Academic literature on the topic '6-Furfurylaminopurine'

The crystal structure of kinetin dihydrogenphosphate has been determined at 115 and 293 K. Kinetin dihydrogenphosphate undergoes a polymorphic phase transition at 291.1 K. In both phases the crystal belongs to the triclinic system with the symmetry described by the space group P\bar 1. In the low-temperature phase, the unit cell is doubled along the a axis. There is a dynamic equilibrium between different tautomeric forms of the adenine residue, determined by the distribution of H atoms within the network of hydrogen bonds.

Embryos from imbibed ripe seeds and cotyledon expiants of 7-day-old Norway spruce (Piceaabies (L.) Karst.) seedlings produced the early stages of somatic embryogenesis. Using a modified Murashige and Skoog medium, a whitish, glossy callus was induced consisting of translucent cells embedded in a mucilaginous cloudy matrix. This embryogenic callus formed on the surface of explants treated first with N-6-benzyladenine followed by 2,4-dichlorophenoxyacetic acid + 6-furfurylaminopurine (kinetin) + N-6-benzyladenine. Transfer of this callus to media lacking growth regulators resulted in the formation of numerous bipolar embryoids with suspensorlike structures. These embryoids strongly resembled repressed embryos in polyembryonic seeds.

Both kinetin and BA promoted in vitro shoot formation from hypocotyl explants of Lupinus texensis Hook. placed on Murashige and Skoog (MS) medium. With either cytokinin, shoot formation was best at ≈4.5 μm. Adventitious root formation was observed only on tissue culture-derived shoots placed in MS media containing 5.4 to 54 μM NAA. IAA and IBA, at concentrations ranging from 5 to 55 μm, failed to stimulate rooting. Even at the optimal concentration of NAA, only 14% of the shoots produced roots. Thus, although hypocotyl explants readily produced shoots, adventitious root formation on these shoots occurred with relatively low frequency. Chemical names used: 6-benzylaminopnrine (BA); indole-3-acetic acid (IAA); indole-3-butyric acid (IBA); 6-furfurylaminopurine (kinetin); 1-naphthaleneacetic acid (NAA).

Micropropagation methods for six rockrose species (Cistus albidus L., C. clusii Dunal, C. ladanifer L., C. laurifolius L., C. psilosepalus L., and C. salvifolius L.) were established. Cultures, initiated from nodal segments of seedlings, were grown on MS medium, alone, or supplemented with 0.88 μm BAP or 0.93 μm Kin. Multiple shoot formation was obtained after the first subculture (30 days) from which new nodal segments were taken and grown on the same culture medium to maintain proliferation. Shoots obtained at the third subculture were rooted alone or supplemented with different concentrations of IBA. The plantlets of the six species, thereby obtained, were successfully acclimatized to ex vitro conditions. Chemical names used: 6-benzylaminopurine (BAP), indole-3-butyric acid (IBA), 6-furfurylaminopurine (Kin).

Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7 day-old seedlings were incubated on Murashige and Skoog's (MS) medium supplemented with 1 mg l-1 6-benzyladenine (BA) or 6-furfurylaminopurine (KIN) in combination with 0, 0.1, and 0.2 mg l-1 indole-3-butyric acid (IBA). Hypocotyls showed the best explants in almost all varieties tested with a minimum regeneration potential of 75% and producing 3.5-7.4 shoots per explant. The BA-supplemented media were optimal for both shoot regeneration and multiplication. Shoots rooted maximally (100%) on plant growth regulator-free MS medium containing 2% or 4% sucrose. Increased sucrose content improved plant acclimation in the greenhouse.

Inner scales excised from dormant bulbs of the short-day `Texas Grano 1015Y' onion (Allium cepa L.) were cultured in vitro and leaf growth was examined. Light promoted leaf growth, but no differences in leaf growth were observed for media pH between 4 and 7. Leaf growth rate in darkness was highest at 24C, reduced at 15C, and greatly reduced at SC. Kinetin promoted leaf growth at 1, 10, and 100 μm. IAA was effective at 1 and 10 μM, but not at 0.1 and 100 μm. GA3 promoted growth at 0.1 μM. No inhibitory effects of ABA on leaf growth could be detected. Chemical names used: 1-H-indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellic acid (GA3), 6-furfurylaminopurine (Kinetin).

Penggunaan NAA (Napthalene Acetic Acid) dan Kinetin (6-furfurylaminopurine) pada Induksi Tunas Kubis Bunga Putih (Brassicaoleraceae L. var. Botrytis) secara in-vitro(Use of NAA (Naphtalene acetic acid) and Kinetin (6-furfurylaminopurine)For In-Vitro Shoot Induction of White Cabbage (Brassica oleraceae L. var.Botrytis)Desmin Tabuni1*), Jeany Sh. Polii-Mandang1), Wenny Tilaar1)1) Pascasarjana Program Studi Agronomi Unstrat Manado, 95115*Email korespondensi: desmintabuni2394@gmail.comDiterima 7 Agustus 2018, diterima untuk dipublikasikan 31 Agustus 2018AbstrakTujuan penelitian ini ialah untuk menentukan konsentrasi zat pengaturtumbuh (ZPT) NAA dan kinetin yang terbaik untuk induksi tunas kubis bungaputih (Brassica oleraceae L. var. Botrytis) secara in vitro. Penelitian inidilaksanakan di Laboratorium Bioteknologi Fakultas Pertanian Universitas SamRatulangi Manado, pada bulan Maret – Oktober 2018. Rancangan penelitianyaitu Percobaan Faktorial dalam Rancangan Acak Lengkap (RAL) yang terdiridari 16 kombinasi perlakuan dan diulang sebanyak 5 kali. Hasil analisis ragammemperlihatkan bahwa perlakuan kinetin 0 ppm menghasilkan tinggi tanamanterbesar, yaitu 5,31cm pada umur 4 minggu setelah kultur (MSK). Perlakuan NAA0 ppm menghasilkan jumlah daun terbanyak pada umur 2 MSK (2,2) danterbanyak pada umur 3 MSK (3,10). Perlakuan kinetin 0 ppm menghasilkanjumlah daun terbanyak pada umur 4 MSK (3,75). Perlakuan kinetin 3 ppmmenghasilkan jumlah tunas terbanyak pada umur 4 MSK (4,95). Jumlah tunasterbanyak pada perlakuan kombinasi NAA 0,3 ppm dan kinetin 2 ppm adalah 1,8pada umur 2 MSK.Kata Kunci : kubis, induksi tunas, NAA dan Kinetin, in vitroAbstractThe aim of this study was to determine the optimum concentration of NAAand kinetin for in vitro shoot induction of white cabbage (Brassica oleraceae L.var. Botrytis). This research was carried out in the Biotechnology Laboratory,Agricultural Faculty, Sam Ratulangi University, Manado in March - October 2018.Experimental design in this study was a Factorial Design in CompletelyRandomized Design that consisted of 16 treatment combinations with 5replication. The results of ANOVA showed that 0 ppm kinetin resulted in thelargest plant height, i.e 5.31cm at 4 weeks after culture. The highest leaf numberswere observed at 0 ppm NAA, i.e 2.2 at 2 weeks after culture and 3.10 at 3weeks after culture. The highest leaf numbers was also observed at 0 ppmkinetin, i.e 3.75 at 4 weeks after culture. The treatment of 3 ppm kinetin resultedin the highest shoot number (4.95) at 4 weeks after culture. The highest shootnumber at combination of 0.3 ppm NAA and 2 ppm kinetin was 1.8 at 2 4 weeksafter culture.Keywords: Cabbage, Shoot Induction, NAA and Kinetin, in vitro

Citrus macrophylla is an important citrus rootstock for Mexican lemon (Citrus aurantifolia S.). This study was conducted to select explant type and to optimize cultural requirements for induction callus of C. macrophylla in vitro. The explants tested were leaf, epicotyl, cotyledon, and root segments excised under sterile conditions from 4-week-old nucellar seedlings. The various medium comprising either basal of Murashige and Skoog (MS) or Murashige and Tucker (MT) salts suplemented with various concentrations of plant growth regulators, including naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-furfurylaminopurine (kinetin) were used for the establishment of the explants. All cultured explants initiated callus from the cut ends after 2 weeks, when cultured on a modified MT medium suplemented with 6 mg NAA and 0.2 mg kinetin; cotyledon segments were the best explant for callus induction and development (43 mm2). Root segments were the lowest explants for callus induction.