formation, while NAA plays a role in root induction. The research employed an experimental method using a Completely Randomized Design (CRD) with five treatment levels: H0: MS + 0 ppm NAA + 0 ppm BAP; H1: MS + 1.5 ppm NAA + 0.5 ppm BAP; H2: MS + 3.0 ppm NAA + 0.5 ppm BAP; H3: MS + 1.5 ppm NAA + 1.0 ppm BAP; and H4: MS + 3.0 ppm NAA + 1.0 ppm BAP. Each treatment was replicated three times. The results showed that treatment H1 produced the earliest callus formation, with an average callus initiation time of 7 days after planting.
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Nabila, Cut Tasya, Marai Rahmawati, and Elly Kesumawati. "Pengaruh Konsentrasi 2,4-Dichlorophenoxyacetic Acid dan Benzyl Amino Purin terhadap Induksi Tunas Tanaman Nilam (Pogostemon cablin Benth.) Varietas Tapak Tuan secara In Vitro." Jurnal Ilmiah Mahasiswa Pertanian 7, no. 4 (2022): 193–200. http://dx.doi.org/10.17969/jimfp.v7i4.22394.
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Pengaruh Konsentrasi 2,4-Dichlorophenoxyacetic Acid dan Benzyl Amino Purin terhadap Induksi Tunas Tanaman Nilam (Pogostemon cablin Benth.) Varietas Tapak Tuan secara In Vitro Abstrak. Nilam merupakan tumbuhan penghasil minyak atsiri potensial yang menyuplai 90 % kebutuhan minyak nilam dunia. Faktor-faktor yang menyebabkan rendahnya produktivitas nilam antara lain: mutu genetik tanaman, kurang tepatnya budidaya, pengendalian penyakit, pengelolaan panen dan pascapanen. Produksi nilam dapat ditingkatkan dengan teknik propagasi secara in vitro. Eksplan yang digunakan adalah tunas nilam varietas Tapak Tuan. Penelitian bertujuan untuk memperoleh konsentrasi 2,4-Dichlorophenoxyacetic acid (2,4-D) dan Benzyl Amino Purin (BAP) yang optimal untuk induksi tunas nilam (Pogostemon cablin Benth.) varietas Tapak Tuan secara in vitro yang dilaksanakan pada bulan Desember 2021-Mei 2022 di Laboratorium Kultur Jaringan Tanaman, Fakultas Pertanian, Universitas Syiah Kuala, Banda Aceh. Parameter yang diteliti adalah persentase hidup eksplan, persentase tumbuh tunas adventif, dan persentase muncul kalus. Hasil penelitian menunjukkan bahwa persentase hidup eksplan dan persentase muncul kalus terbaik sebesar 100 % terdapat pada 0,5 mg L-1 2,4-D + 0,5 mg L-1 BAP dan 1,5 mg L-1 2,4-D +1,5 mg L-1 BAP. Persentase tumbuh tunas adventif terbaik sebesar 66,67 % dijumpai pada 1 mg L-1 BAP. The Effect of 2,4-Dichlorophenoxyacetic acid and Benzyl Amino Purin Concentration on Shoot Induction of Patchouli (Pogostemon cablin Benth.) variety of Tapak Tuan In Vitro Abstract. Patchouli is a potential essential oil-producing plant that supplies 90% of the world's patchouli oil needs. Factors that cause low patchouli productivity include: genetic quality of the plant, less precise cultivation, disease control, harvest and post-harvest management. Patchouli production can be increased by in vitro propagation techniques. The explants used were patchouli shoots of the Tapak Tuan variety. Research aimed to obtain optimal concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and Benzyl Amino Purine (BAP) for the induction of patchouli shoots (Pogostemon cablin Benth.) of the Tapak Tuan variety in vitro, conducted at December-May 2022 on Plant Tissue Culture Laboratory, Faculty of Agriculture, Syiah Kuala University, Banda Aceh. The parameters studied were the percentage of explant survival, the percentage of adventitious shoot growth, and the percentage of callus emergence. The results showed that the percentage of surviving explants and the percentage of the best callus appearing 100% were found at 0.5 mg L-1 2,4-D + 0.5 mg L-1 BAP and 1.5 mg L-1 2.4 -D +1.5 mg L-1 BAP. The best growth percentage of adventitious shoots was 66.67% found at 1 mg L-1 BAP.
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Ulinnuha, Zulfa. "Respons Pertumbuhan Anggrek Cymbidium ensifolium terhadap Aplikasi Pupuk Amino-Age dan Benzyl Amino Purine (BAP)." AGROSCRIPT: Journal of Applied Agricultural Sciences 6, no. 2 (2024): 184–92. https://doi.org/10.36423/agroscript.v6i2.2055.
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Anggrek Cymbidium adalah salah satu jenis tanaman hias yang bernilai ekonomi tinggi, sehingga penelitian peningkatan pertumbuhan anggrek Cymbidium perlu dilakukan. Penelitian ini bertujuan untuk mengetahui respon anggrek Cymbidium terhadap berbagai konsentrasi pupuk Amino-Age dan BAP. Penelitian dilakukan di screenhouse Universitas Jenderal Soedirman Purwokerto pada periode Februari hingga Mei 2024. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian pupuk cair Amino-Age yang mengandung asam amino dan sitokinin (BAP) terhadap pertumbuhan vegetatif serta perbanyakan tanaman anggrek Cymbidium. Penelitian ini menggunakan Rancangan Acak Kelompok Lengkap (RAKL) dengan tiga kali ulangan. Perlakuan yang diberikan terdiri dari empat tingkat konsentrasi pupuk Amino-Age (0 mL L-1, 1 mL L-1, 2 mL L-1, dan 3 mL L -1) serta tiga tingkat konsentrasi sitokinin (0 ppm, 150 ppm, dan 300 ppm). Variabel yang diamati meliputi waktu munculnya tunas baru, waktu munculnya daun baru, jumlah anakan, tinggi anakan, dan jumlah daun baru. Hasil penelitian menunjukkan bahwa asam amino mempengaruhi waktu munculnya tunas baru, waktu munculnya daun baru, dan tinggi anakan, sementara sitokinin (BAP) dan interaksi antara asam amino dan sitokinin (BAP) tidak berpengaruh terhadap semua variabel yang diamati. Perlakuan dengan pupuk Amino-Age pada konsentrasi 1 mL L-1 memberikan hasil terbaik untuk waktu muncul tunas dan daun baru, sementara konsentrasi 2 mL L-1 memberikan hasil terbaik untuk tinggi tanaman.
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Suharjo, Usman Kris Joko, Hasanudin Hasanudin, Tunjung Pamekas, Hesti Pujiwati, and Alyi Vanturini. "Promoting Tuber Formation In Vitro with Benzyl Amino Purine and Paclobutrazol at Different Concentrations." Akta Agrosia 22, no. 1 (2019): 29–35. http://dx.doi.org/10.31186/aa.22.1.29-35.
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A common problem related to the production of potatoes in Indonesia is the use of poor quality seed sources and declining seeds. Plant tissue culture is one of the best methods used for the provision of quality seeds. This study aims to establish the best combination of BAP concentration and the concentration of paclobutrazol in spurring the growth of cuttings and initiation of potato tubers in vitro.This research was conducted in November 2017 until June 2018 at Agronomy Laboratory of Biotechnology Division and Plant Culture Network of Agriculture Faculty of Bengkulu University. The research design used was Completely Randomized Design (CRD) which consist of 18 combination of BAP treatment and Paclobutrazol was repeated 6 times. The concentration of BAP used was 0 mg / l, 5 mg / l, 10 mg / l and the concentration of Paclobutrazol 0 mg / l; 2.5 mg / l; 5 mg / l; 7.5 mg / l; 10 mg / l; 12.5 mg / l. The data of the research were analyzed by F test of 5% level and if there were different between treatments followed by Duncan's Multiple Range Test (DMRT) test of5% level. The results showed that the combination of BAP and Paclobutrazol concentration had significant effect on all observed variables. Treatment of 5 mg / l BAP and 7.5 mg / l Paclobutrazol is the best medium in inducing potato micro tube as indicated by the fastest tuber formation time, highest percentage of productive crops, largest number of tubers per bottle, number of tubers per productive plant, tuber diameter the largest and highest wet weight of tuber per bottle. Keywords : in vitro, BAP, paclobutrazol, Potato
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Syahirah, Arifah, Marai Rahmawati, and Elly Kesumawati. "Pengaruh Konsentrasi ZPT BAP dan NAA terhadap Pisang Barangan Merah (Musa acuminata Colla) secara Kultur Jaringan." Jurnal Ilmiah Mahasiswa Pertanian 7, no. 4 (2022): 178–84. http://dx.doi.org/10.17969/jimfp.v7i4.22392.
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Abstrak. Tanaman pisang barangan merah merupakan salah satu pisang lokal yang memiliki pangsa pasar yang besar. Keuntungan perbanyakan dengan melalui teknik kultur jaringan antara lain diperoleh dari bahan tanaman yang unggul dalam jumlah banyak dan seragam serta bebas penyakit. Eksplan yang digunakan dalam penelitian ini adalah bonggol pisang. Zat Pengatur Tumbuh yang digunakan yaitu Benzyl Amino Purine (BAP) dan Naphthalene Acetic Acid (NAA). Tujuan dilakukannya penelitian ini untuk mengetahui pengaruh pemberian zat pengatur tumbuh BAP dan NAA terhadap pembentukan planlet pisang barangan merah serta interaksi kedua faktor tersebut. Penelitian ini menggunakan 2 faktor dengan konsentrasi ZPT BAP yaitu : Kontrol, 1, 3, 5 dan ZPT NAA yaitu : Kontrol, 2, 4, 6 mg L-1. Terdapat 16 perlakuan, masing-masing perlakuan diulang 3 kali dengan total 48 botol eksplan. Hasil penelitian menunjukkan persentase eksplan hidup paling tinggi yaitu 100%, Jumlah tunas pada konsentrasi 3 mg L-1 BAP + 2 mg L-1 NAA memiliki jumlah tunas paling banyak yaitu 4,6. Jumlah daun paling banyak yaitu 1,3 terdapat pada konsentrasi 3 mg L-1BAP + 4 mg L-1 NAA.Kata Kunci: BAP, Konsentrasi, NAA, Pisang Barangan Merah, dan Planlet The Effect of Concentration of PGR BAP and NAA on Barangan Merah Banana (Musa acuminata Colla) by Tissue CultureAbstract. The Barangan Merah banana plant is one of the local bananas that has a large market share. The advantages of procuring seeds through tissue culture are obtained from superior plant material in large and uniform quantities and free of disease. The explant used in this study was banana weevil. he growth regulators used are Benzyl Amino Purine (BAP) and Naphthalene Acetic Acid (NAA). The purpose of this study was to determine the effect of BAP and NAA growth regulators on the formation of Barangan Merah bananas plantlets and the interaction of these two factors. This study used 2 factorial with BAP ZPT concentrations, namely: 0, 1, 3, 5 and ZPT NAA, namely: 0, 2, 4, 6 mgL-1. There were 16 treatments, each treatment was repeated 3 times for a total of 48 bottles of explants. The results showed that the highest percentage of live explants was 100%. The number of shoots on 3 mg L-1 BAP + 2 mg L-1 NAA concentrate had the highest number of shoots, namely 4.6. The highest number of leaves, 1.3, was found in 3 mg L-1 BAP + 4 mg L-1 NAA concentrate.Keywords : BAP, Concentrate, NAA, Barangan merah banana, and Planlet.
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Ibrahim, M. S. D., I. Sulistiyorini, and C. Tresniawati. "Effect of 6-benzyl amino purine on the multiplication ability of shoots of various sizes of porang (Amorphophallus muelleri Blume) bulbils." IOP Conference Series: Earth and Environmental Science 974, no. 1 (2022): 012091. http://dx.doi.org/10.1088/1755-1315/974/1/012091.
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Abstract Porang plant is very prospective and developed because it has high economic value. The high demand for exports has resulted in increased needs for seeds. Tissue culture using plant regulator 6-Benzyl Amino Purine (BAP) has been widely used for seed propagation. This study aimed to examine the effect of adding BAP on the multiplication of shoots at different bulbil weights. Bulbils (corm leaf) were used as explants and cultured on Murashige and Skoog medium containing BAP (according to treatment), 30 g L−1 sucrose, and 2.5 g L−1 phytagel. This study used two factors Completely Randomized Design with five replications. The first factor was concentrations of BAP i.e., 0.0, 1.0, 2.0 and 3.0 mg L−1 and second factor was bulbil weight i.e., 0.25, 0.50 and 1.00 g. There was significant interaction at the number of buds and shoots, but not for other parameters. The results showed that bulbil weight had a significant effect on the number of buds, shoots, and plantlet height, while the concentration of BAP had a significant effect on all parameters. Bulbil size < 1 gram can reproduce seeds with the highest multiplication ability when 3 mg L−1 BAP was given.
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Erawati, Dyah Nuning, Yusriatul Mawaddah, Siti Humaida, and Irma Wardati. "Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)." Jurnal Ilmiah Inovasi 21, no. 1 (2021): 54–57. http://dx.doi.org/10.25047/jii.v21i1.2636.
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Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.
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Saputri, Mulia, Marai Rahmawati, and Elly Kesumawati. "Pertumbuhan Tunas Pisang Barangan (Musa Acuminata Colla.) akibat Pemberian Benzyl Amino Purin dan Arang Aktif secara In Vitro." Jurnal Ilmiah Mahasiswa Pertanian 4, no. 1 (2019): 73–90. http://dx.doi.org/10.17969/jimfp.v4i1.10242.
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Pertumbuhan Tunas Pisang Barangan Akibat Pemberian Benzyl AminoPurin dan Arang Aktif secara In Vitro Accretion of Barangan Banana Shoot Effect of (BAP) and Activated Charcoal Explant by In VitroAbstrak. Banana as a superior product of horticultural crops, has not achieved high productivity and has several obstacles in its multiplication. Tissue culture is one solution to overcome this problem. This study aims to determine the composition of PGRBenzil Amino Purine (BAP) and activated charcoal that are appropriate in the multiplication of barangan banana shoots. This research was conducted at the Tissue Culture Laboratory Faculty of Agriculture Syiah Kuala University, Darussalam Banda Aceh. The design used was a Completely Randomized Design (CRD) with two treatment factors. The first factor is BAP concentration consisting of 3 levels, namely 4 mg/L, 6 mg /L, and 8 mg/L. The second factor is activated charcoal concentration consisting of 3 levels, namely control, 1 g/L and 2 g/L. In this study, from 9 treatment combinations, only 4 treatment combinations were not contaminated. Of the 4 treatment combinations the combination of BAP concentration of 6 mg/L and control (without activated charcoal) showed the fastest shoot growth time of 29 days after multiplication, the most shoot growth was 6 shoots and the average shoot height was 15.9 mm
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Saepudin, Adam, Amir Amilin, Undang Undang, and Tini Sudartini. "Kultur In Vitro Pisang Cavendish (Musa acuminata L.) Pada Media Dengan Konsentrasi Berbeda Ekstrak Jambu Batu Dan Benzyl Amino Purine." Paspalum: Jurnal Ilmiah Pertanian 11, no. 1 (2023): 87. http://dx.doi.org/10.35138/paspalum.v11i1.481.
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In vitro culture requires suitable media, the addition of BAP as a cytokinin is considered to accelerate the growth of Cavendish bananas. Constraints of browning in explants are also often found in the growth of banana explants. The addition of guava extract as an organic antioxidant and BAP is expected to suppress browning and improve the growth of Cavendish banana explants in vitro. The study was aimed to obtain the best concentration of guava extract (Psidium guajava) and Benzyl Amino Purine for the growth of Cavendish banana (Musa acuminata) explants in vitro. This study used a completely randomized design (CRD) with a factorial pattern of 4 x 3 with 4 replications. Treatment of guava extract added to the media (0 g/l, 1 g/l, 2 g/l, 3 g/l) and BAP (3 ppm, 6 ppm, 9 ppm). The data were analyzed using variance with the F test and continued with Duncan's Multiple Distance Test with 5% significance level. The results showed that the best result for the number of shoots were in the treatment of 1 g/l guava extract + BAP 3 ppm. In the number of leaves and high shoots the best result were in the treatment of 0 g/l guava extract + BAP 6 ppm
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Kone, Dramane, Oi Kouadio Samuel Kouadio, Oumar Silue, Affoué Rachelle N’Guessan, Navigué Yeo, and Tanoh Hilaire Kouakou. "Optimization of bud disinfection technique and influence of growth regulators on micropropagation in ginger (Zingiber officinale Rosc.)." International Journal of Biological and Chemical Sciences 16, no. 6 (2023): 2892–904. http://dx.doi.org/10.4314/ijbcs.v16i6.33.
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Ginger (Zingiber officinale Rosc.) is a spice, considered as a food medicine because of its numerous beneficial actions on health. However, its production faces several constraints, including the lack of efficient planting material. This is a limiting factor for industrial ginger production. The present study therefore aimed at developing an effective in vitro regeneration protocol for ginger. First, three disinfecting agents (sodium hypochlorite, calcium hypochlorite and mercury chloride) were tested. Then, different combinations of naphthalene acetic acid (0.5 mg/L NAA) and/or benzyl amino purine (1, 3 and 5 mg/L BAP) were evaluated on in vitro shoots regeneration. The results revealed that 3.6% sodium hypochlorite, used for 20 min, induced the best disinfection rates (100%) and healthy buds (84.66%). Furthermore, this study showed that the tested hormonal combinations significantly influenced shoots proliferation in ginger. However, the Proliferation Medium 4 (PM4) [Murashige and Skoog medium including vitamin B5 (MSB) + 0.5 mg/L of Naphthalene Acetic Acid (ANA) + 5 mg/L of Benzyl Amino Purine (BAP)] was the most effective. It induced the highest average number of shoots (22.83 shoots) with an induction rate of 80.50%. As a result of this study, 3.6% sodium hypochlorite used for 20 min and MP4 medium (MSB + 0.5 mg/L ANA + 5 mg/L BAP) were selected for in vitro ginger regeneration.
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Pyati, Ashok N. "In vitro Regeneration of an Endangered Medicinal Orchid Dendrobium crepidatum Lindl. & Paxton through Protocorm like bodies." Plant Tissue Culture and Biotechnology 33, no. 1 (2023): 1–8. http://dx.doi.org/10.3329/ptcb.v33i1.66753.
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A protocol for regeneration of an endangered medicinal orchid Dendrobium crepidatum was established mainly via indirect protocorm like body (PLBs) formation. The PLBs procured from in vitro growth cultures was assessed in ½ MS medium alone and in combinations with 6-Benzyl amino purine (BAP), Kinetin (Kn) and α-Naphthalene acetic acid (NAA). The calli were induced from longitudinally bisected segments of PLBs. The optimum percentage of callus was obtained in ½ MS medium supplemented with BAP (1.0 mg/l) and combination of BAP (1.0 mg/l) with NAA (0.5 mg/l), where 64.3% and 83.3% of callus was formed respectively. PLB formation was achieved when callus was transferred onto the respective combinations in ½ MS basal medium. The optimum percentage (91.6%) of PLBs were regenerated in the combination of BAP with NAA. These PLBs differentiated into intact plantlets about 3 cm in height and with 4-5 roots were transplanted into potting mixture with highest rate of 93.3% survival rate in a green house.
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Mataa, M., C. Musenga, and C. Hakachite. "Shelf life responses of ‘Akito’ rose (Rosa spp.) cut flowers treated with growth regulator benzyl amino purine and microbiocide aluminium sulphate." International Journal of Agricultural Research, Innovation and Technology 10, no. 1 (2020): 6–12. http://dx.doi.org/10.3329/ijarit.v10i1.48088.
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Rose (Rosa spp.) production for cut flowers is an integral part of the horticultural industry. However, because roses are exotic plants they exhibit serious problems related to poor climatic adaptation. A study was conducted at the School of Agricultural Sciences, University of Zambia to evaluate chemicals to extend flower shelf life. The cultivar ‘Akito’ was used as the test variety. Benzyl amino purine ([6- (benzylamino) purine]) (BAP), a growth regulator and aluminium sulphate (an acidifying reagent) were applied to cut flowers at 0, 5, 10, 15, and 20 mg L-l and 0, 400, 800 and 1200 mg L-l, concentration, respectively. BAP was applied to the plants in the greenhouse 2 days before harvest. A randomised complete block design arranged as a two- factor- factorial arrangement with three replications was used. Harvesting was done at the loose open calyx stage. Aluminium sulphate was applied to the holding solution where flowers were kept after harvest. The pH of the holding solution, blossoming (flower opening), leaf color of subtending leaves and bacterial population were monitored. The 400 mg L-l aluminium sulphate treatment caused an increase in acidity. There was concurrent decline in bacterial count in the first three days of application at less than 1 x 106 which was five times lower than the water control treatment. Bacterial population followed the trend of water acidity. The 20 mg L-l BAP application increased shelf life by 35 %. The results showed that BAP and aluminum sulphate treatment could enhance shelf life.
 Int. J. Agril. Res. Innov. Tech. 10(1): 6-12, June 2020
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Thorat, M., and Rupali Taur. "Micropropagation of Pomegranate (Punica granatum L.) ‘Ganesh’ cultivar from nodal explants." Plantae Scientia 7, no. 1 (2024): 16–21. http://dx.doi.org/10.32439/ps.v7i1.16-21.
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The present study was carried out to develop a rapid in vitro multiplication protocol using nodal explants. Complete plantlets have been successfully regenerated from node explants. The explants were incubated on Murashige and Skoog (MS) culture medium supplemented with different combinations of 6-benzyl amino purine (BAP) Indole acetic acid (IAA) and kinetin. Multiplication of node explants was achieved on BAP while from other combinations of growth hormone,. The response of various growth regulators was investigated. The combination of showed the highest rate of shoot induction (100%) and a shoot regenerated about 6 shoots per explants when cultured on MS media supplemented with BAP (4 µM) within 2 weeks. The multiple elongated shoots were obtained on MS basal medium combination of Activated charcoal and growth hormones. Well-developed roots were achieved on MS medium in combination with BAP and IAA as well as 80% of plantlets were survived in the soil successfully in the hardening process. A robust and optimized protocol will be helpful for the mass production of this economical important fruit .
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Vaidya, Kanchan, and Y. Bansal. "Multiple Shoot Induction from Apical Buds of mature Semul (Bombax ceiba L.)." Indian Journal of Forestry 27, no. 2 (2004): 175–78. http://dx.doi.org/10.54207/bsmps1000-2004-tbliy2.
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Multiple shoots (8-45) were obtained from apical bud explants of mature B. ceiba L. on woody plant medium supplemented with Naphthalene acetic acid (2.68 mM) in combination with Benzyl amino purine (22.19 mM). Shoots were elongated on WPM augmented with BAP (0.44 mM) and Adenine sulphate (5 mg/l). In vitro multiplied shoots could be rooted on medium containing Indole butyric acid (2.46 mM), BAP (0.44 mM) and 2% sucrose. Plantlets were hardened and transferred to pots containing sand and soil (1:1), acclimatized and have been established in field.
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Shakira, Afnan Mohammad Sadar1 Sowmiya Madhaiyan1 Sneaha Mahadevan1 Shalini Govindaraj1 Sindhuja Ravichandran1 Arul Ganesh Thangaraj2 Valarmathi Muthu2 sarankumar Chandran3 *. "Micropropagation (Shoot initiation and Development) of Hibiscus from nodal explants using BAP (6-Benzyl Amino Purine)." Trends in Agriculture Science 1, no. 8 (2022): 353–57. https://doi.org/10.5281/zenodo.7239822.
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In this study, the nodal explants collected from the healthy shoots were cultured on the basal MS medium supplemented with 3mg/lit of BAP (6-Benzyl Amino Purine). In the earlier study, several researchers reported that the concentration of 3mg/lit is optimum for the growth and development of shoot tip in Hibiscus by the duration of 1month. The different stage of shoot development was observed from the third day to one month interval. It revealed that the callus initiation starts from the 10<sup>th</sup> day and auxiliary shoot initiation from 2 weeks, and subsequent growth and development was observed from the third week and one month interval respectively. Further the developed healthy shoot culture will be maintained and will be treated with NAA at different concentration for root initiation. Finally, the optimum concentration of NAA will be fixed for better root initiation and development
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Rosliani, Rini, ER Palupi, and Yusdar Hilman. "Penggunaan Benzil Amino Purin dan Boron untuk Meningkatkan Produksi dan Mutu Benih True Shallots Seed Bawang Merah (Allium cepa var. ascalonicum) di Dataran Tinggi." Jurnal Hortikultura 22, no. 3 (2013): 242. http://dx.doi.org/10.21082/jhort.v22n3.2012.p242-250.
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ABSTRAK. Penggunaan umbi benih untuk bahan perbanyakan bawang merah mempunyai beberapa masalah, antara lain ketidaktersediaan benih bermutu, produktivitas rendah, dan mahal. Salah satu alternatif teknologi yang potensial untuk dikembangkan sebagai benih ialah penggunaan biji botani atau true shallots seed (TSS). Penelitian dilakukan untuk memproduksi benih bawang merah TSS di dataran tinggi melalui peningkatan pembungaan dan viabilitas serbuk sari menggunakan BAP dan boron. Penelitian dilakukan di Kebun Percobaan Balai Penelitian Tanaman Sayuran Lembang, Jawa Barat (ketinggian 1.250 m dpl.) dari Bulan Agustus 2011 sampai dengan Februari 2012. Rancangan percobaan yang digunakan yaitu acak kelompok faktorial dengan tiga ulangan. Perlakuan terdiri atas dua faktor, yaitu aplikasi benzil amino purin (BAP) 0, 50, 100, 150, dan 200 ppm dan boron 0, 1, 2, 3, dan 4 kg/ha. Aplikasi BAP diberikan tiga kali pada umur 1, 3, dan 5 minggu setelah tanam (MST), dan boron pada umur 3, 5, dan 7 MST. Hasil penelitian menunjukkan bahwa aplikasi BAP dapat meningkatkan pembungaan, viabilitas serbuk sari bawang merah, dan produksi benih TSS tetapi tidak meningkatkan mutu benih, sedangkan aplikasi boron efektif meningkatkan semua variabel yang diamati termasuk mutu benih TSS. Konsentrasi BAP yang optimum untuk menghasilkan produksi benih TSS ialah 37,5 ppm, sedangkan dosis boron yang optimum untuk menghasilkan bobot benih per plot yang tinggi dengan mutu benih sesuai standar sertifikasi mutu yaitu 2,88 kg/ha. Hasil yang diperoleh pada perlakuan boron memberikan peningkatan sebesar 165,69% daripada kontrol. Hasil penelitian ini memberikan informasi teknologi produksi TSS yang dapat dikembangkan untuk memproduksi benih TSS bermutu tinggi. <br /><br />ABSTRACT. Rosliani, R, Palupi, ER, and Hilman, Y 2012. Benzyl Amino Purine and Boron Application for Improving Production and Quality of True Shallots Seed (Allium cepa var. ascalonicum) in Highlands. The use of bulb for propagation material of shallots has several problems including unavailability of quality seeds, low productivity, and expensive. One of the potential alternative technologies to be developed as seed is using true shallots seed (TSS). The aimed of research was to produce TSS in the highlands through increased flowering and pollen viability by using benzyl amino purine (BAP) and boron. The study was conducted at the Experimental Field, Indonesian Vegetable Research Institute (IVEGRI) in Lembang, West Java (altitude 1,250 m asl.), from August 2011 to February 2012. The factorial experiment was arranged in a randomized block design with three replications. The treatments consists of two factors, namely the application of BAP 0, 50, 100, 150, and 200 ppm and boron (0, 1, 2, 3, and 4 kg/ha). Benzyl amino purine application was given three times at 1, 3, and 5 weeks after planting (WAP), and boron at 3, 5, and 7 WAP. The results showed that application of BAP improved flowering, pollen viability, and seed production of TSS but did not improve seed quality, while boron application effectively increased all variables including the seed quality of TSS. The efficient concentration of BAP to improve TSS seed weight per plot was 37.5 ppm, while the optimum concentration of boron to improve TSS seed production with the good seed quality (according to the certification standards of seed quality) was 2.88 kg/ha. The yield obtained in the treatment of boron gave an increase of 165.69% compared to the control. The results provide information about TSS production techniques that can be developed to produce the high seed quality of TSS.<br /><br />
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Yuniastuti, Endang, Praswanto Praswanto, and Ika Harminingsih. "PENGARUH KONSENTRASI BAP TERHADAP MULTIPLIKASI TUNAS ANTHURIUM (Anthurium andraeanum Linden) PADA BEBERAPA MEDIA DASAR SECARA IN VITRO." Caraka Tani: Journal of Sustainable Agriculture 25, no. 1 (2017): 1. http://dx.doi.org/10.20961/carakatani.v25i1.15476.
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<p>Penelitian bertujuan untuk mengetahui pengaruh macam media tanam dan konsentrasi BAP (6-Benzyl Amino Purine) terhadap multiplikasi tunas Anthurium secara in vitro. Perlakuan penelitian meliputi macam media tanam yang terdiri dari : medium Murashige dan Skoog (MS), Schenk dan Hildebrandt (SH) serta Nitsch dan Nitsch (N2) dan konsentrasi BAP terdiri dari : 0 ppm, 2 ppm, 4 ppm. Variabel pengamatan meliputi saat muncul tunas, jumlah tunas, panjang tunas, saat muncul daun, jumlah daun, saat muncul akar, panjang dan jumlah akar. Rancangan penelitian berupa Rancangan Acak Lengkap (RAL). Data yang didapat ditampilkan secara diskriptif dan uji F taraf 5%, bila ada beda nyata dilanjutkan uji DMRT. Hasil penelitian menunjukkan bahwa terjadi interaksi antara media dan konsentrasi BAP secara nyata pada jumlah dan panjang tunas. Medium N2 dengan penambahan 4 ppm BAP menunjukkan adanya jumlah tunas terbanyak dan tunas terpanjang. Medium MS tanpa penambahan BAP menunjukkan saat muncul tunas tercepat. Saat muncul daun tercepat pada perlakuan MS dengan 2 ppm BAP dan jumlah daun terbanyak ditunjukkan pada perlakuan medium SH tanpa penambahan BAP. Saat muncul akar tercepat pada perlakuan medium SH dengan 4 ppm BAP, sedangkan akar terpanjang ditunjukkan oleh perlakuan Medium N2 tanpa penambahan BAP.</p>
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Meli, Husri, Zozy Aneloi Noli, and Suwirmen Suwirmen. "Induksi Kalus Tanaman Puspa (Schima wallichii (DC.) Korth) dengan Penambahan Beberapa Konsentrasi Benzyl Amino Purin (BAP) dan 2,4-Diklorofenoksiasetat (2,4-D)." Jurnal Biologi UNAND 7, no. 1 (2019): 1. http://dx.doi.org/10.25077/jbioua.7.1.1-5.2019.
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The research about Callus Induction of Puspa (Schima wallichii (DC.) Korth) with several concentrations addition of Benzyl Amino Purine (BAP) and 2,4-Dichlorophenoxyacetid acid (2,4-D) had been done from October until November 2016 at Plant Physiology dan Tissue Culture Laboratory, Biology Department, Faculty of Mathematics and Natural Science, Andalas University. The aim of this researh is to get the combination of 2,4-D and BAP to induce the best callus of Schima wallichii. This research used a Completely Randomized Design Method with 10 treatments and 3 replications. The result showed that combination of 2 ppm 2,4-D + 0,75 ppm BAP was the best concentration to induce callus of Schima wallichii.
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Sivakumar, R., and K. Krishna Surendar. "Effects of plant growth regulators and potassium on growth and yield of finger millet." Bangladesh Journal of Botany 49, no. 2 (2020): 223–28. http://dx.doi.org/10.3329/bjb.v49i2.49295.
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Impact of plant growth regulators viz., benzyl amino purine (BAP - 50 ppm), brassinolide (BL - 0.5 ppm), and nutrient like KCl (1%) alone and with combination on growth and growth analytical parameters, and yield of finger millet by foliar spray at panicle initiation stage under rainfed condition was studied in field experiment. The treatments including control, KCl, BAP, BL, KCl + BAP, KCl + BL, BAP + BL and KCl + BAP + BL and different growth and growth analytical parameters were estimated. Combination of KCl, BAP and BL registered highest root length (17.2 cm), leaf area (425.3 cm2), leaf area index (1.82) and leaf area duration (55.7 days) compared to other treatments. Higher specific leaf weight and crop growth rate were recorded by KCl + BAP, and BAP + BL, respectively. The highest grain yield of 22.5 Q/ha was recorded by KCl + BAP + BL.
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Charantika, J. W., Samanhudi, and A. T. Sakya. "Organic materials and fish emulsion on shoot multiplication of Cavendish banana (Musa paradisiaca ‘Cavendish’)." IOP Conference Series: Earth and Environmental Science 1114, no. 1 (2022): 012109. http://dx.doi.org/10.1088/1755-1315/1114/1/012109.
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Abstract This study aims to investigate the effect of combining organic matter and fish emulsion on the multiplication of Cavendish banana shoots. This study employed one factor of a complete randomized design, with 13 levels of treatment: MS (Murashige and Skoog) + purple sweet potato extract, MS + purple sweet potato extract + fish emulsion 2 ppm, MS + purple sweet potato extract + fish emulsion 4 ppm, MS + purple sweet potato extract + fish emulsion 6 ppm, MS + banana peel extract, MS + banana peel extract + fish emulsion 2 ppm, MS + banana peel extract + fish emulsion 4 ppm, MS + banana peel extract + fish emulsion 6 ppm, MS + bean sprouts extract, MS + Bean sprouts extract + fish emulsion 2 ppm, MS + bean sprouts extract + fish emulsion 4 ppm, MS + bean sprouts extract + fish emulsion 6 ppm, and MS + BAP (benzyl amino purine) as controls. The results show that the combination of synthetic materials (BAP) still has significantly different results compared to organic matter and fish emulsion treatment.
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Naser, Ahmad Saifun, and Muhammad Wisnu. "Callus Induction of Leaves and Stems in Krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) with Alternative Foliar Fertilizers Media." Biology, Medicine, & Natural Product Chemistry 9, no. 2 (2021): 109–15. http://dx.doi.org/10.14421/biomedich.2020.92.109-115.
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Availability of quality seeds in production of krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) cultivation is still rare, therefore research on seed multiplication through tissue culture is needed. The media used in tissue culture is relatively expensive for home industry. This study aims to determine the respond of leaf and stem explants using foliar fertilizers (Growmore, Gandasil D and Mutiara) as an alternative media for callus inductions. This study used a Completely Randomized Design (CRD) consisted of 4 treatments: P0: ½ MS + 0,25 mg/l BAP, P1 (Growmore + 0,25 mg/l BAP), P2 (Gandasil D + 0,25 mg/l BAP), P3 (Mutiara + 0,25 mg/l BAP). The variables observed in this study included callus appearance time, callus color and callus texture. The result of this study indicated that the use of BAP (6-Benzyl Amino Purine) affected the time of callus formation and callus morphology. Callus was formed on leaf explants 13 days after planting while on stem explants 7 days after planting and compact texture. Growmore + 0,25 mg/l BAP treatment yields the best callus on leaf explant, while Gandasil D + 0,25 mg/l BAP treatment yields the best callus on stem explant.
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Jannah, Kennaya Putri Aura, Iwan Prihantoro, and Panca dewi manu hara Karti. "Optimasi Level Benzyl Amino Purin (BAP) terhadap Pertumbuhan Tanaman Kembang Telang (Clitoria ternatea) melalui Teknik Kultur Jaringan." Jurnal Ilmu Nutrisi dan Teknologi Pakan 21, no. 2 (2023): 100–106. http://dx.doi.org/10.29244/jintp.21.2.100-106.
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The research objective was to measure the optimum level of BAP for supporting the growth of butterfly pea flowers through tissue culture techniques. The study used a completely randomized design (CRD) with 5 treatments based on BAP media levels (BAP 0 ppm, BAP 0.5 ppm, BAP 1 ppm, BAP 1.5 ppm, and BAP 2 ppm) and 20 replications. Parameters measured were plant height, plant height increase, number of leaves, number of branches, number of tillers, percentage of tiller growth, plant weight, and leaf color. The results showed that the use of BAP levels 0, 0.5, 1, 1.5, and 2 ppm in butterfly pea flower plants through tissue culture techniques was not effective in increasing plant height, number of leaves, number of branches, number of tillers, and percentage of tillers at the end of the research (27 DAP). Using an optimum BAP level of 0.5 ppm resulted in higher biomass production and the dominance of green leaf color visualization.
 Key words: BAP (Benzyl Amino Purine), butterfly pea, Clitoria ternatea, tissue culture
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Rani, Archana, M. Kumar, and Sanjeev Kumar. "In vitro propagation of Withania somnifera (L.) Dunal. from shoot apex explants." Journal of Applied and Natural Science 6, no. 1 (2014): 159–63. http://dx.doi.org/10.31018/jans.v6i1.393.
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In vitro rapid micropropagation of Withania somnifera; has been achieved using shoot apex explants, when cultured on MS medium with different auxins: Indole Butyric Acid (IBA) 0.5-4 mg/l, Indole Acetic Acid (IAA) 2 mg/l and Naphthalene Acetic Acid (NAA) 0.5-3 mg/l and cytokinin: Benzyl Amino Purine (BAP) 0.5-4 mg/l. NAA and BAP stimulated direct as well as callus mediated multiple shoot. IBA alone and in combination with IAA stimulated rhizogenesis, while IBA with NAA proliferated callus-mediated rhizogenesis. The rooted plantlets through sequential hardening process could be established in the field.
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M, Mataa, Musenga C, and Hakachite C. "Shelf life responses of 'Akito' rose (Rosa spp.) cut flowers treated with growth regulator benzyl amino purine and microbiocide aluminium sulphate." International Journal of Agricultural Research, Innovation and Technology 10, no. 1 (2020): 6–12. https://doi.org/10.3329/ijarit.v10i1.48088.
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Rose (Rosa spp.) production for cut flowers is an integral part of the horticultural industry. However, because roses are exotic plants they exhibit serious problems related to poor climatic adaptation. A study was conducted at the School of Agricultural Sciences, University of Zambia to evaluate chemicals to extend flower shelf life. The cultivar ‘Akito’ was used as the test variety. Benzyl amino purine ([6- (benzylamino) purine]) (BAP), a growth regulator and aluminium sulphate (an acidifying reagent) were applied to cut flowers at 0, 5, 10, 15, and 20 mg L-l and 0, 400, 800 and 1200 mg L-l, concentration, respectively. BAP was applied to the plants in the greenhouse 2 days before harvest. A randomised complete block design arranged as a two- factor- factorial arrangement with three replications was used. Harvesting was done at the loose open calyx stage. Aluminium sulphate was applied to the holding solution where flowers were kept after harvest. The pH of the holding solution, blossoming (flower opening), leaf color of subtending leaves and bacterial population were monitored. The 400 mg L-l aluminium sulphate treatment caused an increase in acidity. There was concurrent decline in bacterial count in the first three days of application at less than 1 x 106 which was five times lower than the water control treatment. Bacterial population followed the trend of water acidity. The 20 mg L-l BAP application increased shelf life by 35 %. The results showed that BAP and aluminum sulphate treatment could enhance shelf life.
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Wardana, Rudi, Ana Uzunul Mauidah, Jumiatun Jumiatun, Tirto Wahyu Widodo, and Refa Firgiyanto. "Multiplikasi Tunas Kentang Merah (<i>Solanum Tuberosum</i> L.) dengan Aplikasi ZPT NAA Dan BAP Secara In Vitro." Vegetalika 13, no. 4 (2024): 383. https://doi.org/10.22146/veg.95287.
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Rendahnya produksi kentang merah di Indonesia dikarenakan terbatasnya ketersediaan bibit unggul dan bibit rentan terhadap serangan patogen. Perlu adanya solusi alternatif untuk meningkatkan mutu bibit kentang merah yang seragam dan bebas patogen yaitu melalui teknik kultur in vitro. Penelitian ini bertujuan untuk menganalisis konsentrasi terbaik NAA dan BAP terhadap multipikasi tunas kentang merah secara in vitro. Penelitian ini dilakukan di Laboratorium Kultur Jaringan Politeknik Negeri Jember. Rancangan percobaan menggunakan metode Rancangan Acak Lengkap Faktorial (RALF) yang terdiri dari atas dua faktor. Faktor pertama adalah konsentrasi Napthalene Acetic Acid (NAA) meliputi 0,1 mg/l; 0,3 mg/l; 0,5 mg/l. Faktor kedua adalah konsentrasi 6-Benzyl Amino Purine (BAP) meliputi 1 mg/l; 1,5 mg/l; 2 mg/l). Hasil penelitian menunjukkan bahwa aplikasi kombinasi konsentrasi 0,3 mg/l NAA dan 1,5 mg/l BAP berpengaruh berbeda nyata terhadap tinggi tunas (7,03 cm). Aplikasi konsentrasi NAA 0,1 mg/l berpengaruh berbeda nyata terhadap waktu munculnya kalus (9,44 HST). Pada aplikasi konsentrasi BAP 1,5 mg/l berpengaruh berbeda nyata terhadap waktu muncul tunas yaitu 8,78 HST dan jumlah daun (7,58 helai).
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Rana, Syafia Diang, Reza Puspita Dewi, Agung Purnomo Adjie, and Mayta Novaliza Isda. "Respons Poliembrioni Dari Biji Duku (Lansium domesticum Corr.) yang Dibelah Tiga Secara In Vitro." Journal of Biota 4, no. 2 (2019): 63. http://dx.doi.org/10.24002/biota.v4i2.2472.
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Duku (Lansium domesticum Corr.) adalah salah satu buah tropis bernilai ekonomis yang diminati oleh masyarakat dengan rasa manis dan bernilai gizi tinggi. Biji duku memiliki sifat poliembrioni yang akan menghasilkan tanaman lebih banyak dan seragam sesuai dengan induknya. Perbanyakan dengan teknik in vitro akan menghasilkan tanaman dalam jumlah yang banyak dalam waktu yang relatif singkat dibandingkan secara konvensional. Tujuan penelitian yaitu untuk mengetahui respon poliembrioni biji duku yang dibelah tiga secara membujur dengan penambahan BAP (Benzyl Amino Purine) secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan konsentrasi BAP = 0, 1, 3, 5 dan 7 mg/L BAP. Hasil penelitian menunjukkan bahwa waktu muncul tunas tercepat yaitu 2,20 MST pada konsentrasi 1 mg/L BAP. Jumlah tunas tertinggi pada perlakuan 3 mg/L BAP yaitu 2,00 tunas, namun perlakuan pemberian konsentrasi BAP belum mampu meningkatkan jumlah tunas.
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Pratiwi, Berlina Intan, Pangesti Nugrahani, and Nora Augustien K. "Pengaruh Nutrisi AB Mix dan Benzyl Amino Purine (BAP) terhadap Pertumbuhan Pisang (Musa acuminata) Var. Cavendish In Vitro." Agro Bali : Agricultural Journal 6, no. 1 (2023): 231–40. http://dx.doi.org/10.37637/ab.v6i1.1163.
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Nutrisi AB Mix dan Zat Pengatur Tumbuh (ZPT) BAP merupakan salah satu perlakuan untuk merangsang pertumbuhan planlet pisang Cavendish. Tujuan penelitian adalah untuk mengetahui pengaruh konsentrasi AB Mix dan BAP terhadap pertumbuha planlet pisang Cavendish (Musa acuminata). Penelitian dilaksanakan di Laboratorium Bioteknologi Fakultas Pertanian UPN Veteran Jawa Timur. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL) Faktorial dengan 2 faktor perlakuan yang diulang 3 kali. Faktor pertama adalah konsentrasi AB Mix (M) yang terdiri dari 4 taraf yaitu MS (M0), AB Mix 1 ml.l-1 (M1), AB Mix 3 ml.l-1 (M2), dan AB Mix 5 ml.l-1 (M3) dan konsentrasi BAP (B) sebagai faktor kedua yang terdiri dari 4 taraf yaitu BAP 0 ppm (B0), BAP 1,5 ppm (B1), BAP 3 ppm (B2), BAP 4,5ppm (B3), sehingga didapatkan 16 perlakuan kombinasi. Hasil jumlah tunas yang lebih banyak diperoleh pada perlakuan AB Mix 5 ml.l-1 dan BAP 4,5 ppm, jumlah daun yang lebih banyak diperoleh pada perlakuan AB Mix 5 ml.l-1, jumlah akar yang lebih banyak diperoleh pada perlakuan AB Mix 5 ml.l-1 dan BAP 1,5 ppm.
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Zanirah, Shofiyah, Sutini Sutini, and Didik Utomo Pribadi. "The Effect of Growmore and BAP (Benzyl Amino Purine) Concentrations on The Growth of Dendrobium bigiante agrihorti Orchid In-Vitro." Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) 12, no. 3 (2023): 710. http://dx.doi.org/10.23960/jtep-l.v12i3.710-720.
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The increase in production of Dendrobium bigiante agrihorti orchids by in vitro culture is to produce numerous and uniform seedlings. This study aimed to determine the effect of Growmore and BAP concentrations on the growth of orchid plantlets. The experiment consisted of two factors namely Growmore concentration (0; 3; 6; 9 ppm), and BAP concentrations (0; 1; 3; 5 ppm). The factors combination on ½ MS media was repeated 3 times and obtained 48 experimental units. Observation parameters included the percentage of live plantlets, number of roots, number of leaves, plant height (cm), and wet weight of plantlets (g). Results showed that a Growmore concentration of 3 ppm had a significant effect on the number of roots and plant height at 4.36 units and 4.17 cm. BAP concentration of 3 ppm also had a significant effect on the number of roots, plant height, and wet weight of respectively 4.72 units, 3.22 cm, and 0.63 g. A combination of Growmore 3 ppm + BAP 0 ppm produced the largest percentage of live planets at 100% and plant height at 7.37 cm, Growmore 9 ppm + BAP 0 ppm produced the largest number of roots at 6.33 units and Growmore 6 ppm + BAP 3 ppm produced the largest number of leaves at 8.78 strands. Keywords: BAP, Dendrobium bigiante, Growmore.
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Ilmiyah, Isnanda Rosyidatul, Maftuchah, and Muhidin. "Effect Of Bap (Benzyl amino purine) Concentrations on Shoot Multiplication Of Two Varieties Of Kepok Banana In Vitro." Journal of Tropical Crop Science and Technology 4, no. 1 (2022): 27–48. http://dx.doi.org/10.22219/jtcst.v4i1.29747.
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Banana production in Indonesia has decreased due to the lack of availability of healthy seeds. The purpose of this study was to determine the effect of BAP on the multiplication of two banana varieties (Kepok Manurun and Kepok Unti Sayang) by in vitro culture. This study used a completely randomized design (CDR) in a factorial manner with 2 factors. The first factor was the banana variety (Kepok Manurun and Kepok Unti Sayang), the second factor was the concentration of BAP ZPT (0, 1, 2, 3) with 4 replications where each replication consisted of 4 samples, each bottle was planted with 1 plantlet for a total of 128 bottles. The results showed that the concentration of BAP did not effect the multiplication of two kepok banana varieties in vitro. The BAP concentration of 2 ml/l produced the highest number of shoots of 1,91 shoots, the concentration of BAP of 1 ml/l produced the highest number of leaves of 1,52 leaf, while the concentration of BAP 0 ml/l or without BAP result in the highest plant height of 1,05 cm. While the highest number of roots was found in the BAP treatment of 1 ml/l, the number of root tips, the highest root length was found in the BAP treatment 0 ml/l, while the concentration of BAP 2 ml/l produced the highest number of tillers of 1,39 tillers, and the concentration BAP 1 ml/l resulted in a stem diameter of 3,98 cm.
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Hidayatullah, Careca Sepdihan Rahmat, Untung Santoso, Fatimah Nursandi, Rich Gemilang Simanjuntak, and Oentari Priliraningrum. "Evaluasi Kultivar Apel dan Zat Pengatur Tumbuh (Sitokinin-Auksin) Terhadap Tingkat Keberhasilan Eksplan Apel Secara In Vitro." Agrocentrum 2, no. 2 (2024): 82–90. https://doi.org/10.33005/agrocentrum.v2i2.45.
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Successful in vitro propagation can support the provision of large, fast and highquality apple seeds. Growth regulators commonly used in culture techniques are cytokinins and auxins. the addition of 4 ppm BAP to the culture media can increase the lipid content and biomass 1.26 times higher in microalgae. This study aims to evaluate the ability of apple cultivars and the growth regulators BAP (Benzyl amino purine) and IAA (Indole acetic acid) to regenerate apple shoots in vitro. The experimental design used RAK (randomized block design) 2 factors. The first factor was shoots from four apple cultivars, namely Fuji, Red delicious, gala, and manalagi. The media used were 1) MS + 3 mg/L BAP + 0.2 mg/L IAA, 2) MS + 6 mg/L BAP + 0.2 mg/L IAA, 3) MS + 3 mg/L BAP + 0.3 mg/L IAA, and 4) MS + 6 mg/L BAP + 0.3 mg/L IAA. Based on the percentage of live explants, apple shoots show a relatively high level of success in the Fuji apple cultivar for all BAP-IAA growth regulator compositions, namely 3 mg/L BAP + 0.2 mg/L IAA, 6 mg/L BAP + 0.2 mg/L IAA, 3 mg/L BAP + 0.3 mg/L IAA, and 6 mg/L BAP + 0.3 mg/L IAA. Apple cultivars and growth regulators did not show a significant effect on the percentage of live explants, green color of shoots, explant contamination and number of leaves. There is a need for further studies regarding the type of explant with the right type of cytokinin hormone to regenerate green shoots more optimally.
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shakira, Afnan Mohammad Sadar, Madhaiyan Sowmiya, Mahadevan Sneaha, et al. "Validation of BAP (6 – Benzyl Amino Purine) Concentration on the Micropropagation of Rose through Shoot Tip Culture." International Journal of Innovative Science and Research Technology 7, no. 7 (2022): 1189–92. https://doi.org/10.5281/zenodo.6987919.
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In this study, the nodal explants collected from the healthy shoots were cultured on the basal MS medium supplemented with 3mg/lit of BAP (6-Benzyl Amino Purine). In the earlier study, several researchers reported that the concentration of 3mg/lit is optimum for the growth and development of shoot tip in rose by the duration of 1month. The different stage of shoot development was observed from the third day to one month interval. It revealed that the callus initiation starts from the 10th day and auxillary shoot initiation from 2 weeks, and subsequent growth and development was observed from the third week and one month interval respectively. Further the developed healthy shoot culture will be maintained and will be treated with NAA at different concentration for root initiation. Finally, the optimum concentration of NAA will be fixed for better root initiation and development.
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Siregar, Dolly Sojuangan, and Muhammad Azmi Baihaki. "Growth Response of Potato (Solanum tuberosum L.) to Application of Indole Acetic Acid (IAA) and Benzyl Amino Purine (BAP) by In Vitro." JURNAL AGROTEKNOLOGI 13, no. 1 (2025): 15–19. https://doi.org/10.32734/ja.v13i1.19880.
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Potatoes are one of the important food crops in the world and a source of carbohydrates. However, providing seeds is still a problem due to their vulnerability to pests and disease. Technique tissue culture is an alternative for propagating Granola potato varieties. Influence giving concentrations of Indole Acetic Acid (IAA) and Benzyl Amino Purine (BAP) on growth potato (Solanum tuberosum L.) in vitro. This research was carried out in the Tissue Culture Laboratory Alifa Agricultural Research Center (ALIFA-ARC) Medan. This research was conducted in February until May 2024. This research used a Completely Randomized Design (CRD) method with 2 factors treatment. The first factor is the concentration of Indole Acetic Acid (I): IO (0 mg/l), I1 (0,2 mg/l), I2 (0,3 mg/l). The second factor is the concentration of Benzyl Amino Purine (B): B0 (0 mg/l), B1 (2 mg/l), B2 (3 mg/l). Parameter observed in this study included, percentage of live explants, percentage of dead explants, time to emergence shoots, number of shoots, length of axillary shoots, number of roots, root length, and callus weight. The result shows that the combination of IAA and BAP has a significant effect on all observation parameters. The best treatment for shoot growth was found in I0B2, root growth in treatment I2B0, and callus growth on I2B2.
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Setiowati, Frida Kunti, and Nina Aulia Rahmah. "Pengaruh Penambahan Kombinasi Benzyl Amino Purine dan Pepton Terhadap Pertumbuhan Planlet Anggrek Cattleya sp." Jurnal Ilmu Hayat 7, no. 1 (2024): 43. http://dx.doi.org/10.17977/um061v7i12023p43-51.
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Orchidaceae is one type of ornamental plant which is a leading commodity. Cattleya sp. are a potential type of orchid in terms of productivity and high economic value. Conventional Cattleya sp. cultivation is considered less effective because the number of tillers produced is very limited. Therefore an alternative Cattleya sp. cultivation is needed using plant tissue culture techniques or in vitro. The research objective is to determine the effect of the addition of the combination of BAP and peptone on the growth of orchid plantlets Cattleya sp. This experiment was conducted in the Plant Tissue Culture Laboratory of the Faculty of Mathematics and Natural Science Universitas Negeri Malang from August to November 2016. This study used a completely randomized design. The treatment was in the form of ½ MS medium added with a combination of Benzyl Amino Purine (BAP) and Peptone. The explant used was the protocorm of Cattleya sp. 1 month old with a size of ± 0.5 cm. Protocorm was inoculated as much as 10 protocorm on media treatment. The results showed that Peptone can affected the growth of Cattleya sp. plantlets. The ½ MS treatment with the addition of peptone produced the highest number of leaves and the highest plantlet height, while the ½ MS treatment without the addition of BAP and peptone produced the highest number of roots.
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Syamsiah, Melissa, Angga Adriana Imansyah, Hana Khoerunisa Suprapti, and Dedeh Siti Badriah. "RESPON MULTIPLIKASI ANGGREK BULAN (Phalaenopsis sp.) TERHADAP PENAMBAHAN BEBERAPA KONSENTRASI BAP (Benzyl Amino Purine) PADA MEDIA IN VITRO." AGROSCIENCE (AGSCI) 10, no. 2 (2020): 148. http://dx.doi.org/10.35194/agsci.v10i2.1157.
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Jenis anggrek yang paling banyak diminati dan dibudidayakan adalah anggrek bulan (Phalaenopsis sp.). Perbanyakan anggrek bulan dapat dilakukan dengan metode alternatif yang lebih efektif yaitu dengan melalui kultur in vitro. Tujuan penelitian adalah mengetahui pengaruh penambahan beberapa konsentrasi BAP (Benzyl Amino Purine) terhadap multiplikasi, waktu muncul tunas (HST), jumlah tunas, waktu muncul daun (MST), dan jumlah daun anggrek bulan (Phalaenopsis sp.). Penelitian dilakukan di Laboratorium Pemuliaan Kebun Percobaan Tanaman Hias Cipanas Balai Penellitian Tanaman Hias (BALITHI) dari bulan Februari – Mei 2020. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan perlakuan beberapa konsentrasi BAP : B0 (0 mg/Liter), B1 (0,50 mg/Liter), B2 (1,00 mg/Liter), B3 (1,50 mg/Liter), B4 (2,00 mg/Liter). Hasil penelitian penambahan BAP berpengaruh positif terhadap waktu muncul tunas, waktu muncul daun dan jumlah daun tanaman anggrek. Penambahan BAP dengan konsentrasi 2,00 mg/L merupakan perlakuan paling baik. Sedangkan pengaruh terhadap parameter jumlah tunas tanaman anggrek bulan ditunjukkan oleh perlakuan penambahan BAP dengan konsentrasi 1,50 mg/L.
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Krisnawati, Endang. "PENGARUH WAKTU PEMBERIAN DAN KONSENTRASI BENZYL AMINO PURINE (BAP) TERHADAP PERTUMBUHAN TANAMAN KEDELAI (Glycine max (L.) Merrill)." Jurnal Penyuluhan Pertanian 3, no. 1 (2020): 79–87. https://doi.org/10.51852/jpp.v3i1.247.
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Penelitian ini bertujuan untuk mengetahui pengaruh waktu pemberian dan konsentrasiBenzyl Amino Purine (BAP) terhadap pertumbuhan dan hasil tanaman kedelai. Penelitianmenggunakan Rancangan Acak Kelompok Lengkap Faktorial terdiri dari tiga faktor dan tigaulangan. Faktor pertama adalah varietas yaitu Malabar dan Argo Mulyo. Faktor kedua adalahwaktu pemberian BAP terdiri dari tiga taraf : pada saat 15 hari setelah tanam (W1), saat inisiasibunga (W2), dan saat 15 hari setelah tanam dan inisiasi bunga (W3). Faktor ketiga adalahkonsentrasi BAP terdiri dari tiga taraf: 0, 50, dan 100 ppm, sehingga terdapat 54 satuanpercobaan.Hasil penelitian menunjukkan bahwa Varietas Malabar memberikan pengaruh yanglebih baik, yang ditunjukkan oleh jumlah daun yang lebih banyak (33.3 helai) dari padavarietas Argo Mulyo (28.6 helai), jumlah biji lebih banyak, bobot biji per tanaman lebih berat,dan bobot biji per petak lebih berat. Waktu pemberian BAP dengan dua kali pemberian yaitupada umur 15 hari setelah tanam dan pada saat inisiasi bunga mampu meningkatkan jumlahklorofil (41.64 per mm2), bobot 100 biji (18.39 g).Konsentrasi BAP sampai 100 ppm mampu meningkatkan jumlah daun (32.9 helai),jumlah klorofil (44.29 per mm2), jumlah biji per tanaman (168.0 butir), bobot 100 biji (18.81g), bobot biji per tanaman (30.93 g), dan bobot biji per petak.
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Triani, Nova, Pangesti Nugrahani, and Elly Syafriani. "INDUKSI TUNAS TIN (Ficus carica L.) SECARA IN VITRO." BERKALA ILMIAH AGROTEKNOLOGI - PLUMULA 6, no. 2 (2019): 86–92. http://dx.doi.org/10.33005/plumula.v6i2.6.
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Penelitian ini bertujuan untuk mempelajari pengaruh (Benzyl Amino Purine) BAP dan air kelapa pada media Murashige and Skoog (MS) pada induksi tunas dari pucuk tanaman Tin. Pengamatan dilakukan pada pemberian 1 ppm dan 2 ppm BAP dan 100 ml/l dan 150 ml/l air kelapa pada media MS yang menggunakan arang aktif dan tidak menggunakan arang aktif. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 9 perlakuan, yaitu MS0, MS+BAP 1 ppm, MS+BAP 2 ppm, MS+BAP 1 ppm+arang aktif, MS+BAP 2 ppm+arang aktif, MS+air kelapa 100ml/l, MS+air kelapa 150 ml/l, MS+air kelapa 100 ml/l+arang aktif, and MS+air kelapa 150 ml/l. Pucuk tanaman tin dikultur pada media MS yang diperkaya dengan 30 mg/l gula untuk proliferasi tunas secara in vitro. Hasil yang diperoleh yaitu media MS yang ditambah dengan BAP 1 ppm+arang aktif, meningkatkan perkembangan tunas. Pada penelitian ini masih banyak eksplan yang mati karena pencoklatan dan kontaminasi.
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Arafah, Dila Lailatul, Diana Hernawati, and Egi Nuryadin. "The Effect Hormone BAP (6-Benzyl Amino Purine) on the Growth of Potato Axillary Shoots (Solanum Tuberosum L.) in Vitro." Jurnal Biologi Tropis 21, no. 3 (2021): 641–47. http://dx.doi.org/10.29303/jbt.v21i3.2823.
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Potatoes are one of the foodstuffs that contain lots of carbohydrates, minerals, and vitamins. To develop potato cultivation, superior varieties are needed that produce high yields, are resistant to pests, and have good tuber quality. The growth of potato plantlets in the in vitro subculture process is determined by many factors, one of which is the administration of growth regulators and the concentration of growth regulators. The type of media or nutrients used also affects the growth and development of the explants and seedlings produced. This study aims to trigger the growth of potato plantlets so that their growth is optimal. The method used is a true experimental quantitative method with a one-factor Completely Randomized Design (CRD) design, namely variations in BAP concentration consisting of 5 treatments with 5 replications. Data collection techniques through observation or observation. Parameters observed were number of shoots, number of leaves, number of roots, and plantlet height. The data analysis technique used is the one way ANOVA test and for the average difference between treatments, the HSD (Honestly Significant Difference) test at 5% level is used. Based on the results of the study, it was known that there was an effect of giving the hormone BAP (6-Benzyl Amino Purine) on the growth of potato axillary shoots (Solanum tuberosum L.) in vitro. The results showed that there was a significant effect on the number of shoots (P = 0.007) with an average of 1.64 pieces, the number of leaves (P = 0.002) with an average of 1.42 pieces, the number of roots (P = 0.000) with an average of 1. an average of 1.70 pieces, and plantlet height (P=0.000) with an average of 2.65 cm. It was concluded that treatment D (1.5 ppm BAP) was the best treatment in increasing the growth of potato plantlets.
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ANIEL KUMAR, Owk, Songa RAMESH, and Sape SUBBA TATA. "In Vitro Micropropagation of the Medicinal Plant Physalis angulata L." Notulae Scientia Biologicae 8, no. 2 (2016): 161–63. http://dx.doi.org/10.15835/nsb829817.
Full textAbstract:
Physalis angulata L. is an important medicinal herb. An efficient direct adventitious plant regeneration protocol was developed for large scale propagation using leaf disc as explants. The explants were cultured on MS basal medium supplemented with 0.25-3.0 mg/L 6-benzyl amino purine (BAP) for primary shoot proliferation. Inclusion of indole-3-acetic acid (IAA) and gibberellic acid (GA3) in the culture medium along with BAP promoted a higher rate of shoot multiplication. The maximum number of shoots was produced in MS + BAP (1.0 mg/L) + IAA (0.5 mg/L) + GA3 (0.20 mg/L) after the third subculture. An average of 152.8 ± 0.40 shoots were produced from each leaf disc. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of indole-3-butyric acid (IBA). The highest percentage of root induction was observed in 1.0 mg/L (IBA). Rooted plants were successfully established in the soil after hardening. The survival percentage of rooted plants on soil was found to be 85%. This result will facilitate the conservation and propagation of the important medicinal herb Physalis angulata L.
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Ramadhan, Mochamad Alif, Fawzy Muhammad Bayfuqron, Nurcahyo Widyodaru Saputro, and Sri Suhesti. "Pengaruh Kombinasi BAP (Benzyl Amino Purine) dan Kinetin Terhadap Multiplikasi Tunas Tanaman Tebu (Saccharum officinarum L.) Varietas AAS Agribun." JURNAL AGROPLASMA 11, no. 1 (2024): 153–60. http://dx.doi.org/10.36987/agroplasma.v11i1.5342.
Full textAbstract:
Sugarcane (Saccharum officinarum L.) is a plantation commodity crop and raw material for making sugar. The need for sugar increases every year so it is necessary to provide superior sugarcane seeds in large quantities in a relatively short time. This can be done by propagating using the plant tissue culture method. The research method used was an experimental method using a combined Completely Randomized Design (CRD) with 4 replications and 8 treatments consisting of: Control (P0); 0.5 ppm BAP + 0 ppm kinetin (P1); 1 ppm BAP + 0 ppm kinetin (P2); 2 ppm BAP + 0 ppm kinetin (P3); 0 ppm BAP + 0.1 ppm kinetin (P4); 0.5 ppm BAP + 0.1 ppm kinetin (P5); 1 ppm BAP + 0.1 ppm kinetin (P6); and 2 ppm BAP + 0.1 ppm kinetin (P7). The data then explained the variations and then continued with the DMRT test at the 5% level. The results showed that treatments P3 and P7 showed the best results at the time of shoot emergence. The P3 treatment also showed the best results in the number of shoot (8.25) and number of leaves (16.50). The P7 treatment showed the highest shoot yield (4.58). Keywords: sugarcane, BAP, kinetin, shoot multiplication